cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_Kh-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2036.288 CPU sec, 76.185 real sec
[M::process] read 672518 sequences (100000058 bp) in 1.182 CPU sec, 1.183 real sec...
[M::process] read 672126 sequences (100000296 bp) in 1.500 CPU sec, 1.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278157, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 364, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (380.51, 164.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671986 sequences (100000229 bp) in 1.563 CPU sec, 1.572 real sec...
[M::process] read 671688 sequences (100000138 bp) in 1.571 CPU sec, 1.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278185, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 367, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (383.27, 164.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672518 reads in 183.744 CPU sec, 5.784 real sec
[M::mem_process_seqs] Processed 672126 reads in 114.217 CPU sec, 3.630 real sec
sorting block 0, #reads = 1075955 mem = 536878763 peak = 585674752
[M::process] read 671270 sequences (100000124 bp) in 1.575 CPU sec, 1.758 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278017, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 369, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (385.23, 165.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671214 sequences (100000287 bp) in 1.419 CPU sec, 1.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277671, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 372, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (387.15, 164.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277941, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 375, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (390.08, 165.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671986 reads in 249.881 CPU sec, 8.077 real sec
[M::mem_process_seqs] Processed 671688 reads in 187.980 CPU sec, 6.131 real sec
[M::mem_process_seqs] Processed 671270 reads in 203.653 CPU sec, 6.666 real sec
sorting block 1, #reads = 1075050 mem = 536877521 peak = 713076736
[M::process] read 671072 sequences (100000229 bp) in 2.018 CPU sec, 2.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277723, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 374, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (389.27, 164.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 2, #reads = 1075297 mem = 536876987 peak = 885792768
[M::process] read 670838 sequences (100000041 bp) in 1.974 CPU sec, 2.036 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278178, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 380, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (394.00, 165.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671214 reads in 185.182 CPU sec, 5.908 real sec
[M::process] read 670604 sequences (100000204 bp) in 1.730 CPU sec, 1.766 real sec...
[M::mem_process_seqs] Processed 671072 reads in 118.418 CPU sec, 3.755 real sec
sorting block 3, #reads = 1074560 mem = 536878758 peak = 885792768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277859, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 384, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (397.56, 167.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670766 sequences (100000103 bp) in 1.983 CPU sec, 2.227 real sec...
[M::process] read 670648 sequences (100000008 bp) in 1.672 CPU sec, 1.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277875, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 386, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (399.79, 167.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670838 reads in 196.643 CPU sec, 6.527 real sec
[M::mem_process_seqs] Processed 670604 reads in 125.268 CPU sec, 4.109 real sec
sorting block 4, #reads = 1074975 mem = 536880046 peak = 885792768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277482, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 384, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (397.24, 166.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670728 sequences (100000197 bp) in 2.201 CPU sec, 2.539 real sec...
[M::process] read 670582 sequences (100000217 bp) in 1.575 CPU sec, 1.585 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277100, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 383, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (396.78, 165.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670766 reads in 209.506 CPU sec, 7.018 real sec
[M::mem_process_seqs] Processed 670648 reads in 129.479 CPU sec, 4.314 real sec
sorting block 5, #reads = 1075172 mem = 536876489 peak = 885792768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278127, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 383, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (397.57, 166.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670730 sequences (100000170 bp) in 2.409 CPU sec, 2.847 real sec...
[M::process] read 670366 sequences (100000283 bp) in 1.726 CPU sec, 1.832 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277984, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 386, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (400.72, 169.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670728 reads in 212.834 CPU sec, 7.244 real sec
sorting block 6, #reads = 1074843 mem = 536877577 peak = 885792768
[M::mem_process_seqs] Processed 670582 reads in 132.232 CPU sec, 4.500 real sec
sorting block 7, #reads = 1073827 mem = 536877929 peak = 885792768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277665, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (394.85, 166.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670448 sequences (100000091 bp) in 2.136 CPU sec, 2.440 real sec...
[M::process] read 670370 sequences (100000192 bp) in 1.705 CPU sec, 1.763 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 277598, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 389, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (402.31, 167.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670730 reads in 213.070 CPU sec, 7.275 real sec
[M::mem_process_seqs] Processed 670366 reads in 130.432 CPU sec, 4.418 real sec
sorting block 8, #reads = 1075470 mem = 536879529 peak = 885792768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277154, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 381, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (395.01, 164.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670512 sequences (100000132 bp) in 2.678 CPU sec, 2.916 real sec...
[M::process] read 670556 sequences (100000134 bp) in 1.736 CPU sec, 1.759 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 276209, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 381, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (395.22, 164.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670448 reads in 214.700 CPU sec, 7.310 real sec
[M::mem_process_seqs] Processed 670370 reads in 128.602 CPU sec, 4.311 real sec
sorting block 9, #reads = 1075021 mem = 536878276 peak = 885792768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277101, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 376, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (391.58, 163.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670422 sequences (100000248 bp) in 2.060 CPU sec, 2.431 real sec...
[M::process] read 670724 sequences (100000078 bp) in 1.718 CPU sec, 1.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277035, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 377, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (391.61, 162.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670512 reads in 208.480 CPU sec, 6.965 real sec
[M::mem_process_seqs] Processed 670556 reads in 129.919 CPU sec, 4.269 real sec
sorting block 10, #reads = 1073562 mem = 536880289 peak = 885792768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277196, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 380, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (394.28, 163.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670692 sequences (100000070 bp) in 2.602 CPU sec, 3.000 real sec...
[M::process] read 670650 sequences (100000295 bp) in 1.695 CPU sec, 1.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277027, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 374, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (389.73, 162.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670422 reads in 208.074 CPU sec, 6.871 real sec
sorting block 11, #reads = 1074369 mem = 536879057 peak = 885792768
[M::mem_process_seqs] Processed 670724 reads in 130.965 CPU sec, 4.284 real sec
sorting block 12, #reads = 1072888 mem = 536881232 peak = 911200256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277241, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 376, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (390.85, 163.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670700 sequences (100000039 bp) in 2.369 CPU sec, 2.817 real sec...
[M::process] read 670626 sequences (100000006 bp) in 1.884 CPU sec, 1.887 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277109, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (392.63, 164.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670692 reads in 207.684 CPU sec, 6.839 real sec
[M::mem_process_seqs] Processed 670650 reads in 127.191 CPU sec, 4.164 real sec
sorting block 13, #reads = 1073770 mem = 536879072 peak = 911200256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277363, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (392.42, 164.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670524 sequences (100000107 bp) in 2.715 CPU sec, 3.153 real sec...
[M::process] read 670276 sequences (100000098 bp) in 1.785 CPU sec, 1.835 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277812, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 377, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (392.71, 165.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670700 reads in 204.278 CPU sec, 6.695 real sec
[M::mem_process_seqs] Processed 670626 reads in 124.963 CPU sec, 4.093 real sec
sorting block 14, #reads = 1073274 mem = 536877942 peak = 911200256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277486, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 383, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (399.07, 168.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670302 sequences (100000183 bp) in 2.320 CPU sec, 2.754 real sec...
[M::process] read 672278 sequences (100000221 bp) in 1.903 CPU sec, 1.907 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277673, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 388, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (403.74, 171.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (5265, 5720, 5741)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4313, 6693)
[M::mem_pestat] mean and std.dev: (5616.75, 203.26)
[M::mem_pestat] low and high boundaries for proper pairs: (3837, 7169)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 670524 reads in 194.864 CPU sec, 6.336 real sec
[M::mem_process_seqs] Processed 670276 reads in 123.468 CPU sec, 3.971 real sec
sorting block 15, #reads = 1073737 mem = 536879516 peak = 911200256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277643, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 389, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (404.84, 172.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671998 sequences (100000187 bp) in 2.615 CPU sec, 3.040 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278094, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 363, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (380.99, 165.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671704 sequences (100000141 bp) in 1.731 CPU sec, 1.736 real sec...
