Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a5/ea5bb7e031c1402088ef9a136bee66/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/40/92dc86375574e466d7d9389e0d6719/659_cAd-T1-TDNA-1_A23MHWWLT4_1/659_cAd-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a5/ea5bb7e031c1402088ef9a136bee66/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/40/92dc86375574e466d7d9389e0d6719/659_cAd-T1-TDNA-1_A23MHWWLT4_1/659_cAd-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_cAd-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1861.248 CPU sec, 67.606 real sec
[M::process] read 686628 sequences (100000128 bp) in 1.353 CPU sec, 1.362 real sec...
[M::process] read 685338 sequences (100000112 bp) in 1.430 CPU sec, 1.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288050, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 279, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (295.29, 136.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684486 sequences (100000249 bp) in 1.734 CPU sec, 1.742 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287619, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (300.38, 138.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684030 sequences (100000135 bp) in 1.752 CPU sec, 1.763 real sec...
[M::mem_process_seqs] Processed 686628 reads in 174.576 CPU sec, 5.486 real sec
[M::mem_process_seqs] Processed 685338 reads in 117.521 CPU sec, 3.737 real sec
sorting block 0, #reads = 1087239 mem = 536878703 peak = 588144640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287080, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (305.49, 139.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683868 sequences (100000100 bp) in 2.475 CPU sec, 2.597 real sec...
[M::process] read 683694 sequences (100000063 bp) in 1.894 CPU sec, 1.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286828, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 294, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (308.55, 140.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684486 reads in 172.315 CPU sec, 5.497 real sec
[M::mem_process_seqs] Processed 684030 reads in 116.400 CPU sec, 3.731 real sec
sorting block 1, #reads = 1085996 mem = 536881948 peak = 713330688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286888, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 298, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (311.75, 142.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683544 sequences (100000124 bp) in 2.337 CPU sec, 2.733 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286855, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 298, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (311.31, 142.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683868 reads in 169.973 CPU sec, 5.440 real sec
[M::process] read 683062 sequences (100000115 bp) in 2.033 CPU sec, 2.033 real sec...
[M::mem_process_seqs] Processed 683694 reads in 116.375 CPU sec, 3.679 real sec
sorting block 2, #reads = 1086197 mem = 536879798 peak = 835538944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287083, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 301, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (314.11, 143.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682812 sequences (100000244 bp) in 2.971 CPU sec, 3.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287193, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 303, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (316.25, 144.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683544 reads in 171.024 CPU sec, 5.569 real sec
[M::mem_process_seqs] Processed 683062 reads in 118.544 CPU sec, 3.930 real sec
sorting block 3, #reads = 1085969 mem = 536880030 peak = 837529600
[M::process] read 682276 sequences (100000108 bp) in 2.537 CPU sec, 2.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287008, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (316.76, 144.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1085604 mem = 536877340 peak = 896249856
[M::process] read 682228 sequences (100000150 bp) in 2.788 CPU sec, 3.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286867, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 305, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (317.98, 143.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682812 reads in 173.220 CPU sec, 5.605 real sec
[M::mem_process_seqs] Processed 682276 reads in 118.980 CPU sec, 3.785 real sec
[M::process] read 681856 sequences (100000040 bp) in 2.544 CPU sec, 2.742 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286749, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 307, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (319.65, 144.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 5, #reads = 1086338 mem = 536879492 peak = 896249856
[M::process] read 681562 sequences (100000025 bp) in 3.056 CPU sec, 3.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286905, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 307, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (320.79, 145.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682228 reads in 171.071 CPU sec, 5.516 real sec
[M::mem_process_seqs] Processed 681856 reads in 116.265 CPU sec, 3.709 real sec
[M::process] read 681802 sequences (100000224 bp) in 2.934 CPU sec, 3.168 real sec...
sorting block 6, #reads = 1085804 mem = 536880998 peak = 896249856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286829, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 309, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (321.74, 145.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286642, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 210, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687)
[M::mem_pestat] mean and std.dev: (259.09, 95.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 309, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (321.79, 145.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681540 sequences (100000098 bp) in 2.879 CPU sec, 3.249 real sec...
[M::mem_process_seqs] Processed 681562 reads in 178.280 CPU sec, 5.884 real sec
[M::mem_process_seqs] Processed 681802 reads in 121.846 CPU sec, 4.024 real sec
sorting block 7, #reads = 1086790 mem = 536882250 peak = 896249856
[M::process] read 681306 sequences (100000143 bp) in 2.959 CPU sec, 3.192 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286793, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 309, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (322.96, 146.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (950, 950, 3573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8819)
[M::mem_pestat] mean and std.dev: (1736.90, 1202.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11442)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286622, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 310, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (322.83, 144.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 1644, 1775)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5047)
[M::mem_pestat] mean and std.dev: (1715.00, 1742.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8684)
[M::mem_pestat] skip orientation RR
[M::process] read 680984 sequences (100000033 bp) in 2.731 CPU sec, 3.132 real sec...
[M::mem_process_seqs] Processed 681540 reads in 178.498 CPU sec, 5.981 real sec
[M::mem_process_seqs] Processed 681306 reads in 122.326 CPU sec, 4.056 real sec
sorting block 8, #reads = 1085932 mem = 536878166 peak = 896249856
[M::process] read 680936 sequences (100000144 bp) in 2.952 CPU sec, 3.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286367, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 311, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (323.11, 144.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 9, #reads = 1084621 mem = 536879172 peak = 896249856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286636, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (323.37, 144.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (107, 213, 868)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2390)
[M::mem_pestat] mean and std.dev: (521.30, 508.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3151)
[M::mem_pestat] skip orientation RR
[M::process] read 680544 sequences (100000232 bp) in 2.886 CPU sec, 3.228 real sec...
[M::mem_process_seqs] Processed 680984 reads in 181.197 CPU sec, 6.054 real sec
[M::mem_process_seqs] Processed 680936 reads in 122.085 CPU sec, 4.081 real sec
[M::process] read 680394 sequences (100000013 bp) in 2.939 CPU sec, 3.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286048, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 310, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (321.21, 141.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 10, #reads = 1085204 mem = 536877912 peak = 896249856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286085, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 312, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (323.20, 142.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680170 sequences (100000068 bp) in 2.963 CPU sec, 3.338 real sec...
[M::mem_process_seqs] Processed 680544 reads in 181.654 CPU sec, 6.042 real sec
[M::mem_process_seqs] Processed 680394 reads in 122.745 CPU sec, 4.013 real sec
sorting block 11, #reads = 1084154 mem = 536882099 peak = 896249856
[M::process] read 680682 sequences (100000284 bp) in 3.185 CPU sec, 3.469 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286752, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 312, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (324.15, 143.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286772, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 309, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (322.01, 143.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680568 sequences (100000101 bp) in 2.733 CPU sec, 3.244 real sec...
[M::mem_process_seqs] Processed 680170 reads in 185.402 CPU sec, 6.260 real sec
[M::mem_process_seqs] Processed 680682 reads in 125.707 CPU sec, 4.218 real sec
sorting block 12, #reads = 1084018 mem = 536876851 peak = 896249856
[M::process] read 680870 sequences (100000031 bp) in 3.195 CPU sec, 3.590 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286196, 10, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 305, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (317.50, 139.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (454, 454, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (454, 454)
[M::mem_pestat] mean and std.dev: (454.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (454, 454)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286884, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 305, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (317.31, 140.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680752 sequences (100000206 bp) in 3.007 CPU sec, 3.303 real sec...
