This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_3U6-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2096.040 CPU sec, 92.158 real sec
[M::process] read 686790 sequences (100000155 bp) in 1.232 CPU sec, 1.242 real sec...
[M::process] read 685408 sequences (100000039 bp) in 1.508 CPU sec, 1.501 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283665, 5, 25)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 302, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (320.67, 159.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 297, 840)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1984)
[M::mem_pestat] mean and std.dev: (525.17, 559.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2765)
[M::mem_pestat] skip orientation RR
[M::process] read 684044 sequences (100000243 bp) in 1.536 CPU sec, 1.541 real sec...
[M::process] read 683120 sequences (100000081 bp) in 1.411 CPU sec, 1.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283313, 6, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 338, 604)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1454)
[M::mem_pestat] mean and std.dev: (386.10, 223.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1879)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 310, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (327.22, 160.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 295, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555)
[M::mem_pestat] mean and std.dev: (287.75, 83.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 671)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686790 reads in 186.129 CPU sec, 5.984 real sec
[M::mem_process_seqs] Processed 685408 reads in 115.816 CPU sec, 3.822 real sec
sorting block 0, #reads = 1082237 mem = 536878870 peak = 570990592
[M::process] read 682584 sequences (100000248 bp) in 2.049 CPU sec, 2.194 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282880, 2, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 204, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1284)
[M::mem_pestat] mean and std.dev: (250.38, 172.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1675)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 317, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (332.33, 160.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 252, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520)
[M::mem_pestat] mean and std.dev: (234.71, 75.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 636)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682796 sequences (100000073 bp) in 1.684 CPU sec, 1.697 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282637, 3, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 298, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837)
[M::mem_pestat] mean and std.dev: (277.19, 87.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1051)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 323, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (338.50, 163.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (380, 478, 556)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (28, 908)
[M::mem_pestat] mean and std.dev: (426.50, 148.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684044 reads in 200.340 CPU sec, 6.615 real sec
[M::mem_process_seqs] Processed 683120 reads in 128.938 CPU sec, 4.259 real sec
sorting block 1, #reads = 1080194 mem = 536879347 peak = 699965440
[M::process] read 682272 sequences (100000125 bp) in 1.984 CPU sec, 2.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 282714, 1, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 305, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1198)
[M::mem_pestat] mean and std.dev: (345.79, 155.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1534)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 324, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (338.32, 162.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (402, 438, 670)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206)
[M::mem_pestat] mean and std.dev: (499.39, 267.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1569)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682250 sequences (100000045 bp) in 1.776 CPU sec, 1.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282533, 6, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 268, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1143)
[M::mem_pestat] mean and std.dev: (292.60, 154.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1463)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 322, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (338.42, 163.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (114, 197, 367)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (213.96, 124.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282694, 7, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 276, 928)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2506)
[M::mem_pestat] mean and std.dev: (409.71, 340.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3295)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 326, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (340.58, 162.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 273, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (332.36, 167.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682584 reads in 314.961 CPU sec, 10.687 real sec
[M::mem_process_seqs] Processed 682796 reads in 234.808 CPU sec, 7.979 real sec
sorting block 2, #reads = 1079819 mem = 536879668 peak = 804601856
[M::mem_process_seqs] Processed 682272 reads in 252.973 CPU sec, 8.605 real sec
[M::process] read 682314 sequences (100000046 bp) in 2.460 CPU sec, 2.658 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 281761, 10, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 179, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (279.86, 204.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 321, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (335.86, 159.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (288, 1072, 1280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3264)
[M::mem_pestat] mean and std.dev: (824.70, 460.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4256)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (244.00, 79.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 3, #reads = 1078848 mem = 536879689 peak = 804601856
[M::process] read 682254 sequences (100000078 bp) in 2.293 CPU sec, 2.468 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 282091, 3, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 379, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (362.36, 215.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 322, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (336.20, 160.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (291, 421, 1159)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2895)
[M::mem_pestat] mean and std.dev: (587.58, 435.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3763)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681986 sequences (100000036 bp) in 1.744 CPU sec, 1.749 real sec...
[M::mem_process_seqs] Processed 682250 reads in 227.910 CPU sec, 7.478 real sec
[M::mem_process_seqs] Processed 682314 reads in 140.536 CPU sec, 4.547 real sec
sorting block 4, #reads = 1079033 mem = 536877414 peak = 804601856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 281794, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (223, 268, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721)
[M::mem_pestat] mean and std.dev: (272.52, 100.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 322, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (336.80, 160.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 367, 689)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1627)
[M::mem_pestat] mean and std.dev: (481.79, 293.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2096)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684224 sequences (100000153 bp) in 2.545 CPU sec, 2.728 real sec...
