This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_cfy-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2104.186 CPU sec, 88.905 real sec
[M::process] read 688194 sequences (100000065 bp) in 1.017 CPU sec, 1.019 real sec...
[M::process] read 688184 sequences (100000176 bp) in 1.596 CPU sec, 1.644 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286881, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 264, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (286.31, 135.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688010 sequences (100000294 bp) in 1.762 CPU sec, 1.827 real sec...
[M::process] read 687886 sequences (100000227 bp) in 1.647 CPU sec, 1.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286452, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 266, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (287.22, 135.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688194 reads in 181.449 CPU sec, 6.131 real sec
[M::mem_process_seqs] Processed 688184 reads in 104.805 CPU sec, 3.705 real sec
sorting block 0, #reads = 1088621 mem = 536880180 peak = 575737856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286909, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 266, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (287.17, 135.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687596 sequences (100000215 bp) in 2.165 CPU sec, 2.380 real sec...
[M::process] read 687632 sequences (100000229 bp) in 1.727 CPU sec, 1.743 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286538, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 267, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (288.47, 136.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688010 reads in 166.013 CPU sec, 5.420 real sec
[M::mem_process_seqs] Processed 687886 reads in 106.733 CPU sec, 3.527 real sec
sorting block 1, #reads = 1089856 mem = 536876970 peak = 699994112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287151, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 268, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (290.19, 137.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687034 sequences (100000254 bp) in 1.936 CPU sec, 2.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286132, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (291.30, 138.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687046 sequences (100000069 bp) in 1.757 CPU sec, 1.787 real sec...
[M::mem_process_seqs] Processed 687596 reads in 167.750 CPU sec, 5.565 real sec
[M::mem_process_seqs] Processed 687632 reads in 106.507 CPU sec, 3.535 real sec
sorting block 2, #reads = 1088886 mem = 536881587 peak = 809627648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287086, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 271, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (293.54, 139.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688868 sequences (100000034 bp) in 2.282 CPU sec, 2.665 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286785, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (294.67, 140.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687034 reads in 167.469 CPU sec, 5.555 real sec
[M::process] read 686196 sequences (100000017 bp) in 1.960 CPU sec, 2.050 real sec...
[M::mem_process_seqs] Processed 687046 reads in 110.035 CPU sec, 3.676 real sec
sorting block 3, #reads = 1088457 mem = 536881053 peak = 809627648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287902, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 262, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761)
[M::mem_pestat] mean and std.dev: (285.44, 136.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1088660 mem = 536877039 peak = 869777408
[M::process] read 685872 sequences (100000105 bp) in 2.244 CPU sec, 2.810 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285882, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 276, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (298.32, 141.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688868 reads in 168.970 CPU sec, 5.657 real sec
[M::process] read 685210 sequences (100000039 bp) in 1.846 CPU sec, 2.056 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285949, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (300.65, 142.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685872 reads in 92.363 CPU sec, 3.021 real sec
[M::process] read 685044 sequences (100000269 bp) in 2.170 CPU sec, 2.310 real sec...
sorting block 5, #reads = 1088895 mem = 536879740 peak = 869777408
[M::mem_process_seqs] Processed 686196 reads in 256.436 CPU sec, 8.487 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285821, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (302.23, 143.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684812 sequences (100000257 bp) in 2.277 CPU sec, 2.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286146, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 283, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (304.33, 144.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685210 reads in 182.506 CPU sec, 6.033 real sec
sorting block 6, #reads = 1088096 mem = 536878553 peak = 869777408
[M::mem_process_seqs] Processed 685044 reads in 107.546 CPU sec, 3.639 real sec
[M::process] read 684752 sequences (100000110 bp) in 2.268 CPU sec, 2.689 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285640, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 283, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (304.19, 143.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 7, #reads = 1087355 mem = 536878834 peak = 869777408
[M::process] read 684800 sequences (100000232 bp) in 2.353 CPU sec, 2.601 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285425, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 283, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (303.33, 142.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684812 reads in 168.022 CPU sec, 5.735 real sec
[M::mem_process_seqs] Processed 684752 reads in 106.869 CPU sec, 3.661 real sec
[M::process] read 685446 sequences (100000126 bp) in 2.768 CPU sec, 3.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285918, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (304.79, 143.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 8, #reads = 1086743 mem = 536879609 peak = 869777408
[M::process] read 684926 sequences (100000288 bp) in 2.332 CPU sec, 2.593 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285695, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (299.45, 141.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684800 reads in 170.403 CPU sec, 5.586 real sec
[M::mem_process_seqs] Processed 685446 reads in 108.179 CPU sec, 3.603 real sec
[M::process] read 683906 sequences (100000202 bp) in 2.338 CPU sec, 2.767 real sec...
sorting block 9, #reads = 1087250 mem = 536878409 peak = 869777408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286328, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (300.12, 141.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285890, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (304.87, 141.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (117, 214, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146)
[M::mem_pestat] mean and std.dev: (250.50, 142.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1489)
[M::mem_pestat] skip orientation RR
[M::process] read 683718 sequences (100000085 bp) in 2.736 CPU sec, 3.057 real sec...
[M::mem_process_seqs] Processed 684926 reads in 174.371 CPU sec, 5.899 real sec
[M::mem_process_seqs] Processed 683906 reads in 112.440 CPU sec, 3.819 real sec
sorting block 10, #reads = 1088067 mem = 536879147 peak = 869777408
[M::process] read 684150 sequences (100000202 bp) in 2.501 CPU sec, 2.872 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285187, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 286, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (305.44, 142.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683718 reads in 92.473 CPU sec, 3.289 real sec
[M::process] read 683436 sequences (100000118 bp) in 2.213 CPU sec, 2.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285573, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (306.12, 143.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 11, #reads = 1087562 mem = 536885702 peak = 869777408
[M::mem_process_seqs] Processed 684150 reads in 95.128 CPU sec, 3.396 real sec
[M::process] read 683266 sequences (100000285 bp) in 2.339 CPU sec, 2.683 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285191, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 288, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (307.20, 142.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683436 reads in 92.014 CPU sec, 3.240 real sec
sorting block 12, #reads = 1086672 mem = 536880162 peak = 869777408
[M::process] read 683140 sequences (100000252 bp) in 2.389 CPU sec, 2.732 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285397, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 287, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (305.60, 140.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683266 reads in 94.098 CPU sec, 3.378 real sec
[M::process] read 683546 sequences (100000085 bp) in 2.135 CPU sec, 2.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285175, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 287, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (306.25, 141.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683140 reads in 93.110 CPU sec, 3.321 real sec
sorting block 13, #reads = 1085964 mem = 536879982 peak = 869777408
[M::process] read 683760 sequences (100000102 bp) in 2.280 CPU sec, 2.513 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285204, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (304.63, 141.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683546 reads in 98.604 CPU sec, 3.455 real sec
sorting block 14, #reads = 1087226 mem = 536880964 peak = 869777408
[M::process] read 683548 sequences (100000253 bp) in 2.678 CPU sec, 3.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285393, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (304.21, 141.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683760 reads in 92.912 CPU sec, 3.341 real sec
[M::process] read 683916 sequences (100000177 bp) in 2.558 CPU sec, 2.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285260, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 284, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (303.43, 140.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 15, #reads = 1086268 mem = 536876704 peak = 869777408
[M::process] read 683628 sequences (100000103 bp) in 2.234 CPU sec, 2.509 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285382, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 283, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (302.35, 140.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683548 reads in 171.697 CPU sec, 5.954 real sec
[M::mem_process_seqs] Processed 683916 reads in 105.160 CPU sec, 3.731 real sec
[M::process] read 682904 sequences (100000150 bp) in 2.372 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285198, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (303.31, 140.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1086988 mem = 536879325 peak = 869777408
[M::mem_process_seqs] Processed 683628 reads in 95.020 CPU sec, 3.343 real sec
[M::process] read 682862 sequences (100000180 bp) in 2.328 CPU sec, 2.741 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285191, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 288, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (307.28, 142.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682904 reads in 92.700 CPU sec, 3.411 real sec
sorting block 17, #reads = 1086905 mem = 536878839 peak = 869777408
[M::process] read 682884 sequences (100000055 bp) in 2.319 CPU sec, 2.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284902, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 288, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (306.02, 140.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682862 reads in 92.932 CPU sec, 3.480 real sec
[M::process] read 682772 sequences (100000105 bp) in 2.342 CPU sec, 2.707 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284869, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 288, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (306.68, 141.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 18, #reads = 1086361 mem = 536875464 peak = 869777408
[M::mem_process_seqs] Processed 682884 reads in 92.655 CPU sec, 3.440 real sec
[M::process] read 682914 sequences (100000086 bp) in 2.507 CPU sec, 2.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285320, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 289, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (308.05, 141.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682772 reads in 96.097 CPU sec, 3.524 real sec
sorting block 19, #reads = 1086382 mem = 536878555 peak = 869777408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285015, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 290, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (309.38, 143.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688202 sequences (100000014 bp) in 2.586 CPU sec, 3.106 real sec...
