This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_Kh-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1691.932 CPU sec, 60.959 real sec
[M::process] read 671538 sequences (100000013 bp) in 0.987 CPU sec, 0.991 real sec...
[M::process] read 671154 sequences (100000046 bp) in 1.213 CPU sec, 1.250 real sec...
[M::process] read 670832 sequences (100000058 bp) in 1.147 CPU sec, 1.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278131, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 374, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (390.37, 167.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (1262, 5463, 5463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13865)
[M::mem_pestat] mean and std.dev: (3362.50, 2100.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18066)
[M::mem_pestat] skip orientation RR
[M::process] read 670536 sequences (100000017 bp) in 1.507 CPU sec, 1.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278328, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 388, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (401.28, 169.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278512, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 380, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (394.40, 166.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671538 reads in 235.804 CPU sec, 7.599 real sec
[M::mem_process_seqs] Processed 670832 reads in 162.803 CPU sec, 5.273 real sec
[M::mem_process_seqs] Processed 671154 reads in 177.618 CPU sec, 5.752 real sec
sorting block 0, #reads = 1074341 mem = 536878584 peak = 600440832
[M::process] read 670560 sequences (100000191 bp) in 2.050 CPU sec, 2.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278217, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 392, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (405.44, 170.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670376 sequences (100000122 bp) in 1.484 CPU sec, 1.489 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277376, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 393, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (405.71, 170.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670536 reads in 153.650 CPU sec, 4.852 real sec
[M::process] read 670200 sequences (100000150 bp) in 1.576 CPU sec, 1.602 real sec...
sorting block 1, #reads = 1074666 mem = 536885331 peak = 842661888
[M::mem_process_seqs] Processed 670560 reads in 99.215 CPU sec, 3.116 real sec
sorting block 2, #reads = 1075546 mem = 536879907 peak = 892612608
[M::process] read 670132 sequences (100000210 bp) in 1.888 CPU sec, 2.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277034, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 393, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (405.85, 169.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670104 sequences (100000234 bp) in 1.472 CPU sec, 1.486 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277764, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 394, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (406.64, 168.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277848, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 399, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (410.11, 170.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670376 reads in 225.781 CPU sec, 7.217 real sec
[M::mem_process_seqs] Processed 670200 reads in 167.620 CPU sec, 5.381 real sec
[M::mem_process_seqs] Processed 670132 reads in 182.441 CPU sec, 5.872 real sec
sorting block 3, #reads = 1074689 mem = 536879184 peak = 892612608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278174, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (281, 402, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (413.62, 171.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670408 sequences (100000031 bp) in 2.619 CPU sec, 2.814 real sec...
[M::mem_process_seqs] Processed 670104 reads in 89.893 CPU sec, 2.947 real sec
sorting block 4, #reads = 1074799 mem = 536877289 peak = 892612608
[M::process] read 670662 sequences (100000045 bp) in 2.450 CPU sec, 2.672 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278106, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 399, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (410.86, 172.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670560 sequences (100000095 bp) in 1.683 CPU sec, 1.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278131, 0, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 391, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (404.62, 171.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (2315, 2315, 5491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11843)
[M::mem_pestat] mean and std.dev: (3599.62, 1631.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15019)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 670408 reads in 164.538 CPU sec, 5.181 real sec
[M::process] read 670264 sequences (100000100 bp) in 1.600 CPU sec, 1.643 real sec...
[M::mem_process_seqs] Processed 670662 reads in 106.096 CPU sec, 3.330 real sec
sorting block 5, #reads = 1075877 mem = 536878602 peak = 892612608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 276896, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 393, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (405.85, 170.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670364 sequences (100000193 bp) in 2.218 CPU sec, 2.368 real sec...
[M::process] read 670408 sequences (100000174 bp) in 1.281 CPU sec, 1.291 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277460, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 394, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (406.93, 170.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670560 reads in 163.160 CPU sec, 5.194 real sec
sorting block 6, #reads = 1075773 mem = 536878355 peak = 892612608
[M::mem_process_seqs] Processed 670264 reads in 102.267 CPU sec, 3.254 real sec
sorting block 7, #reads = 1074938 mem = 536885435 peak = 892612608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277691, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 393, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (407.22, 171.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670256 sequences (100000296 bp) in 2.562 CPU sec, 2.697 real sec...
[M::process] read 670352 sequences (100000294 bp) in 1.769 CPU sec, 1.767 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277160, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 391, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (405.07, 170.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670364 reads in 165.998 CPU sec, 5.260 real sec
[M::mem_process_seqs] Processed 670408 reads in 104.863 CPU sec, 3.318 real sec
sorting block 8, #reads = 1075420 mem = 536878675 peak = 892612608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 276773, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 390, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (403.61, 169.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670422 sequences (100000233 bp) in 2.668 CPU sec, 2.816 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 277500, 2, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (162, 377, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (259.73, 107.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 386, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (400.44, 167.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 671512 sequences (100000149 bp) in 1.803 CPU sec, 1.812 real sec...
[M::mem_process_seqs] Processed 670256 reads in 164.148 CPU sec, 5.197 real sec
[M::mem_process_seqs] Processed 670352 reads in 104.160 CPU sec, 3.286 real sec
sorting block 9, #reads = 1075013 mem = 536878614 peak = 892612608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277780, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (396.76, 165.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670784 sequences (100000206 bp) in 2.449 CPU sec, 2.570 real sec...
[M::process] read 670536 sequences (100000155 bp) in 1.649 CPU sec, 1.646 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278118, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 366, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (380.03, 157.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670422 reads in 167.911 CPU sec, 5.332 real sec
sorting block 10, #reads = 1074736 mem = 536880192 peak = 892612608
[M::mem_process_seqs] Processed 671512 reads in 108.866 CPU sec, 3.456 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277170, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 375, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (390.02, 163.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670470 sequences (100000158 bp) in 2.514 CPU sec, 2.653 real sec...
[M::process] read 670602 sequences (100000059 bp) in 1.670 CPU sec, 1.674 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 276938, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (393.65, 165.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670784 reads in 169.149 CPU sec, 5.364 real sec
sorting block 11, #reads = 1072490 mem = 536881587 peak = 892612608
[M::mem_process_seqs] Processed 670536 reads in 105.988 CPU sec, 3.358 real sec
sorting block 12, #reads = 1074549 mem = 536876781 peak = 892612608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 276447, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 378, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (395.54, 167.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670356 sequences (100000033 bp) in 2.567 CPU sec, 2.701 real sec...
[M::process] read 670360 sequences (100000131 bp) in 1.800 CPU sec, 1.804 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277444, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (394.64, 166.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670470 reads in 170.279 CPU sec, 5.397 real sec
[M::mem_process_seqs] Processed 670602 reads in 104.614 CPU sec, 3.311 real sec
sorting block 13, #reads = 1074836 mem = 536879042 peak = 892612608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277090, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (396.18, 166.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670244 sequences (100000058 bp) in 2.522 CPU sec, 2.650 real sec...
[M::process] read 670380 sequences (100000103 bp) in 1.350 CPU sec, 1.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 277350, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (397.68, 167.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670356 reads in 160.970 CPU sec, 5.112 real sec
[M::mem_process_seqs] Processed 670360 reads in 100.553 CPU sec, 3.192 real sec
sorting block 14, #reads = 1075660 mem = 536878568 peak = 892612608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 277336, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (397.75, 167.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670070 sequences (100000227 bp) in 2.594 CPU sec, 2.737 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277888, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (397.61, 167.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670324 sequences (100000144 bp) in 1.750 CPU sec, 1.745 real sec...
[M::mem_process_seqs] Processed 670244 reads in 160.863 CPU sec, 5.100 real sec
[M::mem_process_seqs] Processed 670380 reads in 100.959 CPU sec, 3.189 real sec
sorting block 15, #reads = 1074875 mem = 536881316 peak = 892612608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277743, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 384, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (400.64, 168.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670922 sequences (100000264 bp) in 2.680 CPU sec, 2.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278085, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (398.43, 168.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670642 sequences (100000037 bp) in 1.729 CPU sec, 1.730 real sec...