[M::mem_process_seqs] Processed 670302 reads in 189.991 CPU sec, 6.177 real sec
sorting block 16, #reads = 1072358 mem = 536877023 peak = 911200256
[M::mem_process_seqs] Processed 672278 reads in 121.660 CPU sec, 3.942 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278250, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 367, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (384.26, 165.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 17, #reads = 1073879 mem = 536879630 peak = 911958016
[M::process] read 671696 sequences (100000127 bp) in 2.217 CPU sec, 2.593 real sec...
[M::process] read 671506 sequences (100000012 bp) in 1.935 CPU sec, 1.941 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277452, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 367, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (384.07, 164.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671998 reads in 188.558 CPU sec, 6.104 real sec
[M::mem_process_seqs] Processed 671704 reads in 119.629 CPU sec, 3.847 real sec
sorting block 18, #reads = 1076560 mem = 536882063 peak = 911958016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278073, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 369, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (384.02, 162.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671072 sequences (100000077 bp) in 2.691 CPU sec, 2.964 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277506, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 369, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (385.09, 163.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671150 sequences (100000047 bp) in 1.812 CPU sec, 1.844 real sec...
[M::mem_process_seqs] Processed 671696 reads in 186.718 CPU sec, 6.012 real sec
[M::mem_process_seqs] Processed 671506 reads in 117.504 CPU sec, 3.755 real sec
sorting block 19, #reads = 1077588 mem = 536879687 peak = 911958016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 277910, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 372, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (388.03, 163.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670972 sequences (100000018 bp) in 2.420 CPU sec, 2.825 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277554, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 374, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (389.87, 164.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670908 sequences (100000065 bp) in 1.790 CPU sec, 1.808 real sec...
[M::mem_process_seqs] Processed 671072 reads in 186.951 CPU sec, 6.101 real sec
[M::mem_process_seqs] Processed 671150 reads in 119.662 CPU sec, 3.896 real sec
sorting block 20, #reads = 1075683 mem = 536880815 peak = 911958016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277615, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 378, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (392.12, 164.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670782 sequences (100000005 bp) in 2.681 CPU sec, 3.036 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277760, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 381, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (394.45, 165.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670710 sequences (100000062 bp) in 1.728 CPU sec, 1.839 real sec...
[M::mem_process_seqs] Processed 670972 reads in 183.458 CPU sec, 5.963 real sec
sorting block 21, #reads = 1075040 mem = 536879575 peak = 911958016
[M::mem_process_seqs] Processed 670908 reads in 116.390 CPU sec, 3.767 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277141, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 380, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (394.35, 165.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 22, #reads = 1075378 mem = 536878694 peak = 953434112
[M::process] read 670740 sequences (100000122 bp) in 2.452 CPU sec, 2.882 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277313, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 384, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (397.47, 167.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670552 sequences (100000125 bp) in 1.851 CPU sec, 1.885 real sec...
[M::mem_process_seqs] Processed 670782 reads in 186.789 CPU sec, 6.114 real sec
[M::mem_process_seqs] Processed 670710 reads in 117.889 CPU sec, 3.839 real sec
sorting block 23, #reads = 1075012 mem = 536880086 peak = 953434112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277251, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 383, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (397.08, 166.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670468 sequences (100000180 bp) in 2.837 CPU sec, 3.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277475, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 382, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (395.03, 164.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670476 sequences (100000027 bp) in 1.655 CPU sec, 1.861 real sec...
[M::mem_process_seqs] Processed 670740 reads in 191.008 CPU sec, 6.181 real sec
[M::mem_process_seqs] Processed 670552 reads in 118.624 CPU sec, 3.858 real sec
sorting block 24, #reads = 1075937 mem = 536880279 peak = 953434112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277756, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 386, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (400.69, 168.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670632 sequences (100000159 bp) in 2.518 CPU sec, 2.845 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278436, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 377, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (391.86, 163.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670362 sequences (100000288 bp) in 1.830 CPU sec, 1.883 real sec...
[M::mem_process_seqs] Processed 670468 reads in 191.092 CPU sec, 6.164 real sec
[M::mem_process_seqs] Processed 670476 reads in 116.292 CPU sec, 3.768 real sec
sorting block 25, #reads = 1074951 mem = 536877637 peak = 953434112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278249, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 382, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (395.90, 166.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670342 sequences (100000293 bp) in 2.959 CPU sec, 3.281 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277411, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 384, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (398.11, 165.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670278 sequences (100000117 bp) in 1.792 CPU sec, 1.797 real sec...
[M::mem_process_seqs] Processed 670632 reads in 188.387 CPU sec, 6.072 real sec
sorting block 26, #reads = 1075513 mem = 536881035 peak = 953434112
[M::mem_process_seqs] Processed 670362 reads in 118.595 CPU sec, 3.777 real sec
sorting block 27, #reads = 1074039 mem = 536877932 peak = 989851648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277854, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 382, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (395.93, 164.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670772 sequences (100000175 bp) in 2.522 CPU sec, 2.820 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277220, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 385, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (398.73, 166.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670462 sequences (100000013 bp) in 1.958 CPU sec, 1.970 real sec...
[M::mem_process_seqs] Processed 670342 reads in 192.766 CPU sec, 6.211 real sec
[M::mem_process_seqs] Processed 670278 reads in 122.075 CPU sec, 3.929 real sec
sorting block 28, #reads = 1074524 mem = 536879889 peak = 989851648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278216, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (392.42, 167.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670782 sequences (100000236 bp) in 2.772 CPU sec, 3.208 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277658, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 382, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (396.51, 166.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670648 sequences (100000066 bp) in 1.770 CPU sec, 1.778 real sec...
[M::mem_process_seqs] Processed 670772 reads in 190.909 CPU sec, 6.167 real sec
[M::mem_process_seqs] Processed 670462 reads in 123.381 CPU sec, 3.956 real sec
sorting block 29, #reads = 1074132 mem = 536877043 peak = 989851648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277329, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (393.89, 166.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670612 sequences (100000104 bp) in 2.362 CPU sec, 3.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277222, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (394.72, 167.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670620 sequences (100000243 bp) in 2.028 CPU sec, 2.113 real sec...
[M::mem_process_seqs] Processed 670782 reads in 194.224 CPU sec, 6.427 real sec
[M::mem_process_seqs] Processed 670648 reads in 124.328 CPU sec, 4.035 real sec
sorting block 30, #reads = 1074068 mem = 536885305 peak = 989851648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277502, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (393.64, 166.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670492 sequences (100000048 bp) in 2.779 CPU sec, 3.242 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277973, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (394.14, 166.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670612 reads in 188.476 CPU sec, 6.092 real sec
[M::process] read 670482 sequences (100000022 bp) in 1.791 CPU sec, 1.943 real sec...
sorting block 31, #reads = 1073396 mem = 536884960 peak = 989851648
[M::mem_process_seqs] Processed 670620 reads in 119.714 CPU sec, 3.841 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277910, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (398.38, 169.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 32, #reads = 1074738 mem = 536877447 peak = 1037168640
[M::process] read 670606 sequences (100000273 bp) in 2.370 CPU sec, 2.823 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277809, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 388, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (403.52, 172.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671172 sequences (100000225 bp) in 1.892 CPU sec, 1.913 real sec...