[M::mem_process_seqs] Processed 682914 reads in 93.508 CPU sec, 3.325 real sec
[M::process] read 688226 sequences (100000069 bp) in 2.606 CPU sec, 2.915 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286866, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 267, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (289.31, 138.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688202 reads in 97.711 CPU sec, 3.447 real sec
sorting block 20, #reads = 1086216 mem = 536880312 peak = 869777408
[M::process] read 687836 sequences (100000098 bp) in 2.281 CPU sec, 2.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286919, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (289.38, 138.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688226 reads in 92.109 CPU sec, 3.343 real sec
[M::process] read 687634 sequences (100000036 bp) in 2.350 CPU sec, 2.914 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286830, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 268, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (290.65, 138.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1086698 mem = 536879659 peak = 871501824
[M::mem_process_seqs] Processed 687836 reads in 94.181 CPU sec, 3.339 real sec
[M::process] read 687650 sequences (100000296 bp) in 2.308 CPU sec, 2.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286598, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (292.40, 139.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687634 reads in 95.095 CPU sec, 3.394 real sec
sorting block 22, #reads = 1090345 mem = 536876675 peak = 871501824
[M::process] read 687320 sequences (100000042 bp) in 2.420 CPU sec, 2.862 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286567, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (291.91, 138.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687650 reads in 96.441 CPU sec, 3.553 real sec
[M::process] read 686906 sequences (100000061 bp) in 2.425 CPU sec, 2.866 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285848, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 271, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (293.26, 139.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 23, #reads = 1089341 mem = 536880643 peak = 871501824
[M::mem_process_seqs] Processed 687320 reads in 95.977 CPU sec, 3.405 real sec
[M::process] read 687018 sequences (100000132 bp) in 2.399 CPU sec, 2.683 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285851, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 273, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (295.03, 140.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686906 reads in 94.608 CPU sec, 3.300 real sec
sorting block 24, #reads = 1089677 mem = 536878895 peak = 877117440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286236, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 275, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (296.19, 140.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686752 sequences (100000191 bp) in 2.665 CPU sec, 2.978 real sec...
[M::mem_process_seqs] Processed 687018 reads in 92.036 CPU sec, 3.130 real sec
[M::process] read 686366 sequences (100000032 bp) in 2.427 CPU sec, 2.795 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286405, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 275, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (297.48, 141.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1089197 mem = 536877959 peak = 877117440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286683, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 278, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (300.59, 144.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685850 sequences (100000037 bp) in 2.474 CPU sec, 2.767 real sec...
[M::mem_process_seqs] Processed 686752 reads in 164.723 CPU sec, 5.424 real sec
sorting block 26, #reads = 1089192 mem = 536878307 peak = 877117440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286178, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 281, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (303.05, 145.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685448 sequences (100000075 bp) in 2.475 CPU sec, 2.789 real sec...
[M::mem_process_seqs] Processed 685850 reads in 87.567 CPU sec, 2.896 real sec
[M::process] read 684992 sequences (100000234 bp) in 2.145 CPU sec, 2.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285588, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 283, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (304.73, 145.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686366 reads in 280.156 CPU sec, 9.271 real sec
[M::mem_process_seqs] Processed 685448 reads in 88.043 CPU sec, 2.942 real sec
sorting block 27, #reads = 1089526 mem = 536878783 peak = 877117440
[M::process] read 684868 sequences (100000012 bp) in 2.304 CPU sec, 2.564 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286211, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (306.18, 145.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286328, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 286, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (307.38, 146.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684754 sequences (100000291 bp) in 2.281 CPU sec, 2.597 real sec...
[M::mem_process_seqs] Processed 684992 reads in 163.116 CPU sec, 5.412 real sec
sorting block 28, #reads = 1088803 mem = 536879146 peak = 879759360
[M::mem_process_seqs] Processed 684868 reads in 104.248 CPU sec, 3.491 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285536, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 285, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (305.71, 144.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684294 sequences (100000245 bp) in 2.480 CPU sec, 2.934 real sec...
[M::mem_process_seqs] Processed 684754 reads in 90.967 CPU sec, 3.198 real sec
sorting block 29, #reads = 1088134 mem = 536883214 peak = 890298368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285723, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 287, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (307.22, 144.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684396 sequences (100000098 bp) in 2.550 CPU sec, 2.766 real sec...
[M::mem_process_seqs] Processed 684294 reads in 91.163 CPU sec, 3.261 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285699, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 288, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (308.20, 144.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684438 sequences (100000181 bp) in 2.305 CPU sec, 2.655 real sec...
sorting block 30, #reads = 1087515 mem = 536877242 peak = 890298368
[M::mem_process_seqs] Processed 684396 reads in 88.051 CPU sec, 3.202 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285767, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (304.71, 142.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684028 sequences (100000178 bp) in 2.233 CPU sec, 2.532 real sec...
[M::mem_process_seqs] Processed 684438 reads in 90.134 CPU sec, 3.120 real sec
sorting block 31, #reads = 1086853 mem = 536879988 peak = 890298368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285535, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 287, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (306.74, 143.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (86, 86, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (155.20, 127.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 683382 sequences (100000102 bp) in 2.235 CPU sec, 2.689 real sec...
[M::mem_process_seqs] Processed 684028 reads in 88.926 CPU sec, 3.239 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285239, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 288, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (307.69, 142.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683572 sequences (100000057 bp) in 2.544 CPU sec, 2.957 real sec...
[M::mem_process_seqs] Processed 683382 reads in 93.603 CPU sec, 3.237 real sec
sorting block 32, #reads = 1087031 mem = 536876598 peak = 890298368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285198, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 288, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (306.92, 142.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683416 sequences (100000067 bp) in 2.383 CPU sec, 2.684 real sec...
[M::mem_process_seqs] Processed 683572 reads in 90.081 CPU sec, 3.157 real sec
sorting block 33, #reads = 1085944 mem = 536879732 peak = 903278592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285789, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 288, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (307.20, 142.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683466 sequences (100000264 bp) in 2.619 CPU sec, 2.926 real sec...
[M::mem_process_seqs] Processed 683416 reads in 94.100 CPU sec, 3.245 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285585, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 289, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (308.91, 144.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683088 sequences (100000104 bp) in 2.256 CPU sec, 2.724 real sec...
[M::mem_process_seqs] Processed 683466 reads in 88.990 CPU sec, 3.063 real sec
sorting block 34, #reads = 1087224 mem = 536878422 peak = 903278592
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284949, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 288, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (306.50, 141.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683348 sequences (100000089 bp) in 2.373 CPU sec, 2.710 real sec...
[M::mem_process_seqs] Processed 683088 reads in 94.068 CPU sec, 3.190 real sec
[M::process] read 683472 sequences (100000195 bp) in 2.190 CPU sec, 2.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285089, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 288, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (305.72, 140.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 35, #reads = 1086144 mem = 536889121 peak = 908296192
[M::mem_process_seqs] Processed 683348 reads in 89.438 CPU sec, 3.103 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285495, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (304.29, 140.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683708 sequences (100000240 bp) in 2.511 CPU sec, 2.853 real sec...
[M::mem_process_seqs] Processed 683472 reads in 92.832 CPU sec, 3.150 real sec
sorting block 36, #reads = 1085117 mem = 536885750 peak = 908296192
[M::process] read 683664 sequences (100000263 bp) in 2.349 CPU sec, 2.803 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285492, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 284, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (303.58, 140.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683708 reads in 91.201 CPU sec, 3.173 real sec
[M::process] read 683642 sequences (100000114 bp) in 2.480 CPU sec, 2.808 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285751, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (304.32, 141.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 37, #reads = 1086511 mem = 536878922 peak = 908296192
[M::mem_process_seqs] Processed 683664 reads in 92.898 CPU sec, 3.223 real sec
[M::process] read 683898 sequences (100000044 bp) in 2.192 CPU sec, 2.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285485, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (304.18, 140.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1085895 mem = 536878893 peak = 908296192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285567, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 284, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (303.29, 139.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683604 sequences (100000104 bp) in 2.437 CPU sec, 2.778 real sec...
[M::mem_process_seqs] Processed 683642 reads in 166.804 CPU sec, 5.581 real sec
[M::mem_process_seqs] Processed 683898 reads in 106.992 CPU sec, 3.659 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285185, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 284, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (303.98, 140.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683698 sequences (100000127 bp) in 2.514 CPU sec, 2.959 real sec...
[M::mem_process_seqs] Processed 683604 reads in 92.288 CPU sec, 3.211 real sec
sorting block 39, #reads = 1086268 mem = 536879499 peak = 908296192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285250, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 284, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (303.25, 140.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683836 sequences (100000084 bp) in 2.557 CPU sec, 2.790 real sec...
