.command.err - Workdir - DAQ:SENTIEON_BWAMEM (FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1;L004;0001)

File Info

Filename: .command.err
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/17/0e10425e254d186b2f563e77d61299/.command.err
Size: 201.1 KB
Task: DAQ:SENTIEON_BWAMEM (FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1;L004;0001)
Attempt
Content

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1914.431 CPU sec, 72.549 real sec
[M::process] read 677638 sequences (100000161 bp) in 0.945 CPU sec, 0.948 real sec...
[M::process] read 677266 sequences (100000036 bp) in 1.200 CPU sec, 1.227 real sec...
[M::process] read 677176 sequences (100000032 bp) in 1.151 CPU sec, 1.162 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281684, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 358, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (370.21, 168.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 337, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (305.90, 125.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1322)
[M::mem_pestat] skip orientation RR
[M::process] read 676962 sequences (100000131 bp) in 1.118 CPU sec, 1.118 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281370, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 362, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (373.62, 169.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 222, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (227.50, 83.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281566, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 362, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (372.86, 167.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677638 reads in 233.573 CPU sec, 7.536 real sec
[M::mem_process_seqs] Processed 677266 reads in 157.320 CPU sec, 5.135 real sec
[M::mem_process_seqs] Processed 677176 reads in 169.447 CPU sec, 5.542 real sec
sorting block 0, #reads = 1067016 mem = 536881115 peak = 598839296
[M::process] read 676792 sequences (100000022 bp) in 2.161 CPU sec, 2.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281123, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 363, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (374.19, 168.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676028 sequences (100000283 bp) in 1.180 CPU sec, 1.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281619, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 367, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (377.98, 169.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675818 sequences (100000088 bp) in 1.160 CPU sec, 1.157 real sec...
[M::mem_process_seqs] Processed 676962 reads in 151.634 CPU sec, 4.806 real sec
sorting block 1, #reads = 1065783 mem = 536881620 peak = 886218752
[M::mem_process_seqs] Processed 676792 reads in 95.004 CPU sec, 3.003 real sec
sorting block 2, #reads = 1065443 mem = 536879822 peak = 886218752
[M::process] read 675512 sequences (100000219 bp) in 1.638 CPU sec, 1.737 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281190, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 371, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (381.86, 170.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675232 sequences (100000116 bp) in 1.276 CPU sec, 1.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 280916, 0, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 283, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539)
[M::mem_pestat] mean and std.dev: (234.00, 76.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 667)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 375, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (385.06, 171.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 301, 2866)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8198)
[M::mem_pestat] mean and std.dev: (963.53, 1157.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10864)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280776, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 380, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (388.68, 172.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 382, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (359.10, 170.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 676028 reads in 219.942 CPU sec, 7.066 real sec
[M::mem_process_seqs] Processed 675818 reads in 161.043 CPU sec, 5.203 real sec
[M::mem_process_seqs] Processed 675512 reads in 174.630 CPU sec, 5.661 real sec
sorting block 3, #reads = 1064925 mem = 536878980 peak = 886218752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280608, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 381, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (389.44, 172.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675166 sequences (100000102 bp) in 2.434 CPU sec, 2.587 real sec...
[M::mem_process_seqs] Processed 675232 reads in 85.687 CPU sec, 2.811 real sec
sorting block 4, #reads = 1064716 mem = 536879769 peak = 886218752
[M::process] read 675076 sequences (100000180 bp) in 2.198 CPU sec, 2.371 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280473, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 384, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (392.17, 173.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674976 sequences (100000257 bp) in 1.710 CPU sec, 1.709 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280778, 1, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (357, 828, 828)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1770)
[M::mem_pestat] mean and std.dev: (689.70, 220.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2241)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 384, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (393.24, 174.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675166 reads in 156.945 CPU sec, 4.953 real sec
[M::mem_process_seqs] Processed 675076 reads in 96.856 CPU sec, 3.038 real sec
sorting block 5, #reads = 1064915 mem = 536879191 peak = 886218752
[M::process] read 675150 sequences (100000059 bp) in 1.887 CPU sec, 1.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 280724, 0, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (237, 308, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (65, 495)
[M::mem_pestat] mean and std.dev: (263.53, 80.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 587)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (391.54, 173.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 6, #reads = 1065105 mem = 536880810 peak = 886218752
[M::process] read 675004 sequences (100000241 bp) in 2.266 CPU sec, 2.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 281064, 0, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (259, 259, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (19, 619)
[M::mem_pestat] mean and std.dev: (307.27, 61.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (389.70, 172.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674976 reads in 156.234 CPU sec, 4.968 real sec
[M::process] read 675224 sequences (100000003 bp) in 1.700 CPU sec, 1.694 real sec...
[M::mem_process_seqs] Processed 675150 reads in 97.641 CPU sec, 3.077 real sec
sorting block 7, #reads = 1065568 mem = 536878054 peak = 886218752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280287, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 380, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (389.68, 172.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675154 sequences (100000053 bp) in 2.433 CPU sec, 2.548 real sec...
[M::process] read 675104 sequences (100000092 bp) in 1.764 CPU sec, 1.768 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281005, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (388.23, 172.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675004 reads in 158.056 CPU sec, 5.016 real sec
[M::mem_process_seqs] Processed 675224 reads in 95.551 CPU sec, 3.029 real sec
sorting block 8, #reads = 1064579 mem = 536879445 peak = 886218752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280802, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (386.01, 171.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675368 sequences (100000248 bp) in 2.080 CPU sec, 2.229 real sec...
[M::process] read 675122 sequences (100000115 bp) in 1.310 CPU sec, 1.308 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 280037, 0, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (309, 309, 1042)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2508)
[M::mem_pestat] mean and std.dev: (734.91, 753.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3750)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (386.97, 171.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675154 reads in 154.543 CPU sec, 4.921 real sec
[M::mem_process_seqs] Processed 675104 reads in 95.431 CPU sec, 3.044 real sec
sorting block 9, #reads = 1065167 mem = 536879239 peak = 886218752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 280596, 0, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (203, 308, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (295.80, 101.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 377, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (387.37, 172.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674924 sequences (100000049 bp) in 2.463 CPU sec, 2.572 real sec...
[M::process] read 674950 sequences (100000073 bp) in 1.760 CPU sec, 1.765 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280042, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (388.49, 172.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675368 reads in 160.940 CPU sec, 5.101 real sec
sorting block 10, #reads = 1064168 mem = 536879779 peak = 886218752
[M::mem_process_seqs] Processed 675122 reads in 98.600 CPU sec, 3.119 real sec
sorting block 11, #reads = 1064661 mem = 536883515 peak = 886218752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280209, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (389.52, 172.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674744 sequences (100000100 bp) in 2.536 CPU sec, 2.677 real sec...
[M::process] read 674546 sequences (100000202 bp) in 1.770 CPU sec, 1.776 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279897, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 380, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (390.69, 173.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674924 reads in 164.034 CPU sec, 5.208 real sec
[M::mem_process_seqs] Processed 674950 reads in 99.256 CPU sec, 3.138 real sec
sorting block 12, #reads = 1065212 mem = 536879569 peak = 886218752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280021, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 381, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (390.04, 172.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674302 sequences (100000128 bp) in 2.583 CPU sec, 2.716 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280524, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (391.74, 172.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674264 sequences (100000244 bp) in 1.760 CPU sec, 1.760 real sec...
[M::mem_process_seqs] Processed 674744 reads in 157.837 CPU sec, 5.002 real sec
[M::mem_process_seqs] Processed 674546 reads in 97.432 CPU sec, 3.073 real sec
sorting block 13, #reads = 1064067 mem = 536878120 peak = 886218752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280223, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 383, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (392.08, 171.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674328 sequences (100000272 bp) in 2.614 CPU sec, 2.749 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280261, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (391.73, 171.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673938 sequences (100000149 bp) in 1.740 CPU sec, 1.741 real sec...
[M::mem_process_seqs] Processed 674302 reads in 151.214 CPU sec, 4.782 real sec
[M::mem_process_seqs] Processed 674264 reads in 92.055 CPU sec, 2.901 real sec
sorting block 14, #reads = 1065090 mem = 536879588 peak = 886218752
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 280948, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (291, 291, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (199, 429)
[M::mem_pestat] mean and std.dev: (334.73, 39.12)
[M::mem_pestat] low and high boundaries for proper pairs: (153, 491)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 384, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (392.64, 171.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674006 sequences (100000065 bp) in 2.285 CPU sec, 2.421 real sec...
