This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1613.164 CPU sec, 834.254 real sec
[M::process] read 702558 sequences (100000022 bp) in 0.993 CPU sec, 0.996 real sec...
[M::process] read 700980 sequences (100000238 bp) in 1.264 CPU sec, 1.316 real sec...
[M::process] read 700216 sequences (100000073 bp) in 1.230 CPU sec, 1.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292140, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (239.07, 113.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 781)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 249, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (135, 365)
[M::mem_pestat] mean and std.dev: (248.20, 35.54)
[M::mem_pestat] low and high boundaries for proper pairs: (89, 411)
[M::mem_pestat] skip orientation RR
[M::process] read 699794 sequences (100000179 bp) in 1.180 CPU sec, 1.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291978, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.54, 115.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291334, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (245.77, 117.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702558 reads in 208.708 CPU sec, 6.680 real sec
[M::mem_process_seqs] Processed 700980 reads in 136.735 CPU sec, 4.386 real sec
[M::mem_process_seqs] Processed 700216 reads in 148.362 CPU sec, 4.775 real sec
sorting block 0, #reads = 1096291 mem = 536877648 peak = 603394048
[M::process] read 698970 sequences (100000152 bp) in 1.820 CPU sec, 1.963 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291801, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.05, 118.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698624 sequences (100000298 bp) in 1.461 CPU sec, 1.502 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 291121, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 259, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (48, 483)
[M::mem_pestat] mean and std.dev: (238.38, 65.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 570)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.39, 119.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699794 reads in 131.083 CPU sec, 4.202 real sec
sorting block 1, #reads = 1094298 mem = 536882091 peak = 822022144
[M::mem_process_seqs] Processed 698970 reads in 83.576 CPU sec, 2.672 real sec
[M::process] read 697676 sequences (100000086 bp) in 1.452 CPU sec, 1.498 real sec...
sorting block 2, #reads = 1095384 mem = 536878785 peak = 882171904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290916, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.45, 119.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697820 sequences (100000116 bp) in 1.799 CPU sec, 1.917 real sec...
[M::process] read 697772 sequences (100000186 bp) in 1.260 CPU sec, 1.258 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290760, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.37, 120.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698624 reads in 129.519 CPU sec, 4.125 real sec
[M::mem_process_seqs] Processed 697676 reads in 81.143 CPU sec, 2.587 real sec
sorting block 3, #reads = 1093902 mem = 536885211 peak = 882171904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290551, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.16, 120.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697826 sequences (100000115 bp) in 1.842 CPU sec, 1.982 real sec...
[M::process] read 698262 sequences (100000245 bp) in 1.310 CPU sec, 1.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290995, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 246, 716)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1798)
[M::mem_pestat] mean and std.dev: (282.11, 175.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2339)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.45, 119.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697820 reads in 130.727 CPU sec, 4.161 real sec
[M::mem_process_seqs] Processed 697772 reads in 81.607 CPU sec, 2.590 real sec
sorting block 4, #reads = 1093933 mem = 536881102 peak = 882171904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290899, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 218, 639)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1623)
[M::mem_pestat] mean and std.dev: (304.90, 275.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2115)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (249.84, 118.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 230, 1300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3500)
[M::mem_pestat] mean and std.dev: (779.30, 829.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4600)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697378 sequences (100000136 bp) in 1.981 CPU sec, 2.099 real sec...
[M::process] read 697358 sequences (100000035 bp) in 1.360 CPU sec, 1.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290700, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.33, 118.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697826 reads in 133.009 CPU sec, 4.235 real sec
sorting block 5, #reads = 1093633 mem = 536880965 peak = 882171904
[M::mem_process_seqs] Processed 698262 reads in 83.392 CPU sec, 2.649 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290357, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.57, 118.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 6, #reads = 1093006 mem = 536880890 peak = 882171904
[M::process] read 696032 sequences (100000076 bp) in 2.150 CPU sec, 2.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290222, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.60, 118.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696492 sequences (100000147 bp) in 1.370 CPU sec, 1.364 real sec...
[M::mem_process_seqs] Processed 697378 reads in 132.937 CPU sec, 4.235 real sec
[M::mem_process_seqs] Processed 697358 reads in 82.984 CPU sec, 2.633 real sec
sorting block 7, #reads = 1093129 mem = 536878084 peak = 882171904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290172, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (254.15, 119.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696090 sequences (100000164 bp) in 2.044 CPU sec, 2.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290419, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (237, 237, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (264.45, 83.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 745)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.20, 119.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 244, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477)
[M::mem_pestat] mean and std.dev: (201.44, 75.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 590)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 695532 sequences (100000098 bp) in 1.440 CPU sec, 1.431 real sec...
[M::mem_process_seqs] Processed 696032 reads in 132.749 CPU sec, 4.231 real sec
[M::mem_process_seqs] Processed 696492 reads in 83.541 CPU sec, 2.644 real sec
sorting block 8, #reads = 1092726 mem = 536882990 peak = 882171904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290121, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (253.02, 118.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695238 sequences (100000140 bp) in 2.336 CPU sec, 2.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290095, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (255.26, 119.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696090 reads in 132.406 CPU sec, 4.220 real sec
[M::process] read 698488 sequences (100000080 bp) in 1.480 CPU sec, 1.479 real sec...
