.command.err - Workdir - DAQ:SENTIEON_BWAMEM (659_d0M-T1-TDNA-1_A23MHWWLT4_1;L008;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/3a/db05cbe6ead0a5c176560cb9230f7a/.command.err
Size: 206.3 KB
Task: DAQ:SENTIEON_BWAMEM (659_d0M-T1-TDNA-1_A23MHWWLT4_1;L008;0001)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_d0M-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1933.616 CPU sec, 77.738 real sec
[M::process] read 676564 sequences (100000004 bp) in 1.286 CPU sec, 1.287 real sec...
[M::process] read 673610 sequences (100000122 bp) in 1.600 CPU sec, 1.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284908, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (347.54, 159.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674218 sequences (100000103 bp) in 1.580 CPU sec, 1.575 real sec...
[M::process] read 674796 sequences (100000035 bp) in 1.530 CPU sec, 1.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284163, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 354, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (369.62, 163.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676564 reads in 165.648 CPU sec, 5.216 real sec
[M::mem_process_seqs] Processed 673610 reads in 88.650 CPU sec, 2.810 real sec
sorting block 0, #reads = 1080517 mem = 536878021 peak = 583733248
[M::process] read 675906 sequences (100000240 bp) in 1.672 CPU sec, 1.760 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284306, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 350, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (366.81, 164.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675944 sequences (100000022 bp) in 1.826 CPU sec, 1.828 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284807, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 346, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (363.11, 163.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284949, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (354.80, 162.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 165, 179)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (137, 207)
[M::mem_pestat] mean and std.dev: (171.00, 6.93)
[M::mem_pestat] low and high boundaries for proper pairs: (123, 221)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674218 reads in 210.721 CPU sec, 6.798 real sec
[M::mem_process_seqs] Processed 674796 reads in 161.637 CPU sec, 5.289 real sec
[M::mem_process_seqs] Processed 675906 reads in 176.492 CPU sec, 5.774 real sec
sorting block 1, #reads = 1079296 mem = 536880273 peak = 702050304
sorting block 2, #reads = 1080259 mem = 536881241 peak = 867401728
[M::process] read 676266 sequences (100000131 bp) in 2.275 CPU sec, 2.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285651, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 313, 2152)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6128)
[M::mem_pestat] mean and std.dev: (972.80, 961.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8116)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (354.45, 162.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675428 sequences (100000157 bp) in 1.373 CPU sec, 1.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 285240, 8, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 788, 788)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2092)
[M::mem_pestat] mean and std.dev: (502.85, 313.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2744)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (351.27, 161.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 668, 698)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1564)
[M::mem_pestat] mean and std.dev: (410.73, 226.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1997)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675944 reads in 149.535 CPU sec, 4.735 real sec
[M::process] read 673652 sequences (100000087 bp) in 1.613 CPU sec, 1.647 real sec...
[M::mem_process_seqs] Processed 676266 reads in 94.532 CPU sec, 2.972 real sec
sorting block 3, #reads = 1082107 mem = 536878533 peak = 867401728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284376, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (356.66, 163.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 436, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244)
[M::mem_pestat] mean and std.dev: (392.50, 250.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1598)
[M::mem_pestat] skip orientation RR
[M::process] read 675118 sequences (100000207 bp) in 2.035 CPU sec, 2.146 real sec...
[M::process] read 675990 sequences (100000073 bp) in 1.345 CPU sec, 1.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284573, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 358, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (374.16, 167.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675428 reads in 146.499 CPU sec, 4.660 real sec
[M::mem_process_seqs] Processed 673652 reads in 92.709 CPU sec, 2.954 real sec
sorting block 4, #reads = 1081277 mem = 536880399 peak = 867401728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285128, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 342, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (360.56, 163.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675324 sequences (100000120 bp) in 2.113 CPU sec, 2.246 real sec...
[M::process] read 675400 sequences (100000261 bp) in 1.540 CPU sec, 1.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284534, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (353.71, 163.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675118 reads in 150.456 CPU sec, 4.778 real sec
[M::mem_process_seqs] Processed 675990 reads in 95.783 CPU sec, 3.030 real sec
sorting block 5, #reads = 1081724 mem = 536876609 peak = 867401728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284197, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (357.43, 162.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675542 sequences (100000113 bp) in 2.503 CPU sec, 2.641 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284859, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (356.48, 162.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675104 sequences (100000139 bp) in 1.490 CPU sec, 1.498 real sec...
[M::mem_process_seqs] Processed 675324 reads in 151.607 CPU sec, 4.805 real sec
sorting block 6, #reads = 1080450 mem = 536878081 peak = 867401728
[M::mem_process_seqs] Processed 675400 reads in 96.857 CPU sec, 3.058 real sec
sorting block 7, #reads = 1080935 mem = 536879493 peak = 885129216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284825, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (354.70, 162.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674042 sequences (100000243 bp) in 2.193 CPU sec, 2.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284063, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 339, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (357.91, 162.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675536 sequences (100000293 bp) in 1.780 CPU sec, 1.779 real sec...
[M::mem_process_seqs] Processed 675542 reads in 153.608 CPU sec, 4.870 real sec
[M::mem_process_seqs] Processed 675104 reads in 99.608 CPU sec, 3.146 real sec
sorting block 8, #reads = 1081168 mem = 536878772 peak = 885129216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 283470, 1, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (352, 352, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (352, 352)
[M::mem_pestat] mean and std.dev: (352.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (352, 352)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 346, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (363.65, 162.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 258, 1440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3956)
[M::mem_pestat] mean and std.dev: (544.74, 538.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5214)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 675594 sequences (100000121 bp) in 2.365 CPU sec, 2.503 real sec...
[M::process] read 673770 sequences (100000061 bp) in 1.380 CPU sec, 1.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284113, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (349.96, 159.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674042 reads in 154.630 CPU sec, 4.914 real sec
[M::mem_process_seqs] Processed 675536 reads in 98.616 CPU sec, 3.127 real sec
sorting block 9, #reads = 1079863 mem = 536877417 peak = 885129216
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284258, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (350.80, 160.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675416 sequences (100000173 bp) in 2.622 CPU sec, 2.745 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284122, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 348, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (364.67, 162.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675594 reads in 153.306 CPU sec, 4.860 real sec
[M::mem_process_seqs] Processed 673770 reads in 96.982 CPU sec, 3.043 real sec
sorting block 10, #reads = 1081481 mem = 536881032 peak = 885129216
[M::process] read 675170 sequences (100000275 bp) in 2.293 CPU sec, 2.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283952, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (351.21, 159.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675412 sequences (100000226 bp) in 2.456 CPU sec, 2.601 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284760, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 331, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (350.69, 157.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674144 sequences (100000268 bp) in 1.640 CPU sec, 1.640 real sec...
[M::mem_process_seqs] Processed 675416 reads in 155.885 CPU sec, 4.945 real sec
sorting block 11, #reads = 1080558 mem = 536876961 peak = 885129216
[M::mem_process_seqs] Processed 675170 reads in 98.850 CPU sec, 3.119 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283691, 3, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (381, 510, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 768)
[M::mem_pestat] mean and std.dev: (444.88, 55.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 897)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (349.94, 159.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 12, #reads = 1081087 mem = 536877984 peak = 922566656
[M::process] read 674046 sequences (100000106 bp) in 2.407 CPU sec, 2.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283567, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 342, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (360.34, 160.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675412 reads in 156.551 CPU sec, 4.975 real sec
[M::process] read 675514 sequences (100000269 bp) in 1.889 CPU sec, 1.900 real sec...