[M::mem_process_seqs] Processed 670492 reads in 186.343 CPU sec, 6.036 real sec
[M::mem_process_seqs] Processed 670482 reads in 120.250 CPU sec, 3.891 real sec
sorting block 33, #reads = 1073839 mem = 536879330 peak = 1037168640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277967, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 390, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (405.96, 173.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672216 sequences (100000121 bp) in 2.740 CPU sec, 3.134 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278012, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 380, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (396.82, 171.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671836 sequences (100000220 bp) in 1.766 CPU sec, 1.784 real sec...
[M::mem_process_seqs] Processed 670606 reads in 183.428 CPU sec, 5.956 real sec
[M::mem_process_seqs] Processed 671172 reads in 118.049 CPU sec, 3.831 real sec
sorting block 34, #reads = 1072994 mem = 536880317 peak = 1037168640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278172, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 362, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (379.97, 163.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671458 sequences (100000184 bp) in 2.548 CPU sec, 2.996 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278897, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 366, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (383.21, 164.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672216 reads in 180.046 CPU sec, 5.862 real sec
[M::process] read 671326 sequences (100000138 bp) in 2.010 CPU sec, 2.033 real sec...
[M::mem_process_seqs] Processed 671836 reads in 114.045 CPU sec, 3.685 real sec
sorting block 35, #reads = 1074134 mem = 536878740 peak = 1037168640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 278388, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 369, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (385.83, 164.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1075852 mem = 536879137 peak = 1037168640
[M::process] read 671296 sequences (100000095 bp) in 2.594 CPU sec, 3.216 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278856, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 370, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (386.83, 164.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671458 reads in 176.325 CPU sec, 5.838 real sec
[M::process] read 671010 sequences (100000273 bp) in 1.906 CPU sec, 1.981 real sec...
[M::mem_process_seqs] Processed 671326 reads in 112.300 CPU sec, 3.756 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278640, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 373, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (388.75, 164.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 37, #reads = 1075346 mem = 536879900 peak = 1037168640
[M::process] read 670800 sequences (100000280 bp) in 2.514 CPU sec, 2.871 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278654, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 377, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (392.00, 165.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671296 reads in 179.038 CPU sec, 5.824 real sec
[M::process] read 670820 sequences (100000214 bp) in 1.904 CPU sec, 1.954 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278092, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 378, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (393.68, 166.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670800 reads in 100.693 CPU sec, 3.470 real sec
[M::process] read 670692 sequences (100000086 bp) in 2.120 CPU sec, 2.185 real sec...
sorting block 38, #reads = 1075106 mem = 536886739 peak = 1037168640
[M::mem_process_seqs] Processed 671010 reads in 257.942 CPU sec, 8.550 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278478, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 383, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (397.80, 167.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 39, #reads = 1075296 mem = 536879864 peak = 1037168640
[M::process] read 670440 sequences (100000165 bp) in 2.516 CPU sec, 2.938 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278534, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 387, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (401.61, 170.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670820 reads in 196.244 CPU sec, 6.425 real sec
[M::mem_process_seqs] Processed 670692 reads in 114.091 CPU sec, 3.736 real sec
[M::process] read 670622 sequences (100000056 bp) in 1.918 CPU sec, 2.155 real sec...
sorting block 40, #reads = 1075923 mem = 536880690 peak = 1037168640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278284, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 386, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (400.72, 169.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670306 sequences (100000176 bp) in 2.703 CPU sec, 2.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277994, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 384, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (400.33, 169.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670440 reads in 183.692 CPU sec, 5.918 real sec
[M::process] read 670486 sequences (100000296 bp) in 1.857 CPU sec, 1.865 real sec...
[M::mem_process_seqs] Processed 670622 reads in 114.882 CPU sec, 3.680 real sec
sorting block 41, #reads = 1075550 mem = 536879670 peak = 1037168640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278206, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 386, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (400.97, 168.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670366 sequences (100000193 bp) in 2.564 CPU sec, 2.939 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277726, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 385, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (400.52, 168.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670224 sequences (100000057 bp) in 1.822 CPU sec, 1.854 real sec...
[M::mem_process_seqs] Processed 670306 reads in 183.698 CPU sec, 5.930 real sec
[M::mem_process_seqs] Processed 670486 reads in 116.104 CPU sec, 3.708 real sec
sorting block 42, #reads = 1075140 mem = 536878730 peak = 1037168640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277852, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 386, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (401.27, 169.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670496 sequences (100000104 bp) in 2.766 CPU sec, 3.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277963, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 386, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (401.24, 168.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670500 sequences (100000137 bp) in 1.940 CPU sec, 1.953 real sec...
[M::mem_process_seqs] Processed 670366 reads in 184.153 CPU sec, 5.960 real sec
sorting block 43, #reads = 1075052 mem = 536878593 peak = 1037168640
[M::mem_process_seqs] Processed 670224 reads in 114.763 CPU sec, 3.665 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278038, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (396.70, 167.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 44, #reads = 1076134 mem = 536880609 peak = 1037168640
[M::process] read 670606 sequences (100000101 bp) in 2.555 CPU sec, 2.944 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278213, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 380, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (398.11, 169.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670626 sequences (100000140 bp) in 1.880 CPU sec, 1.901 real sec...
[M::mem_process_seqs] Processed 670496 reads in 184.316 CPU sec, 5.942 real sec
[M::mem_process_seqs] Processed 670500 reads in 116.615 CPU sec, 3.719 real sec
sorting block 45, #reads = 1075649 mem = 536878322 peak = 1037168640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277901, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 379, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (396.17, 168.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670622 sequences (100000063 bp) in 2.766 CPU sec, 3.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277780, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (395.92, 168.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670824 sequences (100000128 bp) in 1.947 CPU sec, 2.115 real sec...
[M::mem_process_seqs] Processed 670606 reads in 187.810 CPU sec, 6.044 real sec
[M::mem_process_seqs] Processed 670626 reads in 118.133 CPU sec, 3.775 real sec
sorting block 46, #reads = 1075106 mem = 536880666 peak = 1037168640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277983, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 376, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (393.67, 168.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670788 sequences (100000186 bp) in 2.419 CPU sec, 2.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277736, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 374, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (391.98, 167.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670662 sequences (100000043 bp) in 1.911 CPU sec, 1.915 real sec...
[M::mem_process_seqs] Processed 670622 reads in 188.364 CPU sec, 6.058 real sec
[M::mem_process_seqs] Processed 670824 reads in 119.032 CPU sec, 3.782 real sec
sorting block 47, #reads = 1074852 mem = 536881084 peak = 1037168640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277972, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 374, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (391.59, 166.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670598 sequences (100000189 bp) in 2.814 CPU sec, 3.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278251, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 373, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (390.60, 166.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670728 sequences (100000173 bp) in 1.927 CPU sec, 1.927 real sec...
[M::mem_process_seqs] Processed 670788 reads in 185.881 CPU sec, 5.976 real sec
sorting block 48, #reads = 1074030 mem = 536879656 peak = 1037168640
[M::mem_process_seqs] Processed 670662 reads in 117.347 CPU sec, 3.756 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277956, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 376, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (394.17, 168.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1074666 mem = 536877887 peak = 1037168640
[M::process] read 670474 sequences (100000214 bp) in 2.326 CPU sec, 2.641 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278520, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 379, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (397.20, 170.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (1403, 1403, 5927)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14975)
[M::mem_pestat] mean and std.dev: (2591.73, 2047.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19499)
[M::mem_pestat] skip orientation RR
[M::process] read 670462 sequences (100000198 bp) in 1.872 CPU sec, 1.880 real sec...
