cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1592.246 CPU sec, 476.439 real sec
[M::process] read 702456 sequences (100000004 bp) in 0.963 CPU sec, 0.965 real sec...
[M::process] read 701098 sequences (100000117 bp) in 1.239 CPU sec, 1.241 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292252, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (238.92, 112.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700222 sequences (100000134 bp) in 1.189 CPU sec, 1.197 real sec...
[M::process] read 700046 sequences (100000153 bp) in 1.170 CPU sec, 1.168 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291393, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (243.83, 115.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702456 reads in 144.674 CPU sec, 4.565 real sec
[M::mem_process_seqs] Processed 701098 reads in 80.113 CPU sec, 2.556 real sec
sorting block 0, #reads = 1096468 mem = 536878694 peak = 603414528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291700, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.03, 117.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699450 sequences (100000155 bp) in 1.574 CPU sec, 1.630 real sec...
[M::process] read 698706 sequences (100000100 bp) in 1.240 CPU sec, 1.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 291380, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 213, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (216.14, 97.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (246.75, 117.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700222 reads in 122.789 CPU sec, 3.901 real sec
[M::mem_process_seqs] Processed 700046 reads in 75.949 CPU sec, 2.426 real sec
sorting block 1, #reads = 1095986 mem = 536876666 peak = 724172800
[M::process] read 698184 sequences (100000011 bp) in 1.459 CPU sec, 1.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291033, 8, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 265, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (223.20, 87.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (248.56, 119.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698204 sequences (100000079 bp) in 1.215 CPU sec, 1.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290977, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.75, 120.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699450 reads in 121.811 CPU sec, 3.872 real sec
[M::mem_process_seqs] Processed 698706 reads in 76.481 CPU sec, 2.441 real sec
sorting block 2, #reads = 1094702 mem = 536877845 peak = 850501632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290845, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (251.02, 120.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698104 sequences (100000209 bp) in 1.917 CPU sec, 2.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290781, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.94, 120.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698118 sequences (100000252 bp) in 1.340 CPU sec, 1.340 real sec...
[M::mem_process_seqs] Processed 698184 reads in 121.856 CPU sec, 3.862 real sec
sorting block 3, #reads = 1094593 mem = 536878021 peak = 850501632
[M::mem_process_seqs] Processed 698204 reads in 77.239 CPU sec, 2.439 real sec
sorting block 4, #reads = 1093439 mem = 536880135 peak = 850501632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 291064, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.13, 119.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697580 sequences (100000112 bp) in 1.712 CPU sec, 1.801 real sec...
[M::process] read 697632 sequences (100000123 bp) in 1.260 CPU sec, 1.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291095, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 244, 639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1569)
[M::mem_pestat] mean and std.dev: (270.22, 180.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2034)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.44, 118.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698104 reads in 123.701 CPU sec, 3.933 real sec
[M::mem_process_seqs] Processed 698118 reads in 78.054 CPU sec, 2.474 real sec
sorting block 5, #reads = 1093558 mem = 536882990 peak = 850501632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290826, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.34, 118.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697400 sequences (100000062 bp) in 2.009 CPU sec, 2.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290937, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.72, 119.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696436 sequences (100000156 bp) in 1.430 CPU sec, 1.430 real sec...
[M::mem_process_seqs] Processed 697580 reads in 123.779 CPU sec, 3.925 real sec
[M::mem_process_seqs] Processed 697632 reads in 77.955 CPU sec, 2.461 real sec
sorting block 6, #reads = 1092789 mem = 536878809 peak = 850501632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290397, 8, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.75, 118.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (8, 563)
[M::mem_pestat] mean and std.dev: (274.45, 70.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 674)
[M::mem_pestat] skip orientation RR
[M::process] read 696250 sequences (100000236 bp) in 2.066 CPU sec, 2.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290539, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 837, 2818)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8098)
[M::mem_pestat] mean and std.dev: (1181.00, 1132.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10738)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.81, 120.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697400 reads in 124.002 CPU sec, 3.951 real sec
[M::mem_process_seqs] Processed 696436 reads in 78.622 CPU sec, 2.478 real sec
sorting block 7, #reads = 1093387 mem = 536884187 peak = 850501632
[M::process] read 695996 sequences (100000151 bp) in 1.868 CPU sec, 1.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290198, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 384, 831)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081)
[M::mem_pestat] mean and std.dev: (501.90, 445.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2706)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (253.16, 119.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 695484 sequences (100000003 bp) in 2.256 CPU sec, 2.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290380, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (253.53, 118.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696250 reads in 126.494 CPU sec, 4.028 real sec
sorting block 8, #reads = 1092810 mem = 536877128 peak = 850501632
[M::mem_process_seqs] Processed 695996 reads in 80.457 CPU sec, 2.539 real sec
[M::process] read 695086 sequences (100000252 bp) in 2.058 CPU sec, 2.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290082, 7, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (103, 231, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1327)
[M::mem_pestat] mean and std.dev: (285.22, 222.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1735)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (255.14, 119.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 9, #reads = 1092124 mem = 536876825 peak = 850501632
[M::process] read 698590 sequences (100000183 bp) in 2.075 CPU sec, 2.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290204, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 234, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (257.09, 121.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695484 reads in 126.665 CPU sec, 4.042 real sec
[M::mem_process_seqs] Processed 695086 reads in 80.438 CPU sec, 2.544 real sec
[M::process] read 701602 sequences (100000145 bp) in 2.432 CPU sec, 2.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291174, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.69, 117.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 10, #reads = 1092023 mem = 536879076 peak = 850501632
[M::process] read 701116 sequences (100000182 bp) in 2.013 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291923, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (242.80, 115.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698590 reads in 130.984 CPU sec, 4.208 real sec
[M::mem_process_seqs] Processed 701602 reads in 83.038 CPU sec, 2.634 real sec
sorting block 11, #reads = 1092171 mem = 536877667 peak = 850501632
[M::process] read 700180 sequences (100000253 bp) in 2.415 CPU sec, 2.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291789, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.16, 117.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 291449, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 335, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (237.50, 125.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1343)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 699288 sequences (100000109 bp) in 1.915 CPU sec, 1.988 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.50, 119.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 242, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (212, 287)
[M::mem_pestat] mean and std.dev: (251.82, 9.73)
[M::mem_pestat] low and high boundaries for proper pairs: (197, 302)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701116 reads in 129.709 CPU sec, 4.145 real sec
[M::mem_process_seqs] Processed 700180 reads in 82.313 CPU sec, 2.607 real sec
sorting block 12, #reads = 1097148 mem = 536877692 peak = 850501632
[M::process] read 699160 sequences (100000068 bp) in 1.898 CPU sec, 1.997 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291343, 7, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 481, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186)
[M::mem_pestat] mean and std.dev: (361.25, 164.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1518)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.23, 119.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 13, #reads = 1095346 mem = 536880431 peak = 878694400
[M::process] read 698574 sequences (100000060 bp) in 1.783 CPU sec, 1.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291154, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (249.87, 120.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699288 reads in 129.440 CPU sec, 4.120 real sec
[M::mem_process_seqs] Processed 699160 reads in 82.087 CPU sec, 2.590 real sec
[M::process] read 698010 sequences (100000291 bp) in 1.510 CPU sec, 1.541 real sec...