[M::process] read 673826 sequences (100000269 bp) in 1.390 CPU sec, 1.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279988, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 388, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (396.75, 173.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674328 reads in 149.073 CPU sec, 4.740 real sec
sorting block 15, #reads = 1064776 mem = 536879603 peak = 886218752
[M::mem_process_seqs] Processed 673938 reads in 91.799 CPU sec, 2.906 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280548, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 390, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (399.28, 174.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1064256 mem = 536877657 peak = 946520064
[M::process] read 673606 sequences (100000113 bp) in 2.640 CPU sec, 2.772 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280239, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 391, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (400.04, 175.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674006 reads in 146.541 CPU sec, 4.646 real sec
[M::process] read 674850 sequences (100000218 bp) in 1.807 CPU sec, 1.831 real sec...
[M::mem_process_seqs] Processed 673826 reads in 93.516 CPU sec, 2.939 real sec
sorting block 17, #reads = 1065875 mem = 536879231 peak = 946520064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279988, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 395, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (403.69, 176.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677454 sequences (100000126 bp) in 2.591 CPU sec, 2.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280870, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 384, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (393.29, 173.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677024 sequences (100000178 bp) in 1.426 CPU sec, 1.439 real sec...
[M::mem_process_seqs] Processed 673606 reads in 148.424 CPU sec, 4.715 real sec
[M::mem_process_seqs] Processed 674850 reads in 94.067 CPU sec, 2.975 real sec
sorting block 18, #reads = 1065002 mem = 536878700 peak = 946520064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280340, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 364, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (375.17, 170.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676816 sequences (100000081 bp) in 2.684 CPU sec, 2.820 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280588, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 368, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (378.85, 171.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 364, 953)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2359)
[M::mem_pestat] mean and std.dev: (532.70, 381.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3062)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 677454 reads in 153.169 CPU sec, 4.852 real sec
[M::process] read 676652 sequences (100000169 bp) in 1.814 CPU sec, 1.815 real sec...
[M::mem_process_seqs] Processed 677024 reads in 95.429 CPU sec, 3.004 real sec
sorting block 19, #reads = 1064965 mem = 536878967 peak = 946520064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280684, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 369, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (299.90, 89.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 368, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (378.56, 171.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (299, 352, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (169, 494)
[M::mem_pestat] mean and std.dev: (314.30, 65.99)
[M::mem_pestat] low and high boundaries for proper pairs: (50, 578)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 20, #reads = 1067158 mem = 536879268 peak = 946520064
[M::process] read 676492 sequences (100000185 bp) in 2.652 CPU sec, 2.795 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280433, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 371, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (380.27, 171.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676816 reads in 151.056 CPU sec, 4.783 real sec
[M::process] read 676250 sequences (100000252 bp) in 1.842 CPU sec, 1.845 real sec...
[M::mem_process_seqs] Processed 676652 reads in 92.543 CPU sec, 2.910 real sec
sorting block 21, #reads = 1067388 mem = 536878642 peak = 946520064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280680, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 371, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (380.39, 171.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676028 sequences (100000096 bp) in 2.658 CPU sec, 2.794 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281106, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (384.52, 171.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676492 reads in 149.606 CPU sec, 4.740 real sec
[M::process] read 675344 sequences (100000078 bp) in 1.828 CPU sec, 1.838 real sec...
[M::mem_process_seqs] Processed 676250 reads in 93.194 CPU sec, 2.931 real sec
sorting block 22, #reads = 1066393 mem = 536877004 peak = 946520064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281048, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (385.24, 171.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675176 sequences (100000119 bp) in 2.637 CPU sec, 2.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280436, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (389.68, 173.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676028 reads in 150.795 CPU sec, 4.784 real sec
[M::process] read 675060 sequences (100000072 bp) in 1.569 CPU sec, 1.574 real sec...
[M::mem_process_seqs] Processed 675344 reads in 93.320 CPU sec, 2.939 real sec
sorting block 23, #reads = 1066633 mem = 536878766 peak = 946520064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280297, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 383, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (391.23, 173.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675132 sequences (100000172 bp) in 2.379 CPU sec, 2.502 real sec...
[M::process] read 675122 sequences (100000030 bp) in 1.350 CPU sec, 1.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280429, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 388, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (395.54, 174.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 527, 1019)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2811)
[M::mem_pestat] mean and std.dev: (628.83, 540.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3707)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675176 reads in 148.006 CPU sec, 4.703 real sec
sorting block 24, #reads = 1065785 mem = 536878717 peak = 946520064
[M::process] read 675074 sequences (100000251 bp) in 1.601 CPU sec, 1.609 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280607, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 385, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (393.05, 174.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675132 reads in 88.661 CPU sec, 2.996 real sec
sorting block 25, #reads = 1065770 mem = 536885180 peak = 946520064
[M::mem_process_seqs] Processed 675060 reads in 203.602 CPU sec, 6.649 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280384, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (394.03, 174.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675212 sequences (100000275 bp) in 2.523 CPU sec, 2.650 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280629, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 387, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (395.37, 176.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675122 reads in 164.640 CPU sec, 5.224 real sec
[M::mem_process_seqs] Processed 675074 reads in 95.704 CPU sec, 3.009 real sec
sorting block 26, #reads = 1065975 mem = 536881842 peak = 946520064
[M::process] read 674838 sequences (100000143 bp) in 2.122 CPU sec, 2.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280477, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 384, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (393.24, 175.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 27, #reads = 1065860 mem = 536881349 peak = 946520064
[M::process] read 675244 sequences (100000209 bp) in 2.292 CPU sec, 2.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281005, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 384, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (393.42, 175.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 256, 745)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1757)
[M::mem_pestat] mean and std.dev: (428.83, 273.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2263)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675212 reads in 154.108 CPU sec, 4.898 real sec
[M::process] read 675318 sequences (100000273 bp) in 1.628 CPU sec, 1.644 real sec...
[M::mem_process_seqs] Processed 674838 reads in 95.360 CPU sec, 3.000 real sec
sorting block 28, #reads = 1065110 mem = 536877982 peak = 946520064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280795, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 382, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (391.12, 174.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (575, 575, 575)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (575, 575)
[M::mem_pestat] mean and std.dev: (575.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (575, 575)
[M::mem_pestat] skip orientation RR
[M::process] read 675534 sequences (100000296 bp) in 2.650 CPU sec, 2.782 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280694, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 375, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (385.71, 171.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675652 sequences (100000025 bp) in 1.570 CPU sec, 1.568 real sec...
[M::mem_process_seqs] Processed 675244 reads in 157.872 CPU sec, 5.007 real sec
[M::mem_process_seqs] Processed 675318 reads in 98.707 CPU sec, 3.117 real sec
sorting block 29, #reads = 1065446 mem = 536879324 peak = 946520064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 281011, 2, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (335, 374, 768)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1634)
[M::mem_pestat] mean and std.dev: (499.91, 294.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2067)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 374, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (383.68, 170.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675340 sequences (100000292 bp) in 2.316 CPU sec, 2.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280441, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 374, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (383.64, 170.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675072 sequences (100000141 bp) in 1.770 CPU sec, 1.775 real sec...
[M::mem_process_seqs] Processed 675534 reads in 153.664 CPU sec, 4.872 real sec
[M::mem_process_seqs] Processed 675652 reads in 94.915 CPU sec, 2.986 real sec
sorting block 30, #reads = 1065211 mem = 536880424 peak = 946520064
[M::process] read 675380 sequences (100000105 bp) in 2.432 CPU sec, 2.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280450, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 536, 1909)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5293)
[M::mem_pestat] mean and std.dev: (817.80, 727.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6985)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (386.63, 171.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675102 sequences (100000166 bp) in 1.790 CPU sec, 1.791 real sec...
[M::mem_process_seqs] Processed 675072 reads in 85.658 CPU sec, 2.692 real sec
sorting block 31, #reads = 1064470 mem = 536880356 peak = 946520064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280438, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (385.73, 171.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281016, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (386.61, 171.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674848 sequences (100000231 bp) in 1.478 CPU sec, 1.480 real sec...
[M::mem_process_seqs] Processed 675340 reads in 242.800 CPU sec, 7.711 real sec
[M::mem_process_seqs] Processed 675380 reads in 112.807 CPU sec, 3.595 real sec
sorting block 32, #reads = 1064346 mem = 536877307 peak = 946520064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281101, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 376, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (386.42, 171.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674688 sequences (100000014 bp) in 2.530 CPU sec, 2.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281282, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (391.97, 173.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675102 reads in 156.600 CPU sec, 4.986 real sec
[M::process] read 674562 sequences (100000055 bp) in 1.805 CPU sec, 1.819 real sec...
[M::mem_process_seqs] Processed 674848 reads in 99.890 CPU sec, 3.165 real sec
sorting block 33, #reads = 1065906 mem = 536879392 peak = 946520064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280610, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 385, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (394.30, 174.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 34, #reads = 1065164 mem = 536878594 peak = 946520064
[M::process] read 674250 sequences (100000183 bp) in 2.629 CPU sec, 2.833 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280803, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 384, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (393.62, 174.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674396 sequences (100000110 bp) in 1.710 CPU sec, 1.719 real sec...