[M::mem_process_seqs] Processed 670598 reads in 183.044 CPU sec, 5.891 real sec
[M::mem_process_seqs] Processed 670728 reads in 114.902 CPU sec, 3.669 real sec
sorting block 50, #reads = 1074758 mem = 536882639 peak = 1037168640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 278080, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 386, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (402.46, 172.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670158 sequences (100000253 bp) in 2.778 CPU sec, 3.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278061, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 386, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (403.32, 173.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670962 sequences (100000173 bp) in 1.970 CPU sec, 1.973 real sec...
[M::mem_process_seqs] Processed 670474 reads in 184.996 CPU sec, 5.964 real sec
[M::mem_process_seqs] Processed 670462 reads in 116.096 CPU sec, 3.729 real sec
sorting block 51, #reads = 1074161 mem = 536877186 peak = 1037168640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278069, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 395, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (412.38, 178.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672164 sequences (100000105 bp) in 2.448 CPU sec, 2.778 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278418, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 384, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (401.92, 174.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671632 sequences (100000154 bp) in 1.835 CPU sec, 1.865 real sec...
[M::mem_process_seqs] Processed 670158 reads in 182.647 CPU sec, 5.895 real sec
[M::mem_process_seqs] Processed 670962 reads in 118.045 CPU sec, 3.780 real sec
sorting block 52, #reads = 1073959 mem = 536879465 peak = 1037168640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 278001, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 366, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (384.90, 167.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671264 sequences (100000165 bp) in 2.845 CPU sec, 3.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277442, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 370, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (388.19, 167.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671048 sequences (100000055 bp) in 1.796 CPU sec, 1.803 real sec...
[M::mem_process_seqs] Processed 672164 reads in 185.143 CPU sec, 5.939 real sec
sorting block 53, #reads = 1074111 mem = 536876536 peak = 1037168640
[M::mem_process_seqs] Processed 671632 reads in 115.049 CPU sec, 3.673 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277506, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 379, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (397.01, 171.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 54, #reads = 1077536 mem = 536879943 peak = 1066618880
[M::process] read 671166 sequences (100000140 bp) in 2.479 CPU sec, 2.844 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277586, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 378, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (394.90, 169.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670882 sequences (100000071 bp) in 1.797 CPU sec, 1.813 real sec...
[M::mem_process_seqs] Processed 671264 reads in 184.979 CPU sec, 5.990 real sec
[M::mem_process_seqs] Processed 671048 reads in 115.579 CPU sec, 3.713 real sec
sorting block 55, #reads = 1076830 mem = 536878339 peak = 1066618880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278044, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 376, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (392.61, 166.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670764 sequences (100000065 bp) in 2.617 CPU sec, 2.985 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277322, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (395.74, 168.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670620 sequences (100000301 bp) in 1.954 CPU sec, 2.081 real sec...
[M::mem_process_seqs] Processed 671166 reads in 183.134 CPU sec, 5.920 real sec
[M::mem_process_seqs] Processed 670882 reads in 116.259 CPU sec, 3.740 real sec
sorting block 56, #reads = 1077093 mem = 536883691 peak = 1066618880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277499, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 385, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (400.80, 170.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670458 sequences (100000274 bp) in 2.453 CPU sec, 2.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277114, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 387, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (402.52, 170.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670288 sequences (100000087 bp) in 1.761 CPU sec, 1.840 real sec...
[M::mem_process_seqs] Processed 670764 reads in 187.512 CPU sec, 6.065 real sec
sorting block 57, #reads = 1076964 mem = 536882096 peak = 1066618880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277117, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 389, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (404.65, 171.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670428 sequences (100000088 bp) in 2.154 CPU sec, 2.223 real sec...
[M::mem_process_seqs] Processed 670458 reads in 108.539 CPU sec, 3.682 real sec
[M::mem_process_seqs] Processed 670620 reads in 276.766 CPU sec, 9.124 real sec
sorting block 58, #reads = 1075922 mem = 536881667 peak = 1066618880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277103, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 392, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (406.19, 171.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670398 sequences (100000181 bp) in 2.515 CPU sec, 3.019 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277296, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 389, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (403.94, 170.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670288 reads in 204.321 CPU sec, 6.618 real sec
[M::mem_process_seqs] Processed 670428 reads in 116.889 CPU sec, 3.739 real sec
[M::process] read 670484 sequences (100000078 bp) in 2.129 CPU sec, 2.200 real sec...
sorting block 59, #reads = 1075738 mem = 536880030 peak = 1066618880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277213, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 388, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (403.30, 170.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670478 sequences (100000076 bp) in 2.152 CPU sec, 2.517 real sec...
[M::process] read 670106 sequences (100000179 bp) in 1.906 CPU sec, 1.924 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277306, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 387, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (401.94, 169.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670398 reads in 191.523 CPU sec, 6.189 real sec
sorting block 60, #reads = 1076427 mem = 536876721 peak = 1066618880
[M::mem_process_seqs] Processed 670484 reads in 119.482 CPU sec, 3.839 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277538, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 385, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (399.62, 167.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 61, #reads = 1074519 mem = 536877772 peak = 1066618880
[M::process] read 670208 sequences (100000139 bp) in 2.365 CPU sec, 2.805 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277408, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 386, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (401.41, 167.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670808 sequences (100000202 bp) in 1.940 CPU sec, 1.948 real sec...
[M::mem_process_seqs] Processed 670478 reads in 190.484 CPU sec, 6.118 real sec
[M::mem_process_seqs] Processed 670106 reads in 120.474 CPU sec, 3.826 real sec
sorting block 62, #reads = 1075275 mem = 536878071 peak = 1066618880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277819, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 388, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (403.16, 169.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671078 sequences (100000221 bp) in 2.240 CPU sec, 2.823 real sec...
[M::process] read 670760 sequences (100000123 bp) in 1.796 CPU sec, 1.806 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277456, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 375, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (391.71, 166.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670208 reads in 192.369 CPU sec, 6.227 real sec
[M::mem_process_seqs] Processed 670808 reads in 122.041 CPU sec, 3.920 real sec
sorting block 63, #reads = 1075092 mem = 536877916 peak = 1066618880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277762, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 370, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (388.08, 165.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670722 sequences (100000119 bp) in 2.453 CPU sec, 2.709 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278053, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (393.04, 166.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670886 sequences (100000102 bp) in 1.734 CPU sec, 1.784 real sec...
[M::mem_process_seqs] Processed 671078 reads in 190.140 CPU sec, 6.121 real sec
[M::mem_process_seqs] Processed 670760 reads in 119.195 CPU sec, 3.823 real sec
sorting block 64, #reads = 1075376 mem = 536880141 peak = 1066618880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277977, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 376, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (391.90, 165.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670878 sequences (100000012 bp) in 2.566 CPU sec, 2.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277815, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 373, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (389.75, 165.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670926 sequences (100000144 bp) in 1.804 CPU sec, 1.846 real sec...
[M::mem_process_seqs] Processed 670722 reads in 189.919 CPU sec, 6.139 real sec
sorting block 65, #reads = 1075366 mem = 536884388 peak = 1066618880
[M::mem_process_seqs] Processed 670886 reads in 119.858 CPU sec, 3.843 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278039, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 370, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (387.76, 164.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 66, #reads = 1075560 mem = 536878104 peak = 1066618880
[M::process] read 670620 sequences (100000130 bp) in 2.425 CPU sec, 2.621 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277313, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 670720 sequences (100000157 bp) in 1.939 CPU sec, 1.970 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (264, 373, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (390.04, 165.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670878 reads in 187.008 CPU sec, 6.012 real sec
[M::mem_process_seqs] Processed 670926 reads in 119.827 CPU sec, 3.840 real sec
sorting block 67, #reads = 1075340 mem = 536880167 peak = 1066618880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277173, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (394.96, 167.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670586 sequences (100000154 bp) in 2.487 CPU sec, 2.698 real sec...
