This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_d0M-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1845.121 CPU sec, 63.884 real sec
[M::process] read 678238 sequences (100000092 bp) in 0.988 CPU sec, 0.989 real sec...
[M::process] read 678158 sequences (100000110 bp) in 1.664 CPU sec, 1.710 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286053, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 311, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (333.82, 154.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677952 sequences (100000107 bp) in 1.662 CPU sec, 1.689 real sec...
[M::process] read 677664 sequences (100000029 bp) in 1.625 CPU sec, 1.665 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285443, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 315, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (336.21, 155.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678238 reads in 177.039 CPU sec, 5.702 real sec
[M::mem_process_seqs] Processed 678158 reads in 108.256 CPU sec, 3.495 real sec
sorting block 0, #reads = 1082312 mem = 536878885 peak = 607526912
[M::process] read 677378 sequences (100000177 bp) in 2.185 CPU sec, 2.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286057, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 317, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (338.45, 156.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677102 sequences (100000155 bp) in 1.772 CPU sec, 1.780 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285750, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 315, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (336.44, 155.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677664 reads in 168.585 CPU sec, 5.396 real sec
[M::mem_process_seqs] Processed 677952 reads in 185.007 CPU sec, 5.938 real sec
sorting block 1, #reads = 1082309 mem = 536883064 peak = 739647488
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285115, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 320, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (341.61, 158.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676888 sequences (100000194 bp) in 1.996 CPU sec, 2.334 real sec...
[M::process] read 676276 sequences (100000190 bp) in 1.711 CPU sec, 1.745 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285148, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 323, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (343.93, 158.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (447, 477, 1432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3402)
[M::mem_pestat] mean and std.dev: (743.83, 494.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4387)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 677378 reads in 168.522 CPU sec, 5.424 real sec
[M::mem_process_seqs] Processed 677102 reads in 107.118 CPU sec, 3.414 real sec
sorting block 2, #reads = 1081554 mem = 536881904 peak = 840581120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285498, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (346.54, 159.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676562 sequences (100000056 bp) in 2.379 CPU sec, 2.638 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284709, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (350.56, 161.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676328 sequences (100000017 bp) in 1.799 CPU sec, 1.813 real sec...
[M::mem_process_seqs] Processed 676888 reads in 166.073 CPU sec, 5.336 real sec
sorting block 3, #reads = 1081845 mem = 536878312 peak = 840581120
[M::mem_process_seqs] Processed 676276 reads in 109.808 CPU sec, 3.504 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284924, 1, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 282, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 502)
[M::mem_pestat] mean and std.dev: (274.00, 60.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 601)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (351.80, 162.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 4, #reads = 1082447 mem = 536877535 peak = 904163328
[M::process] read 676002 sequences (100000285 bp) in 2.418 CPU sec, 2.661 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284766, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (353.39, 162.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675862 sequences (100000029 bp) in 1.740 CPU sec, 1.822 real sec...
[M::mem_process_seqs] Processed 676562 reads in 165.572 CPU sec, 5.289 real sec
[M::mem_process_seqs] Processed 676328 reads in 107.496 CPU sec, 3.410 real sec
sorting block 5, #reads = 1081516 mem = 536877844 peak = 904163328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284944, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 202, 203)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343)
[M::mem_pestat] mean and std.dev: (174.88, 35.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 413)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (354.15, 163.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675896 sequences (100000030 bp) in 2.626 CPU sec, 2.886 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284976, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (353.58, 161.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676002 reads in 165.814 CPU sec, 5.295 real sec
[M::process] read 675988 sequences (100000233 bp) in 1.973 CPU sec, 2.087 real sec...
[M::mem_process_seqs] Processed 675862 reads in 106.313 CPU sec, 3.374 real sec
sorting block 6, #reads = 1081385 mem = 536877458 peak = 904163328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284660, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (351.39, 161.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675982 sequences (100000297 bp) in 2.383 CPU sec, 2.562 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284631, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (350.50, 160.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675888 sequences (100000207 bp) in 1.810 CPU sec, 1.818 real sec...
[M::mem_process_seqs] Processed 675896 reads in 168.780 CPU sec, 5.392 real sec
[M::mem_process_seqs] Processed 675988 reads in 107.439 CPU sec, 3.405 real sec
sorting block 7, #reads = 1081379 mem = 536878552 peak = 904163328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284948, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (350.02, 160.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676120 sequences (100000174 bp) in 2.718 CPU sec, 2.935 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284975, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (349.11, 159.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676156 sequences (100000092 bp) in 1.831 CPU sec, 1.837 real sec...
[M::mem_process_seqs] Processed 675982 reads in 169.447 CPU sec, 5.406 real sec
sorting block 8, #reads = 1080503 mem = 536878404 peak = 904163328
[M::mem_process_seqs] Processed 675888 reads in 109.439 CPU sec, 3.464 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285485, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 326, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (348.30, 160.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 9, #reads = 1081717 mem = 536877788 peak = 904163328
[M::process] read 675980 sequences (100000298 bp) in 2.454 CPU sec, 2.633 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285137, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (348.40, 160.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675900 sequences (100000062 bp) in 1.868 CPU sec, 1.896 real sec...
[M::mem_process_seqs] Processed 676120 reads in 169.647 CPU sec, 5.418 real sec
[M::mem_process_seqs] Processed 676156 reads in 109.761 CPU sec, 3.481 real sec
sorting block 10, #reads = 1081820 mem = 536877628 peak = 904163328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284416, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (350.14, 161.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675740 sequences (100000140 bp) in 2.973 CPU sec, 3.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284439, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 205, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (305.09, 148.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (351.01, 161.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675980 reads in 169.094 CPU sec, 5.408 real sec
[M::mem_process_seqs] Processed 675900 reads in 108.967 CPU sec, 3.452 real sec
[M::process] read 675742 sequences (100000192 bp) in 2.065 CPU sec, 2.144 real sec...
sorting block 11, #reads = 1082155 mem = 536881596 peak = 904163328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284086, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (352.54, 161.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675286 sequences (100000128 bp) in 2.435 CPU sec, 2.813 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284658, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (350.07, 159.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675740 reads in 169.991 CPU sec, 5.488 real sec
[M::mem_process_seqs] Processed 675742 reads in 109.596 CPU sec, 3.499 real sec
[M::process] read 675302 sequences (100000202 bp) in 1.921 CPU sec, 1.992 real sec...
sorting block 12, #reads = 1081174 mem = 536878611 peak = 904163328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284442, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (352.21, 159.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675470 sequences (100000012 bp) in 2.969 CPU sec, 3.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284168, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (352.27, 159.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675286 reads in 167.727 CPU sec, 5.393 real sec
[M::mem_process_seqs] Processed 675302 reads in 110.355 CPU sec, 3.509 real sec
sorting block 13, #reads = 1080507 mem = 536879935 peak = 904163328
[M::process] read 674836 sequences (100000291 bp) in 2.206 CPU sec, 2.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284607, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (351.82, 159.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1080533 mem = 536879580 peak = 913895424
[M::process] read 674908 sequences (100000206 bp) in 2.409 CPU sec, 2.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284085, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (354.37, 160.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675470 reads in 167.051 CPU sec, 5.388 real sec
[M::mem_process_seqs] Processed 674836 reads in 108.844 CPU sec, 3.454 real sec
[M::process] read 674722 sequences (100000012 bp) in 2.514 CPU sec, 2.696 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284071, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (356.59, 161.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 15, #reads = 1080822 mem = 536879895 peak = 913895424
[M::process] read 674874 sequences (100000031 bp) in 2.770 CPU sec, 3.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283926, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 339, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (359.59, 163.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674908 reads in 164.065 CPU sec, 5.287 real sec
[M::mem_process_seqs] Processed 674722 reads in 107.713 CPU sec, 3.423 real sec
[M::process] read 674550 sequences (100000129 bp) in 2.745 CPU sec, 2.923 real sec...
