This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_81F-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1451.872 CPU sec, 53.762 real sec [M::process] read 776276 sequences (100000109 bp) in 1.141 CPU sec, 1.145 real sec... [M::process] read 774324 sequences (100000003 bp) in 1.415 CPU sec, 1.443 real sec... [M::process] read 773676 sequences (100000147 bp) in 1.360 CPU sec, 1.352 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 296397, 36, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 425, 1138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3004) [M::mem_pestat] mean and std.dev: (576.94, 496.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 144, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (156.05, 63.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (226, 768, 1606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4366) [M::mem_pestat] mean and std.dev: (882.37, 751.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5746) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 328, 837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2107) [M::mem_pestat] mean and std.dev: (472.40, 433.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2742) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 772680 sequences (100000041 bp) in 1.360 CPU sec, 1.368 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 296557, 32, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 377, 858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2140) [M::mem_pestat] mean and std.dev: (482.26, 412.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (157.71, 64.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (238, 661, 2015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5569) [M::mem_pestat] mean and std.dev: (1158.75, 1175.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7346) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 243, 1117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3057) [M::mem_pestat] mean and std.dev: (505.52, 571.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4027) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 296329, 26, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 325, 1039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2727) [M::mem_pestat] mean and std.dev: (589.25, 550.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (157.35, 64.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (179, 677, 2271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6455) [M::mem_pestat] mean and std.dev: (1141.46, 1427.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 353, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1417) [M::mem_pestat] mean and std.dev: (370.79, 227.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 776276 reads in 305.416 CPU sec, 9.773 real sec [M::mem_process_seqs] Processed 773676 reads in 235.575 CPU sec, 7.626 real sec [M::mem_process_seqs] Processed 774324 reads in 267.538 CPU sec, 8.659 real sec sorting block 0, #reads = 1086110 mem = 536896151 peak = 605888512 [M::process] read 772698 sequences (100000217 bp) in 2.920 CPU sec, 3.135 real sec... sorting block 1, #reads = 1085683 mem = 536886100 peak = 819798016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 296028, 34, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 365, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2670) [M::mem_pestat] mean and std.dev: (561.58, 600.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (158.37, 65.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (204, 450, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2361) [M::mem_pestat] mean and std.dev: (487.54, 484.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3080) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 338, 963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2517) [M::mem_pestat] mean and std.dev: (496.08, 485.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3294) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 772262 sequences (100000073 bp) in 1.673 CPU sec, 1.701 real sec... [M::process] read 771438 sequences (100000049 bp) in 1.380 CPU sec, 1.381 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 295711, 43, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 468, 930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2440) [M::mem_pestat] mean and std.dev: (505.36, 405.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3195) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (158.39, 65.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (238, 892, 2867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8125) [M::mem_pestat] mean and std.dev: (1643.74, 1730.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10754) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 329, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2728) [M::mem_pestat] mean and std.dev: (552.79, 647.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 772680 reads in 211.131 CPU sec, 6.720 real sec [M::mem_process_seqs] Processed 772698 reads in 147.442 CPU sec, 4.695 real sec sorting block 2, #reads = 1086484 mem = 536880557 peak = 819798016 [M::process] read 770600 sequences (100000068 bp) in 2.861 CPU sec, 3.055 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 295473, 33, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 360, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2023) [M::mem_pestat] mean and std.dev: (500.84, 495.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.31, 64.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (162, 694, 1605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4491) [M::mem_pestat] mean and std.dev: (828.71, 851.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5934) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 382, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2152) [M::mem_pestat] mean and std.dev: (455.40, 392.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2797) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 770120 sequences (100000010 bp) in 2.024 CPU sec, 2.031 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 295835, 27, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 343, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1742) [M::mem_pestat] mean and std.dev: (381.59, 278.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2257) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.92, 65.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (186, 460, 916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2376) [M::mem_pestat] mean and std.dev: (476.17, 512.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3106) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 477, 1184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3076) [M::mem_pestat] mean and std.dev: (697.59, 680.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4022) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 772262 reads in 226.549 CPU sec, 7.192 real sec sorting block 3, #reads = 1085045 mem = 536886471 peak = 819798016 [M::mem_process_seqs] Processed 771438 reads in 269.231 CPU sec, 8.576 real sec sorting block 4, #reads = 1084812 mem = 536877605 peak = 819798016 [M::process] read 769966 sequences (100000021 bp) in 3.214 CPU sec, 3.356 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 294987, 21, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 289, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127) [M::mem_pestat] mean and std.dev: (304.38, 186.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.77, 65.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 383, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3583) [M::mem_pestat] mean and std.dev: (547.11, 521.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4709) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 344, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1979) [M::mem_pestat] mean and std.dev: (468.62, 407.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2574) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 769182 sequences (100000075 bp) in 2.180 CPU sec, 2.181 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 295287, 22, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 263, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169) [M::mem_pestat] mean and std.dev: (334.71, 222.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (159.34, 65.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (190, 709, 1165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3115) [M::mem_pestat] mean and std.dev: (608.19, 417.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4090) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 273, 798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2018) [M::mem_pestat] mean and std.dev: (375.48, 316.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 770120 reads in 232.056 CPU sec, 7.363 real sec [M::mem_process_seqs] Processed 770600 reads in 265.141 CPU sec, 8.438 real sec sorting block 5, #reads = 1084912 mem = 536880280 peak = 819798016 [M::process] read 769384 sequences (100000090 bp) in 2.563 CPU sec, 2.711 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 294251, 21, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 366, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3111) [M::mem_pestat] mean and std.dev: (659.31, 626.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.70, 65.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (263, 464, 757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1745) [M::mem_pestat] mean and std.dev: (428.33, 251.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 451, 1544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4148) [M::mem_pestat] mean and std.dev: (886.71, 968.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 768876 sequences (100000012 bp) in 2.190 CPU sec, 2.186 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 293878, 37, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 297, 634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1622) [M::mem_pestat] mean and std.dev: (385.24, 357.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2116) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.94, 65.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (223, 465, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2764) [M::mem_pestat] mean and std.dev: (555.62, 414.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3611) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 371, 772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1882) [M::mem_pestat] mean and std.dev: (459.88, 380.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2437) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 293667, 30, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 254, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2194) [M::mem_pestat] mean and std.dev: (469.33, 500.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.17, 66.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (172, 392, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2425) [M::mem_pestat] mean and std.dev: (471.07, 404.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3176) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 299, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319) [M::mem_pestat] mean and std.dev: (333.26, 219.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1692) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 769966 reads in 329.023 CPU sec, 10.518 real sec sorting block 6, #reads = 1083841 mem = 536879628 peak = 819798016 [M::mem_process_seqs] Processed 769384 reads in 262.632 CPU sec, 8.435 real sec [M::mem_process_seqs] Processed 769182 reads in 298.516 CPU sec, 9.582 real sec [M::process] read 768920 sequences (100000206 bp) in 3.116 CPU sec, 3.240 real sec... sorting block 7, #reads = 1082099 mem = 536879560 peak = 819798016 [M::process] read 768970 sequences (100000074 bp) in 3.272 CPU sec, 3.492 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 293842, 45, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 299, 774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2014) [M::mem_pestat] mean and std.dev: (486.30, 465.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.90, 65.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 450, 796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1966) [M::mem_pestat] mean and std.dev: (541.19, 436.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2551) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 328, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1966) [M::mem_pestat] mean and std.dev: (426.47, 361.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 768360 sequences (100000086 bp) in 2.220 CPU sec, 2.219 real sec... [M::mem_process_seqs] Processed 768920 reads in 143.711 CPU sec, 4.531 real sec sorting block 8, #reads = 1081641 mem = 536878534 peak = 819798016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 293765, 34, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 377, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1637) [M::mem_pestat] mean and std.dev: (438.43, 370.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.01, 65.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (111, 674, 1883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5427) [M::mem_pestat] mean and std.dev: (854.28, 1002.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7199) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 299, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1452) [M::mem_pestat] mean and std.dev: (343.72, 301.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 293846, 43, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 197, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (216.74, 136.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.09, 65.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (298, 623, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3547) [M::mem_pestat] mean and std.dev: (863.78, 842.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4630) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 555, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1886) [M::mem_pestat] mean and std.dev: (566.52, 434.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2447) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 768432 sequences (100000195 bp) in 2.633 CPU sec, 2.635 real sec... [M::mem_process_seqs] Processed 768876 reads in 382.693 CPU sec, 12.162 real sec [M::mem_process_seqs] Processed 768970 reads in 209.977 CPU sec, 6.674 real sec sorting block 9, #reads = 1080265 mem = 536886589 peak = 819798016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 293994, 40, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 293, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (294.61, 181.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1257) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.16, 65.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 638, 2022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5584) [M::mem_pestat] mean and std.dev: (1212.32, 1565.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7473) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 204, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1401) [M::mem_pestat] mean and std.dev: (351.62, 297.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 768320 sequences (100000040 bp) in 3.474 CPU sec, 3.725 real sec... [M::process] read 766140 sequences (100000254 bp) in 2.270 CPU sec, 2.271 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 294086, 31, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 304, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2701) [M::mem_pestat] mean and std.dev: (517.71, 504.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.38, 65.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (464, 1015, 2693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7151) [M::mem_pestat] mean and std.dev: (1337.69, 1118.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 245, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2203) [M::mem_pestat] mean and std.dev: (344.68, 333.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2887) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 768360 reads in 243.524 CPU sec, 7.733 real sec sorting block 10, #reads = 1082478 mem = 536879224 peak = 819798016 [M::mem_process_seqs] Processed 768432 reads in 167.169 CPU sec, 5.571 real sec sorting block 11, #reads = 1081149 mem = 536883827 peak = 852516864 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 293860, 37, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 255, 670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1708) [M::mem_pestat] mean and std.dev: (396.30, 352.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2227) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.61, 66.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (148, 580, 1446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4042) [M::mem_pestat] mean and std.dev: (716.79, 752.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 384, 995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2695) [M::mem_pestat] mean and std.dev: (640.75, 655.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 767128 sequences (100000031 bp) in 3.220 CPU sec, 3.450 real sec... [M::process] read 766922 sequences (100000203 bp) in 2.370 CPU sec, 2.365 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 293055, 42, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 417, 1057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2775) [M::mem_pestat] mean and std.dev: (538.62, 473.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 149, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (161.96, 66.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (114, 453, 2057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5943) [M::mem_pestat] mean and std.dev: (1013.55, 1242.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7886) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 273, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2133) [M::mem_pestat] mean and std.dev: (408.06, 341.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 768320 reads in 247.497 CPU sec, 8.111 real sec [M::process] read 766488 sequences (100000157 bp) in 2.472 CPU sec, 2.473 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 293680, 34, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 281, 1425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3945) [M::mem_pestat] mean and std.dev: (748.74, 954.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5205) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 149, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.47, 66.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (290, 541, 1715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4565) [M::mem_pestat] mean and std.dev: (936.16, 914.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5990) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 293, 1209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3319) [M::mem_pestat] mean and std.dev: (633.77, 684.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4374) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 767128 reads in 151.602 CPU sec, 5.050 real sec sorting block 12, #reads = 1082885 mem = 536880142 peak = 852516864 [M::mem_process_seqs] Processed 766140 reads in 362.501 CPU sec, 11.798 real sec sorting block 13, #reads = 1080537 mem = 536880537 peak = 961122304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 293602, 37, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 256, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1536) [M::mem_pestat] mean and std.dev: (401.11, 367.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 149, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (161.78, 67.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (440, 842, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3713) [M::mem_pestat] mean and std.dev: (938.37, 735.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 435, 1010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2586) [M::mem_pestat] mean and std.dev: (646.18, 568.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3374) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 767234 sequences (100000165 bp) in 3.193 CPU sec, 3.709 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 293449, 53, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 466, 1462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4036) [M::mem_pestat] mean and std.dev: (713.69, 803.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (112, 149, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (162.05, 67.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (207, 477, 1985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5541) [M::mem_pestat] mean and std.dev: (981.60, 1163.