Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/c4/5f5e21edf655b2e504f4402fd26a38/1136_3U6-T1-TDNA-1_A23MHWWLT4_1/0003.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/be/d8f2c6ab74d4afaa95747355666796/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/c4/5f5e21edf655b2e504f4402fd26a38/1136_3U6-T1-TDNA-1_A23MHWWLT4_1/0003.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/be/d8f2c6ab74d4afaa95747355666796/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_3U6-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1717.604 CPU sec, 838.205 real sec
[M::process] read 682586 sequences (100000160 bp) in 0.968 CPU sec, 0.973 real sec...
[M::process] read 682834 sequences (100000105 bp) in 1.278 CPU sec, 1.283 real sec...
[M::process] read 683832 sequences (100000186 bp) in 1.450 CPU sec, 1.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 282449, 12, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (198, 273, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (337.91, 234.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (345.24, 167.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (625, 625, 1751)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4003)
[M::mem_pestat] mean and std.dev: (826.45, 602.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5129)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 367, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (72, 572)
[M::mem_pestat] mean and std.dev: (323.48, 97.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 715)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681184 sequences (100000101 bp) in 1.469 CPU sec, 1.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 282999, 7, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 310, 987)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2547)
[M::mem_pestat] mean and std.dev: (478.15, 389.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3327)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 332, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (346.84, 169.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 315, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (93, 463)
[M::mem_pestat] mean and std.dev: (274.67, 74.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 571)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283814, 5, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 213, 1090)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2892)
[M::mem_pestat] mean and std.dev: (469.94, 404.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3793)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 324, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (340.07, 167.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 354, 784)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1822)
[M::mem_pestat] mean and std.dev: (448.89, 245.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2341)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682586 reads in 238.159 CPU sec, 7.620 real sec
[M::mem_process_seqs] Processed 682834 reads in 156.513 CPU sec, 5.072 real sec
sorting block 0, #reads = 1078091 mem = 536879781 peak = 589996032
[M::process] read 682968 sequences (100000273 bp) in 1.916 CPU sec, 2.155 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282422, 8, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 317, 557)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1279)
[M::mem_pestat] mean and std.dev: (333.47, 170.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1640)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 341, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (354.54, 170.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (284, 490, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1166)
[M::mem_pestat] mean and std.dev: (483.83, 313.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1736)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681184 reads in 81.269 CPU sec, 2.569 real sec
[M::mem_process_seqs] Processed 683832 reads in 283.947 CPU sec, 9.125 real sec
sorting block 1, #reads = 1080246 mem = 536881380 peak = 706695168
[M::process] read 682390 sequences (100000028 bp) in 2.023 CPU sec, 2.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282876, 7, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 221, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (286.86, 145.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 328, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (344.11, 168.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 223, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (258.36, 101.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682046 sequences (100000044 bp) in 1.356 CPU sec, 1.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282428, 6, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 457, 929)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2343)
[M::mem_pestat] mean and std.dev: (588.39, 457.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3050)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (344.98, 167.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 340, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102)
[M::mem_pestat] mean and std.dev: (365.20, 180.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1396)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681722 sequences (100000281 bp) in 1.520 CPU sec, 1.515 real sec...
[M::mem_process_seqs] Processed 682968 reads in 167.532 CPU sec, 5.295 real sec
[M::mem_process_seqs] Processed 682390 reads in 97.944 CPU sec, 3.105 real sec
sorting block 2, #reads = 1080649 mem = 536880769 peak = 720584704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282531, 1, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 280, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171)
[M::mem_pestat] mean and std.dev: (353.66, 191.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1490)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 329, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (344.43, 166.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 322, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224)
[M::mem_pestat] mean and std.dev: (287.81, 152.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1577)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681274 sequences (100000235 bp) in 2.145 CPU sec, 2.286 real sec...
[M::process] read 681584 sequences (100000022 bp) in 1.280 CPU sec, 1.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282022, 7, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 468, 929)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2325)
[M::mem_pestat] mean and std.dev: (532.43, 385.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3023)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (345.72, 166.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 355, 2028)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5598)
[M::mem_pestat] mean and std.dev: (824.91, 826.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7383)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682046 reads in 157.564 CPU sec, 5.029 real sec
sorting block 3, #reads = 1079327 mem = 536881802 peak = 720584704
[M::mem_process_seqs] Processed 681722 reads in 98.518 CPU sec, 3.139 real sec
sorting block 4, #reads = 1080158 mem = 536877811 peak = 823611392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 281644, 1, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 278, 1040)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2706)
[M::mem_pestat] mean and std.dev: (472.78, 428.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3539)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 681240 sequences (100000236 bp) in 1.956 CPU sec, 2.080 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (209, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (344.09, 165.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 428, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072)
[M::mem_pestat] mean and std.dev: (404.28, 182.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1338)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680734 sequences (100000003 bp) in 1.400 CPU sec, 1.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 281722, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (197, 212, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 551)
[M::mem_pestat] mean and std.dev: (242.59, 94.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 669)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (343.42, 164.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 269, 734)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1764)
[M::mem_pestat] mean and std.dev: (396.00, 286.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2279)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681274 reads in 154.126 CPU sec, 4.918 real sec
[M::mem_process_seqs] Processed 681584 reads in 94.358 CPU sec, 3.013 real sec
sorting block 5, #reads = 1078418 mem = 536876174 peak = 823611392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 281927, 9, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 251, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223)
[M::mem_pestat] mean and std.dev: (360.31, 280.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1561)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (344.69, 165.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 239, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (250.44, 120.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682788 sequences (100000172 bp) in 2.599 CPU sec, 2.746 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282227, 7, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 255, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248)
[M::mem_pestat] mean and std.dev: (315.72, 172.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1605)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 337, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (352.50, 170.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 372, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (316.86, 166.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681240 reads in 152.743 CPU sec, 4.857 real sec
[M::process] read 682370 sequences (100000153 bp) in 1.808 CPU sec, 1.818 real sec...