[M::mem_process_seqs] Processed 670070 reads in 156.721 CPU sec, 4.965 real sec
sorting block 16, #reads = 1075277 mem = 536880663 peak = 892612608
[M::mem_process_seqs] Processed 670324 reads in 98.550 CPU sec, 3.103 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278161, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (394.93, 168.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 17, #reads = 1074649 mem = 536879445 peak = 921821184
[M::process] read 670682 sequences (100000242 bp) in 2.626 CPU sec, 2.766 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278151, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 386, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (400.00, 169.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670296 sequences (100000120 bp) in 1.459 CPU sec, 1.460 real sec...
[M::mem_process_seqs] Processed 670922 reads in 147.483 CPU sec, 4.681 real sec
[M::mem_process_seqs] Processed 670642 reads in 93.750 CPU sec, 2.961 real sec
sorting block 18, #reads = 1076783 mem = 536882665 peak = 921821184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 276896, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 389, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (403.88, 171.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670230 sequences (100000182 bp) in 2.787 CPU sec, 2.942 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277821, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 397, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (410.44, 173.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670682 reads in 144.322 CPU sec, 4.574 real sec
[M::mem_process_seqs] Processed 670296 reads in 92.228 CPU sec, 2.904 real sec
[M::process] read 670020 sequences (100000228 bp) in 2.110 CPU sec, 2.185 real sec...
sorting block 19, #reads = 1076920 mem = 536878745 peak = 921821184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277677, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 401, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (413.84, 175.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669988 sequences (100000038 bp) in 2.230 CPU sec, 2.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278086, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 400, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (412.51, 173.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (652, 652, 5436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15004)
[M::mem_pestat] mean and std.dev: (2565.60, 2343.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 19788)
[M::mem_pestat] skip orientation RR
[M::process] read 669822 sequences (100000133 bp) in 1.340 CPU sec, 1.334 real sec...
[M::mem_process_seqs] Processed 670230 reads in 148.809 CPU sec, 4.734 real sec
[M::mem_process_seqs] Processed 670020 reads in 94.846 CPU sec, 2.998 real sec
sorting block 20, #reads = 1076522 mem = 536881315 peak = 921821184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 277653, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (279, 400, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (412.56, 173.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669698 sequences (100000299 bp) in 2.671 CPU sec, 2.838 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 276921, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (283, 405, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (416.86, 174.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669988 reads in 146.339 CPU sec, 4.637 real sec
[M::process] read 669672 sequences (100000045 bp) in 1.697 CPU sec, 1.718 real sec...
sorting block 21, #reads = 1076599 mem = 536876873 peak = 921821184
[M::mem_process_seqs] Processed 669822 reads in 93.172 CPU sec, 2.935 real sec
sorting block 22, #reads = 1075897 mem = 536878922 peak = 931266560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 276435, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (283, 406, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (418.29, 175.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670388 sequences (100000187 bp) in 2.700 CPU sec, 2.857 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 276418, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (284, 406, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (418.50, 174.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669698 reads in 152.274 CPU sec, 4.830 real sec
[M::process] read 670254 sequences (100000120 bp) in 1.777 CPU sec, 1.788 real sec...
[M::mem_process_seqs] Processed 669672 reads in 95.183 CPU sec, 2.993 real sec
sorting block 23, #reads = 1074734 mem = 536879682 peak = 931266560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277545, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 397, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (410.39, 174.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670312 sequences (100000030 bp) in 2.661 CPU sec, 2.818 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277796, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 395, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (409.62, 173.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670388 reads in 156.997 CPU sec, 4.983 real sec
[M::process] read 670208 sequences (100000273 bp) in 1.857 CPU sec, 1.880 real sec...
[M::mem_process_seqs] Processed 670254 reads in 101.778 CPU sec, 3.207 real sec
sorting block 24, #reads = 1075051 mem = 536878469 peak = 931266560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277796, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 395, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (408.93, 172.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670196 sequences (100000215 bp) in 2.538 CPU sec, 2.720 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277901, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 393, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (407.20, 172.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670368 sequences (100000013 bp) in 1.801 CPU sec, 1.807 real sec...
[M::mem_process_seqs] Processed 670312 reads in 163.589 CPU sec, 5.189 real sec
sorting block 25, #reads = 1075991 mem = 536878008 peak = 931266560
[M::mem_process_seqs] Processed 670208 reads in 104.374 CPU sec, 3.288 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277998, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 390, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (405.25, 171.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670536 sequences (100000183 bp) in 2.760 CPU sec, 2.919 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 277652, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 389, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (404.81, 171.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670196 reads in 157.570 CPU sec, 4.996 real sec
[M::process] read 670602 sequences (100000251 bp) in 1.864 CPU sec, 1.867 real sec...
sorting block 26, #reads = 1076182 mem = 536879976 peak = 931266560
[M::mem_process_seqs] Processed 670368 reads in 99.574 CPU sec, 3.139 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277197, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 385, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (400.24, 169.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 27, #reads = 1076301 mem = 536878862 peak = 994672640
[M::process] read 670830 sequences (100000207 bp) in 2.684 CPU sec, 2.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277177, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 383, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (398.95, 169.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671736 sequences (100000276 bp) in 1.570 CPU sec, 1.574 real sec...
[M::mem_process_seqs] Processed 670536 reads in 160.050 CPU sec, 5.085 real sec
[M::mem_process_seqs] Processed 670602 reads in 103.586 CPU sec, 3.277 real sec
sorting block 28, #reads = 1074499 mem = 536877413 peak = 994672640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277934, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (398.29, 170.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670632 sequences (100000106 bp) in 2.627 CPU sec, 2.802 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278685, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 373, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (387.78, 164.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670548 sequences (100000151 bp) in 1.737 CPU sec, 1.743 real sec...
[M::mem_process_seqs] Processed 670830 reads in 162.727 CPU sec, 5.158 real sec
[M::mem_process_seqs] Processed 671736 reads in 108.354 CPU sec, 3.423 real sec
sorting block 29, #reads = 1074956 mem = 536878633 peak = 994672640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277934, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 384, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (399.96, 170.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670558 sequences (100000182 bp) in 2.453 CPU sec, 2.605 real sec...
[M::process] read 670388 sequences (100000112 bp) in 1.358 CPU sec, 1.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277935, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 384, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (400.99, 170.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670632 reads in 154.290 CPU sec, 4.902 real sec
sorting block 30, #reads = 1072334 mem = 536882688 peak = 994672640
[M::mem_process_seqs] Processed 670548 reads in 98.339 CPU sec, 3.113 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277581, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 382, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (398.83, 170.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670234 sequences (100000079 bp) in 2.769 CPU sec, 2.903 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277948, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 383, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (399.67, 169.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670186 sequences (100000080 bp) in 1.658 CPU sec, 1.664 real sec...
[M::mem_process_seqs] Processed 670558 reads in 153.792 CPU sec, 4.871 real sec
sorting block 31, #reads = 1075846 mem = 536881416 peak = 994672640
[M::mem_process_seqs] Processed 670388 reads in 97.729 CPU sec, 3.084 real sec
sorting block 32, #reads = 1074823 mem = 536878256 peak = 994672640
[M::process] read 670134 sequences (100000165 bp) in 2.582 CPU sec, 2.724 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278586, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 383, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (400.29, 169.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670186 reads in 84.162 CPU sec, 2.643 real sec
[M::process] read 670064 sequences (100000135 bp) in 2.274 CPU sec, 2.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277792, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 381, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (397.77, 168.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278337, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 386, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (402.11, 170.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670234 reads in 236.670 CPU sec, 7.502 real sec
[M::mem_process_seqs] Processed 670134 reads in 114.138 CPU sec, 3.603 real sec
sorting block 33, #reads = 1075951 mem = 536879012 peak = 994672640
[M::process] read 669978 sequences (100000076 bp) in 1.992 CPU sec, 2.054 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278524, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 390, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (406.27, 172.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 34, #reads = 1074722 mem = 536878284 peak = 994672640
[M::process] read 671178 sequences (100000113 bp) in 2.664 CPU sec, 2.849 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278596, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 392, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (409.10, 174.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670064 reads in 150.719 CPU sec, 4.790 real sec
[M::mem_process_seqs] Processed 669978 reads in 99.531 CPU sec, 3.137 real sec
[M::process] read 670760 sequences (100000170 bp) in 2.121 CPU sec, 2.214 real sec...
sorting block 35, #reads = 1075458 mem = 536879525 peak = 994672640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278506, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 382, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (397.82, 171.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670412 sequences (100000239 bp) in 2.446 CPU sec, 2.609 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278477, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 388, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (402.33, 171.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671178 reads in 151.936 CPU sec, 4.824 real sec
[M::process] read 670370 sequences (100000065 bp) in 1.790 CPU sec, 1.814 real sec...