sorting block 16, #reads = 1080944 mem = 536877590 peak = 913895424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284629, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 340, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (359.32, 163.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677662 sequences (100000076 bp) in 2.321 CPU sec, 2.756 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283871, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 343, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (361.43, 163.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674874 reads in 167.052 CPU sec, 5.398 real sec
[M::mem_process_seqs] Processed 674550 reads in 110.702 CPU sec, 3.543 real sec
sorting block 17, #reads = 1080987 mem = 536884694 peak = 913895424
[M::process] read 678396 sequences (100000126 bp) in 2.890 CPU sec, 3.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285030, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 321, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (341.29, 157.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677922 sequences (100000141 bp) in 2.443 CPU sec, 2.740 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285254, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (340.01, 158.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677662 reads in 170.256 CPU sec, 5.481 real sec
[M::mem_process_seqs] Processed 678396 reads in 110.929 CPU sec, 3.541 real sec
sorting block 18, #reads = 1079223 mem = 536878328 peak = 913895424
[M::process] read 677886 sequences (100000051 bp) in 2.708 CPU sec, 2.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285218, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 320, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (341.67, 159.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1084514 mem = 536877123 peak = 913895424
[M::process] read 677550 sequences (100000275 bp) in 2.376 CPU sec, 2.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285253, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 320, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (341.07, 158.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677922 reads in 167.211 CPU sec, 5.417 real sec
[M::mem_process_seqs] Processed 677886 reads in 109.493 CPU sec, 3.530 real sec
[M::process] read 677210 sequences (100000292 bp) in 2.457 CPU sec, 2.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285143, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 323, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (343.20, 158.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 20, #reads = 1083531 mem = 536887004 peak = 913895424
[M::process] read 677142 sequences (100000252 bp) in 2.854 CPU sec, 3.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284326, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 325, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (345.41, 159.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 405, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (319.60, 108.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 677550 reads in 171.764 CPU sec, 5.524 real sec
[M::mem_process_seqs] Processed 677210 reads in 110.402 CPU sec, 3.514 real sec
[M::process] read 676758 sequences (100000034 bp) in 2.240 CPU sec, 2.371 real sec...
sorting block 21, #reads = 1082861 mem = 536878718 peak = 913895424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284740, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 326, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (346.53, 159.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676392 sequences (100000190 bp) in 2.423 CPU sec, 2.825 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284722, 1, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (350.69, 161.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 398, 2416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6904)
[M::mem_pestat] mean and std.dev: (785.71, 910.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9148)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 677142 reads in 167.804 CPU sec, 5.439 real sec
[M::mem_process_seqs] Processed 676758 reads in 108.286 CPU sec, 3.463 real sec
sorting block 22, #reads = 1083207 mem = 536881143 peak = 913895424
[M::process] read 676262 sequences (100000289 bp) in 2.581 CPU sec, 2.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284473, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (351.41, 161.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676288 sequences (100000066 bp) in 2.763 CPU sec, 3.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285074, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (355.93, 164.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676392 reads in 165.923 CPU sec, 5.378 real sec
[M::mem_process_seqs] Processed 676262 reads in 107.716 CPU sec, 3.451 real sec
sorting block 23, #reads = 1082917 mem = 536879204 peak = 913895424
[M::process] read 676214 sequences (100000157 bp) in 2.576 CPU sec, 2.747 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284792, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (354.70, 163.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1082635 mem = 536882417 peak = 920244224
[M::process] read 676054 sequences (100000220 bp) in 2.375 CPU sec, 2.867 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285367, 4, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (353, 412, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (235, 530)
[M::mem_pestat] mean and std.dev: (397.25, 25.55)
[M::mem_pestat] low and high boundaries for proper pairs: (176, 589)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 335, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (355.44, 164.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676288 reads in 165.960 CPU sec, 5.379 real sec
[M::mem_process_seqs] Processed 676214 reads in 108.803 CPU sec, 3.483 real sec
[M::process] read 676174 sequences (100000124 bp) in 2.723 CPU sec, 2.865 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284396, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 336, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (356.37, 165.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1082878 mem = 536879680 peak = 920244224
[M::process] read 676184 sequences (100000284 bp) in 2.639 CPU sec, 2.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284829, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (355.36, 164.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676054 reads in 172.306 CPU sec, 5.593 real sec
[M::mem_process_seqs] Processed 676174 reads in 112.023 CPU sec, 3.636 real sec
[M::process] read 676152 sequences (100000213 bp) in 2.605 CPU sec, 2.788 real sec...
sorting block 26, #reads = 1082152 mem = 536879886 peak = 920244224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284633, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (354.23, 163.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676500 sequences (100000102 bp) in 2.427 CPU sec, 2.773 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284391, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (351.86, 162.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676184 reads in 171.498 CPU sec, 5.556 real sec
[M::mem_process_seqs] Processed 676152 reads in 112.706 CPU sec, 3.642 real sec
sorting block 27, #reads = 1081025 mem = 536878257 peak = 920244224
[M::process] read 676482 sequences (100000022 bp) in 2.002 CPU sec, 2.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284477, 10, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 325, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (346.30, 159.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 350, 2078)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5878)
[M::mem_pestat] mean and std.dev: (1235.80, 1496.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7778)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676266 sequences (100000159 bp) in 2.686 CPU sec, 2.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284904, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 324, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (345.29, 158.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676500 reads in 172.175 CPU sec, 5.531 real sec
[M::mem_process_seqs] Processed 676482 reads in 110.998 CPU sec, 3.556 real sec
sorting block 28, #reads = 1081370 mem = 536878168 peak = 920244224
[M::process] read 676462 sequences (100000091 bp) in 1.973 CPU sec, 2.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284659, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 325, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (345.75, 158.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 29, #reads = 1080932 mem = 536879273 peak = 959700992
[M::process] read 676072 sequences (100000057 bp) in 2.589 CPU sec, 2.857 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284698, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 325, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (346.30, 159.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676266 reads in 173.690 CPU sec, 5.627 real sec
[M::mem_process_seqs] Processed 676462 reads in 111.898 CPU sec, 3.604 real sec
[M::process] read 675906 sequences (100000159 bp) in 2.213 CPU sec, 2.371 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284954, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 327, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (347.67, 159.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 30, #reads = 1081149 mem = 536882724 peak = 959700992
[M::process] read 675988 sequences (100000080 bp) in 2.916 CPU sec, 3.234 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284054, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (348.30, 159.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676072 reads in 176.511 CPU sec, 5.694 real sec
[M::mem_process_seqs] Processed 675906 reads in 115.737 CPU sec, 3.708 real sec
[M::process] read 675604 sequences (100000231 bp) in 2.185 CPU sec, 2.249 real sec...
sorting block 31, #reads = 1081842 mem = 536880321 peak = 959700992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284827, 6, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 440, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (385.15, 177.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (348.31, 158.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675530 sequences (100000291 bp) in 2.544 CPU sec, 3.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285244, 7, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (352.85, 161.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 338, 1481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3991)
[M::mem_pestat] mean and std.dev: (630.54, 614.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5246)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675988 reads in 173.318 CPU sec, 5.627 real sec
[M::mem_process_seqs] Processed 675604 reads in 114.830 CPU sec, 3.658 real sec
[M::process] read 675730 sequences (100000092 bp) in 2.391 CPU sec, 2.487 real sec...
sorting block 32, #reads = 1081460 mem = 536880009 peak = 959700992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284649, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (355.07, 163.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675362 sequences (100000026 bp) in 2.619 CPU sec, 3.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284460, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (353.79, 162.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675530 reads in 169.829 CPU sec, 5.512 real sec
sorting block 33, #reads = 1081504 mem = 536878659 peak = 959700992
[M::mem_process_seqs] Processed 675730 reads in 113.084 CPU sec, 3.628 real sec
[M::process] read 675340 sequences (100000169 bp) in 2.520 CPU sec, 2.662 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284473, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (355.17, 163.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 34, #reads = 1080451 mem = 536878224 peak = 959700992
[M::process] read 675218 sequences (100000076 bp) in 2.330 CPU sec, 2.831 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284683, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (355.53, 162.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675362 reads in 168.307 CPU sec, 5.479 real sec
[M::mem_process_seqs] Processed 675340 reads in 111.630 CPU sec, 3.605 real sec
[M::process] read 675338 sequences (100000122 bp) in 3.198 CPU sec, 3.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284828, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (359.06, 165.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 35, #reads = 1080781 mem = 536878945 peak = 959700992
[M::process] read 677312 sequences (100000294 bp) in 2.510 CPU sec, 3.012 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284938, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 339, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (360.05, 167.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675218 reads in 169.657 CPU sec, 5.532 real sec
[M::mem_process_seqs] Processed 675338 reads in 111.125 CPU sec, 3.613 real sec
[M::process] read 679038 sequences (100000035 bp) in 3.064 CPU sec, 3.262 real sec...
sorting block 36, #reads = 1080380 mem = 536877996 peak = 959700992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285463, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 326, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (346.36, 160.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678454 sequences (100000202 bp) in 2.266 CPU sec, 2.682 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285707, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (333.19, 153.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677312 reads in 176.961 CPU sec, 5.815 real sec
[M::mem_process_seqs] Processed 679038 reads in 111.652 CPU sec, 3.679 real sec
sorting block 37, #reads = 1079146 mem = 536879207 peak = 959700992
[M::process] read 678552 sequences (100000137 bp) in 3.154 CPU sec, 3.478 real sec...