[M::mem_process_seqs] Processed 680734 reads in 96.484 CPU sec, 3.046 real sec
sorting block 6, #reads = 1078898 mem = 536883094 peak = 823611392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282712, 1, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 364, 541)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225)
[M::mem_pestat] mean and std.dev: (378.13, 223.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1567)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (342.25, 166.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 266, 554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1296)
[M::mem_pestat] mean and std.dev: (341.00, 207.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1667)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682410 sequences (100000271 bp) in 2.500 CPU sec, 2.642 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282580, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 220, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203)
[M::mem_pestat] mean and std.dev: (308.07, 189.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1539)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (348.55, 169.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (326, 349, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (100, 665)
[M::mem_pestat] mean and std.dev: (350.60, 85.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681674 sequences (100000096 bp) in 1.680 CPU sec, 1.673 real sec...
[M::mem_process_seqs] Processed 682788 reads in 145.727 CPU sec, 4.628 real sec
[M::mem_process_seqs] Processed 682370 reads in 91.095 CPU sec, 2.884 real sec
sorting block 7, #reads = 1079153 mem = 536880070 peak = 823611392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283045, 1, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 648, 648)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1542)
[M::mem_pestat] mean and std.dev: (408.10, 241.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1989)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 333, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (347.76, 169.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 296, 1049)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2849)
[M::mem_pestat] mean and std.dev: (619.66, 607.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3749)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682452 sequences (100000161 bp) in 2.291 CPU sec, 2.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282404, 5, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (254, 254, 1211)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3125)
[M::mem_pestat] mean and std.dev: (353.00, 328.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4082)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (350.78, 170.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 436, 696)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1750)
[M::mem_pestat] mean and std.dev: (413.42, 241.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2277)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682076 sequences (100000212 bp) in 1.370 CPU sec, 1.366 real sec...
[M::mem_process_seqs] Processed 682410 reads in 143.283 CPU sec, 4.578 real sec
sorting block 8, #reads = 1081926 mem = 536878614 peak = 823611392
[M::mem_process_seqs] Processed 681674 reads in 91.391 CPU sec, 2.901 real sec
sorting block 9, #reads = 1082109 mem = 536878134 peak = 895717376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282851, 1, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 355, 680)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1684)
[M::mem_pestat] mean and std.dev: (296.75, 162.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2186)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (345.16, 168.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 163, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534)
[M::mem_pestat] mean and std.dev: (193.61, 87.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 671)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680502 sequences (100000240 bp) in 2.701 CPU sec, 2.860 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282792, 5, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 279, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (254.00, 126.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 331, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (346.45, 168.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 393, 756)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1894)
[M::mem_pestat] mean and std.dev: (455.48, 367.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2463)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682452 reads in 148.125 CPU sec, 4.699 real sec
[M::process] read 682154 sequences (100000073 bp) in 1.811 CPU sec, 1.822 real sec...
[M::mem_process_seqs] Processed 682076 reads in 92.757 CPU sec, 2.924 real sec
sorting block 10, #reads = 1080910 mem = 536880449 peak = 895717376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282322, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 172, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 365)
[M::mem_pestat] mean and std.dev: (188.09, 56.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 433)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (354.31, 169.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 421, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (336.07, 133.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682186 sequences (100000166 bp) in 2.541 CPU sec, 2.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282505, 10, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 507, 5565)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16325)
[M::mem_pestat] mean and std.dev: (1840.00, 2480.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 21705)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (345.81, 168.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 578, 1469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3979)
[M::mem_pestat] mean and std.dev: (981.80, 989.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5234)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 391, 615)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1349)
[M::mem_pestat] mean and std.dev: (395.64, 191.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1716)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681036 sequences (100000154 bp) in 1.380 CPU sec, 1.387 real sec...
