.command.err - Workdir - DAQ:SENTIEON_BWAMEM (659_bR4-T1-TDNA-1_A23MHWWLT4_1;L004;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/58/dded53c21127e193a45e1bbd42a63b/.command.err
Size: 241.9 KB
Task: DAQ:SENTIEON_BWAMEM (659_bR4-T1-TDNA-1_A23MHWWLT4_1;L004;0001)
Attempt
Content

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_bR4-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1214.979 CPU sec, 42.441 real sec
[M::process] read 678068 sequences (100000296 bp) in 1.244 CPU sec, 1.252 real sec...
[M::process] read 677742 sequences (100000209 bp) in 1.250 CPU sec, 1.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282805, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 327, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (344.20, 156.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677708 sequences (100000085 bp) in 1.310 CPU sec, 1.312 real sec...
[M::process] read 677510 sequences (100000200 bp) in 1.380 CPU sec, 1.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282178, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (347.88, 157.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678068 reads in 149.195 CPU sec, 4.690 real sec
[M::mem_process_seqs] Processed 677742 reads in 99.988 CPU sec, 3.173 real sec
sorting block 0, #reads = 1081973 mem = 536879959 peak = 606412800
[M::process] read 677642 sequences (100000132 bp) in 1.984 CPU sec, 2.011 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282057, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (347.98, 156.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677094 sequences (100000086 bp) in 1.590 CPU sec, 1.591 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282521, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (346.94, 155.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677708 reads in 147.534 CPU sec, 4.659 real sec
[M::mem_process_seqs] Processed 677510 reads in 165.053 CPU sec, 5.230 real sec
sorting block 1, #reads = 1080949 mem = 536880909 peak = 734846976
[M::process] read 676894 sequences (100000137 bp) in 1.903 CPU sec, 1.957 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282779, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 333, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (348.37, 156.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676648 sequences (100000291 bp) in 1.360 CPU sec, 1.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281904, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 335, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (350.89, 156.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282036, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 338, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (354.09, 158.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677642 reads in 209.979 CPU sec, 6.683 real sec
[M::mem_process_seqs] Processed 677094 reads in 161.287 CPU sec, 5.166 real sec
sorting block 2, #reads = 1079629 mem = 536878272 peak = 851697664
[M::mem_process_seqs] Processed 676894 reads in 179.322 CPU sec, 5.753 real sec
sorting block 3, #reads = 1079679 mem = 536878025 peak = 851697664
[M::process] read 676346 sequences (100000176 bp) in 2.606 CPU sec, 2.682 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281887, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 339, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (354.75, 158.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676104 sequences (100000220 bp) in 1.880 CPU sec, 1.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282387, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 342, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (357.67, 159.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676648 reads in 150.384 CPU sec, 4.758 real sec
[M::mem_process_seqs] Processed 676346 reads in 100.352 CPU sec, 3.153 real sec
sorting block 4, #reads = 1080243 mem = 536878425 peak = 851697664
[M::process] read 675742 sequences (100000236 bp) in 2.249 CPU sec, 2.332 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281595, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 344, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (359.61, 160.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675648 sequences (100000150 bp) in 2.089 CPU sec, 2.108 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281311, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (363.10, 162.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676104 reads in 146.152 CPU sec, 4.609 real sec
[M::process] read 675602 sequences (100000054 bp) in 1.965 CPU sec, 1.977 real sec...
[M::mem_process_seqs] Processed 675742 reads in 97.628 CPU sec, 3.059 real sec
sorting block 5, #reads = 1080752 mem = 536881025 peak = 851697664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281842, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 349, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (364.20, 162.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675480 sequences (100000293 bp) in 2.229 CPU sec, 2.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281451, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (365.49, 162.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675286 sequences (100000066 bp) in 1.890 CPU sec, 1.888 real sec...
[M::mem_process_seqs] Processed 675648 reads in 148.931 CPU sec, 4.704 real sec
sorting block 6, #reads = 1080044 mem = 536876957 peak = 851697664
[M::mem_process_seqs] Processed 675602 reads in 99.259 CPU sec, 3.130 real sec
sorting block 7, #reads = 1080678 mem = 536877552 peak = 910544896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281594, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 351, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (366.55, 163.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675382 sequences (100000256 bp) in 2.437 CPU sec, 2.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281364, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 352, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (367.18, 163.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675228 sequences (100000006 bp) in 1.890 CPU sec, 1.895 real sec...
[M::mem_process_seqs] Processed 675480 reads in 149.644 CPU sec, 4.718 real sec
[M::mem_process_seqs] Processed 675286 reads in 99.588 CPU sec, 3.142 real sec
sorting block 8, #reads = 1079638 mem = 536880015 peak = 910544896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281236, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (365.42, 162.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675272 sequences (100000132 bp) in 2.031 CPU sec, 2.094 real sec...
[M::process] read 675450 sequences (100000108 bp) in 1.440 CPU sec, 1.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280914, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (365.23, 162.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675382 reads in 151.191 CPU sec, 4.798 real sec
[M::mem_process_seqs] Processed 675228 reads in 100.841 CPU sec, 3.197 real sec
sorting block 9, #reads = 1080044 mem = 536879854 peak = 910544896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281629, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (364.16, 161.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675270 sequences (100000059 bp) in 2.436 CPU sec, 2.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280977, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (363.10, 160.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675392 sequences (100000024 bp) in 1.980 CPU sec, 1.974 real sec...
[M::mem_process_seqs] Processed 675272 reads in 149.010 CPU sec, 4.695 real sec
sorting block 10, #reads = 1079792 mem = 536878578 peak = 910544896
[M::mem_process_seqs] Processed 675450 reads in 99.254 CPU sec, 3.125 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281217, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (363.26, 160.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675334 sequences (100000122 bp) in 2.058 CPU sec, 2.107 real sec...
[M::process] read 675572 sequences (100000246 bp) in 1.640 CPU sec, 1.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280997, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 344, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (360.18, 158.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (338, 402, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (210, 530)
[M::mem_pestat] mean and std.dev: (380.10, 28.42)
[M::mem_pestat] low and high boundaries for proper pairs: (146, 594)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675270 reads in 150.544 CPU sec, 4.767 real sec
sorting block 11, #reads = 1078881 mem = 536879407 peak = 910544896
[M::mem_process_seqs] Processed 675392 reads in 99.750 CPU sec, 3.166 real sec
sorting block 12, #reads = 1079873 mem = 536880004 peak = 963514368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280855, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (362.00, 160.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675410 sequences (100000221 bp) in 2.595 CPU sec, 2.635 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281814, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 344, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (360.65, 160.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675358 sequences (100000165 bp) in 1.940 CPU sec, 1.941 real sec...
[M::mem_process_seqs] Processed 675334 reads in 153.699 CPU sec, 4.847 real sec
[M::mem_process_seqs] Processed 675572 reads in 101.246 CPU sec, 3.185 real sec
sorting block 13, #reads = 1079537 mem = 536880901 peak = 963514368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280757, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (361.13, 160.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675274 sequences (100000122 bp) in 2.354 CPU sec, 2.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281027, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (362.14, 161.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674972 sequences (100000242 bp) in 1.470 CPU sec, 1.470 real sec...
[M::mem_process_seqs] Processed 675410 reads in 146.943 CPU sec, 4.650 real sec
[M::mem_process_seqs] Processed 675358 reads in 95.592 CPU sec, 3.023 real sec
sorting block 14, #reads = 1079879 mem = 536879710 peak = 963514368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281261, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (362.73, 161.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674994 sequences (100000147 bp) in 2.557 CPU sec, 2.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280906, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (363.40, 161.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675274 reads in 146.360 CPU sec, 4.612 real sec
[M::process] read 674840 sequences (100000181 bp) in 1.999 CPU sec, 1.997 real sec...
sorting block 15, #reads = 1079004 mem = 536879126 peak = 963514368
[M::mem_process_seqs] Processed 674972 reads in 98.540 CPU sec, 3.092 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280852, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (365.62, 161.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674600 sequences (100000147 bp) in 2.315 CPU sec, 2.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281261, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (364.46, 160.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674994 reads in 148.602 CPU sec, 4.688 real sec
[M::process] read 674446 sequences (100000163 bp) in 1.950 CPU sec, 1.945 real sec...
sorting block 16, #reads = 1080422 mem = 536882838 peak = 963514368
[M::mem_process_seqs] Processed 674840 reads in 96.870 CPU sec, 3.040 real sec
sorting block 17, #reads = 1079159 mem = 536880462 peak = 995770368
[M::process] read 674382 sequences (100000235 bp) in 2.192 CPU sec, 2.237 real sec...
[M::process] read 674340 sequences (100000126 bp) in 1.470 CPU sec, 1.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280548, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 351, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (365.99, 159.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674446 reads in 81.698 CPU sec, 2.582 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280643, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 350, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (365.58, 160.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281009, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 350, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (365.45, 159.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674264 sequences (100000109 bp) in 1.617 CPU sec, 1.625 real sec...
[M::mem_process_seqs] Processed 674600 reads in 228.450 CPU sec, 7.235 real sec
sorting block 18, #reads = 1078112 mem = 536879817 peak = 995770368
[M::mem_process_seqs] Processed 674382 reads in 114.614 CPU sec, 3.658 real sec
sorting block 19, #reads = 1079314 mem = 536880377 peak = 995770368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281338, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (366.11, 159.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673904 sequences (100000006 bp) in 2.426 CPU sec, 2.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281289, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (366.79, 159.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674340 reads in 146.684 CPU sec, 4.633 real sec
[M::process] read 673764 sequences (100000034 bp) in 1.965 CPU sec, 1.969 real sec...
[M::mem_process_seqs] Processed 674264 reads in 98.628 CPU sec, 3.098 real sec
sorting block 20, #reads = 1079041 mem = 536878777 peak = 995770368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280673, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 354, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (368.96, 160.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673630 sequences (100000032 bp) in 2.544 CPU sec, 2.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280806, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 357, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (372.04, 162.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673904 reads in 144.843 CPU sec, 4.563 real sec
[M::process] read 673742 sequences (100000196 bp) in 1.968 CPU sec, 1.969 real sec...