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7319) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 243, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1891) [M::mem_pestat] mean and std.dev: (419.36, 379.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2463) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 766516 sequences (100000048 bp) in 2.338 CPU sec, 2.337 real sec... [M::mem_process_seqs] Processed 766922 reads in 271.898 CPU sec, 8.641 real sec sorting block 14, #reads = 1081930 mem = 536882712 peak = 961122304 [M::mem_process_seqs] Processed 766488 reads in 165.352 CPU sec, 5.467 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 293734, 33, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 336, 800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2094) [M::mem_pestat] mean and std.dev: (471.20, 447.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 149, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (161.90, 67.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (426, 923, 2157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5619) [M::mem_pestat] mean and std.dev: (1233.77, 1272.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 263, 915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2363) [M::mem_pestat] mean and std.dev: (541.72, 565.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3087) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 766640 sequences (100000218 bp) in 3.096 CPU sec, 3.283 real sec... [M::process] read 773704 sequences (100000249 bp) in 1.780 CPU sec, 1.775 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 293164, 31, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 266, 1379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3819) [M::mem_pestat] mean and std.dev: (723.57, 885.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 149, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (162.22, 67.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (408, 721, 1838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4698) [M::mem_pestat] mean and std.dev: (807.27, 833.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6128) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 284, 672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1698) [M::mem_pestat] mean and std.dev: (372.80, 309.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2211) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 767234 reads in 235.304 CPU sec, 7.715 real sec sorting block 15, #reads = 1080054 mem = 536881625 peak = 961122304 [M::mem_process_seqs] Processed 766516 reads in 161.728 CPU sec, 5.175 real sec sorting block 16, #reads = 1083309 mem = 536879711 peak = 961122304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 293315, 48, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 277, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2063) [M::mem_pestat] mean and std.dev: (476.56, 440.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 149, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (162.06, 67.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (176, 588, 2160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6128) [M::mem_pestat] mean and std.dev: (964.04, 1057.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8112) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 250, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1653) [M::mem_pestat] mean and std.dev: (412.04, 364.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 774868 sequences (100000029 bp) in 3.337 CPU sec, 3.539 real sec... [M::process] read 773528 sequences (100000126 bp) in 1.890 CPU sec, 1.894 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 296017, 33, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 266, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247) [M::mem_pestat] mean and std.dev: (322.08, 269.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (157.15, 63.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (184, 863, 2441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6955) [M::mem_pestat] mean and std.dev: (1197.97, 1078.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9212) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 275, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (251.12, 136.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 766640 reads in 233.232 CPU sec, 7.624 real sec [M::mem_process_seqs] Processed 773704 reads in 165.698 CPU sec, 5.301 real sec sorting block 17, #reads = 1079402 mem = 536882273 peak = 961122304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 297090, 44, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 280, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1780) [M::mem_pestat] mean and std.dev: (404.82, 342.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2311) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (156.83, 64.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (90, 347, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2718) [M::mem_pestat] mean and std.dev: (493.39, 472.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3594) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 274, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1634) [M::mem_pestat] mean and std.dev: (390.03, 334.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2120) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 773262 sequences (100000181 bp) in 3.141 CPU sec, 3.273 real sec... [M::process] read 772730 sequences (100000073 bp) in 2.350 CPU sec, 2.352 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 296161, 42, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 361, 1028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2708) [M::mem_pestat] mean and std.dev: (546.75, 540.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (157.57, 64.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (504, 1047, 2036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5100) [M::mem_pestat] mean and std.dev: (1286.38, 1000.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6632) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 282, 1216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3348) [M::mem_pestat] mean and std.dev: (579.55, 615.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4414) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 774868 reads in 232.776 CPU sec, 7.418 real sec sorting block 18, #reads = 1084792 mem = 536879169 peak = 961122304 [M::mem_process_seqs] Processed 773528 reads in 164.071 CPU sec, 5.312 real sec sorting block 19, #reads = 1088530 mem = 536880671 peak = 961122304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 296192, 50, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 441, 1894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5352) [M::mem_pestat] mean and std.dev: (1017.19, 1257.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (157.77, 64.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (244, 537, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1621) [M::mem_pestat] mean and std.dev: (538.51, 419.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2215) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 332, 1108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2908) [M::mem_pestat] mean and std.dev: (725.12, 768.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 772372 sequences (100000126 bp) in 2.916 CPU sec, 3.006 real sec... [M::process] read 771488 sequences (100000081 bp) in 1.770 CPU sec, 1.771 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 296159, 38, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 647, 1494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4134) [M::mem_pestat] mean and std.dev: (744.14, 698.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (158.69, 65.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 747, 1767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4937) [M::mem_pestat] mean and std.dev: (1013.97, 968.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 337, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1999) [M::mem_pestat] mean and std.dev: (522.66, 482.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 773262 reads in 229.067 CPU sec, 7.368 real sec [M::mem_process_seqs] Processed 772730 reads in 161.083 CPU sec, 5.161 real sec sorting block 20, #reads = 1087988 mem = 536879294 peak = 961122304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 296183, 29, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 278, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1262) [M::mem_pestat] mean and std.dev: (340.20, 242.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.38, 64.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (110, 330, 714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1922) [M::mem_pestat] mean and std.dev: (365.73, 366.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 306, 919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2383) [M::mem_pestat] mean and std.dev: (505.29, 475.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3115) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 771380 sequences (100000286 bp) in 3.054 CPU sec, 3.246 real sec... [M::process] read 770660 sequences (100000154 bp) in 1.840 CPU sec, 1.839 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 295651, 30, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 214, 960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2562) [M::mem_pestat] mean and std.dev: (460.66, 471.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.69, 65.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (140, 690, 1613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4559) [M::mem_pestat] mean and std.dev: (901.37, 988.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6032) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 479, 1096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2924) [M::mem_pestat] mean and std.dev: (614.78, 596.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 772372 reads in 224.991 CPU sec, 7.185 real sec sorting block 21, #reads = 1086433 mem = 536883849 peak = 961122304 [M::mem_process_seqs] Processed 771488 reads in 163.406 CPU sec, 5.374 real sec sorting block 22, #reads = 1086555 mem = 536877935 peak = 961122304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 295921, 43, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 335, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1775) [M::mem_pestat] mean and std.dev: (375.80, 322.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (159.09, 65.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 786, 1878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5224) [M::mem_pestat] mean and std.dev: (946.07, 917.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6897) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 386, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2123) [M::mem_pestat] mean and std.dev: (458.94, 312.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2761) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 770002 sequences (100000104 bp) in 3.815 CPU sec, 3.994 real sec... [M::process] read 770344 sequences (100000190 bp) in 2.330 CPU sec, 2.326 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 295226, 47, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 326, 1212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3298) [M::mem_pestat] mean and std.dev: (700.71, 796.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.88, 65.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (227, 701, 1835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5051) [M::mem_pestat] mean and std.dev: (1077.42, 1133.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 281, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2065) [M::mem_pestat] mean and std.dev: (391.35, 347.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2699) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 771380 reads in 227.162 CPU sec, 7.338 real sec [M::mem_process_seqs] Processed 770660 reads in 254.739 CPU sec, 8.557 real sec sorting block 23, #reads = 1087727 mem = 536879475 peak = 961122304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 295002, 51, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 231, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1587) [M::mem_pestat] mean and std.dev: (317.34, 262.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.69, 65.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (301, 621, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2476) [M::mem_pestat] mean and std.dev: (658.27, 473.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3201) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 322, 1455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3955) [M::mem_pestat] mean and std.dev: (538.78, 576.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5205) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 769494 sequences (100000084 bp) in 3.263 CPU sec, 3.417 real sec... [M::process] read 769678 sequences (100000060 bp) in 2.340 CPU sec, 2.342 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 294376, 33, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 196, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1842) [M::mem_pestat] mean and std.dev: (384.22, 367.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.67, 65.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (156, 225, 1319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3645) [M::mem_pestat] mean and std.dev: (645.35, 813.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4808) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 447, 1672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4510) [M::mem_pestat] mean and std.dev: (963.81, 1089.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 770002 reads in 237.186 CPU sec, 7.844 real sec sorting block 24, #reads = 1085736 mem = 536882222 peak = 961122304 [M::mem_process_seqs] Processed 770344 reads in 170.713 CPU sec, 5.452 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 294072, 49, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 340, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1526) [M::mem_pestat] mean and std.dev: (379.36, 339.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.62, 65.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (152, 424, 950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2546) [M::mem_pestat] mean and std.dev: (520.36, 462.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 315, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2527) [M::mem_pestat] mean and std.dev: (552.93, 548.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3296) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 769330 sequences (100000038 bp) in 3.077 CPU sec, 3.215 real sec... [M::process] read 769238 sequences (100000246 bp) in 2.290 CPU sec, 2.291 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 293996, 44, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 318, 1049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2813) [M::mem_pestat] mean and std.dev: (569.28, 612.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (159.24, 65.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 476, 952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2492) [M::mem_pestat] mean and std.dev: (560.60, 557.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3262) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 358, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3043) [M::mem_pestat] mean and std.dev: (554.42, 545.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4000) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 769494 reads in 231.837 CPU sec, 7.391 real sec sorting block 25, #reads = 1084052 mem = 536881851 peak = 961122304 [M::mem_process_seqs] Processed 769678 reads in 176.451 CPU sec, 5.645 real sec sorting block 26, #reads = 1082085 mem = 536886949 peak = 1026195456 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 293719, 50, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 357, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2986) [M::mem_pestat] mean and std.dev: (599.55, 625.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.26, 64.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (392, 677, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3311) [M::mem_pestat] mean and std.dev: (757.80, 626.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4284) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 649, 1726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4712) [M::mem_pestat] mean and std.dev: (891.74, 909.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6205) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 769394 sequences (100000220 bp) in 3.568 CPU sec, 3.731 real sec... [M::process] read 768376 sequences (100000240 bp) in 2.400 CPU sec, 2.398 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 293875, 40, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 280, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1647) [M::mem_pestat] mean and std.dev: (375.35, 343.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2142) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.64, 65.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (458, 773, 1532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3680) [M::mem_pestat] mean and std.dev: (916.91, 861.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4754) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 273, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1975) [M::mem_pestat] mean and std.dev: (467.88, 512.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 769330 reads in 246.037 CPU sec, 7.798 real sec sorting block 27, #reads = 1082734 mem = 536880719 peak = 1026195456 [M::mem_process_seqs] Processed 769238 reads in 286.341 CPU sec, 9.104 real sec [M::process] read 768486 sequences (100000289 bp) in 3.812 CPU sec, 3.967 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 293932, 45, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 233, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2789) [M::mem_pestat] mean and std.dev: (527.38, 638.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.87, 65.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (258, 589, 1067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2685) [M::mem_pestat] mean and std.dev: (648.51, 569.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3494) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 322, 1061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2801) [M::mem_pestat] mean and std.dev: (586.65, 654.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3671) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 769022 sequences (100000173 bp) in 2.220 CPU sec, 2.214 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 293896, 39, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 432, 899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2305) [M::mem_pestat] mean and std.dev: (539.27, 453.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.12, 65.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 736, 2232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6196) [M::mem_pestat] mean and std.dev: (1224.03, 1409.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8178) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 308, 1323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3591) [M::mem_pestat] mean and std.dev: (620.25, 657.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4725) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 769394 reads in 259.576 CPU sec, 8.657 real sec sorting block 28, #reads = 1081525 mem = 536879318 peak = 1026195456 [M::mem_process_seqs] Processed 768376 reads in 298.180 CPU sec, 9.921 real sec sorting block 29, #reads = 1084043 mem = 536875878 peak = 1026195456 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 294045, 46, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 346, 1259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3409) [M::mem_pestat] mean and std.dev: (666.86, 754.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.13, 65.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (364, 594, 982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2218) [M::mem_pestat] mean and std.dev: (609.98, 383.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2836) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 299, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1305) [M::mem_pestat] mean and std.dev: (367.20, 280.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1677) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 768588 sequences (100000084 bp) in 3.436 CPU sec, 3.665 real sec... [M::process] read 768362 sequences (100000020 bp) in 2.460 CPU sec, 2.455 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 293927, 44, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 341, 849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2223) [M::mem_pestat] mean and std.dev: (473.90, 494.