[M::process] read 685686 sequences (100000104 bp) in 1.827 CPU sec, 1.843 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 282262, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 316, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (277.69, 93.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1083)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (341.68, 164.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 428, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159)
[M::mem_pestat] mean and std.dev: (328.25, 144.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1476)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682254 reads in 199.680 CPU sec, 6.399 real sec
[M::mem_process_seqs] Processed 681986 reads in 126.581 CPU sec, 4.043 real sec
sorting block 5, #reads = 1077821 mem = 536879170 peak = 804601856
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283205, 7, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (287, 373, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (397.00, 147.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 316, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (333.21, 163.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 218, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (229.47, 122.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684444 sequences (100000084 bp) in 2.187 CPU sec, 2.378 real sec...
sorting block 6, #reads = 1076944 mem = 536878121 peak = 804601856
[M::process] read 683524 sequences (100000169 bp) in 1.631 CPU sec, 1.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283658, 0, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 290, 606)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1502)
[M::mem_pestat] mean and std.dev: (434.95, 341.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1950)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 308, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (324.88, 159.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 262, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (277.33, 97.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684224 reads in 187.471 CPU sec, 6.012 real sec
[M::process] read 683046 sequences (100000088 bp) in 1.647 CPU sec, 1.655 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 282897, 8, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (275, 312, 801)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1853)
[M::mem_pestat] mean and std.dev: (539.76, 475.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2443)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 314, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (328.88, 158.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 235, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (275.19, 139.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684444 reads in 105.187 CPU sec, 3.556 real sec
sorting block 7, #reads = 1081314 mem = 536885482 peak = 804601856
[M::mem_process_seqs] Processed 685686 reads in 255.375 CPU sec, 8.375 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283041, 3, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (266, 294, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (78, 548)
[M::mem_pestat] mean and std.dev: (281.94, 80.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 642)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 318, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (333.31, 160.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 251, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (258.79, 77.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682616 sequences (100000044 bp) in 2.205 CPU sec, 2.350 real sec...
[M::process] read 682272 sequences (100000079 bp) in 1.453 CPU sec, 1.456 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 282691, 4, 22)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 254, 592)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1416)
[M::mem_pestat] mean and std.dev: (250.88, 136.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1828)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 324, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (337.51, 161.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 377, 2391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6797)
[M::mem_pestat] mean and std.dev: (1335.86, 1626.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9000)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683524 reads in 200.361 CPU sec, 6.432 real sec
sorting block 8, #reads = 1082657 mem = 536879166 peak = 804601856
[M::mem_process_seqs] Processed 683046 reads in 117.216 CPU sec, 3.767 real sec
sorting block 9, #reads = 1080387 mem = 536880176 peak = 848302080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282126, 5, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 230, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584)
[M::mem_pestat] mean and std.dev: (237.92, 70.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 724)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 325, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (339.15, 162.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 232, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (23, 488)
[M::mem_pestat] mean and std.dev: (234.21, 59.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 581)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682046 sequences (100000255 bp) in 2.189 CPU sec, 2.336 real sec...
[M::process] read 682602 sequences (100000195 bp) in 1.769 CPU sec, 1.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282311, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 212, 216)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (96, 296)
[M::mem_pestat] mean and std.dev: (181.33, 31.50)
[M::mem_pestat] low and high boundaries for proper pairs: (55, 336)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 324, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (337.72, 161.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 304, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (338.46, 134.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682616 reads in 185.185 CPU sec, 5.924 real sec
[M::mem_process_seqs] Processed 682272 reads in 118.290 CPU sec, 3.776 real sec
sorting block 10, #reads = 1079196 mem = 536881375 peak = 848302080
[M::process] read 682346 sequences (100000055 bp) in 2.154 CPU sec, 2.303 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282158, 6, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 253, 282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512)
[M::mem_pestat] mean and std.dev: (224.93, 63.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 627)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (340.11, 161.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 241, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (312.72, 191.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1324)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682198 sequences (100000029 bp) in 2.006 CPU sec, 2.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 282176, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (264, 400, 681)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1515)
[M::mem_pestat] mean and std.dev: (408.35, 182.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1932)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 323, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (338.48, 162.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 324, 772)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1882)
[M::mem_pestat] mean and std.dev: (447.37, 271.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2437)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682046 reads in 182.260 CPU sec, 5.802 real sec
[M::mem_process_seqs] Processed 682602 reads in 112.957 CPU sec, 3.593 real sec
sorting block 11, #reads = 1079028 mem = 536877749 peak = 848302080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282212, 4, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (101, 204, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1373)
[M::mem_pestat] mean and std.dev: (226.75, 161.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1797)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 321, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (336.82, 161.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 342, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (305.15, 127.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682096 sequences (100000226 bp) in 2.376 CPU sec, 2.508 real sec...
[M::process] read 685410 sequences (100000161 bp) in 1.860 CPU sec, 1.854 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282430, 1, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1143)
[M::mem_pestat] mean and std.dev: (290.75, 147.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1463)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 323, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (337.80, 162.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 272, 657)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1473)
[M::mem_pestat] mean and std.dev: (401.72, 302.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1881)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682346 reads in 176.470 CPU sec, 5.615 real sec
sorting block 12, #reads = 1077953 mem = 536880832 peak = 848302080
[M::mem_process_seqs] Processed 682198 reads in 113.245 CPU sec, 3.594 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 282309, 3, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 240, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (277.00, 116.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (344.19, 166.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 363, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203)
[M::mem_pestat] mean and std.dev: (338.65, 253.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1537)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684766 sequences (100000152 bp) in 2.430 CPU sec, 2.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 283828, 4, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 230, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723)
[M::mem_pestat] mean and std.dev: (255.40, 135.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 915)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 308, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (326.14, 160.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 345, 1158)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3132)
[M::mem_pestat] mean and std.dev: (817.12, 1017.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4886)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684066 sequences (100000194 bp) in 1.810 CPU sec, 1.810 real sec...