[M::mem_process_seqs] Processed 683698 reads in 89.705 CPU sec, 3.142 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285641, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (304.18, 141.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (1868, 1868, 1868)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1868, 1868)
[M::mem_pestat] mean and std.dev: (1868.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1868, 1868)
[M::mem_pestat] skip orientation RR
[M::process] read 683218 sequences (100000146 bp) in 2.313 CPU sec, 2.735 real sec...
sorting block 40, #reads = 1086188 mem = 536879419 peak = 908296192
[M::mem_process_seqs] Processed 683836 reads in 91.395 CPU sec, 3.225 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285673, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 288, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (307.77, 143.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686416 sequences (100000112 bp) in 2.282 CPU sec, 2.644 real sec...
[M::mem_process_seqs] Processed 683218 reads in 91.480 CPU sec, 3.196 real sec
sorting block 41, #reads = 1085775 mem = 536881888 peak = 908296192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286323, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 274, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (296.18, 140.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688708 sequences (100000076 bp) in 2.363 CPU sec, 2.625 real sec...
[M::mem_process_seqs] Processed 686416 reads in 93.043 CPU sec, 3.378 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287129, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 264, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (287.14, 137.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688456 sequences (100000035 bp) in 2.578 CPU sec, 2.956 real sec...
sorting block 42, #reads = 1085615 mem = 536879608 peak = 908296192
[M::mem_process_seqs] Processed 688708 reads in 93.837 CPU sec, 3.232 real sec
[M::process] read 687960 sequences (100000241 bp) in 2.145 CPU sec, 2.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286835, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 265, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (288.41, 137.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688456 reads in 91.185 CPU sec, 3.184 real sec
sorting block 43, #reads = 1087314 mem = 536878590 peak = 908296192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287077, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 191, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (119, 299)
[M::mem_pestat] mean and std.dev: (195.00, 11.31)
[M::mem_pestat] low and high boundaries for proper pairs: (83, 335)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 268, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (290.34, 138.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687500 sequences (100000252 bp) in 2.537 CPU sec, 2.861 real sec...
[M::mem_process_seqs] Processed 687960 reads in 92.681 CPU sec, 3.228 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287083, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (291.59, 138.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687184 sequences (100000191 bp) in 2.448 CPU sec, 2.729 real sec...
[M::mem_process_seqs] Processed 687500 reads in 92.404 CPU sec, 3.233 real sec
sorting block 44, #reads = 1087972 mem = 536879290 peak = 908296192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286970, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 271, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (292.62, 138.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687484 sequences (100000291 bp) in 2.200 CPU sec, 2.690 real sec...
[M::mem_process_seqs] Processed 687184 reads in 90.862 CPU sec, 3.181 real sec
sorting block 45, #reads = 1089366 mem = 536884297 peak = 908296192
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286662, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 271, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (292.66, 138.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687212 sequences (100000192 bp) in 2.329 CPU sec, 2.754 real sec...
[M::mem_process_seqs] Processed 687484 reads in 92.249 CPU sec, 3.210 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286523, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 686092 sequences (100000070 bp) in 2.284 CPU sec, 2.570 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (185, 273, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (295.33, 140.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687212 reads in 89.989 CPU sec, 3.198 real sec
sorting block 46, #reads = 1088937 mem = 536876890 peak = 908738560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286191, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (298.22, 140.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686176 sequences (100000091 bp) in 2.309 CPU sec, 2.758 real sec...
[M::mem_process_seqs] Processed 686092 reads in 92.685 CPU sec, 3.260 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286592, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 277, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (299.08, 141.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685864 sequences (100000200 bp) in 2.320 CPU sec, 2.643 real sec...
sorting block 47, #reads = 1087734 mem = 536881589 peak = 916897792
[M::mem_process_seqs] Processed 686176 reads in 89.020 CPU sec, 3.065 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286683, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (300.40, 142.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685676 sequences (100000017 bp) in 2.486 CPU sec, 3.071 real sec...
[M::mem_process_seqs] Processed 685864 reads in 93.590 CPU sec, 3.274 real sec
sorting block 48, #reads = 1087518 mem = 536883963 peak = 916897792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286449, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 281, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (303.34, 144.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685404 sequences (100000019 bp) in 2.468 CPU sec, 2.753 real sec...
[M::mem_process_seqs] Processed 685676 reads in 88.154 CPU sec, 3.183 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286241, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (304.43, 145.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684888 sequences (100000091 bp) in 2.434 CPU sec, 2.971 real sec...
[M::mem_process_seqs] Processed 685404 reads in 95.003 CPU sec, 3.312 real sec
sorting block 49, #reads = 1087593 mem = 536878738 peak = 916897792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285639, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (305.72, 145.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684822 sequences (100000189 bp) in 2.263 CPU sec, 2.896 real sec...
[M::mem_process_seqs] Processed 684888 reads in 87.551 CPU sec, 3.093 real sec
sorting block 50, #reads = 1088422 mem = 536878194 peak = 916897792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286412, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (305.64, 145.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684936 sequences (100000207 bp) in 2.580 CPU sec, 2.869 real sec...
[M::mem_process_seqs] Processed 684822 reads in 93.391 CPU sec, 3.294 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285934, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (305.54, 144.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684744 sequences (100000232 bp) in 2.316 CPU sec, 2.834 real sec...
[M::mem_process_seqs] Processed 684936 reads in 89.483 CPU sec, 3.174 real sec
sorting block 51, #reads = 1088067 mem = 536881764 peak = 916897792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286092, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (305.74, 145.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684172 sequences (100000216 bp) in 2.219 CPU sec, 2.778 real sec...
[M::mem_process_seqs] Processed 684744 reads in 90.593 CPU sec, 3.142 real sec
sorting block 52, #reads = 1086580 mem = 536877655 peak = 916897792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285877, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (306.85, 144.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684730 sequences (100000295 bp) in 2.411 CPU sec, 2.707 real sec...
[M::mem_process_seqs] Processed 684172 reads in 90.131 CPU sec, 3.146 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285674, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (304.02, 144.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684638 sequences (100000145 bp) in 2.359 CPU sec, 2.957 real sec...
[M::mem_process_seqs] Processed 684730 reads in 90.725 CPU sec, 3.207 real sec
sorting block 53, #reads = 1087908 mem = 536879273 peak = 930181120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285126, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (303.85, 143.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684690 sequences (100000271 bp) in 2.238 CPU sec, 2.557 real sec...
[M::mem_process_seqs] Processed 684638 reads in 90.313 CPU sec, 3.196 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286238, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (304.49, 144.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684888 sequences (100000016 bp) in 2.394 CPU sec, 2.806 real sec...
sorting block 54, #reads = 1086019 mem = 536877678 peak = 940265472
[M::mem_process_seqs] Processed 684690 reads in 92.649 CPU sec, 3.246 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286204, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (303.06, 143.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684912 sequences (100000058 bp) in 2.238 CPU sec, 2.617 real sec...
[M::mem_process_seqs] Processed 684888 reads in 88.836 CPU sec, 3.144 real sec
sorting block 55, #reads = 1086732 mem = 536880024 peak = 940265472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285611, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (300.46, 141.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684366 sequences (100000298 bp) in 2.403 CPU sec, 2.853 real sec...
[M::mem_process_seqs] Processed 684912 reads in 93.053 CPU sec, 3.316 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285598, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 279, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (300.41, 140.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684576 sequences (100000081 bp) in 2.290 CPU sec, 2.572 real sec...
[M::mem_process_seqs] Processed 684366 reads in 88.944 CPU sec, 3.252 real sec
sorting block 56, #reads = 1087843 mem = 536879615 peak = 940265472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286191, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 684414 sequences (100000181 bp) in 2.365 CPU sec, 2.870 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (191, 280, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (301.23, 141.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684576 reads in 94.742 CPU sec, 3.289 real sec
sorting block 57, #reads = 1086499 mem = 536879713 peak = 940265472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285305, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 281, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (302.05, 141.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684474 sequences (100000063 bp) in 2.314 CPU sec, 2.814 real sec...
[M::mem_process_seqs] Processed 684414 reads in 90.477 CPU sec, 3.159 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285331, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (301.82, 140.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683998 sequences (100000048 bp) in 2.362 CPU sec, 2.740 real sec...
[M::mem_process_seqs] Processed 684474 reads in 93.879 CPU sec, 3.271 real sec
sorting block 58, #reads = 1085907 mem = 536880186 peak = 951357440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285453, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 283, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (302.88, 141.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684042 sequences (100000054 bp) in 2.625 CPU sec, 3.093 real sec...
[M::mem_process_seqs] Processed 683998 reads in 92.666 CPU sec, 3.283 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285569, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (304.20, 142.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684138 sequences (100000185 bp) in 2.561 CPU sec, 2.923 real sec...
sorting block 59, #reads = 1085829 mem = 536879121 peak = 951357440
[M::mem_process_seqs] Processed 684042 reads in 90.757 CPU sec, 3.109 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285691, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (304.33, 142.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683636 sequences (100000245 bp) in 2.352 CPU sec, 2.841 real sec...