[M::mem_process_seqs] Processed 680568 reads in 185.474 CPU sec, 6.199 real sec
[M::mem_process_seqs] Processed 680870 reads in 123.875 CPU sec, 4.085 real sec
sorting block 13, #reads = 1083474 mem = 536877874 peak = 896249856
[M::process] read 680256 sequences (100000034 bp) in 2.960 CPU sec, 3.216 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285839, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 305, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (317.28, 140.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 14, #reads = 1082728 mem = 536878311 peak = 909266944
[M::process] read 680570 sequences (100000224 bp) in 2.828 CPU sec, 3.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286968, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 312, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (323.50, 143.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680752 reads in 188.584 CPU sec, 6.372 real sec
[M::mem_process_seqs] Processed 680256 reads in 126.212 CPU sec, 4.234 real sec
[M::process] read 680344 sequences (100000207 bp) in 2.838 CPU sec, 3.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286792, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 311, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (323.70, 144.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 15, #reads = 1081668 mem = 536881329 peak = 909266944
[M::process] read 680444 sequences (100000101 bp) in 2.885 CPU sec, 3.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286391, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 310, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (323.34, 144.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680570 reads in 184.443 CPU sec, 6.118 real sec
[M::mem_process_seqs] Processed 680344 reads in 126.691 CPU sec, 4.160 real sec
[M::process] read 685618 sequences (100000265 bp) in 3.068 CPU sec, 3.332 real sec...
sorting block 16, #reads = 1082364 mem = 536879102 peak = 909266944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286666, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (326.05, 147.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685180 sequences (100000137 bp) in 2.983 CPU sec, 3.265 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287952, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 286, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (302.74, 141.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680444 reads in 190.756 CPU sec, 6.311 real sec
[M::mem_process_seqs] Processed 685618 reads in 128.040 CPU sec, 4.156 real sec
sorting block 17, #reads = 1081144 mem = 536880015 peak = 909266944
[M::process] read 684106 sequences (100000145 bp) in 2.783 CPU sec, 3.013 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287687, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 287, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (303.55, 141.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287468, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 294, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (309.30, 142.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683892 sequences (100000156 bp) in 2.907 CPU sec, 3.524 real sec...
[M::mem_process_seqs] Processed 685180 reads in 181.844 CPU sec, 6.166 real sec
sorting block 18, #reads = 1084797 mem = 536877765 peak = 909266944
[M::mem_process_seqs] Processed 684106 reads in 119.305 CPU sec, 3.954 real sec
[M::process] read 683920 sequences (100000133 bp) in 2.968 CPU sec, 3.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287393, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 295, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (309.63, 141.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287226, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (246, 271, 641)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1431)
[M::mem_pestat] mean and std.dev: (389.36, 192.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1826)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 296, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (310.03, 141.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 683892 reads in 173.094 CPU sec, 5.753 real sec
sorting block 19, #reads = 1088180 mem = 536878611 peak = 909266944
[M::process] read 683810 sequences (100000201 bp) in 3.044 CPU sec, 3.415 real sec...
[M::mem_process_seqs] Processed 683920 reads in 118.914 CPU sec, 3.944 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287168, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 297, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (311.02, 141.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683310 sequences (100000213 bp) in 2.854 CPU sec, 3.079 real sec...
[M::mem_process_seqs] Processed 683810 reads in 97.687 CPU sec, 3.310 real sec
sorting block 20, #reads = 1087727 mem = 536878669 peak = 909266944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286986, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 298, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (312.14, 141.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682926 sequences (100000160 bp) in 2.773 CPU sec, 3.114 real sec...
[M::mem_process_seqs] Processed 683310 reads in 97.037 CPU sec, 3.345 real sec
sorting block 21, #reads = 1087070 mem = 536878718 peak = 909266944
[M::process] read 682848 sequences (100000113 bp) in 2.671 CPU sec, 2.814 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287724, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 304, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (317.43, 145.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287431, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 303, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (316.33, 144.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682584 sequences (100000064 bp) in 2.609 CPU sec, 2.879 real sec...
[M::mem_process_seqs] Processed 682926 reads in 173.543 CPU sec, 5.699 real sec
[M::mem_process_seqs] Processed 682848 reads in 118.259 CPU sec, 3.864 real sec
sorting block 22, #reads = 1086205 mem = 536876110 peak = 909266944
[M::process] read 682260 sequences (100000004 bp) in 2.809 CPU sec, 3.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287256, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 303, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (315.82, 143.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286886, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (316.78, 142.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682132 sequences (100000187 bp) in 2.702 CPU sec, 3.100 real sec...
[M::mem_process_seqs] Processed 682584 reads in 170.938 CPU sec, 5.541 real sec
sorting block 23, #reads = 1086547 mem = 536878441 peak = 909266944
[M::mem_process_seqs] Processed 682260 reads in 115.932 CPU sec, 3.783 real sec
[M::process] read 681956 sequences (100000070 bp) in 2.642 CPU sec, 2.979 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286741, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 306, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (318.53, 143.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 159, 178)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (13, 288)
[M::mem_pestat] mean and std.dev: (141.25, 24.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 343)
[M::mem_pestat] skip orientation RR
sorting block 24, #reads = 1087186 mem = 536879674 peak = 909266944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286949, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 306, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (318.73, 143.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (298, 551, 551)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057)
[M::mem_pestat] mean and std.dev: (435.33, 129.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] skip orientation RR
[M::process] read 681792 sequences (100000180 bp) in 2.715 CPU sec, 2.990 real sec...
[M::mem_process_seqs] Processed 682132 reads in 173.664 CPU sec, 5.680 real sec
[M::mem_process_seqs] Processed 681956 reads in 117.900 CPU sec, 3.784 real sec
[M::process] read 681764 sequences (100000005 bp) in 2.769 CPU sec, 2.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287072, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 307, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (320.63, 145.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1086270 mem = 536877630 peak = 909266944
[M::process] read 681462 sequences (100000124 bp) in 2.635 CPU sec, 3.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286758, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 307, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (320.68, 144.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681792 reads in 178.685 CPU sec, 5.900 real sec
[M::mem_process_seqs] Processed 681764 reads in 121.084 CPU sec, 3.957 real sec
sorting block 26, #reads = 1085198 mem = 536881405 peak = 909266944
[M::process] read 681556 sequences (100000023 bp) in 2.745 CPU sec, 2.910 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286785, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (321.14, 145.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286702, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (320.81, 144.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681570 sequences (100000223 bp) in 2.706 CPU sec, 3.244 real sec...
[M::mem_process_seqs] Processed 681462 reads in 168.436 CPU sec, 5.546 real sec
[M::mem_process_seqs] Processed 681556 reads in 116.876 CPU sec, 3.861 real sec
sorting block 27, #reads = 1085575 mem = 536880271 peak = 909266944
[M::process] read 681088 sequences (100000047 bp) in 2.673 CPU sec, 2.949 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286878, 10, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 308, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (320.68, 143.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (54, 113, 767)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2193)
[M::mem_pestat] mean and std.dev: (229.22, 289.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2906)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 28, #reads = 1084467 mem = 536883501 peak = 943370240
[M::process] read 681070 sequences (100000081 bp) in 2.444 CPU sec, 2.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287001, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 310, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (321.85, 143.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681570 reads in 170.954 CPU sec, 5.519 real sec
[M::mem_process_seqs] Processed 681088 reads in 117.648 CPU sec, 3.800 real sec
[M::process] read 680922 sequences (100000141 bp) in 2.297 CPU sec, 2.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287056, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 308, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (320.84, 143.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 29, #reads = 1084108 mem = 536877790 peak = 943370240
[M::process] read 681286 sequences (100000121 bp) in 2.494 CPU sec, 2.867 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286579, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 307, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (320.03, 143.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681070 reads in 167.056 CPU sec, 5.440 real sec
[M::mem_process_seqs] Processed 680922 reads in 115.021 CPU sec, 3.683 real sec
[M::process] read 681488 sequences (100000133 bp) in 2.460 CPU sec, 2.555 real sec...
sorting block 30, #reads = 1083778 mem = 536878517 peak = 943370240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286864, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 305, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (318.14, 142.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680844 sequences (100000122 bp) in 2.468 CPU sec, 2.837 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287191, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 305, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (319.00, 143.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681286 reads in 169.156 CPU sec, 5.474 real sec
[M::mem_process_seqs] Processed 681488 reads in 116.473 CPU sec, 3.738 real sec
[M::process] read 680838 sequences (100000137 bp) in 2.298 CPU sec, 2.350 real sec...
sorting block 31, #reads = 1084091 mem = 536879936 peak = 943370240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286055, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 304, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (317.50, 141.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680760 sequences (100000141 bp) in 2.545 CPU sec, 2.741 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285921, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 306, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (318.83, 141.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680844 reads in 167.827 CPU sec, 5.439 real sec
[M::process] read 680778 sequences (100000231 bp) in 2.145 CPU sec, 2.192 real sec...
