This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_bR4-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1967.662 CPU sec, 96.340 real sec
[M::process] read 679546 sequences (100000252 bp) in 1.708 CPU sec, 1.710 real sec...
[M::process] read 678804 sequences (100000105 bp) in 2.171 CPU sec, 2.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282310, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 314, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (331.43, 150.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677972 sequences (100000179 bp) in 2.142 CPU sec, 2.736 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282170, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677494 sequences (100000161 bp) in 2.101 CPU sec, 2.389 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (219, 318, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (335.39, 151.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1079)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679546 reads in 205.728 CPU sec, 8.936 real sec
[M::mem_process_seqs] Processed 678804 reads in 128.554 CPU sec, 5.700 real sec
sorting block 0, #reads = 1080776 mem = 536885824 peak = 539549696
[M::process] read 677210 sequences (100000071 bp) in 2.990 CPU sec, 3.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281557, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 328, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (343.55, 153.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677084 sequences (100000135 bp) in 2.246 CPU sec, 2.333 real sec...
[M::mem_process_seqs] Processed 677494 reads in 111.614 CPU sec, 4.258 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282102, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 325, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (341.13, 152.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281345, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 332, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (346.48, 153.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677972 reads in 306.334 CPU sec, 11.558 real sec
[M::mem_process_seqs] Processed 677210 reads in 148.944 CPU sec, 6.066 real sec
sorting block 1, #reads = 1080782 mem = 536879546 peak = 675536896
[M::process] read 677040 sequences (100000016 bp) in 2.446 CPU sec, 2.705 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281812, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 335, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (349.20, 154.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676780 sequences (100000257 bp) in 3.103 CPU sec, 3.683 real sec...
sorting block 2, #reads = 1080252 mem = 536880067 peak = 826552320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281618, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 337, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (350.54, 154.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677084 reads in 194.800 CPU sec, 7.251 real sec
[M::mem_process_seqs] Processed 677040 reads in 130.164 CPU sec, 5.099 real sec
[M::process] read 676790 sequences (100000179 bp) in 2.759 CPU sec, 3.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281783, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 337, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (349.99, 153.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 3, #reads = 1079351 mem = 536885286 peak = 826552320
[M::process] read 676524 sequences (100000238 bp) in 3.008 CPU sec, 3.805 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281292, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 340, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (352.80, 155.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676780 reads in 195.170 CPU sec, 7.510 real sec
[M::mem_process_seqs] Processed 676790 reads in 131.658 CPU sec, 5.363 real sec
[M::process] read 676348 sequences (100000089 bp) in 2.734 CPU sec, 3.432 real sec...
sorting block 4, #reads = 1079629 mem = 536880016 peak = 826552320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281200, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 342, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (354.70, 156.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281690, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 342, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (355.38, 156.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676200 sequences (100000259 bp) in 3.075 CPU sec, 3.922 real sec...
[M::mem_process_seqs] Processed 676524 reads in 195.054 CPU sec, 7.544 real sec
[M::mem_process_seqs] Processed 676348 reads in 131.851 CPU sec, 5.158 real sec
sorting block 5, #reads = 1079937 mem = 536880642 peak = 826552320
[M::process] read 675752 sequences (100000064 bp) in 3.183 CPU sec, 3.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281553, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 343, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (356.49, 155.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676200 reads in 108.803 CPU sec, 4.300 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281165, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (357.52, 155.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675914 sequences (100000182 bp) in 3.573 CPU sec, 4.696 real sec...
[M::mem_process_seqs] Processed 675752 reads in 110.343 CPU sec, 4.470 real sec
sorting block 6, #reads = 1080248 mem = 536878499 peak = 826552320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281665, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (358.22, 155.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675558 sequences (100000178 bp) in 2.873 CPU sec, 3.550 real sec...
[M::mem_process_seqs] Processed 675914 reads in 105.667 CPU sec, 4.472 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281027, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (359.75, 156.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675328 sequences (100000202 bp) in 3.317 CPU sec, 3.959 real sec...
sorting block 7, #reads = 1080160 mem = 536877631 peak = 826552320
[M::mem_process_seqs] Processed 675558 reads in 108.583 CPU sec, 4.534 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281539, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (359.63, 155.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675438 sequences (100000247 bp) in 3.249 CPU sec, 3.863 real sec...
[M::mem_process_seqs] Processed 675328 reads in 107.955 CPU sec, 4.630 real sec
sorting block 8, #reads = 1079787 mem = 536881017 peak = 826552320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281248, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (360.32, 156.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675592 sequences (100000149 bp) in 3.184 CPU sec, 3.976 real sec...
[M::mem_process_seqs] Processed 675438 reads in 108.676 CPU sec, 4.433 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280949, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (360.43, 156.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675258 sequences (100000077 bp) in 3.273 CPU sec, 3.887 real sec...
[M::mem_process_seqs] Processed 675592 reads in 110.582 CPU sec, 4.527 real sec
sorting block 9, #reads = 1078966 mem = 536877313 peak = 826552320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280745, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 350, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (363.41, 157.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675114 sequences (100000067 bp) in 3.255 CPU sec, 3.843 real sec...
[M::mem_process_seqs] Processed 675258 reads in 112.260 CPU sec, 4.638 real sec
sorting block 10, #reads = 1078694 mem = 536878663 peak = 826552320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281650, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (364.40, 158.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675002 sequences (100000103 bp) in 2.998 CPU sec, 3.989 real sec...
[M::mem_process_seqs] Processed 675114 reads in 109.707 CPU sec, 4.167 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280664, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (363.43, 157.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675002 reads in 111.188 CPU sec, 4.208 real sec
[M::process] read 674880 sequences (100000230 bp) in 3.768 CPU sec, 4.355 real sec...
sorting block 11, #reads = 1079354 mem = 536876806 peak = 826552320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280891, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 350, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (362.54, 155.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674832 sequences (100000052 bp) in 3.320 CPU sec, 3.764 real sec...
[M::mem_process_seqs] Processed 674880 reads in 109.869 CPU sec, 3.972 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281208, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 350, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (362.78, 155.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674598 sequences (100000081 bp) in 3.414 CPU sec, 3.777 real sec...
sorting block 12, #reads = 1079973 mem = 536880363 peak = 826552320
[M::mem_process_seqs] Processed 674832 reads in 114.599 CPU sec, 3.969 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281257, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 351, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (363.21, 155.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674598 reads in 111.364 CPU sec, 3.839 real sec
[M::process] read 674600 sequences (100000265 bp) in 3.707 CPU sec, 4.225 real sec...
sorting block 13, #reads = 1078736 mem = 536878868 peak = 826552320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280606, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 346, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (358.33, 152.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674410 sequences (100000014 bp) in 3.023 CPU sec, 3.246 real sec...
[M::mem_process_seqs] Processed 674600 reads in 110.697 CPU sec, 3.814 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280902, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 348, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (360.85, 153.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674170 sequences (100000050 bp) in 2.892 CPU sec, 3.239 real sec...
sorting block 14, #reads = 1078511 mem = 536877548 peak = 826552320
[M::mem_process_seqs] Processed 674410 reads in 108.357 CPU sec, 3.688 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281083, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 350, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (362.54, 154.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674458 sequences (100000181 bp) in 2.989 CPU sec, 3.358 real sec...
[M::mem_process_seqs] Processed 674170 reads in 105.582 CPU sec, 3.582 real sec
sorting block 15, #reads = 1078273 mem = 536879130 peak = 826552320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281631, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 351, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (362.92, 154.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674464 sequences (100000026 bp) in 2.967 CPU sec, 3.168 real sec...
[M::mem_process_seqs] Processed 674458 reads in 105.890 CPU sec, 3.539 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281262, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (361.69, 154.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1079172 mem = 536879385 peak = 826552320
[M::process] read 674708 sequences (100000118 bp) in 3.316 CPU sec, 3.554 real sec...
[M::mem_process_seqs] Processed 674464 reads in 105.799 CPU sec, 3.520 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279881, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 343, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (356.78, 152.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674708 reads in 103.936 CPU sec, 3.469 real sec
[M::process] read 674754 sequences (100000207 bp) in 3.448 CPU sec, 3.879 real sec...
sorting block 17, #reads = 1076710 mem = 536879075 peak = 826552320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281451, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 342, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (354.82, 151.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674754 reads in 99.504 CPU sec, 3.304 real sec
[M::process] read 674762 sequences (100000111 bp) in 3.441 CPU sec, 3.696 real sec...
sorting block 18, #reads = 1077269 mem = 536881402 peak = 826552320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281032, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 341, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (354.63, 151.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674762 reads in 102.044 CPU sec, 3.349 real sec
[M::process] read 674902 sequences (100000032 bp) in 3.175 CPU sec, 3.383 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280544, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 341, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (354.28, 150.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 19, #reads = 1076936 mem = 536879841 peak = 826552320
[M::mem_process_seqs] Processed 674902 reads in 100.066 CPU sec, 3.221 real sec
[M::process] read 674440 sequences (100000171 bp) in 3.094 CPU sec, 3.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280934, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 348, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (359.90, 152.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674326 sequences (100000083 bp) in 2.789 CPU sec, 2.960 real sec...