[M::mem_process_seqs] Processed 680502 reads in 150.713 CPU sec, 4.799 real sec
[M::mem_process_seqs] Processed 682154 reads in 95.932 CPU sec, 3.043 real sec
sorting block 11, #reads = 1080429 mem = 536880962 peak = 895717376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282744, 2, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (308, 378, 785)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1739)
[M::mem_pestat] mean and std.dev: (538.38, 319.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2216)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 325, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (343.10, 167.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 315, 541)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1121)
[M::mem_pestat] mean and std.dev: (362.32, 172.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1411)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681726 sequences (100000006 bp) in 2.819 CPU sec, 3.004 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282150, 7, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 222, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (259.53, 136.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (349.16, 168.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 682186 reads in 142.200 CPU sec, 4.529 real sec
[M::mem_process_seqs] Processed 681036 reads in 87.436 CPU sec, 2.760 real sec
sorting block 12, #reads = 1079454 mem = 536885072 peak = 895717376
[M::process] read 681938 sequences (100000239 bp) in 2.228 CPU sec, 2.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282353, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 260, 555)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1309)
[M::mem_pestat] mean and std.dev: (339.33, 203.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1686)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 326, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (343.77, 167.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 397, 1719)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4701)
[M::mem_pestat] mean and std.dev: (859.92, 923.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6192)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683592 sequences (100000083 bp) in 2.639 CPU sec, 2.793 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281971, 7, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (346.15, 169.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 299, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (283.21, 114.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681726 reads in 136.565 CPU sec, 4.365 real sec
sorting block 13, #reads = 1079664 mem = 536884851 peak = 895717376
[M::mem_process_seqs] Processed 681938 reads in 85.992 CPU sec, 2.719 real sec
[M::process] read 683662 sequences (100000120 bp) in 2.317 CPU sec, 2.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 284040, 3, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 335, 944)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2534)
[M::mem_pestat] mean and std.dev: (470.39, 401.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3329)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 320, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (336.88, 164.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 253, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 552)
[M::mem_pestat] mean and std.dev: (271.64, 98.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 665)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 14, #reads = 1079183 mem = 536879186 peak = 895717376
[M::process] read 682280 sequences (100000209 bp) in 2.040 CPU sec, 2.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282692, 8, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 182, 929)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2505)
[M::mem_pestat] mean and std.dev: (380.81, 332.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3293)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 320, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (334.47, 161.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (120, 216, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (222.35, 106.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683592 reads in 136.816 CPU sec, 4.373 real sec
[M::mem_process_seqs] Processed 683662 reads in 86.890 CPU sec, 2.746 real sec
[M::process] read 681928 sequences (100000130 bp) in 2.200 CPU sec, 2.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283252, 7, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 212, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (220.14, 100.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 764)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 332, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (344.06, 164.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (312, 332, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (357.37, 127.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 15, #reads = 1079623 mem = 536878529 peak = 895717376
[M::process] read 682812 sequences (100000015 bp) in 2.230 CPU sec, 2.358 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282603, 3, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 259, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290)
[M::mem_pestat] mean and std.dev: (353.46, 276.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1671)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (347.02, 165.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 313, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (340.92, 141.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682280 reads in 133.536 CPU sec, 4.266 real sec
[M::mem_process_seqs] Processed 681928 reads in 84.543 CPU sec, 2.674 real sec
sorting block 16, #reads = 1079613 mem = 536879800 peak = 895717376
[M::process] read 682530 sequences (100000293 bp) in 2.257 CPU sec, 2.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282089, 4, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 222, 830)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2188)
[M::mem_pestat] mean and std.dev: (382.00, 331.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2867)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 327, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (341.53, 165.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 276, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (317.40, 144.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680778 sequences (100000218 bp) in 1.974 CPU sec, 2.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282264, 6, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (246.10, 95.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (343.35, 166.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 311, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (269.00, 77.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682812 reads in 136.859 CPU sec, 4.369 real sec
[M::mem_process_seqs] Processed 682530 reads in 86.612 CPU sec, 2.736 real sec
sorting block 17, #reads = 1080263 mem = 536880161 peak = 895717376
[M::process] read 682162 sequences (100000284 bp) in 2.201 CPU sec, 2.327 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282293, 3, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 244, 572)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1326)
[M::mem_pestat] mean and std.dev: (360.62, 259.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1703)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 341, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (352.57, 166.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (296, 395, 1735)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4613)
[M::mem_pestat] mean and std.dev: (1076.00, 999.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6052)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 18, #reads = 1079991 mem = 536886213 peak = 895717376
[M::process] read 682116 sequences (100000186 bp) in 2.299 CPU sec, 2.415 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282425, 6, 19)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 329, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (344.43, 166.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 220, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 416)
[M::mem_pestat] mean and std.dev: (207.75, 62.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 486)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 680778 reads in 137.015 CPU sec, 4.370 real sec
[M::mem_process_seqs] Processed 682162 reads in 87.048 CPU sec, 2.747 real sec
[M::process] read 682260 sequences (100000245 bp) in 2.244 CPU sec, 2.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282082, 1, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 266, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (329.33, 183.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (343.82, 166.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (373.00, 144.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 19, #reads = 1079217 mem = 536878442 peak = 895717376
[M::process] read 682058 sequences (100000139 bp) in 1.962 CPU sec, 2.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282169, 8, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 251, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (249.33, 149.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1344)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 324, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (341.00, 165.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (281, 368, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1301)
[M::mem_pestat] mean and std.dev: (397.10, 247.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1641)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682116 reads in 140.238 CPU sec, 4.466 real sec
[M::mem_process_seqs] Processed 682260 reads in 87.785 CPU sec, 2.764 real sec
[M::process] read 681988 sequences (100000087 bp) in 1.598 CPU sec, 1.654 real sec...