[M::mem_process_seqs] Processed 670760 reads in 99.208 CPU sec, 3.120 real sec
sorting block 36, #reads = 1074584 mem = 536877908 peak = 994672640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279049, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 392, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (406.68, 172.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670026 sequences (100000045 bp) in 2.307 CPU sec, 2.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278694, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 393, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (407.34, 172.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670412 reads in 146.164 CPU sec, 4.638 real sec
[M::process] read 669994 sequences (100000018 bp) in 1.892 CPU sec, 1.921 real sec...
[M::mem_process_seqs] Processed 670370 reads in 94.169 CPU sec, 2.961 real sec
sorting block 37, #reads = 1074696 mem = 536883420 peak = 994672640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278530, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (279, 398, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (412.16, 173.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669798 sequences (100000057 bp) in 2.520 CPU sec, 2.662 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 278251, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (281, 402, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (414.97, 174.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670026 reads in 146.584 CPU sec, 4.646 real sec
[M::process] read 669656 sequences (100000201 bp) in 1.940 CPU sec, 1.973 real sec...
sorting block 38, #reads = 1075237 mem = 536878014 peak = 994672640
[M::mem_process_seqs] Processed 669994 reads in 95.130 CPU sec, 2.991 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278310, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 404, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (416.32, 174.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 39, #reads = 1075855 mem = 536878092 peak = 994672640
[M::process] read 669498 sequences (100000075 bp) in 2.353 CPU sec, 2.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278244, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (284, 407, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (419.81, 176.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669562 sequences (100000279 bp) in 1.370 CPU sec, 1.370 real sec...
[M::mem_process_seqs] Processed 669798 reads in 146.449 CPU sec, 4.654 real sec
[M::mem_process_seqs] Processed 669656 reads in 94.219 CPU sec, 2.970 real sec
sorting block 40, #reads = 1075674 mem = 536880812 peak = 994672640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278196, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (288, 413, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062)
[M::mem_pestat] mean and std.dev: (424.61, 177.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1320)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670090 sequences (100000163 bp) in 2.370 CPU sec, 2.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279019, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (288, 414, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068)
[M::mem_pestat] mean and std.dev: (425.61, 178.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1328)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670516 sequences (100000262 bp) in 1.560 CPU sec, 1.562 real sec...
[M::mem_process_seqs] Processed 669498 reads in 147.286 CPU sec, 4.671 real sec
[M::mem_process_seqs] Processed 669562 reads in 96.855 CPU sec, 3.061 real sec
sorting block 41, #reads = 1075412 mem = 536881631 peak = 994672640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278095, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 400, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (413.96, 176.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670048 sequences (100000148 bp) in 2.667 CPU sec, 2.829 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278303, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 392, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (407.65, 174.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670090 reads in 152.913 CPU sec, 4.838 real sec
[M::process] read 670046 sequences (100000084 bp) in 1.933 CPU sec, 1.958 real sec...
[M::mem_process_seqs] Processed 670516 reads in 97.773 CPU sec, 3.076 real sec
sorting block 42, #reads = 1075663 mem = 536881551 peak = 994672640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278392, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 396, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (410.82, 174.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670242 sequences (100000152 bp) in 2.627 CPU sec, 2.804 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278163, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 396, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (410.89, 175.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670048 reads in 151.234 CPU sec, 4.789 real sec
[M::process] read 670314 sequences (100000073 bp) in 2.021 CPU sec, 2.046 real sec...
sorting block 43, #reads = 1075643 mem = 536880138 peak = 994672640
[M::mem_process_seqs] Processed 670046 reads in 97.761 CPU sec, 3.070 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278229, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 393, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (408.37, 174.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 44, #reads = 1075375 mem = 536880147 peak = 994672640
[M::process] read 670440 sequences (100000063 bp) in 2.605 CPU sec, 2.759 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278030, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 394, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (408.94, 175.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670242 reads in 154.124 CPU sec, 4.893 real sec
[M::process] read 670284 sequences (100000279 bp) in 1.920 CPU sec, 1.936 real sec...
[M::mem_process_seqs] Processed 670314 reads in 100.224 CPU sec, 3.148 real sec
sorting block 45, #reads = 1075558 mem = 536882427 peak = 994672640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 277364, 2, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (5528, 5634, 5634)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5316, 5846)
[M::mem_pestat] mean and std.dev: (5608.38, 46.46)
[M::mem_pestat] low and high boundaries for proper pairs: (5210, 5952)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 389, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (405.09, 174.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 670504 sequences (100000269 bp) in 2.451 CPU sec, 2.614 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 278114, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 388, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (405.07, 174.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670440 reads in 152.452 CPU sec, 4.834 real sec
[M::process] read 670736 sequences (100000261 bp) in 1.740 CPU sec, 1.742 real sec...
[M::mem_process_seqs] Processed 670284 reads in 98.511 CPU sec, 3.111 real sec
sorting block 46, #reads = 1075419 mem = 536877277 peak = 994672640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278125, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 384, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (400.82, 171.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670348 sequences (100000040 bp) in 2.728 CPU sec, 2.895 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 278441, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (397.88, 170.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (293, 851, 5475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15839)
[M::mem_pestat] mean and std.dev: (2008.67, 2200.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 21021)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 670504 reads in 156.183 CPU sec, 4.944 real sec
[M::process] read 670352 sequences (100000010 bp) in 1.937 CPU sec, 1.946 real sec...
[M::mem_process_seqs] Processed 670736 reads in 100.854 CPU sec, 3.175 real sec
sorting block 47, #reads = 1075379 mem = 536880657 peak = 994672640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278815, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 388, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (405.27, 174.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670142 sequences (100000176 bp) in 2.635 CPU sec, 2.789 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277583, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 389, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (406.57, 175.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670372 sequences (100000269 bp) in 1.670 CPU sec, 1.670 real sec...
[M::mem_process_seqs] Processed 670348 reads in 156.386 CPU sec, 4.955 real sec
sorting block 48, #reads = 1075167 mem = 536879838 peak = 994672640
[M::mem_process_seqs] Processed 670352 reads in 100.282 CPU sec, 3.164 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277617, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 390, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (408.29, 176.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1076130 mem = 536880759 peak = 994672640
[M::process] read 670488 sequences (100000000 bp) in 2.431 CPU sec, 2.583 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277832, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 387, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (405.47, 175.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670142 reads in 152.840 CPU sec, 4.849 real sec
[M::process] read 670292 sequences (100000210 bp) in 1.995 CPU sec, 2.025 real sec...
[M::mem_process_seqs] Processed 670372 reads in 96.821 CPU sec, 3.045 real sec
sorting block 50, #reads = 1076469 mem = 536877718 peak = 994672640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278435, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 389, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (406.41, 175.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670204 sequences (100000175 bp) in 2.545 CPU sec, 2.713 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277849, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 389, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (407.01, 175.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670488 reads in 151.475 CPU sec, 4.805 real sec
[M::mem_process_seqs] Processed 670292 reads in 98.659 CPU sec, 3.094 real sec
[M::process] read 670178 sequences (100000037 bp) in 2.046 CPU sec, 2.088 real sec...
sorting block 51, #reads = 1076184 mem = 536877699 peak = 994672640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278174, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 390, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (407.98, 175.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669992 sequences (100000030 bp) in 2.554 CPU sec, 2.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278704, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 391, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (408.33, 176.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670204 reads in 151.455 CPU sec, 4.804 real sec
[M::mem_process_seqs] Processed 670178 reads in 97.708 CPU sec, 3.067 real sec
[M::process] read 670330 sequences (100000162 bp) in 2.109 CPU sec, 2.159 real sec...
sorting block 52, #reads = 1075127 mem = 536879897 peak = 994672640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278119, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 394, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (412.28, 177.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670746 sequences (100000104 bp) in 2.395 CPU sec, 2.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278079, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 387, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (404.27, 173.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670388 sequences (100000161 bp) in 1.450 CPU sec, 1.446 real sec...