[M::mem_process_seqs] Processed 684138 reads in 90.917 CPU sec, 3.150 real sec
sorting block 60, #reads = 1085039 mem = 536878011 peak = 951357440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285221, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (305.13, 142.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683682 sequences (100000168 bp) in 2.345 CPU sec, 2.719 real sec...
[M::mem_process_seqs] Processed 683636 reads in 88.079 CPU sec, 3.053 real sec
[M::process] read 683488 sequences (100000227 bp) in 2.160 CPU sec, 2.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285364, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (305.28, 142.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 61, #reads = 1086267 mem = 536881773 peak = 951357440
[M::mem_process_seqs] Processed 683682 reads in 92.717 CPU sec, 3.245 real sec
[M::process] read 684020 sequences (100000203 bp) in 2.218 CPU sec, 2.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285266, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (303.71, 140.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683488 reads in 87.054 CPU sec, 3.073 real sec
sorting block 62, #reads = 1086549 mem = 536879536 peak = 958836736
[M::process] read 687514 sequences (100000057 bp) in 2.511 CPU sec, 2.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285793, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (304.67, 141.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684020 reads in 94.233 CPU sec, 3.252 real sec
[M::process] read 688328 sequences (100000193 bp) in 2.240 CPU sec, 2.570 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286532, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (291.70, 139.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687514 reads in 94.526 CPU sec, 3.303 real sec
sorting block 63, #reads = 1085971 mem = 536878859 peak = 958836736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287211, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (290.86, 140.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687936 sequences (100000033 bp) in 2.392 CPU sec, 2.582 real sec...
[M::mem_process_seqs] Processed 688328 reads in 90.300 CPU sec, 3.159 real sec
sorting block 64, #reads = 1085496 mem = 536877423 peak = 958836736
[M::process] read 687488 sequences (100000229 bp) in 2.172 CPU sec, 2.499 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286612, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (292.74, 141.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687936 reads in 89.634 CPU sec, 3.124 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287185, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (294.17, 141.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686996 sequences (100000155 bp) in 2.480 CPU sec, 2.843 real sec...
[M::mem_process_seqs] Processed 687488 reads in 89.940 CPU sec, 3.192 real sec
sorting block 65, #reads = 1091108 mem = 536879075 peak = 958836736
[M::process] read 686946 sequences (100000027 bp) in 2.219 CPU sec, 2.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286528, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 272, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (294.99, 140.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686996 reads in 87.940 CPU sec, 3.129 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286497, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (296.22, 141.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687054 sequences (100000086 bp) in 2.362 CPU sec, 2.885 real sec...
sorting block 66, #reads = 1089903 mem = 536880261 peak = 958836736
[M::mem_process_seqs] Processed 686946 reads in 90.076 CPU sec, 3.180 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286669, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (296.71, 142.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686238 sequences (100000209 bp) in 2.340 CPU sec, 2.645 real sec...
[M::mem_process_seqs] Processed 687054 reads in 90.863 CPU sec, 3.111 real sec
sorting block 67, #reads = 1089108 mem = 536877604 peak = 958836736
[M::process] read 686312 sequences (100000038 bp) in 2.345 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286395, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 276, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (298.64, 142.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686238 reads in 89.164 CPU sec, 3.045 real sec
sorting block 68, #reads = 1089717 mem = 536879367 peak = 958836736
[M::process] read 685840 sequences (100000093 bp) in 2.391 CPU sec, 2.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286378, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 277, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (298.58, 142.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286446, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (301.06, 143.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685516 sequences (100000216 bp) in 2.418 CPU sec, 2.642 real sec...
[M::mem_process_seqs] Processed 686312 reads in 162.871 CPU sec, 5.293 real sec
[M::mem_process_seqs] Processed 685840 reads in 105.589 CPU sec, 3.406 real sec
sorting block 69, #reads = 1088574 mem = 536877480 peak = 958836736
[M::process] read 685474 sequences (100000229 bp) in 2.398 CPU sec, 2.665 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286307, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (302.14, 143.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285898, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (303.26, 144.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685362 sequences (100000150 bp) in 2.365 CPU sec, 2.580 real sec...
[M::mem_process_seqs] Processed 685516 reads in 160.891 CPU sec, 5.231 real sec
[M::mem_process_seqs] Processed 685474 reads in 104.618 CPU sec, 3.381 real sec
sorting block 70, #reads = 1089031 mem = 536879815 peak = 958836736
[M::process] read 685168 sequences (100000165 bp) in 2.465 CPU sec, 2.736 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286335, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (303.78, 144.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685362 reads in 89.455 CPU sec, 3.004 real sec
sorting block 71, #reads = 1088768 mem = 536886326 peak = 958836736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286267, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (304.86, 145.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684886 sequences (100000102 bp) in 2.534 CPU sec, 2.831 real sec...
[M::mem_process_seqs] Processed 685168 reads in 89.458 CPU sec, 3.043 real sec
[M::process] read 684974 sequences (100000121 bp) in 2.727 CPU sec, 2.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286038, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 283, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (306.08, 146.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 72, #reads = 1088128 mem = 536878800 peak = 958836736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286226, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (305.82, 146.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685008 sequences (100000150 bp) in 2.345 CPU sec, 2.652 real sec...
[M::mem_process_seqs] Processed 684886 reads in 162.342 CPU sec, 5.304 real sec
[M::mem_process_seqs] Processed 684974 reads in 104.662 CPU sec, 3.424 real sec
sorting block 73, #reads = 1088065 mem = 536881666 peak = 958836736
[M::process] read 684892 sequences (100000136 bp) in 2.254 CPU sec, 2.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286073, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (303.84, 145.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685286 sequences (100000026 bp) in 2.402 CPU sec, 2.828 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285778, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (303.98, 144.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685008 reads in 166.631 CPU sec, 5.421 real sec
sorting block 74, #reads = 1087196 mem = 536878977 peak = 958836736
[M::process] read 685386 sequences (100000205 bp) in 1.985 CPU sec, 2.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286476, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (302.15, 144.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685286 reads in 93.319 CPU sec, 3.037 real sec
[M::process] read 685378 sequences (100000197 bp) in 2.218 CPU sec, 2.393 real sec...
[M::mem_process_seqs] Processed 684892 reads in 265.270 CPU sec, 8.578 real sec
sorting block 75, #reads = 1088073 mem = 536882154 peak = 958836736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286038, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 277, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (300.42, 143.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685548 sequences (100000221 bp) in 2.057 CPU sec, 2.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285436, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (300.23, 143.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685386 reads in 179.528 CPU sec, 5.831 real sec
[M::mem_process_seqs] Processed 685378 reads in 106.495 CPU sec, 3.479 real sec
sorting block 76, #reads = 1087805 mem = 536879285 peak = 958836736
[M::process] read 685580 sequences (100000015 bp) in 2.571 CPU sec, 2.747 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285755, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 275, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (298.76, 142.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685430 sequences (100000089 bp) in 2.347 CPU sec, 2.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286177, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (299.38, 142.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685548 reads in 163.109 CPU sec, 5.312 real sec
[M::mem_process_seqs] Processed 685580 reads in 104.360 CPU sec, 3.389 real sec
sorting block 77, #reads = 1088152 mem = 536879473 peak = 958836736
[M::process] read 685314 sequences (100000220 bp) in 2.301 CPU sec, 2.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286239, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (298.07, 141.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 78, #reads = 1088218 mem = 536879801 peak = 976580608
[M::process] read 685040 sequences (100000141 bp) in 1.957 CPU sec, 2.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286349, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 276, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (298.22, 141.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685430 reads in 164.682 CPU sec, 5.328 real sec
[M::mem_process_seqs] Processed 685314 reads in 107.674 CPU sec, 3.432 real sec
[M::process] read 685014 sequences (100000292 bp) in 2.049 CPU sec, 2.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285682, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 276, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (297.65, 140.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 79, #reads = 1087538 mem = 536878996 peak = 976580608
[M::process] read 684808 sequences (100000009 bp) in 2.298 CPU sec, 2.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286451, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 277, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (298.28, 140.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685040 reads in 163.879 CPU sec, 5.308 real sec
[M::mem_process_seqs] Processed 685014 reads in 108.667 CPU sec, 3.489 real sec
[M::process] read 684460 sequences (100000145 bp) in 2.172 CPU sec, 2.275 real sec...