[M::mem_process_seqs] Processed 695532 reads in 83.389 CPU sec, 2.648 real sec
sorting block 9, #reads = 1092492 mem = 536883061 peak = 882171904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290002, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 234, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (256.89, 121.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701708 sequences (100000014 bp) in 2.312 CPU sec, 2.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291096, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.17, 118.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695238 reads in 133.244 CPU sec, 4.243 real sec
[M::process] read 700762 sequences (100000177 bp) in 1.533 CPU sec, 1.535 real sec...
sorting block 10, #reads = 1092736 mem = 536879622 peak = 882171904
[M::mem_process_seqs] Processed 698488 reads in 84.303 CPU sec, 2.665 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292504, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.36, 115.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (285, 287, 805)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1845)
[M::mem_pestat] mean and std.dev: (461.78, 331.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2365)
[M::mem_pestat] skip orientation RR
sorting block 11, #reads = 1092753 mem = 536879317 peak = 911208448
[M::process] read 700100 sequences (100000138 bp) in 2.500 CPU sec, 2.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291697, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.69, 117.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 272, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (348.00, 266.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1413)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701708 reads in 132.426 CPU sec, 4.219 real sec
[M::mem_process_seqs] Processed 700762 reads in 83.321 CPU sec, 2.631 real sec
[M::process] read 699298 sequences (100000134 bp) in 1.865 CPU sec, 1.916 real sec...
sorting block 12, #reads = 1096656 mem = 536879244 peak = 911208448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291677, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.17, 118.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699082 sequences (100000221 bp) in 2.416 CPU sec, 2.583 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291363, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 384, 1000)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2612)
[M::mem_pestat] mean and std.dev: (707.20, 735.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3649)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (248.96, 119.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700100 reads in 132.222 CPU sec, 4.222 real sec
[M::mem_process_seqs] Processed 699298 reads in 83.169 CPU sec, 2.631 real sec
[M::process] read 698518 sequences (100000165 bp) in 2.070 CPU sec, 2.179 real sec...
sorting block 13, #reads = 1095660 mem = 536881593 peak = 911208448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291023, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.12, 120.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 258, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (341.42, 231.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RR
[M::process] read 698192 sequences (100000059 bp) in 2.338 CPU sec, 2.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291010, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.10, 121.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699082 reads in 133.503 CPU sec, 4.268 real sec
[M::mem_process_seqs] Processed 698518 reads in 83.655 CPU sec, 2.653 real sec
sorting block 14, #reads = 1095197 mem = 536878436 peak = 911208448
[M::process] read 697898 sequences (100000074 bp) in 2.038 CPU sec, 2.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291053, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.58, 121.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 352, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846)
[M::mem_pestat] mean and std.dev: (333.38, 78.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RR
sorting block 15, #reads = 1095071 mem = 536878334 peak = 911208448
[M::process] read 697868 sequences (100000146 bp) in 2.043 CPU sec, 2.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290858, 9, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 411, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (297.25, 133.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.42, 120.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698192 reads in 134.156 CPU sec, 4.281 real sec
[M::mem_process_seqs] Processed 697898 reads in 84.444 CPU sec, 2.670 real sec
[M::process] read 698432 sequences (100000178 bp) in 1.647 CPU sec, 1.701 real sec...
sorting block 16, #reads = 1094167 mem = 536878306 peak = 911208448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290838, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (249.69, 118.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698216 sequences (100000075 bp) in 2.268 CPU sec, 2.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291089, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.19, 119.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697868 reads in 134.492 CPU sec, 4.293 real sec
[M::mem_process_seqs] Processed 698432 reads in 84.809 CPU sec, 2.680 real sec
[M::process] read 698044 sequences (100000025 bp) in 1.623 CPU sec, 1.654 real sec...
sorting block 17, #reads = 1092791 mem = 536877319 peak = 911208448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 291034, 8, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 284, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1287)
[M::mem_pestat] mean and std.dev: (329.46, 192.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1657)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.81, 118.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 291, 1170)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3120)
[M::mem_pestat] mean and std.dev: (404.50, 387.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4095)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697272 sequences (100000153 bp) in 2.232 CPU sec, 2.349 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290717, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 282, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692)
[M::mem_pestat] mean and std.dev: (262.50, 158.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.95, 119.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (378, 736, 1049)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2391)
[M::mem_pestat] mean and std.dev: (746.80, 532.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3062)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698216 reads in 134.193 CPU sec, 4.282 real sec
[M::process] read 697660 sequences (100000160 bp) in 1.463 CPU sec, 1.476 real sec...
[M::mem_process_seqs] Processed 698044 reads in 84.107 CPU sec, 2.659 real sec
sorting block 18, #reads = 1093416 mem = 536877592 peak = 911208448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290825, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.84, 119.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697186 sequences (100000167 bp) in 2.309 CPU sec, 2.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290690, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.33, 119.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697272 reads in 132.870 CPU sec, 4.228 real sec
[M::process] read 696696 sequences (100000157 bp) in 1.653 CPU sec, 1.671 real sec...
sorting block 19, #reads = 1093596 mem = 536881001 peak = 911208448
[M::mem_process_seqs] Processed 697660 reads in 83.432 CPU sec, 2.636 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290352, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.54, 120.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 20, #reads = 1093209 mem = 536877262 peak = 969310208
[M::process] read 695854 sequences (100000044 bp) in 2.508 CPU sec, 2.652 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290283, 8, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.81, 119.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697186 reads in 132.861 CPU sec, 4.235 real sec
[M::mem_process_seqs] Processed 696696 reads in 83.792 CPU sec, 2.646 real sec
[M::process] read 695890 sequences (100000189 bp) in 1.886 CPU sec, 1.950 real sec...