[M::mem_process_seqs] Processed 669992 reads in 154.201 CPU sec, 4.908 real sec
sorting block 53, #reads = 1075169 mem = 536879544 peak = 994672640
[M::mem_process_seqs] Processed 670330 reads in 101.357 CPU sec, 3.214 real sec
sorting block 54, #reads = 1074771 mem = 536879916 peak = 1021194240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277926, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 387, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (402.67, 172.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670212 sequences (100000257 bp) in 2.725 CPU sec, 2.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278302, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 393, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (407.94, 173.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (5808, 6579, 6579)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4266, 8121)
[M::mem_pestat] mean and std.dev: (6322.00, 363.45)
[M::mem_pestat] low and high boundaries for proper pairs: (3495, 8892)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 670746 reads in 154.769 CPU sec, 4.898 real sec
[M::process] read 670014 sequences (100000010 bp) in 1.905 CPU sec, 1.922 real sec...
[M::mem_process_seqs] Processed 670388 reads in 100.422 CPU sec, 3.159 real sec
sorting block 55, #reads = 1076090 mem = 536881981 peak = 1021194240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 277748, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 396, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (411.18, 175.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670062 sequences (100000018 bp) in 2.604 CPU sec, 2.773 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277642, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 400, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049)
[M::mem_pestat] mean and std.dev: (414.35, 176.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670212 reads in 153.366 CPU sec, 4.865 real sec
[M::process] read 669992 sequences (100000294 bp) in 1.841 CPU sec, 1.860 real sec...
[M::mem_process_seqs] Processed 670014 reads in 99.306 CPU sec, 3.131 real sec
sorting block 56, #reads = 1076624 mem = 536879143 peak = 1021194240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277041, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (280, 403, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (417.03, 177.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669766 sequences (100000138 bp) in 2.572 CPU sec, 2.740 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277428, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (280, 405, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (417.75, 177.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1312)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670062 reads in 152.688 CPU sec, 4.844 real sec
[M::process] read 669464 sequences (100000144 bp) in 1.733 CPU sec, 1.735 real sec...
[M::mem_process_seqs] Processed 669992 reads in 96.383 CPU sec, 3.039 real sec
sorting block 57, #reads = 1076050 mem = 536878076 peak = 1021194240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 276982, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (283, 406, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (419.87, 176.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1311)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669426 sequences (100000069 bp) in 2.638 CPU sec, 2.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277473, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (285, 408, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056)
[M::mem_pestat] mean and std.dev: (421.30, 176.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669304 sequences (100000010 bp) in 1.554 CPU sec, 1.558 real sec...
[M::mem_process_seqs] Processed 669766 reads in 155.125 CPU sec, 4.921 real sec
sorting block 58, #reads = 1075702 mem = 536877077 peak = 1021194240
[M::mem_process_seqs] Processed 669464 reads in 99.737 CPU sec, 3.151 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277131, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (287, 412, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064)
[M::mem_pestat] mean and std.dev: (424.24, 177.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1323)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 59, #reads = 1076630 mem = 536880152 peak = 1021194240
[M::process] read 670120 sequences (100000259 bp) in 2.696 CPU sec, 2.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 277214, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (290, 416, 551)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073)
[M::mem_pestat] mean and std.dev: (427.73, 178.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1334)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669426 reads in 155.128 CPU sec, 4.926 real sec
[M::mem_process_seqs] Processed 669304 reads in 101.149 CPU sec, 3.181 real sec
[M::process] read 670354 sequences (100000209 bp) in 2.166 CPU sec, 2.230 real sec...
sorting block 60, #reads = 1075800 mem = 536877761 peak = 1021194240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277646, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 399, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (413.73, 176.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1312)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670018 sequences (100000013 bp) in 2.338 CPU sec, 2.513 real sec...
[M::process] read 670144 sequences (100000112 bp) in 1.400 CPU sec, 1.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277505, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 397, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (412.31, 176.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1314)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670120 reads in 160.863 CPU sec, 5.129 real sec
[M::mem_process_seqs] Processed 670354 reads in 103.108 CPU sec, 3.277 real sec
sorting block 61, #reads = 1076617 mem = 536882633 peak = 1021194240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 277123, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 400, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (414.38, 176.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670314 sequences (100000270 bp) in 2.747 CPU sec, 2.913 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277129, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 395, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (409.78, 174.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670018 reads in 160.340 CPU sec, 5.085 real sec
[M::process] read 670082 sequences (100000243 bp) in 1.975 CPU sec, 1.995 real sec...
[M::mem_process_seqs] Processed 670144 reads in 104.580 CPU sec, 3.287 real sec
sorting block 62, #reads = 1076049 mem = 536878335 peak = 1021194240
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277683, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 393, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (408.18, 173.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670286 sequences (100000199 bp) in 2.768 CPU sec, 2.932 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277084, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 391, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (406.71, 172.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670314 reads in 161.769 CPU sec, 5.129 real sec
[M::process] read 670286 sequences (100000167 bp) in 1.884 CPU sec, 1.885 real sec...
sorting block 63, #reads = 1074875 mem = 536878006 peak = 1021194240
[M::mem_process_seqs] Processed 670082 reads in 100.980 CPU sec, 3.179 real sec
sorting block 64, #reads = 1074841 mem = 536878339 peak = 1053016064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278039, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 388, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (403.81, 171.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670398 sequences (100000083 bp) in 2.612 CPU sec, 2.734 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277214, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 387, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (402.16, 170.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670224 sequences (100000253 bp) in 1.741 CPU sec, 1.745 real sec...
[M::mem_process_seqs] Processed 670286 reads in 158.215 CPU sec, 5.013 real sec
[M::mem_process_seqs] Processed 670286 reads in 100.273 CPU sec, 3.162 real sec
sorting block 65, #reads = 1075341 mem = 536878292 peak = 1053016064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277492, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 387, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (402.39, 170.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670304 sequences (100000258 bp) in 2.925 CPU sec, 3.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277475, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 387, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (403.29, 170.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670398 reads in 159.289 CPU sec, 5.035 real sec
[M::process] read 670386 sequences (100000263 bp) in 1.925 CPU sec, 1.929 real sec...
[M::mem_process_seqs] Processed 670224 reads in 101.230 CPU sec, 3.185 real sec
sorting block 66, #reads = 1075043 mem = 536880518 peak = 1053016064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277735, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 390, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (404.82, 171.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670284 sequences (100000297 bp) in 2.705 CPU sec, 2.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277467, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 390, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (406.52, 173.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670348 sequences (100000112 bp) in 1.843 CPU sec, 1.851 real sec...
[M::mem_process_seqs] Processed 670304 reads in 163.601 CPU sec, 5.178 real sec
[M::mem_process_seqs] Processed 670386 reads in 105.120 CPU sec, 3.314 real sec
sorting block 67, #reads = 1075295 mem = 536887736 peak = 1053016064
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277161, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 389, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (405.87, 174.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670258 sequences (100000284 bp) in 2.602 CPU sec, 2.758 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277833, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 386, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (403.10, 171.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670044 sequences (100000125 bp) in 1.750 CPU sec, 1.750 real sec...