[M::process] read 678152 sequences (100000206 bp) in 2.350 CPU sec, 2.601 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285175, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 315, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (334.68, 153.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678552 reads in 94.484 CPU sec, 3.202 real sec
sorting block 38, #reads = 1083371 mem = 536878042 peak = 959700992
[M::process] read 677650 sequences (100000125 bp) in 2.823 CPU sec, 3.130 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285697, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 777, 1378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3848)
[M::mem_pestat] mean and std.dev: (636.75, 523.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5083)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 319, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (337.52, 154.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285978, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 316, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (335.67, 154.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678152 reads in 98.537 CPU sec, 3.197 real sec
[M::mem_process_seqs] Processed 678454 reads in 296.014 CPU sec, 9.790 real sec
sorting block 39, #reads = 1083278 mem = 536880111 peak = 959700992
[M::process] read 677386 sequences (100000153 bp) in 2.737 CPU sec, 3.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285133, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 321, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (340.30, 155.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676920 sequences (100000247 bp) in 2.786 CPU sec, 3.233 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285460, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 323, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (340.94, 154.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677650 reads in 180.458 CPU sec, 5.892 real sec
[M::mem_process_seqs] Processed 677386 reads in 119.765 CPU sec, 3.867 real sec
sorting block 40, #reads = 1082721 mem = 536880210 peak = 959700992
[M::process] read 676632 sequences (100000246 bp) in 2.465 CPU sec, 2.684 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284910, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (345.18, 156.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1081787 mem = 536878898 peak = 959700992
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284809, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 326, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (344.94, 155.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676414 sequences (100000218 bp) in 2.902 CPU sec, 3.439 real sec...
[M::mem_process_seqs] Processed 676920 reads in 174.971 CPU sec, 5.671 real sec
[M::mem_process_seqs] Processed 676632 reads in 117.535 CPU sec, 3.768 real sec
[M::process] read 676318 sequences (100000135 bp) in 2.798 CPU sec, 3.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284617, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (345.73, 156.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 42, #reads = 1081952 mem = 536878853 peak = 959700992
[M::process] read 676424 sequences (100000126 bp) in 2.745 CPU sec, 3.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284609, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (349.09, 157.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676414 reads in 178.339 CPU sec, 5.787 real sec
[M::mem_process_seqs] Processed 676318 reads in 116.325 CPU sec, 3.736 real sec
sorting block 43, #reads = 1081681 mem = 536878265 peak = 959700992
[M::process] read 676304 sequences (100000262 bp) in 3.007 CPU sec, 3.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284586, 10, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 321, 723)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1797)
[M::mem_pestat] mean and std.dev: (388.45, 245.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2334)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (347.52, 156.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (508, 824, 824)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1456)
[M::mem_pestat] mean and std.dev: (592.50, 237.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1772)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 675884 sequences (100000030 bp) in 2.584 CPU sec, 3.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284732, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 182, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (136, 251)
[M::mem_pestat] mean and std.dev: (190.18, 18.97)
[M::mem_pestat] low and high boundaries for proper pairs: (113, 274)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (349.35, 158.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676424 reads in 179.216 CPU sec, 5.787 real sec
[M::mem_process_seqs] Processed 676304 reads in 116.109 CPU sec, 3.711 real sec
sorting block 44, #reads = 1081712 mem = 536878423 peak = 959700992
[M::process] read 676176 sequences (100000297 bp) in 2.817 CPU sec, 2.990 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284752, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (351.54, 159.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676564 sequences (100000035 bp) in 2.707 CPU sec, 2.932 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285185, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 332, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (350.37, 158.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675884 reads in 177.872 CPU sec, 5.774 real sec
[M::mem_process_seqs] Processed 676176 reads in 112.450 CPU sec, 3.634 real sec
sorting block 45, #reads = 1080719 mem = 536880190 peak = 959700992
[M::process] read 676400 sequences (100000290 bp) in 2.673 CPU sec, 2.902 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284518, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 324, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (345.26, 158.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1082938 mem = 536877453 peak = 962076672
[M::process] read 676104 sequences (100000228 bp) in 2.624 CPU sec, 3.076 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284790, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (347.25, 158.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676564 reads in 181.025 CPU sec, 5.886 real sec
[M::mem_process_seqs] Processed 676400 reads in 117.213 CPU sec, 3.807 real sec
[M::process] read 676256 sequences (100000180 bp) in 2.670 CPU sec, 2.884 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283983, 4, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 326, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (346.48, 157.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 363, 3704)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10676)
[M::mem_pestat] mean and std.dev: (1137.24, 1431.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14162)
[M::mem_pestat] skip orientation RR
sorting block 47, #reads = 1081346 mem = 536878238 peak = 962076672
[M::process] read 676504 sequences (100000077 bp) in 2.550 CPU sec, 2.868 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284215, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 326, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (346.06, 156.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676104 reads in 180.960 CPU sec, 5.933 real sec
[M::process] read 676022 sequences (100000122 bp) in 2.832 CPU sec, 3.071 real sec...
sorting block 48, #reads = 1081459 mem = 536881636 peak = 962076672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284802, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (346.25, 158.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676504 reads in 99.071 CPU sec, 3.181 real sec
[M::process] read 676238 sequences (100000018 bp) in 2.332 CPU sec, 2.582 real sec...
[M::mem_process_seqs] Processed 676256 reads in 272.924 CPU sec, 8.849 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284265, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (349.28, 159.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1081067 mem = 536878638 peak = 962076672
[M::process] read 676008 sequences (100000121 bp) in 2.889 CPU sec, 3.126 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284626, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 326, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (346.31, 157.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676022 reads in 196.220 CPU sec, 6.316 real sec
[M::mem_process_seqs] Processed 676238 reads in 114.354 CPU sec, 3.657 real sec
[M::process] read 675858 sequences (100000160 bp) in 2.742 CPU sec, 2.936 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284398, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 326, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (346.13, 156.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 50, #reads = 1080467 mem = 536881396 peak = 962076672
[M::process] read 675810 sequences (100000058 bp) in 2.514 CPU sec, 2.941 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284222, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (347.84, 157.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676008 reads in 179.241 CPU sec, 5.790 real sec
[M::mem_process_seqs] Processed 675858 reads in 117.294 CPU sec, 3.743 real sec
sorting block 51, #reads = 1080177 mem = 536878219 peak = 962076672
[M::process] read 675982 sequences (100000065 bp) in 2.715 CPU sec, 2.882 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284614, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (351.62, 160.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675692 sequences (100000164 bp) in 2.776 CPU sec, 3.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284584, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (253, 253, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (233, 283)
[M::mem_pestat] mean and std.dev: (256.75, 4.84)
[M::mem_pestat] low and high boundaries for proper pairs: (223, 293)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (351.67, 162.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675810 reads in 178.688 CPU sec, 5.761 real sec
[M::mem_process_seqs] Processed 675982 reads in 117.653 CPU sec, 3.757 real sec
sorting block 52, #reads = 1080485 mem = 536878363 peak = 962076672
[M::process] read 675812 sequences (100000211 bp) in 2.634 CPU sec, 2.845 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283908, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (353.52, 162.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1078631 mem = 536877568 peak = 962076672
[M::process] read 675734 sequences (100000039 bp) in 2.564 CPU sec, 3.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284671, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (352.58, 162.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 1420, 2682)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7540)
[M::mem_pestat] mean and std.dev: (1565.70, 1022.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9969)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675692 reads in 172.408 CPU sec, 5.597 real sec
[M::mem_process_seqs] Processed 675812 reads in 114.075 CPU sec, 3.663 real sec
[M::process] read 675774 sequences (100000146 bp) in 2.331 CPU sec, 2.563 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284380, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (566, 566, 946)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1706)
[M::mem_pestat] mean and std.dev: (664.08, 255.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2086)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 332, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (354.38, 164.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 54, #reads = 1080439 mem = 536881216 peak = 962076672
[M::process] read 678598 sequences (100000299 bp) in 2.798 CPU sec, 3.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284801, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (354.29, 163.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675734 reads in 176.150 CPU sec, 5.778 real sec
[M::mem_process_seqs] Processed 675774 reads in 116.166 CPU sec, 3.786 real sec
[M::process] read 678414 sequences (100000233 bp) in 2.753 CPU sec, 2.962 real sec...