[M::mem_process_seqs] Processed 674440 reads in 94.861 CPU sec, 3.047 real sec
sorting block 20, #reads = 1075843 mem = 536879148 peak = 826552320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280776, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 350, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (363.54, 155.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674326 reads in 89.312 CPU sec, 2.893 real sec
[M::process] read 674422 sequences (100000288 bp) in 3.369 CPU sec, 3.638 real sec...
sorting block 21, #reads = 1076277 mem = 536878751 peak = 826552320
[M::process] read 674372 sequences (100000034 bp) in 2.104 CPU sec, 2.212 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280976, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 351, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (364.53, 157.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674120 sequences (100000076 bp) in 1.710 CPU sec, 1.709 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280975, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 350, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (363.66, 157.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674422 reads in 157.144 CPU sec, 4.944 real sec
[M::mem_process_seqs] Processed 674372 reads in 105.623 CPU sec, 3.319 real sec
[M::process] read 675964 sequences (100000174 bp) in 2.203 CPU sec, 2.289 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281023, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 354, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (368.55, 159.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 248, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (314.45, 133.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RR
sorting block 22, #reads = 1075562 mem = 536879234 peak = 826552320
[M::process] read 678832 sequences (100000138 bp) in 2.507 CPU sec, 2.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281573, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 341, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (355.76, 156.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674120 reads in 149.423 CPU sec, 4.763 real sec
[M::mem_process_seqs] Processed 675964 reads in 97.783 CPU sec, 3.079 real sec
[M::process] read 678054 sequences (100000091 bp) in 2.454 CPU sec, 2.610 real sec...
sorting block 23, #reads = 1075136 mem = 536879339 peak = 826552320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281688, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 320, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (338.50, 154.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281909, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 325, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (342.33, 155.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677392 sequences (100000155 bp) in 2.709 CPU sec, 2.850 real sec...
[M::mem_process_seqs] Processed 678832 reads in 140.177 CPU sec, 4.458 real sec
[M::mem_process_seqs] Processed 678054 reads in 90.953 CPU sec, 2.863 real sec
sorting block 24, #reads = 1078425 mem = 536881371 peak = 826552320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280953, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (347.30, 156.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677228 sequences (100000211 bp) in 2.920 CPU sec, 3.093 real sec...
[M::mem_process_seqs] Processed 677392 reads in 81.375 CPU sec, 2.661 real sec
sorting block 25, #reads = 1081503 mem = 536878738 peak = 838733824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281201, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 332, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (347.67, 155.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677036 sequences (100000264 bp) in 2.669 CPU sec, 2.835 real sec...
[M::mem_process_seqs] Processed 677228 reads in 77.650 CPU sec, 2.510 real sec
sorting block 26, #reads = 1081192 mem = 536880343 peak = 838733824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281379, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 332, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (347.10, 153.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677036 reads in 75.863 CPU sec, 2.442 real sec
[M::process] read 676958 sequences (100000060 bp) in 2.435 CPU sec, 2.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281740, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 335, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (349.42, 154.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676958 reads in 74.749 CPU sec, 2.412 real sec
[M::process] read 676908 sequences (100000102 bp) in 2.674 CPU sec, 2.883 real sec...
sorting block 27, #reads = 1081266 mem = 536879436 peak = 838733824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281381, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (349.32, 153.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676908 reads in 73.147 CPU sec, 2.352 real sec
[M::process] read 676926 sequences (100000196 bp) in 2.437 CPU sec, 2.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281387, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 337, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (350.67, 155.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676926 reads in 71.424 CPU sec, 2.304 real sec
sorting block 28, #reads = 1080369 mem = 536877096 peak = 838733824
[M::process] read 676584 sequences (100000301 bp) in 2.353 CPU sec, 2.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281883, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 337, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (351.18, 154.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676584 reads in 72.358 CPU sec, 2.329 real sec
[M::process] read 676494 sequences (100000048 bp) in 2.476 CPU sec, 2.683 real sec...
sorting block 29, #reads = 1082047 mem = 536879507 peak = 838733824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281259, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 343, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (356.41, 157.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676086 sequences (100000183 bp) in 1.911 CPU sec, 1.973 real sec...
[M::mem_process_seqs] Processed 676494 reads in 72.114 CPU sec, 2.311 real sec
[M::process] read 676524 sequences (100000156 bp) in 2.002 CPU sec, 2.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281492, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 344, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (357.81, 158.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676012 sequences (100000116 bp) in 1.460 CPU sec, 1.453 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281592, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 343, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (356.55, 157.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676086 reads in 127.284 CPU sec, 3.995 real sec
[M::mem_process_seqs] Processed 676524 reads in 82.284 CPU sec, 2.581 real sec
sorting block 30, #reads = 1080490 mem = 536879137 peak = 838733824
[M::process] read 675762 sequences (100000229 bp) in 1.916 CPU sec, 1.984 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280750, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 342, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (355.17, 155.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1081258 mem = 536881997 peak = 931958784
[M::process] read 675878 sequences (100000221 bp) in 2.052 CPU sec, 2.130 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281002, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 343, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (355.97, 155.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676012 reads in 128.474 CPU sec, 4.069 real sec
[M::mem_process_seqs] Processed 675762 reads in 82.568 CPU sec, 2.594 real sec
[M::process] read 675586 sequences (100000223 bp) in 1.687 CPU sec, 1.710 real sec...
sorting block 32, #reads = 1079566 mem = 536878672 peak = 931958784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281387, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 344, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (356.64, 155.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675474 sequences (100000070 bp) in 2.000 CPU sec, 2.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281202, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (358.38, 155.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675878 reads in 125.374 CPU sec, 3.959 real sec
[M::process] read 675440 sequences (100000045 bp) in 1.500 CPU sec, 1.499 real sec...
[M::mem_process_seqs] Processed 675586 reads in 81.486 CPU sec, 2.568 real sec
sorting block 33, #reads = 1080599 mem = 536878229 peak = 931958784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281143, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (358.18, 155.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675422 sequences (100000190 bp) in 2.242 CPU sec, 2.306 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281027, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (360.49, 156.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675474 reads in 127.329 CPU sec, 4.021 real sec
[M::process] read 675240 sequences (100000214 bp) in 1.608 CPU sec, 1.611 real sec...
[M::mem_process_seqs] Processed 675440 reads in 82.677 CPU sec, 2.600 real sec
sorting block 34, #reads = 1078999 mem = 536877966 peak = 998629376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281581, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (360.96, 157.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675188 sequences (100000044 bp) in 2.003 CPU sec, 2.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280929, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (360.91, 156.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675374 sequences (100000136 bp) in 1.570 CPU sec, 1.562 real sec...
[M::mem_process_seqs] Processed 675422 reads in 132.803 CPU sec, 4.204 real sec
sorting block 35, #reads = 1079533 mem = 536880462 peak = 998629376
[M::mem_process_seqs] Processed 675240 reads in 85.048 CPU sec, 2.685 real sec
sorting block 36, #reads = 1078969 mem = 536877551 peak = 1006952448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281379, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (361.76, 157.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675188 sequences (100000264 bp) in 2.426 CPU sec, 2.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280980, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (361.85, 157.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675188 reads in 131.276 CPU sec, 4.144 real sec
[M::process] read 675034 sequences (100000058 bp) in 1.832 CPU sec, 1.843 real sec...
[M::mem_process_seqs] Processed 675374 reads in 83.901 CPU sec, 2.626 real sec
sorting block 37, #reads = 1078713 mem = 536878850 peak = 1006952448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281741, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (359.93, 155.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674766 sequences (100000290 bp) in 2.056 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281306, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (360.66, 155.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675188 reads in 129.245 CPU sec, 4.093 real sec
[M::process] read 674702 sequences (100000188 bp) in 1.765 CPU sec, 1.783 real sec...
[M::mem_process_seqs] Processed 675034 reads in 84.417 CPU sec, 2.643 real sec
sorting block 38, #reads = 1078512 mem = 536878706 peak = 1006952448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281355, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 350, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (361.86, 155.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674888 sequences (100000231 bp) in 2.150 CPU sec, 2.239 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280644, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (359.92, 155.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674766 reads in 130.243 CPU sec, 4.126 real sec
[M::mem_process_seqs] Processed 674702 reads in 83.793 CPU sec, 2.630 real sec
[M::process] read 674948 sequences (100000129 bp) in 2.120 CPU sec, 2.188 real sec...
sorting block 39, #reads = 1077935 mem = 536878197 peak = 1006952448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280204, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 346, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (359.58, 155.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675034 sequences (100000281 bp) in 2.185 CPU sec, 2.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281204, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 346, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (359.31, 155.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674888 reads in 131.136 CPU sec, 4.167 real sec
sorting block 40, #reads = 1078688 mem = 536876719 peak = 1006952448
[M::mem_process_seqs] Processed 674948 reads in 85.163 CPU sec, 2.675 real sec
[M::process] read 675014 sequences (100000024 bp) in 2.016 CPU sec, 2.065 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280984, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 345, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (358.75, 155.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1077785 mem = 536879985 peak = 1006952448
[M::process] read 674866 sequences (100000140 bp) in 2.127 CPU sec, 2.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281136, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 345, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (359.21, 156.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675034 reads in 129.779 CPU sec, 4.109 real sec
[M::mem_process_seqs] Processed 675014 reads in 84.669 CPU sec, 2.657 real sec
[M::process] read 675018 sequences (100000079 bp) in 1.920 CPU sec, 1.976 real sec...
sorting block 42, #reads = 1077632 mem = 536880072 peak = 1006952448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281265, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 343, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (357.26, 154.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674554 sequences (100000018 bp) in 2.095 CPU sec, 2.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281202, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 341, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (354.65, 152.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674866 reads in 126.817 CPU sec, 4.025 real sec
[M::mem_process_seqs] Processed 675018 reads in 83.376 CPU sec, 2.616 real sec
[M::process] read 674606 sequences (100000189 bp) in 1.870 CPU sec, 1.923 real sec...
sorting block 43, #reads = 1079017 mem = 536879521 peak = 1006952448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280579, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 345, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (359.03, 154.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674654 sequences (100000191 bp) in 2.245 CPU sec, 2.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281148, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (360.12, 155.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674554 reads in 125.660 CPU sec, 3.986 real sec
[M::mem_process_seqs] Processed 674606 reads in 81.636 CPU sec, 2.565 real sec
sorting block 44, #reads = 1078701 mem = 536878275 peak = 1006952448
[M::process] read 674486 sequences (100000283 bp) in 1.882 CPU sec, 1.933 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280744, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 347, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (361.07, 156.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674364 sequences (100000058 bp) in 2.164 CPU sec, 2.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281220, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (366.37, 159.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674654 reads in 125.317 CPU sec, 3.983 real sec
sorting block 45, #reads = 1077778 mem = 536876095 peak = 1022537728
[M::mem_process_seqs] Processed 674486 reads in 81.813 CPU sec, 2.577 real sec
[M::process] read 674194 sequences (100000171 bp) in 2.428 CPU sec, 2.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280680, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 353, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (367.36, 159.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 46, #reads = 1077904 mem = 536882631 peak = 1022537728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280996, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 353, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (367.44, 159.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676362 sequences (100000292 bp) in 2.453 CPU sec, 2.612 real sec...