[M::mem_process_seqs] Processed 670284 reads in 163.592 CPU sec, 5.184 real sec
sorting block 68, #reads = 1075787 mem = 536881130 peak = 1053016064
[M::mem_process_seqs] Processed 670348 reads in 103.734 CPU sec, 3.269 real sec
sorting block 69, #reads = 1074792 mem = 536878792 peak = 1088233472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277239, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 388, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (403.72, 171.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669786 sequences (100000169 bp) in 2.781 CPU sec, 2.936 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278041, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 389, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (405.46, 171.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669822 sequences (100000297 bp) in 1.765 CPU sec, 1.770 real sec...
[M::mem_process_seqs] Processed 670258 reads in 157.861 CPU sec, 4.990 real sec
[M::mem_process_seqs] Processed 670044 reads in 102.249 CPU sec, 3.220 real sec
sorting block 70, #reads = 1074849 mem = 536879553 peak = 1088233472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277948, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 392, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (407.52, 171.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 669936 sequences (100000138 bp) in 2.742 CPU sec, 2.880 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277752, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 393, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (408.77, 173.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669786 reads in 152.404 CPU sec, 4.832 real sec
[M::mem_process_seqs] Processed 669822 reads in 98.424 CPU sec, 3.089 real sec
[M::process] read 670724 sequences (100000294 bp) in 2.454 CPU sec, 2.521 real sec...
sorting block 71, #reads = 1074839 mem = 536880909 peak = 1088233472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277381, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 395, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (410.77, 174.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672838 sequences (100000146 bp) in 2.747 CPU sec, 2.894 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278538, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 388, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (404.30, 173.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 669936 reads in 156.541 CPU sec, 4.978 real sec
[M::mem_process_seqs] Processed 670724 reads in 103.601 CPU sec, 3.262 real sec
[M::process] read 672474 sequences (100000024 bp) in 1.985 CPU sec, 2.051 real sec...
sorting block 72, #reads = 1075138 mem = 536879613 peak = 1088233472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279358, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 370, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (386.07, 170.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672224 sequences (100000017 bp) in 2.762 CPU sec, 2.905 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278331, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 376, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (391.85, 171.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (4139, 5132, 5132)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2153, 7118)
[M::mem_pestat] mean and std.dev: (4904.67, 409.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1160, 8111)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 672170 sequences (100000258 bp) in 1.720 CPU sec, 1.726 real sec...
[M::mem_process_seqs] Processed 672838 reads in 170.472 CPU sec, 5.403 real sec
sorting block 73, #reads = 1074068 mem = 536880106 peak = 1088233472
[M::mem_process_seqs] Processed 672474 reads in 110.335 CPU sec, 3.478 real sec
sorting block 74, #reads = 1072280 mem = 536881848 peak = 1088233472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278573, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 378, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (391.21, 169.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671718 sequences (100000080 bp) in 2.911 CPU sec, 3.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278786, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 377, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (389.38, 167.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671224 sequences (100000289 bp) in 1.914 CPU sec, 1.919 real sec...
[M::mem_process_seqs] Processed 672224 reads in 172.001 CPU sec, 5.442 real sec
[M::mem_process_seqs] Processed 672170 reads in 110.052 CPU sec, 3.466 real sec
sorting block 75, #reads = 1070357 mem = 536878751 peak = 1088233472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279195, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 384, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (395.01, 168.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671228 sequences (100000259 bp) in 2.459 CPU sec, 2.585 real sec...
[M::process] read 671348 sequences (100000074 bp) in 1.630 CPU sec, 1.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 278309, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 387, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (397.93, 167.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671718 reads in 171.215 CPU sec, 5.440 real sec
[M::mem_process_seqs] Processed 671224 reads in 108.226 CPU sec, 3.426 real sec
sorting block 76, #reads = 1069733 mem = 536877322 peak = 1088233472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278518, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 390, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (400.29, 168.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672432 sequences (100000117 bp) in 2.672 CPU sec, 2.809 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 278459, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 386, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (395.99, 166.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672696 sequences (100000173 bp) in 1.870 CPU sec, 1.869 real sec...
[M::mem_process_seqs] Processed 671228 reads in 166.967 CPU sec, 5.288 real sec
sorting block 77, #reads = 1070881 mem = 536879458 peak = 1088233472
[M::mem_process_seqs] Processed 671348 reads in 106.099 CPU sec, 3.347 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279461, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 372, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (385.91, 165.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672694 sequences (100000269 bp) in 2.821 CPU sec, 2.952 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279067, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 368, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (382.23, 164.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672584 sequences (100000108 bp) in 1.940 CPU sec, 1.940 real sec...
[M::mem_process_seqs] Processed 672432 reads in 176.074 CPU sec, 5.575 real sec
sorting block 78, #reads = 1072065 mem = 536884139 peak = 1088233472
[M::mem_process_seqs] Processed 672696 reads in 114.323 CPU sec, 3.612 real sec
sorting block 79, #reads = 1070780 mem = 536878413 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279195, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 371, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (384.75, 165.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672092 sequences (100000212 bp) in 3.064 CPU sec, 3.199 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279455, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 369, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (382.51, 163.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671532 sequences (100000195 bp) in 2.038 CPU sec, 2.044 real sec...
[M::mem_process_seqs] Processed 672694 reads in 185.220 CPU sec, 5.864 real sec
[M::mem_process_seqs] Processed 672584 reads in 117.702 CPU sec, 3.711 real sec
sorting block 80, #reads = 1069004 mem = 536879381 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279171, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 375, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (388.06, 165.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671518 sequences (100000146 bp) in 2.992 CPU sec, 3.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278221, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (391.10, 165.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673450 sequences (100000163 bp) in 2.040 CPU sec, 2.037 real sec...
[M::mem_process_seqs] Processed 672092 reads in 177.796 CPU sec, 5.624 real sec
[M::mem_process_seqs] Processed 671532 reads in 112.958 CPU sec, 3.563 real sec
sorting block 81, #reads = 1068587 mem = 536877561 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278378, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 379, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (392.55, 166.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672256 sequences (100000192 bp) in 2.985 CPU sec, 3.114 real sec...
[M::process] read 671970 sequences (100000209 bp) in 2.010 CPU sec, 2.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279436, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 356, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (369.16, 156.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671518 reads in 185.007 CPU sec, 5.855 real sec
sorting block 82, #reads = 1070982 mem = 536882108 peak = 1102647296
[M::mem_process_seqs] Processed 673450 reads in 125.288 CPU sec, 3.961 real sec
sorting block 83, #reads = 1065651 mem = 536880918 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278835, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 370, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (382.06, 161.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671558 sequences (100000083 bp) in 2.805 CPU sec, 2.922 real sec...
[M::process] read 671566 sequences (100000018 bp) in 1.689 CPU sec, 1.687 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278946, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 372, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (384.13, 161.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672256 reads in 187.812 CPU sec, 5.961 real sec
[M::mem_process_seqs] Processed 671970 reads in 120.858 CPU sec, 3.836 real sec
sorting block 84, #reads = 1065129 mem = 536882347 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279057, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (389.90, 164.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671374 sequences (100000119 bp) in 2.640 CPU sec, 2.777 real sec...
[M::process] read 671694 sequences (100000030 bp) in 1.973 CPU sec, 1.975 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278721, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (390.22, 164.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671558 reads in 179.121 CPU sec, 5.671 real sec
[M::mem_process_seqs] Processed 671566 reads in 114.904 CPU sec, 3.638 real sec
sorting block 85, #reads = 1068031 mem = 536880371 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278656, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (394.62, 167.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (3024, 4527, 5604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10764)
[M::mem_pestat] mean and std.dev: (4013.30, 1686.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13344)
[M::mem_pestat] skip orientation RR
[M::process] read 671280 sequences (100000148 bp) in 2.739 CPU sec, 2.868 real sec...
[M::process] read 674030 sequences (100000126 bp) in 1.720 CPU sec, 1.722 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279637, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 375, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (390.24, 166.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671374 reads in 181.837 CPU sec, 5.769 real sec
sorting block 86, #reads = 1068722 mem = 536877985 peak = 1102647296
[M::process] read 674410 sequences (100000069 bp) in 2.263 CPU sec, 2.271 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278900, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 381, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (396.37, 170.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671280 reads in 106.543 CPU sec, 3.602 real sec
[M::mem_process_seqs] Processed 671694 reads in 247.950 CPU sec, 8.097 real sec
sorting block 87, #reads = 1068123 mem = 536877322 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279993, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 354, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (372.91, 165.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673066 sequences (100000124 bp) in 2.533 CPU sec, 2.659 real sec...