sorting block 80, #reads = 1086764 mem = 536879891 peak = 976580608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285491, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (300.51, 141.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684692 sequences (100000057 bp) in 2.385 CPU sec, 2.801 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285452, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 280, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (300.93, 141.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684808 reads in 159.239 CPU sec, 5.165 real sec
[M::mem_process_seqs] Processed 684460 reads in 104.822 CPU sec, 3.354 real sec
sorting block 81, #reads = 1087218 mem = 536880134 peak = 976580608
[M::process] read 684246 sequences (100000028 bp) in 1.883 CPU sec, 1.963 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286147, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 280, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (300.99, 141.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684226 sequences (100000273 bp) in 2.395 CPU sec, 2.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285669, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (303.28, 142.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684692 reads in 161.682 CPU sec, 5.220 real sec
sorting block 82, #reads = 1086677 mem = 536879096 peak = 1033187328
[M::mem_process_seqs] Processed 684246 reads in 105.306 CPU sec, 3.372 real sec
[M::process] read 684114 sequences (100000001 bp) in 1.936 CPU sec, 2.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285672, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 281, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (302.38, 141.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 83, #reads = 1087909 mem = 536878007 peak = 1043537920
[M::process] read 684076 sequences (100000301 bp) in 2.172 CPU sec, 2.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285870, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (302.60, 141.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684226 reads in 164.806 CPU sec, 5.345 real sec
[M::mem_process_seqs] Processed 684114 reads in 105.353 CPU sec, 3.392 real sec
[M::process] read 688932 sequences (100000206 bp) in 2.134 CPU sec, 2.247 real sec...
sorting block 84, #reads = 1086378 mem = 536880433 peak = 1043537920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285359, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 283, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (303.82, 142.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691734 sequences (100000083 bp) in 2.515 CPU sec, 2.803 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287373, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 262, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (287.33, 139.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684076 reads in 173.809 CPU sec, 5.626 real sec
[M::mem_process_seqs] Processed 688932 reads in 120.107 CPU sec, 3.852 real sec
[M::process] read 691822 sequences (100000300 bp) in 2.219 CPU sec, 2.293 real sec...
sorting block 85, #reads = 1086677 mem = 536882371 peak = 1043537920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288250, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 250, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.25, 137.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690730 sequences (100000179 bp) in 2.377 CPU sec, 2.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288073, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 249, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (276.51, 136.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (397, 397, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (257, 607)
[M::mem_pestat] mean and std.dev: (405.88, 64.61)
[M::mem_pestat] low and high boundaries for proper pairs: (147, 677)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 691734 reads in 178.003 CPU sec, 5.764 real sec
[M::process] read 690012 sequences (100000107 bp) in 1.847 CPU sec, 1.906 real sec...
[M::mem_process_seqs] Processed 691822 reads in 117.410 CPU sec, 3.782 real sec
sorting block 86, #reads = 1085457 mem = 536880364 peak = 1043537920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288577, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 255, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (282.41, 139.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 87, #reads = 1088571 mem = 536878720 peak = 1047580672
[M::process] read 689620 sequences (100000060 bp) in 2.876 CPU sec, 3.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288031, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 258, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (285.87, 141.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690730 reads in 171.367 CPU sec, 5.545 real sec
[M::process] read 689686 sequences (100000185 bp) in 2.056 CPU sec, 2.161 real sec...
[M::mem_process_seqs] Processed 690012 reads in 111.320 CPU sec, 3.621 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287586, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 260, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (287.15, 141.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 88, #reads = 1090400 mem = 536877790 peak = 1047580672
[M::process] read 690688 sequences (100000047 bp) in 2.560 CPU sec, 2.889 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287398, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 259, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (287.25, 141.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689620 reads in 170.913 CPU sec, 5.531 real sec
[M::mem_process_seqs] Processed 689686 reads in 111.925 CPU sec, 3.566 real sec
[M::process] read 689412 sequences (100000094 bp) in 2.229 CPU sec, 2.295 real sec...
sorting block 89, #reads = 1088969 mem = 536885912 peak = 1047580672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287803, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 252, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (279.40, 137.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688452 sequences (100000035 bp) in 2.703 CPU sec, 2.969 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286878, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 259, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (286.53, 140.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690688 reads in 173.339 CPU sec, 5.579 real sec
[M::mem_process_seqs] Processed 689412 reads in 110.939 CPU sec, 3.536 real sec
[M::process] read 688192 sequences (100000022 bp) in 2.099 CPU sec, 2.157 real sec...
sorting block 90, #reads = 1089481 mem = 536877883 peak = 1047580672
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287150, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 265, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (292.19, 143.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687668 sequences (100000096 bp) in 2.545 CPU sec, 2.820 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286978, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 266, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (292.80, 143.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688452 reads in 168.099 CPU sec, 5.429 real sec
[M::mem_process_seqs] Processed 688192 reads in 106.727 CPU sec, 3.410 real sec
sorting block 91, #reads = 1088465 mem = 536880629 peak = 1047580672
[M::process] read 687574 sequences (100000022 bp) in 2.221 CPU sec, 2.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286834, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 269, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (296.03, 145.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 92, #reads = 1087844 mem = 536882695 peak = 1063460864
[M::process] read 687646 sequences (100000091 bp) in 2.853 CPU sec, 3.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287153, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (297.43, 145.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687668 reads in 177.380 CPU sec, 5.766 real sec
[M::mem_process_seqs] Processed 687574 reads in 114.443 CPU sec, 3.738 real sec
[M::process] read 687584 sequences (100000091 bp) in 1.995 CPU sec, 2.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286927, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 269, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (296.38, 145.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 93, #reads = 1086687 mem = 536881295 peak = 1063460864
[M::process] read 689370 sequences (100000106 bp) in 2.637 CPU sec, 2.913 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287012, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 269, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (295.49, 144.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687646 reads in 173.181 CPU sec, 5.616 real sec
[M::mem_process_seqs] Processed 687584 reads in 111.161 CPU sec, 3.571 real sec
[M::process] read 686610 sequences (100000108 bp) in 2.151 CPU sec, 2.243 real sec...
sorting block 94, #reads = 1087859 mem = 536878102 peak = 1063460864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287169, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 256, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752)
[M::mem_pestat] mean and std.dev: (282.20, 136.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687022 sequences (100000009 bp) in 2.486 CPU sec, 2.950 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286762, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 273, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (299.17, 145.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689370 reads in 176.365 CPU sec, 5.743 real sec
[M::mem_process_seqs] Processed 686610 reads in 112.423 CPU sec, 3.625 real sec
[M::process] read 687434 sequences (100000132 bp) in 2.072 CPU sec, 2.288 real sec...
sorting block 95, #reads = 1086969 mem = 536879449 peak = 1063460864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286633, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 273, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (299.53, 147.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1035)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686150 sequences (100000266 bp) in 2.460 CPU sec, 2.799 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287304, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (297.23, 145.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687022 reads in 174.887 CPU sec, 5.648 real sec
sorting block 96, #reads = 1086414 mem = 536879318 peak = 1063460864
[M::mem_process_seqs] Processed 687434 reads in 112.872 CPU sec, 3.581 real sec
[M::process] read 685988 sequences (100000290 bp) in 2.063 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286519, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 278, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (302.94, 146.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 97, #reads = 1085996 mem = 536885216 peak = 1075441664
[M::process] read 685172 sequences (100000022 bp) in 2.626 CPU sec, 3.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286462, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 278, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (299.90, 142.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686150 reads in 177.526 CPU sec, 5.773 real sec
[M::mem_process_seqs] Processed 685988 reads in 116.993 CPU sec, 3.764 real sec
[M::process] read 684778 sequences (100000082 bp) in 2.152 CPU sec, 2.263 real sec...
sorting block 98, #reads = 1085523 mem = 536877836 peak = 1075441664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286017, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 283, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (304.59, 144.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684568 sequences (100000065 bp) in 2.228 CPU sec, 2.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286202, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 283, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (305.12, 144.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685172 reads in 179.490 CPU sec, 5.816 real sec
[M::process] read 683932 sequences (100000170 bp) in 2.146 CPU sec, 2.181 real sec...
[M::mem_process_seqs] Processed 684778 reads in 118.356 CPU sec, 3.791 real sec
sorting block 99, #reads = 1084250 mem = 536889795 peak = 1075441664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285921, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (304.21, 143.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684154 sequences (100000127 bp) in 2.611 CPU sec, 2.964 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285906, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 286, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (307.47, 144.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684568 reads in 178.397 CPU sec, 5.770 real sec
[M::process] read 683916 sequences (100000112 bp) in 2.066 CPU sec, 2.116 real sec...