[M::mem_process_seqs] Processed 673764 reads in 94.471 CPU sec, 2.968 real sec
sorting block 21, #reads = 1079516 mem = 536878900 peak = 995770368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280564, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 358, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (372.79, 162.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673522 sequences (100000135 bp) in 2.295 CPU sec, 2.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280964, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 359, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (373.56, 162.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673630 reads in 142.132 CPU sec, 4.494 real sec
[M::process] read 674850 sequences (100000031 bp) in 2.103 CPU sec, 2.117 real sec...
[M::mem_process_seqs] Processed 673742 reads in 95.659 CPU sec, 3.003 real sec
sorting block 22, #reads = 1079757 mem = 536880334 peak = 995770368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280701, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 361, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (376.03, 164.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678126 sequences (100000219 bp) in 2.370 CPU sec, 2.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281320, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (365.95, 160.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673522 reads in 145.672 CPU sec, 4.611 real sec
sorting block 23, #reads = 1078082 mem = 536880289 peak = 995770368
[M::process] read 677772 sequences (100000044 bp) in 2.094 CPU sec, 2.096 real sec...
[M::mem_process_seqs] Processed 674850 reads in 99.066 CPU sec, 3.104 real sec
sorting block 24, #reads = 1077729 mem = 536879266 peak = 1024069632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281692, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (347.88, 158.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677272 sequences (100000153 bp) in 2.139 CPU sec, 2.193 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281758, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (351.55, 160.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677444 sequences (100000151 bp) in 1.860 CPU sec, 1.865 real sec...
[M::mem_process_seqs] Processed 678126 reads in 150.181 CPU sec, 4.752 real sec
[M::mem_process_seqs] Processed 677772 reads in 98.938 CPU sec, 3.122 real sec
sorting block 25, #reads = 1083212 mem = 536877598 peak = 1024069632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281666, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 338, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (354.05, 160.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677058 sequences (100000117 bp) in 2.440 CPU sec, 2.496 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282290, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677182 sequences (100000129 bp) in 1.540 CPU sec, 1.536 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (230, 338, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (353.83, 160.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677272 reads in 150.156 CPU sec, 4.749 real sec
[M::mem_process_seqs] Processed 677444 reads in 101.429 CPU sec, 3.211 real sec
sorting block 26, #reads = 1081819 mem = 536880802 peak = 1024069632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281471, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 339, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (354.33, 159.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676884 sequences (100000198 bp) in 2.295 CPU sec, 2.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281803, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 340, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (354.87, 159.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676702 sequences (100000282 bp) in 2.000 CPU sec, 1.996 real sec...
[M::mem_process_seqs] Processed 677058 reads in 150.927 CPU sec, 4.764 real sec
sorting block 27, #reads = 1081285 mem = 536879232 peak = 1024069632
[M::mem_process_seqs] Processed 677182 reads in 100.345 CPU sec, 3.156 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281424, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 340, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (355.61, 159.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676730 sequences (100000017 bp) in 2.613 CPU sec, 2.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281668, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 344, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (358.57, 160.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675986 sequences (100000257 bp) in 1.990 CPU sec, 1.989 real sec...
[M::mem_process_seqs] Processed 676884 reads in 151.484 CPU sec, 4.770 real sec
sorting block 28, #reads = 1082498 mem = 536876240 peak = 1024069632
[M::mem_process_seqs] Processed 676702 reads in 101.459 CPU sec, 3.199 real sec
sorting block 29, #reads = 1080902 mem = 536879536 peak = 1054257152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281367, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 345, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (359.04, 160.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676034 sequences (100000152 bp) in 2.040 CPU sec, 2.086 real sec...
[M::process] read 675866 sequences (100000229 bp) in 1.490 CPU sec, 1.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281272, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (361.66, 161.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676730 reads in 146.595 CPU sec, 4.645 real sec
[M::mem_process_seqs] Processed 675986 reads in 97.666 CPU sec, 3.095 real sec
sorting block 30, #reads = 1081120 mem = 536884365 peak = 1054257152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281229, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 350, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (362.69, 160.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675650 sequences (100000280 bp) in 2.291 CPU sec, 2.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280788, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 350, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (364.81, 162.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675472 sequences (100000020 bp) in 1.610 CPU sec, 1.609 real sec...
[M::mem_process_seqs] Processed 676034 reads in 148.854 CPU sec, 4.714 real sec
[M::mem_process_seqs] Processed 675866 reads in 99.341 CPU sec, 3.138 real sec
sorting block 31, #reads = 1080552 mem = 536880367 peak = 1054257152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280794, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 354, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (368.17, 164.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675512 sequences (100000054 bp) in 2.156 CPU sec, 2.204 real sec...
[M::process] read 675260 sequences (100000242 bp) in 1.510 CPU sec, 1.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281061, 1, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (346, 346, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (62, 772)
[M::mem_pestat] mean and std.dev: (360.12, 92.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 354, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (367.76, 163.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675650 reads in 154.941 CPU sec, 4.911 real sec
sorting block 32, #reads = 1080017 mem = 536881453 peak = 1054257152
[M::mem_process_seqs] Processed 675472 reads in 102.881 CPU sec, 3.263 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281302, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 354, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (368.85, 164.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675430 sequences (100000242 bp) in 2.186 CPU sec, 2.226 real sec...
[M::process] read 675464 sequences (100000131 bp) in 1.490 CPU sec, 1.496 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281157, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 354, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (368.46, 163.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675512 reads in 149.989 CPU sec, 4.749 real sec
sorting block 33, #reads = 1080157 mem = 536877521 peak = 1054257152
[M::mem_process_seqs] Processed 675260 reads in 99.708 CPU sec, 3.169 real sec
sorting block 34, #reads = 1080763 mem = 536880512 peak = 1063059456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281315, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 355, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (369.56, 164.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675362 sequences (100000105 bp) in 2.229 CPU sec, 2.268 real sec...
[M::process] read 675308 sequences (100000076 bp) in 1.570 CPU sec, 1.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281553, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 353, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (368.10, 164.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675430 reads in 154.746 CPU sec, 4.905 real sec
[M::mem_process_seqs] Processed 675464 reads in 101.369 CPU sec, 3.216 real sec
sorting block 35, #reads = 1079869 mem = 536879801 peak = 1063059456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280972, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 352, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (367.85, 164.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675310 sequences (100000119 bp) in 1.961 CPU sec, 2.007 real sec...
[M::process] read 675580 sequences (100000063 bp) in 1.510 CPU sec, 1.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280732, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 352, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (367.44, 163.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675362 reads in 150.765 CPU sec, 4.784 real sec
[M::mem_process_seqs] Processed 675308 reads in 99.769 CPU sec, 3.172 real sec
sorting block 36, #reads = 1079917 mem = 536885474 peak = 1063059456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281102, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 349, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (364.71, 162.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675782 sequences (100000296 bp) in 2.068 CPU sec, 2.104 real sec...
[M::process] read 675736 sequences (100000157 bp) in 1.500 CPU sec, 1.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281668, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (360.69, 160.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675310 reads in 148.874 CPU sec, 4.720 real sec
sorting block 37, #reads = 1079594 mem = 536880761 peak = 1063059456
[M::mem_process_seqs] Processed 675580 reads in 98.168 CPU sec, 3.122 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281022, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 343, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (358.22, 158.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675532 sequences (100000174 bp) in 2.547 CPU sec, 2.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281200, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 342, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (357.94, 158.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675782 reads in 148.352 CPU sec, 4.680 real sec
sorting block 38, #reads = 1079600 mem = 536879618 peak = 1063059456
[M::process] read 675626 sequences (100000004 bp) in 2.068 CPU sec, 2.065 real sec...
[M::mem_process_seqs] Processed 675736 reads in 100.789 CPU sec, 3.164 real sec
sorting block 39, #reads = 1079286 mem = 536879887 peak = 1128570880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281106, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 342, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (357.90, 158.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675244 sequences (100000158 bp) in 2.372 CPU sec, 2.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281243, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 343, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (359.06, 159.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675056 sequences (100000166 bp) in 2.020 CPU sec, 2.012 real sec...
[M::mem_process_seqs] Processed 675532 reads in 153.470 CPU sec, 4.844 real sec
[M::mem_process_seqs] Processed 675626 reads in 102.597 CPU sec, 3.235 real sec
sorting block 40, #reads = 1078808 mem = 536879062 peak = 1128570880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280994, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (360.19, 158.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675232 sequences (100000171 bp) in 2.366 CPU sec, 2.414 real sec...
[M::process] read 675176 sequences (100000110 bp) in 1.520 CPU sec, 1.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281090, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 345, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (360.36, 158.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675244 reads in 157.143 CPU sec, 4.980 real sec
[M::mem_process_seqs] Processed 675056 reads in 104.482 CPU sec, 3.317 real sec
sorting block 41, #reads = 1079245 mem = 536878722 peak = 1128570880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281222, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 343, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (359.29, 158.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674918 sequences (100000235 bp) in 2.046 CPU sec, 2.104 real sec...
[M::process] read 674476 sequences (100000236 bp) in 1.500 CPU sec, 1.501 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281101, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 345, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (360.78, 159.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675232 reads in 154.001 CPU sec, 4.891 real sec
sorting block 42, #reads = 1080217 mem = 536880289 peak = 1128570880
[M::mem_process_seqs] Processed 675176 reads in 102.197 CPU sec, 3.246 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281566, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (363.97, 160.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674860 sequences (100000136 bp) in 2.687 CPU sec, 2.720 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281287, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (367.75, 162.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674918 reads in 154.031 CPU sec, 4.862 real sec
sorting block 43, #reads = 1078363 mem = 536878325 peak = 1128570880
[M::process] read 674572 sequences (100000088 bp) in 2.077 CPU sec, 2.078 real sec...
[M::mem_process_seqs] Processed 674476 reads in 100.629 CPU sec, 3.164 real sec
sorting block 44, #reads = 1079460 mem = 536883683 peak = 1140473856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281777, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (367.66, 162.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674414 sequences (100000263 bp) in 2.349 CPU sec, 2.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281245, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (367.12, 161.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674860 reads in 148.293 CPU sec, 4.689 real sec
[M::process] read 674452 sequences (100000106 bp) in 2.047 CPU sec, 2.041 real sec...