[M::mem_process_seqs] Processed 682096 reads in 169.686 CPU sec, 5.388 real sec
sorting block 13, #reads = 1077011 mem = 536878223 peak = 848302080
[M::mem_process_seqs] Processed 685410 reads in 105.366 CPU sec, 3.336 real sec
sorting block 14, #reads = 1078929 mem = 536876321 peak = 935104512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283285, 8, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 338, 853)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2183)
[M::mem_pestat] mean and std.dev: (566.41, 519.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2848)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 310, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (326.76, 159.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 263, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (44, 534)
[M::mem_pestat] mean and std.dev: (262.73, 76.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 632)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683484 sequences (100000216 bp) in 2.482 CPU sec, 2.622 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283234, 6, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 266, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (282.62, 123.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 317, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (332.00, 160.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 316, 568)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1338)
[M::mem_pestat] mean and std.dev: (323.57, 160.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1723)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682544 sequences (100000263 bp) in 1.720 CPU sec, 1.724 real sec...
[M::mem_process_seqs] Processed 684766 reads in 168.526 CPU sec, 5.356 real sec
[M::process] read 682100 sequences (100000166 bp) in 2.010 CPU sec, 2.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282976, 4, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 352, 3119)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8903)
[M::mem_pestat] mean and std.dev: (1235.67, 1268.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11795)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 323, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (337.29, 163.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 222, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (21, 511)
[M::mem_pestat] mean and std.dev: (230.62, 49.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 609)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683484 reads in 97.119 CPU sec, 3.327 real sec
sorting block 15, #reads = 1082344 mem = 536877319 peak = 935104512
[M::mem_process_seqs] Processed 684066 reads in 241.362 CPU sec, 7.910 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282469, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 328, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (313.48, 151.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (341.57, 164.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 402, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (343.67, 171.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1342)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 16, #reads = 1081002 mem = 536878900 peak = 935104512
[M::process] read 682008 sequences (100000195 bp) in 2.622 CPU sec, 2.794 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282109, 5, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (254, 275, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (309.52, 110.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (342.59, 164.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (279, 309, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (338.47, 154.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682544 reads in 185.556 CPU sec, 5.904 real sec
[M::process] read 682136 sequences (100000301 bp) in 1.518 CPU sec, 1.538 real sec...
[M::mem_process_seqs] Processed 682100 reads in 106.794 CPU sec, 3.371 real sec
sorting block 17, #reads = 1079269 mem = 536881289 peak = 935104512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 282407, 7, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (180, 368, 627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1521)
[M::mem_pestat] mean and std.dev: (395.12, 325.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1968)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 326, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (341.05, 163.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 314, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1141)
[M::mem_pestat] mean and std.dev: (323.44, 151.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1455)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682676 sequences (100000050 bp) in 2.360 CPU sec, 2.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 281891, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (276, 294, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (164, 444)
[M::mem_pestat] mean and std.dev: (285.24, 53.15)
[M::mem_pestat] low and high boundaries for proper pairs: (73, 500)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 323, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (338.90, 162.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 243, 1314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3632)
[M::mem_pestat] mean and std.dev: (658.00, 811.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4791)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682350 sequences (100000161 bp) in 1.790 CPU sec, 1.793 real sec...
[M::mem_process_seqs] Processed 682008 reads in 165.388 CPU sec, 5.260 real sec
[M::mem_process_seqs] Processed 682136 reads in 106.434 CPU sec, 3.370 real sec
sorting block 18, #reads = 1079266 mem = 536879159 peak = 935104512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 282780, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 488, 706)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1668)
[M::mem_pestat] mean and std.dev: (428.17, 239.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2149)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 319, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (335.73, 161.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 682022 sequences (100000111 bp) in 2.422 CPU sec, 2.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 282479, 4, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 308, 550)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1218)
[M::mem_pestat] mean and std.dev: (337.35, 176.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1552)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 319, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (335.79, 161.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 233, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (266.96, 109.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681666 sequences (100000173 bp) in 1.850 CPU sec, 1.854 real sec...
[M::mem_process_seqs] Processed 682676 reads in 165.733 CPU sec, 5.260 real sec
[M::mem_process_seqs] Processed 682350 reads in 103.886 CPU sec, 3.283 real sec
sorting block 19, #reads = 1078940 mem = 536877948 peak = 935104512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283004, 8, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 229, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1170)
[M::mem_pestat] mean and std.dev: (331.87, 229.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1495)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 324, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (340.93, 165.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 253, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (259.00, 111.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685774 sequences (100000205 bp) in 2.379 CPU sec, 2.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282639, 8, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (228, 493, 1056)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2712)
[M::mem_pestat] mean and std.dev: (525.65, 327.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3540)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (348.00, 169.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 231, 794)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2096)
[M::mem_pestat] mean and std.dev: (461.20, 437.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2747)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684176 sequences (100000259 bp) in 1.780 CPU sec, 1.786 real sec...