sorting block 20, #reads = 1080486 mem = 536879303 peak = 895717376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282004, 3, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 301, 1393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3771)
[M::mem_pestat] mean and std.dev: (488.08, 472.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4960)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 326, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (342.24, 166.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 337, 565)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203)
[M::mem_pestat] mean and std.dev: (377.60, 233.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1522)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682610 sequences (100000073 bp) in 2.202 CPU sec, 2.343 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282678, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 400, 582)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1262)
[M::mem_pestat] mean and std.dev: (350.59, 140.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1602)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 328, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (345.21, 168.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 682058 reads in 136.993 CPU sec, 4.363 real sec
[M::mem_process_seqs] Processed 681988 reads in 85.277 CPU sec, 2.685 real sec
[M::process] read 681298 sequences (100000137 bp) in 1.715 CPU sec, 1.768 real sec...
sorting block 21, #reads = 1080076 mem = 536881080 peak = 895717376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282515, 7, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 227, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (252.08, 126.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 325, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (341.32, 165.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 245, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (288.32, 155.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682168 sequences (100000161 bp) in 2.113 CPU sec, 2.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 281846, 2, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 354, 720)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1742)
[M::mem_pestat] mean and std.dev: (375.73, 230.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2253)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 332, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (343.84, 161.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 270, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1254)
[M::mem_pestat] mean and std.dev: (400.82, 270.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1592)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682610 reads in 134.960 CPU sec, 4.290 real sec
[M::process] read 680524 sequences (100000209 bp) in 1.491 CPU sec, 1.507 real sec...
sorting block 22, #reads = 1080096 mem = 536882077 peak = 895717376
[M::mem_process_seqs] Processed 681298 reads in 85.021 CPU sec, 2.677 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 281976, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 229, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1456)
[M::mem_pestat] mean and std.dev: (357.19, 272.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1879)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (339.31, 161.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 171, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408)
[M::mem_pestat] mean and std.dev: (167.77, 62.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 502)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 23, #reads = 1079662 mem = 536878391 peak = 946089984
[M::process] read 683030 sequences (100000157 bp) in 2.277 CPU sec, 2.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 281393, 0, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 232, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (97, 407)
[M::mem_pestat] mean and std.dev: (220.14, 42.60)
[M::mem_pestat] low and high boundaries for proper pairs: (35, 469)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 342, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (353.50, 166.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 452, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (366.76, 124.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683178 sequences (100000027 bp) in 1.410 CPU sec, 1.414 real sec...
[M::mem_process_seqs] Processed 682168 reads in 133.593 CPU sec, 4.239 real sec
[M::mem_process_seqs] Processed 680524 reads in 83.920 CPU sec, 2.648 real sec
sorting block 24, #reads = 1080361 mem = 536880269 peak = 946089984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282368, 6, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 410, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1304)
[M::mem_pestat] mean and std.dev: (321.10, 166.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1682)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (340.63, 164.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 249, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1326)
[M::mem_pestat] mean and std.dev: (339.15, 274.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1711)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682114 sequences (100000226 bp) in 2.291 CPU sec, 2.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 281948, 8, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 233, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (256.55, 149.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 323, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (338.16, 164.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 205, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (257.69, 163.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681668 sequences (100000277 bp) in 1.420 CPU sec, 1.427 real sec...
[M::mem_process_seqs] Processed 683030 reads in 135.935 CPU sec, 4.311 real sec
[M::mem_process_seqs] Processed 683178 reads in 86.006 CPU sec, 2.721 real sec
sorting block 25, #reads = 1080166 mem = 536879625 peak = 946089984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 281973, 5, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (255, 327, 571)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203)
[M::mem_pestat] mean and std.dev: (362.59, 170.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1519)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (343.85, 165.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 364, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (339.07, 166.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681280 sequences (100000071 bp) in 2.354 CPU sec, 2.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282083, 7, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 197, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614)
[M::mem_pestat] mean and std.dev: (232.35, 93.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 332, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (346.29, 165.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 300, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (84, 464)
[M::mem_pestat] mean and std.dev: (284.64, 67.71)
[M::mem_pestat] low and high boundaries for proper pairs: (8, 555)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682084 sequences (100000267 bp) in 1.470 CPU sec, 1.466 real sec...
[M::mem_process_seqs] Processed 682114 reads in 138.012 CPU sec, 4.376 real sec
[M::mem_process_seqs] Processed 681668 reads in 86.168 CPU sec, 2.722 real sec
sorting block 26, #reads = 1079571 mem = 536876603 peak = 946089984
[M::process] read 682564 sequences (100000213 bp) in 2.456 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282350, 7, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 296, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (392.36, 209.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 326, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (341.46, 164.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 480, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1268)
[M::mem_pestat] mean and std.dev: (323.82, 170.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1639)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681976 sequences (100000204 bp) in 1.480 CPU sec, 1.476 real sec...