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (160.15, 66.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (90, 354, 799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2217) [M::mem_pestat] mean and std.dev: (466.48, 479.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2926) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 313, 778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1990) [M::mem_pestat] mean and std.dev: (425.89, 393.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2596) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 768486 reads in 253.017 CPU sec, 8.063 real sec [M::mem_process_seqs] Processed 769022 reads in 193.076 CPU sec, 6.201 real sec sorting block 30, #reads = 1082274 mem = 536878139 peak = 1026195456 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 294442, 37, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 281, 911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2421) [M::mem_pestat] mean and std.dev: (514.09, 555.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.36, 66.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (73, 230, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2008) [M::mem_pestat] mean and std.dev: (373.66, 407.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 296, 761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1991) [M::mem_pestat] mean and std.dev: (431.31, 440.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2606) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 768438 sequences (100000002 bp) in 3.778 CPU sec, 3.969 real sec... [M::process] read 767828 sequences (100000099 bp) in 1.910 CPU sec, 1.904 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 294010, 31, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 331, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2188) [M::mem_pestat] mean and std.dev: (489.51, 412.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2856) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.38, 65.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (261, 756, 1375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3603) [M::mem_pestat] mean and std.dev: (851.38, 797.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 423, 1105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2917) [M::mem_pestat] mean and std.dev: (600.67, 556.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 768588 reads in 249.632 CPU sec, 7.943 real sec sorting block 31, #reads = 1082479 mem = 536877613 peak = 1026195456 [M::mem_process_seqs] Processed 768362 reads in 291.675 CPU sec, 9.313 real sec sorting block 32, #reads = 1081951 mem = 536880089 peak = 1026195456 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 293854, 22, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 292, 994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2718) [M::mem_pestat] mean and std.dev: (526.84, 606.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.00, 66.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (154, 430, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1552) [M::mem_pestat] mean and std.dev: (392.00, 234.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2018) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 293, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1492) [M::mem_pestat] mean and std.dev: (336.86, 280.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1933) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 767380 sequences (100000218 bp) in 3.343 CPU sec, 3.475 real sec... [M::process] read 767892 sequences (100000045 bp) in 2.570 CPU sec, 2.564 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 294040, 37, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 221, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1439) [M::mem_pestat] mean and std.dev: (340.82, 299.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.12, 66.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 516, 762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1938) [M::mem_pestat] mean and std.dev: (459.47, 339.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 329, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2135) [M::mem_pestat] mean and std.dev: (492.21, 504.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 768438 reads in 244.334 CPU sec, 7.751 real sec [M::mem_process_seqs] Processed 767828 reads in 176.078 CPU sec, 5.601 real sec sorting block 33, #reads = 1082516 mem = 536884451 peak = 1026195456 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 293551, 43, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 224, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282) [M::mem_pestat] mean and std.dev: (326.92, 296.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 149, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (161.52, 66.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (160, 463, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2575) [M::mem_pestat] mean and std.dev: (570.50, 573.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (310, 485, 969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2287) [M::mem_pestat] mean and std.dev: (562.11, 442.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2946) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 773664 sequences (100000141 bp) in 3.415 CPU sec, 3.555 real sec... [M::process] read 774914 sequences (100000248 bp) in 2.530 CPU sec, 2.524 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 293524, 37, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 319, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1272) [M::mem_pestat] mean and std.dev: (349.44, 228.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 149, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.35, 66.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (237, 639, 1357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3597) [M::mem_pestat] mean and std.dev: (717.35, 609.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 366, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1693) [M::mem_pestat] mean and std.dev: (425.24, 367.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2202) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 767380 reads in 247.740 CPU sec, 7.864 real sec sorting block 34, #reads = 1082024 mem = 536877687 peak = 1026195456 [M::mem_process_seqs] Processed 767892 reads in 180.420 CPU sec, 5.747 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 295908, 39, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 330, 1121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3047) [M::mem_pestat] mean and std.dev: (627.63, 713.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (157.47, 64.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (230, 506, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2267) [M::mem_pestat] mean and std.dev: (617.73, 570.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2946) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 240, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184) [M::mem_pestat] mean and std.dev: (322.15, 265.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 35, #reads = 1081001 mem = 536875289 peak = 1026195456 [M::process] read 773586 sequences (100000051 bp) in 3.523 CPU sec, 3.679 real sec... [M::process] read 773428 sequences (100000282 bp) in 2.530 CPU sec, 2.527 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 296387, 29, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 393, 957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2437) [M::mem_pestat] mean and std.dev: (488.98, 454.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (156.78, 63.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (233, 487, 2491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7007) [M::mem_pestat] mean and std.dev: (1251.41, 1775.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9265) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 421, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2208) [M::mem_pestat] mean and std.dev: (495.39, 384.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2882) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 773664 reads in 254.184 CPU sec, 8.069 real sec [M::mem_process_seqs] Processed 774914 reads in 182.841 CPU sec, 5.821 real sec sorting block 36, #reads = 1085906 mem = 536879004 peak = 1026195456 [M::process] read 771940 sequences (100000014 bp) in 3.020 CPU sec, 3.201 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 296569, 36, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 205, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1493) [M::mem_pestat] mean and std.dev: (276.67, 211.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (157.74, 64.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 707, 1229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3175) [M::mem_pestat] mean and std.dev: (656.76, 488.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4148) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 307, 804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2000) [M::mem_pestat] mean and std.dev: (465.55, 402.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 771518 sequences (100000111 bp) in 2.269 CPU sec, 2.275 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 296820, 43, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 275, 829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2145) [M::mem_pestat] mean and std.dev: (389.91, 399.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (158.14, 64.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (254, 392, 1868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5096) [M::mem_pestat] mean and std.dev: (798.68, 951.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 513, 1710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4862) [M::mem_pestat] mean and std.dev: (1048.73, 1149.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6438) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 773586 reads in 253.847 CPU sec, 8.182 real sec [M::mem_process_seqs] Processed 773428 reads in 177.886 CPU sec, 5.697 real sec sorting block 37, #reads = 1086921 mem = 536878372 peak = 1026195456 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 295681, 35, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 272, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2436) [M::mem_pestat] mean and std.dev: (491.41, 558.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3194) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.57, 65.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (246, 533, 969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2415) [M::mem_pestat] mean and std.dev: (615.58, 533.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3138) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 340, 1161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3127) [M::mem_pestat] mean and std.dev: (554.58, 588.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4110) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 770960 sequences (100000177 bp) in 3.363 CPU sec, 3.485 real sec... [M::process] read 770716 sequences (100000119 bp) in 2.100 CPU sec, 2.101 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 295930, 38, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 272, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2109) [M::mem_pestat] mean and std.dev: (384.08, 349.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.90, 65.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (412, 723, 900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1876) [M::mem_pestat] mean and std.dev: (676.40, 372.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 423, 885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2289) [M::mem_pestat] mean and std.dev: (487.02, 408.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2991) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 771940 reads in 253.217 CPU sec, 8.097 real sec sorting block 38, #reads = 1086771 mem = 536882136 peak = 1026195456 [M::mem_process_seqs] Processed 771518 reads in 177.651 CPU sec, 5.668 real sec sorting block 39, #reads = 1087894 mem = 536881970 peak = 1026195456 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 295328, 36, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 261, 652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1672) [M::mem_pestat] mean and std.dev: (392.10, 333.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 770502 sequences (100000131 bp) in 3.036 CPU sec, 3.182 real sec... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.62, 65.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (144, 815, 1187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3273) [M::mem_pestat] mean and std.dev: (670.77, 628.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 305, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1677) [M::mem_pestat] mean and std.dev: (417.65, 404.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2180) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 770960 reads in 145.198 CPU sec, 4.768 real sec [M::process] read 769626 sequences (100000041 bp) in 2.845 CPU sec, 3.048 real sec... sorting block 40, #reads = 1085143 mem = 536881826 peak = 1026195456 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 294705, 45, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 280, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1455) [M::mem_pestat] mean and std.dev: (371.91, 301.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.40, 65.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (391, 792, 2622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7084) [M::mem_pestat] mean and std.dev: (1727.07, 2130.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10250) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 403, 1493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4093) [M::mem_pestat] mean and std.dev: (689.24, 752.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 769626 sequences (100000270 bp) in 2.557 CPU sec, 2.560 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 294700, 42, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 207, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1382) [M::mem_pestat] mean and std.dev: (318.95, 268.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.78, 65.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (168, 553, 1442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3990) [M::mem_pestat] mean and std.dev: (977.37, 1132.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5506) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 313, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (282.48, 121.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 295584, 24, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 344, 890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2346) [M::mem_pestat] mean and std.dev: (508.91, 485.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.82, 66.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (194, 596, 1666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4610) [M::mem_pestat] mean and std.dev: (770.14, 811.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6082) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 371, 1062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2768) [M::mem_pestat] mean and std.dev: (576.95, 541.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 770716 reads in 376.707 CPU sec, 12.066 real sec [M::mem_process_seqs] Processed 769626 reads in 268.885 CPU sec, 8.632 real sec [M::mem_process_seqs] Processed 770502 reads in 304.459 CPU sec, 9.786 real sec sorting block 41, #reads = 1085329 mem = 536880116 peak = 1026195456 [M::process] read 769922 sequences (100000017 bp) in 3.543 CPU sec, 3.716 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 294749, 39, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 242, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1274) [M::mem_pestat] mean and std.dev: (293.71, 238.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (160.00, 66.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (259, 710, 1169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2989) [M::mem_pestat] mean and std.dev: (732.90, 565.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3899) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 330, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2105) [M::mem_pestat] mean and std.dev: (407.33, 347.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 42, #reads = 1083794 mem = 536882802 peak = 1026195456 [M::process] read 768968 sequences (100000090 bp) in 3.443 CPU sec, 3.745 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 294905, 41, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 256, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043) [M::mem_pestat] mean and std.dev: (304.88, 194.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.89, 65.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (331, 677, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2887) [M::mem_pestat] mean and std.dev: (756.06, 630.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3739) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 255, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2123) [M::mem_pestat] mean and std.dev: (595.92, 603.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3009) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 769626 reads in 245.723 CPU sec, 7.844 real sec [M::mem_process_seqs] Processed 769922 reads in 178.097 CPU sec, 5.623 real sec [M::process] read 768354 sequences (100000070 bp) in 2.657 CPU sec, 2.797 real sec... sorting block 43, #reads = 1084611 mem = 536881558 peak = 1026195456 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 293694, 33, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 214, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2069) [M::mem_pestat] mean and std.dev: (401.46, 441.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.09, 65.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 477, 1079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2889) [M::mem_pestat] mean and std.dev: (515.00, 463.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3794) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 341, 1258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3440) [M::mem_pestat] mean and std.dev: (557.81, 549.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4531) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 768524 sequences (100000077 bp) in 3.562 CPU sec, 3.764 real sec... [M::process] read 768506 sequences (100000156 bp) in 2.380 CPU sec, 2.371 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 293949, 35, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 275, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1528) [M::mem_pestat] mean and std.dev: (382.49, 291.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.43, 65.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (382, 687, 1759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4513) [M::mem_pestat] mean and std.dev: (1003.97, 905.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5890) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 299, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1192) [M::mem_pestat] mean and std.dev: (305.10, 204.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 768968 reads in 263.321 CPU sec, 8.408 real sec sorting block 44, #reads = 1083192 mem = 536879171 peak = 1026195456 [M::mem_process_seqs] Processed 768354 reads in 183.010 CPU sec, 6.125 real sec sorting block 45, #reads = 1080058 mem = 536890521 peak = 1026195456 [M::process] read 768410 sequences (100000021 bp) in 3.085 CPU sec, 3.185 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 293673, 26, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 552, 1317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3587) [M::mem_pestat] mean and std.dev: (724.50, 704.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.39, 65.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (142, 532, 1000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2716) [M::mem_pestat] mean and std.dev: (481.09, 478.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3574) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 306, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1217) [M::mem_pestat] mean and std.dev: (315.04, 210.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1553) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 768744 sequences (100000230 bp) in 2.398 CPU sec, 2.401 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 293790, 37, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 269, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2994) [M::mem_pestat] mean and std.dev: (572.78, 611.