[M::mem_process_seqs] Processed 674364 reads in 124.985 CPU sec, 3.989 real sec
[M::mem_process_seqs] Processed 674194 reads in 81.634 CPU sec, 2.572 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281734, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 336, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (352.22, 157.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1076662 mem = 536878319 peak = 1022537728
[M::process] read 678904 sequences (100000054 bp) in 2.475 CPU sec, 2.624 real sec...
[M::mem_process_seqs] Processed 676362 reads in 69.241 CPU sec, 2.278 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282409, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 318, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866)
[M::mem_pestat] mean and std.dev: (335.55, 152.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 48, #reads = 1078032 mem = 536881426 peak = 1022537728
[M::mem_process_seqs] Processed 678904 reads in 68.936 CPU sec, 2.249 real sec
[M::process] read 678594 sequences (100000062 bp) in 2.300 CPU sec, 2.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282559, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 321, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (338.93, 154.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677310 sequences (100000108 bp) in 2.007 CPU sec, 2.095 real sec...
[M::mem_process_seqs] Processed 678594 reads in 68.406 CPU sec, 2.197 real sec
sorting block 49, #reads = 1080718 mem = 536878889 peak = 1022537728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282367, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 331, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (347.12, 155.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677220 sequences (100000283 bp) in 2.006 CPU sec, 2.089 real sec...
[M::mem_process_seqs] Processed 677310 reads in 66.275 CPU sec, 2.127 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281976, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 332, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (347.81, 155.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676536 sequences (100000210 bp) in 1.943 CPU sec, 2.018 real sec...
[M::mem_process_seqs] Processed 677220 reads in 66.536 CPU sec, 2.138 real sec
sorting block 50, #reads = 1081981 mem = 536878077 peak = 1022537728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281739, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 337, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (351.50, 155.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676416 sequences (100000126 bp) in 1.944 CPU sec, 2.020 real sec...
[M::mem_process_seqs] Processed 676536 reads in 66.599 CPU sec, 2.136 real sec
sorting block 51, #reads = 1080401 mem = 536878917 peak = 1022537728
[M::process] read 676330 sequences (100000284 bp) in 1.900 CPU sec, 1.981 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281664, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 339, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (353.03, 155.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676298 sequences (100000037 bp) in 1.410 CPU sec, 1.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281391, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 340, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (354.77, 157.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676416 reads in 119.315 CPU sec, 3.743 real sec
[M::mem_process_seqs] Processed 676330 reads in 77.765 CPU sec, 2.434 real sec
[M::process] read 675912 sequences (100000027 bp) in 1.853 CPU sec, 1.914 real sec...
sorting block 52, #reads = 1080255 mem = 536879185 peak = 1022537728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282029, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 343, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (357.17, 158.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675724 sequences (100000171 bp) in 2.000 CPU sec, 2.072 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281386, 1, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 141, 187)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (49, 279)
[M::mem_pestat] mean and std.dev: (153.00, 18.83)
[M::mem_pestat] low and high boundaries for proper pairs: (3, 325)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (359.43, 158.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676298 reads in 119.393 CPU sec, 3.784 real sec
[M::mem_process_seqs] Processed 675912 reads in 77.820 CPU sec, 2.448 real sec
[M::process] read 675848 sequences (100000080 bp) in 1.708 CPU sec, 1.738 real sec...
sorting block 53, #reads = 1080624 mem = 536879403 peak = 1022537728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281558, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (359.85, 158.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675742 sequences (100000188 bp) in 1.825 CPU sec, 1.889 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281754, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (362.58, 160.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675724 reads in 118.524 CPU sec, 3.749 real sec
[M::process] read 675498 sequences (100000059 bp) in 1.468 CPU sec, 1.477 real sec...
[M::mem_process_seqs] Processed 675848 reads in 76.410 CPU sec, 2.399 real sec
sorting block 54, #reads = 1080758 mem = 536881027 peak = 1022537728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281226, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (363.29, 161.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675456 sequences (100000040 bp) in 2.123 CPU sec, 2.193 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281774, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 351, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (364.69, 161.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675742 reads in 118.889 CPU sec, 3.762 real sec
sorting block 55, #reads = 1080089 mem = 536880378 peak = 1056944128
[M::mem_process_seqs] Processed 675498 reads in 77.132 CPU sec, 2.419 real sec
[M::process] read 675182 sequences (100000071 bp) in 1.665 CPU sec, 1.696 real sec...
sorting block 56, #reads = 1079022 mem = 536880365 peak = 1058660352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281141, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (364.10, 160.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674942 sequences (100000041 bp) in 1.995 CPU sec, 2.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281161, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (365.44, 160.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675456 reads in 118.869 CPU sec, 3.770 real sec
[M::mem_process_seqs] Processed 675182 reads in 77.648 CPU sec, 2.433 real sec
[M::process] read 675604 sequences (100000178 bp) in 1.752 CPU sec, 1.789 real sec...
sorting block 57, #reads = 1079713 mem = 536880222 peak = 1058660352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281327, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (365.89, 160.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675906 sequences (100000267 bp) in 2.174 CPU sec, 2.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281376, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (361.68, 161.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674942 reads in 121.817 CPU sec, 3.856 real sec
[M::mem_process_seqs] Processed 675604 reads in 79.068 CPU sec, 2.480 real sec
[M::process] read 675132 sequences (100000087 bp) in 1.774 CPU sec, 1.807 real sec...
sorting block 58, #reads = 1079587 mem = 536881520 peak = 1058660352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281542, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (360.49, 161.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674850 sequences (100000272 bp) in 1.924 CPU sec, 2.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280935, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (366.03, 161.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675906 reads in 120.659 CPU sec, 3.829 real sec
[M::mem_process_seqs] Processed 675132 reads in 77.923 CPU sec, 2.445 real sec
sorting block 59, #reads = 1079597 mem = 536878634 peak = 1058660352
[M::process] read 674768 sequences (100000080 bp) in 1.783 CPU sec, 1.816 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281236, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (366.75, 160.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674620 sequences (100000019 bp) in 2.123 CPU sec, 2.203 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281300, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (363.71, 158.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674850 reads in 119.839 CPU sec, 3.794 real sec
sorting block 60, #reads = 1079379 mem = 536887498 peak = 1058660352
[M::mem_process_seqs] Processed 674768 reads in 76.889 CPU sec, 2.412 real sec
[M::process] read 674394 sequences (100000250 bp) in 1.862 CPU sec, 1.900 real sec...
sorting block 61, #reads = 1079674 mem = 536879497 peak = 1068273664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280675, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 351, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (364.42, 158.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674414 sequences (100000297 bp) in 1.931 CPU sec, 2.010 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280654, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (367.04, 160.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674620 reads in 121.213 CPU sec, 3.846 real sec
[M::mem_process_seqs] Processed 674394 reads in 79.886 CPU sec, 2.507 real sec
[M::process] read 674686 sequences (100000259 bp) in 1.815 CPU sec, 1.851 real sec...
sorting block 62, #reads = 1078018 mem = 536878854 peak = 1068273664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280577, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 350, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (365.31, 158.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674714 sequences (100000130 bp) in 2.153 CPU sec, 2.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280968, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 348, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (362.78, 158.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674414 reads in 120.612 CPU sec, 3.818 real sec
[M::process] read 674800 sequences (100000073 bp) in 1.808 CPU sec, 1.854 real sec...
sorting block 63, #reads = 1078533 mem = 536882018 peak = 1068273664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281237, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (362.95, 158.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674714 reads in 67.594 CPU sec, 2.131 real sec
[M::process] read 675006 sequences (100000075 bp) in 1.533 CPU sec, 1.541 real sec...
[M::mem_process_seqs] Processed 674686 reads in 163.019 CPU sec, 5.133 real sec
sorting block 64, #reads = 1079339 mem = 536882078 peak = 1068273664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281406, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (363.92, 159.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675078 sequences (100000012 bp) in 2.299 CPU sec, 2.379 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281397, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 345, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (359.67, 157.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674800 reads in 133.317 CPU sec, 4.213 real sec
[M::mem_process_seqs] Processed 675006 reads in 79.842 CPU sec, 2.512 real sec
[M::process] read 675184 sequences (100000010 bp) in 1.988 CPU sec, 2.075 real sec...
sorting block 65, #reads = 1079329 mem = 536879733 peak = 1068273664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281568, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (362.27, 159.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674764 sequences (100000007 bp) in 2.024 CPU sec, 2.100 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281079, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 344, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (360.51, 159.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675078 reads in 122.853 CPU sec, 3.894 real sec
sorting block 66, #reads = 1078472 mem = 536878759 peak = 1085202432
[M::process] read 674868 sequences (100000227 bp) in 2.058 CPU sec, 2.123 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281303, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (363.50, 159.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674764 reads in 67.707 CPU sec, 2.127 real sec
[M::process] read 674414 sequences (100000046 bp) in 2.073 CPU sec, 2.141 real sec...
[M::mem_process_seqs] Processed 675184 reads in 180.416 CPU sec, 5.677 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281789, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 347, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (361.61, 158.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 67, #reads = 1078373 mem = 536878466 peak = 1085202432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280907, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 349, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (363.28, 157.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674868 reads in 133.761 CPU sec, 4.237 real sec
[M::mem_process_seqs] Processed 674414 reads in 79.701 CPU sec, 2.524 real sec
[M::process] read 674362 sequences (100000028 bp) in 3.161 CPU sec, 3.414 real sec...
sorting block 68, #reads = 1077521 mem = 536877166 peak = 1085202432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281475, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 348, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (362.38, 157.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674362 reads in 68.575 CPU sec, 2.257 real sec
[M::process] read 674188 sequences (100000195 bp) in 2.392 CPU sec, 2.563 real sec...
sorting block 69, #reads = 1076502 mem = 536878554 peak = 1088958464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280984, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 354, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (367.68, 160.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674334 sequences (100000237 bp) in 1.931 CPU sec, 2.016 real sec...