[M::mem_process_seqs] Processed 682022 reads in 160.524 CPU sec, 5.100 real sec
sorting block 20, #reads = 1078731 mem = 536877507 peak = 935104512
[M::mem_process_seqs] Processed 681666 reads in 100.793 CPU sec, 3.177 real sec
sorting block 21, #reads = 1078548 mem = 536880490 peak = 935104512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 283715, 3, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 218, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (266.95, 191.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 306, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (325.08, 160.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 313, 853)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2177)
[M::mem_pestat] mean and std.dev: (395.94, 281.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2839)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683358 sequences (100000055 bp) in 2.504 CPU sec, 2.662 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283315, 8, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 227, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538)
[M::mem_pestat] mean and std.dev: (220.56, 60.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 653)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 317, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (333.81, 163.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 301, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079)
[M::mem_pestat] mean and std.dev: (294.54, 152.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1387)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685774 reads in 160.219 CPU sec, 5.092 real sec
[M::process] read 682872 sequences (100000019 bp) in 1.926 CPU sec, 1.939 real sec...
[M::mem_process_seqs] Processed 684176 reads in 100.056 CPU sec, 3.149 real sec
sorting block 22, #reads = 1080759 mem = 536880946 peak = 935104512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282863, 2, 28)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 321, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (337.16, 163.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (338, 480, 764)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1616)
[M::mem_pestat] mean and std.dev: (450.79, 236.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2042)
[M::mem_pestat] skip orientation RR
[M::process] read 682360 sequences (100000152 bp) in 2.322 CPU sec, 2.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282048, 17, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 162, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (201.19, 93.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (342.73, 166.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (246, 1276, 1502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4014)
[M::mem_pestat] mean and std.dev: (899.73, 549.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5270)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 274, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827)
[M::mem_pestat] mean and std.dev: (266.71, 99.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682442 sequences (100000155 bp) in 1.650 CPU sec, 1.650 real sec...
[M::mem_process_seqs] Processed 683358 reads in 163.703 CPU sec, 5.211 real sec
[M::mem_process_seqs] Processed 682872 reads in 104.704 CPU sec, 3.311 real sec
sorting block 23, #reads = 1082388 mem = 536880218 peak = 935104512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 281968, 6, 26)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 193, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (314.15, 208.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (344.75, 166.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 383, 622)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1476)
[M::mem_pestat] mean and std.dev: (311.05, 170.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1903)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681996 sequences (100000034 bp) in 2.469 CPU sec, 2.610 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 281848, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 364, 706)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1676)
[M::mem_pestat] mean and std.dev: (489.12, 335.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2161)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (342.47, 164.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 682930 sequences (100000076 bp) in 1.790 CPU sec, 1.797 real sec...
[M::mem_process_seqs] Processed 682360 reads in 166.023 CPU sec, 5.273 real sec
sorting block 24, #reads = 1081245 mem = 536878534 peak = 935104512
[M::mem_process_seqs] Processed 682442 reads in 105.152 CPU sec, 3.320 real sec
sorting block 25, #reads = 1079095 mem = 536878603 peak = 935104512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 282290, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 269, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730)
[M::mem_pestat] mean and std.dev: (250.74, 126.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 927)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (341.56, 163.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 511, 835)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2161)
[M::mem_pestat] mean and std.dev: (456.92, 294.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2824)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682770 sequences (100000089 bp) in 2.027 CPU sec, 2.144 real sec...
[M::process] read 682450 sequences (100000227 bp) in 1.420 CPU sec, 1.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282279, 8, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 321, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (305.62, 152.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1327)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 317, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (333.32, 160.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 565, 669)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1569)
[M::mem_pestat] mean and std.dev: (433.31, 214.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2019)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681996 reads in 163.346 CPU sec, 5.213 real sec
[M::mem_process_seqs] Processed 682930 reads in 101.884 CPU sec, 3.236 real sec
sorting block 26, #reads = 1079054 mem = 536878418 peak = 935104512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282743, 2, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 666, 1364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3744)
[M::mem_pestat] mean and std.dev: (660.69, 460.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4934)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 318, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (333.76, 160.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 327, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617)
[M::mem_pestat] mean and std.dev: (291.21, 93.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681876 sequences (100000242 bp) in 2.552 CPU sec, 2.687 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 282475, 0, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (232, 416, 930)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2326)
[M::mem_pestat] mean and std.dev: (379.18, 238.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3024)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 320, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (336.08, 161.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 352, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738)
[M::mem_pestat] mean and std.dev: (265.86, 110.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682770 reads in 161.481 CPU sec, 5.130 real sec
[M::process] read 682280 sequences (100000179 bp) in 1.894 CPU sec, 1.895 real sec...