[M::mem_process_seqs] Processed 682084 reads in 81.356 CPU sec, 2.571 real sec
sorting block 27, #reads = 1080301 mem = 536878541 peak = 946089984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 281483, 7, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 352, 848)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2108)
[M::mem_pestat] mean and std.dev: (548.00, 553.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2763)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (348.59, 166.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 309, 659)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1519)
[M::mem_pestat] mean and std.dev: (350.68, 180.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1949)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282624, 7, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (289, 339, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (349.76, 113.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 937)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (338.88, 164.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 319, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (298.58, 137.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1335)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681280 reads in 217.484 CPU sec, 6.913 real sec
[M::mem_process_seqs] Processed 682564 reads in 101.762 CPU sec, 3.232 real sec
[M::process] read 683986 sequences (100000218 bp) in 1.945 CPU sec, 1.972 real sec...
sorting block 28, #reads = 1078890 mem = 536878701 peak = 1035714560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 281973, 5, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (327, 331, 566)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (350.20, 160.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 325, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (341.94, 165.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 330, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (281.90, 100.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684760 sequences (100000213 bp) in 2.507 CPU sec, 2.662 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283383, 3, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 199, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1236)
[M::mem_pestat] mean and std.dev: (281.35, 212.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1599)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 317, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (336.68, 167.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 265, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083)
[M::mem_pestat] mean and std.dev: (342.74, 234.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1361)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681976 reads in 138.962 CPU sec, 4.412 real sec
[M::process] read 686152 sequences (100000044 bp) in 1.554 CPU sec, 1.570 real sec...
sorting block 29, #reads = 1079333 mem = 536879250 peak = 1035714560
[M::mem_process_seqs] Processed 683986 reads in 87.936 CPU sec, 2.775 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284066, 9, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 235, 571)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1437)
[M::mem_pestat] mean and std.dev: (405.45, 352.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1870)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 321, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (341.17, 171.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 292, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (322.43, 141.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 953)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 30, #reads = 1078475 mem = 536878684 peak = 1035714560
[M::process] read 684406 sequences (100000146 bp) in 2.181 CPU sec, 2.316 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284754, 10, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (339, 346, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660)
[M::mem_pestat] mean and std.dev: (376.89, 126.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 310, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (330.67, 166.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 662, 908)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2404)
[M::mem_pestat] mean and std.dev: (568.80, 342.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3152)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 269, 654)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1668)
[M::mem_pestat] mean and std.dev: (306.09, 235.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2175)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 685640 sequences (100000010 bp) in 1.490 CPU sec, 1.490 real sec...
[M::mem_process_seqs] Processed 684760 reads in 142.749 CPU sec, 4.535 real sec
[M::mem_process_seqs] Processed 686152 reads in 89.877 CPU sec, 2.835 real sec
sorting block 31, #reads = 1077925 mem = 536880368 peak = 1035714560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283523, 1, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 199, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (249.94, 157.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 321, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (338.29, 167.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 347, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (60, 615)
[M::mem_pestat] mean and std.dev: (306.55, 80.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684446 sequences (100000151 bp) in 2.492 CPU sec, 2.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 283943, 2, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 265, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1584)
[M::mem_pestat] mean and std.dev: (416.74, 327.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2048)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 310, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (329.92, 164.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 326, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204)
[M::mem_pestat] mean and std.dev: (357.47, 237.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1528)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683042 sequences (100000006 bp) in 1.470 CPU sec, 1.475 real sec...
[M::mem_process_seqs] Processed 684406 reads in 142.102 CPU sec, 4.504 real sec
[M::mem_process_seqs] Processed 685640 reads in 90.443 CPU sec, 2.852 real sec
sorting block 32, #reads = 1078219 mem = 536878784 peak = 1035714560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283523, 9, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 431, 775)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1875)
[M::mem_pestat] mean and std.dev: (435.73, 244.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2425)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 320, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (338.12, 167.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 248, 865)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2321)
[M::mem_pestat] mean and std.dev: (317.92, 273.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3049)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682352 sequences (100000288 bp) in 2.483 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283292, 5, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 239, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (259.48, 114.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 330, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (345.60, 168.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 308, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066)
[M::mem_pestat] mean and std.dev: (267.70, 112.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1355)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681750 sequences (100000115 bp) in 1.480 CPU sec, 1.480 real sec...
[M::mem_process_seqs] Processed 684446 reads in 145.176 CPU sec, 4.602 real sec
[M::mem_process_seqs] Processed 683042 reads in 90.912 CPU sec, 2.873 real sec
sorting block 33, #reads = 1076694 mem = 536878419 peak = 1035714560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282908, 1, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (291, 540, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230)
[M::mem_pestat] mean and std.dev: (407.14, 147.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1543)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (343.52, 165.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 235, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (242.48, 76.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682758 sequences (100000301 bp) in 2.494 CPU sec, 2.632 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 282765, 2, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (334, 456, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1162)
[M::mem_pestat] mean and std.dev: (480.05, 308.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1713)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (348.18, 167.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 353, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (306.57, 109.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681946 sequences (100000003 bp) in 1.490 CPU sec, 1.491 real sec...
[M::mem_process_seqs] Processed 682352 reads in 146.353 CPU sec, 4.643 real sec
sorting block 34, #reads = 1076266 mem = 536878146 peak = 1035714560
[M::mem_process_seqs] Processed 681750 reads in 92.702 CPU sec, 2.931 real sec
sorting block 35, #reads = 1075941 mem = 536878816 peak = 1063997440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282622, 3, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 239, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834)
[M::mem_pestat] mean and std.dev: (260.26, 120.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1056)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 324, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (340.22, 164.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 477, 1753)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4949)
[M::mem_pestat] mean and std.dev: (789.52, 794.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6547)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684902 sequences (100000182 bp) in 2.476 CPU sec, 2.598 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282967, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 236, 1258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3312)
[M::mem_pestat] mean and std.dev: (549.75, 456.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4339)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 329, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (346.07, 168.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 302, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116)
[M::mem_pestat] mean and std.dev: (316.71, 185.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1438)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688576 sequences (100000241 bp) in 1.540 CPU sec, 1.541 real sec...