[M::mem_process_seqs] Processed 674144 reads in 101.876 CPU sec, 3.209 real sec
sorting block 13, #reads = 1080157 mem = 536881522 peak = 922566656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283457, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 341, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (359.21, 159.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (283, 283, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (283, 283)
[M::mem_pestat] mean and std.dev: (283.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (283, 283)
[M::mem_pestat] skip orientation RR
[M::process] read 675382 sequences (100000037 bp) in 2.666 CPU sec, 2.816 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284381, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (348.68, 157.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674046 reads in 154.347 CPU sec, 4.894 real sec
[M::mem_process_seqs] Processed 675514 reads in 99.914 CPU sec, 3.141 real sec
[M::process] read 674670 sequences (100000050 bp) in 2.306 CPU sec, 2.367 real sec...
sorting block 14, #reads = 1080161 mem = 536877710 peak = 922566656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284302, 10, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (348.36, 158.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1497, 1497, 1497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1497, 1497)
[M::mem_pestat] mean and std.dev: (1497.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1497, 1497)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675012 sequences (100000081 bp) in 2.392 CPU sec, 2.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284064, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (355.20, 160.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675382 reads in 155.056 CPU sec, 4.936 real sec
[M::mem_process_seqs] Processed 674670 reads in 99.140 CPU sec, 3.118 real sec
[M::process] read 675266 sequences (100000074 bp) in 2.103 CPU sec, 2.171 real sec...
sorting block 15, #reads = 1081279 mem = 536882135 peak = 922566656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284522, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (353.86, 160.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675110 sequences (100000162 bp) in 2.559 CPU sec, 2.734 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284588, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (355.01, 162.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675012 reads in 152.530 CPU sec, 4.853 real sec
[M::process] read 674696 sequences (100000234 bp) in 1.505 CPU sec, 1.516 real sec...
sorting block 16, #reads = 1081719 mem = 536880939 peak = 922566656
[M::mem_process_seqs] Processed 675266 reads in 97.863 CPU sec, 3.088 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284256, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (357.34, 163.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 17, #reads = 1081749 mem = 536880446 peak = 970682368
[M::process] read 674826 sequences (100000000 bp) in 2.487 CPU sec, 2.650 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284037, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 342, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (362.43, 165.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675110 reads in 149.445 CPU sec, 4.751 real sec
[M::mem_process_seqs] Processed 674696 reads in 98.536 CPU sec, 3.105 real sec
[M::process] read 675720 sequences (100000153 bp) in 2.077 CPU sec, 2.158 real sec...
sorting block 18, #reads = 1080966 mem = 536876822 peak = 970682368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284767, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (360.47, 163.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675450 sequences (100000100 bp) in 2.476 CPU sec, 2.635 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284596, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (352.47, 160.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674826 reads in 145.433 CPU sec, 4.631 real sec
[M::process] read 674560 sequences (100000252 bp) in 1.573 CPU sec, 1.603 real sec...
[M::mem_process_seqs] Processed 675720 reads in 94.558 CPU sec, 2.984 real sec
sorting block 19, #reads = 1080766 mem = 536879860 peak = 970682368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284131, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (355.63, 161.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675640 sequences (100000170 bp) in 2.310 CPU sec, 2.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284585, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 347, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (364.07, 164.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675450 reads in 142.323 CPU sec, 4.545 real sec
[M::mem_process_seqs] Processed 674560 reads in 91.371 CPU sec, 2.882 real sec
[M::process] read 675342 sequences (100000171 bp) in 1.846 CPU sec, 1.907 real sec...
sorting block 20, #reads = 1081595 mem = 536877641 peak = 970682368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285137, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (356.52, 163.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (382, 907, 907)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1957)
[M::mem_pestat] mean and std.dev: (749.50, 240.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2482)
[M::mem_pestat] skip orientation RR
[M::process] read 675152 sequences (100000087 bp) in 2.330 CPU sec, 2.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284697, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 342, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (360.10, 164.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675640 reads in 143.181 CPU sec, 4.552 real sec
sorting block 21, #reads = 1081859 mem = 536878612 peak = 970682368
[M::mem_process_seqs] Processed 675342 reads in 93.264 CPU sec, 2.933 real sec
[M::process] read 675684 sequences (100000073 bp) in 2.138 CPU sec, 2.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284718, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 343, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (360.82, 164.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 22, #reads = 1083035 mem = 536878377 peak = 970682368
[M::process] read 675176 sequences (100000013 bp) in 2.222 CPU sec, 2.372 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284393, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (354.59, 161.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675152 reads in 143.021 CPU sec, 4.554 real sec
[M::mem_process_seqs] Processed 675684 reads in 94.644 CPU sec, 2.979 real sec
[M::process] read 674198 sequences (100000231 bp) in 2.120 CPU sec, 2.207 real sec...
sorting block 23, #reads = 1082606 mem = 536880867 peak = 970682368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284843, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 342, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (360.49, 164.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675470 sequences (100000045 bp) in 2.330 CPU sec, 2.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284127, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 348, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (365.27, 163.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675176 reads in 143.391 CPU sec, 4.561 real sec
[M::mem_process_seqs] Processed 674198 reads in 94.288 CPU sec, 2.969 real sec
[M::process] read 675650 sequences (100000174 bp) in 1.701 CPU sec, 1.738 real sec...
sorting block 24, #reads = 1082939 mem = 536878313 peak = 970682368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285207, 2, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 276, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756)
[M::mem_pestat] mean and std.dev: (237.50, 93.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 337, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (355.14, 161.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675498 sequences (100000055 bp) in 2.591 CPU sec, 2.734 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284664, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 337, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (356.56, 163.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675470 reads in 145.321 CPU sec, 4.630 real sec
[M::mem_process_seqs] Processed 675650 reads in 93.127 CPU sec, 2.936 real sec
sorting block 25, #reads = 1082061 mem = 536879369 peak = 970682368
[M::process] read 675430 sequences (100000129 bp) in 2.177 CPU sec, 2.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284897, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (356.50, 162.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675118 sequences (100000156 bp) in 2.508 CPU sec, 2.648 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284992, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 337, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (356.51, 162.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675498 reads in 143.446 CPU sec, 4.561 real sec
sorting block 26, #reads = 1083163 mem = 536877722 peak = 970682368
[M::mem_process_seqs] Processed 675430 reads in 92.434 CPU sec, 2.906 real sec
[M::process] read 674326 sequences (100000236 bp) in 2.310 CPU sec, 2.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284834, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (357.55, 163.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 27, #reads = 1083003 mem = 536879464 peak = 970682368
[M::process] read 673410 sequences (100000268 bp) in 2.368 CPU sec, 2.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283556, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 343, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (361.53, 162.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675118 reads in 146.409 CPU sec, 4.670 real sec
[M::mem_process_seqs] Processed 674326 reads in 95.147 CPU sec, 3.000 real sec
[M::process] read 675132 sequences (100000075 bp) in 2.417 CPU sec, 2.562 real sec...
sorting block 28, #reads = 1082016 mem = 536880905 peak = 970682368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284108, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 354, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (370.75, 165.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675350 sequences (100000117 bp) in 2.503 CPU sec, 2.664 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284466, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (354.55, 161.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673410 reads in 146.730 CPU sec, 4.675 real sec
[M::mem_process_seqs] Processed 675132 reads in 96.975 CPU sec, 3.054 real sec
sorting block 29, #reads = 1080848 mem = 536879078 peak = 970682368
[M::process] read 675050 sequences (100000061 bp) in 2.576 CPU sec, 2.747 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284855, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (352.77, 161.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675290 sequences (100000291 bp) in 2.526 CPU sec, 2.640 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284700, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 338, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (358.91, 165.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 315, 865)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2049)
[M::mem_pestat] mean and std.dev: (444.22, 320.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2641)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675350 reads in 145.934 CPU sec, 4.652 real sec
[M::mem_process_seqs] Processed 675050 reads in 95.048 CPU sec, 2.995 real sec
sorting block 30, #reads = 1081657 mem = 536877689 peak = 970682368
[M::process] read 675404 sequences (100000190 bp) in 2.754 CPU sec, 2.925 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284805, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (355.54, 163.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 284689, 3, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (245, 340, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (312.50, 123.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1081)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (354.05, 162.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675246 sequences (100000056 bp) in 2.345 CPU sec, 2.444 real sec...
[M::mem_process_seqs] Processed 675290 reads in 147.907 CPU sec, 4.719 real sec
sorting block 31, #reads = 1082362 mem = 536878380 peak = 970682368
[M::mem_process_seqs] Processed 675404 reads in 95.360 CPU sec, 3.013 real sec
[M::process] read 673766 sequences (100000259 bp) in 2.492 CPU sec, 2.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284666, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 329, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (349.20, 158.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 32, #reads = 1081479 mem = 536879719 peak = 970682368
[M::process] read 674778 sequences (100000172 bp) in 1.991 CPU sec, 2.091 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284623, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (364.98, 163.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675246 reads in 148.650 CPU sec, 4.739 real sec
[M::mem_process_seqs] Processed 673766 reads in 97.035 CPU sec, 3.061 real sec
[M::process] read 675494 sequences (100000067 bp) in 2.166 CPU sec, 2.314 real sec...
sorting block 33, #reads = 1080873 mem = 536878441 peak = 970682368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284967, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (355.89, 161.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675306 sequences (100000259 bp) in 2.426 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284880, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (350.65, 160.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674778 reads in 151.140 CPU sec, 4.804 real sec
[M::mem_process_seqs] Processed 675494 reads in 97.546 CPU sec, 3.069 real sec
[M::process] read 675366 sequences (100000065 bp) in 1.774 CPU sec, 1.835 real sec...