sorting block 14, #reads = 1095591 mem = 536884239 peak = 878694400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290712, 10, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.20, 120.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 205, 626)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1618)
[M::mem_pestat] mean and std.dev: (355.00, 322.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2114)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 697890 sequences (100000196 bp) in 1.904 CPU sec, 2.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290555, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.10, 121.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698574 reads in 126.668 CPU sec, 4.035 real sec
[M::process] read 698092 sequences (100000155 bp) in 1.481 CPU sec, 1.494 real sec...
[M::mem_process_seqs] Processed 698010 reads in 80.607 CPU sec, 2.542 real sec
sorting block 15, #reads = 1094354 mem = 536877323 peak = 878694400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 291033, 9, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 411, 3697)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10789)
[M::mem_pestat] mean and std.dev: (1172.59, 1655.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14335)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (251.82, 121.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698068 sequences (100000050 bp) in 2.056 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290890, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.02, 119.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 280, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739)
[M::mem_pestat] mean and std.dev: (263.10, 91.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 908)
[M::mem_pestat] skip orientation RR
[M::process] read 698252 sequences (100000065 bp) in 1.330 CPU sec, 1.334 real sec...
[M::mem_process_seqs] Processed 697890 reads in 128.547 CPU sec, 4.090 real sec
[M::mem_process_seqs] Processed 698092 reads in 81.264 CPU sec, 2.572 real sec
sorting block 16, #reads = 1093438 mem = 536878219 peak = 878694400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290413, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.40, 119.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697670 sequences (100000010 bp) in 1.813 CPU sec, 1.906 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290681, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.73, 118.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697752 sequences (100000189 bp) in 1.360 CPU sec, 1.358 real sec...
[M::mem_process_seqs] Processed 698068 reads in 124.690 CPU sec, 3.965 real sec
sorting block 17, #reads = 1093567 mem = 536880220 peak = 894144512
[M::mem_process_seqs] Processed 698252 reads in 78.448 CPU sec, 2.480 real sec
sorting block 18, #reads = 1093279 mem = 536880573 peak = 931254272
[M::process] read 697482 sequences (100000292 bp) in 1.933 CPU sec, 2.032 real sec...
[M::process] read 696780 sequences (100000236 bp) in 1.380 CPU sec, 1.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290393, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.57, 120.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697482 reads in 65.019 CPU sec, 2.256 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290662, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.00, 118.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697752 reads in 137.328 CPU sec, 4.935 real sec
sorting block 19, #reads = 1094312 mem = 536877689 peak = 931254272
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290693, 14, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (327, 374, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (193, 528)
[M::mem_pestat] mean and std.dev: (351.25, 52.28)
[M::mem_pestat] low and high boundaries for proper pairs: (126, 595)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.02, 118.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (89, 200, 834)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2324)
[M::mem_pestat] mean and std.dev: (520.79, 572.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3069)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 696530 sequences (100000288 bp) in 2.082 CPU sec, 2.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290113, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.40, 119.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (111, 232, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (198.60, 82.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 875)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697670 reads in 258.129 CPU sec, 8.787 real sec
[M::mem_process_seqs] Processed 696780 reads in 92.158 CPU sec, 2.920 real sec
sorting block 20, #reads = 1093814 mem = 536877481 peak = 931254272
[M::process] read 695950 sequences (100000291 bp) in 1.925 CPU sec, 2.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290593, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 207, 6963)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20587)
[M::mem_pestat] mean and std.dev: (1885.67, 2932.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 27399)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.73, 119.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696078 sequences (100000048 bp) in 1.740 CPU sec, 1.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290120, 9, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (254.21, 120.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696530 reads in 123.995 CPU sec, 3.948 real sec
[M::mem_process_seqs] Processed 695950 reads in 78.980 CPU sec, 2.492 real sec
sorting block 21, #reads = 1092410 mem = 536879097 peak = 931254272
[M::process] read 702328 sequences (100000212 bp) in 1.772 CPU sec, 1.836 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 289923, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (255.89, 121.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 298, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (214, 354)
[M::mem_pestat] mean and std.dev: (284.57, 12.12)
[M::mem_pestat] low and high boundaries for proper pairs: (186, 382)
[M::mem_pestat] skip orientation RR
sorting block 22, #reads = 1092629 mem = 536877744 peak = 938852352
[M::process] read 701004 sequences (100000250 bp) in 2.019 CPU sec, 2.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292497, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.01, 114.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696078 reads in 126.355 CPU sec, 4.023 real sec
[M::mem_process_seqs] Processed 702328 reads in 81.355 CPU sec, 2.566 real sec
[M::process] read 700558 sequences (100000222 bp) in 1.576 CPU sec, 1.619 real sec...