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.30, 65.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 497, 1553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4405) [M::mem_pestat] mean and std.dev: (1078.57, 1212.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5927) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 340, 946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2502) [M::mem_pestat] mean and std.dev: (520.85, 479.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3280) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 293528, 39, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 451, 1089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2873) [M::mem_pestat] mean and std.dev: (655.50, 609.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.37, 65.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 464, 1147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3297) [M::mem_pestat] mean and std.dev: (583.72, 706.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4372) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 368, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2628) [M::mem_pestat] mean and std.dev: (498.88, 525.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3448) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 768524 reads in 361.372 CPU sec, 11.820 real sec [M::mem_process_seqs] Processed 768506 reads in 286.934 CPU sec, 9.217 real sec [M::mem_process_seqs] Processed 768410 reads in 327.984 CPU sec, 10.552 real sec sorting block 46, #reads = 1080724 mem = 536879122 peak = 1026195456 [M::process] read 768510 sequences (100000066 bp) in 3.372 CPU sec, 3.496 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 294017, 25, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 261, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1554) [M::mem_pestat] mean and std.dev: (374.27, 327.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.30, 65.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (499, 909, 1918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4756) [M::mem_pestat] mean and std.dev: (1106.61, 941.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6175) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 367, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803) [M::mem_pestat] mean and std.dev: (424.54, 307.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2335) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 47, #reads = 1082892 mem = 536881668 peak = 1026195456 [M::process] read 768354 sequences (100000248 bp) in 3.081 CPU sec, 3.246 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 293811, 35, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 463, 1269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3415) [M::mem_pestat] mean and std.dev: (651.30, 615.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.33, 65.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (224, 504, 1345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3587) [M::mem_pestat] mean and std.dev: (775.32, 736.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4708) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 389, 1021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2623) [M::mem_pestat] mean and std.dev: (545.23, 489.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3424) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 768368 sequences (100000234 bp) in 2.157 CPU sec, 2.165 real sec... [M::mem_process_seqs] Processed 768744 reads in 263.004 CPU sec, 8.393 real sec [M::mem_process_seqs] Processed 768510 reads in 189.574 CPU sec, 6.031 real sec sorting block 48, #reads = 1083245 mem = 536883296 peak = 1026195456 [M::process] read 767904 sequences (100000082 bp) in 2.929 CPU sec, 3.079 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 293502, 36, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 301, 1315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3599) [M::mem_pestat] mean and std.dev: (560.22, 695.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.48, 65.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (196, 446, 1669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4615) [M::mem_pestat] mean and std.dev: (768.82, 857.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6088) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 309, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1308) [M::mem_pestat] mean and std.dev: (361.64, 210.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1673) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 767496 sequences (100000094 bp) in 1.835 CPU sec, 1.839 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 293903, 42, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 279, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2363) [M::mem_pestat] mean and std.dev: (517.31, 562.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3101) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.55, 66.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 814, 2627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7533) [M::mem_pestat] mean and std.dev: (1626.37, 1902.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9986) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 486, 1741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4813) [M::mem_pestat] mean and std.dev: (917.44, 1045.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6349) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 768354 reads in 255.041 CPU sec, 8.613 real sec sorting block 49, #reads = 1082397 mem = 536879611 peak = 1026195456 [M::mem_process_seqs] Processed 768368 reads in 185.443 CPU sec, 5.966 real sec sorting block 50, #reads = 1081376 mem = 536882637 peak = 1026195456 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 293944, 19, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 393, 1179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3157) [M::mem_pestat] mean and std.dev: (542.72, 564.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4146) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.13, 66.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (221, 793, 1662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4544) [M::mem_pestat] mean and std.dev: (813.50, 823.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5985) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 314, 1377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3841) [M::mem_pestat] mean and std.dev: (687.52, 889.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5073) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 766858 sequences (100000067 bp) in 3.668 CPU sec, 4.224 real sec... [M::process] read 766712 sequences (100000206 bp) in 2.440 CPU sec, 2.441 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 293798, 35, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 332, 1061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2803) [M::mem_pestat] mean and std.dev: (560.95, 623.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.53, 65.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (134, 721, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3566) [M::mem_pestat] mean and std.dev: (655.61, 533.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 488, 1117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2995) [M::mem_pestat] mean and std.dev: (602.44, 619.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3934) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 767496 reads in 256.034 CPU sec, 8.125 real sec [M::mem_process_seqs] Processed 767904 reads in 295.869 CPU sec, 9.952 real sec sorting block 51, #reads = 1081045 mem = 536877199 peak = 1026195456 [M::process] read 774106 sequences (100000056 bp) in 3.960 CPU sec, 4.167 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 293223, 39, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 371, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1931) [M::mem_pestat] mean and std.dev: (408.37, 333.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 149, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (162.02, 67.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (310, 802, 1836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4888) [M::mem_pestat] mean and std.dev: (1034.33, 1156.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 357, 885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2211) [M::mem_pestat] mean and std.dev: (516.12, 431.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2874) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 775424 sequences (100000143 bp) in 2.510 CPU sec, 2.509 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 293457, 35, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 273, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2634) [M::mem_pestat] mean and std.dev: (456.07, 517.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 149, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.59, 66.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (163, 500, 1747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4915) [M::mem_pestat] mean and std.dev: (926.62, 1186.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6499) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 612, 1959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5465) [M::mem_pestat] mean and std.dev: (985.15, 997.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7218) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 766712 reads in 264.875 CPU sec, 8.922 real sec sorting block 52, #reads = 1082217 mem = 536887256 peak = 1026195456 [M::mem_process_seqs] Processed 766858 reads in 296.631 CPU sec, 10.070 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 296648, 44, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 268, 731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1877) [M::mem_pestat] mean and std.dev: (385.00, 337.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (157.40, 64.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (394, 733, 1442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3538) [M::mem_pestat] mean and std.dev: (906.60, 752.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4586) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 300, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1365) [M::mem_pestat] mean and std.dev: (323.98, 216.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1761) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 53, #reads = 1080345 mem = 536883328 peak = 1026195456 [M::process] read 774016 sequences (100000292 bp) in 3.813 CPU sec, 4.065 real sec... [M::process] read 773448 sequences (100000091 bp) in 2.300 CPU sec, 2.294 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 296579, 30, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 303, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3184) [M::mem_pestat] mean and std.dev: (514.94, 484.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (156.29, 63.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (77, 528, 1372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3962) [M::mem_pestat] mean and std.dev: (626.86, 648.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 408, 1489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4129) [M::mem_pestat] mean and std.dev: (714.75, 695.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5449) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 774106 reads in 253.002 CPU sec, 8.143 real sec [M::mem_process_seqs] Processed 775424 reads in 180.928 CPU sec, 5.760 real sec sorting block 54, #reads = 1085990 mem = 536880636 peak = 1026195456 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 296736, 26, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 237, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1823) [M::mem_pestat] mean and std.dev: (417.05, 457.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (157.32, 64.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (190, 513, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2347) [M::mem_pestat] mean and std.dev: (389.40, 284.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3066) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 294, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3015) [M::mem_pestat] mean and std.dev: (555.20, 542.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 773262 sequences (100000283 bp) in 3.529 CPU sec, 3.719 real sec... [M::process] read 772622 sequences (100000283 bp) in 2.410 CPU sec, 2.410 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 296186, 39, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 273, 820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2178) [M::mem_pestat] mean and std.dev: (431.79, 434.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (157.57, 64.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (402, 752, 2581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6939) [M::mem_pestat] mean and std.dev: (1849.72, 2227.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10760) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 348, 1417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3863) [M::mem_pestat] mean and std.dev: (678.61, 715.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5086) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 774016 reads in 251.050 CPU sec, 8.183 real sec sorting block 55, #reads = 1089252 mem = 536879327 peak = 1026195456 [M::mem_process_seqs] Processed 773448 reads in 175.352 CPU sec, 5.588 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 295907, 32, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 538, 1095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2941) [M::mem_pestat] mean and std.dev: (744.12, 757.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (158.03, 64.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (247, 787, 1671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4519) [M::mem_pestat] mean and std.dev: (955.72, 923.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5943) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 360, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2019) [M::mem_pestat] mean and std.dev: (494.40, 459.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 772670 sequences (100000124 bp) in 3.236 CPU sec, 3.365 real sec... [M::process] read 772392 sequences (100000183 bp) in 2.500 CPU sec, 2.500 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 296107, 28, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 237, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1665) [M::mem_pestat] mean and std.dev: (380.63, 344.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (157.73, 64.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 1276, 2313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6439) [M::mem_pestat] mean and std.dev: (1281.28, 1323.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 308, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1379) [M::mem_pestat] mean and std.dev: (308.54, 223.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1784) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 773262 reads in 255.723 CPU sec, 8.352 real sec sorting block 56, #reads = 1088674 mem = 536876326 peak = 1026195456 [M::mem_process_seqs] Processed 772622 reads in 180.583 CPU sec, 5.760 real sec sorting block 57, #reads = 1086315 mem = 536880273 peak = 1040064512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 295474, 35, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 356, 1459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4061) [M::mem_pestat] mean and std.dev: (740.63, 940.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.01, 64.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (297, 884, 1319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3363) [M::mem_pestat] mean and std.dev: (799.13, 724.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4385) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 451, 802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1942) [M::mem_pestat] mean and std.dev: (538.46, 430.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2512) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 771832 sequences (100000138 bp) in 3.475 CPU sec, 3.680 real sec... [M::process] read 771534 sequences (100000005 bp) in 2.420 CPU sec, 2.428 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 295808, 35, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 243, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1542) [M::mem_pestat] mean and std.dev: (360.12, 317.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2009) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.38, 64.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (213, 653, 1667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4575) [M::mem_pestat] mean and std.dev: (920.48, 1019.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6029) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 386, 873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2233) [M::mem_pestat] mean and std.dev: (479.75, 392.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 772670 reads in 250.744 CPU sec, 8.241 real sec [M::mem_process_seqs] Processed 772392 reads in 176.801 CPU sec, 5.804 real sec sorting block 58, #reads = 1087195 mem = 536892763 peak = 1040064512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 295748, 45, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 365, 963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2537) [M::mem_pestat] mean and std.dev: (573.64, 605.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.30, 64.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (286, 865, 1685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4483) [M::mem_pestat] mean and std.dev: (1157.61, 1053.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5882) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 291, 1059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2909) [M::mem_pestat] mean and std.dev: (493.96, 567.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3834) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 770840 sequences (100000128 bp) in 3.511 CPU sec, 3.717 real sec... [M::process] read 771024 sequences (100000236 bp) in 2.460 CPU sec, 2.464 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 295768, 30, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 529, 1239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3327) [M::mem_pestat] mean and std.dev: (628.88, 603.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.64, 65.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (239, 656, 2221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6185) [M::mem_pestat] mean and std.dev: (1146.72, 1126.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8167) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 218, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (262.96, 172.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 771832 reads in 249.087 CPU sec, 8.078 real sec sorting block 59, #reads = 1086182 mem = 536881315 peak = 1040064512 [M::mem_process_seqs] Processed 771534 reads in 177.848 CPU sec, 5.931 real sec sorting block 60, #reads = 1085155 mem = 536878308 peak = 1040064512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 295111, 33, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 336, 915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2415) [M::mem_pestat] mean and std.dev: (585.36, 608.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3165) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (159.18, 65.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (207, 438, 1341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3609) [M::mem_pestat] mean and std.dev: (644.52, 570.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4743) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 300, 820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2130) [M::mem_pestat] mean and std.dev: (433.08, 357.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2785) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 770100 sequences (100000293 bp) in 3.302 CPU sec, 3.420 real sec... [M::process] read 769584 sequences (100000270 bp) in 2.560 CPU sec, 2.559 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 294662, 26, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 326, 757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1935) [M::mem_pestat] mean and std.dev: (440.54, 373.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (159.17, 65.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (294, 984, 1973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5331) [M::mem_pestat] mean and std.dev: (1255.80, 1245.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7010) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 261, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1665) [M::mem_pestat] mean and std.dev: (370.38, 339.