sorting block 34, #reads = 1080513 mem = 536879408 peak = 970682368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285046, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (352.43, 161.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675364 sequences (100000025 bp) in 2.524 CPU sec, 2.681 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284293, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (350.52, 160.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675306 reads in 148.102 CPU sec, 4.712 real sec
[M::mem_process_seqs] Processed 675366 reads in 95.268 CPU sec, 2.994 real sec
sorting block 35, #reads = 1083192 mem = 536882622 peak = 970682368
[M::process] read 675618 sequences (100000236 bp) in 2.148 CPU sec, 2.240 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285073, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (261, 284, 1290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3348)
[M::mem_pestat] mean and std.dev: (649.33, 828.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4377)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (352.01, 160.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 492, 646)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1486)
[M::mem_pestat] mean and std.dev: (472.90, 291.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1906)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 36, #reads = 1081050 mem = 536879119 peak = 987783168
[M::process] read 675444 sequences (100000165 bp) in 2.343 CPU sec, 2.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285191, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (355.65, 164.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675364 reads in 153.538 CPU sec, 4.879 real sec
[M::mem_process_seqs] Processed 675618 reads in 98.903 CPU sec, 3.112 real sec
[M::process] read 675292 sequences (100000056 bp) in 2.036 CPU sec, 2.064 real sec...
sorting block 37, #reads = 1080889 mem = 536878174 peak = 987783168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285066, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (290, 487, 1619)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4277)
[M::mem_pestat] mean and std.dev: (1031.36, 927.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5606)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (354.80, 163.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676522 sequences (100000235 bp) in 2.555 CPU sec, 2.695 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284942, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (357.61, 165.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675444 reads in 151.547 CPU sec, 4.812 real sec
[M::mem_process_seqs] Processed 675292 reads in 99.056 CPU sec, 3.111 real sec
[M::process] read 675738 sequences (100000257 bp) in 2.235 CPU sec, 2.322 real sec...
sorting block 38, #reads = 1081079 mem = 536880320 peak = 987783168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285412, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 327, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (344.09, 154.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676032 sequences (100000132 bp) in 2.564 CPU sec, 2.703 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284660, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (347.35, 154.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676522 reads in 153.115 CPU sec, 4.879 real sec
[M::mem_process_seqs] Processed 675738 reads in 97.835 CPU sec, 3.078 real sec
[M::process] read 675788 sequences (100000081 bp) in 2.238 CPU sec, 2.331 real sec...
sorting block 39, #reads = 1080247 mem = 536880368 peak = 987783168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284390, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (347.42, 156.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675480 sequences (100000086 bp) in 2.421 CPU sec, 2.554 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284961, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 332, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (348.61, 155.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676032 reads in 152.657 CPU sec, 4.872 real sec
[M::mem_process_seqs] Processed 675788 reads in 99.579 CPU sec, 3.144 real sec
sorting block 40, #reads = 1078943 mem = 536878583 peak = 987783168
[M::process] read 675142 sequences (100000247 bp) in 2.206 CPU sec, 2.293 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285032, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (350.64, 155.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1079842 mem = 536879588 peak = 1006350336
[M::process] read 673516 sequences (100000166 bp) in 2.673 CPU sec, 2.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284714, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 341, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (357.50, 159.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675480 reads in 149.355 CPU sec, 4.749 real sec
[M::mem_process_seqs] Processed 675142 reads in 98.104 CPU sec, 3.089 real sec
[M::process] read 674548 sequences (100000156 bp) in 2.237 CPU sec, 2.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284334, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 359, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (372.03, 162.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 42, #reads = 1081165 mem = 536876883 peak = 1006350336
[M::process] read 676038 sequences (100000074 bp) in 2.401 CPU sec, 2.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284462, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 345, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (360.38, 159.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673516 reads in 147.788 CPU sec, 4.706 real sec
[M::mem_process_seqs] Processed 674548 reads in 97.471 CPU sec, 3.066 real sec
[M::process] read 675864 sequences (100000115 bp) in 2.278 CPU sec, 2.359 real sec...
sorting block 43, #reads = 1079866 mem = 536879992 peak = 1006350336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285043, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (349.40, 157.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675610 sequences (100000135 bp) in 2.626 CPU sec, 2.769 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284597, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 336, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (354.11, 160.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676038 reads in 150.539 CPU sec, 4.790 real sec
[M::mem_process_seqs] Processed 675864 reads in 96.481 CPU sec, 3.041 real sec
[M::process] read 675614 sequences (100000219 bp) in 2.258 CPU sec, 2.375 real sec...
sorting block 44, #reads = 1082127 mem = 536878347 peak = 1006350336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284780, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (353.16, 159.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675596 sequences (100000030 bp) in 2.401 CPU sec, 2.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284595, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (352.23, 158.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675610 reads in 150.921 CPU sec, 4.863 real sec
[M::mem_process_seqs] Processed 675614 reads in 98.162 CPU sec, 3.088 real sec
sorting block 45, #reads = 1082542 mem = 536878721 peak = 1006350336
[M::process] read 675452 sequences (100000222 bp) in 2.427 CPU sec, 2.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284770, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (353.13, 159.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1081309 mem = 536879725 peak = 1006350336
[M::process] read 675534 sequences (100000209 bp) in 2.524 CPU sec, 2.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284501, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (356.24, 162.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 207, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 379)
[M::mem_pestat] mean and std.dev: (181.50, 42.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 448)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675596 reads in 152.271 CPU sec, 4.843 real sec
[M::mem_process_seqs] Processed 675452 reads in 98.462 CPU sec, 3.101 real sec
[M::process] read 673658 sequences (100000223 bp) in 2.214 CPU sec, 2.320 real sec...
sorting block 47, #reads = 1081594 mem = 536878930 peak = 1006350336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284857, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 338, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (356.06, 161.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674306 sequences (100000017 bp) in 2.134 CPU sec, 2.279 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283601, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (365.50, 160.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675534 reads in 149.580 CPU sec, 4.762 real sec
[M::mem_process_seqs] Processed 673658 reads in 95.372 CPU sec, 3.002 real sec
[M::process] read 675328 sequences (100000016 bp) in 2.121 CPU sec, 2.190 real sec...
sorting block 48, #reads = 1080661 mem = 536879666 peak = 1006350336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283965, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (362.40, 161.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675104 sequences (100000279 bp) in 2.623 CPU sec, 2.753 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284630, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (352.89, 159.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 758, 854)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2128)
[M::mem_pestat] mean and std.dev: (562.00, 299.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2765)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674306 reads in 150.947 CPU sec, 4.796 real sec
[M::mem_process_seqs] Processed 675328 reads in 98.505 CPU sec, 3.100 real sec
[M::process] read 675110 sequences (100000013 bp) in 2.203 CPU sec, 2.284 real sec...
sorting block 49, #reads = 1079934 mem = 536882081 peak = 1006350336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284721, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 336, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (355.91, 161.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675188 sequences (100000144 bp) in 2.254 CPU sec, 2.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284449, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (354.80, 161.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675104 reads in 154.340 CPU sec, 4.908 real sec
[M::mem_process_seqs] Processed 675110 reads in 99.109 CPU sec, 3.119 real sec
[M::process] read 675232 sequences (100000174 bp) in 2.012 CPU sec, 2.056 real sec...
sorting block 50, #reads = 1080710 mem = 536879395 peak = 1006350336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283927, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (353.58, 161.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 51, #reads = 1081525 mem = 536876739 peak = 1084018688
[M::process] read 675606 sequences (100000123 bp) in 2.682 CPU sec, 2.803 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284475, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (351.60, 159.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675188 reads in 155.113 CPU sec, 4.937 real sec
[M::mem_process_seqs] Processed 675232 reads in 99.759 CPU sec, 3.138 real sec
[M::process] read 675486 sequences (100000102 bp) in 2.045 CPU sec, 2.102 real sec...
sorting block 52, #reads = 1081098 mem = 536878486 peak = 1084018688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 283959, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (289, 324, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (19, 694)
[M::mem_pestat] mean and std.dev: (299.27, 124.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 327, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (347.87, 158.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675636 sequences (100000188 bp) in 2.323 CPU sec, 2.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283985, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (351.39, 160.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675606 reads in 153.406 CPU sec, 4.877 real sec
[M::process] read 675394 sequences (100000129 bp) in 1.834 CPU sec, 1.855 real sec...
[M::mem_process_seqs] Processed 675486 reads in 99.172 CPU sec, 3.122 real sec
sorting block 53, #reads = 1081797 mem = 536878407 peak = 1084018688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284255, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (349.00, 159.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675470 sequences (100000147 bp) in 2.509 CPU sec, 2.650 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284125, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (350.24, 160.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675256 sequences (100000033 bp) in 1.690 CPU sec, 1.690 real sec...