[M::mem_process_seqs] Processed 674688 reads in 163.477 CPU sec, 5.241 real sec
[M::mem_process_seqs] Processed 674562 reads in 102.584 CPU sec, 3.273 real sec
sorting block 35, #reads = 1064623 mem = 536883728 peak = 946520064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280597, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 388, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (397.25, 175.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674328 sequences (100000027 bp) in 2.160 CPU sec, 2.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280688, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (396.31, 175.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674140 sequences (100000049 bp) in 1.572 CPU sec, 1.576 real sec...
[M::mem_process_seqs] Processed 674250 reads in 158.248 CPU sec, 5.067 real sec
[M::mem_process_seqs] Processed 674396 reads in 99.783 CPU sec, 3.167 real sec
sorting block 36, #reads = 1064673 mem = 536881749 peak = 946520064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 280731, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 259, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (228.10, 95.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 390, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (399.66, 177.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675522 sequences (100000129 bp) in 2.488 CPU sec, 2.681 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281635, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 393, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057)
[M::mem_pestat] mean and std.dev: (402.80, 180.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1322)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678310 sequences (100000207 bp) in 1.659 CPU sec, 1.665 real sec...
[M::mem_process_seqs] Processed 674328 reads in 157.128 CPU sec, 5.013 real sec
[M::mem_process_seqs] Processed 674140 reads in 101.078 CPU sec, 3.200 real sec
sorting block 37, #reads = 1065453 mem = 536880108 peak = 946520064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281202, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 384, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (394.18, 177.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677782 sequences (100000146 bp) in 1.977 CPU sec, 2.088 real sec...
[M::process] read 678024 sequences (100000284 bp) in 1.838 CPU sec, 1.842 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281387, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 356, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (367.40, 166.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675522 reads in 164.760 CPU sec, 5.263 real sec
[M::mem_process_seqs] Processed 678310 reads in 105.033 CPU sec, 3.334 real sec
sorting block 38, #reads = 1062691 mem = 536879125 peak = 946520064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281472, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 361, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (370.68, 166.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 39, #reads = 1066649 mem = 536878697 peak = 1015664640
[M::process] read 677512 sequences (100000023 bp) in 2.572 CPU sec, 2.768 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281450, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 360, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (368.48, 165.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677782 reads in 168.746 CPU sec, 5.420 real sec
[M::process] read 677070 sequences (100000273 bp) in 1.875 CPU sec, 1.895 real sec...
[M::mem_process_seqs] Processed 678024 reads in 107.759 CPU sec, 3.425 real sec
sorting block 40, #reads = 1066167 mem = 536881236 peak = 1015664640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281049, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 361, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (370.06, 165.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676696 sequences (100000250 bp) in 2.322 CPU sec, 2.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280945, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 364, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (372.14, 165.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676530 sequences (100000299 bp) in 1.805 CPU sec, 1.815 real sec...
[M::mem_process_seqs] Processed 677512 reads in 168.115 CPU sec, 5.388 real sec
[M::mem_process_seqs] Processed 677070 reads in 106.347 CPU sec, 3.389 real sec
sorting block 41, #reads = 1066359 mem = 536879134 peak = 1015664640
[M::process] read 675962 sequences (100000065 bp) in 2.645 CPU sec, 2.906 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280819, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 370, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (377.65, 166.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676064 sequences (100000012 bp) in 1.787 CPU sec, 1.793 real sec...
[M::mem_process_seqs] Processed 676530 reads in 96.738 CPU sec, 3.071 real sec
sorting block 42, #reads = 1065680 mem = 536877667 peak = 1015664640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280987, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 367, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (374.53, 164.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280157, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 372, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (379.73, 166.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676696 reads in 259.769 CPU sec, 8.352 real sec
[M::process] read 675406 sequences (100000040 bp) in 2.057 CPU sec, 2.159 real sec...
[M::mem_process_seqs] Processed 675962 reads in 123.207 CPU sec, 3.964 real sec
sorting block 43, #reads = 1065303 mem = 536881719 peak = 1015664640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280302, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 372, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (379.75, 166.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675492 sequences (100000233 bp) in 2.442 CPU sec, 2.651 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280317, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 377, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (384.66, 168.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675424 sequences (100000267 bp) in 1.728 CPU sec, 1.735 real sec...
[M::mem_process_seqs] Processed 676064 reads in 169.855 CPU sec, 5.433 real sec
sorting block 44, #reads = 1065746 mem = 536879634 peak = 1015664640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280101, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 376, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (382.93, 167.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675224 sequences (100000231 bp) in 2.143 CPU sec, 2.150 real sec...
[M::mem_process_seqs] Processed 675492 reads in 92.775 CPU sec, 2.943 real sec
[M::mem_process_seqs] Processed 675406 reads in 241.009 CPU sec, 7.671 real sec
sorting block 45, #reads = 1065045 mem = 536880277 peak = 1015664640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 280440, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (278, 278, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 512)
[M::mem_pestat] mean and std.dev: (293.86, 67.21)
[M::mem_pestat] low and high boundaries for proper pairs: (25, 590)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 378, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (385.47, 168.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675014 sequences (100000133 bp) in 2.381 CPU sec, 2.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280487, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 380, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (387.20, 169.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675424 reads in 182.812 CPU sec, 5.821 real sec
[M::mem_process_seqs] Processed 675224 reads in 106.796 CPU sec, 3.370 real sec
[M::process] read 675454 sequences (100000131 bp) in 1.959 CPU sec, 2.012 real sec...
sorting block 46, #reads = 1065440 mem = 536880830 peak = 1015664640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280636, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 379, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (386.77, 168.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 286, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (174, 409)
[M::mem_pestat] mean and std.dev: (288.67, 19.75)
[M::mem_pestat] low and high boundaries for proper pairs: (127, 456)
[M::mem_pestat] skip orientation RR
[M::process] read 675472 sequences (100000077 bp) in 2.419 CPU sec, 2.559 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280541, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 375, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (383.86, 169.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675264 sequences (100000168 bp) in 1.663 CPU sec, 1.671 real sec...
[M::mem_process_seqs] Processed 675014 reads in 168.555 CPU sec, 5.377 real sec
sorting block 47, #reads = 1065027 mem = 536880185 peak = 1015664640
[M::mem_process_seqs] Processed 675454 reads in 106.186 CPU sec, 3.368 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280658, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 373, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (382.97, 169.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 48, #reads = 1064622 mem = 536877793 peak = 1015664640
[M::process] read 675362 sequences (100000023 bp) in 2.404 CPU sec, 2.682 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280756, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 376, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (384.67, 169.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675460 sequences (100000262 bp) in 1.567 CPU sec, 1.578 real sec...
[M::mem_process_seqs] Processed 675472 reads in 172.065 CPU sec, 5.519 real sec
[M::mem_process_seqs] Processed 675264 reads in 106.433 CPU sec, 3.379 real sec
sorting block 49, #reads = 1065410 mem = 536877032 peak = 1015664640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280091, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 374, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (383.27, 169.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675316 sequences (100000242 bp) in 2.396 CPU sec, 2.495 real sec...
[M::process] read 675174 sequences (100000025 bp) in 1.786 CPU sec, 1.794 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279893, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 374, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (383.55, 169.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675362 reads in 168.610 CPU sec, 5.349 real sec
[M::mem_process_seqs] Processed 675460 reads in 105.102 CPU sec, 3.328 real sec
sorting block 50, #reads = 1064529 mem = 536879826 peak = 1015664640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280204, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 375, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (384.25, 169.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675132 sequences (100000140 bp) in 2.513 CPU sec, 2.760 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280036, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 379, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (387.74, 170.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675004 sequences (100000254 bp) in 1.850 CPU sec, 1.851 real sec...
[M::mem_process_seqs] Processed 675316 reads in 172.638 CPU sec, 5.523 real sec
[M::mem_process_seqs] Processed 675174 reads in 106.992 CPU sec, 3.394 real sec
sorting block 51, #reads = 1064201 mem = 536880690 peak = 1015664640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280271, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 373, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (383.27, 168.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674938 sequences (100000250 bp) in 2.629 CPU sec, 2.816 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280372, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 376, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (385.09, 168.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674780 sequences (100000126 bp) in 1.863 CPU sec, 1.883 real sec...