[M::process] read 673020 sequences (100000252 bp) in 1.578 CPU sec, 1.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280005, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (366.09, 160.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674030 reads in 205.027 CPU sec, 6.526 real sec
[M::mem_process_seqs] Processed 674410 reads in 120.817 CPU sec, 3.831 real sec
sorting block 88, #reads = 1065782 mem = 536877737 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279704, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 363, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (377.48, 163.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672234 sequences (100000170 bp) in 2.525 CPU sec, 2.715 real sec...
[M::process] read 672160 sequences (100000071 bp) in 1.840 CPU sec, 1.840 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 279456, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (271, 853, 5552)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16114)
[M::mem_pestat] mean and std.dev: (2765.07, 2568.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 21395)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 364, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (377.65, 162.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (5442, 5442, 5442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5442, 5442)
[M::mem_pestat] mean and std.dev: (5442.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (5442, 5442)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 673066 reads in 180.992 CPU sec, 5.742 real sec
sorting block 89, #reads = 1064972 mem = 536885240 peak = 1102647296
[M::mem_process_seqs] Processed 673020 reads in 115.704 CPU sec, 3.661 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279643, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 373, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (384.16, 162.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671564 sequences (100000130 bp) in 2.622 CPU sec, 2.746 real sec...
[M::process] read 672112 sequences (100000098 bp) in 1.990 CPU sec, 1.984 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279155, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 374, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (385.31, 162.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672234 reads in 176.989 CPU sec, 5.607 real sec
sorting block 90, #reads = 1066962 mem = 536882970 peak = 1102647296
[M::mem_process_seqs] Processed 672160 reads in 113.517 CPU sec, 3.594 real sec
sorting block 91, #reads = 1066626 mem = 536878613 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279363, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 380, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (391.19, 164.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672288 sequences (100000045 bp) in 2.568 CPU sec, 2.708 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279044, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 373, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (386.74, 165.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672354 sequences (100000283 bp) in 1.990 CPU sec, 1.992 real sec...
[M::mem_process_seqs] Processed 671564 reads in 172.078 CPU sec, 5.452 real sec
[M::mem_process_seqs] Processed 672112 reads in 110.431 CPU sec, 3.479 real sec
sorting block 92, #reads = 1069114 mem = 536880954 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279538, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 370, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (384.53, 165.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672224 sequences (100000120 bp) in 2.852 CPU sec, 2.972 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279324, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 370, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (384.64, 166.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672138 sequences (100000088 bp) in 2.090 CPU sec, 2.086 real sec...
[M::mem_process_seqs] Processed 672288 reads in 170.715 CPU sec, 5.398 real sec
[M::mem_process_seqs] Processed 672354 reads in 109.556 CPU sec, 3.448 real sec
sorting block 93, #reads = 1070706 mem = 536879042 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278966, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 374, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (387.79, 166.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672238 sequences (100000015 bp) in 2.651 CPU sec, 2.787 real sec...
[M::process] read 671858 sequences (100000190 bp) in 1.680 CPU sec, 1.679 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278125, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 374, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (387.61, 166.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672224 reads in 172.037 CPU sec, 5.457 real sec
sorting block 94, #reads = 1070763 mem = 536878132 peak = 1102647296
[M::mem_process_seqs] Processed 672138 reads in 111.887 CPU sec, 3.534 real sec
sorting block 95, #reads = 1069200 mem = 536883436 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279621, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 372, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (385.49, 164.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673472 sequences (100000287 bp) in 2.450 CPU sec, 2.565 real sec...
[M::process] read 671056 sequences (100000106 bp) in 1.580 CPU sec, 1.581 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279838, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 377, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (390.70, 166.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672238 reads in 180.367 CPU sec, 5.737 real sec
[M::mem_process_seqs] Processed 671858 reads in 113.729 CPU sec, 3.615 real sec
sorting block 96, #reads = 1069836 mem = 536880280 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280422, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 358, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (373.73, 162.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670924 sequences (100000184 bp) in 2.511 CPU sec, 2.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278509, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 387, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (401.70, 172.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670888 sequences (100000005 bp) in 1.951 CPU sec, 1.960 real sec...
[M::mem_process_seqs] Processed 673472 reads in 180.666 CPU sec, 5.730 real sec
[M::mem_process_seqs] Processed 671056 reads in 111.057 CPU sec, 3.518 real sec
sorting block 97, #reads = 1065672 mem = 536879408 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278856, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 389, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (403.31, 173.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670800 sequences (100000003 bp) in 2.701 CPU sec, 2.814 real sec...
[M::process] read 670580 sequences (100000126 bp) in 1.590 CPU sec, 1.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 278674, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 390, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049)
[M::mem_pestat] mean and std.dev: (404.27, 174.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670924 reads in 171.451 CPU sec, 5.439 real sec
[M::mem_process_seqs] Processed 670888 reads in 111.650 CPU sec, 3.541 real sec
sorting block 98, #reads = 1072500 mem = 536877208 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277917, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 394, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1064)
[M::mem_pestat] mean and std.dev: (409.40, 177.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1329)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670538 sequences (100000098 bp) in 2.561 CPU sec, 2.710 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278822, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 401, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084)
[M::mem_pestat] mean and std.dev: (415.11, 180.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1355)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670666 sequences (100000168 bp) in 1.874 CPU sec, 1.884 real sec...
[M::mem_process_seqs] Processed 670800 reads in 171.740 CPU sec, 5.445 real sec
sorting block 99, #reads = 1070854 mem = 536878708 peak = 1102647296
[M::mem_process_seqs] Processed 670580 reads in 108.059 CPU sec, 3.408 real sec
sorting block 100, #reads = 1072091 mem = 536879184 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278786, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 401, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085)
[M::mem_pestat] mean and std.dev: (416.33, 181.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1356)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670582 sequences (100000300 bp) in 2.643 CPU sec, 2.786 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278836, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 400, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088)
[M::mem_pestat] mean and std.dev: (416.18, 182.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1360)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672038 sequences (100000241 bp) in 2.002 CPU sec, 2.004 real sec...
[M::mem_process_seqs] Processed 670538 reads in 168.368 CPU sec, 5.327 real sec
[M::mem_process_seqs] Processed 670666 reads in 109.206 CPU sec, 3.443 real sec
sorting block 101, #reads = 1071109 mem = 536879943 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278779, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 399, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090)
[M::mem_pestat] mean and std.dev: (417.53, 185.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1363)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672050 sequences (100000218 bp) in 2.598 CPU sec, 2.720 real sec...
[M::process] read 671484 sequences (100000084 bp) in 1.917 CPU sec, 1.926 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279183, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 379, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (398.15, 178.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1317)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670582 reads in 184.445 CPU sec, 5.857 real sec
[M::mem_process_seqs] Processed 672038 reads in 124.911 CPU sec, 3.956 real sec
sorting block 102, #reads = 1070542 mem = 536878900 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278066, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 384, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067)
[M::mem_pestat] mean and std.dev: (401.02, 179.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1337)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671394 sequences (100000061 bp) in 2.722 CPU sec, 2.832 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278657, 9, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 391, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068)
[M::mem_pestat] mean and std.dev: (407.58, 180.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1336)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671140 sequences (100000158 bp) in 1.960 CPU sec, 1.962 real sec...
[M::mem_process_seqs] Processed 672050 reads in 165.713 CPU sec, 5.237 real sec
[M::mem_process_seqs] Processed 671484 reads in 104.680 CPU sec, 3.299 real sec
sorting block 103, #reads = 1066582 mem = 536884578 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278490, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 392, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063)
[M::mem_pestat] mean and std.dev: (406.86, 179.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1329)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670544 sequences (100000226 bp) in 2.494 CPU sec, 2.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 278811, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (530, 530, 897)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1631)
[M::mem_pestat] mean and std.dev: (576.12, 121.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1998)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 391, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (405.68, 177.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1317)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 670552 sequences (100000020 bp) in 1.781 CPU sec, 1.789 real sec...