[M::mem_process_seqs] Processed 675636 reads in 154.292 CPU sec, 4.895 real sec
[M::mem_process_seqs] Processed 675394 reads in 100.894 CPU sec, 3.188 real sec
sorting block 54, #reads = 1081910 mem = 536877462 peak = 1084018688
[M::process] read 675534 sequences (100000241 bp) in 2.393 CPU sec, 2.540 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284538, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (352.94, 161.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675256 reads in 86.786 CPU sec, 2.728 real sec
[M::process] read 675414 sequences (100000039 bp) in 1.831 CPU sec, 1.853 real sec...
sorting block 55, #reads = 1081561 mem = 536880186 peak = 1084018688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284867, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (350.94, 160.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285222, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (351.83, 161.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675470 reads in 240.328 CPU sec, 7.659 real sec
[M::mem_process_seqs] Processed 675534 reads in 116.615 CPU sec, 3.706 real sec
[M::process] read 675674 sequences (100000169 bp) in 1.983 CPU sec, 2.031 real sec...
sorting block 56, #reads = 1081832 mem = 536878599 peak = 1084018688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284551, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (353.97, 163.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675698 sequences (100000110 bp) in 2.835 CPU sec, 2.994 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285032, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (353.91, 165.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675414 reads in 155.681 CPU sec, 4.968 real sec
[M::mem_process_seqs] Processed 675674 reads in 103.281 CPU sec, 3.256 real sec
[M::process] read 674892 sequences (100000161 bp) in 2.028 CPU sec, 2.118 real sec...
sorting block 57, #reads = 1081347 mem = 536877040 peak = 1084018688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284302, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (350.01, 158.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674488 sequences (100000026 bp) in 2.644 CPU sec, 2.828 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 283468, 3, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 180, 3341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9673)
[M::mem_pestat] mean and std.dev: (1483.33, 1570.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12839)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 336, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (351.70, 155.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675698 reads in 154.938 CPU sec, 4.947 real sec
sorting block 58, #reads = 1081228 mem = 536878035 peak = 1088606208
[M::mem_process_seqs] Processed 674892 reads in 100.124 CPU sec, 3.155 real sec
[M::process] read 674656 sequences (100000146 bp) in 1.975 CPU sec, 2.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283582, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 341, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (357.10, 157.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 59, #reads = 1080497 mem = 536881516 peak = 1117757440
[M::process] read 675252 sequences (100000088 bp) in 2.343 CPU sec, 2.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284302, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 340, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (355.10, 156.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674488 reads in 151.979 CPU sec, 4.859 real sec
[M::mem_process_seqs] Processed 674656 reads in 99.132 CPU sec, 3.124 real sec
[M::process] read 675082 sequences (100000245 bp) in 2.152 CPU sec, 2.264 real sec...
sorting block 60, #reads = 1080253 mem = 536883560 peak = 1117757440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284233, 10, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 312, 1077)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3001)
[M::mem_pestat] mean and std.dev: (637.90, 806.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3963)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 335, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (351.55, 155.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (3, 151, 1682)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5040)
[M::mem_pestat] mean and std.dev: (636.90, 760.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6719)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (427, 778, 778)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1480)
[M::mem_pestat] mean and std.dev: (558.62, 169.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1831)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 675236 sequences (100000226 bp) in 2.422 CPU sec, 2.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284127, 4, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (807, 1223, 8974)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25308)
[M::mem_pestat] mean and std.dev: (3377.60, 3687.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 33475)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 332, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (349.56, 154.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675252 reads in 149.991 CPU sec, 4.801 real sec
[M::mem_process_seqs] Processed 675082 reads in 96.548 CPU sec, 3.045 real sec
[M::process] read 673462 sequences (100000052 bp) in 2.171 CPU sec, 2.315 real sec...
sorting block 61, #reads = 1081679 mem = 536880454 peak = 1117757440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283837, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 301, 769)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1897)
[M::mem_pestat] mean and std.dev: (363.85, 230.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2461)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (351.69, 156.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674056 sequences (100000033 bp) in 2.363 CPU sec, 2.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283202, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 354, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (368.35, 161.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675236 reads in 152.220 CPU sec, 4.868 real sec
[M::mem_process_seqs] Processed 673462 reads in 98.417 CPU sec, 3.103 real sec
sorting block 62, #reads = 1081462 mem = 536878105 peak = 1117757440
[M::process] read 675786 sequences (100000263 bp) in 1.968 CPU sec, 2.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284328, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (363.49, 159.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 63, #reads = 1080145 mem = 536879902 peak = 1117757440
[M::process] read 675566 sequences (100000020 bp) in 2.716 CPU sec, 2.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284312, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (352.10, 159.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674056 reads in 157.379 CPU sec, 5.040 real sec
[M::mem_process_seqs] Processed 675786 reads in 103.598 CPU sec, 3.273 real sec
[M::process] read 675832 sequences (100000270 bp) in 2.279 CPU sec, 2.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284595, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 196, 225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (117, 297)
[M::mem_pestat] mean and std.dev: (198.14, 11.38)
[M::mem_pestat] low and high boundaries for proper pairs: (81, 333)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 336, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (353.73, 159.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 236, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746)
[M::mem_pestat] mean and std.dev: (257.00, 94.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 64, #reads = 1081442 mem = 536881615 peak = 1117757440
[M::process] read 675802 sequences (100000159 bp) in 2.136 CPU sec, 2.293 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284583, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (349.59, 157.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675566 reads in 160.154 CPU sec, 5.111 real sec
[M::process] read 675864 sequences (100000003 bp) in 1.552 CPU sec, 1.564 real sec...
[M::mem_process_seqs] Processed 675832 reads in 105.290 CPU sec, 3.327 real sec
sorting block 65, #reads = 1081354 mem = 536879855 peak = 1117757440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284396, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (348.55, 157.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675842 sequences (100000007 bp) in 2.847 CPU sec, 3.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284899, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (351.17, 159.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 250, 790)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2106)
[M::mem_pestat] mean and std.dev: (379.09, 277.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2764)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675802 reads in 158.358 CPU sec, 5.044 real sec
[M::mem_process_seqs] Processed 675864 reads in 103.440 CPU sec, 3.259 real sec
[M::process] read 675668 sequences (100000048 bp) in 1.938 CPU sec, 1.989 real sec...
sorting block 66, #reads = 1082150 mem = 536878330 peak = 1117757440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284226, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (351.00, 158.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674180 sequences (100000010 bp) in 2.313 CPU sec, 2.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284148, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (352.51, 159.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675842 reads in 156.458 CPU sec, 4.985 real sec
[M::process] read 674674 sequences (100000164 bp) in 1.906 CPU sec, 1.921 real sec...
sorting block 67, #reads = 1082460 mem = 536889293 peak = 1117757440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 284033, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 134, 183)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327)
[M::mem_pestat] mean and std.dev: (150.27, 48.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 399)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (364.41, 161.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675588 sequences (100000074 bp) in 1.891 CPU sec, 1.893 real sec...
[M::mem_process_seqs] Processed 674180 reads in 89.652 CPU sec, 3.004 real sec
sorting block 68, #reads = 1080664 mem = 536880955 peak = 1117757440
[M::mem_process_seqs] Processed 675668 reads in 232.223 CPU sec, 7.522 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284119, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 343, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (359.76, 160.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675432 sequences (100000273 bp) in 2.647 CPU sec, 2.978 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284014, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (353.14, 160.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (889, 889, 1022)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (623, 1288)
[M::mem_pestat] mean and std.dev: (946.09, 94.95)
[M::mem_pestat] low and high boundaries for proper pairs: (490, 1421)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674674 reads in 176.115 CPU sec, 5.613 real sec
[M::mem_process_seqs] Processed 675588 reads in 105.558 CPU sec, 3.347 real sec
sorting block 69, #reads = 1081796 mem = 536878121 peak = 1117757440
[M::process] read 674628 sequences (100000243 bp) in 2.398 CPU sec, 2.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284293, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (353.20, 159.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674882 sequences (100000039 bp) in 2.180 CPU sec, 2.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283387, 7, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (276, 431, 597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1239)
[M::mem_pestat] mean and std.dev: (388.83, 122.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1560)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 342, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (360.18, 161.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675432 reads in 162.624 CPU sec, 5.174 real sec
sorting block 70, #reads = 1081931 mem = 536878252 peak = 1117757440
[M::mem_process_seqs] Processed 674628 reads in 107.592 CPU sec, 3.388 real sec
[M::process] read 675296 sequences (100000019 bp) in 2.086 CPU sec, 2.138 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284233, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (358.89, 161.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2304, 2304, 2501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1910, 2895)
[M::mem_pestat] mean and std.dev: (2325.89, 61.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1713, 3092)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 71, #reads = 1081010 mem = 536879195 peak = 1117757440
[M::process] read 675366 sequences (100000301 bp) in 2.413 CPU sec, 2.600 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284819, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (353.78, 159.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674882 reads in 161.603 CPU sec, 5.163 real sec
[M::process] read 675766 sequences (100000123 bp) in 1.943 CPU sec, 1.990 real sec...