[M::mem_process_seqs] Processed 675132 reads in 169.868 CPU sec, 5.422 real sec
sorting block 52, #reads = 1063657 mem = 536879785 peak = 1015664640
[M::mem_process_seqs] Processed 675004 reads in 106.562 CPU sec, 3.384 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280478, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (389.14, 171.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1064197 mem = 536878671 peak = 1021136896
[M::process] read 674808 sequences (100000018 bp) in 2.545 CPU sec, 2.779 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 280317, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (259, 344, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (303.82, 80.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1075)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 384, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (392.73, 173.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674938 reads in 169.830 CPU sec, 5.448 real sec
[M::process] read 675038 sequences (100000163 bp) in 1.947 CPU sec, 1.982 real sec...
[M::mem_process_seqs] Processed 674780 reads in 107.685 CPU sec, 3.407 real sec
sorting block 54, #reads = 1063192 mem = 536880306 peak = 1021136896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280639, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 384, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (393.19, 174.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674740 sequences (100000145 bp) in 2.621 CPU sec, 2.908 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280999, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 382, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (392.39, 175.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674808 reads in 164.166 CPU sec, 5.292 real sec
[M::mem_process_seqs] Processed 675038 reads in 103.485 CPU sec, 3.270 real sec
[M::process] read 674664 sequences (100000064 bp) in 1.991 CPU sec, 2.025 real sec...
sorting block 55, #reads = 1061800 mem = 536879710 peak = 1021136896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281273, 7, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 266, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448)
[M::mem_pestat] mean and std.dev: (215.62, 51.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 539)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (395.19, 177.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1304)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675930 sequences (100000013 bp) in 2.538 CPU sec, 2.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281155, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 384, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (395.41, 177.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674740 reads in 160.748 CPU sec, 5.197 real sec
[M::mem_process_seqs] Processed 674664 reads in 103.865 CPU sec, 3.349 real sec
[M::process] read 677532 sequences (100000139 bp) in 2.069 CPU sec, 2.187 real sec...
sorting block 56, #reads = 1064432 mem = 536880160 peak = 1021136896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281371, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 374, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (384.13, 172.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677362 sequences (100000193 bp) in 2.418 CPU sec, 2.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282082, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 361, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (371.22, 166.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 232, 235)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (163, 283)
[M::mem_pestat] mean and std.dev: (223.86, 11.19)
[M::mem_pestat] low and high boundaries for proper pairs: (139, 307)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675930 reads in 171.827 CPU sec, 5.559 real sec
sorting block 57, #reads = 1063870 mem = 536878677 peak = 1036685312
[M::process] read 677356 sequences (100000062 bp) in 1.821 CPU sec, 1.839 real sec...
[M::mem_process_seqs] Processed 677532 reads in 108.747 CPU sec, 3.505 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280411, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 364, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (373.80, 168.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1064702 mem = 536878074 peak = 1036685312
[M::process] read 676898 sequences (100000101 bp) in 2.319 CPU sec, 2.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281807, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 364, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (373.65, 167.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677362 reads in 168.090 CPU sec, 5.532 real sec
[M::mem_process_seqs] Processed 677356 reads in 107.306 CPU sec, 3.502 real sec
[M::process] read 676606 sequences (100000201 bp) in 2.079 CPU sec, 2.226 real sec...
sorting block 59, #reads = 1067831 mem = 536879621 peak = 1036685312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280280, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 366, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (375.32, 167.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676664 sequences (100000278 bp) in 2.450 CPU sec, 2.687 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280195, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 369, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (377.43, 167.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676898 reads in 169.811 CPU sec, 5.545 real sec
[M::process] read 676292 sequences (100000072 bp) in 1.903 CPU sec, 1.984 real sec...
[M::mem_process_seqs] Processed 676606 reads in 108.821 CPU sec, 3.562 real sec
sorting block 60, #reads = 1066334 mem = 536878714 peak = 1036685312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281069, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 369, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (377.68, 167.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676052 sequences (100000087 bp) in 2.445 CPU sec, 2.695 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279826, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 372, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (379.82, 167.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676664 reads in 170.096 CPU sec, 5.579 real sec
[M::mem_process_seqs] Processed 676292 reads in 107.978 CPU sec, 3.500 real sec
[M::process] read 675904 sequences (100000008 bp) in 2.078 CPU sec, 2.171 real sec...
sorting block 61, #reads = 1066131 mem = 536879668 peak = 1036685312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279843, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 375, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (382.54, 168.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 141, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (178.67, 71.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1045)
[M::mem_pestat] skip orientation RR
sorting block 62, #reads = 1065878 mem = 536877373 peak = 1036685312
[M::process] read 675852 sequences (100000290 bp) in 2.577 CPU sec, 2.758 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280060, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 378, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (383.98, 168.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676052 reads in 163.734 CPU sec, 5.287 real sec
[M::mem_process_seqs] Processed 675904 reads in 100.696 CPU sec, 3.183 real sec
[M::process] read 675704 sequences (100000017 bp) in 1.753 CPU sec, 1.806 real sec...
sorting block 63, #reads = 1064746 mem = 536878608 peak = 1036685312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279775, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 375, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (382.61, 167.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675708 sequences (100000124 bp) in 2.260 CPU sec, 2.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280069, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 377, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (383.50, 167.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675476 sequences (100000123 bp) in 1.760 CPU sec, 1.755 real sec...
[M::mem_process_seqs] Processed 675852 reads in 159.624 CPU sec, 5.071 real sec
[M::mem_process_seqs] Processed 675704 reads in 99.260 CPU sec, 3.134 real sec
sorting block 64, #reads = 1064678 mem = 536878202 peak = 1036685312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 279925, 4, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (250, 311, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 433)
[M::mem_pestat] mean and std.dev: (268.44, 45.70)
[M::mem_pestat] low and high boundaries for proper pairs: (67, 494)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 378, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (384.89, 168.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675624 sequences (100000206 bp) in 2.651 CPU sec, 2.769 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280130, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 378, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (384.20, 167.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675900 sequences (100000221 bp) in 1.839 CPU sec, 1.848 real sec...
[M::mem_process_seqs] Processed 675708 reads in 160.038 CPU sec, 5.066 real sec
[M::mem_process_seqs] Processed 675476 reads in 99.303 CPU sec, 3.130 real sec
sorting block 65, #reads = 1065509 mem = 536879535 peak = 1036685312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279465, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (386.16, 170.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676778 sequences (100000202 bp) in 2.062 CPU sec, 2.186 real sec...
[M::process] read 676028 sequences (100000209 bp) in 1.490 CPU sec, 1.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280198, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 375, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (383.60, 170.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675624 reads in 164.784 CPU sec, 5.246 real sec
sorting block 66, #reads = 1063793 mem = 536879561 peak = 1036685312
[M::mem_process_seqs] Processed 675900 reads in 106.029 CPU sec, 3.368 real sec
sorting block 67, #reads = 1064723 mem = 536879420 peak = 1082097664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281315, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 362, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (374.11, 169.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676008 sequences (100000065 bp) in 2.254 CPU sec, 2.377 real sec...
[M::process] read 675974 sequences (100000120 bp) in 1.517 CPU sec, 1.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280416, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 369, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (378.53, 167.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676778 reads in 169.058 CPU sec, 5.385 real sec
[M::mem_process_seqs] Processed 676028 reads in 106.351 CPU sec, 3.378 real sec
sorting block 68, #reads = 1064077 mem = 536878712 peak = 1082097664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280430, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 367, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (377.03, 166.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675724 sequences (100000258 bp) in 2.395 CPU sec, 2.518 real sec...
[M::process] read 675970 sequences (100000157 bp) in 1.767 CPU sec, 1.775 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280300, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 368, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (376.30, 165.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676008 reads in 173.764 CPU sec, 5.538 real sec
[M::mem_process_seqs] Processed 675974 reads in 111.584 CPU sec, 3.551 real sec
sorting block 69, #reads = 1064249 mem = 536878628 peak = 1082097664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280273, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 366, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (375.65, 164.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675738 sequences (100000231 bp) in 2.611 CPU sec, 2.747 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280435, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 367, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (377.17, 165.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675586 sequences (100000087 bp) in 1.940 CPU sec, 1.945 real sec...
[M::mem_process_seqs] Processed 675724 reads in 175.824 CPU sec, 5.607 real sec
[M::mem_process_seqs] Processed 675970 reads in 111.573 CPU sec, 3.535 real sec
sorting block 70, #reads = 1063655 mem = 536880064 peak = 1082097664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280619, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 365, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (374.74, 164.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 384, 890)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2184)
[M::mem_pestat] mean and std.dev: (414.10, 270.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2831)
[M::mem_pestat] skip orientation RR
sorting block 71, #reads = 1063870 mem = 536880591 peak = 1082097664
[M::process] read 675530 sequences (100000071 bp) in 2.514 CPU sec, 2.646 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280760, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 364, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (374.33, 163.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675390 sequences (100000037 bp) in 1.866 CPU sec, 1.869 real sec...