sorting block 21, #reads = 1092922 mem = 536880271 peak = 969310208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290084, 8, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 263, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451)
[M::mem_pestat] mean and std.dev: (233.44, 83.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 568)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (254.94, 121.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 307, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (160, 410)
[M::mem_pestat] mean and std.dev: (264.55, 56.19)
[M::mem_pestat] low and high boundaries for proper pairs: (40, 489)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 702558 sequences (100000097 bp) in 2.286 CPU sec, 2.415 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289646, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (256.83, 123.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695854 reads in 134.699 CPU sec, 4.304 real sec
[M::mem_process_seqs] Processed 695890 reads in 85.379 CPU sec, 2.702 real sec
[M::process] read 701478 sequences (100000043 bp) in 1.845 CPU sec, 1.882 real sec...
sorting block 22, #reads = 1092924 mem = 536878347 peak = 969310208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292304, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (240.73, 114.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700352 sequences (100000098 bp) in 2.393 CPU sec, 2.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292168, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.47, 115.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702558 reads in 135.488 CPU sec, 4.317 real sec
[M::mem_process_seqs] Processed 701478 reads in 85.009 CPU sec, 2.689 real sec
[M::process] read 699670 sequences (100000092 bp) in 1.822 CPU sec, 1.880 real sec...
sorting block 23, #reads = 1092964 mem = 536876793 peak = 969310208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291541, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (245.96, 117.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 680, 1367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3663)
[M::mem_pestat] mean and std.dev: (712.00, 553.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4811)
[M::mem_pestat] skip orientation RR
sorting block 24, #reads = 1095346 mem = 536879344 peak = 969310208
[M::process] read 698650 sequences (100000108 bp) in 2.417 CPU sec, 2.515 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291456, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.72, 118.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700352 reads in 133.821 CPU sec, 4.272 real sec
[M::mem_process_seqs] Processed 699670 reads in 84.421 CPU sec, 2.664 real sec
[M::process] read 698336 sequences (100000102 bp) in 1.889 CPU sec, 1.967 real sec...
sorting block 25, #reads = 1094487 mem = 536878140 peak = 969310208
[M::process] read 698126 sequences (100000219 bp) in 2.252 CPU sec, 2.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290802, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.67, 119.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698336 reads in 73.063 CPU sec, 2.307 real sec
[M::process] read 697800 sequences (100000012 bp) in 1.755 CPU sec, 1.827 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291073, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.72, 118.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290984, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.86, 121.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698650 reads in 202.200 CPU sec, 6.425 real sec
sorting block 26, #reads = 1093526 mem = 536878454 peak = 969310208
[M::mem_process_seqs] Processed 698126 reads in 94.796 CPU sec, 2.997 real sec
[M::process] read 697742 sequences (100000079 bp) in 2.147 CPU sec, 2.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290998, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.89, 121.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (131, 314, 1525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4313)
[M::mem_pestat] mean and std.dev: (704.64, 602.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5707)
[M::mem_pestat] skip orientation RR
sorting block 27, #reads = 1093948 mem = 536890694 peak = 969310208
[M::process] read 697572 sequences (100000024 bp) in 2.480 CPU sec, 2.593 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290681, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.54, 120.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 204, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1168)
[M::mem_pestat] mean and std.dev: (245.78, 119.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1497)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697800 reads in 130.166 CPU sec, 4.160 real sec
[M::mem_process_seqs] Processed 697742 reads in 83.650 CPU sec, 2.646 real sec
[M::process] read 697856 sequences (100000177 bp) in 2.081 CPU sec, 2.187 real sec...
sorting block 28, #reads = 1092294 mem = 536881323 peak = 969310208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290434, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 197, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (255.20, 140.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1071)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.14, 119.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697496 sequences (100000242 bp) in 2.099 CPU sec, 2.189 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290681, 7, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 211, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586)
[M::mem_pestat] mean and std.dev: (234.67, 88.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 716)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.89, 119.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697572 reads in 131.690 CPU sec, 4.198 real sec
[M::mem_process_seqs] Processed 697856 reads in 83.419 CPU sec, 2.638 real sec
sorting block 29, #reads = 1093175 mem = 536879201 peak = 969310208
[M::process] read 697272 sequences (100000216 bp) in 1.998 CPU sec, 2.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290602, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.20, 118.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697196 sequences (100000223 bp) in 2.293 CPU sec, 2.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290167, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.48, 119.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697496 reads in 131.140 CPU sec, 4.183 real sec
[M::mem_process_seqs] Processed 697272 reads in 83.384 CPU sec, 2.633 real sec
sorting block 30, #reads = 1092569 mem = 536887141 peak = 969310208
[M::process] read 696884 sequences (100000126 bp) in 2.033 CPU sec, 2.133 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290371, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (257, 315, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 629)
[M::mem_pestat] mean and std.dev: (289.91, 76.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.43, 119.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 31, #reads = 1093650 mem = 536878806 peak = 969310208
[M::process] read 696578 sequences (100000107 bp) in 2.205 CPU sec, 2.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 290015, 6, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 272, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184)
[M::mem_pestat] mean and std.dev: (360.81, 224.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1522)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.34, 120.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697196 reads in 130.946 CPU sec, 4.180 real sec
[M::mem_process_seqs] Processed 696884 reads in 83.058 CPU sec, 2.623 real sec
[M::process] read 696102 sequences (100000183 bp) in 1.996 CPU sec, 2.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290239, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.72, 120.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 32, #reads = 1092122 mem = 536878927 peak = 969310208
[M::process] read 695474 sequences (100000099 bp) in 2.219 CPU sec, 2.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290103, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.06, 121.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696578 reads in 129.661 CPU sec, 4.137 real sec
[M::mem_process_seqs] Processed 696102 reads in 82.745 CPU sec, 2.615 real sec
[M::process] read 700158 sequences (100000055 bp) in 2.012 CPU sec, 2.098 real sec...