[M::mem_process_seqs] Processed 674188 reads in 67.562 CPU sec, 2.175 real sec
sorting block 70, #reads = 1076314 mem = 536880404 peak = 1088958464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281113, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 353, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (367.20, 159.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674160 sequences (100000175 bp) in 1.909 CPU sec, 1.986 real sec...
[M::mem_process_seqs] Processed 674334 reads in 68.015 CPU sec, 2.180 real sec
[M::process] read 674540 sequences (100000080 bp) in 1.913 CPU sec, 1.996 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281537, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 356, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (370.67, 161.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678418 sequences (100000030 bp) in 1.510 CPU sec, 1.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281645, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 354, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (369.07, 162.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674160 reads in 122.704 CPU sec, 3.850 real sec
[M::mem_process_seqs] Processed 674540 reads in 81.646 CPU sec, 2.557 real sec
sorting block 71, #reads = 1077124 mem = 536877339 peak = 1088958464
[M::process] read 679078 sequences (100000183 bp) in 1.848 CPU sec, 1.920 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282701, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 323, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (342.11, 157.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678170 sequences (100000223 bp) in 2.023 CPU sec, 2.103 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282329, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 317, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (337.12, 156.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678418 reads in 123.031 CPU sec, 3.900 real sec
sorting block 72, #reads = 1075363 mem = 536880235 peak = 1088958464
[M::mem_process_seqs] Processed 679078 reads in 78.925 CPU sec, 2.481 real sec
[M::process] read 676698 sequences (100000052 bp) in 1.653 CPU sec, 1.678 real sec...
sorting block 73, #reads = 1081466 mem = 536881858 peak = 1109856256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282236, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 326, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (345.06, 159.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676530 sequences (100000094 bp) in 2.014 CPU sec, 2.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281471, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 338, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (354.94, 160.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678170 reads in 118.693 CPU sec, 3.751 real sec
[M::mem_process_seqs] Processed 676698 reads in 77.183 CPU sec, 2.421 real sec
[M::process] read 676640 sequences (100000058 bp) in 1.664 CPU sec, 1.689 real sec...
sorting block 74, #reads = 1081952 mem = 536878288 peak = 1109856256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281480, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 340, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (356.91, 160.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676098 sequences (100000213 bp) in 2.117 CPU sec, 2.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281685, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 342, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (357.74, 161.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676530 reads in 117.263 CPU sec, 3.714 real sec
[M::mem_process_seqs] Processed 676640 reads in 76.449 CPU sec, 2.399 real sec
[M::process] read 676068 sequences (100000021 bp) in 1.704 CPU sec, 1.723 real sec...
sorting block 75, #reads = 1080762 mem = 536884550 peak = 1109856256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281098, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 343, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (359.19, 160.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675878 sequences (100000101 bp) in 2.069 CPU sec, 2.137 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281318, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 345, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (360.19, 161.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676098 reads in 117.071 CPU sec, 3.708 real sec
[M::mem_process_seqs] Processed 676068 reads in 76.167 CPU sec, 2.389 real sec
sorting block 76, #reads = 1080522 mem = 536880354 peak = 1109856256
[M::process] read 675710 sequences (100000029 bp) in 1.905 CPU sec, 1.941 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281134, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (361.34, 161.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675386 sequences (100000136 bp) in 2.174 CPU sec, 2.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282005, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (363.91, 162.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675878 reads in 117.029 CPU sec, 3.706 real sec
sorting block 77, #reads = 1080832 mem = 536878744 peak = 1109856256
[M::mem_process_seqs] Processed 675710 reads in 75.801 CPU sec, 2.381 real sec
[M::process] read 675288 sequences (100000160 bp) in 2.162 CPU sec, 2.230 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281685, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 351, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (366.34, 162.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 78, #reads = 1080264 mem = 536878162 peak = 1109856256
[M::process] read 675396 sequences (100000259 bp) in 1.938 CPU sec, 1.996 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281075, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (367.04, 163.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675386 reads in 117.699 CPU sec, 3.733 real sec
[M::mem_process_seqs] Processed 675288 reads in 77.292 CPU sec, 2.426 real sec
[M::process] read 675252 sequences (100000127 bp) in 1.931 CPU sec, 1.999 real sec...
sorting block 79, #reads = 1080400 mem = 536878425 peak = 1109856256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281344, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (366.64, 162.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674854 sequences (100000027 bp) in 1.992 CPU sec, 2.061 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281479, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 353, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (368.12, 163.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675396 reads in 117.871 CPU sec, 3.737 real sec
[M::mem_process_seqs] Processed 675252 reads in 76.676 CPU sec, 2.411 real sec
sorting block 80, #reads = 1079925 mem = 536879286 peak = 1109856256
[M::process] read 674830 sequences (100000241 bp) in 1.897 CPU sec, 1.962 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280998, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 354, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (369.13, 163.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674888 sequences (100000125 bp) in 2.010 CPU sec, 2.080 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281494, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 354, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (368.15, 161.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674854 reads in 117.272 CPU sec, 3.716 real sec
[M::mem_process_seqs] Processed 674830 reads in 76.669 CPU sec, 2.408 real sec
sorting block 81, #reads = 1080127 mem = 536882514 peak = 1109856256
[M::process] read 675884 sequences (100000150 bp) in 2.336 CPU sec, 2.453 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280845, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 353, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (366.85, 160.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675172 sequences (100000065 bp) in 2.062 CPU sec, 2.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281520, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (362.03, 161.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674888 reads in 119.257 CPU sec, 3.804 real sec
sorting block 82, #reads = 1079470 mem = 536879089 peak = 1109856256
[M::mem_process_seqs] Processed 675884 reads in 77.822 CPU sec, 2.451 real sec
[M::process] read 674634 sequences (100000028 bp) in 2.093 CPU sec, 2.187 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281892, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (365.03, 161.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 83, #reads = 1079303 mem = 536881276 peak = 1112002560
[M::process] read 674498 sequences (100000171 bp) in 1.882 CPU sec, 1.925 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281304, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 355, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (369.89, 162.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675172 reads in 120.053 CPU sec, 3.808 real sec
[M::mem_process_seqs] Processed 674634 reads in 77.980 CPU sec, 2.452 real sec
sorting block 84, #reads = 1080048 mem = 536875449 peak = 1112002560
[M::process] read 674612 sequences (100000240 bp) in 2.064 CPU sec, 2.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280660, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 356, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (370.40, 161.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674534 sequences (100000076 bp) in 1.928 CPU sec, 1.979 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281030, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (366.18, 159.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674498 reads in 120.431 CPU sec, 3.821 real sec
[M::mem_process_seqs] Processed 674612 reads in 79.284 CPU sec, 2.494 real sec
sorting block 85, #reads = 1078590 mem = 536880599 peak = 1112002560
[M::process] read 674596 sequences (100000206 bp) in 2.048 CPU sec, 2.132 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281206, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 352, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (366.01, 158.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674372 sequences (100000192 bp) in 1.927 CPU sec, 1.979 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281279, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 348, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (361.71, 155.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674534 reads in 119.870 CPU sec, 3.800 real sec
[M::mem_process_seqs] Processed 674596 reads in 77.810 CPU sec, 2.448 real sec
sorting block 86, #reads = 1078729 mem = 536877334 peak = 1112002560
[M::process] read 674780 sequences (100000082 bp) in 2.032 CPU sec, 2.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281005, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 348, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (361.55, 155.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 87, #reads = 1079359 mem = 536879469 peak = 1112002560
[M::process] read 674830 sequences (100000007 bp) in 1.960 CPU sec, 2.023 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280921, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 345, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (358.57, 154.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674372 reads in 119.828 CPU sec, 3.801 real sec
[M::mem_process_seqs] Processed 674780 reads in 78.558 CPU sec, 2.469 real sec
[M::process] read 674624 sequences (100000032 bp) in 1.954 CPU sec, 2.014 real sec...
sorting block 88, #reads = 1078059 mem = 536881153 peak = 1112002560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280946, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 343, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (357.91, 155.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674810 sequences (100000143 bp) in 1.995 CPU sec, 2.060 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281536, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 347, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (360.58, 155.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674830 reads in 118.969 CPU sec, 3.771 real sec
[M::mem_process_seqs] Processed 674624 reads in 77.890 CPU sec, 2.443 real sec
[M::process] read 674784 sequences (100000249 bp) in 1.865 CPU sec, 1.916 real sec...
sorting block 89, #reads = 1078953 mem = 536880796 peak = 1112002560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280903, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 347, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (361.30, 157.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674654 sequences (100000288 bp) in 2.435 CPU sec, 2.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280914, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 344, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (359.36, 156.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674810 reads in 120.060 CPU sec, 3.811 real sec
[M::mem_process_seqs] Processed 674784 reads in 78.244 CPU sec, 2.468 real sec
sorting block 90, #reads = 1078886 mem = 536884532 peak = 1112002560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281797, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 347, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (360.76, 156.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674698 sequences (100000272 bp) in 2.652 CPU sec, 2.834 real sec...
[M::mem_process_seqs] Processed 674654 reads in 66.742 CPU sec, 2.196 real sec
sorting block 91, #reads = 1077779 mem = 536877939 peak = 1112002560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281387, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 348, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (362.43, 157.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674698 reads in 66.485 CPU sec, 2.173 real sec
[M::process] read 674394 sequences (100000248 bp) in 2.966 CPU sec, 3.245 real sec...
sorting block 92, #reads = 1078215 mem = 536882344 peak = 1112002560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281444, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 348, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (362.22, 156.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674394 reads in 65.976 CPU sec, 2.130 real sec
[M::process] read 674322 sequences (100000134 bp) in 2.180 CPU sec, 2.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281091, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 350, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (364.16, 157.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674322 reads in 66.326 CPU sec, 2.138 real sec
[M::process] read 674216 sequences (100000244 bp) in 2.062 CPU sec, 2.149 real sec...
sorting block 93, #reads = 1078818 mem = 536878295 peak = 1112002560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281373, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 351, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (366.60, 159.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674352 sequences (100000254 bp) in 2.002 CPU sec, 2.078 real sec...