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2162) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 770840 reads in 251.398 CPU sec, 8.239 real sec [M::mem_process_seqs] Processed 771024 reads in 175.545 CPU sec, 5.591 real sec sorting block 61, #reads = 1086883 mem = 536890781 peak = 1040064512 [M::process] read 769056 sequences (100000040 bp) in 2.528 CPU sec, 2.634 real sec... [M::process] read 768764 sequences (100000154 bp) in 2.301 CPU sec, 2.307 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 293542, 31, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 302, 693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1789) [M::mem_pestat] mean and std.dev: (410.67, 405.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.81, 65.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (335, 811, 1789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4697) [M::mem_pestat] mean and std.dev: (839.44, 697.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6151) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 344, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1949) [M::mem_pestat] mean and std.dev: (447.40, 428.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2535) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 769056 reads in 148.786 CPU sec, 4.739 real sec sorting block 62, #reads = 1084063 mem = 536880179 peak = 1040064512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 294685, 38, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 388, 1796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4986) [M::mem_pestat] mean and std.dev: (987.65, 1102.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.70, 65.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (244, 655, 1475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3937) [M::mem_pestat] mean and std.dev: (1041.35, 1061.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 289, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290) [M::mem_pestat] mean and std.dev: (350.58, 225.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 294083, 40, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 211, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1371) [M::mem_pestat] mean and std.dev: (357.90, 330.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.72, 65.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (257, 437, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2450) [M::mem_pestat] mean and std.dev: (490.49, 394.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3181) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (269, 568, 1322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3428) [M::mem_pestat] mean and std.dev: (661.09, 571.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4481) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 768662 sequences (100000235 bp) in 2.271 CPU sec, 2.273 real sec... [M::mem_process_seqs] Processed 770100 reads in 400.138 CPU sec, 13.018 real sec sorting block 63, #reads = 1082803 mem = 536881077 peak = 1040064512 [M::process] read 768046 sequences (100000013 bp) in 2.440 CPU sec, 2.445 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 293969, 31, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 320, 716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1750) [M::mem_pestat] mean and std.dev: (396.20, 310.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2267) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.90, 65.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (109, 442, 1200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3382) [M::mem_pestat] mean and std.dev: (610.57, 633.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4473) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 321, 1049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2767) [M::mem_pestat] mean and std.dev: (604.61, 661.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3626) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 768764 reads in 149.999 CPU sec, 5.013 real sec [M::mem_process_seqs] Processed 769584 reads in 500.759 CPU sec, 16.223 real sec sorting block 64, #reads = 1081776 mem = 536877658 peak = 1040064512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 293893, 45, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 430, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2095) [M::mem_pestat] mean and std.dev: (437.72, 372.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.96, 65.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (140, 394, 1108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3044) [M::mem_pestat] mean and std.dev: (562.45, 609.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4012) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 342, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2564) [M::mem_pestat] mean and std.dev: (430.51, 416.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 768226 sequences (100000174 bp) in 3.391 CPU sec, 3.548 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 293906, 39, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 265, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2728) [M::mem_pestat] mean and std.dev: (505.47, 610.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.44, 65.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (135, 283, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3540) [M::mem_pestat] mean and std.dev: (668.34, 833.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4675) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 374, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2243) [M::mem_pestat] mean and std.dev: (473.27, 405.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 767594 sequences (100000244 bp) in 2.370 CPU sec, 2.373 real sec... [M::mem_process_seqs] Processed 768662 reads in 289.482 CPU sec, 9.212 real sec sorting block 65, #reads = 1084586 mem = 536880738 peak = 1040064512 [M::mem_process_seqs] Processed 768046 reads in 182.110 CPU sec, 5.795 real sec sorting block 66, #reads = 1082695 mem = 536885321 peak = 1040064512 [M::process] read 767094 sequences (100000233 bp) in 3.287 CPU sec, 3.458 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 293771, 34, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 362, 858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2110) [M::mem_pestat] mean and std.dev: (528.66, 443.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.09, 66.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (218, 740, 1499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4061) [M::mem_pestat] mean and std.dev: (758.00, 686.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5342) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 289, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1691) [M::mem_pestat] mean and std.dev: (313.68, 243.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2200) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 767336 sequences (100000255 bp) in 2.360 CPU sec, 2.364 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 293805, 27, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 285, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2589) [M::mem_pestat] mean and std.dev: (485.12, 472.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3401) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (160.49, 65.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (224, 567, 1291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3425) [M::mem_pestat] mean and std.dev: (652.29, 577.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4492) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 373, 722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1756) [M::mem_pestat] mean and std.dev: (419.09, 338.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 767594 reads in 248.474 CPU sec, 7.884 real sec [M::mem_process_seqs] Processed 768226 reads in 295.286 CPU sec, 9.768 real sec sorting block 67, #reads = 1081786 mem = 536881961 peak = 1040064512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 293661, 37, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 266, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2002) [M::mem_pestat] mean and std.dev: (414.09, 352.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 149, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.32, 66.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 737, 963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2377) [M::mem_pestat] mean and std.dev: (612.34, 441.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3084) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 534, 1028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2620) [M::mem_pestat] mean and std.dev: (582.71, 449.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 767152 sequences (100000255 bp) in 3.711 CPU sec, 3.892 real sec... [M::process] read 767056 sequences (100000188 bp) in 2.467 CPU sec, 2.470 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 293619, 28, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 359, 1738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4864) [M::mem_pestat] mean and std.dev: (717.69, 909.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6427) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 149, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (161.71, 67.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (285, 572, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2655) [M::mem_pestat] mean and std.dev: (605.76, 586.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 295, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1977) [M::mem_pestat] mean and std.dev: (437.82, 357.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2571) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 767094 reads in 248.347 CPU sec, 7.875 real sec sorting block 68, #reads = 1082466 mem = 536877451 peak = 1040064512 [M::mem_process_seqs] Processed 767336 reads in 287.803 CPU sec, 9.556 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 293655, 40, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 358, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1772) [M::mem_pestat] mean and std.dev: (442.13, 390.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 149, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.34, 66.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (185, 514, 1845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5165) [M::mem_pestat] mean and std.dev: (801.86, 798.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6825) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 370, 1196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3200) [M::mem_pestat] mean and std.dev: (636.27, 576.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4202) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 767672 sequences (100000073 bp) in 3.363 CPU sec, 3.526 real sec... [M::process] read 777418 sequences (100000122 bp) in 2.870 CPU sec, 2.865 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 293586, 32, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 624, 1415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3749) [M::mem_pestat] mean and std.dev: (726.96, 616.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 149, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (161.66, 67.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (375, 724, 3259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9027) [M::mem_pestat] mean and std.dev: (1565.10, 1890.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11911) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 366, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3130) [M::mem_pestat] mean and std.dev: (645.00, 614.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4106) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 767152 reads in 251.642 CPU sec, 8.367 real sec sorting block 69, #reads = 1082467 mem = 536888787 peak = 1040064512 [M::mem_process_seqs] Processed 767056 reads in 293.414 CPU sec, 9.341 real sec sorting block 70, #reads = 1081240 mem = 536877598 peak = 1040064512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 294141, 32, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 223, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3125) [M::mem_pestat] mean and std.dev: (593.71, 726.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (161.15, 66.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (237, 876, 1508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4050) [M::mem_pestat] mean and std.dev: (925.13, 879.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 370, 944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2438) [M::mem_pestat] mean and std.dev: (472.10, 448.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3185) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 776792 sequences (100000063 bp) in 3.320 CPU sec, 3.484 real sec... [M::process] read 775978 sequences (100000029 bp) in 2.510 CPU sec, 2.504 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 297031, 39, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 250, 1297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3583) [M::mem_pestat] mean and std.dev: (579.97, 684.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (154.67, 61.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (327, 811, 3910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11076) [M::mem_pestat] mean and std.dev: (1896.46, 2421.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 304, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2603) [M::mem_pestat] mean and std.dev: (403.34, 357.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3417) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 767672 reads in 254.571 CPU sec, 8.087 real sec sorting block 71, #reads = 1081079 mem = 536882939 peak = 1040064512 [M::mem_process_seqs] Processed 777418 reads in 191.663 CPU sec, 6.112 real sec [M::process] read 775208 sequences (100000069 bp) in 3.020 CPU sec, 3.142 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 296986, 32, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 365, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2810) [M::mem_pestat] mean and std.dev: (506.07, 517.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 144, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (154.73, 61.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (306, 1461, 2597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7179) [M::mem_pestat] mean and std.dev: (1418.17, 1413.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 257, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1640) [M::mem_pestat] mean and std.dev: (342.18, 266.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2127) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 776792 reads in 151.878 CPU sec, 4.910 real sec sorting block 72, #reads = 1083142 mem = 536882164 peak = 1040064512 [M::process] read 774250 sequences (100000051 bp) in 2.751 CPU sec, 2.965 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 296716, 31, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 298, 924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2474) [M::mem_pestat] mean and std.dev: (419.88, 403.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (155.34, 62.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (667, 961, 1702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3772) [M::mem_pestat] mean and std.dev: (1125.67, 877.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4807) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 341, 772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1904) [M::mem_pestat] mean and std.dev: (400.67, 294.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2470) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 774320 sequences (100000132 bp) in 2.965 CPU sec, 2.971 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 296804, 30, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 242, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1751) [M::mem_pestat] mean and std.dev: (347.95, 333.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (155.72, 62.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (315, 1302, 2204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5982) [M::mem_pestat] mean and std.dev: (1329.77, 1074.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7871) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 239, 858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2248) [M::mem_pestat] mean and std.dev: (411.53, 490.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2943) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 296737, 24, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 253, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1418) [M::mem_pestat] mean and std.dev: (315.33, 263.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (156.44, 63.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (247, 704, 1428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3790) [M::mem_pestat] mean and std.dev: (693.10, 518.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4971) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 360, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2269) [M::mem_pestat] mean and std.dev: (465.77, 401.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2960) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 775978 reads in 388.132 CPU sec, 12.440 real sec sorting block 73, #reads = 1085785 mem = 536879430 peak = 1040064512 [M::mem_process_seqs] Processed 775208 reads in 288.765 CPU sec, 9.269 real sec [M::mem_process_seqs] Processed 774250 reads in 319.522 CPU sec, 10.254 real sec sorting block 74, #reads = 1085683 mem = 536878407 peak = 1040064512 [M::process] read 773712 sequences (100000284 bp) in 3.826 CPU sec, 4.125 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 296420, 37, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 257, 756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1974) [M::mem_pestat] mean and std.dev: (365.35, 316.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (156.65, 63.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 258, 885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2515) [M::mem_pestat] mean and std.dev: (390.45, 409.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 427, 1236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3222) [M::mem_pestat] mean and std.dev: (659.57, 659.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4215) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 772496 sequences (100000137 bp) in 3.213 CPU sec, 3.382 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 296605, 38, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 405, 1019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2653) [M::mem_pestat] mean and std.dev: (601.59, 577.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (156.72, 63.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (199, 476, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2770) [M::mem_pestat] mean and std.dev: (606.76, 588.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3627) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 271, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1662) [M::mem_pestat] mean and std.dev: (388.28, 318.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 772600 sequences (100000191 bp) in 2.240 CPU sec, 2.234 real sec... [M::mem_process_seqs] Processed 774320 reads in 279.198 CPU sec, 8.929 real sec sorting block 75, #reads = 1084788 mem = 536885015 peak = 1040064512 [M::mem_process_seqs] Processed 773712 reads in 193.975 CPU sec, 6.587 real sec sorting block 76, #reads = 1082476 mem = 536886367 peak = 1040064512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 296378, 37, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 293, 654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1578) [M::mem_pestat] mean and std.dev: (359.03, 243.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (157.45, 64.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (464, 955, 1889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4739) [M::mem_pestat] mean and std.dev: (913.58, 705.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6164) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 346, 1179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3149) [M::mem_pestat] mean and std.dev: (690.70, 716.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4134) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 771834 sequences (100000011 bp) in 4.390 CPU sec, 4.604 real sec... [M::process] read 772336 sequences (100000125 bp) in 2.724 CPU sec, 2.721 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 295908, 40, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 219, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1585) [M::mem_pestat] mean and std.dev: (398.81, 394.