[M::mem_process_seqs] Processed 682450 reads in 102.237 CPU sec, 3.217 real sec
sorting block 27, #reads = 1079491 mem = 536879775 peak = 935104512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 282842, 2, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (144, 246, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (343.87, 225.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 325, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (341.36, 164.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 296, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (289.48, 93.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1037)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 690284 sequences (100000276 bp) in 2.485 CPU sec, 2.591 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 282633, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 215, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 619)
[M::mem_pestat] mean and std.dev: (206.25, 92.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 325, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (342.31, 167.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 306, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (281.78, 69.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681876 reads in 158.045 CPU sec, 5.025 real sec
[M::process] read 687496 sequences (100000190 bp) in 1.930 CPU sec, 1.946 real sec...
[M::mem_process_seqs] Processed 682280 reads in 100.717 CPU sec, 3.174 real sec
sorting block 28, #reads = 1078815 mem = 536878402 peak = 935104512
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 285485, 3, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (241, 295, 625)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1393)
[M::mem_pestat] mean and std.dev: (437.35, 244.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1777)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 283, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (308.42, 157.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 203, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1176)
[M::mem_pestat] mean and std.dev: (284.43, 197.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1514)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685154 sequences (100000247 bp) in 2.659 CPU sec, 2.842 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 285058, 0, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 562, 1158)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3036)
[M::mem_pestat] mean and std.dev: (707.57, 573.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3975)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 299, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (320.00, 160.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 333, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (299.41, 89.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 690284 reads in 169.103 CPU sec, 5.374 real sec
[M::process] read 684548 sequences (100000130 bp) in 1.886 CPU sec, 1.890 real sec...
sorting block 29, #reads = 1078633 mem = 536877271 peak = 935104512
[M::mem_process_seqs] Processed 687496 reads in 106.328 CPU sec, 3.355 real sec
sorting block 30, #reads = 1080735 mem = 536881190 peak = 966217728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283667, 4, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 297, 424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (273.92, 135.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 315, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (332.56, 164.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 363, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 605)
[M::mem_pestat] mean and std.dev: (312.38, 109.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684328 sequences (100000065 bp) in 2.304 CPU sec, 2.413 real sec...
[M::process] read 684242 sequences (100000110 bp) in 1.790 CPU sec, 1.786 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 283403, 6, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 326, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148)
[M::mem_pestat] mean and std.dev: (331.88, 151.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1458)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 320, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (336.19, 165.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (285, 384, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (73, 603)
[M::mem_pestat] mean and std.dev: (341.27, 96.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685154 reads in 166.124 CPU sec, 5.279 real sec
[M::mem_process_seqs] Processed 684548 reads in 105.685 CPU sec, 3.342 real sec
sorting block 31, #reads = 1078812 mem = 536880164 peak = 966217728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 284018, 9, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 288, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1198)
[M::mem_pestat] mean and std.dev: (344.48, 207.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1537)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 318, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (333.92, 162.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 367, 734)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1886)
[M::mem_pestat] mean and std.dev: (424.27, 323.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2462)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683686 sequences (100000168 bp) in 2.529 CPU sec, 2.677 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283260, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 273, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (220.31, 72.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 664)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 318, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (333.39, 162.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 321, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (352.46, 209.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683026 sequences (100000237 bp) in 1.760 CPU sec, 1.759 real sec...
[M::mem_process_seqs] Processed 684328 reads in 167.998 CPU sec, 5.340 real sec
[M::mem_process_seqs] Processed 684242 reads in 105.662 CPU sec, 3.338 real sec
sorting block 32, #reads = 1076250 mem = 536880497 peak = 966217728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 283082, 4, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 253, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (272.89, 114.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (336.53, 162.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 275, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (306.55, 178.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682580 sequences (100000180 bp) in 2.193 CPU sec, 2.302 real sec...
[M::process] read 682274 sequences (100000199 bp) in 1.420 CPU sec, 1.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 282895, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 316, 561)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1313)
[M::mem_pestat] mean and std.dev: (342.93, 274.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1689)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (339.39, 163.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 302, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (299.41, 103.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683686 reads in 170.075 CPU sec, 5.427 real sec
sorting block 33, #reads = 1076227 mem = 536880220 peak = 966217728
[M::process] read 689330 sequences (100000160 bp) in 1.878 CPU sec, 1.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 282790, 5, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 293, 563)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1339)
[M::mem_pestat] mean and std.dev: (284.40, 164.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1727)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 326, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (341.47, 164.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 235, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843)
[M::mem_pestat] mean and std.dev: (278.67, 127.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1049)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682580 reads in 98.837 CPU sec, 3.180 real sec
sorting block 34, #reads = 1076164 mem = 536878434 peak = 966217728
[M::mem_process_seqs] Processed 683026 reads in 231.905 CPU sec, 7.416 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282330, 6, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (289, 559, 1266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3220)
[M::mem_pestat] mean and std.dev: (743.31, 564.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4197)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 330, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (345.20, 167.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 391, 796)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2024)
[M::mem_pestat] mean and std.dev: (438.62, 380.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2638)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 690072 sequences (100000137 bp) in 2.677 CPU sec, 2.821 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 285521, 5, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 224, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541)
[M::mem_pestat] mean and std.dev: (235.47, 93.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 662)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 288, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (312.45, 158.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (331, 418, 1286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3196)
[M::mem_pestat] mean and std.dev: (548.67, 461.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4151)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682274 reads in 192.711 CPU sec, 6.105 real sec
[M::process] read 689010 sequences (100000238 bp) in 1.922 CPU sec, 1.930 real sec...