[M::process] read 669956 sequences (100000143 bp) in 1.813 CPU sec, 1.849 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277718, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (393.39, 166.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670620 reads in 187.162 CPU sec, 6.042 real sec
[M::mem_process_seqs] Processed 670720 reads in 117.416 CPU sec, 3.780 real sec
sorting block 68, #reads = 1074602 mem = 536878278 peak = 1066618880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277860, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (399.09, 169.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670510 sequences (100000062 bp) in 2.531 CPU sec, 2.758 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 278260, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 391, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (406.97, 171.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670500 sequences (100000119 bp) in 1.871 CPU sec, 1.889 real sec...
[M::mem_process_seqs] Processed 670586 reads in 183.149 CPU sec, 5.853 real sec
[M::mem_process_seqs] Processed 669956 reads in 116.515 CPU sec, 3.698 real sec
sorting block 69, #reads = 1074236 mem = 536877910 peak = 1066618880
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278200, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 386, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (402.98, 172.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674400 sequences (100000208 bp) in 2.682 CPU sec, 2.975 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278458, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 389, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (405.49, 174.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670510 reads in 183.542 CPU sec, 5.901 real sec
[M::process] read 673766 sequences (100000271 bp) in 2.087 CPU sec, 2.092 real sec...
sorting block 70, #reads = 1075046 mem = 536884745 peak = 1066618880
[M::mem_process_seqs] Processed 670500 reads in 118.466 CPU sec, 3.780 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279919, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 344, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (365.71, 163.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 71, #reads = 1074826 mem = 536885510 peak = 1090691072
[M::process] read 673162 sequences (100000087 bp) in 2.616 CPU sec, 2.762 real sec...
[M::process] read 672730 sequences (100000151 bp) in 2.116 CPU sec, 2.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279703, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 355, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (372.20, 163.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674400 reads in 204.312 CPU sec, 6.535 real sec
[M::mem_process_seqs] Processed 673766 reads in 129.126 CPU sec, 4.108 real sec
sorting block 72, #reads = 1071864 mem = 536876698 peak = 1090691072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 279552, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 360, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (376.54, 163.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672296 sequences (100000084 bp) in 2.551 CPU sec, 2.803 real sec...
[M::process] read 671794 sequences (100000061 bp) in 1.872 CPU sec, 1.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 279155, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 367, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (382.19, 166.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673162 reads in 201.351 CPU sec, 6.459 real sec
[M::mem_process_seqs] Processed 672730 reads in 125.818 CPU sec, 4.020 real sec
sorting block 73, #reads = 1072109 mem = 536878291 peak = 1090691072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279262, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 370, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (384.42, 165.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671472 sequences (100000005 bp) in 2.436 CPU sec, 2.776 real sec...
[M::process] read 671242 sequences (100000107 bp) in 2.022 CPU sec, 2.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278239, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 379, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (391.89, 167.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672296 reads in 199.256 CPU sec, 6.401 real sec
[M::mem_process_seqs] Processed 671794 reads in 127.752 CPU sec, 4.075 real sec
sorting block 74, #reads = 1072288 mem = 536882025 peak = 1090691072
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278423, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 385, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (396.98, 168.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671424 sequences (100000098 bp) in 2.451 CPU sec, 2.805 real sec...
[M::process] read 671516 sequences (100000214 bp) in 2.025 CPU sec, 2.091 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277777, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 387, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (398.93, 169.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671472 reads in 197.028 CPU sec, 6.332 real sec
sorting block 75, #reads = 1072931 mem = 536886810 peak = 1090691072
[M::mem_process_seqs] Processed 671242 reads in 127.711 CPU sec, 4.080 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277880, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (397.26, 168.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 76, #reads = 1072879 mem = 536879366 peak = 1096372224
[M::process] read 671480 sequences (100000156 bp) in 2.633 CPU sec, 2.924 real sec...
[M::process] read 671402 sequences (100000098 bp) in 1.975 CPU sec, 1.978 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277895, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 382, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (393.68, 165.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671424 reads in 201.071 CPU sec, 6.451 real sec
[M::mem_process_seqs] Processed 671516 reads in 131.009 CPU sec, 4.169 real sec
sorting block 77, #reads = 1071471 mem = 536881105 peak = 1096372224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278712, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 383, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (393.98, 165.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671262 sequences (100000211 bp) in 2.625 CPU sec, 2.896 real sec...
[M::process] read 671588 sequences (100000131 bp) in 1.938 CPU sec, 1.948 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278705, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 383, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (394.33, 165.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671480 reads in 204.415 CPU sec, 6.556 real sec
[M::mem_process_seqs] Processed 671402 reads in 128.233 CPU sec, 4.096 real sec
sorting block 78, #reads = 1068582 mem = 536876495 peak = 1096372224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278525, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 383, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (394.35, 165.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671200 sequences (100000260 bp) in 2.606 CPU sec, 2.918 real sec...
[M::process] read 671168 sequences (100000111 bp) in 2.005 CPU sec, 2.012 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278272, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (391.88, 166.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671262 reads in 201.161 CPU sec, 6.460 real sec
[M::mem_process_seqs] Processed 671588 reads in 128.343 CPU sec, 4.090 real sec
sorting block 79, #reads = 1070557 mem = 536881226 peak = 1096372224
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278286, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (394.65, 166.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671092 sequences (100000292 bp) in 2.612 CPU sec, 2.902 real sec...
[M::process] read 670720 sequences (100000118 bp) in 2.008 CPU sec, 2.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278288, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (393.42, 163.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671200 reads in 204.941 CPU sec, 6.583 real sec
sorting block 80, #reads = 1071329 mem = 536879515 peak = 1096372224
[M::process] read 671080 sequences (100000045 bp) in 2.184 CPU sec, 2.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278077, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 385, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (395.42, 165.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671092 reads in 119.773 CPU sec, 4.015 real sec
sorting block 81, #reads = 1069760 mem = 536882155 peak = 1096372224
[M::mem_process_seqs] Processed 671168 reads in 288.753 CPU sec, 9.426 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277828, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 391, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (401.20, 167.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670918 sequences (100000000 bp) in 2.935 CPU sec, 3.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278497, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 385, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (396.42, 166.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670720 reads in 231.185 CPU sec, 7.421 real sec
[M::process] read 670644 sequences (100000168 bp) in 2.342 CPU sec, 2.390 real sec...
sorting block 82, #reads = 1070339 mem = 536881001 peak = 1096372224
[M::mem_process_seqs] Processed 671080 reads in 136.443 CPU sec, 4.327 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 278068, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (999, 999, 999)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (999, 999)
[M::mem_pestat] mean and std.dev: (999.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (999, 999)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 383, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (396.18, 166.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 670576 sequences (100000241 bp) in 2.687 CPU sec, 3.009 real sec...
sorting block 83, #reads = 1068963 mem = 536878395 peak = 1108525056
[M::process] read 670692 sequences (100000078 bp) in 1.823 CPU sec, 1.833 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277589, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 385, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (397.26, 165.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670918 reads in 210.598 CPU sec, 6.791 real sec
[M::mem_process_seqs] Processed 670644 reads in 133.598 CPU sec, 4.287 real sec
sorting block 84, #reads = 1069205 mem = 536876662 peak = 1108525056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278179, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 389, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (401.07, 167.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670544 sequences (100000059 bp) in 2.702 CPU sec, 2.930 real sec...