[M::mem_process_seqs] Processed 675296 reads in 106.948 CPU sec, 3.369 real sec
sorting block 72, #reads = 1081147 mem = 536880282 peak = 1117757440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284576, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (352.64, 159.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675700 sequences (100000222 bp) in 2.689 CPU sec, 2.863 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284752, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (348.41, 157.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675366 reads in 161.491 CPU sec, 5.158 real sec
[M::process] read 675506 sequences (100000146 bp) in 1.965 CPU sec, 2.004 real sec...
[M::mem_process_seqs] Processed 675766 reads in 106.663 CPU sec, 3.364 real sec
sorting block 73, #reads = 1081093 mem = 536881721 peak = 1117757440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284594, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (348.99, 158.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675872 sequences (100000236 bp) in 2.491 CPU sec, 2.679 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284389, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (348.72, 158.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675700 reads in 162.677 CPU sec, 5.195 real sec
[M::process] read 675606 sequences (100000012 bp) in 1.843 CPU sec, 1.879 real sec...
[M::mem_process_seqs] Processed 675506 reads in 105.549 CPU sec, 3.335 real sec
sorting block 74, #reads = 1081330 mem = 536881012 peak = 1117757440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284935, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (348.36, 158.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675446 sequences (100000055 bp) in 2.610 CPU sec, 2.777 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284170, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (352.19, 161.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675872 reads in 159.464 CPU sec, 5.090 real sec
sorting block 75, #reads = 1080089 mem = 536879543 peak = 1117757440
[M::process] read 675398 sequences (100000264 bp) in 1.906 CPU sec, 1.933 real sec...
[M::mem_process_seqs] Processed 675606 reads in 102.877 CPU sec, 3.243 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284564, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (352.55, 161.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 76, #reads = 1080717 mem = 536878196 peak = 1117757440
[M::process] read 675916 sequences (100000186 bp) in 2.542 CPU sec, 2.732 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284194, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (352.10, 160.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675446 reads in 159.145 CPU sec, 5.072 real sec
[M::mem_process_seqs] Processed 675398 reads in 102.900 CPU sec, 3.236 real sec
[M::process] read 678524 sequences (100000157 bp) in 2.121 CPU sec, 2.187 real sec...
sorting block 77, #reads = 1080970 mem = 536877555 peak = 1117757440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285169, 11, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (349.33, 161.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2304, 2304, 2304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2304, 2304)
[M::mem_pestat] mean and std.dev: (2304.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (2304, 2304)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 250, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 504)
[M::mem_pestat] mean and std.dev: (214.00, 71.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 604)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 676054 sequences (100000237 bp) in 2.689 CPU sec, 2.883 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285651, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 313, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (340.40, 163.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675916 reads in 165.596 CPU sec, 5.277 real sec
[M::process] read 676078 sequences (100000093 bp) in 1.921 CPU sec, 1.953 real sec...
[M::mem_process_seqs] Processed 678524 reads in 111.576 CPU sec, 3.518 real sec
sorting block 78, #reads = 1080978 mem = 536880254 peak = 1117757440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286251, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 343, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (362.54, 169.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677602 sequences (100000244 bp) in 2.637 CPU sec, 2.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285589, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 340, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (360.20, 167.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676054 reads in 167.526 CPU sec, 5.341 real sec
[M::process] read 677530 sequences (100000009 bp) in 2.000 CPU sec, 2.029 real sec...
[M::mem_process_seqs] Processed 676078 reads in 108.578 CPU sec, 3.418 real sec
sorting block 79, #reads = 1077626 mem = 536879105 peak = 1117757440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285644, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 321, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (344.72, 161.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677742 sequences (100000154 bp) in 2.843 CPU sec, 3.056 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285530, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (346.85, 163.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677602 reads in 167.587 CPU sec, 5.340 real sec
sorting block 80, #reads = 1077705 mem = 536878850 peak = 1117757440
[M::mem_process_seqs] Processed 677530 reads in 109.335 CPU sec, 3.442 real sec
[M::process] read 677056 sequences (100000242 bp) in 2.093 CPU sec, 2.142 real sec...
sorting block 81, #reads = 1079348 mem = 536884176 peak = 1129357312
[M::process] read 674924 sequences (100000237 bp) in 2.588 CPU sec, 2.789 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285629, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 327, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (349.22, 162.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677056 reads in 95.403 CPU sec, 2.997 real sec
[M::process] read 677438 sequences (100000232 bp) in 2.067 CPU sec, 2.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285826, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 319, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (342.90, 161.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 284523, 5, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (325, 430, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (115, 640)
[M::mem_pestat] mean and std.dev: (372.13, 68.63)
[M::mem_pestat] low and high boundaries for proper pairs: (10, 745)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 347, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (364.39, 165.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 677742 reads in 256.809 CPU sec, 8.175 real sec
[M::mem_process_seqs] Processed 674924 reads in 123.319 CPU sec, 3.901 real sec
sorting block 82, #reads = 1079269 mem = 536880696 peak = 1129357312
[M::process] read 677790 sequences (100000036 bp) in 2.487 CPU sec, 2.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285177, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 323, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (345.68, 161.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 83, #reads = 1078319 mem = 536879523 peak = 1129357312
[M::process] read 677314 sequences (100000035 bp) in 2.571 CPU sec, 2.769 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286345, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 320, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (342.57, 160.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677438 reads in 168.855 CPU sec, 5.387 real sec
[M::mem_process_seqs] Processed 677790 reads in 109.246 CPU sec, 3.443 real sec
[M::process] read 676734 sequences (100000103 bp) in 2.171 CPU sec, 2.235 real sec...
sorting block 84, #reads = 1079303 mem = 536880028 peak = 1129357312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285204, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (345.61, 160.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675938 sequences (100000233 bp) in 2.527 CPU sec, 2.719 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 285689, 3, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 196, 7937)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23419)
[M::mem_pestat] mean and std.dev: (3010.91, 3725.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 31160)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 330, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (351.11, 162.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 677314 reads in 170.436 CPU sec, 5.436 real sec
[M::process] read 674396 sequences (100000078 bp) in 2.025 CPU sec, 2.048 real sec...
[M::mem_process_seqs] Processed 676734 reads in 111.215 CPU sec, 3.503 real sec
sorting block 85, #reads = 1078382 mem = 536881101 peak = 1129357312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284610, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (356.76, 163.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676166 sequences (100000107 bp) in 2.681 CPU sec, 2.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284923, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 405, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (311.78, 179.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 352, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (368.28, 166.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675914 sequences (100000005 bp) in 1.766 CPU sec, 1.777 real sec...
[M::mem_process_seqs] Processed 675938 reads in 169.644 CPU sec, 5.402 real sec
[M::mem_process_seqs] Processed 674396 reads in 110.667 CPU sec, 3.499 real sec
sorting block 86, #reads = 1078214 mem = 536879043 peak = 1129357312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284882, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 336, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (355.73, 164.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674850 sequences (100000291 bp) in 2.581 CPU sec, 2.747 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285541, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (353.76, 161.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675662 sequences (100000013 bp) in 1.847 CPU sec, 1.852 real sec...
[M::mem_process_seqs] Processed 676166 reads in 172.541 CPU sec, 5.488 real sec
sorting block 87, #reads = 1077269 mem = 536878515 peak = 1129357312
[M::mem_process_seqs] Processed 675914 reads in 110.886 CPU sec, 3.501 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284635, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 334, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (304.31, 94.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 343, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (360.76, 162.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 88, #reads = 1077931 mem = 536885760 peak = 1136660480
[M::process] read 675580 sequences (100000123 bp) in 2.810 CPU sec, 3.010 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285353, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 332, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (350.49, 158.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675174 sequences (100000145 bp) in 1.872 CPU sec, 1.880 real sec...
[M::mem_process_seqs] Processed 674850 reads in 172.141 CPU sec, 5.474 real sec
[M::mem_process_seqs] Processed 675662 reads in 112.403 CPU sec, 3.551 real sec
sorting block 89, #reads = 1076946 mem = 536877968 peak = 1136660480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284295, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (352.41, 159.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674200 sequences (100000219 bp) in 2.479 CPU sec, 2.665 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284984, 1, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 340, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (358.67, 162.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 418, 2465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7019)
[M::mem_pestat] mean and std.dev: (889.93, 1024.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9296)
[M::mem_pestat] skip orientation RR
[M::process] read 675868 sequences (100000215 bp) in 1.910 CPU sec, 1.919 real sec...
[M::mem_process_seqs] Processed 675580 reads in 170.374 CPU sec, 5.417 real sec
[M::mem_process_seqs] Processed 675174 reads in 110.160 CPU sec, 3.477 real sec
sorting block 90, #reads = 1077296 mem = 536877691 peak = 1136660480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284528, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 349, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (366.11, 163.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675876 sequences (100000202 bp) in 2.685 CPU sec, 2.867 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284932, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (350.53, 159.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674972 sequences (100000113 bp) in 1.799 CPU sec, 1.800 real sec...