[M::mem_process_seqs] Processed 674572 reads in 100.099 CPU sec, 3.143 real sec
sorting block 45, #reads = 1079140 mem = 536877195 peak = 1140473856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281396, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 354, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (370.06, 163.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674200 sequences (100000206 bp) in 2.594 CPU sec, 2.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280991, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 353, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (368.67, 162.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674414 reads in 148.375 CPU sec, 4.674 real sec
[M::process] read 674320 sequences (100000088 bp) in 1.969 CPU sec, 1.976 real sec...
[M::mem_process_seqs] Processed 674452 reads in 99.332 CPU sec, 3.124 real sec
sorting block 46, #reads = 1079124 mem = 536878305 peak = 1140473856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281435, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 355, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (371.43, 164.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674264 sequences (100000215 bp) in 2.439 CPU sec, 2.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280872, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 356, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (372.24, 165.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674200 reads in 153.751 CPU sec, 4.863 real sec
[M::process] read 676942 sequences (100000075 bp) in 2.048 CPU sec, 2.057 real sec...
sorting block 47, #reads = 1078237 mem = 536878704 peak = 1140473856
[M::mem_process_seqs] Processed 674320 reads in 105.297 CPU sec, 3.310 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281424, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 360, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (376.26, 167.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678796 sequences (100000130 bp) in 2.306 CPU sec, 2.359 real sec...
[M::process] read 678194 sequences (100000226 bp) in 1.530 CPU sec, 1.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281295, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 341, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (356.52, 160.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674264 reads in 155.524 CPU sec, 4.925 real sec
sorting block 48, #reads = 1077636 mem = 536878340 peak = 1140473856
[M::mem_process_seqs] Processed 676942 reads in 103.217 CPU sec, 3.280 real sec
sorting block 49, #reads = 1077991 mem = 536883017 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282353, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (343.22, 155.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678514 sequences (100000099 bp) in 2.403 CPU sec, 2.437 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282351, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (346.77, 155.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678306 sequences (100000181 bp) in 1.930 CPU sec, 1.938 real sec...
[M::mem_process_seqs] Processed 678796 reads in 155.607 CPU sec, 4.919 real sec
[M::mem_process_seqs] Processed 678194 reads in 102.639 CPU sec, 3.231 real sec
sorting block 50, #reads = 1081882 mem = 536878881 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282201, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (344.95, 154.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677782 sequences (100000083 bp) in 2.461 CPU sec, 2.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281687, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (344.64, 153.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677346 sequences (100000026 bp) in 1.940 CPU sec, 1.936 real sec...
[M::mem_process_seqs] Processed 678514 reads in 157.834 CPU sec, 4.989 real sec
sorting block 51, #reads = 1081385 mem = 536886372 peak = 1183014912
[M::mem_process_seqs] Processed 678306 reads in 106.517 CPU sec, 3.353 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281767, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (347.64, 154.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677448 sequences (100000168 bp) in 2.414 CPU sec, 2.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282017, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (348.10, 153.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676822 sequences (100000081 bp) in 1.940 CPU sec, 1.944 real sec...
[M::mem_process_seqs] Processed 677782 reads in 162.025 CPU sec, 5.120 real sec
sorting block 52, #reads = 1080527 mem = 536878336 peak = 1183014912
[M::mem_process_seqs] Processed 677346 reads in 108.178 CPU sec, 3.424 real sec
sorting block 53, #reads = 1080875 mem = 536881573 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281789, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 676538 sequences (100000130 bp) in 2.190 CPU sec, 2.230 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (231, 338, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (351.10, 155.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676440 sequences (100000203 bp) in 1.580 CPU sec, 1.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281448, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 339, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (351.36, 153.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677448 reads in 162.640 CPU sec, 5.158 real sec
[M::mem_process_seqs] Processed 676822 reads in 109.644 CPU sec, 3.491 real sec
sorting block 54, #reads = 1080513 mem = 536879307 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281987, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 342, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (354.57, 155.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676112 sequences (100000079 bp) in 2.291 CPU sec, 2.322 real sec...
[M::process] read 675958 sequences (100000079 bp) in 1.480 CPU sec, 1.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281559, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 342, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (355.50, 156.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676538 reads in 160.847 CPU sec, 5.099 real sec
[M::mem_process_seqs] Processed 676440 reads in 106.814 CPU sec, 3.397 real sec
sorting block 55, #reads = 1080563 mem = 536880820 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281426, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 344, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (356.29, 155.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675786 sequences (100000043 bp) in 2.462 CPU sec, 2.499 real sec...
[M::process] read 675710 sequences (100000036 bp) in 1.610 CPU sec, 1.613 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281449, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 344, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (356.93, 156.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676112 reads in 155.827 CPU sec, 4.935 real sec
[M::mem_process_seqs] Processed 675958 reads in 103.614 CPU sec, 3.279 real sec
sorting block 56, #reads = 1080965 mem = 536877131 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280979, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (359.60, 157.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675726 sequences (100000210 bp) in 1.997 CPU sec, 2.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280791, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (360.24, 157.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675704 sequences (100000280 bp) in 1.940 CPU sec, 1.937 real sec...
[M::mem_process_seqs] Processed 675786 reads in 153.200 CPU sec, 4.849 real sec
sorting block 57, #reads = 1080169 mem = 536878032 peak = 1183014912
[M::mem_process_seqs] Processed 675710 reads in 101.122 CPU sec, 3.193 real sec
sorting block 58, #reads = 1080019 mem = 536881988 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281818, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (359.62, 157.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675584 sequences (100000064 bp) in 2.593 CPU sec, 2.622 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281247, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (360.49, 158.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675382 sequences (100000127 bp) in 2.010 CPU sec, 2.012 real sec...
[M::mem_process_seqs] Processed 675726 reads in 154.786 CPU sec, 4.883 real sec
[M::mem_process_seqs] Processed 675704 reads in 103.605 CPU sec, 3.263 real sec
sorting block 59, #reads = 1080163 mem = 536878816 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281085, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (363.79, 159.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675476 sequences (100000187 bp) in 2.435 CPU sec, 2.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281562, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (362.89, 158.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676008 sequences (100000030 bp) in 2.010 CPU sec, 2.003 real sec...
[M::mem_process_seqs] Processed 675584 reads in 156.916 CPU sec, 4.955 real sec
[M::mem_process_seqs] Processed 675382 reads in 102.611 CPU sec, 3.235 real sec
sorting block 60, #reads = 1080267 mem = 536880019 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281008, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 349, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (363.32, 159.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675576 sequences (100000285 bp) in 2.656 CPU sec, 2.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281577, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 341, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (356.51, 157.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675476 reads in 151.517 CPU sec, 4.779 real sec
[M::process] read 675196 sequences (100000295 bp) in 2.078 CPU sec, 2.081 real sec...
[M::mem_process_seqs] Processed 676008 reads in 100.209 CPU sec, 3.151 real sec
sorting block 61, #reads = 1079793 mem = 536880241 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281513, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 343, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (358.45, 157.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675528 sequences (100000086 bp) in 2.383 CPU sec, 2.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281235, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 344, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (359.37, 157.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675576 reads in 154.071 CPU sec, 4.867 real sec
[M::process] read 675540 sequences (100000104 bp) in 2.000 CPU sec, 2.000 real sec...
sorting block 62, #reads = 1080562 mem = 536878001 peak = 1183014912
[M::mem_process_seqs] Processed 675196 reads in 101.994 CPU sec, 3.209 real sec
sorting block 63, #reads = 1078919 mem = 536878064 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280705, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 342, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (357.22, 156.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675352 sequences (100000077 bp) in 2.691 CPU sec, 2.736 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281412, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 343, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (357.75, 156.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675506 sequences (100000153 bp) in 2.050 CPU sec, 2.052 real sec...
[M::mem_process_seqs] Processed 675528 reads in 158.292 CPU sec, 4.989 real sec
[M::mem_process_seqs] Processed 675540 reads in 106.140 CPU sec, 3.346 real sec
sorting block 64, #reads = 1079164 mem = 536878510 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281309, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 344, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (359.23, 157.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675326 sequences (100000263 bp) in 2.424 CPU sec, 2.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280885, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 343, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (358.63, 158.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675362 sequences (100000174 bp) in 2.040 CPU sec, 2.037 real sec...
[M::mem_process_seqs] Processed 675352 reads in 161.375 CPU sec, 5.100 real sec
[M::mem_process_seqs] Processed 675506 reads in 108.429 CPU sec, 3.417 real sec
sorting block 65, #reads = 1079582 mem = 536877794 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280314, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (361.56, 158.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675168 sequences (100000143 bp) in 2.652 CPU sec, 2.682 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281370, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 343, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (357.81, 156.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675158 sequences (100000275 bp) in 2.020 CPU sec, 2.020 real sec...
[M::mem_process_seqs] Processed 675326 reads in 162.753 CPU sec, 5.136 real sec
sorting block 66, #reads = 1077891 mem = 536879473 peak = 1183014912
[M::mem_process_seqs] Processed 675362 reads in 107.723 CPU sec, 3.400 real sec
[M::process] read 674958 sequences (100000039 bp) in 1.990 CPU sec, 2.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281202, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 343, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (357.31, 155.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674864 sequences (100000114 bp) in 1.570 CPU sec, 1.574 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281103, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 345, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (359.93, 157.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (315, 464, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (17, 762)
[M::mem_pestat] mean and std.dev: (393.80, 132.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675168 reads in 156.222 CPU sec, 4.952 real sec
sorting block 67, #reads = 1077723 mem = 536880594 peak = 1183014912
[M::mem_process_seqs] Processed 675158 reads in 103.606 CPU sec, 3.302 real sec
sorting block 68, #reads = 1078502 mem = 536878503 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280999, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (363.70, 159.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (9046, 9046, 9046)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (9046, 9046)
[M::mem_pestat] mean and std.dev: (9046.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (9046, 9046)
[M::mem_pestat] skip orientation RR
[M::process] read 674744 sequences (100000285 bp) in 2.638 CPU sec, 2.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281353, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (364.67, 161.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674786 sequences (100000254 bp) in 1.980 CPU sec, 1.982 real sec...