[M::process] read 670340 sequences (100000014 bp) in 1.958 CPU sec, 1.971 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 278509, 2, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (275, 275, 1461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3833)
[M::mem_pestat] mean and std.dev: (883.17, 801.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5019)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 388, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (400.85, 168.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 670576 reads in 206.971 CPU sec, 6.642 real sec
[M::mem_process_seqs] Processed 670692 reads in 131.933 CPU sec, 4.203 real sec
sorting block 85, #reads = 1070224 mem = 536880912 peak = 1108525056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279160, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 395, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (407.76, 172.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673876 sequences (100000137 bp) in 3.048 CPU sec, 3.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278621, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 395, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (407.94, 172.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675104 sequences (100000037 bp) in 1.947 CPU sec, 2.084 real sec...
[M::mem_process_seqs] Processed 670544 reads in 200.193 CPU sec, 6.433 real sec
[M::mem_process_seqs] Processed 670340 reads in 126.657 CPU sec, 4.055 real sec
sorting block 86, #reads = 1070444 mem = 536878354 peak = 1108525056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279459, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (371.76, 165.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674232 sequences (100000192 bp) in 2.596 CPU sec, 2.964 real sec...
[M::process] read 674060 sequences (100000144 bp) in 1.905 CPU sec, 1.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280487, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 337, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (354.66, 155.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673876 reads in 214.918 CPU sec, 6.919 real sec
sorting block 87, #reads = 1070207 mem = 536884747 peak = 1108525056
[M::mem_process_seqs] Processed 675104 reads in 136.829 CPU sec, 4.363 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280385, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (361.97, 156.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 88, #reads = 1066721 mem = 536881423 peak = 1108525056
[M::process] read 673886 sequences (100000237 bp) in 2.807 CPU sec, 3.151 real sec...
[M::process] read 673486 sequences (100000194 bp) in 1.921 CPU sec, 2.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280192, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (364.02, 156.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674232 reads in 210.756 CPU sec, 6.806 real sec
[M::mem_process_seqs] Processed 674060 reads in 130.996 CPU sec, 4.211 real sec
sorting block 89, #reads = 1068636 mem = 536880631 peak = 1108525056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280494, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (361.09, 153.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673130 sequences (100000092 bp) in 2.626 CPU sec, 3.014 real sec...
[M::process] read 672674 sequences (100000033 bp) in 1.994 CPU sec, 2.003 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279699, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 354, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (367.52, 156.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673886 reads in 213.837 CPU sec, 6.870 real sec
[M::mem_process_seqs] Processed 673486 reads in 134.030 CPU sec, 4.303 real sec
sorting block 90, #reads = 1067188 mem = 536879343 peak = 1108525056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279536, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 356, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (369.40, 156.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672776 sequences (100000158 bp) in 2.899 CPU sec, 3.164 real sec...
[M::process] read 672288 sequences (100000048 bp) in 1.926 CPU sec, 1.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279871, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 364, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (376.94, 159.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673130 reads in 204.539 CPU sec, 6.570 real sec
[M::mem_process_seqs] Processed 672674 reads in 126.703 CPU sec, 4.057 real sec
sorting block 91, #reads = 1066288 mem = 536878903 peak = 1108525056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279803, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 365, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (378.33, 161.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672104 sequences (100000081 bp) in 2.582 CPU sec, 2.908 real sec...
[M::process] read 672112 sequences (100000021 bp) in 1.870 CPU sec, 1.880 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279303, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 367, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (379.43, 160.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672776 reads in 205.424 CPU sec, 6.612 real sec
sorting block 92, #reads = 1069336 mem = 536877201 peak = 1108525056
[M::mem_process_seqs] Processed 672288 reads in 127.134 CPU sec, 4.065 real sec
sorting block 93, #reads = 1068266 mem = 536877748 peak = 1108525056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279331, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 370, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (382.27, 160.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672376 sequences (100000300 bp) in 2.972 CPU sec, 3.276 real sec...
[M::process] read 671684 sequences (100000270 bp) in 1.856 CPU sec, 1.890 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279770, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 370, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (382.15, 161.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672104 reads in 206.375 CPU sec, 6.624 real sec
[M::mem_process_seqs] Processed 672112 reads in 130.838 CPU sec, 4.167 real sec
sorting block 94, #reads = 1069068 mem = 536882914 peak = 1108525056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279317, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 367, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (381.31, 162.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671520 sequences (100000133 bp) in 2.516 CPU sec, 2.793 real sec...
[M::process] read 671642 sequences (100000295 bp) in 1.822 CPU sec, 1.843 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278953, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (390.54, 164.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672376 reads in 206.107 CPU sec, 6.619 real sec
[M::mem_process_seqs] Processed 671684 reads in 128.917 CPU sec, 4.128 real sec
sorting block 95, #reads = 1068449 mem = 536878540 peak = 1108525056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278499, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 379, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (392.64, 167.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670954 sequences (100000223 bp) in 2.679 CPU sec, 3.054 real sec...
[M::process] read 670668 sequences (100000261 bp) in 1.915 CPU sec, 1.936 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278620, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 378, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (393.46, 170.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671520 reads in 203.057 CPU sec, 6.526 real sec
sorting block 96, #reads = 1070034 mem = 536883061 peak = 1108525056
[M::mem_process_seqs] Processed 671642 reads in 129.413 CPU sec, 4.135 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 278564, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 391, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (403.62, 171.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670960 sequences (100000075 bp) in 2.505 CPU sec, 3.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278700, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 393, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (406.40, 174.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1312)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670828 sequences (100000272 bp) in 1.989 CPU sec, 2.042 real sec...
[M::mem_process_seqs] Processed 670954 reads in 200.801 CPU sec, 6.469 real sec
sorting block 97, #reads = 1070347 mem = 536877880 peak = 1108525056
[M::mem_process_seqs] Processed 670668 reads in 126.626 CPU sec, 4.045 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279237, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 396, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069)
[M::mem_pestat] mean and std.dev: (409.10, 176.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1336)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 98, #reads = 1071001 mem = 536878656 peak = 1108525056
[M::process] read 670508 sequences (100000044 bp) in 2.800 CPU sec, 3.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278288, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 394, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056)
[M::mem_pestat] mean and std.dev: (408.25, 175.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1318)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670462 sequences (100000203 bp) in 1.903 CPU sec, 1.911 real sec...
[M::mem_process_seqs] Processed 670960 reads in 197.130 CPU sec, 6.350 real sec
[M::mem_process_seqs] Processed 670828 reads in 123.708 CPU sec, 3.949 real sec
sorting block 99, #reads = 1071569 mem = 536878809 peak = 1108525056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279048, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 395, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (409.54, 175.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1312)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670470 sequences (100000292 bp) in 2.538 CPU sec, 2.953 real sec...
[M::process] read 672088 sequences (100000091 bp) in 1.990 CPU sec, 1.997 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278612, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 401, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071)
[M::mem_pestat] mean and std.dev: (414.05, 177.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1337)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670508 reads in 202.169 CPU sec, 6.535 real sec
[M::mem_process_seqs] Processed 670462 reads in 127.601 CPU sec, 4.103 real sec
sorting block 100, #reads = 1070251 mem = 536877888 peak = 1108525056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278786, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 397, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (410.81, 176.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1316)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673630 sequences (100000047 bp) in 3.094 CPU sec, 3.386 real sec...