[M::mem_process_seqs] Processed 683932 reads in 116.077 CPU sec, 3.704 real sec
sorting block 100, #reads = 1083304 mem = 536881240 peak = 1075441664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285749, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 285, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (306.29, 144.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683842 sequences (100000191 bp) in 2.477 CPU sec, 2.760 real sec...
sorting block 101, #reads = 1084587 mem = 536886253 peak = 1077866496
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285549, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (305.98, 143.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684154 reads in 176.474 CPU sec, 5.747 real sec
[M::process] read 683972 sequences (100000189 bp) in 1.910 CPU sec, 2.126 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286209, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (307.17, 144.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683842 reads in 99.510 CPU sec, 3.161 real sec
[M::process] read 684062 sequences (100000020 bp) in 2.846 CPU sec, 2.902 real sec...
sorting block 102, #reads = 1083804 mem = 536877477 peak = 1077866496
[M::mem_process_seqs] Processed 683916 reads in 270.567 CPU sec, 8.703 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285416, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 285, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (307.01, 145.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686214 sequences (100000191 bp) in 2.548 CPU sec, 2.854 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285990, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 285, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (308.46, 146.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683972 reads in 199.329 CPU sec, 6.364 real sec
sorting block 103, #reads = 1082638 mem = 536879002 peak = 1077866496
[M::mem_process_seqs] Processed 684062 reads in 118.885 CPU sec, 3.782 real sec
[M::process] read 694184 sequences (100000271 bp) in 2.501 CPU sec, 2.650 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286290, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (300.06, 144.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 104, #reads = 1083288 mem = 536877921 peak = 1086021632
[M::process] read 693822 sequences (100000012 bp) in 2.550 CPU sec, 3.032 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289320, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 241, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (266.30, 128.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686214 reads in 194.388 CPU sec, 6.319 real sec
[M::mem_process_seqs] Processed 694184 reads in 129.294 CPU sec, 4.134 real sec
[M::process] read 694724 sequences (100000024 bp) in 2.451 CPU sec, 2.522 real sec...
sorting block 105, #reads = 1082560 mem = 536879394 peak = 1086021632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 289252, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 243, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (268.11, 129.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 692546 sequences (100000260 bp) in 2.424 CPU sec, 2.777 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289309, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 240, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (265.21, 127.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691326 sequences (100000023 bp) in 2.127 CPU sec, 2.135 real sec...
[M::mem_process_seqs] Processed 693822 reads in 197.740 CPU sec, 6.377 real sec
[M::mem_process_seqs] Processed 694724 reads in 132.357 CPU sec, 4.245 real sec
sorting block 106, #reads = 1085167 mem = 536879441 peak = 1086021632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288326, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 248, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (273.62, 131.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 919)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690698 sequences (100000271 bp) in 2.795 CPU sec, 3.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288482, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 252, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743)
[M::mem_pestat] mean and std.dev: (277.42, 133.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 933)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690326 sequences (100000257 bp) in 2.106 CPU sec, 2.128 real sec...
[M::mem_process_seqs] Processed 692546 reads in 192.942 CPU sec, 6.225 real sec
sorting block 107, #reads = 1082793 mem = 536884145 peak = 1086021632
[M::mem_process_seqs] Processed 691326 reads in 122.784 CPU sec, 3.946 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288270, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 254, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753)
[M::mem_pestat] mean and std.dev: (279.93, 135.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 946)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 108, #reads = 1083967 mem = 536882951 peak = 1086021632
[M::process] read 689294 sequences (100000279 bp) in 2.445 CPU sec, 2.755 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 288810, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 438, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075)
[M::mem_pestat] mean and std.dev: (280.75, 158.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1392)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 257, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760)
[M::mem_pestat] mean and std.dev: (282.06, 136.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 955)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 688994 sequences (100000022 bp) in 1.905 CPU sec, 1.924 real sec...
[M::mem_process_seqs] Processed 690698 reads in 189.863 CPU sec, 6.142 real sec
[M::mem_process_seqs] Processed 690326 reads in 124.746 CPU sec, 4.025 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287735, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 262, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (286.79, 138.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 109, #reads = 1084969 mem = 536879195 peak = 1086021632
[M::process] read 688838 sequences (100000015 bp) in 2.935 CPU sec, 3.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288095, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 264, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (289.16, 139.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689294 reads in 179.797 CPU sec, 5.777 real sec
[M::process] read 688232 sequences (100000280 bp) in 2.005 CPU sec, 2.027 real sec...
[M::mem_process_seqs] Processed 688994 reads in 115.696 CPU sec, 3.687 real sec
sorting block 110, #reads = 1085367 mem = 536879642 peak = 1086021632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287946, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 264, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (289.91, 140.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688856 sequences (100000061 bp) in 2.700 CPU sec, 3.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287488, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 266, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (291.31, 140.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688838 reads in 188.089 CPU sec, 6.091 real sec
[M::process] read 688030 sequences (100000058 bp) in 1.925 CPU sec, 1.964 real sec...
[M::mem_process_seqs] Processed 688232 reads in 127.787 CPU sec, 4.095 real sec
sorting block 111, #reads = 1086114 mem = 536877405 peak = 1086021632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287801, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 265, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (290.23, 140.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687812 sequences (100000236 bp) in 2.634 CPU sec, 2.950 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287285, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 268, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (292.12, 140.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687398 sequences (100000087 bp) in 2.066 CPU sec, 2.071 real sec...
[M::mem_process_seqs] Processed 688856 reads in 185.288 CPU sec, 5.952 real sec
sorting block 112, #reads = 1082043 mem = 536879103 peak = 1086021632
[M::mem_process_seqs] Processed 688030 reads in 119.353 CPU sec, 3.804 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287460, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 267, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (291.50, 139.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 113, #reads = 1083686 mem = 536885543 peak = 1091883008
[M::process] read 687014 sequences (100000245 bp) in 2.608 CPU sec, 2.985 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287557, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (293.61, 140.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687812 reads in 181.398 CPU sec, 5.900 real sec
[M::process] read 687292 sequences (100000152 bp) in 2.112 CPU sec, 2.145 real sec...
[M::mem_process_seqs] Processed 687398 reads in 118.960 CPU sec, 3.842 real sec
sorting block 114, #reads = 1084755 mem = 536879266 peak = 1091883008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287056, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (296.50, 142.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687462 sequences (100000260 bp) in 2.637 CPU sec, 2.971 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287274, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (295.96, 142.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687014 reads in 181.614 CPU sec, 5.853 real sec
[M::process] read 686648 sequences (100000245 bp) in 1.950 CPU sec, 2.089 real sec...
[M::mem_process_seqs] Processed 687292 reads in 116.922 CPU sec, 3.734 real sec
sorting block 115, #reads = 1083653 mem = 536880788 peak = 1091883008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286973, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (295.45, 142.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 290, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (161, 376)
[M::mem_pestat] mean and std.dev: (270.45, 21.41)
[M::mem_pestat] low and high boundaries for proper pairs: (118, 419)
[M::mem_pestat] skip orientation RR
[M::process] read 685714 sequences (100000230 bp) in 2.442 CPU sec, 2.854 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286619, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (299.16, 144.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687462 reads in 178.780 CPU sec, 5.847 real sec
[M::mem_process_seqs] Processed 686648 reads in 113.263 CPU sec, 3.685 real sec
[M::process] read 685326 sequences (100000134 bp) in 2.690 CPU sec, 2.884 real sec...
sorting block 116, #reads = 1084713 mem = 536877606 peak = 1091883008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286011, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (303.03, 145.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684606 sequences (100000206 bp) in 2.877 CPU sec, 3.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286247, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (306.13, 147.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1042)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685714 reads in 176.975 CPU sec, 5.741 real sec
sorting block 117, #reads = 1084958 mem = 536885148 peak = 1091883008
[M::mem_process_seqs] Processed 685326 reads in 112.978 CPU sec, 3.661 real sec
[M::process] read 684940 sequences (100000286 bp) in 2.185 CPU sec, 2.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285959, 8, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (306.82, 146.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (426, 570, 570)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (138, 858)
[M::mem_pestat] mean and std.dev: (510.91, 110.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RR
sorting block 118, #reads = 1084983 mem = 536885110 peak = 1091883008
[M::process] read 684914 sequences (100000109 bp) in 2.750 CPU sec, 3.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286205, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (305.33, 146.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684606 reads in 180.139 CPU sec, 5.849 real sec
[M::mem_process_seqs] Processed 684940 reads in 115.210 CPU sec, 3.722 real sec
[M::process] read 684806 sequences (100000186 bp) in 2.292 CPU sec, 2.411 real sec...
sorting block 119, #reads = 1083811 mem = 536878974 peak = 1091883008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286258, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (305.54, 147.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684946 sequences (100000269 bp) in 2.647 CPU sec, 2.927 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286491, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (305.40, 147.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684914 reads in 178.321 CPU sec, 5.726 real sec
[M::mem_process_seqs] Processed 684806 reads in 117.208 CPU sec, 3.720 real sec
[M::process] read 685822 sequences (100000284 bp) in 2.257 CPU sec, 2.361 real sec...
sorting block 120, #reads = 1084420 mem = 536878717 peak = 1091883008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286143, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825)
[M::mem_pestat] mean and std.dev: (305.39, 147.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685074 sequences (100000048 bp) in 2.568 CPU sec, 2.856 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285624, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 273, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (298.74, 144.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684946 reads in 180.675 CPU sec, 5.838 real sec
[M::mem_process_seqs] Processed 685822 reads in 119.672 CPU sec, 3.810 real sec
[M::process] read 688266 sequences (100000226 bp) in 2.241 CPU sec, 2.293 real sec...
sorting block 121, #reads = 1084264 mem = 536877905 peak = 1091883008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285585, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (304.19, 148.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1036)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 122, #reads = 1083732 mem = 536879310 peak = 1091883008
[M::process] read 691836 sequences (100000198 bp) in 2.689 CPU sec, 2.952 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286977, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 264, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (291.53, 142.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691992 sequences (100000232 bp) in 1.927 CPU sec, 1.956 real sec...