[M::mem_process_seqs] Processed 689330 reads in 115.899 CPU sec, 3.655 real sec
sorting block 35, #reads = 1075969 mem = 536879193 peak = 966217728
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 286067, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 308, 675)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1595)
[M::mem_pestat] mean and std.dev: (446.64, 289.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2055)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 282, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861)
[M::mem_pestat] mean and std.dev: (304.64, 151.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 687076 sequences (100000279 bp) in 2.704 CPU sec, 2.869 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 285720, 1, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 259, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (263.88, 135.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 867)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 289, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (308.36, 151.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 149, 938)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2562)
[M::mem_pestat] mean and std.dev: (409.14, 456.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3374)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686292 sequences (100000177 bp) in 1.880 CPU sec, 1.878 real sec...
[M::mem_process_seqs] Processed 690072 reads in 179.056 CPU sec, 5.681 real sec
sorting block 36, #reads = 1075874 mem = 536881680 peak = 966217728
[M::mem_process_seqs] Processed 689010 reads in 114.069 CPU sec, 3.614 real sec
sorting block 37, #reads = 1075648 mem = 536880728 peak = 980860928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 284648, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 233, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (239.39, 122.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 300, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (317.45, 155.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (328, 394, 568)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (393.41, 183.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685934 sequences (100000016 bp) in 2.552 CPU sec, 2.676 real sec...
[M::process] read 685062 sequences (100000128 bp) in 1.860 CPU sec, 1.864 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 284276, 4, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 321, 765)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1925)
[M::mem_pestat] mean and std.dev: (458.24, 419.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2505)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 305, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (322.31, 157.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 261, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (261.31, 158.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1089)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687076 reads in 182.796 CPU sec, 5.805 real sec
[M::mem_process_seqs] Processed 686292 reads in 116.720 CPU sec, 3.701 real sec
sorting block 38, #reads = 1075225 mem = 536879029 peak = 980860928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284714, 4, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (119, 196, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280)
[M::mem_pestat] mean and std.dev: (245.87, 153.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1667)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 307, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (323.85, 157.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (336, 390, 625)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203)
[M::mem_pestat] mean and std.dev: (423.67, 202.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1492)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683472 sequences (100000141 bp) in 2.433 CPU sec, 2.536 real sec...
[M::process] read 683002 sequences (100000053 bp) in 1.840 CPU sec, 1.831 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 283652, 0, 21)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (432, 642, 728)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320)
[M::mem_pestat] mean and std.dev: (562.15, 213.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1616)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 313, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (330.84, 162.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 480, 1018)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2674)
[M::mem_pestat] mean and std.dev: (574.76, 421.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3502)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685934 reads in 175.141 CPU sec, 5.560 real sec
[M::mem_process_seqs] Processed 685062 reads in 110.779 CPU sec, 3.510 real sec
sorting block 39, #reads = 1074739 mem = 536878379 peak = 980860928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 282716, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 331, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (354.93, 191.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 325, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (342.50, 168.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 274, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096)
[M::mem_pestat] mean and std.dev: (358.88, 209.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1395)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682250 sequences (100000155 bp) in 2.559 CPU sec, 2.682 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283459, 1, 23)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 279, 914)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2340)
[M::mem_pestat] mean and std.dev: (448.62, 423.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3053)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 328, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (345.53, 170.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (336, 440, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (395.58, 163.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688606 sequences (100000054 bp) in 1.930 CPU sec, 1.933 real sec...
[M::mem_process_seqs] Processed 683472 reads in 174.547 CPU sec, 5.540 real sec
sorting block 40, #reads = 1076190 mem = 536879128 peak = 980860928
[M::mem_process_seqs] Processed 683002 reads in 109.992 CPU sec, 3.473 real sec
sorting block 41, #reads = 1075602 mem = 536877612 peak = 980860928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 283369, 8, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 297, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200)
[M::mem_pestat] mean and std.dev: (347.92, 237.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1544)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 333, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (349.66, 171.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 313, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (103, 453)
[M::mem_pestat] mean and std.dev: (283.27, 69.55)
[M::mem_pestat] low and high boundaries for proper pairs: (5, 561)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687146 sequences (100000236 bp) in 2.452 CPU sec, 2.559 real sec...