[M::process] read 672790 sequences (100000115 bp) in 1.891 CPU sec, 1.910 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279629, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 375, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (391.88, 172.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670470 reads in 214.689 CPU sec, 6.884 real sec
sorting block 101, #reads = 1068176 mem = 536879176 peak = 1108525056
[M::mem_process_seqs] Processed 672088 reads in 137.823 CPU sec, 4.408 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279826, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 355, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (375.19, 167.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 102, #reads = 1063132 mem = 536880313 peak = 1108525056
[M::process] read 672782 sequences (100000121 bp) in 2.635 CPU sec, 2.863 real sec...
[M::process] read 672268 sequences (100000182 bp) in 1.929 CPU sec, 1.949 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280032, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 362, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (379.14, 166.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673630 reads in 197.303 CPU sec, 6.368 real sec
[M::mem_process_seqs] Processed 672790 reads in 119.542 CPU sec, 3.852 real sec
sorting block 103, #reads = 1072131 mem = 536878999 peak = 1108525056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279382, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 363, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (378.93, 163.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671888 sequences (100000199 bp) in 2.612 CPU sec, 2.901 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279200, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 368, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (384.23, 165.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671726 sequences (100000220 bp) in 1.918 CPU sec, 1.930 real sec...
[M::mem_process_seqs] Processed 672782 reads in 193.856 CPU sec, 6.235 real sec
[M::mem_process_seqs] Processed 672268 reads in 121.030 CPU sec, 3.886 real sec
sorting block 104, #reads = 1073273 mem = 536878049 peak = 1108525056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279140, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 372, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (387.55, 167.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671656 sequences (100000074 bp) in 2.727 CPU sec, 3.042 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279677, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 378, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (393.00, 170.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671406 sequences (100000017 bp) in 1.950 CPU sec, 1.961 real sec...
[M::mem_process_seqs] Processed 671888 reads in 190.179 CPU sec, 6.141 real sec
[M::mem_process_seqs] Processed 671726 reads in 121.657 CPU sec, 3.901 real sec
sorting block 105, #reads = 1072965 mem = 536877548 peak = 1108525056
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279243, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 380, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (394.35, 170.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671598 sequences (100000072 bp) in 2.634 CPU sec, 2.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278926, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (396.04, 170.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671616 sequences (100000039 bp) in 1.950 CPU sec, 1.968 real sec...
[M::mem_process_seqs] Processed 671656 reads in 187.762 CPU sec, 6.040 real sec
sorting block 106, #reads = 1073263 mem = 536882735 peak = 1108525056
[M::mem_process_seqs] Processed 671406 reads in 120.886 CPU sec, 3.845 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278917, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (393.18, 168.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 107, #reads = 1073588 mem = 536878516 peak = 1109590016
[M::process] read 671788 sequences (100000029 bp) in 2.569 CPU sec, 2.978 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279198, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (391.76, 167.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671154 sequences (100000010 bp) in 1.991 CPU sec, 1.998 real sec...
[M::mem_process_seqs] Processed 671598 reads in 191.741 CPU sec, 6.203 real sec
[M::mem_process_seqs] Processed 671616 reads in 125.502 CPU sec, 4.018 real sec
sorting block 108, #reads = 1073157 mem = 536880450 peak = 1109590016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279016, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (391.29, 168.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671118 sequences (100000162 bp) in 2.708 CPU sec, 3.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278968, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 383, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (397.50, 169.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671040 sequences (100000287 bp) in 2.010 CPU sec, 2.025 real sec...
[M::mem_process_seqs] Processed 671788 reads in 196.185 CPU sec, 6.321 real sec
[M::mem_process_seqs] Processed 671154 reads in 126.986 CPU sec, 4.067 real sec
sorting block 109, #reads = 1072061 mem = 536881974 peak = 1109590016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278945, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (394.58, 166.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670962 sequences (100000028 bp) in 2.481 CPU sec, 2.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278446, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 387, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (400.88, 171.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670854 sequences (100000214 bp) in 1.891 CPU sec, 1.933 real sec...
[M::mem_process_seqs] Processed 671118 reads in 191.518 CPU sec, 6.173 real sec
[M::mem_process_seqs] Processed 671040 reads in 124.078 CPU sec, 3.957 real sec
sorting block 110, #reads = 1071596 mem = 536878916 peak = 1109590016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277877, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 388, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (402.55, 172.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670726 sequences (100000301 bp) in 2.915 CPU sec, 3.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278713, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 670880 sequences (100000020 bp) in 1.784 CPU sec, 1.827 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (269, 390, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (405.02, 173.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670962 reads in 198.491 CPU sec, 6.389 real sec
sorting block 111, #reads = 1071659 mem = 536877692 peak = 1109590016
[M::mem_process_seqs] Processed 670854 reads in 125.273 CPU sec, 4.005 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278958, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 387, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (402.00, 171.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 112, #reads = 1071803 mem = 536882290 peak = 1109590016
[M::process] read 670712 sequences (100000254 bp) in 2.466 CPU sec, 2.916 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278970, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 390, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (404.12, 173.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670756 sequences (100000238 bp) in 2.079 CPU sec, 2.108 real sec...
[M::mem_process_seqs] Processed 670726 reads in 197.589 CPU sec, 6.370 real sec
[M::mem_process_seqs] Processed 670880 reads in 124.603 CPU sec, 3.973 real sec
sorting block 113, #reads = 1071662 mem = 536883486 peak = 1109590016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278246, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 387, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (401.80, 171.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670574 sequences (100000164 bp) in 2.916 CPU sec, 3.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278689, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 388, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (402.81, 172.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670770 sequences (100000237 bp) in 1.785 CPU sec, 1.870 real sec...
[M::mem_process_seqs] Processed 670712 reads in 199.056 CPU sec, 6.407 real sec
[M::mem_process_seqs] Processed 670756 reads in 126.657 CPU sec, 4.058 real sec
sorting block 114, #reads = 1071720 mem = 536881587 peak = 1109590016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278615, 7, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 389, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (404.39, 173.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (3119, 5336, 5485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10217)
[M::mem_pestat] mean and std.dev: (4550.25, 1262.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12583)
[M::mem_pestat] skip orientation RR
[M::process] read 671056 sequences (100000285 bp) in 2.806 CPU sec, 3.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278585, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 388, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (403.20, 173.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670854 sequences (100000238 bp) in 1.999 CPU sec, 2.006 real sec...
[M::mem_process_seqs] Processed 670574 reads in 198.160 CPU sec, 6.369 real sec
[M::mem_process_seqs] Processed 670770 reads in 128.406 CPU sec, 4.110 real sec
sorting block 115, #reads = 1071026 mem = 536878474 peak = 1109590016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278814, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 387, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (403.54, 175.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670556 sequences (100000130 bp) in 2.905 CPU sec, 3.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278818, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 389, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (405.33, 176.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671092 sequences (100000242 bp) in 1.874 CPU sec, 1.932 real sec...
[M::mem_process_seqs] Processed 671056 reads in 200.928 CPU sec, 6.496 real sec
sorting block 116, #reads = 1070864 mem = 536878953 peak = 1109590016
[M::mem_process_seqs] Processed 670854 reads in 131.448 CPU sec, 4.193 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278893, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 391, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (408.49, 178.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 117, #reads = 1069899 mem = 536879839 peak = 1109590016
[M::process] read 676298 sequences (100000174 bp) in 2.997 CPU sec, 3.490 real sec...
[M::process] read 674884 sequences (100000131 bp) in 2.024 CPU sec, 2.032 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278139, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 388, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057)
[M::mem_pestat] mean and std.dev: (407.00, 181.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670556 reads in 213.049 CPU sec, 6.851 real sec
[M::mem_process_seqs] Processed 671092 reads in 144.966 CPU sec, 4.629 real sec
sorting block 118, #reads = 1067431 mem = 536878681 peak = 1109590016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281104, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (347.81, 154.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674114 sequences (100000026 bp) in 2.959 CPU sec, 3.412 real sec...