sorting block 23, #reads = 1093058 mem = 536879169 peak = 938852352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291704, 8, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (115, 173, 1014)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2812)
[M::mem_pestat] mean and std.dev: (492.91, 532.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3711)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (243.68, 115.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699634 sequences (100000267 bp) in 1.816 CPU sec, 1.911 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291452, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.75, 117.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701004 reads in 124.963 CPU sec, 3.980 real sec
[M::process] read 698656 sequences (100000167 bp) in 1.375 CPU sec, 1.379 real sec...
[M::mem_process_seqs] Processed 700558 reads in 79.553 CPU sec, 2.508 real sec
sorting block 24, #reads = 1096053 mem = 536878589 peak = 938852352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291674, 7, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.65, 117.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 287, 650)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1586)
[M::mem_pestat] mean and std.dev: (364.38, 199.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2054)
[M::mem_pestat] skip orientation RR
[M::process] read 698632 sequences (100000116 bp) in 2.140 CPU sec, 2.243 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291168, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.68, 118.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699634 reads in 123.788 CPU sec, 3.928 real sec
[M::process] read 697812 sequences (100000156 bp) in 1.389 CPU sec, 1.383 real sec...
[M::mem_process_seqs] Processed 698656 reads in 79.099 CPU sec, 2.495 real sec
sorting block 25, #reads = 1095195 mem = 536880030 peak = 938852352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291513, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.24, 119.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697744 sequences (100000216 bp) in 2.156 CPU sec, 2.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290819, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 237, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497)
[M::mem_pestat] mean and std.dev: (214.25, 44.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 603)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (252.86, 121.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 246, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521)
[M::mem_pestat] mean and std.dev: (210.25, 64.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 639)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698632 reads in 123.198 CPU sec, 3.913 real sec
sorting block 26, #reads = 1093618 mem = 536882132 peak = 938852352
[M::process] read 697530 sequences (100000047 bp) in 1.460 CPU sec, 1.469 real sec...
[M::mem_process_seqs] Processed 697812 reads in 78.613 CPU sec, 2.481 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291143, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.31, 121.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 27, #reads = 1094274 mem = 536878624 peak = 938852352
[M::process] read 697606 sequences (100000160 bp) in 1.910 CPU sec, 2.004 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290780, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.09, 120.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 219, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1061)
[M::mem_pestat] mean and std.dev: (203.00, 104.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1371)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697744 reads in 124.654 CPU sec, 3.965 real sec
[M::process] read 697560 sequences (100000053 bp) in 1.416 CPU sec, 1.422 real sec...
[M::mem_process_seqs] Processed 697530 reads in 79.837 CPU sec, 2.517 real sec
sorting block 28, #reads = 1094032 mem = 536877859 peak = 938852352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290616, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.21, 119.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 262, 622)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1398)
[M::mem_pestat] mean and std.dev: (346.30, 203.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1786)
[M::mem_pestat] skip orientation RR
[M::process] read 697696 sequences (100000275 bp) in 2.003 CPU sec, 2.132 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290581, 5, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 195, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (179.10, 57.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.26, 119.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 281, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (292.42, 89.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 950)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697606 reads in 124.448 CPU sec, 3.966 real sec
[M::mem_process_seqs] Processed 697560 reads in 79.359 CPU sec, 2.500 real sec
[M::process] read 697510 sequences (100000025 bp) in 1.560 CPU sec, 1.583 real sec...
sorting block 29, #reads = 1093384 mem = 536879193 peak = 938852352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290331, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (249.87, 118.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697380 sequences (100000265 bp) in 1.900 CPU sec, 2.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290350, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.27, 119.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697696 reads in 124.233 CPU sec, 3.953 real sec
[M::process] read 697248 sequences (100000048 bp) in 1.432 CPU sec, 1.445 real sec...
[M::mem_process_seqs] Processed 697510 reads in 78.662 CPU sec, 2.480 real sec
sorting block 30, #reads = 1093206 mem = 536879891 peak = 938852352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291077, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.88, 120.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1092988 mem = 536877342 peak = 982458368
[M::process] read 696648 sequences (100000005 bp) in 1.955 CPU sec, 2.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290195, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 195, 658)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1680)
[M::mem_pestat] mean and std.dev: (291.00, 213.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2191)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (252.15, 120.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697380 reads in 123.358 CPU sec, 3.929 real sec
[M::process] read 696136 sequences (100000076 bp) in 1.500 CPU sec, 1.522 real sec...
[M::mem_process_seqs] Processed 697248 reads in 78.765 CPU sec, 2.482 real sec
sorting block 32, #reads = 1094070 mem = 536879724 peak = 982458368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290345, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.20, 120.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695576 sequences (100000300 bp) in 2.226 CPU sec, 2.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290053, 8, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.55, 121.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 191, 198)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (51, 296)
[M::mem_pestat] mean and std.dev: (164.75, 32.72)
[M::mem_pestat] low and high boundaries for proper pairs: (2, 345)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696648 reads in 123.649 CPU sec, 3.932 real sec
[M::process] read 700182 sequences (100000248 bp) in 1.577 CPU sec, 1.606 real sec...
sorting block 33, #reads = 1092963 mem = 536880875 peak = 982458368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290243, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (256.56, 122.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695576 reads in 68.249 CPU sec, 2.164 real sec
[M::process] read 702018 sequences (100000070 bp) in 1.501 CPU sec, 1.515 real sec...