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (157.51, 63.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (244, 627, 1071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2725) [M::mem_pestat] mean and std.dev: (617.34, 497.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3552) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 377, 1403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3839) [M::mem_pestat] mean and std.dev: (804.09, 810.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5057) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 772600 reads in 254.445 CPU sec, 8.477 real sec [M::mem_process_seqs] Processed 772496 reads in 294.609 CPU sec, 9.803 real sec sorting block 77, #reads = 1085885 mem = 536880303 peak = 1040064512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 295642, 40, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 428, 1283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3463) [M::mem_pestat] mean and std.dev: (632.58, 657.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (157.76, 64.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (136, 507, 1290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3598) [M::mem_pestat] mean and std.dev: (725.00, 687.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4752) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 234, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1885) [M::mem_pestat] mean and std.dev: (413.55, 392.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2461) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 771576 sequences (100000265 bp) in 3.696 CPU sec, 3.943 real sec... [M::process] read 771368 sequences (100000135 bp) in 2.509 CPU sec, 2.506 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 295538, 33, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 285, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2528) [M::mem_pestat] mean and std.dev: (569.14, 553.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (157.69, 64.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 798, 1467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3897) [M::mem_pestat] mean and std.dev: (921.71, 824.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5112) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 415, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2876) [M::mem_pestat] mean and std.dev: (556.04, 464.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3771) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 771834 reads in 251.973 CPU sec, 8.028 real sec sorting block 78, #reads = 1084115 mem = 536880908 peak = 1040064512 [M::mem_process_seqs] Processed 772336 reads in 182.134 CPU sec, 5.911 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 294733, 33, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 235, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1560) [M::mem_pestat] mean and std.dev: (361.85, 279.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (157.99, 64.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 548, 2127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5981) [M::mem_pestat] mean and std.dev: (912.83, 1081.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 403, 998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2566) [M::mem_pestat] mean and std.dev: (516.52, 534.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 79, #reads = 1082909 mem = 536878727 peak = 1040064512 [M::process] read 770428 sequences (100000066 bp) in 3.518 CPU sec, 3.748 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 294513, 41, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 354, 1161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3141) [M::mem_pestat] mean and std.dev: (521.78, 564.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.35, 64.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (280, 827, 1947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5281) [M::mem_pestat] mean and std.dev: (1058.77, 905.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6948) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 425, 1101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2889) [M::mem_pestat] mean and std.dev: (656.55, 631.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3783) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 770318 sequences (100000247 bp) in 2.740 CPU sec, 2.737 real sec... [M::mem_process_seqs] Processed 771576 reads in 266.014 CPU sec, 8.572 real sec [M::mem_process_seqs] Processed 771368 reads in 190.241 CPU sec, 6.156 real sec sorting block 80, #reads = 1081889 mem = 536881479 peak = 1040064512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 294322, 28, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 235, 705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1805) [M::mem_pestat] mean and std.dev: (393.37, 394.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.83, 64.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 373, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2215) [M::mem_pestat] mean and std.dev: (427.19, 411.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2911) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 328, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3218) [M::mem_pestat] mean and std.dev: (616.15, 717.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4238) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 769890 sequences (100000097 bp) in 3.571 CPU sec, 3.765 real sec... [M::process] read 770380 sequences (100000170 bp) in 2.670 CPU sec, 2.675 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 293770, 33, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 252, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1402) [M::mem_pestat] mean and std.dev: (356.29, 308.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (159.16, 65.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (238, 547, 2195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6109) [M::mem_pestat] mean and std.dev: (930.52, 966.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8066) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 363, 804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2040) [M::mem_pestat] mean and std.dev: (428.07, 363.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 770428 reads in 257.934 CPU sec, 8.257 real sec sorting block 81, #reads = 1082361 mem = 536880337 peak = 1040064512 [M::mem_process_seqs] Processed 770318 reads in 183.386 CPU sec, 5.824 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 294075, 39, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 311, 725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1859) [M::mem_pestat] mean and std.dev: (409.85, 342.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (159.19, 64.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (117, 395, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1902) [M::mem_pestat] mean and std.dev: (411.00, 400.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 266, 732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1818) [M::mem_pestat] mean and std.dev: (420.46, 362.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2361) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 769514 sequences (100000050 bp) in 3.612 CPU sec, 3.776 real sec... [M::process] read 769166 sequences (100000034 bp) in 2.290 CPU sec, 2.282 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 294172, 29, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 428, 843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2209) [M::mem_pestat] mean and std.dev: (537.34, 509.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.93, 64.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (176, 512, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2543) [M::mem_pestat] mean and std.dev: (547.48, 486.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3332) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 289, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1472) [M::mem_pestat] mean and std.dev: (405.60, 318.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 769890 reads in 256.800 CPU sec, 8.174 real sec sorting block 82, #reads = 1080059 mem = 536884414 peak = 1040064512 [M::mem_process_seqs] Processed 770380 reads in 185.697 CPU sec, 6.492 real sec sorting block 83, #reads = 1080879 mem = 536878027 peak = 1063075840 [M::process] read 769194 sequences (100000030 bp) in 3.955 CPU sec, 4.109 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 294078, 37, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 359, 1021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2713) [M::mem_pestat] mean and std.dev: (615.96, 610.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.80, 65.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (265, 444, 1400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3670) [M::mem_pestat] mean and std.dev: (691.00, 577.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4805) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 681, 1104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2898) [M::mem_pestat] mean and std.dev: (766.04, 671.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 769146 sequences (100000093 bp) in 2.800 CPU sec, 2.802 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 293713, 33, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 201, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1672) [M::mem_pestat] mean and std.dev: (343.89, 334.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2184) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.60, 65.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (100, 504, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2725) [M::mem_pestat] mean and std.dev: (529.61, 514.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3600) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 605, 1224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3274) [M::mem_pestat] mean and std.dev: (739.45, 697.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 769166 reads in 271.668 CPU sec, 9.159 real sec [M::mem_process_seqs] Processed 769514 reads in 307.225 CPU sec, 10.577 real sec sorting block 84, #reads = 1080316 mem = 536881362 peak = 1063075840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 294043, 30, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 232, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1602) [M::mem_pestat] mean and std.dev: (327.85, 246.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2084) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.02, 65.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (242, 437, 1548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4160) [M::mem_pestat] mean and std.dev: (859.75, 1027.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5466) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 352, 1136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3062) [M::mem_pestat] mean and std.dev: (626.12, 724.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 769092 sequences (100000208 bp) in 3.527 CPU sec, 3.644 real sec... [M::mem_process_seqs] Processed 769194 reads in 157.745 CPU sec, 5.344 real sec sorting block 85, #reads = 1077746 mem = 536877388 peak = 1063075840 [M::process] read 769512 sequences (100000290 bp) in 3.609 CPU sec, 3.857 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 293800, 40, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 238, 1088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2876) [M::mem_pestat] mean and std.dev: (612.59, 689.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.81, 65.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (216, 473, 1662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4554) [M::mem_pestat] mean and std.dev: (859.11, 1007.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6000) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 492, 755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1829) [M::mem_pestat] mean and std.dev: (482.73, 384.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2366) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 770056 sequences (100000218 bp) in 2.203 CPU sec, 2.203 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 293683, 41, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 190, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (206.66, 115.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.04, 65.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (169, 739, 1466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4060) [M::mem_pestat] mean and std.dev: (821.95, 743.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5357) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 199, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1730) [M::mem_pestat] mean and std.dev: (369.37, 346.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2255) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 294262, 46, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 476, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2278) [M::mem_pestat] mean and std.dev: (533.09, 448.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (159.76, 65.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 359, 778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2220) [M::mem_pestat] mean and std.dev: (467.56, 499.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2941) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 274, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2108) [M::mem_pestat] mean and std.dev: (444.77, 397.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 769146 reads in 419.907 CPU sec, 13.487 real sec sorting block 86, #reads = 1078958 mem = 536878658 peak = 1063075840 [M::mem_process_seqs] Processed 769092 reads in 310.907 CPU sec, 9.999 real sec [M::mem_process_seqs] Processed 769512 reads in 343.826 CPU sec, 11.058 real sec sorting block 87, #reads = 1078773 mem = 536881592 peak = 1063075840 [M::process] read 778700 sequences (100000024 bp) in 4.779 CPU sec, 5.102 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 294310, 41, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 283, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2374) [M::mem_pestat] mean and std.dev: (511.32, 511.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3120) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.19, 64.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (148, 415, 1076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2932) [M::mem_pestat] mean and std.dev: (540.97, 491.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 373, 1124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3040) [M::mem_pestat] mean and std.dev: (510.45, 486.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3998) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 778764 sequences (100000225 bp) in 3.469 CPU sec, 3.605 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 297034, 49, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 262, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2499) [M::mem_pestat] mean and std.dev: (541.02, 597.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3284) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 142, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (153.54, 60.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (543, 943, 2193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5493) [M::mem_pestat] mean and std.dev: (941.63, 687.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7143) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 441, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3065) [M::mem_pestat] mean and std.dev: (650.98, 682.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 770056 reads in 278.403 CPU sec, 8.871 real sec [M::mem_process_seqs] Processed 778700 reads in 207.481 CPU sec, 6.548 real sec sorting block 88, #reads = 1078024 mem = 536878844 peak = 1063075840 [M::process] read 777040 sequences (100000058 bp) in 3.036 CPU sec, 3.144 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 297717, 26, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 254, 846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2210) [M::mem_pestat] mean and std.dev: (473.86, 485.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (153.70, 61.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (461, 1098, 3274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8900) [M::mem_pestat] mean and std.dev: (2080.12, 2408.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11715) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 490, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3246) [M::mem_pestat] mean and std.dev: (597.91, 623.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 89, #reads = 1077731 mem = 536883210 peak = 1063075840 [M::process] read 776122 sequences (100000083 bp) in 3.299 CPU sec, 3.572 real sec... [M::process] read 776058 sequences (100000039 bp) in 2.120 CPU sec, 2.121 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 296956, 35, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 289, 799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2019) [M::mem_pestat] mean and std.dev: (399.49, 348.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 144, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (154.70, 61.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (210, 396, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2424) [M::mem_pestat] mean and std.dev: (542.31, 526.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3162) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 363, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081) [M::mem_pestat] mean and std.dev: (444.04, 376.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2714) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 778764 reads in 269.643 CPU sec, 8.614 real sec [M::mem_process_seqs] Processed 777040 reads in 204.017 CPU sec, 6.530 real sec sorting block 90, #reads = 1080923 mem = 536883779 peak = 1063075840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 297567, 33, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 269, 1177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3209) [M::mem_pestat] mean and std.dev: (508.73, 577.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4225) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (155.34, 62.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (257, 436, 1016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2534) [M::mem_pestat] mean and std.dev: (494.71, 431.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3293) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 261, 1051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2775) [M::mem_pestat] mean and std.dev: (496.33, 597.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3637) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 775888 sequences (100000043 bp) in 3.404 CPU sec, 3.559 real sec... [M::process] read 775960 sequences (100000284 bp) in 2.550 CPU sec, 2.542 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 296968, 32, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 189, 946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2580) [M::mem_pestat] mean and std.dev: (509.90, 555.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (155.10, 62.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (459, 658, 1355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3147) [M::mem_pestat] mean and std.dev: (824.93, 673.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4043) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 271, 765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1907) [M::mem_pestat] mean and std.dev: (421.92, 353.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2478) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 776122 reads in 263.517 CPU sec, 8.376 real sec sorting block 91, #reads = 1083050 mem = 536883103 peak = 1063075840 [M::mem_process_seqs] Processed 776058 reads in 206.266 CPU sec, 6.596 real sec sorting block 92, #reads = 1083119 mem = 536882212 peak = 1063075840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 296957, 23, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 264, 693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1741) [M::mem_pestat] mean and std.dev: (342.16, 307.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2265) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (155.51, 62.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (307, 693, 1605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4201) [M::mem_pestat] mean and std.dev: (1018.55, 935.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5499) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 287, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1340) [M::mem_pestat] mean and std.dev: (344.36, 215.