[M::mem_process_seqs] Processed 674216 reads in 66.863 CPU sec, 2.153 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281207, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 354, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (367.84, 159.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674146 sequences (100000003 bp) in 1.972 CPU sec, 2.056 real sec...
[M::mem_process_seqs] Processed 674352 reads in 67.118 CPU sec, 2.161 real sec
sorting block 94, #reads = 1078141 mem = 536879509 peak = 1112002560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281671, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 354, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (368.63, 160.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674256 sequences (100000239 bp) in 1.894 CPU sec, 1.973 real sec...
[M::mem_process_seqs] Processed 674146 reads in 65.918 CPU sec, 2.114 real sec
sorting block 95, #reads = 1078068 mem = 536879162 peak = 1112002560
[M::process] read 673978 sequences (100000097 bp) in 1.858 CPU sec, 1.917 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281651, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 355, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (368.87, 160.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679866 sequences (100000280 bp) in 1.470 CPU sec, 1.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280866, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 356, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (370.21, 160.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674256 reads in 120.438 CPU sec, 3.778 real sec
[M::mem_process_seqs] Processed 673978 reads in 79.407 CPU sec, 2.491 real sec
[M::process] read 680482 sequences (100000229 bp) in 1.945 CPU sec, 1.997 real sec...
sorting block 96, #reads = 1077181 mem = 536881204 peak = 1112002560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282674, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 315, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (335.27, 156.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679344 sequences (100000158 bp) in 2.080 CPU sec, 2.141 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282953, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 309, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (330.92, 154.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679866 reads in 139.636 CPU sec, 4.416 real sec
[M::process] read 679432 sequences (100000263 bp) in 1.544 CPU sec, 1.550 real sec...
[M::mem_process_seqs] Processed 680482 reads in 90.892 CPU sec, 2.855 real sec
sorting block 97, #reads = 1074744 mem = 536891063 peak = 1112002560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282744, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 319, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (339.64, 157.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679554 sequences (100000103 bp) in 2.210 CPU sec, 2.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282771, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 323, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (341.23, 157.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679006 sequences (100000020 bp) in 1.670 CPU sec, 1.668 real sec...
[M::mem_process_seqs] Processed 679344 reads in 136.252 CPU sec, 4.298 real sec
sorting block 98, #reads = 1077372 mem = 536877872 peak = 1112002560
[M::mem_process_seqs] Processed 679432 reads in 86.138 CPU sec, 2.714 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282825, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 320, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (336.92, 153.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678836 sequences (100000225 bp) in 1.948 CPU sec, 1.988 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282478, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 324, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (340.82, 154.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677942 sequences (100000246 bp) in 1.590 CPU sec, 1.599 real sec...
[M::mem_process_seqs] Processed 679554 reads in 131.012 CPU sec, 4.144 real sec
sorting block 99, #reads = 1077529 mem = 536878513 peak = 1202409472
[M::mem_process_seqs] Processed 679006 reads in 84.021 CPU sec, 2.641 real sec
sorting block 100, #reads = 1077830 mem = 536876762 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282338, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (341.35, 154.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677716 sequences (100000283 bp) in 2.295 CPU sec, 2.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282178, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (347.85, 155.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678836 reads in 130.549 CPU sec, 4.119 real sec
[M::process] read 677636 sequences (100000063 bp) in 1.772 CPU sec, 1.776 real sec...
[M::mem_process_seqs] Processed 677942 reads in 84.246 CPU sec, 2.645 real sec
sorting block 101, #reads = 1078595 mem = 536878863 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282496, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (349.87, 157.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677314 sequences (100000055 bp) in 2.721 CPU sec, 2.864 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282325, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (349.55, 156.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677716 reads in 130.360 CPU sec, 4.134 real sec
sorting block 102, #reads = 1079157 mem = 536880200 peak = 1204248576
[M::process] read 677040 sequences (100000040 bp) in 2.523 CPU sec, 2.661 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282046, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 338, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (351.76, 156.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677314 reads in 73.642 CPU sec, 2.322 real sec
[M::mem_process_seqs] Processed 677636 reads in 207.590 CPU sec, 6.538 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282211, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (355.16, 159.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 777, 777)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1983)
[M::mem_pestat] mean and std.dev: (587.40, 289.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2586)
[M::mem_pestat] skip orientation RR
[M::process] read 677046 sequences (100000086 bp) in 2.597 CPU sec, 2.752 real sec...
[M::mem_process_seqs] Processed 677040 reads in 84.117 CPU sec, 2.703 real sec
sorting block 103, #reads = 1077765 mem = 536878979 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281751, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 340, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (353.52, 157.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677046 reads in 73.403 CPU sec, 2.393 real sec
[M::process] read 677088 sequences (100000276 bp) in 2.678 CPU sec, 2.853 real sec...
sorting block 104, #reads = 1078578 mem = 536880518 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282522, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 339, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (352.42, 156.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677088 reads in 71.707 CPU sec, 2.322 real sec
[M::process] read 677076 sequences (100000188 bp) in 2.308 CPU sec, 2.467 real sec...
sorting block 105, #reads = 1078008 mem = 536881261 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281818, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 340, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (354.01, 157.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676850 sequences (100000035 bp) in 1.987 CPU sec, 2.065 real sec...
[M::mem_process_seqs] Processed 677076 reads in 71.771 CPU sec, 2.307 real sec
[M::process] read 676716 sequences (100000296 bp) in 1.893 CPU sec, 1.968 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282103, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 339, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (352.90, 155.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676828 sequences (100000000 bp) in 1.506 CPU sec, 1.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281598, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 342, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (355.19, 156.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676850 reads in 130.287 CPU sec, 4.086 real sec
sorting block 106, #reads = 1078886 mem = 536878362 peak = 1204248576
[M::mem_process_seqs] Processed 676716 reads in 84.185 CPU sec, 2.639 real sec
[M::process] read 676670 sequences (100000062 bp) in 1.891 CPU sec, 1.951 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281672, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 341, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (353.73, 156.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 107, #reads = 1077115 mem = 536878270 peak = 1204248576
[M::process] read 676782 sequences (100000188 bp) in 2.086 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281404, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 343, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (355.63, 156.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676828 reads in 133.719 CPU sec, 4.232 real sec
[M::process] read 676554 sequences (100000033 bp) in 1.544 CPU sec, 1.558 real sec...
[M::mem_process_seqs] Processed 676670 reads in 86.575 CPU sec, 2.721 real sec
sorting block 108, #reads = 1076492 mem = 536879049 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281735, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 342, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (354.53, 156.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676600 sequences (100000121 bp) in 2.132 CPU sec, 2.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281714, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 342, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (355.74, 157.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676304 sequences (100000138 bp) in 1.510 CPU sec, 1.511 real sec...
[M::mem_process_seqs] Processed 676782 reads in 132.049 CPU sec, 4.170 real sec
[M::mem_process_seqs] Processed 676554 reads in 84.339 CPU sec, 2.661 real sec
sorting block 109, #reads = 1075988 mem = 536879510 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281970, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 343, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (356.67, 158.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675922 sequences (100000254 bp) in 2.175 CPU sec, 2.234 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281576, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (357.95, 157.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675972 sequences (100000110 bp) in 1.570 CPU sec, 1.572 real sec...
[M::mem_process_seqs] Processed 676600 reads in 129.475 CPU sec, 4.086 real sec
sorting block 110, #reads = 1076440 mem = 536877377 peak = 1204248576
[M::mem_process_seqs] Processed 676304 reads in 83.285 CPU sec, 2.621 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281523, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 344, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (357.04, 156.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675724 sequences (100000030 bp) in 2.217 CPU sec, 2.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281281, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (357.33, 155.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675868 sequences (100000162 bp) in 1.580 CPU sec, 1.576 real sec...
[M::mem_process_seqs] Processed 675922 reads in 132.594 CPU sec, 4.187 real sec
sorting block 111, #reads = 1077530 mem = 536879561 peak = 1204248576
[M::mem_process_seqs] Processed 675972 reads in 85.004 CPU sec, 2.682 real sec
sorting block 112, #reads = 1076535 mem = 536878674 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281828, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (357.34, 154.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675596 sequences (100000205 bp) in 2.242 CPU sec, 2.316 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281487, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 343, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (354.96, 153.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675458 sequences (100000301 bp) in 1.610 CPU sec, 1.605 real sec...
[M::mem_process_seqs] Processed 675724 reads in 134.435 CPU sec, 4.243 real sec
[M::mem_process_seqs] Processed 675868 reads in 86.432 CPU sec, 2.723 real sec
sorting block 113, #reads = 1075724 mem = 536883090 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281675, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 343, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (354.99, 152.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674830 sequences (100000199 bp) in 2.333 CPU sec, 2.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281466, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 343, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (354.96, 152.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675278 sequences (100000064 bp) in 1.600 CPU sec, 1.601 real sec...
[M::mem_process_seqs] Processed 675596 reads in 132.201 CPU sec, 4.168 real sec
[M::mem_process_seqs] Processed 675458 reads in 84.174 CPU sec, 2.650 real sec
sorting block 114, #reads = 1073726 mem = 536878822 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281012, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (360.35, 154.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674482 sequences (100000023 bp) in 2.267 CPU sec, 2.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281787, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 346, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (357.90, 153.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675014 sequences (100000105 bp) in 1.620 CPU sec, 1.625 real sec...