[M::mem_process_seqs] Processed 696136 reads in 168.371 CPU sec, 5.335 real sec
sorting block 34, #reads = 1092353 mem = 536877988 peak = 982458368
[M::process] read 701450 sequences (100000243 bp) in 2.210 CPU sec, 2.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291539, 9, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.67, 116.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702018 reads in 68.197 CPU sec, 2.141 real sec
[M::process] read 700676 sequences (100000165 bp) in 1.719 CPU sec, 1.799 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291584, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.76, 117.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292215, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.36, 117.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (339, 1309, 1309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3249)
[M::mem_pestat] mean and std.dev: (845.20, 555.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4219)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 700182 reads in 201.592 CPU sec, 6.391 real sec
sorting block 35, #reads = 1094550 mem = 536878520 peak = 982458368
[M::mem_process_seqs] Processed 701450 reads in 90.094 CPU sec, 2.858 real sec
[M::process] read 699870 sequences (100000262 bp) in 1.767 CPU sec, 1.884 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291540, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.24, 118.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 287, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1438)
[M::mem_pestat] mean and std.dev: (358.36, 230.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1855)
[M::mem_pestat] skip orientation RR
sorting block 36, #reads = 1095187 mem = 536880126 peak = 982458368
[M::process] read 699536 sequences (100000126 bp) in 2.095 CPU sec, 2.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291240, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 317, 645)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1515)
[M::mem_pestat] mean and std.dev: (441.58, 351.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1950)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.08, 119.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700676 reads in 121.963 CPU sec, 3.886 real sec
[M::process] read 699870 sequences (100000061 bp) in 1.746 CPU sec, 1.818 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291173, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.13, 119.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699536 reads in 67.233 CPU sec, 2.119 real sec
[M::process] read 698480 sequences (100000239 bp) in 1.604 CPU sec, 1.623 real sec...
sorting block 37, #reads = 1096421 mem = 536880502 peak = 982458368
[M::mem_process_seqs] Processed 699870 reads in 170.452 CPU sec, 5.383 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291439, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.56, 119.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1095725 mem = 536879156 peak = 1014067200
[M::process] read 698146 sequences (100000032 bp) in 2.305 CPU sec, 2.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291272, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.78, 121.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699870 reads in 133.166 CPU sec, 4.224 real sec
[M::mem_process_seqs] Processed 698480 reads in 78.199 CPU sec, 2.472 real sec
[M::process] read 697762 sequences (100000224 bp) in 1.911 CPU sec, 2.051 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290454, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (251.04, 120.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 39, #reads = 1094779 mem = 536880951 peak = 1014067200
[M::process] read 697958 sequences (100000219 bp) in 1.847 CPU sec, 1.945 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290206, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.70, 120.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 218, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (199.60, 72.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1038)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698146 reads in 123.147 CPU sec, 3.922 real sec
[M::mem_process_seqs] Processed 697762 reads in 78.912 CPU sec, 2.489 real sec
[M::process] read 697860 sequences (100000019 bp) in 1.732 CPU sec, 1.781 real sec...
sorting block 40, #reads = 1093449 mem = 536880414 peak = 1014067200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290475, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.15, 119.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 155, 213)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387)
[M::mem_pestat] mean and std.dev: (147.33, 39.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 474)
[M::mem_pestat] skip orientation RR
[M::process] read 697568 sequences (100000114 bp) in 2.067 CPU sec, 2.184 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291190, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.37, 119.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (385, 1783, 2269)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6037)
[M::mem_pestat] mean and std.dev: (1456.00, 784.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7921)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697958 reads in 123.029 CPU sec, 3.913 real sec
[M::mem_process_seqs] Processed 697860 reads in 78.306 CPU sec, 2.465 real sec
[M::process] read 697514 sequences (100000254 bp) in 1.608 CPU sec, 1.645 real sec...
sorting block 41, #reads = 1092685 mem = 536878937 peak = 1014067200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290577, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.03, 118.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696608 sequences (100000043 bp) in 2.063 CPU sec, 2.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290576, 11, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.48, 118.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 249, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247)
[M::mem_pestat] mean and std.dev: (269.33, 161.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1599)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 221, 606)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1402)
[M::mem_pestat] mean and std.dev: (349.40, 323.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1800)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697568 reads in 123.234 CPU sec, 3.916 real sec
sorting block 42, #reads = 1093847 mem = 536878703 peak = 1014067200
[M::process] read 696202 sequences (100000001 bp) in 1.462 CPU sec, 1.478 real sec...
[M::mem_process_seqs] Processed 697514 reads in 79.163 CPU sec, 2.497 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290179, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 43, #reads = 1092655 mem = 536879361 peak = 1085186048
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.19, 119.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696222 sequences (100000125 bp) in 1.920 CPU sec, 2.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290283, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (252.19, 118.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696608 reads in 123.136 CPU sec, 3.920 real sec
[M::process] read 695740 sequences (100000074 bp) in 1.437 CPU sec, 1.453 real sec...