sorting block 55, #reads = 1080043 mem = 536877973 peak = 962076672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285038, 1, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 430, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (342.00, 172.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 313, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (333.33, 153.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 677972 sequences (100000175 bp) in 2.770 CPU sec, 3.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284676, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 537, 549)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1195)
[M::mem_pestat] mean and std.dev: (435.67, 255.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1518)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 317, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (337.16, 155.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 678598 reads in 176.884 CPU sec, 5.754 real sec
[M::mem_process_seqs] Processed 678414 reads in 113.389 CPU sec, 3.678 real sec
sorting block 56, #reads = 1078723 mem = 536879177 peak = 962076672
[M::process] read 677950 sequences (100000123 bp) in 2.778 CPU sec, 3.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284626, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 319, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (339.42, 156.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677564 sequences (100000013 bp) in 2.842 CPU sec, 3.071 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284893, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 320, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (339.71, 156.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677972 reads in 173.692 CPU sec, 5.620 real sec
[M::mem_process_seqs] Processed 677950 reads in 114.305 CPU sec, 3.650 real sec
sorting block 57, #reads = 1084071 mem = 536880485 peak = 962076672
[M::process] read 677522 sequences (100000173 bp) in 2.770 CPU sec, 2.916 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284716, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 321, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (340.81, 156.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1083339 mem = 536882549 peak = 962076672
[M::process] read 677220 sequences (100000269 bp) in 2.430 CPU sec, 2.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284371, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (341.69, 156.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677564 reads in 178.262 CPU sec, 5.760 real sec
[M::mem_process_seqs] Processed 677522 reads in 116.813 CPU sec, 3.703 real sec
[M::process] read 676840 sequences (100000169 bp) in 2.349 CPU sec, 2.566 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284160, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 324, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (343.54, 156.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 59, #reads = 1082385 mem = 536878535 peak = 962076672
[M::process] read 676630 sequences (100000221 bp) in 2.896 CPU sec, 3.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284606, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 134, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734)
[M::mem_pestat] mean and std.dev: (201.29, 99.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (346.75, 157.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 677220 reads in 180.175 CPU sec, 5.804 real sec
[M::mem_process_seqs] Processed 676840 reads in 117.718 CPU sec, 3.749 real sec
[M::process] read 676624 sequences (100000117 bp) in 2.406 CPU sec, 2.608 real sec...
sorting block 60, #reads = 1082801 mem = 536878054 peak = 962076672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284304, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (348.96, 158.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676596 sequences (100000166 bp) in 2.354 CPU sec, 2.773 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284659, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (347.70, 157.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676630 reads in 178.305 CPU sec, 5.757 real sec
[M::mem_process_seqs] Processed 676624 reads in 117.024 CPU sec, 3.730 real sec
[M::process] read 676362 sequences (100000038 bp) in 2.330 CPU sec, 2.405 real sec...
sorting block 61, #reads = 1082982 mem = 536880158 peak = 989081600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284942, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (254, 1267, 1267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3293)
[M::mem_pestat] mean and std.dev: (939.40, 501.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4306)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (347.70, 157.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676358 sequences (100000022 bp) in 2.739 CPU sec, 2.888 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284232, 8, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (1475, 1475, 1475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1475, 1475)
[M::mem_pestat] mean and std.dev: (1475.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1475, 1475)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (349.54, 158.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676596 reads in 176.163 CPU sec, 5.697 real sec
[M::mem_process_seqs] Processed 676362 reads in 113.875 CPU sec, 3.656 real sec
sorting block 62, #reads = 1081576 mem = 536878803 peak = 989081600
[M::process] read 676404 sequences (100000180 bp) in 2.868 CPU sec, 2.996 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284358, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (348.03, 156.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 63, #reads = 1082473 mem = 536878951 peak = 989081600
[M::process] read 676628 sequences (100000231 bp) in 2.403 CPU sec, 2.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284846, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (349.40, 159.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676358 reads in 185.196 CPU sec, 6.020 real sec
[M::mem_process_seqs] Processed 676404 reads in 116.588 CPU sec, 3.785 real sec
[M::process] read 677878 sequences (100000054 bp) in 2.116 CPU sec, 2.423 real sec...
sorting block 64, #reads = 1080807 mem = 536878980 peak = 989081600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284094, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (264, 264, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (351.30, 155.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (349.31, 160.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676566 sequences (100000187 bp) in 2.871 CPU sec, 3.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285334, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 313, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (336.79, 156.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676628 reads in 184.151 CPU sec, 5.925 real sec
[M::process] read 676732 sequences (100000072 bp) in 2.070 CPU sec, 2.103 real sec...
[M::mem_process_seqs] Processed 677878 reads in 116.150 CPU sec, 3.724 real sec
sorting block 65, #reads = 1080715 mem = 536881522 peak = 989081600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284219, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 324, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (344.21, 157.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676532 sequences (100000227 bp) in 2.540 CPU sec, 2.912 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284200, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 321, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (340.95, 154.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676566 reads in 182.853 CPU sec, 5.899 real sec
[M::process] read 676378 sequences (100000099 bp) in 1.959 CPU sec, 2.017 real sec...
[M::mem_process_seqs] Processed 676732 reads in 118.097 CPU sec, 3.755 real sec
sorting block 66, #reads = 1080511 mem = 536878556 peak = 989081600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285404, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 322, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (341.98, 154.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676410 sequences (100000162 bp) in 2.718 CPU sec, 3.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284542, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 321, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (340.77, 153.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676532 reads in 182.776 CPU sec, 5.883 real sec
[M::process] read 676484 sequences (100000113 bp) in 1.966 CPU sec, 1.990 real sec...
sorting block 67, #reads = 1079604 mem = 536884938 peak = 989081600
[M::mem_process_seqs] Processed 676378 reads in 117.827 CPU sec, 3.761 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284768, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 322, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (341.45, 153.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 68, #reads = 1081775 mem = 536879377 peak = 1016344576
[M::process] read 676344 sequences (100000169 bp) in 2.396 CPU sec, 2.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284369, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 321, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (340.21, 153.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676410 reads in 181.458 CPU sec, 5.829 real sec
[M::process] read 676232 sequences (100000014 bp) in 2.055 CPU sec, 2.077 real sec...
[M::mem_process_seqs] Processed 676484 reads in 119.679 CPU sec, 3.814 real sec
sorting block 69, #reads = 1080339 mem = 536879357 peak = 1016344576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284871, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 320, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (339.64, 152.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675976 sequences (100000295 bp) in 2.927 CPU sec, 3.390 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284578, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 319, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (338.70, 151.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676344 reads in 175.458 CPU sec, 5.631 real sec
[M::mem_process_seqs] Processed 676232 reads in 114.968 CPU sec, 3.657 real sec
[M::process] read 675760 sequences (100000297 bp) in 2.190 CPU sec, 2.365 real sec...
sorting block 70, #reads = 1080060 mem = 536882887 peak = 1016344576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284576, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 325, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (344.09, 154.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 260, 265)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (208, 303)
[M::mem_pestat] mean and std.dev: (251.44, 17.17)
[M::mem_pestat] low and high boundaries for proper pairs: (183, 322)
[M::mem_pestat] skip orientation RR
[M::process] read 675740 sequences (100000180 bp) in 2.355 CPU sec, 2.776 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283845, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (348.34, 157.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675976 reads in 176.700 CPU sec, 5.732 real sec
[M::mem_process_seqs] Processed 675760 reads in 115.239 CPU sec, 3.701 real sec
[M::process] read 675834 sequences (100000019 bp) in 2.119 CPU sec, 2.173 real sec...