[M::mem_process_seqs] Processed 674200 reads in 173.155 CPU sec, 5.508 real sec
[M::mem_process_seqs] Processed 675868 reads in 113.173 CPU sec, 3.580 real sec
sorting block 91, #reads = 1077040 mem = 536879281 peak = 1136660480
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285031, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (349.64, 158.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675520 sequences (100000254 bp) in 2.419 CPU sec, 2.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284744, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (358.79, 162.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675642 sequences (100000270 bp) in 1.686 CPU sec, 1.696 real sec...
[M::mem_process_seqs] Processed 675876 reads in 169.176 CPU sec, 5.386 real sec
sorting block 92, #reads = 1077179 mem = 536876495 peak = 1136660480
[M::mem_process_seqs] Processed 674972 reads in 110.194 CPU sec, 3.483 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284707, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (355.52, 163.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (1607, 2000, 2050)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (721, 2936)
[M::mem_pestat] mean and std.dev: (1929.33, 173.67)
[M::mem_pestat] low and high boundaries for proper pairs: (278, 3379)
[M::mem_pestat] skip orientation RR
sorting block 93, #reads = 1077072 mem = 536878068 peak = 1169883136
[M::process] read 675762 sequences (100000111 bp) in 2.886 CPU sec, 3.046 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285189, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 332, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (353.92, 162.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675520 reads in 166.426 CPU sec, 5.291 real sec
[M::process] read 674930 sequences (100000009 bp) in 2.041 CPU sec, 2.067 real sec...
[M::mem_process_seqs] Processed 675642 reads in 109.813 CPU sec, 3.459 real sec
sorting block 94, #reads = 1077790 mem = 536879328 peak = 1169883136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285202, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (355.96, 164.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679112 sequences (100000254 bp) in 2.390 CPU sec, 2.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284234, 12, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 345, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (364.93, 168.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1070, 2397, 2397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5051)
[M::mem_pestat] mean and std.dev: (1495.75, 970.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6378)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 678980 sequences (100000026 bp) in 1.683 CPU sec, 1.690 real sec...
[M::mem_process_seqs] Processed 675762 reads in 167.522 CPU sec, 5.343 real sec
[M::mem_process_seqs] Processed 674930 reads in 111.027 CPU sec, 3.517 real sec
sorting block 95, #reads = 1078447 mem = 536877416 peak = 1169883136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287266, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 308, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (331.61, 154.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678128 sequences (100000024 bp) in 2.864 CPU sec, 3.046 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286834, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 311, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (332.51, 153.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678224 sequences (100000059 bp) in 1.910 CPU sec, 1.912 real sec...
[M::mem_process_seqs] Processed 679112 reads in 185.551 CPU sec, 5.905 real sec
sorting block 96, #reads = 1076083 mem = 536880307 peak = 1169883136
[M::mem_process_seqs] Processed 678980 reads in 122.931 CPU sec, 3.887 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286729, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 318, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (336.60, 153.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678028 sequences (100000138 bp) in 2.513 CPU sec, 2.619 real sec...
[M::process] read 678304 sequences (100000111 bp) in 1.923 CPU sec, 1.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286126, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 316, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (335.63, 153.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678128 reads in 182.500 CPU sec, 5.799 real sec
sorting block 97, #reads = 1071426 mem = 536881017 peak = 1169883136
[M::mem_process_seqs] Processed 678224 reads in 119.326 CPU sec, 3.782 real sec
sorting block 98, #reads = 1073080 mem = 536880105 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287013, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 318, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (337.56, 154.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (577, 577, 2225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5521)
[M::mem_pestat] mean and std.dev: (1156.08, 853.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7169)
[M::mem_pestat] skip orientation RR
[M::process] read 676650 sequences (100000299 bp) in 2.872 CPU sec, 3.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286349, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (333.58, 152.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677380 sequences (100000274 bp) in 1.683 CPU sec, 1.692 real sec...
[M::mem_process_seqs] Processed 678028 reads in 177.954 CPU sec, 5.657 real sec
[M::mem_process_seqs] Processed 678304 reads in 117.982 CPU sec, 3.737 real sec
sorting block 99, #reads = 1074882 mem = 536878325 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286301, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 328, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (346.42, 157.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677892 sequences (100000009 bp) in 2.555 CPU sec, 2.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285883, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 321, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (341.23, 156.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676650 reads in 166.677 CPU sec, 5.298 real sec
[M::process] read 678112 sequences (100000232 bp) in 1.910 CPU sec, 1.913 real sec...
[M::mem_process_seqs] Processed 677380 reads in 108.841 CPU sec, 3.433 real sec
sorting block 100, #reads = 1075565 mem = 536881260 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286076, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 316, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (337.24, 155.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677732 sequences (100000114 bp) in 2.838 CPU sec, 2.970 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286072, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 317, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (337.52, 155.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676936 sequences (100000189 bp) in 1.848 CPU sec, 1.851 real sec...
[M::mem_process_seqs] Processed 677892 reads in 168.716 CPU sec, 5.357 real sec
sorting block 101, #reads = 1077449 mem = 536878771 peak = 1174269952
[M::mem_process_seqs] Processed 678112 reads in 109.912 CPU sec, 3.473 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285896, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 321, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (341.87, 158.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 102, #reads = 1078586 mem = 536878205 peak = 1174269952
[M::process] read 674844 sequences (100000125 bp) in 2.527 CPU sec, 2.671 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285976, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (347.61, 160.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676500 sequences (100000238 bp) in 1.905 CPU sec, 1.907 real sec...
[M::mem_process_seqs] Processed 677732 reads in 172.077 CPU sec, 5.478 real sec
[M::mem_process_seqs] Processed 676936 reads in 113.361 CPU sec, 3.580 real sec
sorting block 103, #reads = 1076927 mem = 536882565 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285203, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 350, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (365.79, 165.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676314 sequences (100000042 bp) in 2.781 CPU sec, 2.924 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285880, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (352.95, 163.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675742 sequences (100000262 bp) in 1.699 CPU sec, 1.696 real sec...
[M::mem_process_seqs] Processed 674844 reads in 167.438 CPU sec, 5.312 real sec
[M::mem_process_seqs] Processed 676500 reads in 108.945 CPU sec, 3.446 real sec
sorting block 104, #reads = 1077043 mem = 536878138 peak = 1174269952
[M::process] read 675832 sequences (100000183 bp) in 2.615 CPU sec, 2.786 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285204, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 337, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (357.40, 164.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675742 reads in 92.081 CPU sec, 2.899 real sec
[M::process] read 675846 sequences (100000009 bp) in 1.991 CPU sec, 2.013 real sec...
sorting block 105, #reads = 1077228 mem = 536884058 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285698, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (354.57, 164.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285323, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (355.78, 163.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 304, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (255.27, 70.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676314 reads in 260.271 CPU sec, 8.279 real sec
[M::process] read 674936 sequences (100000075 bp) in 1.955 CPU sec, 1.987 real sec...
[M::mem_process_seqs] Processed 675832 reads in 127.909 CPU sec, 4.062 real sec
sorting block 106, #reads = 1077107 mem = 536878184 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285170, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 166, 747)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1927)
[M::mem_pestat] mean and std.dev: (337.00, 269.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2517)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 336, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (355.49, 163.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674462 sequences (100000040 bp) in 2.818 CPU sec, 3.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284819, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 343, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (363.23, 166.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675846 reads in 165.365 CPU sec, 5.266 real sec
[M::process] read 675970 sequences (100000024 bp) in 1.769 CPU sec, 1.785 real sec...
[M::mem_process_seqs] Processed 674936 reads in 108.990 CPU sec, 3.441 real sec
sorting block 107, #reads = 1078089 mem = 536883173 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284313, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 351, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (369.40, 168.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676126 sequences (100000012 bp) in 2.606 CPU sec, 2.799 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285035, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (354.73, 164.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675618 sequences (100000007 bp) in 1.900 CPU sec, 1.904 real sec...
[M::mem_process_seqs] Processed 674462 reads in 166.780 CPU sec, 5.297 real sec
sorting block 108, #reads = 1078252 mem = 536879854 peak = 1174269952
[M::mem_process_seqs] Processed 675970 reads in 110.279 CPU sec, 3.482 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285222, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 332, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (353.35, 163.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675666 sequences (100000079 bp) in 2.408 CPU sec, 2.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285165, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (358.84, 167.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676018 sequences (100000091 bp) in 1.887 CPU sec, 1.889 real sec...
[M::mem_process_seqs] Processed 676126 reads in 166.300 CPU sec, 5.292 real sec
sorting block 109, #reads = 1077269 mem = 536878497 peak = 1174269952
[M::mem_process_seqs] Processed 675618 reads in 106.770 CPU sec, 3.376 real sec
sorting block 110, #reads = 1078419 mem = 536881641 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285318, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (360.09, 168.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676026 sequences (100000022 bp) in 2.773 CPU sec, 2.927 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285595, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 335, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (358.54, 169.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675666 reads in 166.967 CPU sec, 5.306 real sec
[M::process] read 677174 sequences (100000096 bp) in 1.985 CPU sec, 2.004 real sec...