[M::mem_process_seqs] Processed 675738 reads in 177.319 CPU sec, 5.662 real sec
[M::mem_process_seqs] Processed 675586 reads in 110.826 CPU sec, 3.506 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280406, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 365, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (374.38, 163.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 72, #reads = 1064394 mem = 536880057 peak = 1082097664
[M::process] read 675134 sequences (100000255 bp) in 2.863 CPU sec, 3.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281145, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 373, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (381.11, 166.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675530 reads in 173.319 CPU sec, 5.521 real sec
[M::process] read 675072 sequences (100000197 bp) in 1.920 CPU sec, 1.928 real sec...
[M::mem_process_seqs] Processed 675390 reads in 109.756 CPU sec, 3.469 real sec
sorting block 73, #reads = 1063883 mem = 536884796 peak = 1082097664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281057, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 377, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (385.20, 168.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674740 sequences (100000044 bp) in 2.612 CPU sec, 2.769 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 280524, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (234.50, 72.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 379, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (387.77, 171.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675134 reads in 171.997 CPU sec, 5.490 real sec
[M::process] read 674502 sequences (100000042 bp) in 1.951 CPU sec, 1.965 real sec...
[M::mem_process_seqs] Processed 675072 reads in 109.008 CPU sec, 3.443 real sec
sorting block 74, #reads = 1064466 mem = 536879942 peak = 1082097664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280931, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (389.68, 172.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677238 sequences (100000031 bp) in 2.865 CPU sec, 3.021 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280646, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 385, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (393.92, 173.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674740 reads in 168.751 CPU sec, 5.385 real sec
[M::process] read 680396 sequences (100000242 bp) in 1.961 CPU sec, 1.981 real sec...
sorting block 75, #reads = 1062853 mem = 536880149 peak = 1082097664
[M::mem_process_seqs] Processed 674502 reads in 107.655 CPU sec, 3.400 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281490, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 365, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (377.79, 173.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 76, #reads = 1064127 mem = 536876975 peak = 1082097664
[M::process] read 679206 sequences (100000200 bp) in 2.338 CPU sec, 2.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283664, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 338, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (356.86, 170.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677876 sequences (100000253 bp) in 1.865 CPU sec, 1.870 real sec...
[M::mem_process_seqs] Processed 677238 reads in 180.415 CPU sec, 5.763 real sec
[M::mem_process_seqs] Processed 680396 reads in 115.496 CPU sec, 3.662 real sec
sorting block 77, #reads = 1062991 mem = 536877500 peak = 1082097664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282940, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 349, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (364.40, 171.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677044 sequences (100000287 bp) in 2.927 CPU sec, 3.070 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282377, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 364, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (376.88, 175.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676970 sequences (100000043 bp) in 1.926 CPU sec, 1.928 real sec...
[M::mem_process_seqs] Processed 679206 reads in 180.236 CPU sec, 5.733 real sec
[M::mem_process_seqs] Processed 677876 reads in 111.379 CPU sec, 3.523 real sec
sorting block 78, #reads = 1064675 mem = 536878574 peak = 1082097664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281616, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 369, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (381.16, 175.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676572 sequences (100000221 bp) in 2.451 CPU sec, 2.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280892, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 371, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (382.10, 175.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676498 sequences (100000018 bp) in 1.934 CPU sec, 1.939 real sec...
[M::mem_process_seqs] Processed 677044 reads in 175.070 CPU sec, 5.586 real sec
[M::mem_process_seqs] Processed 676970 reads in 110.636 CPU sec, 3.497 real sec
sorting block 79, #reads = 1065182 mem = 536878376 peak = 1082097664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281342, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 375, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (385.01, 175.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676390 sequences (100000266 bp) in 2.394 CPU sec, 2.526 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281129, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 375, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (384.73, 174.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676126 sequences (100000233 bp) in 1.959 CPU sec, 1.961 real sec...
[M::mem_process_seqs] Processed 676572 reads in 176.932 CPU sec, 5.645 real sec
sorting block 80, #reads = 1065048 mem = 536881539 peak = 1082097664
[M::mem_process_seqs] Processed 676498 reads in 112.602 CPU sec, 3.570 real sec
sorting block 81, #reads = 1063724 mem = 536885796 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281311, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 377, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (385.71, 174.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676034 sequences (100000109 bp) in 2.448 CPU sec, 2.587 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281440, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 378, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (386.96, 174.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675870 sequences (100000110 bp) in 1.920 CPU sec, 1.915 real sec...
[M::mem_process_seqs] Processed 676390 reads in 178.232 CPU sec, 5.682 real sec
[M::mem_process_seqs] Processed 676126 reads in 112.503 CPU sec, 3.557 real sec
sorting block 82, #reads = 1062630 mem = 536879371 peak = 1107951616
[M::process] read 675822 sequences (100000024 bp) in 2.790 CPU sec, 2.938 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281111, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 379, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (386.92, 172.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675644 sequences (100000250 bp) in 1.843 CPU sec, 1.846 real sec...
[M::mem_process_seqs] Processed 675870 reads in 103.018 CPU sec, 3.254 real sec
sorting block 83, #reads = 1063458 mem = 536879393 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280714, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 379, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (387.73, 174.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1304)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281175, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 378, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (386.50, 172.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675688 sequences (100000277 bp) in 1.819 CPU sec, 1.828 real sec...
[M::mem_process_seqs] Processed 676034 reads in 275.049 CPU sec, 8.751 real sec
[M::mem_process_seqs] Processed 675822 reads in 128.867 CPU sec, 4.105 real sec
sorting block 84, #reads = 1062174 mem = 536878906 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281763, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 378, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (386.31, 171.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675642 sequences (100000004 bp) in 2.322 CPU sec, 2.467 real sec...
[M::process] read 675640 sequences (100000196 bp) in 1.900 CPU sec, 1.901 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281324, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 378, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (386.49, 171.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675644 reads in 174.767 CPU sec, 5.562 real sec
[M::mem_process_seqs] Processed 675688 reads in 110.218 CPU sec, 3.492 real sec
sorting block 85, #reads = 1062109 mem = 536879751 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281158, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 378, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (387.09, 172.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675728 sequences (100000152 bp) in 2.724 CPU sec, 2.873 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281660, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 378, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (386.24, 171.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 202, 560)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298)
[M::mem_pestat] mean and std.dev: (324.75, 171.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1667)
[M::mem_pestat] skip orientation RR
[M::process] read 675458 sequences (100000032 bp) in 1.987 CPU sec, 1.988 real sec...
[M::mem_process_seqs] Processed 675642 reads in 173.831 CPU sec, 5.511 real sec
[M::mem_process_seqs] Processed 675640 reads in 110.720 CPU sec, 3.497 real sec
sorting block 86, #reads = 1062558 mem = 536881681 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281364, 10, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 375, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (384.51, 170.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2359, 2367, 2367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2343, 2383)
[M::mem_pestat] mean and std.dev: (2363.44, 3.98)
[M::mem_pestat] low and high boundaries for proper pairs: (2335, 2391)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675446 sequences (100000169 bp) in 2.467 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281561, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 376, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (385.35, 170.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675388 sequences (100000041 bp) in 1.878 CPU sec, 1.884 real sec...
[M::mem_process_seqs] Processed 675728 reads in 178.679 CPU sec, 5.694 real sec
sorting block 87, #reads = 1063241 mem = 536877566 peak = 1107951616
[M::mem_process_seqs] Processed 675458 reads in 113.954 CPU sec, 3.603 real sec
sorting block 88, #reads = 1061003 mem = 536880714 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280639, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 377, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (386.29, 171.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675532 sequences (100000042 bp) in 2.769 CPU sec, 2.917 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281429, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 377, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (386.08, 171.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675424 sequences (100000105 bp) in 1.779 CPU sec, 1.785 real sec...
[M::mem_process_seqs] Processed 675446 reads in 178.367 CPU sec, 5.678 real sec
[M::mem_process_seqs] Processed 675388 reads in 111.467 CPU sec, 3.529 real sec
sorting block 89, #reads = 1061864 mem = 536877758 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280920, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (384.81, 169.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675080 sequences (100000180 bp) in 2.487 CPU sec, 2.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281389, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 375, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (384.75, 170.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (292, 335, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (206, 421)
[M::mem_pestat] mean and std.dev: (302.12, 29.04)
[M::mem_pestat] low and high boundaries for proper pairs: (163, 464)
[M::mem_pestat] skip orientation RR
[M::process] read 675344 sequences (100000215 bp) in 1.920 CPU sec, 1.920 real sec...
[M::mem_process_seqs] Processed 675532 reads in 178.925 CPU sec, 5.701 real sec
[M::mem_process_seqs] Processed 675424 reads in 112.699 CPU sec, 3.561 real sec
sorting block 90, #reads = 1061181 mem = 536878225 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280932, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (386.57, 170.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675274 sequences (100000166 bp) in 2.652 CPU sec, 2.784 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280331, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (386.70, 170.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675150 sequences (100000093 bp) in 1.967 CPU sec, 1.974 real sec...