[M::mem_process_seqs] Processed 680838 reads in 115.402 CPU sec, 3.721 real sec
sorting block 32, #reads = 1084208 mem = 536879464 peak = 946130944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286689, 7, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 286, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 434)
[M::mem_pestat] mean and std.dev: (252.36, 76.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 557)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 308, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (320.78, 143.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 33, #reads = 1083174 mem = 536878376 peak = 946130944
[M::process] read 680720 sequences (100000103 bp) in 2.823 CPU sec, 3.127 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286263, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 309, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (322.53, 144.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680760 reads in 168.619 CPU sec, 5.442 real sec
[M::mem_process_seqs] Processed 680778 reads in 116.962 CPU sec, 3.738 real sec
[M::process] read 680558 sequences (100000028 bp) in 2.307 CPU sec, 2.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286097, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (540, 540, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (540, 540)
[M::mem_pestat] mean and std.dev: (540.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (540, 540)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 310, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (323.70, 146.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 34, #reads = 1082873 mem = 536879102 peak = 946130944
[M::process] read 684672 sequences (100000135 bp) in 2.438 CPU sec, 2.747 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286526, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 311, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (324.16, 145.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680720 reads in 167.556 CPU sec, 5.444 real sec
[M::mem_process_seqs] Processed 680558 reads in 115.561 CPU sec, 3.691 real sec
[M::process] read 685784 sequences (100000064 bp) in 2.487 CPU sec, 2.622 real sec...
sorting block 35, #reads = 1083626 mem = 536877815 peak = 946130944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287568, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 288, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (304.57, 140.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684128 sequences (100000081 bp) in 2.444 CPU sec, 2.947 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 288018, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (298.97, 138.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684672 reads in 170.336 CPU sec, 5.549 real sec
[M::mem_process_seqs] Processed 685784 reads in 117.333 CPU sec, 3.790 real sec
[M::process] read 683768 sequences (100000172 bp) in 2.415 CPU sec, 2.462 real sec...
sorting block 36, #reads = 1084409 mem = 536882974 peak = 957374464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287360, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 293, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (307.94, 141.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683188 sequences (100000037 bp) in 2.360 CPU sec, 2.773 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286843, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 295, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (309.79, 142.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684128 reads in 166.017 CPU sec, 5.342 real sec
sorting block 37, #reads = 1087405 mem = 536880271 peak = 998154240
[M::mem_process_seqs] Processed 683768 reads in 115.376 CPU sec, 3.666 real sec
[M::process] read 683008 sequences (100000111 bp) in 2.753 CPU sec, 2.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 286778, 13, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 373, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1273)
[M::mem_pestat] mean and std.dev: (239.50, 145.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1653)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 298, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (312.02, 141.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (33, 110, 2928)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8718)
[M::mem_pestat] mean and std.dev: (1099.23, 1394.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11613)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
sorting block 38, #reads = 1086467 mem = 536881112 peak = 998154240
[M::process] read 682756 sequences (100000117 bp) in 2.718 CPU sec, 3.046 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287109, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 301, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (314.62, 143.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683188 reads in 166.158 CPU sec, 5.410 real sec
[M::mem_process_seqs] Processed 683008 reads in 115.554 CPU sec, 3.693 real sec
[M::process] read 682372 sequences (100000143 bp) in 2.537 CPU sec, 2.684 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 287491, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (366, 4556, 4556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12936)
[M::mem_pestat] mean and std.dev: (2936.44, 1988.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17126)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (317.27, 145.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 39, #reads = 1085102 mem = 536880584 peak = 998154240
[M::process] read 682328 sequences (100000244 bp) in 2.449 CPU sec, 2.736 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286719, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (318.56, 145.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682756 reads in 162.356 CPU sec, 5.262 real sec
[M::mem_process_seqs] Processed 682372 reads in 111.767 CPU sec, 3.599 real sec
[M::process] read 682240 sequences (100000040 bp) in 2.588 CPU sec, 2.782 real sec...
sorting block 40, #reads = 1085627 mem = 536879145 peak = 998154240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286786, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 306, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (320.15, 146.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681834 sequences (100000097 bp) in 2.522 CPU sec, 2.856 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286702, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 307, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (321.70, 147.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (702, 3272, 3272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8412)
[M::mem_pestat] mean and std.dev: (1915.17, 1379.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10982)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682328 reads in 163.654 CPU sec, 5.311 real sec
[M::mem_process_seqs] Processed 682240 reads in 114.131 CPU sec, 3.670 real sec
sorting block 41, #reads = 1085311 mem = 536880331 peak = 998154240
[M::process] read 681522 sequences (100000293 bp) in 2.656 CPU sec, 2.734 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287158, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 308, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (322.35, 147.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682014 sequences (100000146 bp) in 2.476 CPU sec, 2.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286715, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 310, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (323.41, 146.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681834 reads in 161.773 CPU sec, 5.220 real sec
sorting block 42, #reads = 1084788 mem = 536879351 peak = 998154240
[M::mem_process_seqs] Processed 681522 reads in 111.840 CPU sec, 3.566 real sec
[M::process] read 682136 sequences (100000127 bp) in 2.692 CPU sec, 2.813 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287242, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 306, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (321.25, 147.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 43, #reads = 1084978 mem = 536878840 peak = 1020424192
[M::process] read 681412 sequences (100000066 bp) in 2.380 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287094, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (319.13, 146.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682014 reads in 162.072 CPU sec, 5.265 real sec
[M::mem_process_seqs] Processed 682136 reads in 112.036 CPU sec, 3.632 real sec
[M::process] read 681198 sequences (100000043 bp) in 2.280 CPU sec, 2.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286831, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 310, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (324.23, 147.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 44, #reads = 1086741 mem = 536878520 peak = 1020424192
[M::process] read 681078 sequences (100000116 bp) in 2.406 CPU sec, 2.942 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286619, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 310, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (322.92, 145.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681412 reads in 163.029 CPU sec, 5.321 real sec
[M::mem_process_seqs] Processed 681198 reads in 112.133 CPU sec, 3.625 real sec
[M::process] read 680770 sequences (100000274 bp) in 2.377 CPU sec, 2.441 real sec...
sorting block 45, #reads = 1085820 mem = 536879067 peak = 1020424192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286633, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 310, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (323.07, 145.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680696 sequences (100000269 bp) in 2.480 CPU sec, 2.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287081, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 311, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (324.67, 146.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681078 reads in 163.321 CPU sec, 5.259 real sec
[M::mem_process_seqs] Processed 680770 reads in 113.359 CPU sec, 3.600 real sec
sorting block 46, #reads = 1084543 mem = 536880242 peak = 1020424192
[M::process] read 680952 sequences (100000296 bp) in 2.395 CPU sec, 2.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286468, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 308, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (322.25, 145.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681116 sequences (100000081 bp) in 2.488 CPU sec, 2.673 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286451, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 307, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (321.22, 145.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680696 reads in 164.387 CPU sec, 5.295 real sec
sorting block 47, #reads = 1085644 mem = 536878204 peak = 1020424192
[M::mem_process_seqs] Processed 680952 reads in 112.373 CPU sec, 3.580 real sec
[M::process] read 681358 sequences (100000089 bp) in 2.338 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286330, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 306, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (319.98, 144.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (318, 336, 2106)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5682)
[M::mem_pestat] mean and std.dev: (860.33, 1002.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7470)
[M::mem_pestat] skip orientation RR
sorting block 48, #reads = 1083910 mem = 536877704 peak = 1072640000
[M::process] read 681392 sequences (100000027 bp) in 2.554 CPU sec, 2.827 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286640, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 304, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (318.44, 144.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681116 reads in 164.629 CPU sec, 5.341 real sec
[M::mem_process_seqs] Processed 681358 reads in 114.964 CPU sec, 3.705 real sec
[M::process] read 681518 sequences (100000084 bp) in 2.359 CPU sec, 2.450 real sec...
sorting block 49, #reads = 1084281 mem = 536878679 peak = 1072640000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287360, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (319.53, 145.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680698 sequences (100000138 bp) in 2.493 CPU sec, 2.921 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287434, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 307, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (320.45, 145.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681392 reads in 165.894 CPU sec, 5.361 real sec
[M::mem_process_seqs] Processed 681518 reads in 114.618 CPU sec, 3.668 real sec
[M::process] read 680698 sequences (100000109 bp) in 2.355 CPU sec, 2.405 real sec...
sorting block 50, #reads = 1083927 mem = 536877829 peak = 1072640000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286164, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 308, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (321.10, 144.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680550 sequences (100000132 bp) in 2.462 CPU sec, 2.810 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286355, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 309, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (322.49, 144.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680698 reads in 166.015 CPU sec, 5.347 real sec
[M::mem_process_seqs] Processed 680698 reads in 115.662 CPU sec, 3.677 real sec
sorting block 51, #reads = 1084372 mem = 536880987 peak = 1072640000
[M::process] read 680652 sequences (100000038 bp) in 2.335 CPU sec, 2.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286300, 0, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (275, 282, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (87, 557)
[M::mem_pestat] mean and std.dev: (287.10, 49.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 651)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 311, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (324.92, 146.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 324, 1187)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3063)
[M::mem_pestat] mean and std.dev: (495.11, 406.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4001)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680934 sequences (100000009 bp) in 2.604 CPU sec, 3.060 real sec...