[M::mem_process_seqs] Processed 685074 reads in 201.671 CPU sec, 6.521 real sec
[M::mem_process_seqs] Processed 688266 reads in 138.523 CPU sec, 4.446 real sec
sorting block 123, #reads = 1079551 mem = 536882258 peak = 1091883008
[M::process] read 690958 sequences (100000104 bp) in 2.583 CPU sec, 2.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288667, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 250, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (275.71, 133.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689690 sequences (100000124 bp) in 1.888 CPU sec, 1.953 real sec...
[M::mem_process_seqs] Processed 691992 reads in 99.088 CPU sec, 3.189 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288450, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 249, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (275.08, 133.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 920)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288259, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 254, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (279.88, 136.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 691836 reads in 275.471 CPU sec, 8.884 real sec
sorting block 124, #reads = 1082712 mem = 536879515 peak = 1091883008
[M::mem_process_seqs] Processed 690958 reads in 129.592 CPU sec, 4.159 real sec
[M::process] read 689146 sequences (100000038 bp) in 2.499 CPU sec, 2.614 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287903, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 260, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (284.58, 137.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 957)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 125, #reads = 1089406 mem = 536880726 peak = 1100283904
[M::process] read 688636 sequences (100000212 bp) in 2.883 CPU sec, 3.276 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287329, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 262, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (286.73, 138.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689690 reads in 170.414 CPU sec, 5.500 real sec
[M::mem_process_seqs] Processed 689146 reads in 108.861 CPU sec, 3.473 real sec
[M::process] read 688894 sequences (100000042 bp) in 2.166 CPU sec, 2.282 real sec...
sorting block 126, #reads = 1088391 mem = 536880300 peak = 1100283904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287637, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 264, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (288.81, 139.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688388 sequences (100000204 bp) in 2.682 CPU sec, 3.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287354, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 263, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (288.25, 139.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688636 reads in 170.809 CPU sec, 5.523 real sec
[M::mem_process_seqs] Processed 688894 reads in 109.010 CPU sec, 3.499 real sec
[M::process] read 688108 sequences (100000139 bp) in 2.410 CPU sec, 2.597 real sec...
sorting block 127, #reads = 1087852 mem = 536878106 peak = 1100283904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287659, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 266, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (290.54, 140.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687736 sequences (100000257 bp) in 2.684 CPU sec, 3.068 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287180, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (292.54, 141.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688388 reads in 171.591 CPU sec, 5.580 real sec
[M::mem_process_seqs] Processed 688108 reads in 113.013 CPU sec, 3.643 real sec
sorting block 128, #reads = 1089004 mem = 536879283 peak = 1100283904
[M::process] read 687460 sequences (100000013 bp) in 2.235 CPU sec, 2.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287292, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 270, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (294.55, 142.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 129, #reads = 1088856 mem = 536878214 peak = 1100283904
[M::process] read 687186 sequences (100000136 bp) in 2.710 CPU sec, 3.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287009, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (294.81, 141.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687736 reads in 171.386 CPU sec, 5.569 real sec
[M::mem_process_seqs] Processed 687460 reads in 114.599 CPU sec, 3.685 real sec
[M::process] read 687358 sequences (100000162 bp) in 2.520 CPU sec, 2.821 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287158, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 273, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (296.99, 143.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 130, #reads = 1086933 mem = 536880143 peak = 1100283904
[M::process] read 686990 sequences (100000190 bp) in 2.656 CPU sec, 3.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287035, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 272, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (296.59, 143.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687186 reads in 171.981 CPU sec, 5.614 real sec
[M::mem_process_seqs] Processed 687358 reads in 114.563 CPU sec, 3.701 real sec
[M::process] read 686742 sequences (100000172 bp) in 2.562 CPU sec, 2.779 real sec...
sorting block 131, #reads = 1086835 mem = 536879912 peak = 1100283904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286446, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (297.19, 143.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686494 sequences (100000289 bp) in 2.630 CPU sec, 2.803 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286868, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 275, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (298.56, 143.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686990 reads in 173.288 CPU sec, 5.632 real sec
[M::mem_process_seqs] Processed 686742 reads in 110.261 CPU sec, 3.576 real sec
sorting block 132, #reads = 1085911 mem = 536881542 peak = 1100283904
[M::process] read 686618 sequences (100000008 bp) in 2.500 CPU sec, 2.789 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286411, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (300.36, 145.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686272 sequences (100000215 bp) in 2.375 CPU sec, 2.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286279, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 275, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (300.31, 145.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686494 reads in 173.588 CPU sec, 5.637 real sec
[M::process] read 686250 sequences (100000088 bp) in 1.738 CPU sec, 1.779 real sec...
sorting block 133, #reads = 1086377 mem = 536877402 peak = 1100283904
[M::mem_process_seqs] Processed 686618 reads in 110.678 CPU sec, 3.628 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286540, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 277, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (301.39, 145.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 134, #reads = 1085833 mem = 536878314 peak = 1100283904
[M::process] read 685662 sequences (100000144 bp) in 2.993 CPU sec, 3.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286213, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (299.76, 144.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686272 reads in 178.877 CPU sec, 5.798 real sec
[M::mem_process_seqs] Processed 686250 reads in 113.127 CPU sec, 3.651 real sec
[M::process] read 685660 sequences (100000250 bp) in 2.195 CPU sec, 2.262 real sec...
sorting block 135, #reads = 1085193 mem = 536878150 peak = 1100283904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286363, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 278, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (301.06, 143.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685244 sequences (100000222 bp) in 2.816 CPU sec, 3.003 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286130, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (299.54, 141.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685378 sequences (100000052 bp) in 1.857 CPU sec, 1.858 real sec...
[M::mem_process_seqs] Processed 685662 reads in 180.513 CPU sec, 5.812 real sec
[M::mem_process_seqs] Processed 685660 reads in 117.351 CPU sec, 3.755 real sec
sorting block 136, #reads = 1084813 mem = 536879348 peak = 1100283904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286439, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (301.30, 143.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685744 sequences (100000198 bp) in 2.689 CPU sec, 3.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286581, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (300.80, 143.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685954 sequences (100000118 bp) in 1.970 CPU sec, 1.979 real sec...
[M::mem_process_seqs] Processed 685244 reads in 180.309 CPU sec, 5.811 real sec
[M::mem_process_seqs] Processed 685378 reads in 116.939 CPU sec, 3.721 real sec
sorting block 137, #reads = 1084071 mem = 536880050 peak = 1100283904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286161, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (298.61, 142.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685812 sequences (100000297 bp) in 2.982 CPU sec, 3.410 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286713, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (298.81, 142.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685744 reads in 181.314 CPU sec, 5.828 real sec
sorting block 138, #reads = 1082942 mem = 536876452 peak = 1100283904
[M::process] read 685952 sequences (100000233 bp) in 2.187 CPU sec, 2.326 real sec...
[M::mem_process_seqs] Processed 685954 reads in 117.993 CPU sec, 3.764 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286294, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (300.01, 144.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 139, #reads = 1083697 mem = 536878432 peak = 1100283904
[M::process] read 685826 sequences (100000090 bp) in 3.383 CPU sec, 3.859 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286650, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (299.15, 143.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685812 reads in 183.849 CPU sec, 5.965 real sec
[M::mem_process_seqs] Processed 685952 reads in 119.293 CPU sec, 3.851 real sec
[M::process] read 685470 sequences (100000071 bp) in 2.431 CPU sec, 2.628 real sec...
sorting block 140, #reads = 1084168 mem = 536878956 peak = 1100283904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286102, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (299.82, 144.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685918 sequences (100000141 bp) in 2.921 CPU sec, 3.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285800, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (299.77, 144.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685826 reads in 183.797 CPU sec, 5.963 real sec
[M::mem_process_seqs] Processed 685470 reads in 120.718 CPU sec, 3.891 real sec
[M::process] read 689332 sequences (100000027 bp) in 2.112 CPU sec, 2.300 real sec...
sorting block 141, #reads = 1082331 mem = 536879687 peak = 1100283904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285890, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 274, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (299.44, 144.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695716 sequences (100000215 bp) in 3.084 CPU sec, 3.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286282, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 260, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (286.35, 139.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 966)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685918 reads in 211.753 CPU sec, 6.794 real sec
[M::process] read 695220 sequences (100000169 bp) in 2.109 CPU sec, 2.217 real sec...