sorting block 71, #reads = 1079805 mem = 536878189 peak = 1016344576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284815, 5, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 246, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (267.42, 70.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (350.01, 159.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (583, 583, 1035)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1939)
[M::mem_pestat] mean and std.dev: (615.37, 332.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2391)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 675472 sequences (100000121 bp) in 2.863 CPU sec, 3.219 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283660, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (350.50, 160.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675740 reads in 174.962 CPU sec, 5.626 real sec
sorting block 72, #reads = 1079450 mem = 536879155 peak = 1016344576
[M::mem_process_seqs] Processed 675834 reads in 114.554 CPU sec, 3.628 real sec
[M::process] read 677224 sequences (100000075 bp) in 2.317 CPU sec, 2.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284959, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (354.52, 162.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 73, #reads = 1080091 mem = 536879124 peak = 1016344576
[M::process] read 681586 sequences (100000064 bp) in 2.515 CPU sec, 2.871 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285704, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (344.29, 160.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675472 reads in 174.344 CPU sec, 5.687 real sec
[M::mem_process_seqs] Processed 677224 reads in 117.868 CPU sec, 3.804 real sec
[M::process] read 680426 sequences (100000194 bp) in 2.423 CPU sec, 2.550 real sec...
sorting block 74, #reads = 1079508 mem = 536880026 peak = 1016344576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287273, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 287, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (318.07, 154.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679284 sequences (100000064 bp) in 2.799 CPU sec, 3.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286707, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 297, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (325.09, 155.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681586 reads in 191.126 CPU sec, 6.158 real sec
[M::mem_process_seqs] Processed 680426 reads in 123.760 CPU sec, 3.963 real sec
[M::process] read 678536 sequences (100000005 bp) in 2.051 CPU sec, 2.155 real sec...
sorting block 75, #reads = 1080822 mem = 536877490 peak = 1016344576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285755, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 308, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (334.59, 159.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1120, 1393, 1811)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3193)
[M::mem_pestat] mean and std.dev: (1449.36, 520.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3884)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 678084 sequences (100000279 bp) in 2.699 CPU sec, 3.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285514, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 313, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (338.86, 161.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679284 reads in 181.361 CPU sec, 5.841 real sec
[M::mem_process_seqs] Processed 678536 reads in 119.693 CPU sec, 3.803 real sec
[M::process] read 677718 sequences (100000046 bp) in 2.154 CPU sec, 2.265 real sec...
sorting block 76, #reads = 1081108 mem = 536878438 peak = 1016344576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285873, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 318, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (342.52, 162.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677284 sequences (100000213 bp) in 2.623 CPU sec, 2.863 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285574, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 321, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (344.54, 162.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678084 reads in 175.927 CPU sec, 5.668 real sec
sorting block 77, #reads = 1081658 mem = 536878170 peak = 1016344576
[M::process] read 677380 sequences (100000050 bp) in 2.122 CPU sec, 2.156 real sec...
[M::mem_process_seqs] Processed 677718 reads in 114.705 CPU sec, 3.635 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285593, 2, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 324, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (346.74, 162.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (340, 353, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (314, 379)
[M::mem_pestat] mean and std.dev: (349.44, 7.01)
[M::mem_pestat] low and high boundaries for proper pairs: (301, 392)
[M::mem_pestat] skip orientation RR
sorting block 78, #reads = 1081366 mem = 536880395 peak = 1058279424
[M::process] read 677218 sequences (100000110 bp) in 2.665 CPU sec, 3.129 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285193, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 325, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (347.27, 162.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677284 reads in 178.732 CPU sec, 5.775 real sec
[M::mem_process_seqs] Processed 677380 reads in 116.652 CPU sec, 3.707 real sec
[M::process] read 677082 sequences (100000183 bp) in 2.378 CPU sec, 2.443 real sec...
sorting block 79, #reads = 1080233 mem = 536879753 peak = 1058279424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285283, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 326, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (348.68, 163.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 312, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (315.08, 167.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1332)
[M::mem_pestat] skip orientation RR
[M::process] read 676762 sequences (100000151 bp) in 2.825 CPU sec, 3.185 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285428, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 329, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (350.59, 163.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677218 reads in 180.729 CPU sec, 5.837 real sec
[M::process] read 676686 sequences (100000236 bp) in 2.102 CPU sec, 2.148 real sec...
[M::mem_process_seqs] Processed 677082 reads in 118.098 CPU sec, 3.778 real sec
sorting block 80, #reads = 1080388 mem = 536880855 peak = 1058279424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285321, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (263, 366, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (351.50, 161.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 328, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (349.32, 161.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676372 sequences (100000295 bp) in 2.534 CPU sec, 3.102 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285199, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 328, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (349.50, 161.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676762 reads in 186.305 CPU sec, 6.047 real sec
[M::process] read 676398 sequences (100000272 bp) in 2.038 CPU sec, 2.100 real sec...
[M::mem_process_seqs] Processed 676686 reads in 120.486 CPU sec, 3.865 real sec
sorting block 81, #reads = 1079271 mem = 536878440 peak = 1058279424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285481, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (350.04, 160.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676460 sequences (100000187 bp) in 2.962 CPU sec, 3.385 real sec...
sorting block 82, #reads = 1079220 mem = 536878519 peak = 1058279424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284756, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 228, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428)
[M::mem_pestat] mean and std.dev: (175.40, 54.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 525)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (349.84, 160.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676372 reads in 184.947 CPU sec, 5.985 real sec
[M::process] read 676622 sequences (100000132 bp) in 2.106 CPU sec, 2.218 real sec...
[M::mem_process_seqs] Processed 676398 reads in 122.217 CPU sec, 3.915 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284667, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (349.97, 161.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 83, #reads = 1079628 mem = 536881215 peak = 1058279424
[M::process] read 676584 sequences (100000170 bp) in 2.634 CPU sec, 3.092 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285613, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 327, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (348.76, 160.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676460 reads in 184.010 CPU sec, 5.930 real sec
[M::process] read 676306 sequences (100000106 bp) in 2.044 CPU sec, 2.063 real sec...
[M::mem_process_seqs] Processed 676622 reads in 122.026 CPU sec, 3.886 real sec
sorting block 84, #reads = 1079378 mem = 536878235 peak = 1058279424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285425, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 325, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (346.83, 159.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 286, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (287.80, 132.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RR
[M::process] read 676160 sequences (100000188 bp) in 2.822 CPU sec, 3.111 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284785, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (348.97, 160.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676584 reads in 185.598 CPU sec, 5.950 real sec
[M::process] read 676256 sequences (100000262 bp) in 2.230 CPU sec, 2.277 real sec...
[M::mem_process_seqs] Processed 676306 reads in 121.810 CPU sec, 3.881 real sec
sorting block 85, #reads = 1079006 mem = 536878614 peak = 1058279424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284783, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (350.01, 160.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676218 sequences (100000165 bp) in 2.576 CPU sec, 2.840 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285201, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 326, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (347.71, 159.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676160 reads in 184.961 CPU sec, 5.951 real sec
[M::process] read 676298 sequences (100000067 bp) in 2.136 CPU sec, 2.199 real sec...
sorting block 86, #reads = 1077461 mem = 536878303 peak = 1058279424
[M::mem_process_seqs] Processed 676256 reads in 121.934 CPU sec, 3.889 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285605, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (348.48, 159.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 87, #reads = 1077582 mem = 536883643 peak = 1058279424
[M::process] read 676058 sequences (100000155 bp) in 2.933 CPU sec, 3.292 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285324, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 326, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (347.33, 158.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676218 reads in 185.300 CPU sec, 5.958 real sec
[M::process] read 676206 sequences (100000035 bp) in 2.043 CPU sec, 2.091 real sec...
[M::mem_process_seqs] Processed 676298 reads in 122.301 CPU sec, 3.912 real sec
sorting block 88, #reads = 1078650 mem = 536878155 peak = 1058279424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284856, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (348.13, 158.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676074 sequences (100000059 bp) in 2.569 CPU sec, 2.913 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284933, 0, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (348.29, 159.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (388, 566, 566)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (32, 922)
[M::mem_pestat] mean and std.dev: (466.93, 122.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation RR
[M::process] read 676024 sequences (100000035 bp) in 1.974 CPU sec, 1.998 real sec...