sorting block 33, #reads = 1093058 mem = 536884592 peak = 969310208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 289584, 7, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (226, 283, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (252.20, 88.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 934)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 233, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (256.22, 121.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701940 sequences (100000265 bp) in 2.129 CPU sec, 2.214 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291371, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (245.55, 117.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695474 reads in 131.337 CPU sec, 4.188 real sec
[M::mem_process_seqs] Processed 700158 reads in 84.269 CPU sec, 2.663 real sec
sorting block 34, #reads = 1091363 mem = 536880430 peak = 969310208
[M::process] read 700882 sequences (100000206 bp) in 1.824 CPU sec, 1.899 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291870, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.91, 116.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700744 sequences (100000030 bp) in 2.247 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291965, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (244.08, 117.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (298, 464, 1335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3409)
[M::mem_pestat] mean and std.dev: (698.15, 527.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4446)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701940 reads in 130.290 CPU sec, 4.151 real sec
sorting block 35, #reads = 1094082 mem = 536882369 peak = 969310208
[M::mem_process_seqs] Processed 700882 reads in 82.223 CPU sec, 2.599 real sec
[M::process] read 700070 sequences (100000064 bp) in 1.723 CPU sec, 1.781 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291398, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.92, 118.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1096651 mem = 536876986 peak = 995065856
[M::process] read 699388 sequences (100000091 bp) in 2.224 CPU sec, 2.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291667, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 347, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1182)
[M::mem_pestat] mean and std.dev: (404.33, 293.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1580)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.86, 120.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700744 reads in 129.750 CPU sec, 4.133 real sec
[M::mem_process_seqs] Processed 700070 reads in 82.038 CPU sec, 2.590 real sec
[M::process] read 699662 sequences (100000229 bp) in 1.631 CPU sec, 1.675 real sec...
sorting block 37, #reads = 1095551 mem = 536878254 peak = 995065856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291114, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.12, 120.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698812 sequences (100000103 bp) in 2.297 CPU sec, 2.394 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291300, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.41, 118.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 275, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (158, 368)
[M::mem_pestat] mean and std.dev: (255.86, 32.01)
[M::mem_pestat] low and high boundaries for proper pairs: (116, 410)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699388 reads in 129.108 CPU sec, 4.110 real sec
[M::mem_process_seqs] Processed 699662 reads in 82.614 CPU sec, 2.603 real sec
[M::process] read 698234 sequences (100000258 bp) in 1.680 CPU sec, 1.728 real sec...
sorting block 38, #reads = 1094755 mem = 536880062 peak = 995065856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291276, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.20, 121.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698078 sequences (100000023 bp) in 2.362 CPU sec, 2.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290869, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.42, 121.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698812 reads in 129.504 CPU sec, 4.128 real sec
[M::mem_process_seqs] Processed 698234 reads in 82.564 CPU sec, 2.607 real sec
sorting block 39, #reads = 1095161 mem = 536880854 peak = 995065856
[M::process] read 698028 sequences (100000105 bp) in 1.823 CPU sec, 1.890 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290397, 2, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.31, 120.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 290, 5166)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14994)
[M::mem_pestat] mean and std.dev: (2273.73, 2791.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19908)
[M::mem_pestat] skip orientation RR
sorting block 40, #reads = 1094652 mem = 536878305 peak = 1001254912
[M::process] read 697956 sequences (100000273 bp) in 2.276 CPU sec, 2.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290444, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.29, 119.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698078 reads in 131.411 CPU sec, 4.193 real sec
[M::mem_process_seqs] Processed 698028 reads in 83.473 CPU sec, 2.637 real sec
[M::process] read 697624 sequences (100000165 bp) in 1.861 CPU sec, 1.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290860, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.21, 119.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1092986 mem = 536881051 peak = 1001254912
[M::process] read 697738 sequences (100000081 bp) in 2.273 CPU sec, 2.371 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290602, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 385, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (258.75, 105.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.47, 119.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697956 reads in 130.870 CPU sec, 4.169 real sec
[M::mem_process_seqs] Processed 697624 reads in 83.278 CPU sec, 2.630 real sec
[M::process] read 697036 sequences (100000092 bp) in 1.795 CPU sec, 1.851 real sec...
sorting block 42, #reads = 1093798 mem = 536879975 peak = 1001254912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290800, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 339, 841)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2131)
[M::mem_pestat] mean and std.dev: (367.92, 288.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2776)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.87, 119.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (310, 606, 1381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3523)
[M::mem_pestat] mean and std.dev: (811.92, 725.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4594)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 696422 sequences (100000136 bp) in 2.296 CPU sec, 2.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289658, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.14, 119.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697738 reads in 132.032 CPU sec, 4.204 real sec
[M::mem_process_seqs] Processed 697036 reads in 83.545 CPU sec, 2.635 real sec
[M::process] read 696230 sequences (100000142 bp) in 1.682 CPU sec, 1.724 real sec...