sorting block 52, #reads = 1082217 mem = 536877628 peak = 1072640000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287091, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 8059, 8059)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23735)
[M::mem_pestat] mean and std.dev: (4137.50, 3921.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 31573)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (325.76, 147.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (318, 318, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (367.85, 126.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680550 reads in 165.775 CPU sec, 5.373 real sec
[M::mem_process_seqs] Processed 680652 reads in 116.314 CPU sec, 3.740 real sec
[M::process] read 686266 sequences (100000104 bp) in 2.775 CPU sec, 3.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286564, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 312, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (325.78, 147.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1083465 mem = 536878460 peak = 1072640000
[M::process] read 686012 sequences (100000199 bp) in 2.517 CPU sec, 2.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 287929, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 354, 4059)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11783)
[M::mem_pestat] mean and std.dev: (1490.93, 1759.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15645)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 282, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (299.41, 140.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 680934 reads in 172.335 CPU sec, 5.585 real sec
[M::mem_process_seqs] Processed 686266 reads in 118.272 CPU sec, 3.783 real sec
[M::process] read 683568 sequences (100000114 bp) in 2.469 CPU sec, 2.534 real sec...
sorting block 54, #reads = 1081110 mem = 536880607 peak = 1072640000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288067, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 283, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (300.24, 140.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287334, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 296, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (312.52, 144.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (292, 393, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (311.11, 115.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] skip orientation RR
[M::process] read 683122 sequences (100000121 bp) in 2.785 CPU sec, 3.428 real sec...
[M::mem_process_seqs] Processed 686012 reads in 162.649 CPU sec, 5.328 real sec
[M::mem_process_seqs] Processed 683568 reads in 111.947 CPU sec, 3.633 real sec
sorting block 55, #reads = 1088422 mem = 536879946 peak = 1072640000
[M::process] read 682734 sequences (100000135 bp) in 2.440 CPU sec, 2.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287310, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 299, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (314.66, 145.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682838 sequences (100000221 bp) in 2.539 CPU sec, 2.823 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286861, 7, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (317.29, 146.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (482, 482, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (482, 482)
[M::mem_pestat] mean and std.dev: (482.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (482, 482)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683122 reads in 161.384 CPU sec, 5.209 real sec
[M::mem_process_seqs] Processed 682734 reads in 111.451 CPU sec, 3.579 real sec
sorting block 56, #reads = 1087449 mem = 536879195 peak = 1072640000
[M::process] read 682596 sequences (100000161 bp) in 3.010 CPU sec, 3.308 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287185, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (318.20, 146.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (313, 313, 1168)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2878)
[M::mem_pestat] mean and std.dev: (613.45, 426.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3733)
[M::mem_pestat] skip orientation RR
sorting block 57, #reads = 1088267 mem = 536880864 peak = 1072640000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286838, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (319.22, 147.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (853, 853, 2499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5791)
[M::mem_pestat] mean and std.dev: (1300.82, 1008.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7437)
[M::mem_pestat] skip orientation RR
[M::process] read 682004 sequences (100000009 bp) in 2.280 CPU sec, 2.590 real sec...
[M::mem_process_seqs] Processed 682838 reads in 159.922 CPU sec, 5.235 real sec
[M::mem_process_seqs] Processed 682596 reads in 111.452 CPU sec, 3.675 real sec
[M::process] read 681826 sequences (100000045 bp) in 2.784 CPU sec, 3.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287199, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (322.85, 148.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1087159 mem = 536877137 peak = 1072640000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287048, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 308, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (322.99, 148.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681730 sequences (100000146 bp) in 2.653 CPU sec, 2.952 real sec...
[M::mem_process_seqs] Processed 682004 reads in 159.504 CPU sec, 5.216 real sec
[M::mem_process_seqs] Processed 681826 reads in 111.169 CPU sec, 3.667 real sec
[M::process] read 681484 sequences (100000223 bp) in 2.520 CPU sec, 2.760 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287116, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 310, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (323.66, 148.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 59, #reads = 1086330 mem = 536877795 peak = 1072640000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287052, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 310, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (324.49, 148.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681600 sequences (100000118 bp) in 2.387 CPU sec, 2.811 real sec...
[M::mem_process_seqs] Processed 681730 reads in 160.370 CPU sec, 5.349 real sec
[M::mem_process_seqs] Processed 681484 reads in 111.749 CPU sec, 3.727 real sec
sorting block 60, #reads = 1086184 mem = 536878912 peak = 1072640000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286802, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 310, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (323.97, 147.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681874 sequences (100000258 bp) in 2.838 CPU sec, 3.094 real sec...
[M::mem_process_seqs] Processed 681600 reads in 91.020 CPU sec, 3.056 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286849, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 306, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (320.78, 146.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681834 sequences (100000025 bp) in 2.872 CPU sec, 3.347 real sec...
[M::mem_process_seqs] Processed 681874 reads in 93.300 CPU sec, 3.195 real sec
sorting block 61, #reads = 1086140 mem = 536880626 peak = 1072640000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287111, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 306, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (321.52, 147.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680788 sequences (100000155 bp) in 2.601 CPU sec, 3.043 real sec...
[M::mem_process_seqs] Processed 681834 reads in 94.176 CPU sec, 3.170 real sec
sorting block 62, #reads = 1084697 mem = 536882196 peak = 1072640000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287062, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 313, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (326.61, 147.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680806 sequences (100000196 bp) in 2.848 CPU sec, 3.225 real sec...
[M::mem_process_seqs] Processed 680788 reads in 92.088 CPU sec, 3.138 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286851, 8, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (291, 1601, 1826)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4896)
[M::mem_pestat] mean and std.dev: (1574.80, 1395.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7157)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 311, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (324.55, 146.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 680578 sequences (100000199 bp) in 2.717 CPU sec, 3.032 real sec...
[M::mem_process_seqs] Processed 680806 reads in 90.942 CPU sec, 3.109 real sec
sorting block 63, #reads = 1085077 mem = 536877037 peak = 1072640000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286742, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 310, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (323.42, 144.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680552 sequences (100000159 bp) in 2.960 CPU sec, 3.170 real sec...
[M::mem_process_seqs] Processed 680578 reads in 93.501 CPU sec, 3.220 real sec
sorting block 64, #reads = 1086182 mem = 536877046 peak = 1072640000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286718, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 309, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (321.57, 143.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (366, 504, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 909)
[M::mem_pestat] mean and std.dev: (458.77, 172.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RR
[M::process] read 680696 sequences (100000168 bp) in 2.502 CPU sec, 2.877 real sec...
[M::mem_process_seqs] Processed 680552 reads in 91.563 CPU sec, 3.149 real sec
[M::process] read 681086 sequences (100000097 bp) in 2.544 CPU sec, 2.931 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286805, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (260, 260, 737)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1691)
[M::mem_pestat] mean and std.dev: (385.20, 254.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2168)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 306, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (319.19, 142.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 65, #reads = 1084562 mem = 536880254 peak = 1072640000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286660, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 305, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (318.29, 141.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680844 sequences (100000246 bp) in 2.345 CPU sec, 2.701 real sec...