[M::mem_process_seqs] Processed 676058 reads in 180.773 CPU sec, 5.807 real sec
[M::mem_process_seqs] Processed 676206 reads in 118.695 CPU sec, 3.785 real sec
sorting block 89, #reads = 1078467 mem = 536878935 peak = 1058279424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284844, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (350.80, 161.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675614 sequences (100000197 bp) in 3.017 CPU sec, 3.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284656, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (352.97, 163.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676074 reads in 179.338 CPU sec, 5.752 real sec
[M::process] read 677434 sequences (100000293 bp) in 2.104 CPU sec, 2.261 real sec...
sorting block 90, #reads = 1078209 mem = 536880975 peak = 1058279424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284412, 2, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (355.45, 164.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 577, 595)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1449)
[M::mem_pestat] mean and std.dev: (426.00, 263.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1876)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675614 reads in 101.133 CPU sec, 3.233 real sec
[M::process] read 683334 sequences (100000261 bp) in 2.048 CPU sec, 2.131 real sec...
[M::mem_process_seqs] Processed 676024 reads in 265.328 CPU sec, 8.504 real sec
sorting block 91, #reads = 1077658 mem = 536880937 peak = 1058279424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285306, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 320, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (344.15, 162.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682816 sequences (100000220 bp) in 2.867 CPU sec, 3.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287612, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 277, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (305.40, 146.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677434 reads in 212.371 CPU sec, 6.782 real sec
[M::mem_process_seqs] Processed 683334 reads in 125.653 CPU sec, 3.996 real sec
[M::process] read 681932 sequences (100000084 bp) in 2.250 CPU sec, 2.337 real sec...
sorting block 92, #reads = 1077004 mem = 536887444 peak = 1058279424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287199, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 280, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (307.87, 146.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681040 sequences (100000176 bp) in 2.968 CPU sec, 3.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286706, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 286, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (311.66, 146.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680204 sequences (100000180 bp) in 1.935 CPU sec, 1.955 real sec...
[M::mem_process_seqs] Processed 682816 reads in 200.308 CPU sec, 6.434 real sec
[M::mem_process_seqs] Processed 681932 reads in 127.562 CPU sec, 4.082 real sec
sorting block 93, #reads = 1079920 mem = 536878328 peak = 1058279424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287116, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 294, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (318.71, 148.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680098 sequences (100000269 bp) in 3.081 CPU sec, 3.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286565, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 300, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (323.31, 150.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679352 sequences (100000051 bp) in 1.961 CPU sec, 1.968 real sec...
[M::mem_process_seqs] Processed 681040 reads in 189.439 CPU sec, 6.053 real sec
sorting block 94, #reads = 1076829 mem = 536878822 peak = 1058279424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287139, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (253, 328, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (103, 478)
[M::mem_pestat] mean and std.dev: (250.25, 77.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 561)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 301, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (325.25, 152.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 679142 sequences (100000158 bp) in 2.386 CPU sec, 2.391 real sec...
[M::mem_process_seqs] Processed 680098 reads in 101.003 CPU sec, 3.186 real sec
sorting block 95, #reads = 1077585 mem = 536883721 peak = 1058279424
[M::mem_process_seqs] Processed 680204 reads in 247.354 CPU sec, 7.841 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286406, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 307, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (330.11, 153.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679222 sequences (100000150 bp) in 3.016 CPU sec, 3.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286857, 2, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 265, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584)
[M::mem_pestat] mean and std.dev: (237.10, 45.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 706)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 311, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (332.54, 154.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 679352 reads in 203.609 CPU sec, 6.499 real sec
sorting block 96, #reads = 1077960 mem = 536878188 peak = 1058279424
[M::mem_process_seqs] Processed 679142 reads in 122.298 CPU sec, 3.863 real sec
[M::process] read 678788 sequences (100000032 bp) in 2.131 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286657, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 310, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (331.41, 153.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 97, #reads = 1078414 mem = 536877669 peak = 1058279424
[M::process] read 678060 sequences (100000200 bp) in 2.338 CPU sec, 2.701 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286823, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (334.37, 154.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677936 sequences (100000145 bp) in 2.211 CPU sec, 2.247 real sec...
[M::mem_process_seqs] Processed 679222 reads in 194.338 CPU sec, 6.231 real sec
[M::mem_process_seqs] Processed 678788 reads in 127.971 CPU sec, 4.061 real sec
sorting block 98, #reads = 1078532 mem = 536882089 peak = 1058279424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285925, 14, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (339.21, 156.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4, 4, 1476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4420)
[M::mem_pestat] mean and std.dev: (605.86, 701.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5892)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 677510 sequences (100000287 bp) in 2.851 CPU sec, 3.155 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285901, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (340.97, 156.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677316 sequences (100000164 bp) in 1.821 CPU sec, 1.853 real sec...
[M::mem_process_seqs] Processed 678060 reads in 194.618 CPU sec, 6.237 real sec
[M::mem_process_seqs] Processed 677936 reads in 129.448 CPU sec, 4.128 real sec
sorting block 99, #reads = 1077946 mem = 536876957 peak = 1058279424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285216, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 322, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (343.04, 158.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (484, 484, 1745)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4267)
[M::mem_pestat] mean and std.dev: (835.40, 600.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5528)
[M::mem_pestat] skip orientation RR
[M::process] read 677084 sequences (100000134 bp) in 2.918 CPU sec, 3.271 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285349, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 324, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (344.77, 158.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677030 sequences (100000268 bp) in 2.035 CPU sec, 2.045 real sec...
[M::mem_process_seqs] Processed 677510 reads in 194.098 CPU sec, 6.207 real sec
[M::mem_process_seqs] Processed 677316 reads in 127.633 CPU sec, 4.054 real sec
sorting block 100, #reads = 1077885 mem = 536879068 peak = 1058279424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285534, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 324, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (345.52, 159.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676772 sequences (100000045 bp) in 2.862 CPU sec, 3.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285435, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 323, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (345.22, 159.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676596 sequences (100000086 bp) in 1.994 CPU sec, 2.005 real sec...
[M::mem_process_seqs] Processed 677084 reads in 187.829 CPU sec, 5.999 real sec
sorting block 101, #reads = 1077163 mem = 536882475 peak = 1058279424
[M::mem_process_seqs] Processed 677030 reads in 123.105 CPU sec, 3.922 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285484, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 326, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (346.95, 160.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 102, #reads = 1077444 mem = 536879651 peak = 1071874048
[M::process] read 676416 sequences (100000118 bp) in 3.169 CPU sec, 3.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284994, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (348.43, 160.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676772 reads in 189.903 CPU sec, 6.076 real sec
[M::process] read 676680 sequences (100000275 bp) in 2.055 CPU sec, 2.064 real sec...
[M::mem_process_seqs] Processed 676596 reads in 122.539 CPU sec, 3.902 real sec
sorting block 103, #reads = 1076918 mem = 536883438 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285247, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 327, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (348.50, 160.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676392 sequences (100000114 bp) in 2.903 CPU sec, 3.051 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285503, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (347.41, 159.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676068 sequences (100000030 bp) in 1.915 CPU sec, 1.916 real sec...
[M::mem_process_seqs] Processed 676416 reads in 173.647 CPU sec, 5.509 real sec
sorting block 104, #reads = 1078686 mem = 536879184 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284966, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (347.62, 160.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675852 sequences (100000018 bp) in 2.523 CPU sec, 2.526 real sec...
[M::mem_process_seqs] Processed 676392 reads in 96.192 CPU sec, 3.036 real sec
[M::mem_process_seqs] Processed 676680 reads in 242.327 CPU sec, 7.666 real sec
sorting block 105, #reads = 1078625 mem = 536880421 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285707, 2, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (276, 276, 998)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2442)
[M::mem_pestat] mean and std.dev: (606.50, 557.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3164)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (351.14, 161.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675878 sequences (100000136 bp) in 2.576 CPU sec, 2.717 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284808, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (351.66, 161.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 492, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (406.70, 131.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676068 reads in 183.416 CPU sec, 5.812 real sec
[M::mem_process_seqs] Processed 675852 reads in 108.207 CPU sec, 3.408 real sec
[M::process] read 676126 sequences (100000102 bp) in 2.298 CPU sec, 2.373 real sec...
sorting block 106, #reads = 1078760 mem = 536879621 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285406, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (352.46, 162.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676546 sequences (100000142 bp) in 2.890 CPU sec, 3.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284899, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (353.53, 165.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675878 reads in 167.656 CPU sec, 5.327 real sec
[M::mem_process_seqs] Processed 676126 reads in 109.324 CPU sec, 3.440 real sec
[M::process] read 682428 sequences (100000266 bp) in 2.037 CPU sec, 2.060 real sec...
sorting block 107, #reads = 1078853 mem = 536879930 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285143, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 328, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (351.65, 165.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681170 sequences (100000070 bp) in 2.433 CPU sec, 2.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286945, 5, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (619, 623, 1774)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4084)
[M::mem_pestat] mean and std.dev: (707.12, 431.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5239)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 285, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (311.95, 148.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 680494 sequences (100000198 bp) in 1.860 CPU sec, 1.858 real sec...