[M::mem_process_seqs] Processed 676018 reads in 108.486 CPU sec, 3.416 real sec
sorting block 111, #reads = 1077716 mem = 536879996 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 285561, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (343, 703, 703)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1423)
[M::mem_pestat] mean and std.dev: (525.25, 194.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1783)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (356.77, 167.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 677842 sequences (100000167 bp) in 2.176 CPU sec, 2.300 real sec...
[M::process] read 677380 sequences (100000027 bp) in 1.850 CPU sec, 1.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 285340, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (411, 585, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (63, 933)
[M::mem_pestat] mean and std.dev: (468.77, 132.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 324, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (347.86, 163.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676026 reads in 187.728 CPU sec, 5.991 real sec
[M::mem_process_seqs] Processed 677174 reads in 128.705 CPU sec, 4.089 real sec
sorting block 112, #reads = 1076501 mem = 536879634 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285876, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 319, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (339.26, 155.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677212 sequences (100000231 bp) in 2.805 CPU sec, 2.952 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286104, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 323, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (342.78, 157.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676868 sequences (100000149 bp) in 1.857 CPU sec, 1.861 real sec...
[M::mem_process_seqs] Processed 677842 reads in 165.121 CPU sec, 5.236 real sec
sorting block 113, #reads = 1069622 mem = 536878366 peak = 1174269952
[M::mem_process_seqs] Processed 677380 reads in 105.146 CPU sec, 3.319 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285916, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 324, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (343.58, 157.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 114, #reads = 1078527 mem = 536883177 peak = 1174269952
[M::process] read 676746 sequences (100000236 bp) in 2.329 CPU sec, 2.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285565, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (345.89, 158.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675132 sequences (100000031 bp) in 1.652 CPU sec, 1.655 real sec...
[M::mem_process_seqs] Processed 677212 reads in 160.881 CPU sec, 5.135 real sec
[M::mem_process_seqs] Processed 676868 reads in 105.135 CPU sec, 3.328 real sec
sorting block 115, #reads = 1079934 mem = 536880775 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285539, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (286, 314, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (230, 370)
[M::mem_pestat] mean and std.dev: (303.50, 13.56)
[M::mem_pestat] low and high boundaries for proper pairs: (202, 398)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (348.91, 160.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675688 sequences (100000171 bp) in 2.537 CPU sec, 2.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285269, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 342, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (357.14, 159.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676746 reads in 158.846 CPU sec, 5.055 real sec
[M::process] read 677562 sequences (100000223 bp) in 1.878 CPU sec, 1.902 real sec...
[M::mem_process_seqs] Processed 675132 reads in 101.344 CPU sec, 3.187 real sec
sorting block 116, #reads = 1079578 mem = 536878749 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285573, 1, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (244, 382, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (336.80, 122.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (353.95, 158.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 677766 sequences (100000225 bp) in 2.382 CPU sec, 2.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285817, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 319, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (338.16, 154.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677952 sequences (100000280 bp) in 1.946 CPU sec, 1.950 real sec...
[M::mem_process_seqs] Processed 675688 reads in 163.844 CPU sec, 5.206 real sec
[M::mem_process_seqs] Processed 677562 reads in 104.290 CPU sec, 3.290 real sec
sorting block 117, #reads = 1078662 mem = 536880309 peak = 1174269952
[M::process] read 677560 sequences (100000215 bp) in 2.686 CPU sec, 2.872 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286198, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 315, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (334.89, 152.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677952 reads in 92.877 CPU sec, 2.916 real sec
[M::process] read 677012 sequences (100000236 bp) in 1.960 CPU sec, 1.981 real sec...
sorting block 118, #reads = 1080371 mem = 536879431 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286020, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 316, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (334.58, 151.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1095)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286543, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 318, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (337.58, 153.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677766 reads in 255.701 CPU sec, 8.126 real sec
[M::mem_process_seqs] Processed 677560 reads in 122.689 CPU sec, 3.884 real sec
[M::process] read 677130 sequences (100000233 bp) in 2.353 CPU sec, 2.391 real sec...
sorting block 119, #reads = 1080349 mem = 536881885 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286173, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 322, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (341.56, 155.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675846 sequences (100000147 bp) in 2.679 CPU sec, 2.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285505, 4, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 149, 202)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (43, 308)
[M::mem_pestat] mean and std.dev: (155.18, 43.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 361)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 323, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (343.05, 157.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 677012 reads in 161.380 CPU sec, 5.150 real sec
sorting block 120, #reads = 1079205 mem = 536877764 peak = 1174269952
[M::process] read 675504 sequences (100000030 bp) in 2.121 CPU sec, 2.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285098, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 337, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (353.32, 159.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675846 reads in 91.217 CPU sec, 2.885 real sec
[M::process] read 677194 sequences (100000066 bp) in 2.013 CPU sec, 2.020 real sec...
[M::mem_process_seqs] Processed 677130 reads in 231.723 CPU sec, 7.336 real sec
sorting block 121, #reads = 1079065 mem = 536878112 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285455, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 339, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (355.86, 159.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676764 sequences (100000152 bp) in 2.470 CPU sec, 2.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286010, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 324, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (343.07, 156.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675504 reads in 175.676 CPU sec, 5.562 real sec
[M::mem_process_seqs] Processed 677194 reads in 104.969 CPU sec, 3.305 real sec
[M::process] read 676588 sequences (100000252 bp) in 2.132 CPU sec, 2.212 real sec...
sorting block 122, #reads = 1079976 mem = 536878807 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285356, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 325, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (343.74, 156.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676310 sequences (100000236 bp) in 2.367 CPU sec, 2.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285320, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (345.54, 157.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676764 reads in 166.632 CPU sec, 5.306 real sec
[M::process] read 676310 sequences (100000292 bp) in 1.878 CPU sec, 1.883 real sec...
sorting block 123, #reads = 1080718 mem = 536882953 peak = 1174269952
[M::mem_process_seqs] Processed 676588 reads in 105.894 CPU sec, 3.336 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284921, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (348.84, 158.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 124, #reads = 1078822 mem = 536880539 peak = 1174269952
[M::process] read 675948 sequences (100000074 bp) in 2.588 CPU sec, 2.766 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285407, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 334, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (353.31, 162.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676310 reads in 167.922 CPU sec, 5.332 real sec
[M::process] read 676200 sequences (100000046 bp) in 1.958 CPU sec, 1.964 real sec...
[M::mem_process_seqs] Processed 676310 reads in 111.587 CPU sec, 3.519 real sec
sorting block 125, #reads = 1078443 mem = 536879392 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284828, 5, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 332, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (351.56, 160.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (296, 357, 798)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1802)
[M::mem_pestat] mean and std.dev: (429.38, 224.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2304)
[M::mem_pestat] skip orientation RR
[M::process] read 676346 sequences (100000211 bp) in 2.453 CPU sec, 2.609 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285206, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (352.94, 162.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676424 sequences (100000190 bp) in 1.980 CPU sec, 1.983 real sec...
[M::mem_process_seqs] Processed 675948 reads in 168.398 CPU sec, 5.347 real sec
[M::mem_process_seqs] Processed 676200 reads in 112.750 CPU sec, 3.554 real sec
sorting block 126, #reads = 1078140 mem = 536879518 peak = 1174269952
[M::process] read 676322 sequences (100000072 bp) in 2.678 CPU sec, 2.844 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285430, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (352.77, 163.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676424 reads in 94.766 CPU sec, 2.968 real sec
[M::process] read 676664 sequences (100000011 bp) in 2.078 CPU sec, 2.096 real sec...
sorting block 127, #reads = 1076768 mem = 536879890 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285578, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (352.95, 163.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285166, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (353.25, 163.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676966 sequences (100000177 bp) in 1.778 CPU sec, 1.788 real sec...
[M::mem_process_seqs] Processed 676346 reads in 261.637 CPU sec, 8.312 real sec
[M::mem_process_seqs] Processed 676322 reads in 126.409 CPU sec, 4.028 real sec
sorting block 128, #reads = 1077623 mem = 536878638 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285355, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 325, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (348.78, 163.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (401, 1096, 1366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3296)
[M::mem_pestat] mean and std.dev: (827.80, 496.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4261)
[M::mem_pestat] skip orientation RR
[M::process] read 677974 sequences (100000153 bp) in 2.696 CPU sec, 2.859 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285785, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 319, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (344.09, 161.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679294 sequences (100000060 bp) in 1.884 CPU sec, 1.894 real sec...