sorting block 43, #reads = 1093511 mem = 536880041 peak = 1001254912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290295, 9, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (252.54, 119.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695672 sequences (100000220 bp) in 2.098 CPU sec, 2.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290102, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (254.07, 120.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696422 reads in 130.453 CPU sec, 4.160 real sec
sorting block 44, #reads = 1092630 mem = 536880881 peak = 1001254912
[M::mem_process_seqs] Processed 696230 reads in 82.674 CPU sec, 2.610 real sec
[M::process] read 699750 sequences (100000103 bp) in 1.703 CPU sec, 1.741 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289831, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (255.92, 121.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1092417 mem = 536880863 peak = 1063084032
[M::process] read 706368 sequences (100000238 bp) in 2.439 CPU sec, 2.574 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290995, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.02, 118.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695672 reads in 133.208 CPU sec, 4.242 real sec
[M::mem_process_seqs] Processed 699750 reads in 86.219 CPU sec, 2.724 real sec
[M::process] read 704784 sequences (100000176 bp) in 1.821 CPU sec, 1.869 real sec...
sorting block 46, #reads = 1091850 mem = 536879001 peak = 1063084032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293384, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 205, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (229.57, 109.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703380 sequences (100000074 bp) in 2.336 CPU sec, 2.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 293032, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (234.31, 113.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 706368 reads in 134.482 CPU sec, 4.284 real sec
[M::mem_process_seqs] Processed 704784 reads in 85.145 CPU sec, 2.692 real sec
[M::process] read 702578 sequences (100000231 bp) in 1.700 CPU sec, 1.724 real sec...
sorting block 47, #reads = 1096198 mem = 536877676 peak = 1063084032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292786, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (237.84, 114.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701846 sequences (100000188 bp) in 2.429 CPU sec, 2.526 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292213, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.43, 116.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703380 reads in 137.823 CPU sec, 4.384 real sec
[M::process] read 700840 sequences (100000275 bp) in 1.575 CPU sec, 1.585 real sec...
[M::mem_process_seqs] Processed 702578 reads in 89.144 CPU sec, 2.816 real sec
sorting block 48, #reads = 1096733 mem = 536882234 peak = 1063084032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292348, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.43, 119.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1095241 mem = 536877526 peak = 1063084032
[M::process] read 700778 sequences (100000237 bp) in 2.303 CPU sec, 2.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291790, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (246.08, 120.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701846 reads in 133.617 CPU sec, 4.252 real sec
[M::process] read 699510 sequences (100000086 bp) in 1.673 CPU sec, 1.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291432, 9, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (246.42, 120.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 212, 220)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (145, 270)
[M::mem_pestat] mean and std.dev: (204.50, 9.81)
[M::mem_pestat] low and high boundaries for proper pairs: (120, 295)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700778 reads in 74.337 CPU sec, 2.420 real sec
[M::process] read 699058 sequences (100000020 bp) in 1.892 CPU sec, 1.910 real sec...
sorting block 50, #reads = 1094117 mem = 536876940 peak = 1063084032
[M::mem_process_seqs] Processed 700840 reads in 183.328 CPU sec, 5.869 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291518, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.50, 121.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698874 sequences (100000282 bp) in 2.514 CPU sec, 2.608 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291416, 8, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 183, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (281.00, 179.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.57, 121.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 319, 667)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1577)
[M::mem_pestat] mean and std.dev: (419.20, 384.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2032)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699510 reads in 147.098 CPU sec, 4.662 real sec
sorting block 51, #reads = 1094592 mem = 536877446 peak = 1063084032
[M::mem_process_seqs] Processed 699058 reads in 85.604 CPU sec, 2.704 real sec
[M::process] read 698472 sequences (100000074 bp) in 2.142 CPU sec, 2.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 290953, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 192, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (209.47, 121.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.46, 120.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 52, #reads = 1093679 mem = 536878430 peak = 1063084032
[M::process] read 698402 sequences (100000261 bp) in 2.351 CPU sec, 2.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290717, 6, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.49, 120.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 203, 8019)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23729)
[M::mem_pestat] mean and std.dev: (2177.69, 3246.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 31584)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698874 reads in 135.802 CPU sec, 4.336 real sec
[M::mem_process_seqs] Processed 698472 reads in 86.671 CPU sec, 2.739 real sec
[M::process] read 697942 sequences (100000287 bp) in 2.219 CPU sec, 2.345 real sec...
sorting block 53, #reads = 1092644 mem = 536880316 peak = 1063084032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291070, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 179, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (160.62, 59.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.48, 120.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698010 sequences (100000060 bp) in 2.224 CPU sec, 2.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290597, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.92, 120.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698402 reads in 137.610 CPU sec, 4.389 real sec
[M::mem_process_seqs] Processed 697942 reads in 87.657 CPU sec, 2.769 real sec
sorting block 54, #reads = 1091858 mem = 536878350 peak = 1063084032
[M::process] read 697620 sequences (100000275 bp) in 2.090 CPU sec, 2.202 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290859, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.17, 119.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697776 sequences (100000292 bp) in 2.276 CPU sec, 2.399 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290493, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.66, 119.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698010 reads in 136.492 CPU sec, 4.352 real sec
[M::mem_process_seqs] Processed 697620 reads in 86.454 CPU sec, 2.731 real sec
sorting block 55, #reads = 1090825 mem = 536877695 peak = 1063084032
[M::process] read 697838 sequences (100000241 bp) in 2.070 CPU sec, 2.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 290597, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.62, 119.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1090926 mem = 536879267 peak = 1063084032
[M::process] read 707544 sequences (100000096 bp) in 2.282 CPU sec, 2.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290580, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.81, 120.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 339, 1636)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4516)
[M::mem_pestat] mean and std.dev: (690.08, 606.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5956)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697776 reads in 138.932 CPU sec, 4.426 real sec
[M::mem_process_seqs] Processed 697838 reads in 88.755 CPU sec, 2.804 real sec
[M::process] read 706012 sequences (100000018 bp) in 2.016 CPU sec, 2.093 real sec...