[M::mem_process_seqs] Processed 674830 reads in 131.547 CPU sec, 4.152 real sec
sorting block 115, #reads = 1075964 mem = 536881656 peak = 1204248576
[M::mem_process_seqs] Processed 675278 reads in 84.611 CPU sec, 2.661 real sec
sorting block 116, #reads = 1074231 mem = 536881475 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281250, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 351, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (362.86, 154.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674714 sequences (100000052 bp) in 2.322 CPU sec, 2.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281403, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 346, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (358.87, 154.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674482 reads in 131.442 CPU sec, 4.145 real sec
[M::process] read 674950 sequences (100000045 bp) in 1.650 CPU sec, 1.648 real sec...
[M::mem_process_seqs] Processed 675014 reads in 85.164 CPU sec, 2.675 real sec
sorting block 117, #reads = 1074560 mem = 536879378 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281384, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (364.34, 157.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675066 sequences (100000199 bp) in 2.374 CPU sec, 2.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281589, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (362.45, 157.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674714 reads in 133.096 CPU sec, 4.201 real sec
[M::process] read 684492 sequences (100000238 bp) in 1.785 CPU sec, 1.785 real sec...
[M::mem_process_seqs] Processed 674950 reads in 86.598 CPU sec, 2.719 real sec
sorting block 118, #reads = 1074622 mem = 536879342 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281701, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (363.08, 158.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684324 sequences (100000188 bp) in 2.372 CPU sec, 2.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284520, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 283, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (303.76, 140.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683286 sequences (100000018 bp) in 1.700 CPU sec, 1.706 real sec...
[M::mem_process_seqs] Processed 675066 reads in 143.122 CPU sec, 4.520 real sec
[M::mem_process_seqs] Processed 684492 reads in 98.831 CPU sec, 3.111 real sec
sorting block 119, #reads = 1073465 mem = 536879553 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284759, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 287, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (305.36, 139.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682818 sequences (100000241 bp) in 2.236 CPU sec, 2.301 real sec...
[M::process] read 681976 sequences (100000230 bp) in 1.700 CPU sec, 1.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284104, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 295, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (313.77, 144.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1044)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684324 reads in 154.006 CPU sec, 4.873 real sec
sorting block 120, #reads = 1069865 mem = 536879770 peak = 1204248576
[M::mem_process_seqs] Processed 683286 reads in 98.501 CPU sec, 3.120 real sec
sorting block 121, #reads = 1068815 mem = 536879591 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284472, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 296, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831)
[M::mem_pestat] mean and std.dev: (314.51, 143.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1041)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681338 sequences (100000008 bp) in 2.417 CPU sec, 2.486 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284431, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 303, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (320.66, 146.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680148 sequences (100000179 bp) in 1.740 CPU sec, 1.740 real sec...
[M::mem_process_seqs] Processed 682818 reads in 147.620 CPU sec, 4.662 real sec
[M::mem_process_seqs] Processed 681976 reads in 94.740 CPU sec, 2.989 real sec
sorting block 122, #reads = 1071846 mem = 536877936 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283270, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 307, 422)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (322.91, 146.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1067)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680098 sequences (100000224 bp) in 2.541 CPU sec, 2.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283005, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858)
[M::mem_pestat] mean and std.dev: (327.87, 145.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679418 sequences (100000042 bp) in 1.690 CPU sec, 1.689 real sec...
[M::mem_process_seqs] Processed 681338 reads in 144.962 CPU sec, 4.576 real sec
[M::mem_process_seqs] Processed 680148 reads in 94.221 CPU sec, 2.969 real sec
sorting block 123, #reads = 1071617 mem = 536879126 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283143, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (328.21, 146.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1077)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679140 sequences (100000199 bp) in 2.404 CPU sec, 2.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283274, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 318, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (333.28, 148.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 220, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (172, 292)
[M::mem_pestat] mean and std.dev: (227.00, 12.33)
[M::mem_pestat] low and high boundaries for proper pairs: (148, 316)
[M::mem_pestat] skip orientation RR
[M::process] read 678842 sequences (100000276 bp) in 1.840 CPU sec, 1.834 real sec...
[M::mem_process_seqs] Processed 680098 reads in 152.037 CPU sec, 4.802 real sec
sorting block 124, #reads = 1071795 mem = 536878496 peak = 1204248576
[M::mem_process_seqs] Processed 679418 reads in 105.117 CPU sec, 3.322 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282762, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 320, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (334.56, 148.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1090)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678450 sequences (100000072 bp) in 2.947 CPU sec, 3.024 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283055, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 323, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871)
[M::mem_pestat] mean and std.dev: (335.95, 148.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679140 reads in 165.221 CPU sec, 5.204 real sec
sorting block 125, #reads = 1072439 mem = 536880402 peak = 1204248576
[M::process] read 678082 sequences (100000199 bp) in 2.492 CPU sec, 2.501 real sec...
[M::mem_process_seqs] Processed 678842 reads in 105.203 CPU sec, 3.296 real sec
sorting block 126, #reads = 1072729 mem = 536876941 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283295, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 325, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (338.73, 149.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677884 sequences (100000029 bp) in 2.909 CPU sec, 2.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282693, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (341.93, 151.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678450 reads in 152.031 CPU sec, 4.808 real sec
[M::mem_process_seqs] Processed 678082 reads in 98.477 CPU sec, 3.091 real sec
[M::process] read 677802 sequences (100000198 bp) in 2.517 CPU sec, 2.563 real sec...
sorting block 127, #reads = 1073517 mem = 536881198 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282949, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (343.18, 151.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677774 sequences (100000237 bp) in 2.684 CPU sec, 2.811 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282859, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (343.76, 151.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677884 reads in 152.040 CPU sec, 4.837 real sec
[M::mem_process_seqs] Processed 677802 reads in 99.172 CPU sec, 3.122 real sec
sorting block 128, #reads = 1074359 mem = 536879043 peak = 1204248576
[M::process] read 677868 sequences (100000165 bp) in 2.399 CPU sec, 2.465 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282661, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (343.01, 151.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677468 sequences (100000009 bp) in 2.376 CPU sec, 2.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282864, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (342.73, 151.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677774 reads in 150.363 CPU sec, 4.765 real sec
[M::mem_process_seqs] Processed 677868 reads in 99.134 CPU sec, 3.115 real sec
sorting block 129, #reads = 1074660 mem = 536879157 peak = 1204248576
[M::process] read 676896 sequences (100000201 bp) in 2.035 CPU sec, 2.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282857, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (347.31, 154.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676552 sequences (100000258 bp) in 2.548 CPU sec, 2.609 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282137, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 339, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (352.92, 156.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676150 sequences (100000115 bp) in 1.610 CPU sec, 1.606 real sec...
[M::mem_process_seqs] Processed 677468 reads in 149.216 CPU sec, 4.718 real sec
sorting block 130, #reads = 1073816 mem = 536877455 peak = 1204248576
[M::mem_process_seqs] Processed 676896 reads in 95.857 CPU sec, 3.016 real sec
sorting block 131, #reads = 1074714 mem = 536879804 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282038, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 344, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (357.95, 159.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676074 sequences (100000090 bp) in 2.460 CPU sec, 2.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282047, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 346, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (361.27, 161.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675906 sequences (100000016 bp) in 1.690 CPU sec, 1.686 real sec...
[M::mem_process_seqs] Processed 676552 reads in 147.355 CPU sec, 4.653 real sec
[M::mem_process_seqs] Processed 676150 reads in 95.479 CPU sec, 3.011 real sec
sorting block 132, #reads = 1075069 mem = 536879414 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281747, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 346, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (361.94, 161.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675540 sequences (100000096 bp) in 2.308 CPU sec, 2.359 real sec...
[M::process] read 675600 sequences (100000103 bp) in 1.690 CPU sec, 1.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281995, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 345, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (360.37, 159.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676074 reads in 146.528 CPU sec, 4.625 real sec
[M::mem_process_seqs] Processed 675906 reads in 95.544 CPU sec, 3.021 real sec
sorting block 133, #reads = 1074421 mem = 536878979 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280826, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (361.02, 159.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675366 sequences (100000037 bp) in 2.345 CPU sec, 2.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281924, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (362.34, 160.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675258 sequences (100000232 bp) in 1.700 CPU sec, 1.702 real sec...
[M::mem_process_seqs] Processed 675540 reads in 145.450 CPU sec, 4.591 real sec
sorting block 134, #reads = 1074976 mem = 536877979 peak = 1204248576
[M::mem_process_seqs] Processed 675600 reads in 93.966 CPU sec, 2.971 real sec
sorting block 135, #reads = 1075085 mem = 536882935 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281359, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (363.73, 161.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675046 sequences (100000065 bp) in 2.379 CPU sec, 2.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281439, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (365.41, 162.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675088 sequences (100000004 bp) in 1.690 CPU sec, 1.687 real sec...
[M::mem_process_seqs] Processed 675366 reads in 144.078 CPU sec, 4.547 real sec
[M::mem_process_seqs] Processed 675258 reads in 92.486 CPU sec, 2.924 real sec
sorting block 136, #reads = 1074519 mem = 536879249 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281252, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 352, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (367.50, 163.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674820 sequences (100000092 bp) in 2.271 CPU sec, 2.319 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281459, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 352, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (367.98, 164.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674764 sequences (100000000 bp) in 1.700 CPU sec, 1.699 real sec...