[M::mem_process_seqs] Processed 696202 reads in 78.448 CPU sec, 2.475 real sec
sorting block 44, #reads = 1092462 mem = 536877372 peak = 1085186048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290581, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.20, 119.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699734 sequences (100000215 bp) in 2.205 CPU sec, 2.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289864, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (255.95, 121.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696222 reads in 121.505 CPU sec, 3.865 real sec
[M::mem_process_seqs] Processed 695740 reads in 77.841 CPU sec, 2.450 real sec
[M::process] read 706278 sequences (100000234 bp) in 1.650 CPU sec, 1.684 real sec...
sorting block 45, #reads = 1091561 mem = 536881124 peak = 1085186048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291213, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (246.81, 118.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704794 sequences (100000091 bp) in 2.132 CPU sec, 2.229 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293401, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 202, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (192.11, 106.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 748)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 206, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (229.44, 109.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699734 reads in 126.943 CPU sec, 4.033 real sec
[M::process] read 703616 sequences (100000010 bp) in 1.487 CPU sec, 1.505 real sec...
sorting block 46, #reads = 1091490 mem = 536878616 peak = 1085186048
[M::mem_process_seqs] Processed 706278 reads in 80.734 CPU sec, 2.543 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293038, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (234.95, 113.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 189, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1134)
[M::mem_pestat] mean and std.dev: (290.90, 252.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1470)
[M::mem_pestat] skip orientation RR
sorting block 47, #reads = 1095286 mem = 536879892 peak = 1085186048
[M::process] read 702386 sequences (100000291 bp) in 2.282 CPU sec, 2.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 292885, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 281, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (229.55, 102.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (237.42, 114.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 704794 reads in 126.652 CPU sec, 4.022 real sec
[M::process] read 701996 sequences (100000280 bp) in 1.525 CPU sec, 1.540 real sec...
[M::mem_process_seqs] Processed 703616 reads in 80.657 CPU sec, 2.541 real sec
sorting block 48, #reads = 1097616 mem = 536878012 peak = 1085186048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291841, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.46, 116.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701086 sequences (100000246 bp) in 2.289 CPU sec, 2.416 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291989, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.06, 118.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702386 reads in 129.971 CPU sec, 4.129 real sec
[M::process] read 700838 sequences (100000202 bp) in 1.461 CPU sec, 1.470 real sec...
[M::mem_process_seqs] Processed 701996 reads in 80.261 CPU sec, 2.532 real sec
sorting block 49, #reads = 1094505 mem = 536879585 peak = 1085186048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292034, 12, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (325, 325, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (149, 589)
[M::mem_pestat] mean and std.dev: (346.25, 48.25)
[M::mem_pestat] low and high boundaries for proper pairs: (61, 677)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.76, 119.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (260, 260, 809)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1907)
[M::mem_pestat] mean and std.dev: (507.58, 469.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2456)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 699432 sequences (100000048 bp) in 2.270 CPU sec, 2.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291610, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 296, 1169)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3101)
[M::mem_pestat] mean and std.dev: (327.00, 291.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4067)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.74, 120.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701086 reads in 125.280 CPU sec, 3.981 real sec
[M::mem_process_seqs] Processed 700838 reads in 80.623 CPU sec, 2.534 real sec
[M::process] read 698890 sequences (100000219 bp) in 1.589 CPU sec, 1.611 real sec...
sorting block 50, #reads = 1095254 mem = 536877784 peak = 1085186048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291410, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 295, 1467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3955)
[M::mem_pestat] mean and std.dev: (634.92, 600.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5199)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.69, 121.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698690 sequences (100000287 bp) in 2.121 CPU sec, 2.254 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291002, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.97, 121.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 217, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (220.88, 73.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699432 reads in 130.821 CPU sec, 4.161 real sec
sorting block 51, #reads = 1094875 mem = 536880387 peak = 1085186048
[M::mem_process_seqs] Processed 698890 reads in 85.437 CPU sec, 2.690 real sec
[M::process] read 698498 sequences (100000063 bp) in 1.701 CPU sec, 1.731 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290651, 3, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 184, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420)
[M::mem_pestat] mean and std.dev: (193.60, 80.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 518)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.45, 120.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 52, #reads = 1092149 mem = 536881085 peak = 1085186048
[M::process] read 698646 sequences (100000113 bp) in 2.458 CPU sec, 2.561 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291048, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.62, 120.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698690 reads in 139.731 CPU sec, 4.458 real sec
[M::mem_process_seqs] Processed 698498 reads in 89.526 CPU sec, 2.819 real sec
[M::process] read 698008 sequences (100000087 bp) in 2.222 CPU sec, 2.310 real sec...
sorting block 53, #reads = 1092596 mem = 536884555 peak = 1085186048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291132, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.97, 119.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698082 sequences (100000226 bp) in 2.467 CPU sec, 2.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290739, 6, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (108, 286, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1350)
[M::mem_pestat] mean and std.dev: (249.38, 168.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1764)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.83, 120.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698646 reads in 140.365 CPU sec, 4.468 real sec
[M::mem_process_seqs] Processed 698008 reads in 89.016 CPU sec, 2.815 real sec
sorting block 54, #reads = 1093142 mem = 536879200 peak = 1085186048
[M::process] read 697592 sequences (100000065 bp) in 2.619 CPU sec, 2.739 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290857, 6, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.33, 119.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 193, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210)
[M::mem_pestat] mean and std.dev: (218.27, 128.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1564)
[M::mem_pestat] skip orientation RR
[M::process] read 697648 sequences (100000024 bp) in 2.326 CPU sec, 2.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290873, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.36, 118.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698082 reads in 136.366 CPU sec, 4.356 real sec
[M::mem_process_seqs] Processed 697592 reads in 86.870 CPU sec, 2.744 real sec
sorting block 55, #reads = 1091291 mem = 536879328 peak = 1085186048
[M::process] read 697536 sequences (100000076 bp) in 2.188 CPU sec, 2.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290551, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.38, 119.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1091281 mem = 536877626 peak = 1085186048
[M::process] read 707378 sequences (100000109 bp) in 2.000 CPU sec, 2.088 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290799, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.80, 120.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 608, 743)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1793)
[M::mem_pestat] mean and std.dev: (450.70, 258.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2318)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 697648 reads in 137.727 CPU sec, 4.383 real sec
[M::mem_process_seqs] Processed 697536 reads in 88.364 CPU sec, 2.780 real sec
[M::process] read 706262 sequences (100000246 bp) in 1.746 CPU sec, 1.806 real sec...
sorting block 57, #reads = 1091478 mem = 536880335 peak = 1085186048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293722, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 203, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582)
[M::mem_pestat] mean and std.dev: (226.67, 107.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 727)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704426 sequences (100000249 bp) in 2.244 CPU sec, 2.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293825, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (230.53, 109.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 748)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 707378 reads in 144.696 CPU sec, 4.602 real sec
[M::process] read 704416 sequences (100000014 bp) in 1.509 CPU sec, 1.527 real sec...