[M::mem_process_seqs] Processed 671394 reads in 161.202 CPU sec, 5.110 real sec
sorting block 104, #reads = 1073799 mem = 536877537 peak = 1102647296
[M::mem_process_seqs] Processed 671140 reads in 101.949 CPU sec, 3.212 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278051, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 397, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (410.37, 175.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 105, #reads = 1072870 mem = 536879788 peak = 1102647296
[M::process] read 670500 sequences (100000211 bp) in 2.759 CPU sec, 2.891 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278384, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 397, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050)
[M::mem_pestat] mean and std.dev: (411.69, 177.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670544 reads in 158.444 CPU sec, 5.014 real sec
[M::process] read 670376 sequences (100000253 bp) in 2.000 CPU sec, 2.007 real sec...
[M::mem_process_seqs] Processed 670552 reads in 103.018 CPU sec, 3.236 real sec
sorting block 106, #reads = 1072185 mem = 536879320 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278382, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 401, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (413.54, 177.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1322)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670950 sequences (100000014 bp) in 2.406 CPU sec, 2.530 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278437, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 404, 542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074)
[M::mem_pestat] mean and std.dev: (417.99, 181.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1340)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670500 reads in 152.769 CPU sec, 4.849 real sec
[M::process] read 672070 sequences (100000067 bp) in 1.975 CPU sec, 1.995 real sec...
[M::mem_process_seqs] Processed 670376 reads in 98.950 CPU sec, 3.106 real sec
sorting block 107, #reads = 1074754 mem = 536877548 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278716, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 395, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056)
[M::mem_pestat] mean and std.dev: (409.59, 178.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1318)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671370 sequences (100000279 bp) in 2.757 CPU sec, 2.887 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278997, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 378, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (395.42, 175.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670950 reads in 157.246 CPU sec, 4.988 real sec
[M::mem_process_seqs] Processed 672070 reads in 102.432 CPU sec, 3.218 real sec
[M::process] read 671112 sequences (100000094 bp) in 2.129 CPU sec, 2.151 real sec...
sorting block 108, #reads = 1073813 mem = 536882313 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278535, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 388, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (402.91, 176.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671096 sequences (100000023 bp) in 2.490 CPU sec, 2.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278576, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 393, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056)
[M::mem_pestat] mean and std.dev: (407.46, 177.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1319)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670794 sequences (100000006 bp) in 1.490 CPU sec, 1.487 real sec...
[M::mem_process_seqs] Processed 671370 reads in 160.491 CPU sec, 5.098 real sec
sorting block 109, #reads = 1074935 mem = 536877434 peak = 1102647296
[M::mem_process_seqs] Processed 671112 reads in 102.779 CPU sec, 3.247 real sec
sorting block 110, #reads = 1072920 mem = 536879584 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279377, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 392, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056)
[M::mem_pestat] mean and std.dev: (406.78, 177.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1319)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670548 sequences (100000027 bp) in 2.744 CPU sec, 2.874 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278866, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 398, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069)
[M::mem_pestat] mean and std.dev: (412.80, 180.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1335)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670420 sequences (100000271 bp) in 1.660 CPU sec, 1.661 real sec...
[M::mem_process_seqs] Processed 671096 reads in 162.610 CPU sec, 5.150 real sec
[M::mem_process_seqs] Processed 670794 reads in 104.819 CPU sec, 3.310 real sec
sorting block 111, #reads = 1072538 mem = 536880435 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 278035, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 399, 541)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079)
[M::mem_pestat] mean and std.dev: (415.44, 181.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1348)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670870 sequences (100000006 bp) in 2.395 CPU sec, 2.523 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278818, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 401, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083)
[M::mem_pestat] mean and std.dev: (417.07, 183.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1353)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671370 sequences (100000257 bp) in 1.880 CPU sec, 1.873 real sec...
[M::mem_process_seqs] Processed 670548 reads in 158.731 CPU sec, 5.029 real sec
[M::mem_process_seqs] Processed 670420 reads in 101.504 CPU sec, 3.196 real sec
sorting block 112, #reads = 1073483 mem = 536881431 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279284, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 393, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063)
[M::mem_pestat] mean and std.dev: (410.14, 180.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1328)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671010 sequences (100000207 bp) in 2.425 CPU sec, 2.519 real sec...
[M::process] read 670888 sequences (100000093 bp) in 1.700 CPU sec, 1.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279564, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 385, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (403.05, 178.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1314)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670870 reads in 169.076 CPU sec, 5.366 real sec
sorting block 113, #reads = 1073856 mem = 536878527 peak = 1102647296
[M::mem_process_seqs] Processed 671370 reads in 110.463 CPU sec, 3.506 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278714, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 392, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079)
[M::mem_pestat] mean and std.dev: (410.97, 183.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1350)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670700 sequences (100000244 bp) in 2.643 CPU sec, 2.783 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278403, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 396, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084)
[M::mem_pestat] mean and std.dev: (413.31, 183.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1356)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670932 sequences (100000290 bp) in 1.858 CPU sec, 1.855 real sec...
[M::mem_process_seqs] Processed 671010 reads in 165.767 CPU sec, 5.251 real sec
sorting block 114, #reads = 1070073 mem = 536885513 peak = 1102647296
[M::mem_process_seqs] Processed 670888 reads in 107.798 CPU sec, 3.393 real sec
sorting block 115, #reads = 1071074 mem = 536878810 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278650, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 395, 541)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087)
[M::mem_pestat] mean and std.dev: (413.30, 183.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1360)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670880 sequences (100000134 bp) in 2.374 CPU sec, 2.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278726, 8, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 670886 sequences (100000251 bp) in 1.840 CPU sec, 1.836 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (265, 392, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1087)
[M::mem_pestat] mean and std.dev: (411.27, 184.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1361)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670700 reads in 164.138 CPU sec, 5.204 real sec
[M::process] read 671362 sequences (100000240 bp) in 1.884 CPU sec, 1.897 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278603, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 397, 547)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107)
[M::mem_pestat] mean and std.dev: (417.02, 188.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1387)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670880 reads in 94.650 CPU sec, 3.243 real sec
sorting block 116, #reads = 1072036 mem = 536877021 peak = 1102647296
[M::mem_process_seqs] Processed 670932 reads in 224.012 CPU sec, 7.351 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 279028, 4, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (535, 2007, 5543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15559)
[M::mem_pestat] mean and std.dev: (2941.64, 2409.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 20567)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 392, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090)
[M::mem_pestat] mean and std.dev: (412.32, 185.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1365)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 117, #reads = 1070510 mem = 536877652 peak = 1102647296
[M::process] read 671714 sequences (100000261 bp) in 2.471 CPU sec, 2.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279364, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 385, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075)
[M::mem_pestat] mean and std.dev: (406.91, 183.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1346)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670886 reads in 181.533 CPU sec, 5.752 real sec
[M::mem_process_seqs] Processed 671362 reads in 108.195 CPU sec, 3.396 real sec
[M::process] read 673636 sequences (100000237 bp) in 2.218 CPU sec, 2.261 real sec...
sorting block 118, #reads = 1070287 mem = 536878470 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278769, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 380, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (401.41, 181.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1327)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672456 sequences (100000053 bp) in 2.774 CPU sec, 2.955 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279516, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 363, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (384.64, 176.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671828 sequences (100000114 bp) in 1.720 CPU sec, 1.719 real sec...
[M::mem_process_seqs] Processed 671714 reads in 175.486 CPU sec, 5.561 real sec
[M::mem_process_seqs] Processed 673636 reads in 108.198 CPU sec, 3.424 real sec
sorting block 119, #reads = 1064783 mem = 536879737 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279540, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 378, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (394.83, 175.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671336 sequences (100000071 bp) in 2.727 CPU sec, 2.865 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279575, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 387, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050)
[M::mem_pestat] mean and std.dev: (401.07, 176.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671350 sequences (100000248 bp) in 1.650 CPU sec, 1.647 real sec...