[M::mem_process_seqs] Processed 675046 reads in 140.007 CPU sec, 4.418 real sec
[M::mem_process_seqs] Processed 675088 reads in 89.904 CPU sec, 2.838 real sec
sorting block 137, #reads = 1074662 mem = 536878837 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281199, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 355, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (371.13, 165.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677176 sequences (100000136 bp) in 2.881 CPU sec, 2.924 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281168, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 356, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (372.39, 166.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674820 reads in 147.649 CPU sec, 4.659 real sec
[M::mem_process_seqs] Processed 674764 reads in 99.183 CPU sec, 3.104 real sec
[M::process] read 680678 sequences (100000237 bp) in 2.093 CPU sec, 2.105 real sec...
sorting block 138, #reads = 1074456 mem = 536883068 peak = 1204248576
[M::process] read 679642 sequences (100000053 bp) in 2.229 CPU sec, 2.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281578, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (351.82, 160.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678926 sequences (100000140 bp) in 1.650 CPU sec, 1.651 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283162, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 309, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (329.07, 153.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677176 reads in 166.938 CPU sec, 5.290 real sec
sorting block 139, #reads = 1071269 mem = 536878208 peak = 1204248576
[M::mem_process_seqs] Processed 680678 reads in 102.337 CPU sec, 3.256 real sec
sorting block 140, #reads = 1067694 mem = 536880200 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283404, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 316, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (334.73, 154.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678396 sequences (100000173 bp) in 2.435 CPU sec, 2.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282371, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 323, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886)
[M::mem_pestat] mean and std.dev: (340.10, 154.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678164 sequences (100000038 bp) in 1.610 CPU sec, 1.614 real sec...
[M::mem_process_seqs] Processed 679642 reads in 141.433 CPU sec, 4.463 real sec
[M::mem_process_seqs] Processed 678926 reads in 90.292 CPU sec, 2.852 real sec
sorting block 141, #reads = 1077646 mem = 536879824 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282928, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 325, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (342.43, 155.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678024 sequences (100000159 bp) in 2.267 CPU sec, 2.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282615, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 327, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (343.23, 155.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677682 sequences (100000191 bp) in 1.780 CPU sec, 1.776 real sec...
[M::mem_process_seqs] Processed 678396 reads in 136.069 CPU sec, 4.288 real sec
[M::mem_process_seqs] Processed 678164 reads in 87.952 CPU sec, 2.769 real sec
sorting block 142, #reads = 1079086 mem = 536878432 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282711, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (345.99, 156.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677320 sequences (100000132 bp) in 2.231 CPU sec, 2.276 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282063, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (347.16, 156.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677188 sequences (100000044 bp) in 1.620 CPU sec, 1.622 real sec...
[M::mem_process_seqs] Processed 678024 reads in 136.397 CPU sec, 4.307 real sec
sorting block 143, #reads = 1078478 mem = 536878419 peak = 1204248576
[M::mem_process_seqs] Processed 677682 reads in 88.339 CPU sec, 2.786 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282038, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (346.95, 155.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677378 sequences (100000148 bp) in 2.503 CPU sec, 2.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281965, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 333, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (347.59, 154.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676856 sequences (100000296 bp) in 1.640 CPU sec, 1.640 real sec...
[M::mem_process_seqs] Processed 677320 reads in 138.570 CPU sec, 4.367 real sec
sorting block 144, #reads = 1078377 mem = 536878574 peak = 1204248576
[M::mem_process_seqs] Processed 677188 reads in 89.801 CPU sec, 2.829 real sec
sorting block 145, #reads = 1077952 mem = 536880231 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282485, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 332, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (347.18, 154.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676392 sequences (100000222 bp) in 2.201 CPU sec, 2.246 real sec...
[M::process] read 676624 sequences (100000232 bp) in 1.590 CPU sec, 1.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281527, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (351.41, 157.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677378 reads in 137.516 CPU sec, 4.344 real sec
[M::mem_process_seqs] Processed 676856 reads in 87.270 CPU sec, 2.763 real sec
sorting block 146, #reads = 1078004 mem = 536881785 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281536, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 339, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (353.33, 156.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676326 sequences (100000266 bp) in 2.244 CPU sec, 2.287 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282193, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 339, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (353.60, 157.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676246 sequences (100000086 bp) in 1.630 CPU sec, 1.626 real sec...
[M::mem_process_seqs] Processed 676392 reads in 134.169 CPU sec, 4.234 real sec
[M::mem_process_seqs] Processed 676624 reads in 86.868 CPU sec, 2.742 real sec
sorting block 147, #reads = 1078200 mem = 536879069 peak = 1204248576
[M::process] read 677144 sequences (100000204 bp) in 2.289 CPU sec, 2.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282171, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 343, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (356.85, 158.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677148 sequences (100000152 bp) in 1.620 CPU sec, 1.622 real sec...
[M::mem_process_seqs] Processed 676246 reads in 73.723 CPU sec, 2.309 real sec
sorting block 148, #reads = 1078087 mem = 536882776 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281431, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 342, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (355.92, 158.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282342, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 333, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (348.46, 155.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676326 reads in 206.554 CPU sec, 6.516 real sec
[M::process] read 676566 sequences (100000170 bp) in 1.813 CPU sec, 1.817 real sec...
[M::mem_process_seqs] Processed 677144 reads in 100.804 CPU sec, 3.188 real sec
sorting block 149, #reads = 1078583 mem = 536877517 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281670, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (347.75, 156.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676928 sequences (100000127 bp) in 2.356 CPU sec, 2.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282274, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 338, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (352.69, 156.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676398 sequences (100000147 bp) in 1.627 CPU sec, 1.626 real sec...
[M::mem_process_seqs] Processed 677148 reads in 137.919 CPU sec, 4.354 real sec
[M::mem_process_seqs] Processed 676566 reads in 89.538 CPU sec, 2.825 real sec
sorting block 150, #reads = 1077990 mem = 536879669 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282081, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 339, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (354.15, 158.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675844 sequences (100000107 bp) in 2.117 CPU sec, 2.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281986, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 342, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (356.68, 159.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675592 sequences (100000213 bp) in 1.630 CPU sec, 1.627 real sec...
[M::mem_process_seqs] Processed 676928 reads in 140.858 CPU sec, 4.452 real sec
sorting block 151, #reads = 1077650 mem = 536878407 peak = 1204248576
[M::mem_process_seqs] Processed 676398 reads in 90.872 CPU sec, 2.872 real sec
sorting block 152, #reads = 1076113 mem = 536876244 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281472, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 344, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (358.21, 158.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675722 sequences (100000265 bp) in 2.417 CPU sec, 2.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281640, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (361.13, 160.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675844 reads in 138.392 CPU sec, 4.360 real sec
[M::process] read 675468 sequences (100000243 bp) in 1.789 CPU sec, 1.790 real sec...
[M::mem_process_seqs] Processed 675592 reads in 90.491 CPU sec, 2.841 real sec
sorting block 153, #reads = 1076492 mem = 536878036 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281923, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (362.56, 160.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675404 sequences (100000185 bp) in 2.153 CPU sec, 2.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281043, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 347, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (362.45, 160.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675428 sequences (100000077 bp) in 1.660 CPU sec, 1.665 real sec...
[M::mem_process_seqs] Processed 675722 reads in 139.717 CPU sec, 4.413 real sec
[M::mem_process_seqs] Processed 675468 reads in 92.204 CPU sec, 2.904 real sec
sorting block 154, #reads = 1076246 mem = 536887060 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281891, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 347, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (362.25, 160.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675640 sequences (100000152 bp) in 2.101 CPU sec, 2.142 real sec...
[M::process] read 676102 sequences (100000098 bp) in 1.670 CPU sec, 1.665 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281775, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (363.59, 161.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675404 reads in 139.768 CPU sec, 4.414 real sec
sorting block 155, #reads = 1075661 mem = 536880824 peak = 1204248576
[M::mem_process_seqs] Processed 675428 reads in 90.154 CPU sec, 2.856 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282132, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (362.06, 161.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675404 sequences (100000180 bp) in 2.117 CPU sec, 2.165 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282409, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (357.74, 160.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675518 sequences (100000056 bp) in 1.660 CPU sec, 1.661 real sec...
[M::mem_process_seqs] Processed 675640 reads in 140.816 CPU sec, 4.452 real sec
sorting block 156, #reads = 1076566 mem = 536879644 peak = 1204248576
[M::mem_process_seqs] Processed 676102 reads in 91.022 CPU sec, 2.868 real sec
sorting block 157, #reads = 1075500 mem = 536877861 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281530, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (361.51, 159.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675110 sequences (100000125 bp) in 2.331 CPU sec, 2.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281467, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (360.29, 159.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675168 sequences (100000180 bp) in 1.670 CPU sec, 1.665 real sec...
[M::mem_process_seqs] Processed 675404 reads in 141.571 CPU sec, 4.470 real sec
[M::mem_process_seqs] Processed 675518 reads in 92.588 CPU sec, 2.917 real sec
sorting block 158, #reads = 1075861 mem = 536878216 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281175, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (362.55, 160.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675400 sequences (100000272 bp) in 2.016 CPU sec, 2.049 real sec...
[M::process] read 675224 sequences (100000184 bp) in 1.660 CPU sec, 1.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281392, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 347, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (362.93, 161.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675110 reads in 144.246 CPU sec, 4.563 real sec
[M::mem_process_seqs] Processed 675168 reads in 94.449 CPU sec, 2.985 real sec
sorting block 159, #reads = 1074630 mem = 536879402 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281836, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (361.88, 161.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675126 sequences (100000239 bp) in 2.134 CPU sec, 2.172 real sec...
[M::process] read 677318 sequences (100000099 bp) in 1.720 CPU sec, 1.724 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281604, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (364.93, 163.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675400 reads in 146.591 CPU sec, 4.635 real sec
sorting block 160, #reads = 1073318 mem = 536879132 peak = 1204248576
[M::mem_process_seqs] Processed 675224 reads in 94.186 CPU sec, 2.983 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282174, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 349, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (366.15, 163.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685766 sequences (100000257 bp) in 2.134 CPU sec, 2.172 real sec...
[M::process] read 683492 sequences (100000270 bp) in 1.730 CPU sec, 1.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281793, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (351.55, 160.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675126 reads in 162.715 CPU sec, 5.155 real sec
sorting block 161, #reads = 1073521 mem = 536880977 peak = 1204248576
[M::mem_process_seqs] Processed 677318 reads in 111.731 CPU sec, 3.541 real sec
sorting block 162, #reads = 1066157 mem = 536881382 peak = 1204248576
[M::process] read 681508 sequences (100000158 bp) in 2.411 CPU sec, 2.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284974, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 278, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (299.40, 140.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680660 sequences (100000192 bp) in 1.710 CPU sec, 1.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284448, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 295, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841)
[M::mem_pestat] mean and std.dev: (314.75, 146.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685766 reads in 167.274 CPU sec, 5.291 real sec
[M::mem_process_seqs] Processed 683492 reads in 185.303 CPU sec, 5.877 real sec
sorting block 163, #reads = 1069541 mem = 536878758 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283498, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 307, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (325.17, 149.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680180 sequences (100000244 bp) in 2.306 CPU sec, 2.345 real sec...