[M::mem_process_seqs] Processed 706262 reads in 91.130 CPU sec, 2.873 real sec
sorting block 58, #reads = 1092204 mem = 536885606 peak = 1085186048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293156, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (234.29, 111.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704230 sequences (100000099 bp) in 2.461 CPU sec, 2.553 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292574, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 260, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (250.00, 88.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (235.04, 112.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702778 sequences (100000023 bp) in 1.430 CPU sec, 1.422 real sec...
[M::mem_process_seqs] Processed 704426 reads in 139.673 CPU sec, 4.434 real sec
[M::mem_process_seqs] Processed 704416 reads in 89.054 CPU sec, 2.812 real sec
sorting block 59, #reads = 1094111 mem = 536879176 peak = 1085186048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293510, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (235.10, 112.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1093600 mem = 536878821 peak = 1085186048
[M::process] read 702300 sequences (100000250 bp) in 2.429 CPU sec, 2.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292696, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.63, 115.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701570 sequences (100000016 bp) in 1.500 CPU sec, 1.502 real sec...
[M::mem_process_seqs] Processed 704230 reads in 141.297 CPU sec, 4.482 real sec
[M::mem_process_seqs] Processed 702778 reads in 87.323 CPU sec, 2.758 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292563, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.36, 116.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 61, #reads = 1091962 mem = 536880635 peak = 1085186048
[M::process] read 700442 sequences (100000090 bp) in 2.447 CPU sec, 2.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292168, 5, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 200, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (254.82, 140.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1316)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (243.78, 118.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 702300 reads in 137.183 CPU sec, 4.353 real sec
[M::process] read 699422 sequences (100000041 bp) in 1.460 CPU sec, 1.462 real sec...
[M::mem_process_seqs] Processed 701570 reads in 87.344 CPU sec, 2.758 real sec
sorting block 62, #reads = 1093116 mem = 536879908 peak = 1085186048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291588, 1, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 254, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533)
[M::mem_pestat] mean and std.dev: (225.80, 63.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 661)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.03, 120.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699776 sequences (100000228 bp) in 2.663 CPU sec, 2.854 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291162, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.27, 120.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700442 reads in 138.154 CPU sec, 4.388 real sec
[M::mem_process_seqs] Processed 699422 reads in 87.806 CPU sec, 2.770 real sec
[M::process] read 698712 sequences (100000062 bp) in 1.606 CPU sec, 1.636 real sec...
sorting block 63, #reads = 1092148 mem = 536879603 peak = 1085186048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291365, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (246.88, 119.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698758 sequences (100000154 bp) in 2.092 CPU sec, 2.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291109, 6, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.15, 120.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 188, 1550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4296)
[M::mem_pestat] mean and std.dev: (552.23, 690.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5669)
[M::mem_pestat] skip orientation RR
[M::process] read 698450 sequences (100000298 bp) in 1.440 CPU sec, 1.436 real sec...
[M::mem_process_seqs] Processed 699776 reads in 137.814 CPU sec, 4.380 real sec
sorting block 64, #reads = 1092140 mem = 536879128 peak = 1085186048
[M::mem_process_seqs] Processed 698712 reads in 86.650 CPU sec, 2.734 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291061, 5, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.14, 120.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 259, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247)
[M::mem_pestat] mean and std.dev: (312.00, 188.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1603)
[M::mem_pestat] skip orientation RR
sorting block 65, #reads = 1091875 mem = 536879190 peak = 1087352832
[M::process] read 698100 sequences (100000122 bp) in 2.284 CPU sec, 2.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291047, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.93, 121.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698758 reads in 132.702 CPU sec, 4.219 real sec
[M::mem_process_seqs] Processed 698450 reads in 82.717 CPU sec, 2.604 real sec
[M::process] read 698178 sequences (100000225 bp) in 2.002 CPU sec, 2.040 real sec...
sorting block 66, #reads = 1091939 mem = 536881424 peak = 1087352832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291219, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.39, 121.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 817)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2067)
[M::mem_pestat] mean and std.dev: (395.00, 283.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2692)
[M::mem_pestat] skip orientation RR
[M::process] read 701432 sequences (100000106 bp) in 2.356 CPU sec, 2.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290634, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (251.15, 122.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698100 reads in 135.813 CPU sec, 4.321 real sec
[M::mem_process_seqs] Processed 698178 reads in 88.339 CPU sec, 2.779 real sec
[M::process] read 705248 sequences (100000090 bp) in 1.611 CPU sec, 1.636 real sec...
sorting block 67, #reads = 1091775 mem = 536877895 peak = 1087352832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291453, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (241.96, 117.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703446 sequences (100000262 bp) in 2.050 CPU sec, 2.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293031, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (232.46, 111.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703438 sequences (100000224 bp) in 1.400 CPU sec, 1.407 real sec...