[M::mem_process_seqs] Processed 674958 reads in 159.702 CPU sec, 5.034 real sec
[M::mem_process_seqs] Processed 674864 reads in 107.339 CPU sec, 3.392 real sec
sorting block 69, #reads = 1077944 mem = 536886089 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281621, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 350, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (365.81, 161.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674972 sequences (100000116 bp) in 2.468 CPU sec, 2.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280527, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (367.75, 162.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674668 sequences (100000110 bp) in 1.980 CPU sec, 1.982 real sec...
[M::mem_process_seqs] Processed 674744 reads in 157.114 CPU sec, 4.958 real sec
[M::mem_process_seqs] Processed 674786 reads in 104.279 CPU sec, 3.284 real sec
sorting block 70, #reads = 1078054 mem = 536881772 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281213, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (365.27, 162.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674718 sequences (100000138 bp) in 2.608 CPU sec, 2.644 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280810, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (368.58, 164.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674972 reads in 154.426 CPU sec, 4.867 real sec
[M::process] read 674736 sequences (100000285 bp) in 2.060 CPU sec, 2.052 real sec...
[M::mem_process_seqs] Processed 674668 reads in 103.761 CPU sec, 3.262 real sec
sorting block 71, #reads = 1079076 mem = 536880880 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281392, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (367.74, 164.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678494 sequences (100000059 bp) in 2.386 CPU sec, 2.415 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281145, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (366.90, 162.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677844 sequences (100000165 bp) in 1.940 CPU sec, 1.941 real sec...
[M::mem_process_seqs] Processed 674718 reads in 156.974 CPU sec, 4.961 real sec
sorting block 72, #reads = 1077646 mem = 536879641 peak = 1183014912
[M::mem_process_seqs] Processed 674736 reads in 107.814 CPU sec, 3.399 real sec
sorting block 73, #reads = 1076295 mem = 536878289 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 281985, 2, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (568, 568, 568)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (568, 568)
[M::mem_pestat] mean and std.dev: (568.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (568, 568)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (343.50, 153.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 677834 sequences (100000081 bp) in 2.758 CPU sec, 2.796 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282000, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (347.86, 155.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677676 sequences (100000288 bp) in 1.950 CPU sec, 1.950 real sec...
[M::mem_process_seqs] Processed 678494 reads in 156.551 CPU sec, 4.929 real sec
[M::mem_process_seqs] Processed 677844 reads in 173.939 CPU sec, 5.497 real sec
sorting block 74, #reads = 1080585 mem = 536880537 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282095, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (349.57, 156.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677422 sequences (100000179 bp) in 2.390 CPU sec, 2.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281767, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (349.87, 156.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677834 reads in 149.566 CPU sec, 4.724 real sec
[M::process] read 677088 sequences (100000174 bp) in 1.989 CPU sec, 1.993 real sec...
[M::mem_process_seqs] Processed 677676 reads in 99.625 CPU sec, 3.132 real sec
sorting block 75, #reads = 1081860 mem = 536879565 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281422, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (350.63, 156.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677104 sequences (100000102 bp) in 2.688 CPU sec, 2.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281383, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 338, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (352.81, 157.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677008 sequences (100000079 bp) in 1.960 CPU sec, 1.963 real sec...
[M::mem_process_seqs] Processed 677422 reads in 155.784 CPU sec, 4.911 real sec
sorting block 76, #reads = 1081705 mem = 536877699 peak = 1183014912
[M::mem_process_seqs] Processed 677088 reads in 102.838 CPU sec, 3.238 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281500, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 340, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (353.92, 157.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676884 sequences (100000265 bp) in 2.403 CPU sec, 2.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281218, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 339, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (352.84, 156.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676460 sequences (100000024 bp) in 1.990 CPU sec, 1.988 real sec...
[M::mem_process_seqs] Processed 677104 reads in 153.639 CPU sec, 4.848 real sec
sorting block 77, #reads = 1081351 mem = 536876954 peak = 1183014912
[M::mem_process_seqs] Processed 677008 reads in 101.603 CPU sec, 3.207 real sec
sorting block 78, #reads = 1080725 mem = 536878845 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281716, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 341, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (355.04, 157.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676338 sequences (100000243 bp) in 2.624 CPU sec, 2.640 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281024, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 344, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (357.24, 157.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676884 reads in 153.331 CPU sec, 4.836 real sec
[M::process] read 676074 sequences (100000290 bp) in 1.970 CPU sec, 1.972 real sec...
[M::mem_process_seqs] Processed 676460 reads in 102.883 CPU sec, 3.240 real sec
sorting block 79, #reads = 1081089 mem = 536878459 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281462, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (359.68, 159.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (336, 336, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (336, 336)
[M::mem_pestat] mean and std.dev: (336.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (336, 336)
[M::mem_pestat] skip orientation RR
[M::process] read 675882 sequences (100000085 bp) in 2.393 CPU sec, 2.414 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281048, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (360.58, 158.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676030 sequences (100000247 bp) in 1.920 CPU sec, 1.921 real sec...
[M::mem_process_seqs] Processed 676338 reads in 151.584 CPU sec, 4.787 real sec
[M::mem_process_seqs] Processed 676074 reads in 99.677 CPU sec, 3.135 real sec
sorting block 80, #reads = 1080683 mem = 536876762 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280835, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (358.68, 157.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675908 sequences (100000056 bp) in 2.441 CPU sec, 2.479 real sec...
[M::process] read 675546 sequences (100000083 bp) in 1.590 CPU sec, 1.588 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281194, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (359.58, 157.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675882 reads in 157.348 CPU sec, 4.985 real sec
sorting block 81, #reads = 1079832 mem = 536879338 peak = 1183014912
[M::mem_process_seqs] Processed 676030 reads in 103.169 CPU sec, 3.273 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281231, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (360.76, 158.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675590 sequences (100000138 bp) in 2.372 CPU sec, 2.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281128, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (361.96, 158.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675638 sequences (100000279 bp) in 1.990 CPU sec, 1.992 real sec...
[M::mem_process_seqs] Processed 675908 reads in 155.718 CPU sec, 4.912 real sec
sorting block 82, #reads = 1080178 mem = 536879262 peak = 1183014912
[M::mem_process_seqs] Processed 675546 reads in 103.799 CPU sec, 3.276 real sec
sorting block 83, #reads = 1080126 mem = 536881202 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281493, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (359.82, 156.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675752 sequences (100000104 bp) in 2.466 CPU sec, 2.483 real sec...
[M::process] read 676252 sequences (100000067 bp) in 1.780 CPU sec, 1.787 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281163, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 349, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (362.17, 158.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675590 reads in 159.390 CPU sec, 5.044 real sec
[M::mem_process_seqs] Processed 675638 reads in 104.569 CPU sec, 3.310 real sec
sorting block 84, #reads = 1080418 mem = 536878644 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280819, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 349, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (362.84, 159.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677328 sequences (100000084 bp) in 2.128 CPU sec, 2.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280772, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (359.18, 159.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676022 sequences (100000196 bp) in 2.040 CPU sec, 2.033 real sec...
[M::mem_process_seqs] Processed 675752 reads in 154.959 CPU sec, 4.897 real sec
sorting block 85, #reads = 1078761 mem = 536877062 peak = 1183014912
[M::mem_process_seqs] Processed 676252 reads in 102.512 CPU sec, 3.246 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281735, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (348.75, 157.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675972 sequences (100000143 bp) in 2.631 CPU sec, 2.655 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280816, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 340, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (354.99, 156.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675884 sequences (100000126 bp) in 2.000 CPU sec, 1.996 real sec...
[M::mem_process_seqs] Processed 677328 reads in 155.467 CPU sec, 4.896 real sec
sorting block 86, #reads = 1079464 mem = 536881113 peak = 1183014912
[M::mem_process_seqs] Processed 676022 reads in 173.262 CPU sec, 5.478 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281379, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 339, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (353.75, 155.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675856 sequences (100000148 bp) in 2.432 CPU sec, 2.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281220, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 340, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (353.95, 155.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675640 sequences (100000221 bp) in 1.950 CPU sec, 1.950 real sec...
[M::mem_process_seqs] Processed 675972 reads in 155.341 CPU sec, 4.910 real sec
sorting block 87, #reads = 1078382 mem = 536878839 peak = 1183014912
[M::mem_process_seqs] Processed 675884 reads in 101.312 CPU sec, 3.185 real sec
sorting block 88, #reads = 1078222 mem = 536878420 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280970, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 339, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (352.92, 153.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675678 sequences (100000103 bp) in 2.569 CPU sec, 2.580 real sec...
[M::process] read 675586 sequences (100000282 bp) in 1.540 CPU sec, 1.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280719, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 338, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (352.04, 153.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675856 reads in 154.959 CPU sec, 4.902 real sec
[M::mem_process_seqs] Processed 675640 reads in 102.339 CPU sec, 3.243 real sec
sorting block 89, #reads = 1078403 mem = 536879696 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280876, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 339, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (352.90, 153.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675492 sequences (100000247 bp) in 2.387 CPU sec, 2.399 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280871, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 340, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (353.40, 153.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675452 sequences (100000018 bp) in 2.010 CPU sec, 2.012 real sec...
[M::mem_process_seqs] Processed 675678 reads in 154.605 CPU sec, 4.881 real sec
[M::mem_process_seqs] Processed 675586 reads in 102.269 CPU sec, 3.221 real sec
sorting block 90, #reads = 1078691 mem = 536884976 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280750, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 337, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (352.02, 153.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675462 sequences (100000096 bp) in 2.448 CPU sec, 2.463 real sec...
[M::process] read 675108 sequences (100000195 bp) in 1.500 CPU sec, 1.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280584, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 337, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (351.34, 151.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675492 reads in 154.168 CPU sec, 4.886 real sec
[M::mem_process_seqs] Processed 675452 reads in 101.175 CPU sec, 3.203 real sec
sorting block 91, #reads = 1078252 mem = 536883822 peak = 1183014912
sorting block 92, #reads = 1078448 mem = 536879101 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281010, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 335, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (349.64, 150.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1091)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674858 sequences (100000162 bp) in 2.010 CPU sec, 2.035 real sec...