[M::mem_process_seqs] Processed 680696 reads in 164.063 CPU sec, 5.375 real sec
[M::mem_process_seqs] Processed 681086 reads in 113.656 CPU sec, 3.701 real sec
[M::process] read 681022 sequences (100000299 bp) in 2.522 CPU sec, 2.649 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286407, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 308, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (321.09, 144.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 66, #reads = 1084879 mem = 536880509 peak = 1072640000
[M::process] read 680878 sequences (100000025 bp) in 2.410 CPU sec, 2.953 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286766, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 306, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (319.01, 143.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680844 reads in 165.425 CPU sec, 5.461 real sec
[M::mem_process_seqs] Processed 681022 reads in 114.302 CPU sec, 3.745 real sec
sorting block 67, #reads = 1083645 mem = 536879642 peak = 1072640000
[M::process] read 680484 sequences (100000088 bp) in 2.627 CPU sec, 2.769 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286094, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 307, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (320.23, 143.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680664 sequences (100000181 bp) in 2.408 CPU sec, 2.831 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286327, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 308, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (321.02, 143.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680878 reads in 165.952 CPU sec, 5.416 real sec
[M::mem_process_seqs] Processed 680484 reads in 114.947 CPU sec, 3.752 real sec
sorting block 68, #reads = 1083842 mem = 536879284 peak = 1072640000
[M::process] read 680382 sequences (100000076 bp) in 2.607 CPU sec, 2.742 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286686, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 308, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (321.87, 144.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 69, #reads = 1083175 mem = 536881338 peak = 1072640000
[M::process] read 680296 sequences (100000017 bp) in 2.494 CPU sec, 2.795 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287243, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 312, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (325.08, 145.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680664 reads in 164.344 CPU sec, 5.397 real sec
[M::mem_process_seqs] Processed 680382 reads in 113.087 CPU sec, 3.722 real sec
[M::process] read 679992 sequences (100000145 bp) in 2.336 CPU sec, 2.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286740, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 312, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836)
[M::mem_pestat] mean and std.dev: (325.09, 145.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 70, #reads = 1084172 mem = 536877795 peak = 1072640000
[M::process] read 680186 sequences (100000170 bp) in 2.615 CPU sec, 2.831 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286403, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 314, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (327.18, 147.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680296 reads in 166.649 CPU sec, 5.450 real sec
[M::mem_process_seqs] Processed 679992 reads in 117.456 CPU sec, 3.817 real sec
[M::process] read 687448 sequences (100000155 bp) in 2.482 CPU sec, 2.604 real sec...
sorting block 71, #reads = 1083271 mem = 536887592 peak = 1072640000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286579, 0, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 913, 1066)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2802)
[M::mem_pestat] mean and std.dev: (659.10, 479.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3670)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 314, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (327.07, 146.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 313, 1034)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2752)
[M::mem_pestat] mean and std.dev: (556.38, 405.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3611)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687722 sequences (100000126 bp) in 2.609 CPU sec, 3.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288691, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 275, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (295.59, 140.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680186 reads in 177.852 CPU sec, 5.761 real sec
[M::mem_process_seqs] Processed 687448 reads in 128.920 CPU sec, 4.166 real sec
[M::process] read 686946 sequences (100000160 bp) in 2.440 CPU sec, 2.536 real sec...
sorting block 72, #reads = 1083641 mem = 536880105 peak = 1072640000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288662, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 274, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (295.54, 141.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687128 sequences (100000223 bp) in 2.527 CPU sec, 2.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287853, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 280, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (300.50, 143.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687722 reads in 183.913 CPU sec, 5.931 real sec
[M::process] read 686114 sequences (100000040 bp) in 2.027 CPU sec, 2.087 real sec...
sorting block 73, #reads = 1082499 mem = 536880825 peak = 1072640000
[M::mem_process_seqs] Processed 686946 reads in 124.002 CPU sec, 3.990 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288860, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 280, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (298.93, 141.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 74, #reads = 1084166 mem = 536879592 peak = 1099661312
[M::process] read 685374 sequences (100000039 bp) in 2.825 CPU sec, 3.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288514, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 284, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (302.16, 141.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687128 reads in 178.836 CPU sec, 5.760 real sec
[M::mem_process_seqs] Processed 686114 reads in 120.998 CPU sec, 3.831 real sec
[M::process] read 684910 sequences (100000222 bp) in 2.369 CPU sec, 2.404 real sec...
sorting block 75, #reads = 1086019 mem = 536884971 peak = 1099661312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287871, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (307.23, 143.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684642 sequences (100000232 bp) in 2.653 CPU sec, 3.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287916, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (309.24, 144.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685374 reads in 172.743 CPU sec, 5.583 real sec
[M::process] read 684266 sequences (100000120 bp) in 2.284 CPU sec, 2.296 real sec...
[M::mem_process_seqs] Processed 684910 reads in 119.964 CPU sec, 3.825 real sec
sorting block 76, #reads = 1085013 mem = 536879599 peak = 1099661312
[M::process] read 683980 sequences (100000039 bp) in 2.824 CPU sec, 3.373 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287946, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 296, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (312.71, 145.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684266 reads in 99.195 CPU sec, 3.183 real sec
[M::process] read 683754 sequences (100000198 bp) in 2.419 CPU sec, 2.540 real sec...
sorting block 77, #reads = 1085156 mem = 536876820 peak = 1099661312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 288181, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (310.60, 144.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288130, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 296, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (313.04, 145.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684642 reads in 269.444 CPU sec, 8.710 real sec
[M::mem_process_seqs] Processed 683980 reads in 139.941 CPU sec, 4.513 real sec
[M::process] read 684304 sequences (100000283 bp) in 2.663 CPU sec, 2.741 real sec...
sorting block 78, #reads = 1086009 mem = 536878532 peak = 1099661312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287821, 9, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (366, 366, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (366, 366)
[M::mem_pestat] mean and std.dev: (366.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (366, 366)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 299, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (314.52, 145.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 684220 sequences (100000290 bp) in 2.867 CPU sec, 3.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287667, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 297, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (313.01, 145.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683754 reads in 174.044 CPU sec, 5.663 real sec
[M::mem_process_seqs] Processed 684304 reads in 120.309 CPU sec, 3.898 real sec
sorting block 79, #reads = 1085566 mem = 536877501 peak = 1099661312
[M::process] read 683750 sequences (100000168 bp) in 2.610 CPU sec, 2.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287395, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 297, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (313.58, 146.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683538 sequences (100000126 bp) in 2.590 CPU sec, 2.759 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 288389, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 300, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (315.33, 145.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684220 reads in 178.573 CPU sec, 5.712 real sec
[M::process] read 683562 sequences (100000004 bp) in 2.364 CPU sec, 2.418 real sec...
[M::mem_process_seqs] Processed 683750 reads in 122.080 CPU sec, 3.874 real sec
sorting block 80, #reads = 1084121 mem = 536879040 peak = 1099661312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287659, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 299, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (315.03, 145.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 81, #reads = 1084789 mem = 536879390 peak = 1124016128
[M::process] read 683724 sequences (100000116 bp) in 3.001 CPU sec, 3.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287643, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 300, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (315.58, 146.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683538 reads in 178.521 CPU sec, 5.775 real sec
[M::mem_process_seqs] Processed 683562 reads in 122.159 CPU sec, 3.906 real sec
[M::process] read 683050 sequences (100000203 bp) in 2.435 CPU sec, 2.496 real sec...
sorting block 82, #reads = 1083574 mem = 536883247 peak = 1124016128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287884, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 300, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (316.29, 147.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1062)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 314, 908)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2292)
[M::mem_pestat] mean and std.dev: (527.00, 371.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2984)
[M::mem_pestat] skip orientation RR
[M::process] read 682976 sequences (100000058 bp) in 2.618 CPU sec, 2.969 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287456, 5, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (264, 339, 895)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2157)
[M::mem_pestat] mean and std.dev: (541.18, 325.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2788)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 301, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (316.02, 145.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 683724 reads in 179.666 CPU sec, 5.872 real sec
[M::process] read 682274 sequences (100000165 bp) in 2.358 CPU sec, 2.519 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286999, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 304, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (318.39, 146.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682976 reads in 103.453 CPU sec, 3.305 real sec
sorting block 83, #reads = 1083327 mem = 536878891 peak = 1124016128
[M::process] read 681870 sequences (100000030 bp) in 2.764 CPU sec, 2.905 real sec...