sorting block 57, #reads = 1091009 mem = 536879759 peak = 1063084032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293457, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 203, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (226.22, 107.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704874 sequences (100000102 bp) in 2.435 CPU sec, 2.577 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293555, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (230.41, 109.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 707544 reads in 148.541 CPU sec, 4.734 real sec
[M::mem_process_seqs] Processed 706012 reads in 94.618 CPU sec, 2.987 real sec
[M::process] read 704602 sequences (100000200 bp) in 1.912 CPU sec, 1.979 real sec...
sorting block 58, #reads = 1092577 mem = 536878864 peak = 1063084032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 293347, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 210, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (234.29, 112.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 301, 1068)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2958)
[M::mem_pestat] mean and std.dev: (533.60, 439.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3903)
[M::mem_pestat] skip orientation RR
[M::process] read 704082 sequences (100000085 bp) in 2.376 CPU sec, 2.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 293347, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (260, 433, 2559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7157)
[M::mem_pestat] mean and std.dev: (1450.79, 1675.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9456)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (235.45, 113.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 286, 1276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3492)
[M::mem_pestat] mean and std.dev: (727.40, 760.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4600)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 704874 reads in 146.669 CPU sec, 4.676 real sec
[M::mem_process_seqs] Processed 704602 reads in 93.018 CPU sec, 2.932 real sec
[M::process] read 702894 sequences (100000223 bp) in 1.811 CPU sec, 1.859 real sec...
sorting block 59, #reads = 1094613 mem = 536877433 peak = 1063084032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292929, 13, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (235.93, 113.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (368, 538, 2175)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5789)
[M::mem_pestat] mean and std.dev: (968.54, 738.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7596)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 60, #reads = 1094357 mem = 536878296 peak = 1063084032
[M::process] read 702118 sequences (100000210 bp) in 2.032 CPU sec, 2.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292546, 6, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 190, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (236.08, 129.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.97, 116.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701590 sequences (100000047 bp) in 1.510 CPU sec, 1.513 real sec...
[M::mem_process_seqs] Processed 704082 reads in 149.632 CPU sec, 4.767 real sec
[M::mem_process_seqs] Processed 702894 reads in 91.969 CPU sec, 2.920 real sec
sorting block 61, #reads = 1092798 mem = 536879254 peak = 1063084032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292295, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 215, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.70, 117.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700498 sequences (100000047 bp) in 2.499 CPU sec, 2.653 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292230, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.48, 118.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702118 reads in 146.639 CPU sec, 4.663 real sec
[M::process] read 699722 sequences (100000028 bp) in 1.685 CPU sec, 1.695 real sec...
[M::mem_process_seqs] Processed 701590 reads in 92.152 CPU sec, 2.911 real sec
sorting block 62, #reads = 1093847 mem = 536881116 peak = 1063084032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291694, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.34, 120.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699544 sequences (100000259 bp) in 2.449 CPU sec, 2.603 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291107, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.75, 120.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700498 reads in 148.872 CPU sec, 4.747 real sec
[M::mem_process_seqs] Processed 699722 reads in 93.472 CPU sec, 2.951 real sec
[M::process] read 698624 sequences (100000050 bp) in 1.789 CPU sec, 1.822 real sec...
sorting block 63, #reads = 1091439 mem = 536877875 peak = 1063084032
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291244, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.17, 119.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698408 sequences (100000161 bp) in 2.678 CPU sec, 2.845 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290869, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.91, 120.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699544 reads in 146.149 CPU sec, 4.657 real sec
sorting block 64, #reads = 1092067 mem = 536879562 peak = 1063084032
[M::mem_process_seqs] Processed 698624 reads in 91.213 CPU sec, 2.876 real sec
[M::process] read 699040 sequences (100000007 bp) in 1.830 CPU sec, 1.879 real sec...
sorting block 65, #reads = 1091348 mem = 536880082 peak = 1096519680
[M::process] read 698122 sequences (100000114 bp) in 2.477 CPU sec, 2.635 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291008, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.77, 120.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 293, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (251.78, 66.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 710)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699040 reads in 75.985 CPU sec, 2.398 real sec
[M::process] read 697998 sequences (100000114 bp) in 2.034 CPU sec, 2.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291210, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.55, 120.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 250, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (263.56, 166.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290860, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.67, 121.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698408 reads in 219.863 CPU sec, 7.000 real sec
[M::mem_process_seqs] Processed 698122 reads in 106.519 CPU sec, 3.376 real sec
sorting block 66, #reads = 1092273 mem = 536879080 peak = 1096519680
[M::process] read 701284 sequences (100000145 bp) in 2.102 CPU sec, 2.201 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290693, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.68, 122.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 67, #reads = 1091927 mem = 536881798 peak = 1096519680
[M::process] read 705380 sequences (100000108 bp) in 2.685 CPU sec, 2.864 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291470, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (241.35, 116.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697998 reads in 157.278 CPU sec, 5.018 real sec
[M::mem_process_seqs] Processed 701284 reads in 104.922 CPU sec, 3.315 real sec
[M::process] read 703196 sequences (100000109 bp) in 1.890 CPU sec, 1.971 real sec...