[M::process] read 675042 sequences (100000035 bp) in 1.510 CPU sec, 1.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280838, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 341, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (354.37, 153.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675462 reads in 152.246 CPU sec, 4.836 real sec
[M::mem_process_seqs] Processed 675108 reads in 99.978 CPU sec, 3.169 real sec
sorting block 93, #reads = 1078259 mem = 536880304 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281247, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 344, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (358.58, 155.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674880 sequences (100000167 bp) in 2.488 CPU sec, 2.501 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281070, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 346, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (361.02, 157.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674806 sequences (100000277 bp) in 1.520 CPU sec, 1.518 real sec...
[M::mem_process_seqs] Processed 674858 reads in 152.093 CPU sec, 4.814 real sec
[M::mem_process_seqs] Processed 675042 reads in 101.191 CPU sec, 3.203 real sec
sorting block 94, #reads = 1079303 mem = 536878145 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281097, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (362.99, 159.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674780 sequences (100000046 bp) in 2.201 CPU sec, 2.232 real sec...
[M::process] read 674450 sequences (100000138 bp) in 1.490 CPU sec, 1.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280873, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (363.48, 159.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674880 reads in 153.215 CPU sec, 4.858 real sec
[M::mem_process_seqs] Processed 674806 reads in 100.418 CPU sec, 3.189 real sec
sorting block 95, #reads = 1078334 mem = 536880269 peak = 1183014912
[M::process] read 678902 sequences (100000084 bp) in 2.490 CPU sec, 2.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281418, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 354, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (368.98, 161.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681438 sequences (100000286 bp) in 2.070 CPU sec, 2.077 real sec...
[M::mem_process_seqs] Processed 674450 reads in 88.401 CPU sec, 2.772 real sec
sorting block 96, #reads = 1077916 mem = 536879973 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281597, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (366.66, 161.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282921, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (342.42, 159.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674780 reads in 244.983 CPU sec, 7.736 real sec
[M::process] read 680752 sequences (100000058 bp) in 2.019 CPU sec, 2.024 real sec...
[M::mem_process_seqs] Processed 678902 reads in 128.188 CPU sec, 4.058 real sec
sorting block 97, #reads = 1078644 mem = 536878925 peak = 1183014912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284140, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 305, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (328.82, 156.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679760 sequences (100000291 bp) in 2.696 CPU sec, 2.733 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282730, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 313, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (334.56, 157.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678686 sequences (100000043 bp) in 1.990 CPU sec, 1.981 real sec...
[M::mem_process_seqs] Processed 681438 reads in 166.399 CPU sec, 5.259 real sec
sorting block 98, #reads = 1077951 mem = 536879872 peak = 1183014912
[M::mem_process_seqs] Processed 680752 reads in 109.116 CPU sec, 3.445 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282743, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 321, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (340.72, 159.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678276 sequences (100000193 bp) in 2.751 CPU sec, 2.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282537, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 328, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (348.01, 162.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679760 reads in 157.787 CPU sec, 4.975 real sec
[M::process] read 678052 sequences (100000027 bp) in 2.010 CPU sec, 2.005 real sec...
sorting block 99, #reads = 1079273 mem = 536883321 peak = 1183014912
[M::mem_process_seqs] Processed 678686 reads in 101.624 CPU sec, 3.199 real sec
sorting block 100, #reads = 1078906 mem = 536879450 peak = 1210142720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282096, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (350.38, 162.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677636 sequences (100000261 bp) in 2.255 CPU sec, 2.317 real sec...
[M::process] read 677352 sequences (100000171 bp) in 1.590 CPU sec, 1.582 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282261, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (353.04, 163.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678276 reads in 153.864 CPU sec, 4.876 real sec
[M::mem_process_seqs] Processed 678052 reads in 103.532 CPU sec, 3.277 real sec
sorting block 101, #reads = 1080454 mem = 536880971 peak = 1210142720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282429, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (355.96, 163.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677080 sequences (100000022 bp) in 2.563 CPU sec, 2.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282250, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (355.57, 162.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677210 sequences (100000084 bp) in 1.970 CPU sec, 1.973 real sec...
[M::mem_process_seqs] Processed 677636 reads in 160.777 CPU sec, 5.072 real sec
[M::mem_process_seqs] Processed 677352 reads in 107.226 CPU sec, 3.385 real sec
sorting block 102, #reads = 1079524 mem = 536880671 peak = 1210142720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281958, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 341, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (358.13, 163.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676588 sequences (100000143 bp) in 2.199 CPU sec, 2.245 real sec...
[M::process] read 676546 sequences (100000202 bp) in 1.530 CPU sec, 1.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282217, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 342, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (357.87, 162.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677080 reads in 159.508 CPU sec, 5.059 real sec
sorting block 103, #reads = 1078950 mem = 536878268 peak = 1210142720
[M::mem_process_seqs] Processed 677210 reads in 105.144 CPU sec, 3.343 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281836, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 345, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (361.08, 163.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676424 sequences (100000168 bp) in 2.775 CPU sec, 2.827 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281325, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 345, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (360.92, 163.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676132 sequences (100000097 bp) in 2.040 CPU sec, 2.035 real sec...
[M::mem_process_seqs] Processed 676588 reads in 158.777 CPU sec, 4.996 real sec
sorting block 104, #reads = 1079506 mem = 536878185 peak = 1210142720
[M::mem_process_seqs] Processed 676546 reads in 176.323 CPU sec, 5.568 real sec
sorting block 105, #reads = 1078166 mem = 536881856 peak = 1210142720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282037, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 349, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (364.29, 164.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676290 sequences (100000288 bp) in 2.280 CPU sec, 2.316 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282222, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 347, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (362.76, 162.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675804 sequences (100000131 bp) in 2.070 CPU sec, 2.069 real sec...
[M::mem_process_seqs] Processed 676424 reads in 154.570 CPU sec, 4.882 real sec
[M::mem_process_seqs] Processed 676132 reads in 102.532 CPU sec, 3.231 real sec
sorting block 106, #reads = 1078503 mem = 536878523 peak = 1210142720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281682, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 345, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (359.78, 160.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675996 sequences (100000070 bp) in 2.628 CPU sec, 2.664 real sec...
[M::process] read 675714 sequences (100000062 bp) in 1.510 CPU sec, 1.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281705, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 348, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (362.95, 162.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676290 reads in 160.281 CPU sec, 5.069 real sec
[M::mem_process_seqs] Processed 675804 reads in 105.366 CPU sec, 3.337 real sec
sorting block 107, #reads = 1076661 mem = 536880424 peak = 1210142720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281502, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 347, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (361.78, 161.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675824 sequences (100000212 bp) in 2.258 CPU sec, 2.290 real sec...
[M::process] read 675768 sequences (100000176 bp) in 1.550 CPU sec, 1.554 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281707, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (361.80, 160.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675996 reads in 158.189 CPU sec, 5.012 real sec
sorting block 108, #reads = 1076672 mem = 536882261 peak = 1210142720
[M::mem_process_seqs] Processed 675714 reads in 104.107 CPU sec, 3.308 real sec
sorting block 109, #reads = 1076541 mem = 536880365 peak = 1210142720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281156, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (362.37, 161.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675906 sequences (100000009 bp) in 2.271 CPU sec, 2.315 real sec...
[M::process] read 675988 sequences (100000172 bp) in 1.520 CPU sec, 1.519 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281645, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (361.86, 160.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675824 reads in 160.204 CPU sec, 5.078 real sec
[M::mem_process_seqs] Processed 675768 reads in 105.542 CPU sec, 3.358 real sec
sorting block 110, #reads = 1076616 mem = 536881028 peak = 1210142720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281510, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (361.32, 160.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675496 sequences (100000139 bp) in 2.209 CPU sec, 2.252 real sec...
[M::process] read 675536 sequences (100000150 bp) in 1.530 CPU sec, 1.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282160, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 343, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (358.06, 159.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675906 reads in 157.761 CPU sec, 5.003 real sec
[M::mem_process_seqs] Processed 675988 reads in 104.613 CPU sec, 3.326 real sec
sorting block 111, #reads = 1077035 mem = 536883627 peak = 1210142720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281677, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (359.93, 159.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675512 sequences (100000186 bp) in 2.182 CPU sec, 2.226 real sec...
[M::process] read 675558 sequences (100000283 bp) in 1.510 CPU sec, 1.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281596, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (361.35, 160.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675496 reads in 159.650 CPU sec, 5.065 real sec
[M::mem_process_seqs] Processed 675536 reads in 104.287 CPU sec, 3.307 real sec
sorting block 112, #reads = 1075469 mem = 536879933 peak = 1210142720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281538, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (361.50, 160.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675532 sequences (100000223 bp) in 2.485 CPU sec, 2.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281615, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (360.12, 159.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675234 sequences (100000156 bp) in 1.700 CPU sec, 1.698 real sec...
[M::mem_process_seqs] Processed 675512 reads in 160.148 CPU sec, 5.065 real sec
sorting block 113, #reads = 1076163 mem = 536880471 peak = 1210142720
[M::mem_process_seqs] Processed 675558 reads in 106.223 CPU sec, 3.362 real sec
sorting block 114, #reads = 1076651 mem = 536878764 peak = 1210142720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281424, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 347, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (361.80, 159.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675542 sequences (100000224 bp) in 2.639 CPU sec, 2.674 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281639, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 344, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (358.79, 157.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675234 sequences (100000059 bp) in 1.950 CPU sec, 1.945 real sec...
[M::mem_process_seqs] Processed 675234 reads in 160.251 CPU sec, 5.049 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281732, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 343, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (358.99, 158.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675168 sequences (100000299 bp) in 2.267 CPU sec, 2.268 real sec...
[M::mem_process_seqs] Processed 675542 reads in 89.957 CPU sec, 2.836 real sec
sorting block 115, #reads = 1076390 mem = 536883335 peak = 1210142720
[M::mem_process_seqs] Processed 675532 reads in 283.710 CPU sec, 8.973 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281903, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (361.04, 158.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675228 sequences (100000074 bp) in 2.533 CPU sec, 2.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281066, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (361.56, 159.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674926 sequences (100000139 bp) in 1.490 CPU sec, 1.493 real sec...