[M::mem_process_seqs] Processed 683050 reads in 282.309 CPU sec, 9.125 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287366, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (318.16, 144.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 84, #reads = 1083023 mem = 536878298 peak = 1136488448
[M::process] read 681996 sequences (100000253 bp) in 2.493 CPU sec, 2.868 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286891, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (317.95, 143.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682274 reads in 205.386 CPU sec, 6.595 real sec
[M::mem_process_seqs] Processed 681870 reads in 124.301 CPU sec, 3.987 real sec
[M::process] read 681506 sequences (100000235 bp) in 2.501 CPU sec, 2.601 real sec...
sorting block 85, #reads = 1081808 mem = 536879230 peak = 1136488448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 287529, 10, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (250, 440, 852)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2056)
[M::mem_pestat] mean and std.dev: (375.73, 239.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2658)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 305, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (318.46, 143.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (305, 835, 1238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3104)
[M::mem_pestat] mean and std.dev: (705.80, 458.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4037)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 681160 sequences (100000145 bp) in 2.871 CPU sec, 3.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287199, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 307, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (319.89, 143.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681996 reads in 180.382 CPU sec, 5.831 real sec
[M::mem_process_seqs] Processed 681506 reads in 125.392 CPU sec, 4.016 real sec
[M::process] read 681578 sequences (100000008 bp) in 2.652 CPU sec, 2.746 real sec...
sorting block 86, #reads = 1082750 mem = 536878629 peak = 1136488448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286601, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 310, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (322.49, 144.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681566 sequences (100000066 bp) in 2.828 CPU sec, 3.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286764, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 306, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (319.62, 143.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 278, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (212, 377)
[M::mem_pestat] mean and std.dev: (283.50, 12.30)
[M::mem_pestat] low and high boundaries for proper pairs: (179, 410)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681160 reads in 182.456 CPU sec, 5.922 real sec
[M::mem_process_seqs] Processed 681578 reads in 126.485 CPU sec, 4.067 real sec
sorting block 87, #reads = 1081647 mem = 536882136 peak = 1136488448
[M::process] read 681552 sequences (100000156 bp) in 2.453 CPU sec, 2.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287263, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 308, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (321.87, 145.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689876 sequences (100000244 bp) in 2.874 CPU sec, 3.396 real sec...
sorting block 88, #reads = 1081048 mem = 536876699 peak = 1136488448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286569, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 307, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (321.40, 146.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681566 reads in 182.848 CPU sec, 5.915 real sec
[M::mem_process_seqs] Processed 681552 reads in 127.521 CPU sec, 4.082 real sec
[M::process] read 691858 sequences (100000289 bp) in 2.628 CPU sec, 2.756 real sec...
sorting block 89, #reads = 1082141 mem = 536876949 peak = 1136488448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 289209, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 266, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (285.90, 135.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691218 sequences (100000170 bp) in 2.854 CPU sec, 3.013 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289990, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 257, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (276.72, 130.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690240 sequences (100000063 bp) in 2.290 CPU sec, 2.299 real sec...
[M::mem_process_seqs] Processed 689876 reads in 221.019 CPU sec, 7.086 real sec
[M::mem_process_seqs] Processed 691858 reads in 155.626 CPU sec, 4.954 real sec
sorting block 90, #reads = 1078667 mem = 536877855 peak = 1136488448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290265, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 261, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (281.65, 133.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689050 sequences (100000010 bp) in 2.862 CPU sec, 3.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289719, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 265, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (285.77, 135.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687438 sequences (100000187 bp) in 2.559 CPU sec, 2.569 real sec...
[M::mem_process_seqs] Processed 691218 reads in 212.375 CPU sec, 6.801 real sec
[M::mem_process_seqs] Processed 690240 reads in 143.369 CPU sec, 4.575 real sec
sorting block 91, #reads = 1076647 mem = 536880261 peak = 1136488448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 289075, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 271, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (289.51, 136.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687440 sequences (100000262 bp) in 2.635 CPU sec, 3.078 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288808, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 278, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (294.96, 136.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686388 sequences (100000281 bp) in 2.364 CPU sec, 2.372 real sec...
[M::mem_process_seqs] Processed 689050 reads in 207.503 CPU sec, 6.700 real sec
sorting block 92, #reads = 1078986 mem = 536878998 peak = 1136488448
[M::mem_process_seqs] Processed 687438 reads in 140.697 CPU sec, 4.500 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289218, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 279, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (296.12, 137.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 93, #reads = 1079698 mem = 536879395 peak = 1140088832
[M::process] read 686348 sequences (100000129 bp) in 3.193 CPU sec, 3.764 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288332, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 284, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (299.92, 138.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687440 reads in 199.620 CPU sec, 6.415 real sec
[M::process] read 685484 sequences (100000236 bp) in 2.407 CPU sec, 2.435 real sec...
[M::mem_process_seqs] Processed 686388 reads in 135.485 CPU sec, 4.319 real sec
sorting block 94, #reads = 1080246 mem = 536880313 peak = 1140088832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 288792, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 286, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (301.56, 139.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685258 sequences (100000191 bp) in 2.861 CPU sec, 3.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288437, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 288, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (303.98, 140.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686348 reads in 194.970 CPU sec, 6.358 real sec
[M::process] read 684826 sequences (100000278 bp) in 2.351 CPU sec, 2.403 real sec...
[M::mem_process_seqs] Processed 685484 reads in 132.068 CPU sec, 4.341 real sec
sorting block 95, #reads = 1080322 mem = 536877914 peak = 1140088832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 288527, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (306.11, 141.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684688 sequences (100000167 bp) in 2.770 CPU sec, 3.115 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287683, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 291, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (306.31, 140.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685258 reads in 189.296 CPU sec, 6.118 real sec
[M::process] read 684536 sequences (100000224 bp) in 2.467 CPU sec, 2.685 real sec...
[M::mem_process_seqs] Processed 684826 reads in 129.154 CPU sec, 4.134 real sec
sorting block 96, #reads = 1081955 mem = 536878542 peak = 1140088832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 288092, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (306.52, 141.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683924 sequences (100000267 bp) in 2.418 CPU sec, 2.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287941, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 292, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (308.31, 142.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683550 sequences (100000126 bp) in 2.301 CPU sec, 2.316 real sec...
[M::mem_process_seqs] Processed 684688 reads in 186.784 CPU sec, 5.977 real sec
sorting block 97, #reads = 1081679 mem = 536881485 peak = 1140088832
[M::mem_process_seqs] Processed 684536 reads in 126.820 CPU sec, 4.031 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287663, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 296, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (312.21, 145.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 98, #reads = 1081895 mem = 536878409 peak = 1184387072
[M::process] read 683110 sequences (100000189 bp) in 3.047 CPU sec, 3.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287689, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 299, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (316.63, 147.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683924 reads in 184.888 CPU sec, 5.960 real sec
[M::process] read 682902 sequences (100000256 bp) in 2.592 CPU sec, 2.634 real sec...
[M::mem_process_seqs] Processed 683550 reads in 127.258 CPU sec, 4.052 real sec
sorting block 99, #reads = 1081716 mem = 536878467 peak = 1184387072
[M::process] read 682306 sequences (100000051 bp) in 2.656 CPU sec, 3.328 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287399, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 301, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (317.85, 147.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682902 reads in 103.576 CPU sec, 3.335 real sec
[M::process] read 682298 sequences (100000003 bp) in 2.411 CPU sec, 2.524 real sec...
sorting block 100, #reads = 1083653 mem = 536879744 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287859, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 301, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (318.89, 149.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287148, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (319.75, 147.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1059)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683110 reads in 280.736 CPU sec, 9.120 real sec
[M::mem_process_seqs] Processed 682306 reads in 145.031 CPU sec, 4.671 real sec
[M::process] read 681978 sequences (100000098 bp) in 2.775 CPU sec, 2.869 real sec...
sorting block 101, #reads = 1082470 mem = 536877903 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287001, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (319.45, 147.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681988 sequences (100000095 bp) in 2.832 CPU sec, 3.394 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286382, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 306, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (322.43, 149.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682298 reads in 180.483 CPU sec, 5.845 real sec
[M::mem_process_seqs] Processed 681978 reads in 124.298 CPU sec, 4.014 real sec
[M::process] read 681688 sequences (100000045 bp) in 2.823 CPU sec, 2.931 real sec...
sorting block 102, #reads = 1082781 mem = 536876596 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286737, 10, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (323.36, 150.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (745, 745, 745)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (745, 745)
[M::mem_pestat] mean and std.dev: (745.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (745, 745)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 681246 sequences (100000188 bp) in 2.811 CPU sec, 3.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286737, 1, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 467, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1179)
[M::mem_pestat] mean and std.dev: (375.00, 263.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1511)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 306, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (323.80, 150.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 681988 reads in 179.583 CPU sec, 5.775 real sec
[M::mem_process_seqs] Processed 681688 reads in 123.037 CPU sec, 3.909 real sec
[M::process] read 684950 sequences (100000035 bp) in 2.512 CPU sec, 2.543 real sec...
sorting block 103, #reads = 1081909 mem = 536879843 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286751, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 311, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (327.13, 151.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688056 sequences (100000135 bp) in 3.359 CPU sec, 3.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287480, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 288, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (307.46, 145.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681246 reads in 202.921 CPU sec, 6.448 real sec
sorting block 104, #reads = 1081406 mem = 536877632 peak = 1184387072
[M::process] read 686978 sequences (100000025 bp) in 2.319 CPU sec, 2.342 real sec...