[M::mem_process_seqs] Processed 682758 reads in 145.363 CPU sec, 4.607 real sec
[M::mem_process_seqs] Processed 681946 reads in 90.016 CPU sec, 2.842 real sec
sorting block 36, #reads = 1075156 mem = 536879696 peak = 1063997440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284870, 7, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 294, 820)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2060)
[M::mem_pestat] mean and std.dev: (482.30, 334.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2680)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 316, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (335.87, 167.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 203, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547)
[M::mem_pestat] mean and std.dev: (181.40, 61.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 679)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686794 sequences (100000170 bp) in 2.429 CPU sec, 2.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 285733, 2, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 343, 705)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1723)
[M::mem_pestat] mean and std.dev: (302.36, 163.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2232)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 297, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (316.85, 158.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 220, 855)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2175)
[M::mem_pestat] mean and std.dev: (413.31, 330.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2835)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685502 sequences (100000153 bp) in 1.490 CPU sec, 1.490 real sec...
[M::mem_process_seqs] Processed 684902 reads in 152.830 CPU sec, 4.854 real sec
[M::mem_process_seqs] Processed 688576 reads in 98.694 CPU sec, 3.120 real sec
sorting block 37, #reads = 1076385 mem = 536881044 peak = 1063997440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284753, 6, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 285, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (302.59, 172.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 302, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (321.29, 159.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 241, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (252.94, 85.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686390 sequences (100000050 bp) in 2.321 CPU sec, 2.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 284551, 4, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (316, 440, 3015)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8413)
[M::mem_pestat] mean and std.dev: (1377.47, 1598.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11112)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 312, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (328.70, 161.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 332, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174)
[M::mem_pestat] mean and std.dev: (388.52, 247.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1493)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683676 sequences (100000009 bp) in 1.480 CPU sec, 1.474 real sec...
[M::mem_process_seqs] Processed 686794 reads in 146.945 CPU sec, 4.665 real sec
sorting block 38, #reads = 1073062 mem = 536881171 peak = 1063997440
[M::mem_process_seqs] Processed 685502 reads in 91.341 CPU sec, 2.885 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284792, 6, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 232, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (256.76, 160.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 306, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (324.60, 161.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 232, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (214.79, 76.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 39, #reads = 1075245 mem = 536879403 peak = 1063997440
[M::process] read 683756 sequences (100000018 bp) in 2.516 CPU sec, 2.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283693, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 547, 917)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2337)
[M::mem_pestat] mean and std.dev: (541.40, 287.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3047)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 327, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (342.62, 168.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 304, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (373.35, 224.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682552 sequences (100000043 bp) in 1.490 CPU sec, 1.487 real sec...
[M::mem_process_seqs] Processed 686390 reads in 145.207 CPU sec, 4.604 real sec
[M::mem_process_seqs] Processed 683676 reads in 92.794 CPU sec, 2.936 real sec
sorting block 40, #reads = 1076378 mem = 536877952 peak = 1063997440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283584, 5, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 363, 703)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1665)
[M::mem_pestat] mean and std.dev: (385.05, 276.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2146)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (340.99, 168.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 326, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (308.79, 128.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682078 sequences (100000144 bp) in 2.523 CPU sec, 2.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282938, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 249, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (224.89, 78.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 329, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (345.48, 169.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 344, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104)
[M::mem_pestat] mean and std.dev: (331.06, 202.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1417)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682800 sequences (100000285 bp) in 1.470 CPU sec, 1.475 real sec...
[M::mem_process_seqs] Processed 683756 reads in 143.728 CPU sec, 4.562 real sec
[M::mem_process_seqs] Processed 682552 reads in 91.374 CPU sec, 2.885 real sec
sorting block 41, #reads = 1074928 mem = 536878208 peak = 1063997440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283147, 9, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 448, 570)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1252)
[M::mem_pestat] mean and std.dev: (390.92, 206.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1593)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 333, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (350.17, 171.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 287, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (88, 523)
[M::mem_pestat] mean and std.dev: (277.68, 68.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 610)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683168 sequences (100000145 bp) in 2.480 CPU sec, 2.560 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283341, 5, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 449, 865)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2179)
[M::mem_pestat] mean and std.dev: (399.94, 259.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2836)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 329, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (347.54, 172.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 272, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075)
[M::mem_pestat] mean and std.dev: (300.42, 126.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1349)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686878 sequences (100000040 bp) in 1.460 CPU sec, 1.466 real sec...
[M::mem_process_seqs] Processed 682078 reads in 141.919 CPU sec, 4.497 real sec
[M::mem_process_seqs] Processed 682800 reads in 89.552 CPU sec, 2.829 real sec
sorting block 42, #reads = 1074828 mem = 536883851 peak = 1063997440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283818, 2, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 266, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (297.59, 204.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 324, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (345.01, 172.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 486, 1277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3347)
[M::mem_pestat] mean and std.dev: (545.62, 421.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4382)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685034 sequences (100000023 bp) in 2.341 CPU sec, 2.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 284377, 7, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (264, 340, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (361.67, 142.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 305, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (324.08, 161.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (313, 636, 943)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2203)
[M::mem_pestat] mean and std.dev: (693.95, 432.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2833)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683134 sequences (100000074 bp) in 1.530 CPU sec, 1.522 real sec...