sorting block 68, #reads = 1089978 mem = 536879805 peak = 1096519680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293370, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (231.99, 110.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (392, 493, 951)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2069)
[M::mem_pestat] mean and std.dev: (567.33, 304.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2628)
[M::mem_pestat] skip orientation RR
[M::process] read 703150 sequences (100000166 bp) in 2.475 CPU sec, 2.611 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292502, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (237.67, 113.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 705380 reads in 144.442 CPU sec, 4.596 real sec
[M::mem_process_seqs] Processed 703196 reads in 90.366 CPU sec, 2.853 real sec
[M::process] read 702432 sequences (100000093 bp) in 1.740 CPU sec, 1.766 real sec...
sorting block 69, #reads = 1091888 mem = 536878601 peak = 1096519680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292082, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.54, 114.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701748 sequences (100000087 bp) in 2.320 CPU sec, 2.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292205, 9, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 199, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362)
[M::mem_pestat] mean and std.dev: (327.38, 257.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1769)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.33, 115.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 703150 reads in 144.639 CPU sec, 4.614 real sec
[M::process] read 701960 sequences (100000191 bp) in 1.694 CPU sec, 1.722 real sec...
[M::mem_process_seqs] Processed 702432 reads in 91.138 CPU sec, 2.877 real sec
sorting block 70, #reads = 1095446 mem = 536877599 peak = 1096519680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292571, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.58, 117.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701944 sequences (100000208 bp) in 2.729 CPU sec, 2.878 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292108, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.11, 118.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 175, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696)
[M::mem_pestat] mean and std.dev: (221.00, 88.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 881)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701748 reads in 144.287 CPU sec, 4.596 real sec
sorting block 71, #reads = 1094086 mem = 536879249 peak = 1096519680
[M::mem_process_seqs] Processed 701960 reads in 92.181 CPU sec, 2.913 real sec
[M::process] read 701100 sequences (100000003 bp) in 1.828 CPU sec, 1.884 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292296, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.04, 118.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 72, #reads = 1095714 mem = 536880668 peak = 1096519680
[M::process] read 700508 sequences (100000193 bp) in 2.645 CPU sec, 2.816 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291956, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.24, 119.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701944 reads in 147.900 CPU sec, 4.715 real sec
[M::mem_process_seqs] Processed 701100 reads in 91.715 CPU sec, 2.897 real sec
[M::process] read 698976 sequences (100000091 bp) in 1.823 CPU sec, 1.870 real sec...
sorting block 73, #reads = 1093434 mem = 536878342 peak = 1096519680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292053, 7, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (247.91, 121.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 284, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (261.83, 66.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 702)
[M::mem_pestat] skip orientation RR
[M::process] read 699048 sequences (100000097 bp) in 2.518 CPU sec, 2.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291207, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 380, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687)
[M::mem_pestat] mean and std.dev: (299.64, 103.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.94, 121.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700508 reads in 145.792 CPU sec, 4.647 real sec
[M::mem_process_seqs] Processed 698976 reads in 93.636 CPU sec, 2.956 real sec
[M::process] read 698660 sequences (100000159 bp) in 1.745 CPU sec, 1.783 real sec...
sorting block 74, #reads = 1093482 mem = 536876391 peak = 1096519680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290901, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.46, 120.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699082 sequences (100000112 bp) in 2.087 CPU sec, 2.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290586, 10, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (110, 222, 902)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2486)
[M::mem_pestat] mean and std.dev: (245.88, 254.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3278)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (249.03, 119.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (275, 361, 712)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1586)
[M::mem_pestat] mean and std.dev: (375.89, 239.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2023)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 329, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (337.77, 169.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698864 sequences (100000028 bp) in 1.430 CPU sec, 1.438 real sec...
[M::mem_process_seqs] Processed 699048 reads in 147.037 CPU sec, 4.686 real sec
sorting block 75, #reads = 1092077 mem = 536877983 peak = 1096519680
[M::mem_process_seqs] Processed 698660 reads in 91.873 CPU sec, 2.917 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291291, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 333, 1317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3599)
[M::mem_pestat] mean and std.dev: (738.00, 860.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4740)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (248.11, 119.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 76, #reads = 1092815 mem = 536878821 peak = 1096519680
[M::process] read 698482 sequences (100000267 bp) in 2.236 CPU sec, 2.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290774, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.47, 119.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 459, 1005)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2519)
[M::mem_pestat] mean and std.dev: (558.70, 379.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3276)
[M::mem_pestat] skip orientation RR
[M::process] read 698374 sequences (100000007 bp) in 1.470 CPU sec, 1.464 real sec...
[M::mem_process_seqs] Processed 699082 reads in 152.656 CPU sec, 4.866 real sec
[M::mem_process_seqs] Processed 698864 reads in 98.869 CPU sec, 3.133 real sec
sorting block 77, #reads = 1090721 mem = 536879800 peak = 1096519680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290945, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (249.10, 119.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701162 sequences (100000050 bp) in 2.475 CPU sec, 2.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290969, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.44, 119.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 708592 sequences (100000249 bp) in 1.500 CPU sec, 1.499 real sec...
[M::mem_process_seqs] Processed 698482 reads in 149.886 CPU sec, 4.764 real sec
[M::mem_process_seqs] Processed 698374 reads in 95.133 CPU sec, 3.015 real sec
sorting block 78, #reads = 1089889 mem = 536879056 peak = 1096519680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290831, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.65, 116.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 706302 sequences (100000250 bp) in 2.125 CPU sec, 2.237 real sec...