[M::mem_process_seqs] Processed 684950 reads in 150.146 CPU sec, 4.757 real sec
sorting block 105, #reads = 1073771 mem = 536880906 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 289073, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 273, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (293.48, 139.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685774 sequences (100000134 bp) in 2.867 CPU sec, 3.130 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288125, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 281, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (299.28, 141.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688056 reads in 173.476 CPU sec, 5.528 real sec
[M::process] read 685498 sequences (100000207 bp) in 2.290 CPU sec, 2.292 real sec...
[M::mem_process_seqs] Processed 686978 reads in 113.731 CPU sec, 3.572 real sec
sorting block 106, #reads = 1082993 mem = 536885053 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288752, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 287, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (303.23, 141.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685116 sequences (100000287 bp) in 2.550 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287959, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 287, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (304.32, 141.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685774 reads in 166.567 CPU sec, 5.276 real sec
[M::process] read 684434 sequences (100000292 bp) in 2.238 CPU sec, 2.239 real sec...
[M::mem_process_seqs] Processed 685498 reads in 114.343 CPU sec, 3.619 real sec
sorting block 107, #reads = 1084978 mem = 536877955 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288132, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 290, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (306.78, 143.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684358 sequences (100000155 bp) in 2.789 CPU sec, 2.919 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287563, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 292, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (308.09, 142.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685116 reads in 168.971 CPU sec, 5.378 real sec
[M::process] read 684052 sequences (100000227 bp) in 2.396 CPU sec, 2.419 real sec...
[M::mem_process_seqs] Processed 684434 reads in 116.223 CPU sec, 3.660 real sec
sorting block 108, #reads = 1085606 mem = 536882192 peak = 1184387072
sorting block 109, #reads = 1085894 mem = 536880056 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287962, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 293, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (308.11, 141.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683298 sequences (100000070 bp) in 2.410 CPU sec, 2.470 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288050, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 296, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (311.45, 143.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684358 reads in 153.781 CPU sec, 4.875 real sec
[M::mem_process_seqs] Processed 684052 reads in 101.427 CPU sec, 3.178 real sec
[M::process] read 683432 sequences (100000254 bp) in 2.300 CPU sec, 2.307 real sec...
sorting block 110, #reads = 1085104 mem = 536879803 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287576, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 298, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (312.64, 143.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683492 sequences (100000110 bp) in 2.734 CPU sec, 2.793 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 287440, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 298, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (313.49, 144.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683298 reads in 152.427 CPU sec, 4.823 real sec
[M::mem_process_seqs] Processed 683432 reads in 105.688 CPU sec, 3.321 real sec
[M::process] read 684454 sequences (100000160 bp) in 2.385 CPU sec, 2.415 real sec...
sorting block 111, #reads = 1085575 mem = 536878119 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287630, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 299, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (314.06, 144.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683742 sequences (100000246 bp) in 2.596 CPU sec, 2.744 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287703, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (308.05, 143.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683492 reads in 165.514 CPU sec, 5.293 real sec
[M::mem_process_seqs] Processed 684454 reads in 115.508 CPU sec, 3.659 real sec
[M::process] read 683884 sequences (100000148 bp) in 2.428 CPU sec, 2.494 real sec...
sorting block 112, #reads = 1085291 mem = 536876280 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287667, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 296, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (312.34, 145.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683168 sequences (100000060 bp) in 3.049 CPU sec, 3.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287905, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 296, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (312.90, 145.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683742 reads in 170.609 CPU sec, 5.474 real sec
[M::mem_process_seqs] Processed 683884 reads in 118.984 CPU sec, 3.768 real sec
sorting block 113, #reads = 1085025 mem = 536876803 peak = 1184387072
[M::process] read 682456 sequences (100000176 bp) in 3.005 CPU sec, 3.128 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 287091, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 676, 862)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2218)
[M::mem_pestat] mean and std.dev: (558.23, 532.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2896)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 299, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (315.19, 145.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 114, #reads = 1084209 mem = 536879864 peak = 1184387072
[M::process] read 682266 sequences (100000187 bp) in 2.417 CPU sec, 2.561 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287079, 9, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (319.22, 147.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683168 reads in 170.783 CPU sec, 5.451 real sec
[M::mem_process_seqs] Processed 682456 reads in 115.607 CPU sec, 3.643 real sec
[M::process] read 682132 sequences (100000066 bp) in 2.965 CPU sec, 3.078 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286874, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 305, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (321.19, 148.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 115, #reads = 1083124 mem = 536880054 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286710, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (320.63, 148.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682148 sequences (100000166 bp) in 3.227 CPU sec, 3.432 real sec...
[M::mem_process_seqs] Processed 682266 reads in 161.142 CPU sec, 5.151 real sec
[M::mem_process_seqs] Processed 682132 reads in 106.981 CPU sec, 3.377 real sec
sorting block 116, #reads = 1083697 mem = 536877512 peak = 1184387072
[M::process] read 682304 sequences (100000088 bp) in 2.452 CPU sec, 2.537 real sec...
[M::process] read 682354 sequences (100000190 bp) in 1.849 CPU sec, 1.854 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286963, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (319.46, 148.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (322, 582, 1360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3436)
[M::mem_pestat] mean and std.dev: (651.83, 630.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4474)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682304 reads in 82.743 CPU sec, 2.587 real sec
[M::process] read 681722 sequences (100000278 bp) in 1.961 CPU sec, 1.988 real sec...
sorting block 117, #reads = 1082451 mem = 536880296 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287153, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (320.32, 148.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287065, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 300, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (317.32, 146.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682148 reads in 230.652 CPU sec, 7.274 real sec
[M::mem_process_seqs] Processed 682354 reads in 117.709 CPU sec, 3.711 real sec
[M::process] read 681772 sequences (100000129 bp) in 2.339 CPU sec, 2.371 real sec...
sorting block 118, #reads = 1081432 mem = 536878349 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287008, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (320.32, 147.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681910 sequences (100000057 bp) in 2.403 CPU sec, 2.451 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286973, 10, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 305, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (320.86, 147.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 1052, 1052)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3142)
[M::mem_pestat] mean and std.dev: (539.70, 512.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4187)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 681722 reads in 150.680 CPU sec, 4.769 real sec
[M::process] read 682012 sequences (100000286 bp) in 2.042 CPU sec, 2.060 real sec...
[M::mem_process_seqs] Processed 681772 reads in 103.654 CPU sec, 3.254 real sec
sorting block 119, #reads = 1081960 mem = 536879455 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 286778, 0, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 376, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (304.31, 96.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (320.79, 148.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 681606 sequences (100000157 bp) in 2.463 CPU sec, 2.564 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286339, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 303, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (319.78, 147.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (504, 1169, 2204)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5604)
[M::mem_pestat] mean and std.dev: (1086.09, 635.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7304)
[M::mem_pestat] skip orientation RR
[M::process] read 684908 sequences (100000099 bp) in 1.750 CPU sec, 1.745 real sec...
[M::mem_process_seqs] Processed 681910 reads in 150.247 CPU sec, 4.742 real sec
sorting block 120, #reads = 1081717 mem = 536877773 peak = 1184387072
[M::mem_process_seqs] Processed 682012 reads in 96.448 CPU sec, 3.045 real sec
sorting block 121, #reads = 1079295 mem = 536879875 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286957, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 305, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (321.68, 148.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1057)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 693882 sequences (100000001 bp) in 2.144 CPU sec, 2.179 real sec...
[M::process] read 690084 sequences (100000250 bp) in 1.720 CPU sec, 1.725 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288003, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 289, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (308.17, 145.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681606 reads in 156.392 CPU sec, 4.952 real sec
[M::mem_process_seqs] Processed 684908 reads in 113.909 CPU sec, 3.610 real sec
sorting block 122, #reads = 1075683 mem = 536879462 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290155, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 249, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726)
[M::mem_pestat] mean and std.dev: (271.19, 130.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688636 sequences (100000261 bp) in 2.610 CPU sec, 2.657 real sec...
[M::process] read 687458 sequences (100000238 bp) in 2.270 CPU sec, 2.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290114, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 268, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (288.95, 137.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 693882 reads in 186.616 CPU sec, 5.890 real sec
[M::mem_process_seqs] Processed 690084 reads in 124.056 CPU sec, 3.934 real sec
sorting block 123, #reads = 1074701 mem = 536880886 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 289679, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 275, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (293.05, 137.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686404 sequences (100000230 bp) in 2.776 CPU sec, 2.820 real sec...