[M::mem_process_seqs] Processed 675234 reads in 178.571 CPU sec, 5.652 real sec
sorting block 116, #reads = 1076359 mem = 536881443 peak = 1210142720
[M::mem_process_seqs] Processed 675168 reads in 107.113 CPU sec, 3.390 real sec
sorting block 117, #reads = 1075282 mem = 536878655 peak = 1230295040
[M::process] read 674898 sequences (100000268 bp) in 1.970 CPU sec, 2.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281344, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (362.27, 160.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674850 sequences (100000163 bp) in 1.570 CPU sec, 1.568 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281494, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (366.99, 162.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282043, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (365.89, 162.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675228 reads in 226.846 CPU sec, 7.229 real sec
[M::mem_process_seqs] Processed 674898 reads in 172.469 CPU sec, 5.523 real sec
[M::mem_process_seqs] Processed 674926 reads in 190.766 CPU sec, 6.119 real sec
sorting block 118, #reads = 1075074 mem = 536880752 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281850, 1, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (367.34, 163.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (371, 1133, 1184)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2810)
[M::mem_pestat] mean and std.dev: (948.57, 419.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3623)
[M::mem_pestat] skip orientation RR
sorting block 119, #reads = 1075552 mem = 536878630 peak = 1230295040
[M::process] read 675638 sequences (100000145 bp) in 3.020 CPU sec, 3.131 real sec...
[M::mem_process_seqs] Processed 674850 reads in 93.742 CPU sec, 3.066 real sec
[M::process] read 683998 sequences (100000247 bp) in 2.174 CPU sec, 2.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282189, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (364.29, 164.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683208 sequences (100000301 bp) in 1.530 CPU sec, 1.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284426, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 290, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (312.43, 147.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683062 sequences (100000064 bp) in 1.530 CPU sec, 1.531 real sec...
[M::mem_process_seqs] Processed 675638 reads in 171.456 CPU sec, 5.389 real sec
sorting block 120, #reads = 1075653 mem = 536881493 peak = 1230295040
[M::mem_process_seqs] Processed 683998 reads in 113.310 CPU sec, 3.583 real sec
sorting block 121, #reads = 1076193 mem = 536879697 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284594, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 296, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (317.36, 149.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682242 sequences (100000279 bp) in 2.691 CPU sec, 2.731 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283703, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 296, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (315.79, 146.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1052)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681194 sequences (100000067 bp) in 2.020 CPU sec, 2.023 real sec...
[M::mem_process_seqs] Processed 683208 reads in 167.949 CPU sec, 5.298 real sec
[M::mem_process_seqs] Processed 683062 reads in 111.952 CPU sec, 3.537 real sec
sorting block 122, #reads = 1077244 mem = 536875554 peak = 1230295040
[M::process] read 680210 sequences (100000015 bp) in 2.210 CPU sec, 2.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283795, 4, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (307, 307, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (307, 307)
[M::mem_pestat] mean and std.dev: (307.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (307, 307)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 301, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (319.40, 147.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 680076 sequences (100000061 bp) in 1.530 CPU sec, 1.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283596, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 307, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (325.22, 148.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682242 reads in 170.195 CPU sec, 5.394 real sec
[M::mem_process_seqs] Processed 681194 reads in 111.421 CPU sec, 3.559 real sec
sorting block 123, #reads = 1074432 mem = 536882867 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282917, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 314, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (330.36, 150.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679934 sequences (100000278 bp) in 2.298 CPU sec, 2.348 real sec...
[M::process] read 679274 sequences (100000170 bp) in 2.040 CPU sec, 2.043 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283254, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 315, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (331.97, 150.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680210 reads in 170.070 CPU sec, 5.570 real sec
[M::mem_process_seqs] Processed 680076 reads in 109.508 CPU sec, 3.469 real sec
sorting block 124, #reads = 1076133 mem = 536878817 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283557, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 318, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (335.40, 153.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678790 sequences (100000060 bp) in 2.513 CPU sec, 2.544 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282785, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 323, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (339.24, 154.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679058 sequences (100000275 bp) in 2.030 CPU sec, 2.031 real sec...
[M::mem_process_seqs] Processed 679934 reads in 166.612 CPU sec, 5.258 real sec
sorting block 125, #reads = 1075829 mem = 536876405 peak = 1230295040
[M::mem_process_seqs] Processed 679274 reads in 109.571 CPU sec, 3.465 real sec
sorting block 126, #reads = 1074558 mem = 536878887 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283403, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 327, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (342.33, 154.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678616 sequences (100000247 bp) in 2.687 CPU sec, 2.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282944, 3, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (844, 1800, 1800)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3712)
[M::mem_pestat] mean and std.dev: (1410.30, 614.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4668)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 326, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (341.09, 154.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 678612 sequences (100000087 bp) in 2.010 CPU sec, 2.007 real sec...
[M::mem_process_seqs] Processed 678790 reads in 164.928 CPU sec, 5.205 real sec
[M::mem_process_seqs] Processed 679058 reads in 109.090 CPU sec, 3.436 real sec
sorting block 127, #reads = 1076363 mem = 536878865 peak = 1230295040
[M::process] read 677596 sequences (100000034 bp) in 2.346 CPU sec, 2.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283346, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (342.91, 153.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677454 sequences (100000128 bp) in 2.060 CPU sec, 2.062 real sec...
[M::mem_process_seqs] Processed 678612 reads in 87.461 CPU sec, 2.750 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282982, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 327, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (341.89, 154.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 128, #reads = 1075968 mem = 536877239 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282503, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 334, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (348.56, 155.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678616 reads in 247.822 CPU sec, 7.824 real sec
[M::process] read 677264 sequences (100000027 bp) in 2.101 CPU sec, 2.108 real sec...
[M::mem_process_seqs] Processed 677596 reads in 123.126 CPU sec, 3.909 real sec
sorting block 129, #reads = 1076389 mem = 536879068 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282017, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 337, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (351.14, 156.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676842 sequences (100000135 bp) in 2.555 CPU sec, 2.614 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282210, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (354.06, 158.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676562 sequences (100000081 bp) in 1.980 CPU sec, 1.974 real sec...
[M::mem_process_seqs] Processed 677454 reads in 163.157 CPU sec, 5.153 real sec
[M::mem_process_seqs] Processed 677264 reads in 109.875 CPU sec, 3.464 real sec
sorting block 130, #reads = 1074273 mem = 536879131 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282261, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 341, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (355.33, 157.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676542 sequences (100000010 bp) in 2.704 CPU sec, 2.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282679, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 341, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (355.61, 158.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675976 sequences (100000177 bp) in 2.010 CPU sec, 2.011 real sec...
[M::mem_process_seqs] Processed 676842 reads in 163.017 CPU sec, 5.134 real sec
sorting block 131, #reads = 1075088 mem = 536878134 peak = 1230295040
[M::mem_process_seqs] Processed 676562 reads in 107.706 CPU sec, 3.396 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281898, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 340, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (355.01, 158.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676318 sequences (100000053 bp) in 2.725 CPU sec, 2.767 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281880, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 342, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (356.95, 158.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676078 sequences (100000059 bp) in 2.030 CPU sec, 2.031 real sec...
[M::mem_process_seqs] Processed 676542 reads in 162.099 CPU sec, 5.106 real sec
sorting block 132, #reads = 1074913 mem = 536878533 peak = 1230295040
[M::mem_process_seqs] Processed 675976 reads in 105.220 CPU sec, 3.315 real sec
sorting block 133, #reads = 1075013 mem = 536880038 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282299, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 341, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (355.69, 157.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675894 sequences (100000116 bp) in 2.675 CPU sec, 2.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281449, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 342, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (357.50, 158.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675836 sequences (100000214 bp) in 2.050 CPU sec, 2.049 real sec...
[M::mem_process_seqs] Processed 676318 reads in 160.485 CPU sec, 5.055 real sec
[M::mem_process_seqs] Processed 676078 reads in 107.521 CPU sec, 3.391 real sec
sorting block 134, #reads = 1075276 mem = 536885297 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282213, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 343, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (359.17, 160.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675648 sequences (100000180 bp) in 2.643 CPU sec, 2.667 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281648, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (360.18, 160.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675900 sequences (100000279 bp) in 2.080 CPU sec, 2.085 real sec...
[M::mem_process_seqs] Processed 675894 reads in 158.912 CPU sec, 5.004 real sec
[M::mem_process_seqs] Processed 675836 reads in 105.816 CPU sec, 3.346 real sec
sorting block 135, #reads = 1076116 mem = 536878939 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282291, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 343, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (359.37, 159.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675934 sequences (100000049 bp) in 2.665 CPU sec, 2.707 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281764, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 342, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (357.84, 159.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675210 sequences (100000016 bp) in 1.990 CPU sec, 1.989 real sec...
[M::mem_process_seqs] Processed 675648 reads in 160.885 CPU sec, 5.071 real sec
[M::mem_process_seqs] Processed 675900 reads in 107.252 CPU sec, 3.380 real sec
sorting block 136, #reads = 1075869 mem = 536879901 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281282, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 342, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (357.25, 158.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675016 sequences (100000204 bp) in 2.484 CPU sec, 2.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281163, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (363.13, 160.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675040 sequences (100000087 bp) in 2.040 CPU sec, 2.041 real sec...
[M::mem_process_seqs] Processed 675934 reads in 162.163 CPU sec, 5.118 real sec
sorting block 137, #reads = 1075182 mem = 536881764 peak = 1230295040
[M::mem_process_seqs] Processed 675210 reads in 107.144 CPU sec, 3.381 real sec
sorting block 138, #reads = 1075068 mem = 536877192 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281232, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (362.70, 160.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675194 sequences (100000048 bp) in 2.008 CPU sec, 2.060 real sec...
[M::process] read 674918 sequences (100000006 bp) in 1.520 CPU sec, 1.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281395, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (364.09, 161.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675016 reads in 155.023 CPU sec, 4.920 real sec
[M::mem_process_seqs] Processed 675040 reads in 102.864 CPU sec, 3.267 real sec
sorting block 139, #reads = 1076048 mem = 536880866 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281899, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 349, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (365.43, 162.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675822 sequences (100000179 bp) in 2.197 CPU sec, 2.227 real sec...