[M::mem_process_seqs] Processed 672456 reads in 155.322 CPU sec, 4.913 real sec
[M::mem_process_seqs] Processed 671828 reads in 98.270 CPU sec, 3.097 real sec
sorting block 120, #reads = 1067334 mem = 536884880 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279776, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 392, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (403.76, 174.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670752 sequences (100000097 bp) in 2.451 CPU sec, 2.581 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279297, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 389, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (403.53, 175.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670760 sequences (100000075 bp) in 1.860 CPU sec, 1.855 real sec...
[M::mem_process_seqs] Processed 671336 reads in 159.577 CPU sec, 5.054 real sec
sorting block 121, #reads = 1069185 mem = 536882444 peak = 1102647296
[M::mem_process_seqs] Processed 671350 reads in 103.292 CPU sec, 3.253 real sec
sorting block 122, #reads = 1070430 mem = 536879778 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278506, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 395, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (405.94, 171.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670556 sequences (100000246 bp) in 2.686 CPU sec, 2.807 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279244, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 400, 534)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056)
[M::mem_pestat] mean and std.dev: (410.34, 173.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1317)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (852, 852, 1078)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (400, 1530)
[M::mem_pestat] mean and std.dev: (919.80, 103.57)
[M::mem_pestat] low and high boundaries for proper pairs: (174, 1756)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 671478 sequences (100000084 bp) in 1.728 CPU sec, 1.724 real sec...
[M::mem_process_seqs] Processed 670752 reads in 162.301 CPU sec, 5.141 real sec
[M::mem_process_seqs] Processed 670760 reads in 103.784 CPU sec, 3.274 real sec
sorting block 123, #reads = 1068122 mem = 536879155 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278362, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 400, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059)
[M::mem_pestat] mean and std.dev: (411.40, 174.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671086 sequences (100000216 bp) in 2.445 CPU sec, 2.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279017, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 386, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (400.62, 173.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670922 sequences (100000031 bp) in 1.760 CPU sec, 1.756 real sec...
[M::mem_process_seqs] Processed 670556 reads in 156.429 CPU sec, 4.955 real sec
[M::mem_process_seqs] Processed 671478 reads in 98.721 CPU sec, 3.107 real sec
sorting block 124, #reads = 1070840 mem = 536878314 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278754, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 389, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (402.75, 171.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670484 sequences (100000224 bp) in 2.550 CPU sec, 2.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279043, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 390, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (403.29, 171.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671086 reads in 157.280 CPU sec, 4.983 real sec
[M::process] read 670822 sequences (100000207 bp) in 2.042 CPU sec, 2.055 real sec...
[M::mem_process_seqs] Processed 670922 reads in 100.690 CPU sec, 3.159 real sec
sorting block 125, #reads = 1072578 mem = 536880143 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278550, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 391, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (404.30, 170.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670978 sequences (100000299 bp) in 2.474 CPU sec, 2.602 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278655, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 384, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (397.54, 167.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670458 sequences (100000205 bp) in 1.640 CPU sec, 1.649 real sec...
[M::mem_process_seqs] Processed 670484 reads in 158.284 CPU sec, 5.022 real sec
sorting block 126, #reads = 1071365 mem = 536880203 peak = 1102647296
[M::mem_process_seqs] Processed 670822 reads in 102.190 CPU sec, 3.220 real sec
sorting block 127, #reads = 1070872 mem = 536877882 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278454, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (394.34, 166.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670828 sequences (100000158 bp) in 2.583 CPU sec, 2.750 real sec...
[M::process] read 670594 sequences (100000172 bp) in 1.510 CPU sec, 1.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278465, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 382, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (398.59, 169.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670978 reads in 168.192 CPU sec, 5.336 real sec
[M::mem_process_seqs] Processed 670458 reads in 107.099 CPU sec, 3.391 real sec
sorting block 128, #reads = 1069109 mem = 536880244 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277951, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 381, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (397.65, 169.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670634 sequences (100000207 bp) in 2.049 CPU sec, 2.171 real sec...
[M::process] read 670756 sequences (100000021 bp) in 1.400 CPU sec, 1.398 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278348, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 381, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (397.55, 169.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670828 reads in 156.291 CPU sec, 4.972 real sec
[M::mem_process_seqs] Processed 670594 reads in 97.951 CPU sec, 3.107 real sec
sorting block 129, #reads = 1071247 mem = 536882631 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277151, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 379, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (395.89, 168.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670650 sequences (100000260 bp) in 2.352 CPU sec, 2.467 real sec...
[M::process] read 670580 sequences (100000269 bp) in 1.410 CPU sec, 1.410 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278001, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 379, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (397.19, 170.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670634 reads in 154.322 CPU sec, 4.904 real sec
sorting block 130, #reads = 1072767 mem = 536886873 peak = 1102647296
[M::mem_process_seqs] Processed 670756 reads in 98.744 CPU sec, 3.128 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278013, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 378, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (396.70, 171.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670598 sequences (100000196 bp) in 2.482 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277823, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 381, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (399.67, 172.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670650 reads in 160.405 CPU sec, 5.083 real sec
[M::process] read 670352 sequences (100000012 bp) in 1.889 CPU sec, 1.886 real sec...
sorting block 131, #reads = 1072893 mem = 536879001 peak = 1102647296
[M::mem_process_seqs] Processed 670580 reads in 101.310 CPU sec, 3.187 real sec
sorting block 132, #reads = 1073212 mem = 536878858 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277517, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 380, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (398.16, 170.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670538 sequences (100000283 bp) in 2.506 CPU sec, 2.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277435, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 381, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (399.50, 171.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670598 reads in 156.489 CPU sec, 4.959 real sec
[M::process] read 670370 sequences (100000038 bp) in 1.789 CPU sec, 1.792 real sec...
[M::mem_process_seqs] Processed 670352 reads in 98.094 CPU sec, 3.087 real sec
sorting block 133, #reads = 1074152 mem = 536881046 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 277199, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 384, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (402.32, 174.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670544 sequences (100000278 bp) in 2.437 CPU sec, 2.553 real sec...
[M::process] read 69182 sequences (10298335 bp) in 0.440 CPU sec, 0.441 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278390, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 388, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (406.53, 176.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670538 reads in 149.334 CPU sec, 4.768 real sec
[M::mem_process_seqs] Processed 670370 reads in 94.359 CPU sec, 3.008 real sec
sorting block 134, #reads = 1074013 mem = 536876884 peak = 1102647296
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278212, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 389, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (406.67, 176.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 28677, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 379, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (395.72, 173.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670544 reads in 91.943 CPU sec, 2.984 real sec
[M::mem_process_seqs] Processed 69182 reads in 23.415 CPU sec, 0.756 real sec
sorting block 135, #reads = 1074282 mem = 536881986 peak = 1102647296
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 145640254
overall: 69827649536 mem 20529.692 user 420.987 sys 680.502 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_Kh-T1-TDNA-1_A23MHWWLT4_1.L001\tPU:L001\tSM:659_Kh-T1-TDNA-1_A23MHWWLT4_1\tLB:659_Kh-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_Kh-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_Kh-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 680.544 sec; CPU: 20950.682 sec
sorting block 136, #reads = 404566 mem = 202955789 peak = 1102647296
uniq header count 1
merging 137 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8170375523
output reads: 146350164
bam_mem_sort: 137 calls 78.390 user 1.946 sys 80.696 real
bam_write: 137 calls 35.828 user 2.396 sys 39.105 real
execute: 1 calls 39.314 user 1.277 sys 734.264 real
merge_files: 1 calls 39.297 user 1.251 sys 53.221 real
parse_chunk: 2183 calls 88.370 user 21.564 sys 111.490 real
read_chunk: 5878 calls 47.316 user 45.540 sys 634.853 real
sort_block: 1 calls 31.985 user 45.156 sys 611.465 real
write_chunk: 3693 calls 38.922 user 0.888 sys 52.201 real
overall: 3176697856 mem 2063.978 user 89.848 sys 734.286 real
ls: cannot access '*.cram': No such file or directory