[M::process] read 685482 sequences (100000060 bp) in 2.420 CPU sec, 2.417 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 289551, 1, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (452, 483, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (390, 545)
[M::mem_pestat] mean and std.dev: (477.83, 11.55)
[M::mem_pestat] low and high boundaries for proper pairs: (359, 576)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 279, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (297.69, 140.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 688636 reads in 191.519 CPU sec, 6.042 real sec
[M::mem_process_seqs] Processed 687458 reads in 132.097 CPU sec, 4.192 real sec
sorting block 124, #reads = 1078764 mem = 536879940 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288796, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 285, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (302.03, 141.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685008 sequences (100000028 bp) in 3.123 CPU sec, 3.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 288310, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 208, 240)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418)
[M::mem_pestat] mean and std.dev: (198.89, 43.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 507)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 290, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (305.74, 141.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (309, 309, 981)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2325)
[M::mem_pestat] mean and std.dev: (501.10, 386.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2997)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684428 sequences (100000083 bp) in 2.385 CPU sec, 2.390 real sec...
[M::mem_process_seqs] Processed 686404 reads in 192.438 CPU sec, 6.096 real sec
sorting block 125, #reads = 1079852 mem = 536882838 peak = 1184387072
[M::mem_process_seqs] Processed 685482 reads in 132.788 CPU sec, 4.194 real sec
sorting block 126, #reads = 1078357 mem = 536878353 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 288249, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (309.21, 143.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683684 sequences (100000291 bp) in 3.033 CPU sec, 3.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287823, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 297, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (312.14, 144.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684186 sequences (100000148 bp) in 2.291 CPU sec, 2.306 real sec...
[M::mem_process_seqs] Processed 685008 reads in 188.228 CPU sec, 5.983 real sec
[M::mem_process_seqs] Processed 684428 reads in 129.349 CPU sec, 4.109 real sec
sorting block 127, #reads = 1078858 mem = 536878976 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287883, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 300, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (314.62, 144.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682708 sequences (100000298 bp) in 2.841 CPU sec, 3.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 288304, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 348, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (275.50, 101.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 295, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (311.93, 144.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 683684 reads in 192.034 CPU sec, 6.374 real sec
[M::process] read 682188 sequences (100000057 bp) in 2.215 CPU sec, 2.314 real sec...
[M::mem_process_seqs] Processed 684186 reads in 130.735 CPU sec, 4.457 real sec
sorting block 128, #reads = 1079134 mem = 536878413 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287731, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 302, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (317.23, 145.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682282 sequences (100000170 bp) in 3.187 CPU sec, 3.936 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287569, 0, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 307, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (321.42, 147.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (325, 424, 803)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1759)
[M::mem_pestat] mean and std.dev: (457.83, 267.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2237)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682708 reads in 188.624 CPU sec, 6.389 real sec
[M::mem_process_seqs] Processed 682188 reads in 129.624 CPU sec, 4.423 real sec
sorting block 129, #reads = 1080972 mem = 536880611 peak = 1184387072
[M::process] read 682126 sequences (100000072 bp) in 2.535 CPU sec, 2.764 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287567, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 306, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (320.19, 146.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1063)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681808 sequences (100000201 bp) in 2.793 CPU sec, 3.471 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286630, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 304, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (319.07, 146.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682282 reads in 189.503 CPU sec, 6.439 real sec
sorting block 130, #reads = 1080950 mem = 536883973 peak = 1184387072
[M::mem_process_seqs] Processed 682126 reads in 132.007 CPU sec, 4.447 real sec
[M::process] read 681698 sequences (100000106 bp) in 2.413 CPU sec, 2.690 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286902, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 302, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (317.63, 144.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 131, #reads = 1079973 mem = 536876925 peak = 1184387072
[M::process] read 681954 sequences (100000183 bp) in 2.843 CPU sec, 3.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286792, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (319.42, 145.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681808 reads in 184.239 CPU sec, 6.304 real sec
[M::mem_process_seqs] Processed 681698 reads in 127.525 CPU sec, 4.421 real sec
[M::process] read 681572 sequences (100000033 bp) in 2.543 CPU sec, 2.859 real sec...
sorting block 132, #reads = 1080987 mem = 536877014 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286544, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 303, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (319.26, 146.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681742 sequences (100000143 bp) in 2.710 CPU sec, 3.092 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287044, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 303, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (318.48, 144.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681954 reads in 180.551 CPU sec, 6.098 real sec
[M::mem_process_seqs] Processed 681572 reads in 124.973 CPU sec, 4.292 real sec
[M::process] read 681330 sequences (100000005 bp) in 2.347 CPU sec, 2.503 real sec...
sorting block 133, #reads = 1081045 mem = 536876880 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286748, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 304, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (319.42, 146.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1048)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681372 sequences (100000173 bp) in 2.789 CPU sec, 3.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286297, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 305, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838)
[M::mem_pestat] mean and std.dev: (320.41, 146.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681742 reads in 177.771 CPU sec, 6.023 real sec
[M::mem_process_seqs] Processed 681330 reads in 123.164 CPU sec, 4.193 real sec
sorting block 134, #reads = 1081605 mem = 536878644 peak = 1184387072
[M::process] read 681432 sequences (100000197 bp) in 2.537 CPU sec, 2.855 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286998, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 307, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (322.40, 147.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1054)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 135, #reads = 1082221 mem = 536880629 peak = 1184387072
[M::process] read 680976 sequences (100000064 bp) in 2.462 CPU sec, 2.772 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286957, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 306, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (321.91, 147.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681372 reads in 177.393 CPU sec, 6.138 real sec
[M::mem_process_seqs] Processed 681432 reads in 122.553 CPU sec, 4.342 real sec
[M::process] read 680968 sequences (100000279 bp) in 2.315 CPU sec, 2.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 286690, 13, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 1701, 1956)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5542)
[M::mem_pestat] mean and std.dev: (1520.23, 1155.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7335)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 308, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (323.84, 148.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1, 929, 4187)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12559)
[M::mem_pestat] mean and std.dev: (1637.69, 1814.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 16745)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
sorting block 136, #reads = 1082713 mem = 536879978 peak = 1184387072
[M::process] read 680976 sequences (100000098 bp) in 2.794 CPU sec, 3.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286909, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 310, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (325.08, 149.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (121, 121, 161)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (41, 241)
[M::mem_pestat] mean and std.dev: (128.50, 19.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 281)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680976 reads in 174.724 CPU sec, 5.945 real sec
[M::process] read 180042 sequences (26349911 bp) in 0.845 CPU sec, 0.879 real sec...
[M::mem_process_seqs] Processed 680968 reads in 119.190 CPU sec, 4.123 real sec
sorting block 137, #reads = 1082229 mem = 536880487 peak = 1184387072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286688, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 309, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (325.73, 150.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 75886, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 296, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (313.23, 145.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680976 reads in 119.466 CPU sec, 4.271 real sec
[M::mem_process_seqs] Processed 180042 reads in 51.795 CPU sec, 1.949 real sec
sorting block 138, #reads = 1083215 mem = 536879699 peak = 1184387072
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 150437348
overall: 69776990208 mem 23012.591 user 439.468 sys 781.906 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_cAd-T1-TDNA-1_A23MHWWLT4_1.L005\tPU:L005\tSM:659_cAd-T1-TDNA-1_A23MHWWLT4_1\tLB:659_cAd-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_cAd-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_cAd-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 781.946 sec; CPU: 23452.063 sec
sorting block 139, #reads = 402232 mem = 200052388 peak = 1184387072
uniq header count 1
merging 140 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8209303854
output reads: 151019834
bam_mem_sort: 140 calls 92.093 user 1.835 sys 97.689 real
bam_write: 140 calls 94.566 user 6.259 sys 205.842 real
execute: 1 calls 59.197 user 3.897 sys 886.364 real
merge_files: 1 calls 59.162 user 3.871 sys 104.071 real
parse_chunk: 2230 calls 136.800 user 30.397 sys 211.988 real
read_chunk: 6012 calls 80.720 user 33.684 sys 754.760 real
sort_block: 1 calls 53.150 user 32.819 sys 707.805 real
write_chunk: 3780 calls 58.524 user 3.439 sys 102.340 real
overall: 3214700544 mem 2883.088 user 102.437 sys 886.388 real
ls: cannot access '*.cram': No such file or directory