[M::process] read 685552 sequences (100000249 bp) in 1.580 CPU sec, 1.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 285602, 8, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 288, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (278.06, 125.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 292, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (315.83, 160.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 416, 881)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2251)
[M::mem_pestat] mean and std.dev: (546.17, 428.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2936)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682250 reads in 180.777 CPU sec, 5.752 real sec
[M::mem_process_seqs] Processed 688606 reads in 116.762 CPU sec, 3.703 real sec
sorting block 42, #reads = 1071542 mem = 536880047 peak = 980860928
[M::process] read 685078 sequences (100000046 bp) in 2.482 CPU sec, 2.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 284331, 10, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 270, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (301.67, 177.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 310, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (328.39, 162.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (805, 824, 824)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (767, 862)
[M::mem_pestat] mean and std.dev: (821.29, 6.65)
[M::mem_pestat] low and high boundaries for proper pairs: (748, 881)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 279, 637)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1525)
[M::mem_pestat] mean and std.dev: (364.28, 280.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1969)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 684836 sequences (100000282 bp) in 1.860 CPU sec, 1.860 real sec...
[M::mem_process_seqs] Processed 685552 reads in 91.064 CPU sec, 2.862 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 284259, 2, 27)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 245, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (274.79, 162.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 300, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (320.49, 159.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 392, 779)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1859)
[M::mem_pestat] mean and std.dev: (489.73, 327.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2399)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283810, 6, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 266, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (276.33, 112.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 315, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (332.95, 164.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (315, 403, 771)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1683)
[M::mem_pestat] mean and std.dev: (517.07, 305.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2139)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683966 sequences (100000236 bp) in 1.743 CPU sec, 1.749 real sec...
[M::mem_process_seqs] Processed 687146 reads in 254.761 CPU sec, 8.084 real sec
sorting block 43, #reads = 1079554 mem = 536880932 peak = 980860928
[M::mem_process_seqs] Processed 685078 reads in 119.575 CPU sec, 3.809 real sec
sorting block 44, #reads = 1078948 mem = 536879332 peak = 980860928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 283565, 4, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (288, 288, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (94, 579)
[M::mem_pestat] mean and std.dev: (292.50, 76.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 676)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 315, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (331.99, 163.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 324, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (321.11, 179.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683508 sequences (100000031 bp) in 2.767 CPU sec, 2.950 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 283626, 9, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 269, 934)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2392)
[M::mem_pestat] mean and std.dev: (379.35, 294.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3121)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (336.74, 164.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 438, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (383.85, 155.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684836 reads in 169.653 CPU sec, 5.379 real sec
[M::process] read 683172 sequences (100000191 bp) in 1.890 CPU sec, 1.886 real sec...
[M::mem_process_seqs] Processed 683966 reads in 109.012 CPU sec, 3.439 real sec
sorting block 45, #reads = 1078428 mem = 536877531 peak = 980860928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 283492, 1, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 449, 2282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6392)
[M::mem_pestat] mean and std.dev: (871.94, 870.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8447)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 324, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (341.11, 167.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 266, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.00, 135.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682992 sequences (100000045 bp) in 2.429 CPU sec, 2.594 real sec...
[M::process] read 682958 sequences (100000003 bp) in 1.530 CPU sec, 1.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282831, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 278, 804)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2014)
[M::mem_pestat] mean and std.dev: (467.92, 355.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2619)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 324, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (341.73, 167.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (128, 222, 266)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542)
[M::mem_pestat] mean and std.dev: (204.29, 70.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 680)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683508 reads in 169.807 CPU sec, 5.397 real sec
[M::mem_process_seqs] Processed 683172 reads in 109.118 CPU sec, 3.462 real sec
sorting block 46, #reads = 1077147 mem = 536878885 peak = 980860928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 283309, 2, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 256, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (37, 432)
[M::mem_pestat] mean and std.dev: (228.67, 59.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 511)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 325, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (342.01, 167.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 320, 629)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1419)
[M::mem_pestat] mean and std.dev: (391.38, 241.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1814)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683360 sequences (100000120 bp) in 2.207 CPU sec, 2.331 real sec...
[M::process] read 692838 sequences (100000257 bp) in 1.970 CPU sec, 1.968 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 283449, 7, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 365, 1159)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3009)
[M::mem_pestat] mean and std.dev: (539.64, 421.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3934)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 325, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (343.63, 169.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 682992 reads in 172.821 CPU sec, 5.491 real sec
sorting block 47, #reads = 1077185 mem = 536880960 peak = 980860928
[M::mem_process_seqs] Processed 682958 reads in 110.345 CPU sec, 3.492 real sec
sorting block 48, #reads = 1075501 mem = 536876655 peak = 989810688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 283252, 4, 17)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 212, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (260.54, 102.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 323, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (342.36, 170.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 412, 734)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1784)
[M::mem_pestat] mean and std.dev: (414.80, 244.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2309)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688670 sequences (100000273 bp) in 2.798 CPU sec, 2.923 real sec...