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1724) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 774328 sequences (100000166 bp) in 4.223 CPU sec, 4.472 real sec... [M::process] read 773142 sequences (100000049 bp) in 2.620 CPU sec, 2.619 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 297635, 24, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 281, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1737) [M::mem_pestat] mean and std.dev: (311.60, 313.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (154.95, 61.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (189, 763, 1588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4386) [M::mem_pestat] mean and std.dev: (775.36, 795.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5785) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 479, 977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2453) [M::mem_pestat] mean and std.dev: (600.14, 554.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 775888 reads in 275.656 CPU sec, 8.792 real sec [M::process] read 773336 sequences (100000037 bp) in 2.144 CPU sec, 2.155 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 296496, 35, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 266, 1167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3199) [M::mem_pestat] mean and std.dev: (672.22, 786.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4215) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (156.21, 62.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (242, 437, 1664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4508) [M::mem_pestat] mean and std.dev: (756.25, 845.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5930) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 220, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1809) [M::mem_pestat] mean and std.dev: (379.82, 355.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2357) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 774328 reads in 159.428 CPU sec, 5.490 real sec sorting block 93, #reads = 1081147 mem = 536877856 peak = 1063075840 [M::mem_process_seqs] Processed 775960 reads in 408.428 CPU sec, 13.503 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 296218, 38, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 265, 892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2362) [M::mem_pestat] mean and std.dev: (454.70, 495.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3097) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (156.88, 63.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 714, 2062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5704) [M::mem_pestat] mean and std.dev: (1365.79, 1384.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7525) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 196, 683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1749) [M::mem_pestat] mean and std.dev: (404.52, 429.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 94, #reads = 1081099 mem = 536889585 peak = 1063075840 [M::process] read 773000 sequences (100000161 bp) in 3.534 CPU sec, 4.028 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 296884, 30, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 319, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2037) [M::mem_pestat] mean and std.dev: (420.24, 351.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (157.11, 63.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (116, 453, 1312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3704) [M::mem_pestat] mean and std.dev: (568.26, 591.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4900) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 317, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1851) [M::mem_pestat] mean and std.dev: (371.92, 290.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2407) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 772088 sequences (100000063 bp) in 2.610 CPU sec, 2.607 real sec... [M::mem_process_seqs] Processed 773142 reads in 419.078 CPU sec, 13.318 real sec [M::mem_process_seqs] Processed 773336 reads in 192.579 CPU sec, 6.112 real sec sorting block 95, #reads = 1083087 mem = 536890433 peak = 1063075840 [M::process] read 772428 sequences (100000163 bp) in 3.314 CPU sec, 3.483 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 295437, 25, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 281, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2850) [M::mem_pestat] mean and std.dev: (616.89, 683.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (156.98, 63.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (202, 808, 3875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11221) [M::mem_pestat] mean and std.dev: (2274.72, 2962.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14894) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 320, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1774) [M::mem_pestat] mean and std.dev: (432.54, 335.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 773000 reads in 157.245 CPU sec, 5.291 real sec sorting block 96, #reads = 1083175 mem = 536881210 peak = 1063075840 [M::process] read 770950 sequences (100000162 bp) in 3.340 CPU sec, 3.513 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 295070, 34, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 281, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2366) [M::mem_pestat] mean and std.dev: (421.23, 390.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3102) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (157.55, 63.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (153, 505, 1776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5022) [M::mem_pestat] mean and std.dev: (901.03, 1106.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6645) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 326, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1839) [M::mem_pestat] mean and std.dev: (458.69, 390.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2391) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 771036 sequences (100000143 bp) in 2.754 CPU sec, 2.759 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 294738, 42, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 464, 1258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3496) [M::mem_pestat] mean and std.dev: (748.96, 930.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (157.38, 63.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (235, 642, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2131) [M::mem_pestat] mean and std.dev: (566.50, 456.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2763) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 321, 963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2573) [M::mem_pestat] mean and std.dev: (594.28, 597.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3378) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 772088 reads in 306.071 CPU sec, 9.782 real sec sorting block 97, #reads = 1080218 mem = 536880880 peak = 1063075840 [M::mem_process_seqs] Processed 772428 reads in 342.074 CPU sec, 11.141 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 294341, 28, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 301, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1738) [M::mem_pestat] mean and std.dev: (361.23, 317.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2267) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.29, 64.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (225, 801, 1918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5304) [M::mem_pestat] mean and std.dev: (1074.56, 1041.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6997) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 265, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1958) [M::mem_pestat] mean and std.dev: (471.02, 444.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 770456 sequences (100000178 bp) in 3.844 CPU sec, 4.104 real sec... [M::process] read 770158 sequences (100000162 bp) in 2.460 CPU sec, 2.457 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 293788, 39, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 288, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2297) [M::mem_pestat] mean and std.dev: (474.62, 488.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (158.03, 63.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (168, 282, 943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2493) [M::mem_pestat] mean and std.dev: (424.47, 369.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 300, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2625) [M::mem_pestat] mean and std.dev: (542.52, 587.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3441) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 770950 reads in 272.383 CPU sec, 8.840 real sec sorting block 98, #reads = 1081497 mem = 536877635 peak = 1063075840 [M::mem_process_seqs] Processed 771036 reads in 194.335 CPU sec, 6.206 real sec sorting block 99, #reads = 1079872 mem = 536884645 peak = 1063075840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 294182, 32, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 316, 720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1834) [M::mem_pestat] mean and std.dev: (433.36, 391.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2391) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.62, 64.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (163, 548, 1610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4504) [M::mem_pestat] mean and std.dev: (780.76, 825.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5951) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 317, 979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2581) [M::mem_pestat] mean and std.dev: (615.85, 638.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 769868 sequences (100000005 bp) in 3.711 CPU sec, 3.911 real sec... [M::process] read 770334 sequences (100000149 bp) in 2.617 CPU sec, 2.622 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 293486, 43, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 339, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1748) [M::mem_pestat] mean and std.dev: (418.58, 335.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (159.04, 64.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 486, 1069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2843) [M::mem_pestat] mean and std.dev: (661.05, 581.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3730) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1246) [M::mem_pestat] mean and std.dev: (307.40, 242.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 770456 reads in 263.163 CPU sec, 8.351 real sec sorting block 100, #reads = 1078831 mem = 536879592 peak = 1063075840 [M::mem_process_seqs] Processed 770158 reads in 190.073 CPU sec, 6.051 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 294229, 34, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 261, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1632) [M::mem_pestat] mean and std.dev: (380.23, 301.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.85, 64.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (198, 759, 2894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8286) [M::mem_pestat] mean and std.dev: (1609.82, 1868.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10982) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 568, 1592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4370) [M::mem_pestat] mean and std.dev: (890.58, 1050.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5759) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 769726 sequences (100000255 bp) in 3.937 CPU sec, 4.115 real sec... [M::process] read 769496 sequences (100000115 bp) in 2.300 CPU sec, 2.301 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 294085, 34, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 511, 1484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4116) [M::mem_pestat] mean and std.dev: (702.73, 731.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (158.87, 64.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (209, 398, 899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2279) [M::mem_pestat] mean and std.dev: (438.83, 351.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2969) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 254, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2771) [M::mem_pestat] mean and std.dev: (506.56, 543.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3638) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 770334 reads in 259.064 CPU sec, 8.225 real sec sorting block 101, #reads = 1080221 mem = 536891241 peak = 1063075840 [M::mem_process_seqs] Processed 769868 reads in 301.430 CPU sec, 10.174 real sec sorting block 102, #reads = 1080092 mem = 536889247 peak = 1063075840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 294029, 30, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 241, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1634) [M::mem_pestat] mean and std.dev: (398.84, 414.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2129) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (158.90, 64.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (447, 1285, 2161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5589) [M::mem_pestat] mean and std.dev: (1426.24, 1338.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7303) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 322, 916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2400) [M::mem_pestat] mean and std.dev: (483.22, 478.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3142) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 769690 sequences (100000281 bp) in 4.419 CPU sec, 4.664 real sec... [M::process] read 770408 sequences (100000025 bp) in 2.670 CPU sec, 2.669 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 293886, 36, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 454, 2310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6638) [M::mem_pestat] mean and std.dev: (1067.03, 1216.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.94, 64.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (150, 782, 1727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4881) [M::mem_pestat] mean and std.dev: (955.89, 1024.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6458) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 372, 1548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4278) [M::mem_pestat] mean and std.dev: (647.09, 816.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5643) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 769496 reads in 280.905 CPU sec, 9.480 real sec [M::mem_process_seqs] Processed 769726 reads in 315.817 CPU sec, 10.822 real sec sorting block 103, #reads = 1079071 mem = 536880095 peak = 1063075840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 293911, 41, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 209, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559) [M::mem_pestat] mean and std.dev: (311.95, 273.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.99, 64.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (100, 416, 1084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3052) [M::mem_pestat] mean and std.dev: (559.68, 575.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4036) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 306, 981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2625) [M::mem_pestat] mean and std.dev: (430.25, 416.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3447) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 777628 sequences (100000040 bp) in 4.132 CPU sec, 4.399 real sec... [M::process] read 776698 sequences (100000120 bp) in 2.769 CPU sec, 2.771 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 293695, 38, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 319, 1628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4546) [M::mem_pestat] mean and std.dev: (867.42, 1103.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.45, 64.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (339, 685, 1719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4479) [M::mem_pestat] mean and std.dev: (1049.86, 1009.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5859) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 329, 691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1739) [M::mem_pestat] mean and std.dev: (360.28, 299.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2263) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 769690 reads in 298.645 CPU sec, 9.695 real sec sorting block 104, #reads = 1079409 mem = 536881247 peak = 1063075840 [M::mem_process_seqs] Processed 770408 reads in 217.677 CPU sec, 6.949 real sec sorting block 105, #reads = 1072836 mem = 536887131 peak = 1063075840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 296634, 25, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 206, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1532) [M::mem_pestat] mean and std.dev: (368.66, 343.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1993) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (154.18, 61.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (166, 485, 1064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2860) [M::mem_pestat] mean and std.dev: (516.59, 452.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3758) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 233, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1289) [M::mem_pestat] mean and std.dev: (282.07, 212.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 774802 sequences (100000281 bp) in 3.923 CPU sec, 4.153 real sec... [M::process] read 774338 sequences (100000055 bp) in 2.560 CPU sec, 2.559 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 297395, 28, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 398, 1161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3129) [M::mem_pestat] mean and std.dev: (561.29, 554.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4113) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 144, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (155.32, 62.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (398, 740, 3806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10622) [M::mem_pestat] mean and std.dev: (1666.75, 2110.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14030) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 295, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1612) [M::mem_pestat] mean and std.dev: (423.53, 361.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2088) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 777628 reads in 289.695 CPU sec, 9.478 real sec [M::mem_process_seqs] Processed 776698 reads in 194.040 CPU sec, 6.187 real sec sorting block 106, #reads = 1072825 mem = 536881393 peak = 1063075840 [M::process] read 774108 sequences (100000050 bp) in 3.078 CPU sec, 3.203 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 297068, 31, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 274, 791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1971) [M::mem_pestat] mean and std.dev: (495.83, 497.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (156.26, 63.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (134, 287, 916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2480) [M::mem_pestat] mean and std.dev: (504.97, 564.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3262) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 319, 907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2295) [M::mem_pestat] mean and std.dev: (499.49, 433.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2989) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 774802 reads in 148.607 CPU sec, 5.052 real sec sorting block 107, #reads = 1084935 mem = 536878077 peak = 1063075840 [M::process] read 774124 sequences (100000082 bp) in 3.696 CPU sec, 4.082 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 296562, 40, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 219, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (262.98, 188.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (156.50, 63.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (77, 233, 1066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3044) [M::mem_pestat] mean and std.dev: (500.59, 608.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4033) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 318, 799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2039) [M::mem_pestat] mean and std.dev: (498.15, 487.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 773270 sequences (100000282 bp) in 2.617 CPU sec, 2.620 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 296675, 42, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 353, 1221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3299) [M::mem_pestat] mean and std.dev: (755.77, 773.