[M::mem_process_seqs] Processed 675080 reads in 171.409 CPU sec, 5.438 real sec
sorting block 91, #reads = 1061522 mem = 536878042 peak = 1107951616
[M::mem_process_seqs] Processed 675344 reads in 108.165 CPU sec, 3.416 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281159, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 380, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (389.25, 173.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 92, #reads = 1061555 mem = 536878678 peak = 1107951616
[M::process] read 674752 sequences (100000227 bp) in 2.439 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281765, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (390.70, 174.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674958 sequences (100000059 bp) in 1.932 CPU sec, 1.940 real sec...
[M::mem_process_seqs] Processed 675274 reads in 172.027 CPU sec, 5.465 real sec
[M::mem_process_seqs] Processed 675150 reads in 108.280 CPU sec, 3.422 real sec
sorting block 93, #reads = 1061756 mem = 536880343 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281646, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 386, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (394.94, 175.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681802 sequences (100000190 bp) in 2.626 CPU sec, 2.731 real sec...
[M::process] read 681794 sequences (100000214 bp) in 1.618 CPU sec, 1.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281416, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 386, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (395.85, 177.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1312)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674752 reads in 171.000 CPU sec, 5.435 real sec
[M::mem_process_seqs] Processed 674958 reads in 109.119 CPU sec, 3.461 real sec
sorting block 94, #reads = 1061725 mem = 536878669 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283665, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 325, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (344.49, 164.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681790 sequences (100000187 bp) in 2.583 CPU sec, 2.703 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284178, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 326, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (344.05, 162.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680238 sequences (100000298 bp) in 1.990 CPU sec, 1.982 real sec...
[M::mem_process_seqs] Processed 681802 reads in 183.124 CPU sec, 5.820 real sec
[M::mem_process_seqs] Processed 681794 reads in 114.909 CPU sec, 3.631 real sec
sorting block 95, #reads = 1061152 mem = 536877659 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283780, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 326, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (342.47, 160.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679566 sequences (100000190 bp) in 2.815 CPU sec, 2.919 real sec...
[M::process] read 678920 sequences (100000015 bp) in 1.900 CPU sec, 1.895 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283348, 8, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (267, 284, 643)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395)
[M::mem_pestat] mean and std.dev: (422.36, 204.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1771)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 337, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (350.90, 162.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 319, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (302.58, 154.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681790 reads in 182.289 CPU sec, 5.785 real sec
sorting block 96, #reads = 1062557 mem = 536882292 peak = 1107951616
[M::mem_process_seqs] Processed 680238 reads in 112.291 CPU sec, 3.550 real sec
sorting block 97, #reads = 1060548 mem = 536883377 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283085, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 274, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472)
[M::mem_pestat] mean and std.dev: (230.56, 59.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 571)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 345, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (356.61, 163.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 678612 sequences (100000280 bp) in 2.269 CPU sec, 2.369 real sec...
[M::process] read 677830 sequences (100000115 bp) in 1.510 CPU sec, 1.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283388, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 351, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (361.49, 165.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679566 reads in 172.670 CPU sec, 5.496 real sec
[M::mem_process_seqs] Processed 678920 reads in 106.791 CPU sec, 3.389 real sec
sorting block 98, #reads = 1060818 mem = 536881781 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282953, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 354, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (364.74, 166.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677892 sequences (100000027 bp) in 2.676 CPU sec, 2.798 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282893, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677784 sequences (100000113 bp) in 1.700 CPU sec, 1.702 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (234, 361, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (370.14, 167.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678612 reads in 164.628 CPU sec, 5.216 real sec
[M::mem_process_seqs] Processed 677830 reads in 102.803 CPU sec, 3.246 real sec
sorting block 99, #reads = 1061853 mem = 536876810 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282878, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 358, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (367.91, 166.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677640 sequences (100000136 bp) in 2.392 CPU sec, 2.485 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282366, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 361, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (369.69, 165.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677214 sequences (100000069 bp) in 1.900 CPU sec, 1.898 real sec...
[M::mem_process_seqs] Processed 677892 reads in 165.167 CPU sec, 5.233 real sec
sorting block 100, #reads = 1061644 mem = 536878487 peak = 1107951616
[M::mem_process_seqs] Processed 677784 reads in 102.642 CPU sec, 3.240 real sec
sorting block 101, #reads = 1059709 mem = 536879765 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282580, 1, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 364, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (372.27, 167.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (403, 412, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (261, 616)
[M::mem_pestat] mean and std.dev: (429.92, 74.05)
[M::mem_pestat] low and high boundaries for proper pairs: (134, 726)
[M::mem_pestat] skip orientation RR
[M::process] read 676642 sequences (100000093 bp) in 2.511 CPU sec, 2.596 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282327, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 366, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (374.02, 167.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676670 sequences (100000269 bp) in 1.819 CPU sec, 1.830 real sec...
[M::mem_process_seqs] Processed 677640 reads in 160.486 CPU sec, 5.086 real sec
[M::mem_process_seqs] Processed 677214 reads in 102.069 CPU sec, 3.211 real sec
sorting block 102, #reads = 1060315 mem = 536879264 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282112, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 372, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (378.65, 168.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676478 sequences (100000197 bp) in 2.429 CPU sec, 2.514 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282298, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 372, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (379.83, 169.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676084 sequences (100000227 bp) in 1.810 CPU sec, 1.811 real sec...
[M::mem_process_seqs] Processed 676642 reads in 163.444 CPU sec, 5.183 real sec
[M::mem_process_seqs] Processed 676670 reads in 102.785 CPU sec, 3.241 real sec
sorting block 103, #reads = 1060699 mem = 536878811 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281752, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 371, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (380.05, 170.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675984 sequences (100000060 bp) in 2.187 CPU sec, 2.284 real sec...
[M::process] read 675742 sequences (100000201 bp) in 1.440 CPU sec, 1.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281600, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 373, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (381.61, 169.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676478 reads in 160.749 CPU sec, 5.109 real sec
sorting block 104, #reads = 1060597 mem = 536877819 peak = 1107951616
[M::mem_process_seqs] Processed 676084 reads in 98.577 CPU sec, 3.134 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281760, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 374, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (382.93, 170.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675638 sequences (100000121 bp) in 2.385 CPU sec, 2.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281949, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 376, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (384.71, 171.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675662 sequences (100000242 bp) in 1.830 CPU sec, 1.834 real sec...
[M::mem_process_seqs] Processed 675984 reads in 157.316 CPU sec, 4.984 real sec
sorting block 105, #reads = 1061623 mem = 536879841 peak = 1107951616
[M::mem_process_seqs] Processed 675742 reads in 97.766 CPU sec, 3.080 real sec
sorting block 106, #reads = 1060997 mem = 536879483 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280937, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 378, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (387.18, 172.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675750 sequences (100000198 bp) in 2.319 CPU sec, 2.413 real sec...
[M::process] read 675070 sequences (100000036 bp) in 1.430 CPU sec, 1.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281720, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 376, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (385.55, 171.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675638 reads in 154.530 CPU sec, 4.908 real sec
[M::mem_process_seqs] Processed 675662 reads in 95.771 CPU sec, 3.042 real sec
sorting block 107, #reads = 1062036 mem = 536878101 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281539, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 374, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (382.81, 170.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674850 sequences (100000171 bp) in 2.410 CPU sec, 2.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280737, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 381, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (390.78, 173.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674808 sequences (100000283 bp) in 1.750 CPU sec, 1.750 real sec...
[M::mem_process_seqs] Processed 675750 reads in 153.786 CPU sec, 4.875 real sec
[M::mem_process_seqs] Processed 675070 reads in 95.321 CPU sec, 3.008 real sec
sorting block 108, #reads = 1061538 mem = 536880120 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281012, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (391.35, 173.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674812 sequences (100000062 bp) in 2.681 CPU sec, 2.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281147, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 384, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (393.33, 174.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674850 reads in 149.578 CPU sec, 4.725 real sec
[M::process] read 675312 sequences (100000223 bp) in 1.954 CPU sec, 1.956 real sec...
sorting block 109, #reads = 1060673 mem = 536878506 peak = 1107951616
[M::mem_process_seqs] Processed 674808 reads in 92.703 CPU sec, 2.909 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280820, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 384, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (394.97, 176.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1311)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 110, #reads = 1062855 mem = 536878924 peak = 1107951616
[M::process] read 677276 sequences (100000047 bp) in 2.366 CPU sec, 2.456 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 281095, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (235, 329, 607)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351)
[M::mem_pestat] mean and std.dev: (366.75, 187.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1723)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 382, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050)
[M::mem_pestat] mean and std.dev: (393.64, 178.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1316)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674812 reads in 153.305 CPU sec, 4.860 real sec
[M::process] read 680628 sequences (100000023 bp) in 1.916 CPU sec, 1.913 real sec...