[M::process] read 687588 sequences (100000074 bp) in 1.960 CPU sec, 1.964 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 287126, 4, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 237, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (269.18, 125.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 268, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (294.75, 149.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (307, 456, 538)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (383.83, 151.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683360 reads in 185.192 CPU sec, 5.864 real sec
[M::mem_process_seqs] Processed 692838 reads in 117.914 CPU sec, 3.733 real sec
sorting block 49, #reads = 1070767 mem = 536878723 peak = 989810688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 285381, 2, 19)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 183, 237)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439)
[M::mem_pestat] mean and std.dev: (172.12, 45.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 540)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 289, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (308.65, 151.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 171, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (218.53, 132.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 750)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686036 sequences (100000283 bp) in 2.166 CPU sec, 2.290 real sec...
[M::process] read 684532 sequences (100000002 bp) in 1.570 CPU sec, 1.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 284997, 5, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 211, 355)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (221.14, 77.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 874)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 298, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (317.94, 157.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 281, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (48, 583)
[M::mem_pestat] mean and std.dev: (294.04, 79.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 688670 reads in 183.761 CPU sec, 5.862 real sec
[M::mem_process_seqs] Processed 687588 reads in 119.047 CPU sec, 3.792 real sec
sorting block 50, #reads = 1074936 mem = 536877949 peak = 989810688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 284312, 8, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 254, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741)
[M::mem_pestat] mean and std.dev: (236.79, 86.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 305, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (323.31, 158.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 378, 804)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2040)
[M::mem_pestat] mean and std.dev: (477.72, 355.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2658)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684506 sequences (100000061 bp) in 2.634 CPU sec, 2.773 real sec...
[M::process] read 684060 sequences (100000065 bp) in 1.430 CPU sec, 1.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 283713, 7, 24)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 291, 727)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1843)
[M::mem_pestat] mean and std.dev: (444.12, 332.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2401)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 314, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (329.49, 158.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (280, 296, 784)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1792)
[M::mem_pestat] mean and std.dev: (387.95, 244.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2296)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686036 reads in 179.582 CPU sec, 5.715 real sec
[M::mem_process_seqs] Processed 684532 reads in 113.401 CPU sec, 3.609 real sec
sorting block 51, #reads = 1074897 mem = 536881479 peak = 989810688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 283928, 3, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 325, 647)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1493)
[M::mem_pestat] mean and std.dev: (313.88, 213.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1916)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 313, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (328.91, 158.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 272, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (325.44, 145.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 907)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683548 sequences (100000089 bp) in 2.485 CPU sec, 2.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 283075, 3, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 184, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (220.42, 91.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 315, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (331.59, 161.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 361, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (343.35, 169.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1058)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683190 sequences (100000195 bp) in 1.840 CPU sec, 1.838 real sec...
[M::mem_process_seqs] Processed 684506 reads in 176.855 CPU sec, 5.623 real sec
sorting block 52, #reads = 1073917 mem = 536878811 peak = 989810688
[M::mem_process_seqs] Processed 684060 reads in 111.394 CPU sec, 3.525 real sec
sorting block 53, #reads = 1074735 mem = 536880585 peak = 1021591552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 283356, 9, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 396, 1074)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2880)
[M::mem_pestat] mean and std.dev: (851.46, 879.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4368)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 319, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (336.73, 164.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 250, 409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (293.57, 118.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 644342 sequences (94434155 bp) in 2.688 CPU sec, 2.782 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 282674, 6, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (290, 340, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 710)
[M::mem_pestat] mean and std.dev: (322.50, 121.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 324, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (341.67, 167.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 269, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (295.67, 120.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683548 reads in 174.966 CPU sec, 5.604 real sec
[M::mem_process_seqs] Processed 683190 reads in 111.706 CPU sec, 3.593 real sec
sorting block 54, #reads = 1075775 mem = 536882677 peak = 1021591552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 267344, 2, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 363, 3786)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10942)
[M::mem_pestat] mean and std.dev: (1258.41, 1498.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14520)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 329, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (346.25, 169.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 299, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (306.25, 100.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 644342 reads in 87.087 CPU sec, 2.899 real sec
sorting block 55, #reads = 1073996 mem = 536877263 peak = 1023012864
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 60163032
overall: 69818892288 mem 10158.282 user 436.901 sys 373.920 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_3U6-T1-TDNA-1_A23MHWWLT4_1.L007\tPU:L007\tSM:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_3U6-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0002.1136_3U6-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 373.953 sec; CPU: 10595.186 sec
sorting block 56, #reads = 121766 mem = 61006658 peak = 1023012864
uniq header count 1
merging 57 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3612356020
output reads: 60485480
bam_mem_sort: 57 calls 32.808 user 1.184 sys 34.122 real
bam_write: 57 calls 16.369 user 1.287 sys 19.868 real
execute: 1 calls 17.557 user 0.740 sys 396.139 real
merge_files: 1 calls 17.537 user 0.711 sys 22.116 real
parse_chunk: 898 calls 39.619 user 9.871 sys 50.665 real
read_chunk: 2424 calls 19.485 user 17.037 sys 283.821 real
sort_block: 1 calls 13.297 user 16.481 sys 274.618 real
write_chunk: 1524 calls 17.195 user 0.541 sys 21.438 real
overall: 2714755072 mem 857.646 user 39.394 sys 396.161 real
ls: cannot access '*.cram': No such file or directory