[M::process] read 679456 sequences (100000158 bp) in 1.710 CPU sec, 1.706 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283837, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 312, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (326.93, 147.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681508 reads in 159.635 CPU sec, 5.050 real sec
sorting block 164, #reads = 1070592 mem = 536878900 peak = 1204248576
[M::process] read 678610 sequences (100000063 bp) in 1.945 CPU sec, 1.952 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283252, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 315, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (332.24, 151.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680180 reads in 89.886 CPU sec, 3.039 real sec
[M::mem_process_seqs] Processed 680660 reads in 215.347 CPU sec, 7.026 real sec
sorting block 165, #reads = 1071254 mem = 536878849 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282375, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 319, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (335.10, 151.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678170 sequences (100000004 bp) in 2.126 CPU sec, 2.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283050, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (341.67, 152.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677942 sequences (100000089 bp) in 1.660 CPU sec, 1.659 real sec...
[M::mem_process_seqs] Processed 679456 reads in 170.657 CPU sec, 5.404 real sec
[M::mem_process_seqs] Processed 678610 reads in 101.189 CPU sec, 3.191 real sec
sorting block 166, #reads = 1071417 mem = 536877737 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282229, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (342.12, 152.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677794 sequences (100000125 bp) in 2.364 CPU sec, 2.432 real sec...
[M::process] read 677058 sequences (100000297 bp) in 1.690 CPU sec, 1.689 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283177, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (345.55, 153.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678170 reads in 152.116 CPU sec, 4.806 real sec
sorting block 167, #reads = 1072219 mem = 536877510 peak = 1204248576
[M::process] read 676890 sequences (100000239 bp) in 1.917 CPU sec, 1.920 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282643, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (348.11, 154.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677794 reads in 87.278 CPU sec, 2.948 real sec
[M::mem_process_seqs] Processed 677942 reads in 201.480 CPU sec, 6.570 real sec
sorting block 168, #reads = 1071539 mem = 536876801 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282445, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 338, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (350.76, 153.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676834 sequences (100000290 bp) in 2.337 CPU sec, 2.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282192, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 341, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (353.02, 154.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676840 sequences (100000133 bp) in 1.680 CPU sec, 1.681 real sec...
[M::mem_process_seqs] Processed 677058 reads in 167.435 CPU sec, 5.289 real sec
sorting block 169, #reads = 1073012 mem = 536878082 peak = 1204248576
[M::mem_process_seqs] Processed 676890 reads in 100.196 CPU sec, 3.159 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282402, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 339, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (352.65, 156.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675938 sequences (100000279 bp) in 2.198 CPU sec, 2.264 real sec...
[M::process] read 675698 sequences (100000247 bp) in 1.660 CPU sec, 1.659 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282158, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 337, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (351.14, 155.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676834 reads in 148.874 CPU sec, 4.704 real sec
sorting block 170, #reads = 1070887 mem = 536879990 peak = 1204248576
[M::mem_process_seqs] Processed 676840 reads in 97.610 CPU sec, 3.095 real sec
sorting block 171, #reads = 1072440 mem = 536881087 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281534, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 345, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (357.92, 156.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675922 sequences (100000069 bp) in 2.255 CPU sec, 2.308 real sec...
[M::process] read 675720 sequences (100000002 bp) in 1.720 CPU sec, 1.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281786, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (360.77, 157.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675938 reads in 146.160 CPU sec, 4.613 real sec
[M::process] read 675344 sequences (100000044 bp) in 1.712 CPU sec, 1.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282491, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (360.46, 158.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675922 reads in 84.312 CPU sec, 2.829 real sec
sorting block 172, #reads = 1073021 mem = 536880905 peak = 1204248576
[M::mem_process_seqs] Processed 675698 reads in 194.115 CPU sec, 6.308 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282119, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 347, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (359.53, 156.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 173, #reads = 1074362 mem = 536879929 peak = 1204248576
[M::process] read 675352 sequences (100000184 bp) in 2.417 CPU sec, 2.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281949, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (358.38, 156.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675720 reads in 162.120 CPU sec, 5.122 real sec
[M::process] read 675326 sequences (100000293 bp) in 1.657 CPU sec, 1.667 real sec...
[M::mem_process_seqs] Processed 675344 reads in 96.196 CPU sec, 3.023 real sec
sorting block 174, #reads = 1074055 mem = 536881676 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281786, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 342, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (356.83, 155.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675578 sequences (100000203 bp) in 2.526 CPU sec, 2.603 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281325, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (360.09, 157.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675294 sequences (100000142 bp) in 1.630 CPU sec, 1.630 real sec...
[M::mem_process_seqs] Processed 675352 reads in 144.332 CPU sec, 4.549 real sec
[M::mem_process_seqs] Processed 675326 reads in 93.449 CPU sec, 2.945 real sec
sorting block 175, #reads = 1073577 mem = 536879125 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281252, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (360.56, 159.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675254 sequences (100000117 bp) in 2.247 CPU sec, 2.279 real sec...
[M::process] read 675482 sequences (100000098 bp) in 1.630 CPU sec, 1.636 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281559, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 344, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (359.32, 157.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675578 reads in 142.215 CPU sec, 4.490 real sec
sorting block 176, #reads = 1074528 mem = 536879573 peak = 1204248576
[M::mem_process_seqs] Processed 675294 reads in 92.858 CPU sec, 2.941 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281625, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (360.60, 158.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675058 sequences (100000113 bp) in 1.972 CPU sec, 2.018 real sec...
[M::process] read 674752 sequences (100000251 bp) in 1.610 CPU sec, 1.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281656, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (358.87, 157.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675254 reads in 137.807 CPU sec, 4.359 real sec
sorting block 177, #reads = 1074948 mem = 536882005 peak = 1204248576
[M::mem_process_seqs] Processed 675482 reads in 90.698 CPU sec, 2.865 real sec
sorting block 178, #reads = 1074594 mem = 536879044 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281832, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (362.09, 158.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674916 sequences (100000225 bp) in 2.291 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281476, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 350, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (365.30, 159.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674824 sequences (100000220 bp) in 1.650 CPU sec, 1.650 real sec...
[M::mem_process_seqs] Processed 675058 reads in 140.745 CPU sec, 4.442 real sec
[M::mem_process_seqs] Processed 674752 reads in 92.404 CPU sec, 2.914 real sec
sorting block 179, #reads = 1075017 mem = 536880096 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281706, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (365.44, 160.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674638 sequences (100000134 bp) in 2.009 CPU sec, 2.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281287, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (365.21, 161.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674638 sequences (100000181 bp) in 1.610 CPU sec, 1.608 real sec...
[M::mem_process_seqs] Processed 674916 reads in 137.524 CPU sec, 4.347 real sec
[M::mem_process_seqs] Processed 674824 reads in 88.865 CPU sec, 2.809 real sec
sorting block 180, #reads = 1076213 mem = 536879327 peak = 1204248576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281069, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (519, 547, 1264)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2754)
[M::mem_pestat] mean and std.dev: (545.62, 305.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3499)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (367.41, 162.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674424 sequences (100000045 bp) in 2.278 CPU sec, 2.316 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280941, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 353, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (368.57, 162.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674418 sequences (100000229 bp) in 1.640 CPU sec, 1.638 real sec...
[M::mem_process_seqs] Processed 674638 reads in 135.650 CPU sec, 4.279 real sec
sorting block 181, #reads = 1076071 mem = 536879149 peak = 1204248576
[M::mem_process_seqs] Processed 674638 reads in 87.743 CPU sec, 2.767 real sec
[M::process] read 168300 sequences (24875840 bp) in 0.759 CPU sec, 0.769 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280788, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 354, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (370.36, 163.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 70127, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 337, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (354.04, 158.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280972, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 355, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (371.11, 164.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (52, 80, 115)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 241)
[M::mem_pestat] mean and std.dev: (61.44, 37.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 304)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674424 reads in 150.515 CPU sec, 4.856 real sec
[M::mem_process_seqs] Processed 168300 reads in 94.609 CPU sec, 3.056 real sec
sorting block 182, #reads = 1076813 mem = 536879830 peak = 1204248576
[M::mem_process_seqs] Processed 674418 reads in 109.502 CPU sec, 3.557 real sec
sorting block 183, #reads = 1075286 mem = 536879800 peak = 1231007744
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 197642602
overall: 69808062464 mem 24202.472 user 569.609 sys 857.648 real
sorting block 184, #reads = 275469 mem = 137579488 peak = 1231007744
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_bR4-T1-TDNA-1_A23MHWWLT4_1.L005\tPU:L005\tSM:659_bR4-T1-TDNA-1_A23MHWWLT4_1\tLB:659_bR4-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_bR4-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_bR4-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 857.684 sec; CPU: 24772.089 sec
uniq header count 1
merging 185 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 11178594303
output reads: 198460596
bam_mem_sort: 185 calls 88.418 user 1.792 sys 91.451 real
bam_write: 185 calls 112.908 user 4.762 sys 132.519 real
execute: 1 calls 47.056 user 1.627 sys 934.830 real
merge_files: 1 calls 47.015 user 1.602 sys 76.818 real
parse_chunk: 2949 calls 151.100 user 31.586 sys 190.925 real
read_chunk: 7944 calls 73.149 user 53.225 sys 783.569 real
sort_block: 1 calls 49.987 user 52.701 sys 750.955 real
write_chunk: 4993 calls 46.704 user 1.244 sys 75.861 real
overall: 3325456384 mem 2999.499 user 108.494 sys 934.850 real
ls: cannot access '*.cram': No such file or directory