[M::process] read 678864 sequences (100000010 bp) in 1.590 CPU sec, 1.588 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281187, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 351, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (367.49, 163.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675194 reads in 156.682 CPU sec, 4.966 real sec
[M::mem_process_seqs] Processed 674918 reads in 105.138 CPU sec, 3.335 real sec
sorting block 140, #reads = 1076502 mem = 536877085 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281761, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 343, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (361.43, 164.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682126 sequences (100000088 bp) in 2.293 CPU sec, 2.337 real sec...
[M::process] read 680972 sequences (100000155 bp) in 1.510 CPU sec, 1.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282341, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 325, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (344.60, 160.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675822 reads in 177.445 CPU sec, 5.636 real sec
sorting block 141, #reads = 1074801 mem = 536879845 peak = 1230295040
[M::mem_process_seqs] Processed 678864 reads in 122.490 CPU sec, 3.901 real sec
[M::process] read 680438 sequences (100000270 bp) in 2.275 CPU sec, 2.310 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283512, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862)
[M::mem_pestat] mean and std.dev: (323.90, 150.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680132 sequences (100000208 bp) in 1.520 CPU sec, 1.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282861, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 311, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (329.32, 152.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682126 reads in 161.133 CPU sec, 5.107 real sec
sorting block 142, #reads = 1071605 mem = 536876546 peak = 1230295040
[M::mem_process_seqs] Processed 680972 reads in 103.100 CPU sec, 3.285 real sec
sorting block 143, #reads = 1080125 mem = 536884580 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283164, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 314, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (331.84, 152.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679536 sequences (100000178 bp) in 2.702 CPU sec, 2.748 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282843, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 316, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (333.60, 153.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679350 sequences (100000162 bp) in 1.900 CPU sec, 1.905 real sec...
[M::mem_process_seqs] Processed 680438 reads in 157.699 CPU sec, 4.975 real sec
[M::mem_process_seqs] Processed 680132 reads in 108.542 CPU sec, 3.430 real sec
sorting block 144, #reads = 1081166 mem = 536876648 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282757, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 319, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (335.74, 153.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678842 sequences (100000068 bp) in 2.401 CPU sec, 2.427 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283170, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (337.87, 153.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679536 reads in 151.733 CPU sec, 4.788 real sec
[M::process] read 678468 sequences (100000179 bp) in 1.980 CPU sec, 1.984 real sec...
sorting block 145, #reads = 1080050 mem = 536880165 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282459, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 325, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (340.79, 153.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678110 sequences (100000227 bp) in 2.135 CPU sec, 2.135 real sec...
[M::mem_process_seqs] Processed 678842 reads in 89.609 CPU sec, 3.051 real sec
[M::mem_process_seqs] Processed 679350 reads in 207.266 CPU sec, 6.775 real sec
sorting block 146, #reads = 1080130 mem = 536878901 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282301, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 326, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (341.76, 153.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678022 sequences (100000181 bp) in 2.308 CPU sec, 2.350 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282385, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (342.75, 152.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678468 reads in 169.564 CPU sec, 5.359 real sec
[M::process] read 677632 sequences (100000150 bp) in 2.007 CPU sec, 2.003 real sec...
[M::mem_process_seqs] Processed 678110 reads in 100.565 CPU sec, 3.155 real sec
sorting block 147, #reads = 1080400 mem = 536877516 peak = 1230295040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282401, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (345.33, 154.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677736 sequences (100000086 bp) in 2.623 CPU sec, 2.676 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282574, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (346.98, 154.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677812 sequences (100000154 bp) in 1.920 CPU sec, 1.924 real sec...
[M::mem_process_seqs] Processed 678022 reads in 160.872 CPU sec, 5.076 real sec
sorting block 148, #reads = 1079632 mem = 536877199 peak = 1230295040
[M::mem_process_seqs] Processed 677632 reads in 107.900 CPU sec, 3.405 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282380, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (347.90, 155.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677782 sequences (100000087 bp) in 2.392 CPU sec, 2.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282157, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 332, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (346.09, 154.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677582 sequences (100000296 bp) in 1.950 CPU sec, 1.953 real sec...
[M::mem_process_seqs] Processed 677736 reads in 152.786 CPU sec, 4.821 real sec
sorting block 149, #reads = 1077461 mem = 536879832 peak = 1230295040
[M::mem_process_seqs] Processed 677812 reads in 100.351 CPU sec, 3.159 real sec
sorting block 150, #reads = 1079658 mem = 536877883 peak = 1270710272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282113, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (347.59, 155.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677142 sequences (100000170 bp) in 2.477 CPU sec, 2.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281769, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (345.99, 153.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677144 sequences (100000105 bp) in 1.990 CPU sec, 1.986 real sec...
[M::mem_process_seqs] Processed 677782 reads in 154.372 CPU sec, 4.867 real sec
[M::mem_process_seqs] Processed 677582 reads in 101.917 CPU sec, 3.214 real sec
sorting block 151, #reads = 1079252 mem = 536880901 peak = 1270710272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282418, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (349.00, 153.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677540 sequences (100000241 bp) in 2.399 CPU sec, 2.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281836, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (349.36, 153.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677502 sequences (100000131 bp) in 2.040 CPU sec, 2.040 real sec...
[M::mem_process_seqs] Processed 677142 reads in 156.055 CPU sec, 4.922 real sec
[M::mem_process_seqs] Processed 677144 reads in 99.984 CPU sec, 3.147 real sec
sorting block 152, #reads = 1078097 mem = 536877705 peak = 1270710272
[M::process] read 676804 sequences (100000203 bp) in 2.033 CPU sec, 2.084 real sec...
[M::process] read 676432 sequences (100000152 bp) in 1.430 CPU sec, 1.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282088, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (345.30, 153.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677502 reads in 72.788 CPU sec, 2.299 real sec
sorting block 153, #reads = 1078435 mem = 536879947 peak = 1270710272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282588, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 329, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (343.85, 152.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282019, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 339, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (351.72, 154.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676176 sequences (100000175 bp) in 1.607 CPU sec, 1.608 real sec...
[M::mem_process_seqs] Processed 677540 reads in 199.192 CPU sec, 6.300 real sec
[M::mem_process_seqs] Processed 676804 reads in 101.186 CPU sec, 3.219 real sec
sorting block 154, #reads = 1077662 mem = 536879482 peak = 1270710272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281467, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 343, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (355.39, 155.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676258 sequences (100000149 bp) in 2.088 CPU sec, 2.153 real sec...
[M::process] read 676124 sequences (100000039 bp) in 1.520 CPU sec, 1.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282425, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 342, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (354.93, 154.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1048, 1401, 1920)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3664)
[M::mem_pestat] mean and std.dev: (1326.64, 907.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4956)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 676432 reads in 151.734 CPU sec, 4.810 real sec
sorting block 155, #reads = 1076493 mem = 536879557 peak = 1270710272
[M::mem_process_seqs] Processed 676176 reads in 100.449 CPU sec, 3.190 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281964, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 342, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (354.78, 155.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675926 sequences (100000183 bp) in 2.295 CPU sec, 2.346 real sec...
[M::process] read 676320 sequences (100000170 bp) in 1.560 CPU sec, 1.557 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281328, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 342, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (356.26, 156.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676258 reads in 160.162 CPU sec, 5.070 real sec
sorting block 156, #reads = 1074714 mem = 536879991 peak = 1270710272
[M::mem_process_seqs] Processed 676124 reads in 106.277 CPU sec, 3.376 real sec
sorting block 157, #reads = 1074370 mem = 536878739 peak = 1270710272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281739, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 342, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (357.42, 158.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676446 sequences (100000284 bp) in 2.166 CPU sec, 2.208 real sec...
[M::process] read 675908 sequences (100000015 bp) in 1.520 CPU sec, 1.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281924, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 337, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (352.66, 156.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675926 reads in 164.646 CPU sec, 5.225 real sec
[M::mem_process_seqs] Processed 676320 reads in 109.290 CPU sec, 3.478 real sec
sorting block 158, #reads = 1074873 mem = 536878491 peak = 1270710272
[M::process] read 675848 sequences (100000158 bp) in 2.176 CPU sec, 2.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281849, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 336, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (351.27, 154.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675766 sequences (100000191 bp) in 1.550 CPU sec, 1.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281890, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 340, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (354.85, 155.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676446 reads in 160.855 CPU sec, 5.094 real sec
[M::mem_process_seqs] Processed 675908 reads in 179.161 CPU sec, 5.693 real sec
sorting block 159, #reads = 1074324 mem = 536879879 peak = 1270710272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281451, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (358.66, 158.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675662 sequences (100000041 bp) in 2.373 CPU sec, 2.404 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281708, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 343, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (358.29, 158.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675602 sequences (100000040 bp) in 2.010 CPU sec, 2.014 real sec...
[M::mem_process_seqs] Processed 675848 reads in 158.871 CPU sec, 5.010 real sec
sorting block 160, #reads = 1075301 mem = 536879729 peak = 1270710272
[M::mem_process_seqs] Processed 675766 reads in 106.036 CPU sec, 3.349 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281597, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 344, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (359.04, 158.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675576 sequences (100000298 bp) in 2.629 CPU sec, 2.665 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281076, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 343, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (358.90, 159.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676026 sequences (100000262 bp) in 2.050 CPU sec, 2.054 real sec...
[M::mem_process_seqs] Processed 675662 reads in 165.146 CPU sec, 5.203 real sec
sorting block 161, #reads = 1074348 mem = 536879962 peak = 1270710272
[M::mem_process_seqs] Processed 675602 reads in 110.128 CPU sec, 3.483 real sec
sorting block 162, #reads = 1071884 mem = 536879813 peak = 1270710272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281376, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 344, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (359.82, 159.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676562 sequences (100000122 bp) in 2.428 CPU sec, 2.453 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281132, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 342, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (358.53, 160.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685942 sequences (100000230 bp) in 2.080 CPU sec, 2.081 real sec...
[M::mem_process_seqs] Processed 675576 reads in 170.545 CPU sec, 5.387 real sec
[M::mem_process_seqs] Processed 676026 reads in 116.505 CPU sec, 3.683 real sec
sorting block 163, #reads = 1071910 mem = 536879605 peak = 1270710272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285342, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (302.22, 142.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683996 sequences (100000013 bp) in 2.691 CPU sec, 2.719 real sec...