[M::mem_process_seqs] Processed 676664 reads in 174.471 CPU sec, 5.544 real sec
[M::mem_process_seqs] Processed 676966 reads in 114.983 CPU sec, 3.633 real sec
sorting block 129, #reads = 1076877 mem = 536878958 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285638, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 317, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (340.37, 159.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678764 sequences (100000048 bp) in 2.809 CPU sec, 2.924 real sec...
[M::process] read 679290 sequences (100000249 bp) in 1.983 CPU sec, 1.989 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286878, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 314, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (331.81, 150.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677974 reads in 193.849 CPU sec, 6.154 real sec
sorting block 130, #reads = 1073047 mem = 536877612 peak = 1174269952
[M::mem_process_seqs] Processed 679294 reads in 123.716 CPU sec, 3.917 real sec
sorting block 131, #reads = 1070095 mem = 536880433 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285467, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 317, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (333.06, 149.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679374 sequences (100000119 bp) in 2.526 CPU sec, 2.672 real sec...
[M::process] read 676684 sequences (100000102 bp) in 1.942 CPU sec, 1.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286953, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 311, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (329.09, 149.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678764 reads in 185.546 CPU sec, 5.894 real sec
[M::mem_process_seqs] Processed 679290 reads in 121.426 CPU sec, 3.847 real sec
sorting block 132, #reads = 1071357 mem = 536879967 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286573, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 307, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (325.61, 147.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678338 sequences (100000243 bp) in 2.784 CPU sec, 2.933 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285498, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (349.08, 154.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678534 sequences (100000085 bp) in 1.850 CPU sec, 1.857 real sec...
[M::mem_process_seqs] Processed 679374 reads in 177.670 CPU sec, 5.641 real sec
[M::mem_process_seqs] Processed 676684 reads in 112.883 CPU sec, 3.571 real sec
sorting block 133, #reads = 1072255 mem = 536876790 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286397, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 319, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (336.66, 152.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679384 sequences (100000287 bp) in 2.513 CPU sec, 2.621 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286268, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 315, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (335.20, 153.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (298, 298, 643)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1333)
[M::mem_pestat] mean and std.dev: (343.40, 171.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1678)
[M::mem_pestat] skip orientation RR
[M::process] read 679538 sequences (100000162 bp) in 1.982 CPU sec, 1.983 real sec...
[M::mem_process_seqs] Processed 678338 reads in 176.544 CPU sec, 5.606 real sec
[M::mem_process_seqs] Processed 678534 reads in 112.122 CPU sec, 3.536 real sec
sorting block 134, #reads = 1073963 mem = 536880163 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286408, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 307, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (327.83, 150.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678582 sequences (100000216 bp) in 2.883 CPU sec, 3.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286253, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 303, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (322.61, 145.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678146 sequences (100000268 bp) in 1.905 CPU sec, 1.914 real sec...
[M::mem_process_seqs] Processed 679384 reads in 175.962 CPU sec, 5.589 real sec
sorting block 135, #reads = 1076529 mem = 536878408 peak = 1174269952
[M::mem_process_seqs] Processed 679538 reads in 115.957 CPU sec, 3.657 real sec
sorting block 136, #reads = 1076304 mem = 536880775 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 286612, 4, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (485, 859, 859)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1607)
[M::mem_pestat] mean and std.dev: (698.92, 252.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1981)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 311, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (328.73, 147.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1076)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676542 sequences (100000068 bp) in 2.451 CPU sec, 2.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285603, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 314, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (332.04, 149.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676788 sequences (100000063 bp) in 1.868 CPU sec, 1.877 real sec...
[M::mem_process_seqs] Processed 678582 reads in 172.228 CPU sec, 5.478 real sec
[M::mem_process_seqs] Processed 678146 reads in 110.192 CPU sec, 3.478 real sec
sorting block 137, #reads = 1076825 mem = 536880087 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285863, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (343.06, 151.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677696 sequences (100000046 bp) in 2.705 CPU sec, 2.832 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284844, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (341.95, 152.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676542 reads in 172.199 CPU sec, 5.469 real sec
[M::process] read 677586 sequences (100000105 bp) in 1.979 CPU sec, 1.988 real sec...
[M::mem_process_seqs] Processed 676788 reads in 110.413 CPU sec, 3.477 real sec
sorting block 138, #reads = 1076158 mem = 536878947 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285437, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 319, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (336.92, 151.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676626 sequences (100000009 bp) in 2.594 CPU sec, 2.760 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285123, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 316, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (334.49, 150.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677696 reads in 165.901 CPU sec, 5.278 real sec
[M::process] read 677198 sequences (100000119 bp) in 1.898 CPU sec, 1.913 real sec...
[M::mem_process_seqs] Processed 677586 reads in 107.342 CPU sec, 3.385 real sec
sorting block 139, #reads = 1076591 mem = 536883320 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285637, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 327, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (343.79, 153.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677008 sequences (100000011 bp) in 2.422 CPU sec, 2.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285689, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 321, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (339.50, 153.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676702 sequences (100000148 bp) in 1.758 CPU sec, 1.768 real sec...
[M::mem_process_seqs] Processed 676626 reads in 164.425 CPU sec, 5.244 real sec
sorting block 140, #reads = 1077385 mem = 536879021 peak = 1174269952
[M::mem_process_seqs] Processed 677198 reads in 103.561 CPU sec, 3.284 real sec
sorting block 141, #reads = 1077992 mem = 536878049 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285166, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 323, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (342.07, 154.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676652 sequences (100000147 bp) in 2.085 CPU sec, 2.214 real sec...
[M::process] read 676460 sequences (100000280 bp) in 1.400 CPU sec, 1.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285247, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (345.43, 156.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677008 reads in 168.509 CPU sec, 5.383 real sec
[M::mem_process_seqs] Processed 676702 reads in 107.146 CPU sec, 3.406 real sec
sorting block 142, #reads = 1078123 mem = 536879977 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285324, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (343.93, 155.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (404, 404, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (308, 548)
[M::mem_pestat] mean and std.dev: (428.71, 22.29)
[M::mem_pestat] low and high boundaries for proper pairs: (260, 596)
[M::mem_pestat] skip orientation RR
[M::process] read 676336 sequences (100000161 bp) in 2.813 CPU sec, 2.929 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285616, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (346.44, 157.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676598 sequences (100000118 bp) in 1.861 CPU sec, 1.861 real sec...
[M::mem_process_seqs] Processed 676652 reads in 170.795 CPU sec, 5.419 real sec
[M::mem_process_seqs] Processed 676460 reads in 108.974 CPU sec, 3.439 real sec
sorting block 143, #reads = 1077817 mem = 536877117 peak = 1174269952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284970, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (345.90, 157.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676304 sequences (100000172 bp) in 2.384 CPU sec, 2.506 real sec...
[M::process] read 676372 sequences (100000156 bp) in 1.632 CPU sec, 1.635 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285420, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (347.70, 159.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676336 reads in 170.447 CPU sec, 5.426 real sec
sorting block 144, #reads = 1077835 mem = 536879189 peak = 1174269952
[M::mem_process_seqs] Processed 676598 reads in 109.211 CPU sec, 3.461 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285324, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (349.68, 161.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 408030 sequences (60237389 bp) in 1.901 CPU sec, 1.999 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285163, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (349.75, 161.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676304 reads in 163.435 CPU sec, 5.269 real sec
sorting block 145, #reads = 1078373 mem = 536877640 peak = 1174269952
[M::mem_process_seqs] Processed 676372 reads in 106.032 CPU sec, 3.469 real sec
sorting block 146, #reads = 1078044 mem = 536877711 peak = 1192656896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 171700, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 320, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (343.63, 160.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 408030 reads in 58.498 CPU sec, 1.953 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 158578324
overall: 69901168640 mem 22150.323 user 501.490 sys 744.402 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_d0M-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:659_d0M-T1-TDNA-1_A23MHWWLT4_1\tLB:659_d0M-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_d0M-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_d0M-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 744.446 sec; CPU: 22651.816 sec
sorting block 147, #reads = 686615 mem = 342215798 peak = 1192656896
uniq header count 1
merging 148 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 128
algo: util-sort
license: sentieon:util=1
output file size: 8415812764
output reads: 159352889
bam_mem_sort: 148 calls 84.962 user 1.497 sys 87.006 real
bam_write: 148 calls 68.912 user 2.968 sys 82.225 real
execute: 1 calls 42.459 user 1.619 sys 800.740 real
merge_files: 1 calls 42.433 user 1.597 sys 55.866 real
parse_chunk: 2363 calls 99.993 user 25.913 sys 130.727 real
read_chunk: 6364 calls 51.558 user 34.111 sys 687.091 real
sort_block: 1 calls 32.139 user 33.490 sys 660.609 real
write_chunk: 3999 calls 41.787 user 1.193 sys 54.449 real
overall: 3320430592 mem 2152.797 user 86.727 sys 800.761 real
ls: cannot access '*.cram': No such file or directory