[M::mem_process_seqs] Processed 701432 reads in 142.139 CPU sec, 4.521 real sec
sorting block 68, #reads = 1089798 mem = 536881191 peak = 1087352832
[M::mem_process_seqs] Processed 705248 reads in 84.971 CPU sec, 2.690 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292928, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (237.12, 113.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 775)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 69, #reads = 1090162 mem = 536878516 peak = 1087352832
[M::process] read 702720 sequences (100000074 bp) in 2.214 CPU sec, 2.334 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292847, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.46, 114.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703446 reads in 130.745 CPU sec, 4.160 real sec
[M::mem_process_seqs] Processed 703438 reads in 83.469 CPU sec, 2.630 real sec
[M::process] read 701908 sequences (100000161 bp) in 2.111 CPU sec, 2.165 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292860, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 210, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1372)
[M::mem_pestat] mean and std.dev: (281.56, 235.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1784)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (239.36, 115.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 70, #reads = 1095909 mem = 536879554 peak = 1087352832
[M::process] read 702060 sequences (100000177 bp) in 2.120 CPU sec, 2.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292119, 5, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.90, 116.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (312, 338, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (222, 447)
[M::mem_pestat] mean and std.dev: (334.38, 31.84)
[M::mem_pestat] low and high boundaries for proper pairs: (177, 492)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702720 reads in 130.335 CPU sec, 4.142 real sec
[M::mem_process_seqs] Processed 701908 reads in 82.305 CPU sec, 2.593 real sec
[M::process] read 701512 sequences (100000082 bp) in 1.603 CPU sec, 1.630 real sec...
sorting block 71, #reads = 1094764 mem = 536877108 peak = 1087352832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292502, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.65, 117.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 339, 813)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2031)
[M::mem_pestat] mean and std.dev: (517.90, 389.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2640)
[M::mem_pestat] skip orientation RR
[M::process] read 701032 sequences (100000261 bp) in 2.107 CPU sec, 2.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292608, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (243.63, 118.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 427, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (246.88, 140.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702060 reads in 135.705 CPU sec, 4.316 real sec
[M::process] read 700458 sequences (100000192 bp) in 1.380 CPU sec, 1.377 real sec...
[M::mem_process_seqs] Processed 701512 reads in 86.951 CPU sec, 2.744 real sec
sorting block 72, #reads = 1095870 mem = 536879422 peak = 1087352832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291465, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.03, 119.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698762 sequences (100000047 bp) in 2.610 CPU sec, 2.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292214, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.12, 120.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701032 reads in 129.950 CPU sec, 4.120 real sec
sorting block 73, #reads = 1093340 mem = 536879641 peak = 1087352832
[M::mem_process_seqs] Processed 700458 reads in 83.284 CPU sec, 2.624 real sec
[M::process] read 698814 sequences (100000179 bp) in 1.598 CPU sec, 1.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291122, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 196, 877)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2339)
[M::mem_pestat] mean and std.dev: (239.09, 208.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3070)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.42, 121.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 74, #reads = 1093574 mem = 536879855 peak = 1087352832
[M::process] read 698560 sequences (100000247 bp) in 2.227 CPU sec, 2.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291436, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.93, 120.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698762 reads in 133.426 CPU sec, 4.239 real sec
[M::process] read 699006 sequences (100000148 bp) in 1.417 CPU sec, 1.429 real sec...
[M::mem_process_seqs] Processed 698814 reads in 84.975 CPU sec, 2.681 real sec
sorting block 75, #reads = 1092516 mem = 536877633 peak = 1087352832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290894, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.41, 120.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698492 sequences (100000143 bp) in 2.231 CPU sec, 2.353 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291095, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 1355, 2067)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5979)
[M::mem_pestat] mean and std.dev: (1077.40, 924.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7935)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (247.97, 119.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698560 reads in 132.589 CPU sec, 4.215 real sec
[M::process] read 698424 sequences (100000142 bp) in 1.519 CPU sec, 1.535 real sec...
[M::mem_process_seqs] Processed 699006 reads in 85.045 CPU sec, 2.680 real sec
sorting block 76, #reads = 1092280 mem = 536882469 peak = 1087352832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290740, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 184, 246)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500)
[M::mem_pestat] mean and std.dev: (150.00, 54.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 627)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.83, 119.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698564 sequences (100000164 bp) in 2.067 CPU sec, 2.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290779, 10, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.55, 120.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (39, 405, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149)
[M::mem_pestat] mean and std.dev: (217.44, 173.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1519)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 701326 sequences (100000125 bp) in 1.500 CPU sec, 1.493 real sec...
[M::mem_process_seqs] Processed 698492 reads in 137.784 CPU sec, 4.379 real sec
[M::mem_process_seqs] Processed 698424 reads in 85.125 CPU sec, 2.690 real sec
sorting block 77, #reads = 1089010 mem = 536877500 peak = 1087352832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290695, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.09, 119.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 78, #reads = 1090677 mem = 536882392 peak = 1087352832
[M::process] read 708334 sequences (100000218 bp) in 2.533 CPU sec, 2.656 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291037, 8, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 171, 1929)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5469)
[M::mem_pestat] mean and std.dev: (798.15, 1086.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7239)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (241.13, 116.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698564 reads in 145.596 CPU sec, 4.634 real sec
[M::mem_process_seqs] Processed 701326 reads in 97.768 CPU sec, 3.079 real sec
[M::process] read 706532 sequences (100000274 bp) in 1.899 CPU sec, 1.935 real sec...
sorting block 79, #reads = 1087385 mem = 536894064 peak = 1087352832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 294079, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (224.25, 105.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705510 sequences (100000098 bp) in 2.671 CPU sec, 2.800 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293788, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (229.90, 109.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 708334 reads in 141.539 CPU sec, 4.504 real sec
[M::mem_process_seqs] Processed 706532 reads in 89.936 CPU sec, 2.835 real sec
[M::process] read 706480 sequences (100000045 bp) in 2.164 CPU sec, 2.226 real sec...