[M::mem_process_seqs] Processed 676546 reads in 179.726 CPU sec, 5.732 real sec
sorting block 108, #reads = 1077792 mem = 536878607 peak = 1071874048
[M::mem_process_seqs] Processed 682428 reads in 115.189 CPU sec, 3.664 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286543, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 294, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (319.07, 150.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 109, #reads = 1075363 mem = 536882342 peak = 1071874048
[M::process] read 679960 sequences (100000132 bp) in 3.156 CPU sec, 3.313 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286061, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 297, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (321.72, 151.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681170 reads in 161.605 CPU sec, 5.121 real sec
[M::mem_process_seqs] Processed 680494 reads in 100.381 CPU sec, 3.154 real sec
[M::process] read 679272 sequences (100000053 bp) in 2.340 CPU sec, 2.388 real sec...
sorting block 110, #reads = 1082604 mem = 536880577 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286474, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 302, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (324.66, 151.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678914 sequences (100000212 bp) in 2.417 CPU sec, 2.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286076, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 306, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (328.59, 152.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679960 reads in 169.276 CPU sec, 5.381 real sec
[M::process] read 678382 sequences (100000270 bp) in 1.984 CPU sec, 2.001 real sec...
[M::mem_process_seqs] Processed 679272 reads in 112.911 CPU sec, 3.560 real sec
sorting block 111, #reads = 1082364 mem = 536878697 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285848, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 308, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (329.37, 151.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678256 sequences (100000257 bp) in 3.175 CPU sec, 3.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285886, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (332.79, 152.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678914 reads in 176.496 CPU sec, 5.630 real sec
[M::mem_process_seqs] Processed 678382 reads in 111.856 CPU sec, 3.527 real sec
[M::process] read 678208 sequences (100000220 bp) in 2.258 CPU sec, 2.335 real sec...
sorting block 112, #reads = 1081337 mem = 536880226 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285164, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 315, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (334.70, 153.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678384 sequences (100000212 bp) in 2.694 CPU sec, 2.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286271, 4, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 317, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (336.60, 154.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 281, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (236.54, 78.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 678256 reads in 186.317 CPU sec, 5.952 real sec
sorting block 113, #reads = 1082222 mem = 536877576 peak = 1071874048
[M::process] read 678454 sequences (100000135 bp) in 2.123 CPU sec, 2.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285744, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 316, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (335.50, 153.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678384 reads in 100.054 CPU sec, 3.163 real sec
[M::process] read 678138 sequences (100000137 bp) in 2.365 CPU sec, 2.368 real sec...
sorting block 114, #reads = 1080592 mem = 536877691 peak = 1071874048
[M::mem_process_seqs] Processed 678208 reads in 256.228 CPU sec, 8.121 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285575, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 316, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (335.53, 153.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677778 sequences (100000276 bp) in 2.776 CPU sec, 2.927 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285363, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 316, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (335.05, 152.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 247, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (257.38, 49.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 678454 reads in 197.378 CPU sec, 6.265 real sec
[M::process] read 678146 sequences (100000031 bp) in 1.915 CPU sec, 1.936 real sec...
[M::mem_process_seqs] Processed 678138 reads in 115.978 CPU sec, 3.671 real sec
sorting block 115, #reads = 1080673 mem = 536879367 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285309, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 318, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (337.26, 153.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 116, #reads = 1080963 mem = 536878614 peak = 1071874048
[M::process] read 677948 sequences (100000061 bp) in 3.207 CPU sec, 3.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285670, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 313, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (333.35, 152.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677778 reads in 181.396 CPU sec, 5.773 real sec
[M::process] read 677034 sequences (100000174 bp) in 2.085 CPU sec, 2.106 real sec...
[M::mem_process_seqs] Processed 678146 reads in 116.482 CPU sec, 3.672 real sec
sorting block 117, #reads = 1080702 mem = 536879905 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285306, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 316, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (336.71, 154.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676934 sequences (100000144 bp) in 2.625 CPU sec, 2.752 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285246, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 325, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (343.73, 156.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677018 sequences (100000287 bp) in 1.982 CPU sec, 1.984 real sec...
[M::mem_process_seqs] Processed 677948 reads in 175.567 CPU sec, 5.582 real sec
[M::mem_process_seqs] Processed 677034 reads in 114.311 CPU sec, 3.610 real sec
sorting block 118, #reads = 1078716 mem = 536881007 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285307, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 325, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (344.26, 156.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676626 sequences (100000255 bp) in 2.626 CPU sec, 2.741 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284914, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 326, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (345.10, 157.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677040 sequences (100000194 bp) in 1.700 CPU sec, 1.693 real sec...
[M::mem_process_seqs] Processed 676934 reads in 166.236 CPU sec, 5.280 real sec
[M::mem_process_seqs] Processed 677018 reads in 107.578 CPU sec, 3.402 real sec
sorting block 119, #reads = 1078786 mem = 536878621 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284892, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 327, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (347.45, 158.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677022 sequences (100000110 bp) in 2.444 CPU sec, 2.583 real sec...
[M::process] read 676608 sequences (100000162 bp) in 1.540 CPU sec, 1.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285172, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (343.03, 157.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676626 reads in 163.712 CPU sec, 5.203 real sec
sorting block 120, #reads = 1077854 mem = 536877406 peak = 1071874048
[M::mem_process_seqs] Processed 677040 reads in 105.380 CPU sec, 3.341 real sec
sorting block 121, #reads = 1077486 mem = 536880765 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285520, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 320, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (340.64, 155.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676458 sequences (100000059 bp) in 2.536 CPU sec, 2.649 real sec...
[M::process] read 676658 sequences (100000290 bp) in 1.660 CPU sec, 1.662 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285100, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 324, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (343.82, 156.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677022 reads in 165.530 CPU sec, 5.251 real sec
[M::mem_process_seqs] Processed 676608 reads in 104.815 CPU sec, 3.325 real sec
sorting block 122, #reads = 1076620 mem = 536877060 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284722, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (347.14, 159.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676602 sequences (100000024 bp) in 2.656 CPU sec, 2.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285372, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 325, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (345.97, 159.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676450 sequences (100000217 bp) in 1.780 CPU sec, 1.779 real sec...
[M::mem_process_seqs] Processed 676458 reads in 174.433 CPU sec, 5.597 real sec
[M::mem_process_seqs] Processed 676658 reads in 109.296 CPU sec, 3.472 real sec
sorting block 123, #reads = 1077889 mem = 536881488 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285418, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (347.50, 159.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676606 sequences (100000042 bp) in 2.572 CPU sec, 2.696 real sec...
[M::process] read 681494 sequences (100000030 bp) in 1.610 CPU sec, 1.604 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285656, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (348.06, 159.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676602 reads in 174.383 CPU sec, 5.582 real sec
sorting block 124, #reads = 1076896 mem = 536881810 peak = 1071874048
[M::mem_process_seqs] Processed 676450 reads in 113.665 CPU sec, 3.636 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284888, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 325, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (347.66, 161.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683996 sequences (100000002 bp) in 2.354 CPU sec, 2.462 real sec...
[M::process] read 682696 sequences (100000030 bp) in 1.830 CPU sec, 1.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286651, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 292, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (319.20, 152.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676606 reads in 188.012 CPU sec, 5.997 real sec
sorting block 125, #reads = 1075438 mem = 536880138 peak = 1071874048
[M::mem_process_seqs] Processed 681494 reads in 124.473 CPU sec, 3.950 real sec
sorting block 126, #reads = 1067925 mem = 536877633 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288185, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 276, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (302.34, 143.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682194 sequences (100000194 bp) in 3.131 CPU sec, 3.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287422, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 284, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (309.47, 145.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680706 sequences (100000170 bp) in 2.070 CPU sec, 2.069 real sec...