[M::mem_process_seqs] Processed 675312 reads in 96.527 CPU sec, 3.034 real sec
sorting block 111, #reads = 1061359 mem = 536882266 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281839, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 365, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (378.95, 175.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679644 sequences (100000058 bp) in 2.454 CPU sec, 2.556 real sec...
[M::process] read 679212 sequences (100000060 bp) in 1.410 CPU sec, 1.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282589, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 338, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (352.49, 164.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677276 reads in 164.796 CPU sec, 5.240 real sec
[M::mem_process_seqs] Processed 680628 reads in 99.196 CPU sec, 3.139 real sec
sorting block 112, #reads = 1056991 mem = 536879907 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281798, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 345, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (357.67, 165.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678310 sequences (100000101 bp) in 2.509 CPU sec, 2.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281805, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 349, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (361.54, 166.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679644 reads in 158.427 CPU sec, 5.018 real sec
[M::process] read 677986 sequences (100000015 bp) in 2.131 CPU sec, 2.139 real sec...
[M::mem_process_seqs] Processed 679212 reads in 96.470 CPU sec, 3.026 real sec
sorting block 113, #reads = 1065545 mem = 536878457 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281850, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 354, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (365.33, 166.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677638 sequences (100000011 bp) in 2.835 CPU sec, 2.950 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281807, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 358, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (368.30, 166.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678310 reads in 154.097 CPU sec, 4.888 real sec
sorting block 114, #reads = 1065905 mem = 536879290 peak = 1107951616
[M::mem_process_seqs] Processed 677986 reads in 95.548 CPU sec, 3.000 real sec
[M::process] read 677522 sequences (100000258 bp) in 2.251 CPU sec, 2.279 real sec...
sorting block 115, #reads = 1066177 mem = 536879577 peak = 1107951616
[M::process] read 676960 sequences (100000080 bp) in 2.168 CPU sec, 2.297 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281400, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 362, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (370.58, 165.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677522 reads in 84.784 CPU sec, 2.656 real sec
[M::process] read 676736 sequences (100000053 bp) in 1.865 CPU sec, 1.865 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281502, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 360, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (370.12, 166.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280941, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 366, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (374.43, 166.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677638 reads in 240.023 CPU sec, 7.610 real sec
[M::process] read 677190 sequences (100000263 bp) in 2.235 CPU sec, 2.256 real sec...
[M::mem_process_seqs] Processed 676960 reads in 109.952 CPU sec, 3.469 real sec
sorting block 116, #reads = 1065047 mem = 536877917 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280940, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 367, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (375.82, 167.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 117, #reads = 1064273 mem = 536880186 peak = 1107951616
[M::process] read 677098 sequences (100000281 bp) in 2.376 CPU sec, 2.541 real sec...
[M::process] read 676854 sequences (100000089 bp) in 1.370 CPU sec, 1.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281610, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 366, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (373.99, 166.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676736 reads in 158.132 CPU sec, 5.036 real sec
[M::mem_process_seqs] Processed 677190 reads in 96.985 CPU sec, 3.070 real sec
sorting block 118, #reads = 1063099 mem = 536878106 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281030, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 367, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (374.74, 166.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676852 sequences (100000045 bp) in 2.384 CPU sec, 2.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281513, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 367, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (373.73, 165.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676742 sequences (100000065 bp) in 1.870 CPU sec, 1.865 real sec...
[M::mem_process_seqs] Processed 677098 reads in 152.684 CPU sec, 4.833 real sec
[M::mem_process_seqs] Processed 676854 reads in 92.756 CPU sec, 2.919 real sec
sorting block 119, #reads = 1064038 mem = 536877844 peak = 1107951616
[M::process] read 677290 sequences (100000068 bp) in 2.619 CPU sec, 2.729 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281500, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 365, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (373.04, 164.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676790 sequences (100000000 bp) in 1.820 CPU sec, 1.814 real sec...
[M::mem_process_seqs] Processed 676742 reads in 85.332 CPU sec, 2.675 real sec
sorting block 120, #reads = 1063799 mem = 536879327 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280720, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 368, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (375.66, 166.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281616, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 361, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (370.27, 165.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676852 reads in 235.102 CPU sec, 7.441 real sec
[M::mem_process_seqs] Processed 677290 reads in 108.499 CPU sec, 3.431 real sec
[M::process] read 676240 sequences (100000149 bp) in 2.170 CPU sec, 2.193 real sec...
sorting block 121, #reads = 1063680 mem = 536886089 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281886, 10, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 367, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (375.54, 166.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2088, 2088, 2088)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2088, 2088)
[M::mem_pestat] mean and std.dev: (2088.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (2088, 2088)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 408, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (356.50, 142.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 676442 sequences (100000294 bp) in 2.724 CPU sec, 2.864 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281368, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 371, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (378.27, 166.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676790 reads in 162.973 CPU sec, 5.167 real sec
[M::process] read 676080 sequences (100000085 bp) in 1.847 CPU sec, 1.846 real sec...
[M::mem_process_seqs] Processed 676240 reads in 102.295 CPU sec, 3.222 real sec
sorting block 122, #reads = 1062063 mem = 536879200 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282345, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 369, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (377.32, 166.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675986 sequences (100000187 bp) in 2.449 CPU sec, 2.590 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281736, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 372, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (380.86, 168.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 291, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702)
[M::mem_pestat] mean and std.dev: (310.50, 87.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation RR
[M::process] read 676420 sequences (100000277 bp) in 1.880 CPU sec, 1.884 real sec...
[M::mem_process_seqs] Processed 676442 reads in 162.249 CPU sec, 5.136 real sec
sorting block 123, #reads = 1061748 mem = 536877971 peak = 1107951616
[M::mem_process_seqs] Processed 676080 reads in 101.562 CPU sec, 3.209 real sec
sorting block 124, #reads = 1060404 mem = 536877709 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281802, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 372, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (381.08, 169.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675952 sequences (100000205 bp) in 2.652 CPU sec, 2.756 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 280848, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 263, 592)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1406)
[M::mem_pestat] mean and std.dev: (362.57, 274.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1813)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 365, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (375.10, 166.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675782 sequences (100000076 bp) in 1.900 CPU sec, 1.898 real sec...
[M::mem_process_seqs] Processed 675986 reads in 162.169 CPU sec, 5.125 real sec
[M::mem_process_seqs] Processed 676420 reads in 101.551 CPU sec, 3.208 real sec
sorting block 125, #reads = 1061020 mem = 536879869 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280840, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 369, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (378.47, 167.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675878 sequences (100000137 bp) in 2.359 CPU sec, 2.459 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281353, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 1025, 1569)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4503)
[M::mem_pestat] mean and std.dev: (1032.90, 898.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5970)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 375, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (384.27, 170.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675650 sequences (100000094 bp) in 1.880 CPU sec, 1.882 real sec...
[M::mem_process_seqs] Processed 675952 reads in 160.607 CPU sec, 5.086 real sec
[M::mem_process_seqs] Processed 675782 reads in 99.141 CPU sec, 3.127 real sec
sorting block 126, #reads = 1059161 mem = 536880096 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281594, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 375, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (383.87, 170.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675380 sequences (100000144 bp) in 2.651 CPU sec, 2.761 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281235, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 374, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (383.75, 170.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675574 sequences (100000279 bp) in 1.880 CPU sec, 1.875 real sec...
[M::mem_process_seqs] Processed 675878 reads in 159.214 CPU sec, 5.037 real sec
[M::mem_process_seqs] Processed 675650 reads in 100.740 CPU sec, 3.173 real sec
sorting block 127, #reads = 1060272 mem = 536878218 peak = 1107951616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281443, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 377, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (387.13, 172.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678032 sequences (100000103 bp) in 2.422 CPU sec, 2.526 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281736, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 377, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (387.91, 174.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 527, 1090)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2756)
[M::mem_pestat] mean and std.dev: (656.20, 371.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3589)
[M::mem_pestat] skip orientation RR
[M::process] read 684150 sequences (100000033 bp) in 1.880 CPU sec, 1.883 real sec...
[M::mem_process_seqs] Processed 675380 reads in 162.367 CPU sec, 5.145 real sec
sorting block 128, #reads = 1059995 mem = 536882942 peak = 1107951616
[M::mem_process_seqs] Processed 675574 reads in 104.414 CPU sec, 3.286 real sec
sorting block 129, #reads = 1057671 mem = 536880037 peak = 1127137280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282618, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 360, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (373.46, 173.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682490 sequences (100000029 bp) in 2.517 CPU sec, 2.607 real sec...