[M::process] read 673436 sequences (100000016 bp) in 2.016 CPU sec, 2.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280447, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (357.48, 156.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676298 reads in 216.576 CPU sec, 6.954 real sec
[M::mem_process_seqs] Processed 674884 reads in 139.528 CPU sec, 4.463 real sec
sorting block 119, #reads = 1063035 mem = 536877765 peak = 1109590016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280213, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 346, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (361.95, 155.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673268 sequences (100000066 bp) in 2.672 CPU sec, 3.024 real sec...
[M::process] read 672810 sequences (100000118 bp) in 1.995 CPU sec, 2.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279733, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 351, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (364.51, 154.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674114 reads in 210.406 CPU sec, 6.768 real sec
[M::mem_process_seqs] Processed 673436 reads in 134.213 CPU sec, 4.284 real sec
sorting block 120, #reads = 1067817 mem = 536880443 peak = 1109590016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279765, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 359, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (372.18, 158.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672524 sequences (100000187 bp) in 2.593 CPU sec, 2.996 real sec...
[M::process] read 672278 sequences (100000106 bp) in 1.872 CPU sec, 1.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279537, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 363, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (377.10, 161.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673268 reads in 218.107 CPU sec, 7.040 real sec
sorting block 121, #reads = 1067997 mem = 536878327 peak = 1109590016
[M::mem_process_seqs] Processed 672810 reads in 136.728 CPU sec, 4.385 real sec
sorting block 122, #reads = 1066581 mem = 536877464 peak = 1152929792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279495, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 368, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (380.99, 162.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672358 sequences (100000205 bp) in 2.613 CPU sec, 2.876 real sec...
[M::process] read 672124 sequences (100000112 bp) in 2.010 CPU sec, 2.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278818, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 371, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (384.26, 163.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672524 reads in 208.671 CPU sec, 6.724 real sec
[M::mem_process_seqs] Processed 672278 reads in 137.528 CPU sec, 4.403 real sec
sorting block 123, #reads = 1069957 mem = 536879246 peak = 1152929792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279598, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 366, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (379.42, 160.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671452 sequences (100000182 bp) in 2.760 CPU sec, 3.252 real sec...
[M::process] read 671072 sequences (100000070 bp) in 1.969 CPU sec, 2.018 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279426, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 365, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (378.69, 159.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672358 reads in 211.815 CPU sec, 6.829 real sec
[M::mem_process_seqs] Processed 672124 reads in 138.171 CPU sec, 4.425 real sec
sorting block 124, #reads = 1068204 mem = 536880623 peak = 1152929792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279163, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 377, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (389.18, 163.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671766 sequences (100000299 bp) in 2.897 CPU sec, 3.282 real sec...
[M::process] read 671540 sequences (100000267 bp) in 2.098 CPU sec, 2.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279354, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 380, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (391.75, 163.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671452 reads in 209.703 CPU sec, 6.727 real sec
sorting block 125, #reads = 1067880 mem = 536880840 peak = 1152929792
[M::mem_process_seqs] Processed 671072 reads in 135.911 CPU sec, 4.338 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279134, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 371, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (384.30, 161.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 126, #reads = 1068785 mem = 536876991 peak = 1152929792
[M::process] read 671340 sequences (100000168 bp) in 2.964 CPU sec, 3.422 real sec...
[M::process] read 671406 sequences (100000261 bp) in 1.989 CPU sec, 1.996 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278469, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 373, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (386.30, 162.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671766 reads in 210.290 CPU sec, 6.759 real sec
[M::mem_process_seqs] Processed 671540 reads in 134.108 CPU sec, 4.282 real sec
sorting block 127, #reads = 1069458 mem = 536879886 peak = 1152929792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 278490, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (389.07, 163.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (1023, 1023, 5657)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14925)
[M::mem_pestat] mean and std.dev: (2722.09, 2264.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19559)
[M::mem_pestat] skip orientation RR
[M::process] read 671190 sequences (100000008 bp) in 2.986 CPU sec, 3.256 real sec...
[M::process] read 671260 sequences (100000252 bp) in 2.001 CPU sec, 2.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278586, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (390.60, 164.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671340 reads in 206.335 CPU sec, 6.630 real sec
[M::mem_process_seqs] Processed 671406 reads in 130.377 CPU sec, 4.177 real sec
sorting block 128, #reads = 1070236 mem = 536877131 peak = 1152929792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278564, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 376, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (390.72, 165.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671100 sequences (100000228 bp) in 2.579 CPU sec, 3.156 real sec...
[M::process] read 671104 sequences (100000134 bp) in 1.635 CPU sec, 1.648 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278447, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (395.05, 167.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671190 reads in 203.464 CPU sec, 6.560 real sec
[M::mem_process_seqs] Processed 671260 reads in 129.228 CPU sec, 4.131 real sec
sorting block 129, #reads = 1071039 mem = 536880902 peak = 1152929792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278011, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 383, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (396.97, 169.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670812 sequences (100000131 bp) in 2.868 CPU sec, 3.121 real sec...
[M::process] read 670024 sequences (100000078 bp) in 1.817 CPU sec, 1.883 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278287, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 383, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (398.18, 170.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671100 reads in 205.887 CPU sec, 6.616 real sec
sorting block 130, #reads = 1071063 mem = 536878712 peak = 1152929792
[M::mem_process_seqs] Processed 671104 reads in 129.390 CPU sec, 4.137 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278786, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 387, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (401.73, 172.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670734 sequences (100000163 bp) in 2.721 CPU sec, 3.048 real sec...
sorting block 131, #reads = 1071253 mem = 536878479 peak = 1152929792
[M::process] read 510978 sequences (76153788 bp) in 1.474 CPU sec, 1.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277620, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 402, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (414.80, 175.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670812 reads in 204.947 CPU sec, 6.627 real sec
[M::mem_process_seqs] Processed 670024 reads in 129.172 CPU sec, 4.149 real sec
sorting block 132, #reads = 1069955 mem = 536879615 peak = 1152929792
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 212348, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 386, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (402.20, 173.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 510978 reads in 84.691 CPU sec, 2.783 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278240, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 388, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (404.62, 174.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670734 reads in 191.369 CPU sec, 6.891 real sec
sorting block 133, #reads = 1069911 mem = 536877482 peak = 1152929792
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 143491474
overall: 69785337856 mem 24400.691 user 487.285 sys 829.890 real
sorting block 134, #reads = 450142 mem = 225669864 peak = 1152929792
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_Kh-T1-TDNA-1_A23MHWWLT4_1.L007\tPU:L007\tSM:659_Kh-T1-TDNA-1_A23MHWWLT4_1\tLB:659_Kh-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_Kh-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_Kh-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 829.993 sec; CPU: 24888.048 sec
uniq header count 1
merging 135 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8315780698
output reads: 144190997
bam_mem_sort: 135 calls 94.582 user 1.828 sys 100.889 real
bam_write: 135 calls 85.315 user 5.172 sys 184.083 real
execute: 1 calls 46.712 user 3.452 sys 894.037 real
merge_files: 1 calls 46.680 user 3.433 sys 63.646 real
parse_chunk: 2151 calls 99.766 user 27.697 sys 171.736 real
read_chunk: 5794 calls 54.634 user 32.508 sys 778.553 real
sort_block: 1 calls 33.824 user 31.513 sys 747.203 real
write_chunk: 3641 calls 46.158 user 3.083 sys 62.146 real
overall: 3152281600 mem 2357.925 user 94.082 sys 894.057 real
ls: cannot access '*.cram': No such file or directory