sorting block 142, #reads = 1080998 mem = 536879426 peak = 1100283904
[M::mem_process_seqs] Processed 689332 reads in 143.564 CPU sec, 4.569 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 289730, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 236, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (261.69, 126.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 143, #reads = 1073348 mem = 536878383 peak = 1100283904
[M::process] read 694172 sequences (100000083 bp) in 3.443 CPU sec, 3.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 290069, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 239, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (264.27, 128.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 881)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695716 reads in 187.254 CPU sec, 5.944 real sec
[M::process] read 692810 sequences (100000273 bp) in 2.372 CPU sec, 2.405 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 289676, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 242, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710)
[M::mem_pestat] mean and std.dev: (267.32, 129.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 694172 reads in 104.173 CPU sec, 3.297 real sec
[M::process] read 691748 sequences (100000035 bp) in 2.393 CPU sec, 2.401 real sec...
sorting block 144, #reads = 1084661 mem = 536878292 peak = 1100283904
[M::mem_process_seqs] Processed 695220 reads in 255.186 CPU sec, 8.068 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288745, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 248, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731)
[M::mem_pestat] mean and std.dev: (273.36, 132.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 145, #reads = 1083840 mem = 536879889 peak = 1100283904
[M::process] read 690962 sequences (100000115 bp) in 3.162 CPU sec, 3.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288619, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 251, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (276.86, 134.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 692810 reads in 209.055 CPU sec, 6.654 real sec
[M::mem_process_seqs] Processed 691748 reads in 128.033 CPU sec, 4.070 real sec
[M::process] read 690278 sequences (100000232 bp) in 2.766 CPU sec, 2.896 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288421, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 255, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (280.18, 135.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 943)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 146, #reads = 1083323 mem = 536876750 peak = 1100283904
[M::process] read 689198 sequences (100000147 bp) in 2.826 CPU sec, 3.223 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288302, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 258, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (283.39, 136.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690962 reads in 194.157 CPU sec, 6.250 real sec
[M::mem_process_seqs] Processed 690278 reads in 125.777 CPU sec, 3.995 real sec
[M::process] read 689104 sequences (100000133 bp) in 2.727 CPU sec, 2.833 real sec...
sorting block 147, #reads = 1084558 mem = 536880456 peak = 1100283904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287617, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 264, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (288.13, 138.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688690 sequences (100000020 bp) in 3.243 CPU sec, 3.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287929, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 264, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (288.86, 139.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689198 reads in 189.183 CPU sec, 6.076 real sec
[M::mem_process_seqs] Processed 689104 reads in 122.324 CPU sec, 3.883 real sec
[M::process] read 687590 sequences (100000257 bp) in 2.125 CPU sec, 2.193 real sec...
sorting block 148, #reads = 1085279 mem = 536878153 peak = 1100283904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287521, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (291.21, 140.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688134 sequences (100000065 bp) in 2.884 CPU sec, 3.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287241, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (295.16, 142.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688690 reads in 174.570 CPU sec, 5.563 real sec
[M::mem_process_seqs] Processed 687590 reads in 114.305 CPU sec, 3.605 real sec
sorting block 149, #reads = 1084169 mem = 536892596 peak = 1100283904
[M::process] read 687714 sequences (100000003 bp) in 2.543 CPU sec, 2.638 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287515, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 269, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (293.74, 142.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 150, #reads = 1083712 mem = 536881967 peak = 1100283904
[M::process] read 687792 sequences (100000182 bp) in 3.366 CPU sec, 3.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287278, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (295.55, 143.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688134 reads in 193.950 CPU sec, 6.205 real sec
[M::mem_process_seqs] Processed 687714 reads in 128.080 CPU sec, 4.055 real sec
[M::process] read 687026 sequences (100000190 bp) in 2.708 CPU sec, 2.842 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287247, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 269, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (295.36, 143.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 151, #reads = 1082552 mem = 536879203 peak = 1100283904
[M::process] read 687150 sequences (100000241 bp) in 2.960 CPU sec, 3.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287148, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 270, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (294.96, 141.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687792 reads in 196.292 CPU sec, 6.312 real sec
[M::mem_process_seqs] Processed 687026 reads in 127.934 CPU sec, 4.059 real sec
[M::process] read 686748 sequences (100000086 bp) in 2.932 CPU sec, 3.091 real sec...
sorting block 152, #reads = 1083007 mem = 536878066 peak = 1100283904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286894, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 270, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (294.65, 141.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687170 sequences (100000292 bp) in 3.367 CPU sec, 3.778 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286599, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 271, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (296.83, 143.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687150 reads in 194.613 CPU sec, 6.240 real sec
[M::mem_process_seqs] Processed 686748 reads in 126.046 CPU sec, 4.007 real sec
sorting block 153, #reads = 1082020 mem = 536880563 peak = 1100283904
[M::process] read 686896 sequences (100000206 bp) in 2.800 CPU sec, 3.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286766, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (295.02, 142.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686706 sequences (100000182 bp) in 2.640 CPU sec, 2.858 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286509, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (294.30, 142.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687170 reads in 189.337 CPU sec, 6.073 real sec
sorting block 154, #reads = 1083544 mem = 536878310 peak = 1100283904
[M::mem_process_seqs] Processed 686896 reads in 119.022 CPU sec, 3.768 real sec
[M::process] read 686414 sequences (100000006 bp) in 2.250 CPU sec, 2.316 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286842, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 271, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (296.50, 143.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 155, #reads = 1083995 mem = 536879610 peak = 1100283904
[M::process] read 686266 sequences (100000284 bp) in 2.225 CPU sec, 2.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286700, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 272, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (297.46, 143.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686186 sequences (100000073 bp) in 1.460 CPU sec, 1.454 real sec...
[M::mem_process_seqs] Processed 686706 reads in 151.097 CPU sec, 4.806 real sec
[M::mem_process_seqs] Processed 686414 reads in 95.893 CPU sec, 3.045 real sec
sorting block 156, #reads = 1085392 mem = 536877467 peak = 1100283904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286180, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 273, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (297.64, 142.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685856 sequences (100000283 bp) in 2.336 CPU sec, 2.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286210, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 273, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (296.97, 142.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686266 reads in 152.535 CPU sec, 4.842 real sec
[M::process] read 685890 sequences (100000018 bp) in 2.026 CPU sec, 2.043 real sec...
[M::mem_process_seqs] Processed 686186 reads in 97.120 CPU sec, 3.059 real sec
sorting block 157, #reads = 1085042 mem = 536878547 peak = 1100283904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286671, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 273, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (298.55, 143.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685506 sequences (100000089 bp) in 2.798 CPU sec, 2.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286237, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (299.98, 145.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685856 reads in 154.752 CPU sec, 4.915 real sec
[M::mem_process_seqs] Processed 685890 reads in 99.943 CPU sec, 3.144 real sec
[M::process] read 685244 sequences (100000216 bp) in 2.070 CPU sec, 2.105 real sec...
sorting block 158, #reads = 1085826 mem = 536878389 peak = 1100283904
sorting block 159, #reads = 1085774 mem = 536883072 peak = 1100283904
[M::process] read 685374 sequences (100000263 bp) in 2.613 CPU sec, 2.772 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286657, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (302.99, 146.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 494496 sequences (72048829 bp) in 1.340 CPU sec, 1.338 real sec...
[M::mem_process_seqs] Processed 685244 reads in 91.757 CPU sec, 2.897 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286186, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (301.52, 145.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286504, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 279, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (304.03, 147.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685506 reads in 247.278 CPU sec, 7.922 real sec
[M::mem_process_seqs] Processed 685374 reads in 116.036 CPU sec, 3.752 real sec
sorting block 160, #reads = 1085686 mem = 536877937 peak = 1100283904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 206246, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 273, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (299.39, 145.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 494496 reads in 67.315 CPU sec, 2.234 real sec
sorting block 161, #reads = 1085485 mem = 536877214 peak = 1103785984
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 175540372
overall: 69807898624 mem 25734.773 user 648.547 sys 909.658 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_cfy-T1-TDNA-1_A23MHWWLT4_1.L003\tPU:L003\tSM:659_cfy-T1-TDNA-1_A23MHWWLT4_1\tLB:659_cfy-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_cfy-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_cfy-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 909.695 sec; CPU: 26383.323 sec
sorting block 162, #reads = 433751 mem = 215208388 peak = 1103785984
uniq header count 1
merging 163 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 9242710086
output reads: 176414291
bam_mem_sort: 163 calls 115.125 user 3.399 sys 122.872 real
bam_write: 163 calls 100.440 user 7.198 sys 213.289 real
execute: 1 calls 44.160 user 1.376 sys 963.075 real
merge_files: 1 calls 44.136 user 1.347 sys 52.945 real
parse_chunk: 2599 calls 117.942 user 33.509 sys 201.155 real
read_chunk: 7007 calls 55.849 user 40.548 sys 836.331 real
sort_block: 1 calls 40.809 user 39.904 sys 814.042 real
write_chunk: 4406 calls 43.806 user 1.060 sys 52.025 real
overall: 3225841664 mem 2200.517 user 105.201 sys 963.097 real
ls: cannot access '*.cram': No such file or directory