[M::process] read 683002 sequences (100000019 bp) in 2.060 CPU sec, 2.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281676, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 338, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (355.27, 160.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685942 reads in 183.290 CPU sec, 5.788 real sec
sorting block 164, #reads = 1073384 mem = 536881029 peak = 1270710272
[M::mem_process_seqs] Processed 676562 reads in 207.362 CPU sec, 6.566 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284098, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 299, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (316.96, 145.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682436 sequences (100000011 bp) in 2.731 CPU sec, 2.766 real sec...
[M::process] read 681608 sequences (100000124 bp) in 2.030 CPU sec, 2.032 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284296, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 292, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (311.97, 145.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683002 reads in 172.360 CPU sec, 5.436 real sec
sorting block 165, #reads = 1067150 mem = 536881146 peak = 1270710272
[M::mem_process_seqs] Processed 683996 reads in 192.264 CPU sec, 6.080 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284001, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 308, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (322.81, 145.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681134 sequences (100000228 bp) in 2.707 CPU sec, 2.738 real sec...
[M::process] read 680352 sequences (100000242 bp) in 2.070 CPU sec, 2.069 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284155, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 303, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (319.77, 145.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681608 reads in 171.055 CPU sec, 5.392 real sec
sorting block 166, #reads = 1075737 mem = 536879897 peak = 1270710272
[M::mem_process_seqs] Processed 682436 reads in 190.880 CPU sec, 6.036 real sec
sorting block 167, #reads = 1075419 mem = 536878946 peak = 1270710272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283246, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 311, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (324.81, 145.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679794 sequences (100000097 bp) in 2.434 CPU sec, 2.474 real sec...
[M::process] read 679524 sequences (100000227 bp) in 2.040 CPU sec, 2.048 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283260, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 315, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (329.73, 147.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681134 reads in 172.437 CPU sec, 5.447 real sec
[M::mem_process_seqs] Processed 680352 reads in 113.764 CPU sec, 3.598 real sec
sorting block 168, #reads = 1073580 mem = 536880943 peak = 1270710272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283492, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 320, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (333.97, 149.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678962 sequences (100000201 bp) in 2.651 CPU sec, 2.683 real sec...
[M::process] read 678528 sequences (100000218 bp) in 2.040 CPU sec, 2.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283692, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (334.81, 148.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679794 reads in 171.484 CPU sec, 5.409 real sec
sorting block 169, #reads = 1074238 mem = 536878834 peak = 1270710272
[M::mem_process_seqs] Processed 679524 reads in 190.582 CPU sec, 6.031 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282828, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (339.84, 151.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679052 sequences (100000146 bp) in 2.426 CPU sec, 2.450 real sec...
[M::process] read 678422 sequences (100000120 bp) in 2.030 CPU sec, 2.038 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282091, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (342.48, 152.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678962 reads in 168.690 CPU sec, 5.326 real sec
sorting block 170, #reads = 1074697 mem = 536877937 peak = 1270710272
[M::mem_process_seqs] Processed 678528 reads in 111.039 CPU sec, 3.512 real sec
sorting block 171, #reads = 1072812 mem = 536879848 peak = 1270710272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282747, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 327, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (340.86, 152.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678270 sequences (100000039 bp) in 2.099 CPU sec, 2.135 real sec...
[M::process] read 678020 sequences (100000265 bp) in 1.450 CPU sec, 1.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282810, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (342.51, 152.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679052 reads in 139.970 CPU sec, 4.429 real sec
[M::mem_process_seqs] Processed 678422 reads in 89.444 CPU sec, 2.835 real sec
sorting block 172, #reads = 1072906 mem = 536879629 peak = 1270710272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282574, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (346.62, 154.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679548 sequences (100000215 bp) in 1.912 CPU sec, 1.948 real sec...
[M::process] read 677926 sequences (100000049 bp) in 1.450 CPU sec, 1.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282225, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (346.74, 154.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678270 reads in 136.013 CPU sec, 4.311 real sec
[M::process] read 677262 sequences (100000287 bp) in 1.567 CPU sec, 1.570 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283567, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 320, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (336.38, 152.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679548 reads in 77.059 CPU sec, 2.621 real sec
sorting block 173, #reads = 1074220 mem = 536878684 peak = 1270710272
[M::mem_process_seqs] Processed 678020 reads in 182.549 CPU sec, 5.968 real sec
sorting block 174, #reads = 1075798 mem = 536880338 peak = 1334902784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282379, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 327, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (341.45, 150.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676608 sequences (100000241 bp) in 1.831 CPU sec, 1.869 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282014, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (343.42, 150.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676422 sequences (100000046 bp) in 1.420 CPU sec, 1.419 real sec...
[M::mem_process_seqs] Processed 677926 reads in 150.995 CPU sec, 4.786 real sec
[M::process] read 676288 sequences (100000185 bp) in 1.485 CPU sec, 1.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282107, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 336, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (350.15, 153.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676608 reads in 77.116 CPU sec, 2.624 real sec
sorting block 175, #reads = 1073933 mem = 536877248 peak = 1334902784
[M::mem_process_seqs] Processed 677262 reads in 186.723 CPU sec, 6.094 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281933, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 338, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (350.74, 152.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 176, #reads = 1074153 mem = 536887160 peak = 1336823808
[M::process] read 676072 sequences (100000225 bp) in 1.852 CPU sec, 1.902 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282135, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 337, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (350.49, 151.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676068 sequences (100000074 bp) in 1.410 CPU sec, 1.410 real sec...
[M::mem_process_seqs] Processed 676422 reads in 148.939 CPU sec, 4.721 real sec
[M::mem_process_seqs] Processed 676288 reads in 87.611 CPU sec, 2.762 real sec
sorting block 177, #reads = 1075387 mem = 536881886 peak = 1336823808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280810, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 335, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (348.97, 150.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676256 sequences (100000070 bp) in 2.078 CPU sec, 2.131 real sec...
[M::process] read 675984 sequences (100000004 bp) in 1.540 CPU sec, 1.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281449, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 335, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (349.20, 151.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676072 reads in 132.452 CPU sec, 4.182 real sec
[M::mem_process_seqs] Processed 676068 reads in 84.400 CPU sec, 2.664 real sec
sorting block 178, #reads = 1074448 mem = 536884519 peak = 1336823808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281432, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 333, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (347.30, 151.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675578 sequences (100000271 bp) in 1.797 CPU sec, 1.832 real sec...
[M::process] read 675574 sequences (100000060 bp) in 1.380 CPU sec, 1.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281499, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 333, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (347.48, 150.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676256 reads in 131.054 CPU sec, 4.154 real sec
sorting block 179, #reads = 1076026 mem = 536880622 peak = 1336823808
[M::mem_process_seqs] Processed 675984 reads in 83.294 CPU sec, 2.643 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281179, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 337, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (351.42, 152.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675256 sequences (100000025 bp) in 2.112 CPU sec, 2.163 real sec...
[M::process] read 675194 sequences (100000217 bp) in 1.480 CPU sec, 1.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281128, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 336, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (351.35, 154.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675578 reads in 133.182 CPU sec, 4.208 real sec
sorting block 180, #reads = 1075545 mem = 536883469 peak = 1336823808
[M::mem_process_seqs] Processed 675574 reads in 86.892 CPU sec, 2.741 real sec
sorting block 181, #reads = 1075941 mem = 536880385 peak = 1336823808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280983, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 342, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (357.60, 157.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675084 sequences (100000215 bp) in 1.868 CPU sec, 1.921 real sec...
[M::process] read 674736 sequences (100000166 bp) in 1.560 CPU sec, 1.553 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281050, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (362.09, 159.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675256 reads in 137.580 CPU sec, 4.360 real sec
[M::mem_process_seqs] Processed 675194 reads in 88.070 CPU sec, 2.786 real sec
sorting block 182, #reads = 1076732 mem = 536878827 peak = 1336823808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281345, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (363.56, 160.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674496 sequences (100000241 bp) in 1.983 CPU sec, 2.024 real sec...
[M::process] read 674556 sequences (100000141 bp) in 1.540 CPU sec, 1.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280920, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (365.15, 161.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675084 reads in 135.923 CPU sec, 4.300 real sec
[M::mem_process_seqs] Processed 674736 reads in 91.289 CPU sec, 2.894 real sec
sorting block 183, #reads = 1076965 mem = 536877580 peak = 1336823808
[M::process] read 266200 sequences (39377180 bp) in 1.268 CPU sec, 1.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280847, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 353, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (370.03, 165.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674556 reads in 72.793 CPU sec, 2.348 real sec
sorting block 184, #reads = 1077117 mem = 536877939 peak = 1336823808
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281039, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 353, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (369.84, 164.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 110877, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 343, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (358.01, 160.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674496 reads in 174.961 CPU sec, 5.644 real sec
[M::mem_process_seqs] Processed 266200 reads in 119.958 CPU sec, 3.875 real sec
sorting block 185, #reads = 1075635 mem = 536878486 peak = 1336823808
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 199859934
overall: 69984223232 mem 26449.184 user 210.766 sys 856.483 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_bR4-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:659_bR4-T1-TDNA-1_A23MHWWLT4_1\tLB:659_bR4-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_bR4-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_bR4-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 856.510 sec; CPU: 26659.953 sec
sorting block 186, #reads = 183593 mem = 92142826 peak = 1336823808
uniq header count 1
merging 187 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 96
algo: util-sort
license: sentieon:util=1
output file size: 11065277539
output reads: 200690564
bam_mem_sort: 187 calls 99.500 user 0.214 sys 100.223 real
bam_write: 187 calls 54.918 user 1.902 sys 57.154 real
execute: 1 calls 40.550 user 1.128 sys 930.971 real
merge_files: 1 calls 40.538 user 1.086 sys 74.304 real
parse_chunk: 2979 calls 171.795 user 30.594 sys 204.999 real
read_chunk: 8026 calls 63.114 user 54.146 sys 839.507 real
sort_block: 1 calls 40.654 user 53.636 sys 808.266 real
write_chunk: 5045 calls 40.146 user 0.784 sys 73.295 real
overall: 3435487232 mem 2960.033 user 106.245 sys 930.988 real
ls: cannot access '*.cram': No such file or directory