[M::mem_process_seqs] Processed 683996 reads in 182.459 CPU sec, 5.786 real sec
[M::mem_process_seqs] Processed 682696 reads in 121.336 CPU sec, 3.840 real sec
sorting block 127, #reads = 1078104 mem = 536881305 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287262, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 289, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (312.03, 144.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680364 sequences (100000215 bp) in 2.910 CPU sec, 3.046 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287179, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 297, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (317.98, 145.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679736 sequences (100000229 bp) in 2.238 CPU sec, 2.245 real sec...
[M::mem_process_seqs] Processed 682194 reads in 191.603 CPU sec, 6.087 real sec
[M::mem_process_seqs] Processed 680706 reads in 124.888 CPU sec, 3.947 real sec
sorting block 128, #reads = 1078101 mem = 536878494 peak = 1071874048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286980, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 300, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (321.43, 147.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679682 sequences (100000293 bp) in 2.943 CPU sec, 3.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286890, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 304, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (325.54, 149.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678904 sequences (100000139 bp) in 2.193 CPU sec, 2.198 real sec...
[M::mem_process_seqs] Processed 680364 reads in 188.394 CPU sec, 5.976 real sec
sorting block 129, #reads = 1076908 mem = 536879836 peak = 1071874048
[M::mem_process_seqs] Processed 679736 reads in 121.694 CPU sec, 3.851 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286415, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 307, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (327.19, 149.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 130, #reads = 1077253 mem = 536878589 peak = 1071874048
[M::process] read 679380 sequences (100000108 bp) in 2.762 CPU sec, 2.900 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285958, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 313, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (332.90, 153.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678852 sequences (100000071 bp) in 2.220 CPU sec, 2.216 real sec...
[M::mem_process_seqs] Processed 679682 reads in 183.299 CPU sec, 5.819 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285997, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 309, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (330.02, 151.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678422 sequences (100000125 bp) in 2.554 CPU sec, 2.556 real sec...
[M::mem_process_seqs] Processed 679380 reads in 107.360 CPU sec, 3.393 real sec
sorting block 131, #reads = 1075873 mem = 536880395 peak = 1071874048
[M::mem_process_seqs] Processed 678904 reads in 261.858 CPU sec, 8.299 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286167, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 315, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (335.18, 154.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679672 sequences (100000150 bp) in 3.027 CPU sec, 3.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286147, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 317, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (336.48, 154.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678852 reads in 204.563 CPU sec, 6.501 real sec
sorting block 132, #reads = 1076009 mem = 536880382 peak = 1071874048
[M::mem_process_seqs] Processed 678422 reads in 120.801 CPU sec, 3.811 real sec
[M::process] read 677948 sequences (100000201 bp) in 2.513 CPU sec, 2.609 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286446, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 303, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (326.89, 152.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 133, #reads = 1076787 mem = 536877445 peak = 1088061440
[M::process] read 677472 sequences (100000033 bp) in 2.955 CPU sec, 3.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285564, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 314, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (334.01, 151.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679672 reads in 185.863 CPU sec, 5.914 real sec
[M::process] read 676964 sequences (100000026 bp) in 1.993 CPU sec, 2.006 real sec...
[M::mem_process_seqs] Processed 677948 reads in 120.806 CPU sec, 3.818 real sec
sorting block 134, #reads = 1077006 mem = 536877859 peak = 1088061440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285371, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 318, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (337.25, 152.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676880 sequences (100000144 bp) in 3.055 CPU sec, 3.227 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285398, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 322, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (340.26, 153.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677472 reads in 183.772 CPU sec, 5.848 real sec
[M::process] read 676974 sequences (100000182 bp) in 2.208 CPU sec, 2.235 real sec...
[M::mem_process_seqs] Processed 676964 reads in 118.072 CPU sec, 3.721 real sec
sorting block 135, #reads = 1076445 mem = 536878755 peak = 1088061440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284893, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 321, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (339.97, 152.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677024 sequences (100000296 bp) in 3.025 CPU sec, 3.185 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285373, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 319, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (338.79, 152.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676880 reads in 185.994 CPU sec, 5.914 real sec
[M::process] read 676556 sequences (100000097 bp) in 2.069 CPU sec, 2.073 real sec...
sorting block 136, #reads = 1077289 mem = 536878098 peak = 1088061440
[M::mem_process_seqs] Processed 676974 reads in 119.725 CPU sec, 3.779 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285193, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 316, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (336.62, 151.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676446 sequences (100000253 bp) in 2.736 CPU sec, 2.885 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285157, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 316, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869)
[M::mem_pestat] mean and std.dev: (336.08, 150.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677024 reads in 179.871 CPU sec, 5.711 real sec
[M::process] read 676148 sequences (100000038 bp) in 2.150 CPU sec, 2.149 real sec...
sorting block 137, #reads = 1078188 mem = 536879226 peak = 1088061440
[M::mem_process_seqs] Processed 676556 reads in 115.753 CPU sec, 3.651 real sec
sorting block 138, #reads = 1078635 mem = 536877625 peak = 1120714752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284940, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 320, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (339.81, 152.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676128 sequences (100000190 bp) in 3.148 CPU sec, 3.312 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284790, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (289, 890, 901)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2125)
[M::mem_pestat] mean and std.dev: (708.36, 299.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2737)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 319, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (340.17, 154.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676446 reads in 178.197 CPU sec, 5.643 real sec
[M::process] read 675798 sequences (100000291 bp) in 2.057 CPU sec, 2.059 real sec...
[M::mem_process_seqs] Processed 676148 reads in 112.879 CPU sec, 3.557 real sec
sorting block 139, #reads = 1078520 mem = 536879566 peak = 1120714752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285030, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (347.55, 159.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675620 sequences (100000061 bp) in 2.662 CPU sec, 2.822 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284909, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (350.75, 160.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676128 reads in 172.654 CPU sec, 5.498 real sec
[M::process] read 675618 sequences (100000061 bp) in 2.041 CPU sec, 2.057 real sec...
[M::mem_process_seqs] Processed 675798 reads in 110.279 CPU sec, 3.478 real sec
sorting block 140, #reads = 1078973 mem = 536879628 peak = 1120714752
[M::process] read 496816 sequences (73482843 bp) in 2.618 CPU sec, 2.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284238, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (356.18, 164.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675618 reads in 98.277 CPU sec, 3.161 real sec
sorting block 141, #reads = 1078388 mem = 536879603 peak = 1120714752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284763, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (350.59, 160.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 209852, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (346, 346, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (346, 346)
[M::mem_pestat] mean and std.dev: (346.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (346, 346)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (351.81, 162.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675620 reads in 242.466 CPU sec, 7.821 real sec
[M::mem_process_seqs] Processed 496816 reads in 104.604 CPU sec, 3.400 real sec
sorting block 142, #reads = 1078919 mem = 536879696 peak = 1120714752
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 154216210
overall: 69725036544 mem 23512.028 user 530.617 sys 786.965 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_d0M-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:659_d0M-T1-TDNA-1_A23MHWWLT4_1\tLB:659_d0M-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_d0M-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_d0M-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 787.022 sec; CPU: 24042.667 sec
sorting block 143, #reads = 535647 mem = 267027304 peak = 1120714752
uniq header count 1
merging 144 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8413137430
output reads: 154973051
bam_mem_sort: 144 calls 90.635 user 1.740 sys 94.770 real
bam_write: 144 calls 79.151 user 4.839 sys 150.409 real
execute: 1 calls 43.646 user 2.782 sys 846.009 real
merge_files: 1 calls 43.631 user 2.752 sys 58.504 real
parse_chunk: 2296 calls 105.363 user 24.515 sys 157.552 real
read_chunk: 6186 calls 54.605 user 34.911 sys 744.038 real
sort_block: 1 calls 34.225 user 34.184 sys 716.908 real
write_chunk: 3888 calls 43.143 user 2.433 sys 57.429 real
overall: 3167346688 mem 2274.094 user 92.859 sys 846.032 real
ls: cannot access '*.cram': No such file or directory