[M::process] read 681720 sequences (100000290 bp) in 1.920 CPU sec, 1.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284631, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 220, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (228.30, 74.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 944)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 312, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (330.38, 157.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (279, 447, 1103)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2751)
[M::mem_pestat] mean and std.dev: (823.36, 784.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3962)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 678032 reads in 175.998 CPU sec, 5.573 real sec
[M::mem_process_seqs] Processed 684150 reads in 106.381 CPU sec, 3.374 real sec
sorting block 130, #reads = 1053455 mem = 536879969 peak = 1127137280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283782, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 323, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (338.16, 157.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680642 sequences (100000251 bp) in 2.327 CPU sec, 2.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283940, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (342.46, 157.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680090 sequences (100000100 bp) in 1.920 CPU sec, 1.927 real sec...
[M::mem_process_seqs] Processed 682490 reads in 162.292 CPU sec, 5.144 real sec
[M::mem_process_seqs] Processed 681720 reads in 100.691 CPU sec, 3.179 real sec
sorting block 131, #reads = 1061460 mem = 536878679 peak = 1127137280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283940, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 338, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (347.48, 157.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (459, 459, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (313, 678)
[M::mem_pestat] mean and std.dev: (483.33, 34.41)
[M::mem_pestat] low and high boundaries for proper pairs: (240, 751)
[M::mem_pestat] skip orientation RR
[M::process] read 679260 sequences (100000026 bp) in 2.831 CPU sec, 2.957 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283316, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (351.11, 159.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680642 reads in 160.325 CPU sec, 5.068 real sec
[M::process] read 678870 sequences (100000232 bp) in 1.870 CPU sec, 1.865 real sec...
sorting block 132, #reads = 1060334 mem = 536878192 peak = 1127137280
[M::mem_process_seqs] Processed 680090 reads in 100.499 CPU sec, 3.165 real sec
sorting block 133, #reads = 1060363 mem = 536879649 peak = 1127137280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283134, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 349, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (357.24, 160.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678110 sequences (100000126 bp) in 2.411 CPU sec, 2.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282553, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 351, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (358.57, 159.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678540 sequences (100000076 bp) in 1.860 CPU sec, 1.869 real sec...
[M::mem_process_seqs] Processed 679260 reads in 161.959 CPU sec, 5.133 real sec
[M::mem_process_seqs] Processed 678870 reads in 100.300 CPU sec, 3.156 real sec
sorting block 134, #reads = 1059597 mem = 536878257 peak = 1127137280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282528, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 358, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (366.42, 163.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678572 sequences (100000007 bp) in 2.456 CPU sec, 2.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282651, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 354, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (363.05, 163.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678092 sequences (100000232 bp) in 1.820 CPU sec, 1.817 real sec...
[M::mem_process_seqs] Processed 678110 reads in 156.747 CPU sec, 4.967 real sec
[M::mem_process_seqs] Processed 678540 reads in 99.130 CPU sec, 3.120 real sec
sorting block 135, #reads = 1059517 mem = 536880172 peak = 1127137280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282645, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 356, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (364.74, 163.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678732 sequences (100000097 bp) in 2.390 CPU sec, 2.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282361, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 363, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (370.33, 166.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677772 sequences (100000168 bp) in 1.780 CPU sec, 1.776 real sec...
[M::mem_process_seqs] Processed 678572 reads in 160.246 CPU sec, 5.076 real sec
sorting block 136, #reads = 1058946 mem = 536877726 peak = 1127137280
[M::mem_process_seqs] Processed 678092 reads in 99.505 CPU sec, 3.143 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282759, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 351, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (361.33, 164.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677396 sequences (100000152 bp) in 2.653 CPU sec, 2.737 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282600, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 353, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (362.95, 162.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 257, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (275.25, 103.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RR
[M::process] read 676498 sequences (100000130 bp) in 1.860 CPU sec, 1.859 real sec...
[M::mem_process_seqs] Processed 678732 reads in 161.091 CPU sec, 5.092 real sec
sorting block 137, #reads = 1059908 mem = 536880628 peak = 1127137280
[M::mem_process_seqs] Processed 677772 reads in 100.150 CPU sec, 3.164 real sec
sorting block 138, #reads = 1060258 mem = 536877766 peak = 1127137280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282663, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 358, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (366.87, 162.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676234 sequences (100000197 bp) in 2.379 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281381, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 365, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (373.29, 164.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676300 sequences (100000050 bp) in 1.860 CPU sec, 1.856 real sec...
[M::mem_process_seqs] Processed 677396 reads in 160.595 CPU sec, 5.092 real sec
[M::mem_process_seqs] Processed 676498 reads in 100.942 CPU sec, 3.180 real sec
sorting block 139, #reads = 1060110 mem = 536877022 peak = 1127137280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280601, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 364, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (372.48, 163.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676294 sequences (100000024 bp) in 2.364 CPU sec, 2.453 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281256, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 365, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (372.92, 163.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676140 sequences (100000160 bp) in 1.840 CPU sec, 1.838 real sec...
[M::mem_process_seqs] Processed 676234 reads in 160.605 CPU sec, 5.092 real sec
[M::mem_process_seqs] Processed 676300 reads in 99.083 CPU sec, 3.121 real sec
sorting block 140, #reads = 1059986 mem = 536879123 peak = 1127137280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280524, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 362, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (371.40, 163.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675608 sequences (100000209 bp) in 2.191 CPU sec, 2.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280830, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 360, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (370.09, 162.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675580 sequences (100000045 bp) in 1.830 CPU sec, 1.825 real sec...
[M::mem_process_seqs] Processed 676294 reads in 153.796 CPU sec, 4.874 real sec
[M::mem_process_seqs] Processed 676140 reads in 95.247 CPU sec, 3.003 real sec
sorting block 141, #reads = 1059999 mem = 536879857 peak = 1127137280
sorting block 142, #reads = 1060884 mem = 536879874 peak = 1127137280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281163, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 367, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (376.18, 164.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675096 sequences (100000143 bp) in 2.232 CPU sec, 2.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281315, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 366, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (376.46, 166.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674996 sequences (100000104 bp) in 1.820 CPU sec, 1.821 real sec...
[M::mem_process_seqs] Processed 675608 reads in 155.564 CPU sec, 4.937 real sec
[M::mem_process_seqs] Processed 675580 reads in 96.610 CPU sec, 3.043 real sec
sorting block 143, #reads = 1061312 mem = 536880725 peak = 1127137280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280786, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 376, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (385.47, 169.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674720 sequences (100000113 bp) in 2.329 CPU sec, 2.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280672, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (388.65, 172.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674696 sequences (100000118 bp) in 1.370 CPU sec, 1.376 real sec...
[M::mem_process_seqs] Processed 675096 reads in 144.638 CPU sec, 4.590 real sec
[M::mem_process_seqs] Processed 674996 reads in 85.609 CPU sec, 2.704 real sec
sorting block 144, #reads = 1062520 mem = 536879158 peak = 1127137280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280706, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (390.56, 173.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 629148 sequences (93181198 bp) in 2.404 CPU sec, 2.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281278, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (395.92, 177.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674720 reads in 135.372 CPU sec, 4.334 real sec
[M::mem_process_seqs] Processed 674696 reads in 81.934 CPU sec, 2.630 real sec
sorting block 145, #reads = 1062003 mem = 536877541 peak = 1127137280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 262266, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 380, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (391.58, 176.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 629148 reads in 70.619 CPU sec, 2.344 real sec
sorting block 146, #reads = 1062101 mem = 536879765 peak = 1127137280
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 156173916
overall: 69905739776 mem 21819.512 user 509.360 sys 731.570 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1.L004\tPU:L004\tSM:FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1\tLB:FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R1.fastq.gz FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 731.610 sec; CPU: 22328.879 sec
sorting block 147, #reads = 562019 mem = 284906787 peak = 1127137280
uniq header count 1
merging 148 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 9050271544
output reads: 156881598
bam_mem_sort: 148 calls 87.072 user 1.506 sys 89.251 real
bam_write: 148 calls 51.243 user 2.922 sys 68.907 real
execute: 1 calls 43.129 user 1.277 sys 787.169 real
merge_files: 1 calls 43.100 user 1.258 sys 55.135 real
parse_chunk: 2361 calls 101.161 user 26.710 sys 133.856 real
read_chunk: 6370 calls 43.739 user 42.157 sys 671.594 real
sort_block: 1 calls 32.625 user 41.576 sys 650.974 real
write_chunk: 4007 calls 42.766 user 0.954 sys 54.250 real
overall: 3135053824 mem 2180.008 user 91.305 sys 787.191 real
ls: cannot access '*.cram': No such file or directory