sorting block 80, #reads = 1090090 mem = 536875464 peak = 1087352832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293624, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (232.74, 111.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704718 sequences (100000188 bp) in 2.393 CPU sec, 2.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293726, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (228.87, 108.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 705510 reads in 142.152 CPU sec, 4.535 real sec
[M::mem_process_seqs] Processed 706480 reads in 92.441 CPU sec, 2.916 real sec
sorting block 81, #reads = 1093666 mem = 536879939 peak = 1087352832
[M::process] read 704488 sequences (100000038 bp) in 2.347 CPU sec, 2.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293254, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 209, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606)
[M::mem_pestat] mean and std.dev: (233.92, 112.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 758)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703282 sequences (100000225 bp) in 2.495 CPU sec, 2.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 293188, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (234.00, 112.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704718 reads in 140.636 CPU sec, 4.486 real sec
sorting block 82, #reads = 1092103 mem = 536877966 peak = 1087352832
[M::mem_process_seqs] Processed 704488 reads in 89.720 CPU sec, 2.834 real sec
[M::process] read 701574 sequences (100000105 bp) in 2.385 CPU sec, 2.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292065, 10, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.80, 115.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (815, 878, 1886)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4028)
[M::mem_pestat] mean and std.dev: (1043.62, 504.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5099)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 83, #reads = 1093669 mem = 536880923 peak = 1087352832
[M::process] read 700094 sequences (100000117 bp) in 2.404 CPU sec, 2.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292195, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (244.09, 119.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703282 reads in 139.596 CPU sec, 4.458 real sec
[M::mem_process_seqs] Processed 701574 reads in 87.944 CPU sec, 2.781 real sec
[M::process] read 699140 sequences (100000118 bp) in 2.460 CPU sec, 2.597 real sec...
sorting block 84, #reads = 1091934 mem = 536882372 peak = 1087352832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291641, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (245.85, 118.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699268 sequences (100000021 bp) in 2.428 CPU sec, 2.578 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291466, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.88, 119.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700094 reads in 145.843 CPU sec, 4.662 real sec
[M::mem_process_seqs] Processed 699140 reads in 95.710 CPU sec, 3.028 real sec
sorting block 85, #reads = 1091345 mem = 536876509 peak = 1087352832
[M::process] read 698770 sequences (100000276 bp) in 2.626 CPU sec, 2.858 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291514, 3, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 406, 675)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1563)
[M::mem_pestat] mean and std.dev: (380.89, 180.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2007)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.36, 118.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290594, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (406, 665, 2945)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8023)
[M::mem_pestat] mean and std.dev: (1383.45, 1221.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10562)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.15, 119.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 210, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (31, 441)
[M::mem_pestat] mean and std.dev: (208.00, 33.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 523)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698236 sequences (100000279 bp) in 2.422 CPU sec, 2.550 real sec...
[M::mem_process_seqs] Processed 699268 reads in 149.219 CPU sec, 4.785 real sec
[M::mem_process_seqs] Processed 698770 reads in 94.683 CPU sec, 3.002 real sec
sorting block 86, #reads = 1091156 mem = 536878608 peak = 1087352832
[M::process] read 697852 sequences (100000017 bp) in 2.431 CPU sec, 2.586 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290671, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.10, 120.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 87, #reads = 1091268 mem = 536885026 peak = 1087352832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290674, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.65, 120.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698236 reads in 143.249 CPU sec, 4.604 real sec
[M::process] read 697396 sequences (100000181 bp) in 2.649 CPU sec, 2.853 real sec...
[M::mem_process_seqs] Processed 697852 reads in 91.003 CPU sec, 2.898 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290915, 7, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 241, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058)
[M::mem_pestat] mean and std.dev: (299.20, 209.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1345)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.83, 121.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697602 sequences (100000070 bp) in 2.532 CPU sec, 2.703 real sec...
[M::mem_process_seqs] Processed 697396 reads in 76.429 CPU sec, 2.543 real sec
[M::process] read 47528 sequences (6789720 bp) in 0.571 CPU sec, 0.581 real sec...
sorting block 88, #reads = 1092126 mem = 536879401 peak = 1087352832
sorting block 89, #reads = 1091171 mem = 536881430 peak = 1110298624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290463, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (251.27, 121.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 19745, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 218, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (242.81, 114.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697602 reads in 81.792 CPU sec, 2.685 real sec
[M::mem_process_seqs] Processed 47528 reads in 18.705 CPU sec, 0.614 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 98695390
overall: 69993078784 mem 11339.172 user 338.853 sys 807.265 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L002\tPU:L002\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 807.300 sec; CPU: 11678.028 sec
sorting block 90, #reads = 811534 mem = 399808044 peak = 1110298624
uniq header count 1
merging 91 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5213995068
output reads: 99199633
bam_mem_sort: 91 calls 36.741 user 0.341 sys 37.216 real
bam_write: 91 calls 22.887 user 0.717 sys 23.721 real
execute: 1 calls 21.336 user 0.628 sys 835.081 real
merge_files: 1 calls 21.310 user 0.611 sys 27.161 real
parse_chunk: 1452 calls 53.565 user 13.293 sys 67.995 real
read_chunk: 3922 calls 36.511 user 34.576 sys 339.117 real
sort_block: 1 calls 28.167 user 33.976 sys 325.925 real
write_chunk: 2468 calls 20.893 user 0.423 sys 26.331 real
overall: 3071647744 mem 1079.944 user 59.446 sys 835.100 real
ls: cannot access '*.cram': No such file or directory