[M::mem_process_seqs] Processed 683168 reads in 148.892 CPU sec, 4.728 real sec
sorting block 43, #reads = 1076188 mem = 536879424 peak = 1063997440
[M::mem_process_seqs] Processed 686878 reads in 90.639 CPU sec, 2.861 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284020, 0, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (260, 365, 661)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1463)
[M::mem_pestat] mean and std.dev: (453.27, 243.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1864)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 315, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (331.76, 163.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 276, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282)
[M::mem_pestat] mean and std.dev: (360.07, 237.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1653)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 44, #reads = 1073238 mem = 536879631 peak = 1063997440
[M::process] read 686062 sequences (100000231 bp) in 2.237 CPU sec, 2.345 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283244, 8, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 284, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (271.59, 96.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (340.68, 164.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 305, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849)
[M::mem_pestat] mean and std.dev: (256.36, 83.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685034 reads in 136.391 CPU sec, 4.333 real sec
[M::process] read 685102 sequences (100000028 bp) in 1.490 CPU sec, 1.497 real sec...
[M::mem_process_seqs] Processed 683134 reads in 86.536 CPU sec, 2.729 real sec
sorting block 45, #reads = 1079216 mem = 536879471 peak = 1063997440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 284343, 2, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 340, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099)
[M::mem_pestat] mean and std.dev: (334.63, 136.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1395)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 306, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (323.71, 158.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 380, 928)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2392)
[M::mem_pestat] mean and std.dev: (601.50, 525.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3124)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683668 sequences (100000036 bp) in 2.506 CPU sec, 2.626 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284481, 15, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (331, 331, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (103, 673)
[M::mem_pestat] mean and std.dev: (319.09, 112.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 311, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (327.19, 159.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 241, 1081)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2809)
[M::mem_pestat] mean and std.dev: (660.73, 616.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3673)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 312, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (291.10, 107.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 684220 sequences (100000110 bp) in 1.430 CPU sec, 1.437 real sec...
[M::mem_process_seqs] Processed 686062 reads in 140.422 CPU sec, 4.454 real sec
[M::mem_process_seqs] Processed 685102 reads in 88.263 CPU sec, 2.790 real sec
sorting block 46, #reads = 1077368 mem = 536878075 peak = 1063997440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283639, 8, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 496, 570)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1432)
[M::mem_pestat] mean and std.dev: (399.15, 217.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1863)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 325, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (339.20, 164.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 313, 729)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1731)
[M::mem_pestat] mean and std.dev: (464.83, 281.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2232)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683714 sequences (100000018 bp) in 2.522 CPU sec, 2.640 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283758, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (261, 496, 866)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2076)
[M::mem_pestat] mean and std.dev: (533.79, 317.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2681)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 317, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (332.49, 161.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 242, 262)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (97, 372)
[M::mem_pestat] mean and std.dev: (226.00, 52.30)
[M::mem_pestat] low and high boundaries for proper pairs: (17, 435)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683650 sequences (100000151 bp) in 1.475 CPU sec, 1.479 real sec...
[M::mem_process_seqs] Processed 683668 reads in 142.064 CPU sec, 4.505 real sec
sorting block 47, #reads = 1078438 mem = 536878616 peak = 1063997440
[M::mem_process_seqs] Processed 684220 reads in 90.483 CPU sec, 2.858 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283219, 8, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 500, 1069)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2763)
[M::mem_pestat] mean and std.dev: (587.97, 396.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3610)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (336.71, 163.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 386, 624)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1558)
[M::mem_pestat] mean and std.dev: (387.76, 229.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2025)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 48, #reads = 1077011 mem = 536880412 peak = 1063997440
[M::process] read 683576 sequences (100000251 bp) in 2.539 CPU sec, 2.649 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 283540, 4, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 266, 728)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1840)
[M::mem_pestat] mean and std.dev: (493.33, 435.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2396)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 324, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (340.41, 166.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 309, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (294.56, 124.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684846 sequences (100000255 bp) in 1.530 CPU sec, 1.531 real sec...
[M::mem_process_seqs] Processed 683714 reads in 143.112 CPU sec, 4.536 real sec
[M::mem_process_seqs] Processed 683650 reads in 91.134 CPU sec, 2.876 real sec
sorting block 49, #reads = 1076789 mem = 536882003 peak = 1063997440
[M::process] read 688992 sequences (100000232 bp) in 2.747 CPU sec, 2.828 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 284128, 8, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 260, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (274.97, 120.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 311, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (332.73, 166.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 211, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (211.88, 72.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687468 sequences (100000231 bp) in 1.520 CPU sec, 1.519 real sec...
[M::mem_process_seqs] Processed 684846 reads in 85.264 CPU sec, 2.677 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283688, 6, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (249, 331, 627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1383)
[M::mem_pestat] mean and std.dev: (427.10, 234.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1761)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 323, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (340.41, 167.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 351, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (372.12, 163.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 285620, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 253, 1373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3705)
[M::mem_pestat] mean and std.dev: (442.89, 488.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4871)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 297, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (314.24, 154.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (299, 324, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (247, 377)
[M::mem_pestat] mean and std.dev: (318.25, 11.12)
[M::mem_pestat] low and high boundaries for proper pairs: (221, 403)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687570 sequences (100000155 bp) in 1.989 CPU sec, 1.991 real sec...