[M::process] read 705250 sequences (100000227 bp) in 1.470 CPU sec, 1.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 294159, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571)
[M::mem_pestat] mean and std.dev: (223.82, 105.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 713)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701162 reads in 161.661 CPU sec, 5.155 real sec
[M::mem_process_seqs] Processed 708592 reads in 96.656 CPU sec, 3.073 real sec
sorting block 79, #reads = 1087534 mem = 536877361 peak = 1096519680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293674, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (229.77, 109.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 80, #reads = 1089572 mem = 536879851 peak = 1096519680
[M::process] read 706638 sequences (100000109 bp) in 2.154 CPU sec, 2.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293477, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (232.91, 111.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704654 sequences (100000077 bp) in 1.470 CPU sec, 1.467 real sec...
[M::mem_process_seqs] Processed 706302 reads in 148.601 CPU sec, 4.734 real sec
[M::mem_process_seqs] Processed 705250 reads in 92.801 CPU sec, 2.942 real sec
sorting block 81, #reads = 1094134 mem = 536879045 peak = 1096519680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 294013, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (229.05, 108.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704192 sequences (100000220 bp) in 2.214 CPU sec, 2.343 real sec...
[M::process] read 702896 sequences (100000202 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293266, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (233.94, 112.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 164, 214)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 352)
[M::mem_pestat] mean and std.dev: (176.78, 67.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 446)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 706638 reads in 152.616 CPU sec, 4.860 real sec
[M::mem_process_seqs] Processed 704654 reads in 93.853 CPU sec, 2.981 real sec
sorting block 82, #reads = 1092601 mem = 536877087 peak = 1096519680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292839, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 209, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (233.50, 111.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701686 sequences (100000264 bp) in 2.324 CPU sec, 2.475 real sec...
[M::process] read 700004 sequences (100000056 bp) in 1.460 CPU sec, 1.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293104, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (239.50, 116.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704192 reads in 150.559 CPU sec, 4.790 real sec
[M::mem_process_seqs] Processed 702896 reads in 93.437 CPU sec, 2.966 real sec
sorting block 83, #reads = 1092176 mem = 536881486 peak = 1096519680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292420, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (244.30, 119.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699030 sequences (100000005 bp) in 2.252 CPU sec, 2.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291774, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 382, 1910)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5388)
[M::mem_pestat] mean and std.dev: (1014.10, 1146.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7127)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (246.18, 119.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699370 sequences (100000001 bp) in 1.440 CPU sec, 1.446 real sec...
[M::mem_process_seqs] Processed 701686 reads in 148.783 CPU sec, 4.738 real sec
sorting block 84, #reads = 1091719 mem = 536877453 peak = 1096519680
[M::mem_process_seqs] Processed 700004 reads in 93.489 CPU sec, 2.968 real sec
sorting block 85, #reads = 1092162 mem = 536880795 peak = 1096519680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290987, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.87, 119.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 256, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (241.50, 50.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RR
[M::process] read 698662 sequences (100000270 bp) in 2.202 CPU sec, 2.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291377, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.50, 118.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697686 sequences (100000107 bp) in 1.450 CPU sec, 1.442 real sec...
[M::mem_process_seqs] Processed 699030 reads in 146.993 CPU sec, 4.682 real sec
[M::mem_process_seqs] Processed 699370 reads in 91.357 CPU sec, 2.897 real sec
sorting block 86, #reads = 1090467 mem = 536878029 peak = 1096519680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291028, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.43, 119.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697536 sequences (100000278 bp) in 2.373 CPU sec, 2.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290954, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.91, 119.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698662 reads in 144.398 CPU sec, 4.594 real sec
[M::process] read 697422 sequences (100000188 bp) in 1.596 CPU sec, 1.606 real sec...
[M::mem_process_seqs] Processed 697686 reads in 90.713 CPU sec, 2.865 real sec
sorting block 87, #reads = 1092288 mem = 536878361 peak = 1096519680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290317, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 254, 1255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3341)
[M::mem_pestat] mean and std.dev: (474.33, 583.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4384)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.83, 120.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697818 sequences (100000145 bp) in 2.329 CPU sec, 2.460 real sec...
[M::process] read 50040 sequences (7148027 bp) in 0.390 CPU sec, 0.391 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290534, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.87, 121.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697536 reads in 141.910 CPU sec, 4.550 real sec
[M::mem_process_seqs] Processed 697422 reads in 88.500 CPU sec, 2.838 real sec
sorting block 88, #reads = 1092136 mem = 536880729 peak = 1096519680
sorting block 89, #reads = 1090966 mem = 536881241 peak = 1096519680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290934, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (251.09, 121.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 20822, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (245.80, 117.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697818 reads in 83.629 CPU sec, 2.735 real sec
[M::mem_process_seqs] Processed 50040 reads in 19.228 CPU sec, 0.626 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 98696224
overall: 70046633984 mem 11882.664 user 369.205 sys 1182.519 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L002\tPU:L002\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0003.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0003.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1182.570 sec; CPU: 12251.884 sec
sorting block 90, #reads = 812612 mem = 400049090 peak = 1096519680
uniq header count 1
merging 91 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5214123794
output reads: 99199403
bam_mem_sort: 91 calls 37.218 user 0.776 sys 38.201 real
bam_write: 91 calls 37.702 user 1.219 sys 39.343 real
execute: 1 calls 19.180 user 0.798 sys 1209.607 real
merge_files: 1 calls 19.147 user 0.786 sys 26.067 real
parse_chunk: 1452 calls 49.398 user 13.175 sys 63.736 real
read_chunk: 3922 calls 41.113 user 36.648 sys 353.592 real
sort_block: 1 calls 32.571 user 36.114 sys 341.585 real
write_chunk: 2468 calls 18.817 user 0.554 sys 25.260 real
overall: 3100225536 mem 1051.076 user 61.270 sys 1209.629 real
ls: cannot access '*.cram': No such file or directory