[M::mem_process_seqs] Processed 683576 reads in 235.062 CPU sec, 7.437 real sec
sorting block 50, #reads = 1072042 mem = 536881288 peak = 1063997440
[M::mem_process_seqs] Processed 688992 reads in 114.122 CPU sec, 3.621 real sec
sorting block 51, #reads = 1073969 mem = 536879731 peak = 1091641344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 285123, 10, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 287, 1027)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2615)
[M::mem_pestat] mean and std.dev: (479.29, 333.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3409)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 303, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (318.17, 154.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (69, 328, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (240.60, 130.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (289, 362, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 619)
[M::mem_pestat] mean and std.dev: (307.95, 111.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 688036 sequences (100000109 bp) in 2.837 CPU sec, 2.976 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 284877, 5, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (251, 265, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (294.68, 78.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 303, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (320.73, 157.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (317.30, 142.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685314 sequences (100000287 bp) in 1.500 CPU sec, 1.504 real sec...
[M::mem_process_seqs] Processed 687468 reads in 151.507 CPU sec, 4.799 real sec
[M::mem_process_seqs] Processed 687570 reads in 94.858 CPU sec, 2.995 real sec
sorting block 52, #reads = 1072321 mem = 536880717 peak = 1091641344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 285104, 2, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (125, 225, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524)
[M::mem_pestat] mean and std.dev: (177.00, 56.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 657)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 293, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (311.33, 151.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 200, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (207.62, 76.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 773)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685680 sequences (100000216 bp) in 2.408 CPU sec, 2.548 real sec...
[M::process] read 684490 sequences (100000137 bp) in 1.480 CPU sec, 1.484 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 284013, 6, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 559, 969)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2629)
[M::mem_pestat] mean and std.dev: (740.00, 778.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3853)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 312, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (325.90, 156.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 177, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (203.29, 70.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688036 reads in 147.667 CPU sec, 4.683 real sec
[M::mem_process_seqs] Processed 685314 reads in 92.269 CPU sec, 2.920 real sec
sorting block 53, #reads = 1075504 mem = 536877680 peak = 1091641344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283271, 5, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 281, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127)
[M::mem_pestat] mean and std.dev: (356.16, 188.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1434)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 309, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (323.91, 156.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 274, 749)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1789)
[M::mem_pestat] mean and std.dev: (511.89, 472.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2404)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684236 sequences (100000004 bp) in 2.508 CPU sec, 2.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283687, 4, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 180, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (240.68, 132.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 313, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (328.41, 158.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 323, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098)
[M::mem_pestat] mean and std.dev: (316.83, 113.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1399)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683708 sequences (100000209 bp) in 1.490 CPU sec, 1.488 real sec...
[M::mem_process_seqs] Processed 685680 reads in 146.360 CPU sec, 4.644 real sec
sorting block 54, #reads = 1075522 mem = 536882187 peak = 1091641344
[M::mem_process_seqs] Processed 684490 reads in 91.437 CPU sec, 2.893 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283197, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 198, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464)
[M::mem_pestat] mean and std.dev: (197.17, 61.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 566)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 317, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (332.54, 161.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 294, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (224.40, 101.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 906)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 55, #reads = 1076103 mem = 536878979 peak = 1091641344
[M::process] read 609122 sequences (89080147 bp) in 2.336 CPU sec, 2.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283117, 1, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (237, 376, 581)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269)
[M::mem_pestat] mean and std.dev: (350.05, 150.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1613)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 318, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (335.13, 163.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (325, 459, 730)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1540)
[M::mem_pestat] mean and std.dev: (477.17, 272.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1945)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684236 reads in 143.777 CPU sec, 4.616 real sec
[M::mem_process_seqs] Processed 683708 reads in 89.564 CPU sec, 2.879 real sec
sorting block 56, #reads = 1077063 mem = 536880624 peak = 1091641344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 252782, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 150, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (225.54, 143.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 317, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (336.80, 166.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 394, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217)
[M::mem_pestat] mean and std.dev: (352.94, 159.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1558)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 609122 reads in 69.367 CPU sec, 2.290 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 62106298
overall: 69883166720 mem 8575.283 user 297.127 sys 1072.946 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_3U6-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0003.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1072.980 sec; CPU: 8872.413 sec
sorting block 57, #reads = 991182 mem = 494158265 peak = 1091641344
uniq header count 1
merging 58 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3649666861
output reads: 62437720
bam_mem_sort: 58 calls 28.256 user 0.284 sys 28.607 real
bam_write: 58 calls 11.842 user 0.475 sys 12.277 real
execute: 1 calls 12.776 user 1.004 sys 1094.250 real
merge_files: 1 calls 12.760 user 0.982 sys 20.065 real
parse_chunk: 927 calls 40.384 user 9.227 sys 50.170 real
read_chunk: 2502 calls 17.496 user 18.982 sys 234.111 real
sort_block: 1 calls 13.499 user 18.546 sys 227.366 real
write_chunk: 1573 calls 12.395 user 0.754 sys 19.257 real
overall: 2684489728 mem 781.299 user 35.994 sys 1094.269 real
ls: cannot access '*.cram': No such file or directory