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4338) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (156.74, 63.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (118, 471, 1296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3652) [M::mem_pestat] mean and std.dev: (679.78, 899.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4830) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 282, 1034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2784) [M::mem_pestat] mean and std.dev: (613.11, 699.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 774338 reads in 296.693 CPU sec, 9.476 real sec [M::mem_process_seqs] Processed 774108 reads in 329.864 CPU sec, 10.781 real sec sorting block 108, #reads = 1085958 mem = 536883514 peak = 1063075840 [M::process] read 773148 sequences (100000025 bp) in 3.966 CPU sec, 4.230 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 296185, 38, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 463, 1571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4397) [M::mem_pestat] mean and std.dev: (819.95, 894.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (157.22, 63.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (286, 683, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2767) [M::mem_pestat] mean and std.dev: (573.84, 387.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3594) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 354, 782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2014) [M::mem_pestat] mean and std.dev: (456.24, 445.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2630) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 773212 sequences (100000278 bp) in 2.540 CPU sec, 2.539 real sec... [M::mem_process_seqs] Processed 773270 reads in 147.390 CPU sec, 4.672 real sec sorting block 109, #reads = 1084498 mem = 536892247 peak = 1063075840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 296296, 35, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 253, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2181) [M::mem_pestat] mean and std.dev: (437.80, 472.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (156.57, 63.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (125, 510, 1390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3920) [M::mem_pestat] mean and std.dev: (629.87, 701.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5185) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 402, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2178) [M::mem_pestat] mean and std.dev: (545.25, 518.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 296815, 33, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 206, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2197) [M::mem_pestat] mean and std.dev: (421.88, 476.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (157.10, 63.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (179, 660, 1104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2954) [M::mem_pestat] mean and std.dev: (609.43, 552.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3879) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 237, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1917) [M::mem_pestat] mean and std.dev: (400.07, 337.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2499) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 773162 sequences (100000067 bp) in 2.788 CPU sec, 2.792 real sec... [M::mem_process_seqs] Processed 774124 reads in 397.903 CPU sec, 12.887 real sec sorting block 110, #reads = 1084547 mem = 536881264 peak = 1063075840 [M::mem_process_seqs] Processed 773148 reads in 228.181 CPU sec, 7.296 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 296320, 47, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 262, 1054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2872) [M::mem_pestat] mean and std.dev: (567.68, 614.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (157.15, 63.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 404, 804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1944) [M::mem_pestat] mean and std.dev: (485.30, 365.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2514) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 409, 962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2438) [M::mem_pestat] mean and std.dev: (591.57, 561.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 772562 sequences (100000022 bp) in 3.680 CPU sec, 3.881 real sec... [M::process] read 772494 sequences (100000170 bp) in 1.990 CPU sec, 1.992 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 295851, 30, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 288, 630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1530) [M::mem_pestat] mean and std.dev: (353.40, 275.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (157.47, 64.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (230, 816, 1728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4724) [M::mem_pestat] mean and std.dev: (1046.66, 1123.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6222) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 263, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1386) [M::mem_pestat] mean and std.dev: (321.49, 234.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 773212 reads in 270.497 CPU sec, 8.609 real sec sorting block 111, #reads = 1086401 mem = 536881019 peak = 1063075840 [M::process] read 772006 sequences (100000301 bp) in 2.955 CPU sec, 2.963 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 296001, 33, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 233, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1894) [M::mem_pestat] mean and std.dev: (395.59, 393.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (157.66, 64.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (355, 590, 3092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8566) [M::mem_pestat] mean and std.dev: (1624.82, 2085.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11303) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 292, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1402) [M::mem_pestat] mean and std.dev: (372.28, 313.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1812) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 772494 reads in 146.773 CPU sec, 4.664 real sec sorting block 112, #reads = 1084535 mem = 536880520 peak = 1063075840 [M::mem_process_seqs] Processed 773162 reads in 387.053 CPU sec, 12.341 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 296416, 33, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 390, 1201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3289) [M::mem_pestat] mean and std.dev: (643.93, 738.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4333) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (157.61, 63.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (178, 526, 1471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4057) [M::mem_pestat] mean and std.dev: (913.12, 972.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 381, 1358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3670) [M::mem_pestat] mean and std.dev: (607.30, 639.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 113, #reads = 1078408 mem = 536878988 peak = 1063075840 [M::process] read 770834 sequences (100000014 bp) in 3.706 CPU sec, 3.953 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 296029, 31, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 283, 1154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3152) [M::mem_pestat] mean and std.dev: (526.70, 633.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.09, 64.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (167, 407, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2348) [M::mem_pestat] mean and std.dev: (528.86, 481.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3075) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 301, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2235) [M::mem_pestat] mean and std.dev: (434.79, 426.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 770958 sequences (100000263 bp) in 1.880 CPU sec, 1.872 real sec... [M::mem_process_seqs] Processed 772562 reads in 389.586 CPU sec, 12.362 real sec [M::mem_process_seqs] Processed 772006 reads in 188.683 CPU sec, 6.326 real sec sorting block 114, #reads = 1084237 mem = 536877841 peak = 1063075840 [M::process] read 770296 sequences (100000088 bp) in 3.597 CPU sec, 3.847 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 294351, 29, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 326, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1508) [M::mem_pestat] mean and std.dev: (375.68, 276.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1954) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.86, 64.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (274, 702, 1571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4165) [M::mem_pestat] mean and std.dev: (766.92, 771.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5462) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 397, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2264) [M::mem_pestat] mean and std.dev: (489.53, 416.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 769802 sequences (100000080 bp) in 1.960 CPU sec, 1.968 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 293827, 31, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 257, 886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2322) [M::mem_pestat] mean and std.dev: (496.58, 499.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.34, 64.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (155, 426, 1038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2804) [M::mem_pestat] mean and std.dev: (539.14, 588.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3687) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 257, 781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2037) [M::mem_pestat] mean and std.dev: (443.75, 436.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 770834 reads in 260.842 CPU sec, 8.626 real sec sorting block 115, #reads = 1081086 mem = 536882565 peak = 1063075840 [M::mem_process_seqs] Processed 770958 reads in 294.820 CPU sec, 9.834 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 294141, 37, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 451, 1203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3301) [M::mem_pestat] mean and std.dev: (703.30, 722.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4350) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.90, 64.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (248, 567, 1492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3980) [M::mem_pestat] mean and std.dev: (903.97, 878.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 437, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3002) [M::mem_pestat] mean and std.dev: (688.28, 698.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 770120 sequences (100000171 bp) in 3.383 CPU sec, 3.580 real sec... [M::process] read 769698 sequences (100000149 bp) in 2.020 CPU sec, 2.020 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 293384, 50, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 294, 1110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2964) [M::mem_pestat] mean and std.dev: (584.70, 640.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (159.05, 64.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (222, 650, 1393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3735) [M::mem_pestat] mean and std.dev: (849.15, 835.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4906) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 275, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1727) [M::mem_pestat] mean and std.dev: (417.30, 400.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 770296 reads in 255.039 CPU sec, 8.211 real sec sorting block 116, #reads = 1078507 mem = 536877628 peak = 1063075840 [M::mem_process_seqs] Processed 769802 reads in 189.814 CPU sec, 6.070 real sec sorting block 117, #reads = 1079267 mem = 536882467 peak = 1063075840 [M::process] read 769518 sequences (100000232 bp) in 2.973 CPU sec, 3.117 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 293578, 48, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 259, 914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2438) [M::mem_pestat] mean and std.dev: (511.70, 569.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.93, 64.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (218, 707, 1458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3938) [M::mem_pestat] mean and std.dev: (932.68, 879.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5178) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 393, 1093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2867) [M::mem_pestat] mean and std.dev: (595.64, 584.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3754) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 770120 reads in 150.286 CPU sec, 4.811 real sec sorting block 118, #reads = 1077907 mem = 536881845 peak = 1063075840 [M::process] read 769578 sequences (100000194 bp) in 3.190 CPU sec, 3.305 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 293993, 37, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 228, 732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1902) [M::mem_pestat] mean and std.dev: (317.60, 268.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.10, 64.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (185, 673, 1356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3698) [M::mem_pestat] mean and std.dev: (840.03, 851.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4869) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 361, 1149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3029) [M::mem_pestat] mean and std.dev: (644.23, 716.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3969) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 770170 sequences (100000126 bp) in 2.916 CPU sec, 2.922 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 293815, 38, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 276, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2132) [M::mem_pestat] mean and std.dev: (485.06, 460.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (158.97, 64.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (345, 673, 1875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4935) [M::mem_pestat] mean and std.dev: (1078.83, 1044.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6465) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 301, 1423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3927) [M::mem_pestat] mean and std.dev: (570.19, 636.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5179) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 769698 reads in 324.567 CPU sec, 10.352 real sec [M::mem_process_seqs] Processed 769518 reads in 356.007 CPU sec, 11.477 real sec sorting block 119, #reads = 1070604 mem = 536879517 peak = 1063075840 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 293545, 25, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 380, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2648) [M::mem_pestat] mean and std.dev: (559.84, 521.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (159.05, 64.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (223, 760, 3021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8617) [M::mem_pestat] mean and std.dev: (1633.24, 2030.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 291, 831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2157) [M::mem_pestat] mean and std.dev: (501.28, 500.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 770218 sequences (100000119 bp) in 3.322 CPU sec, 3.483 real sec... [M::process] read 775668 sequences (100000009 bp) in 2.820 CPU sec, 2.826 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 294559, 33, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 308, 1228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3398) [M::mem_pestat] mean and std.dev: (594.39, 782.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.87, 64.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (146, 732, 1693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4787) [M::mem_pestat] mean and std.dev: (876.33, 839.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6334) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 506, 1345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3613) [M::mem_pestat] mean and std.dev: (696.17, 657.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4747) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 769578 reads in 268.113 CPU sec, 8.605 real sec sorting block 120, #reads = 1076445 mem = 536880717 peak = 1063075840 [M::mem_process_seqs] Processed 770170 reads in 192.726 CPU sec, 6.138 real sec [M::process] read 780832 sequences (100000192 bp) in 2.573 CPU sec, 2.681 real sec... sorting block 121, #reads = 1076012 mem = 536879974 peak = 1063075840 [M::process] read 778824 sequences (100000001 bp) in 2.569 CPU sec, 2.574 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 298597, 34, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 283, 672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1738) [M::mem_pestat] mean and std.dev: (397.96, 368.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2271) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (107, 142, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (152.61, 60.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 698, 968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2614) [M::mem_pestat] mean and std.dev: (613.33, 529.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 271, 952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2540) [M::mem_pestat] mean and std.dev: (517.21, 593.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3334) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 780832 reads in 156.486 CPU sec, 4.996 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 293402, 35, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 246, 918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2462) [M::mem_pestat] mean and std.dev: (463.29, 448.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3234) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.46, 64.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (303, 558, 2430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6684) [M::mem_pestat] mean and std.dev: (1568.31, 1832.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8897) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 530, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2939) [M::mem_pestat] mean and std.dev: (749.18, 701.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3844) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 294518, 35, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 279, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1341) [M::mem_pestat] mean and std.dev: (345.34, 273.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (154.99, 61.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (266, 1008, 2388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6632) [M::mem_pestat] mean and std.dev: (1326.64, 1415.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8754) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 365, 1808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5050) [M::mem_pestat] mean and std.dev: (813.35, 1015.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6671) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 777564 sequences (100000029 bp) in 2.759 CPU sec, 2.759 real sec... [M::mem_process_seqs] Processed 770218 reads in 457.022 CPU sec, 14.855 real sec sorting block 122, #reads = 1079563 mem = 536879045 peak = 1063075840 [M::process] read 776788 sequences (100000129 bp) in 2.972 CPU sec, 2.986 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 297834, 40, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 356, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3000) [M::mem_pestat] mean and std.dev: (501.25, 471.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (154.21, 61.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 651, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3124) [M::mem_pestat] mean and std.dev: (643.14, 490.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4093) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 323, 986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2610) [M::mem_pestat] mean and std.dev: (569.04, 602.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3422) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 777564 reads in 160.943 CPU sec, 5.186 real sec sorting block 123, #reads = 1077057 mem = 536880834 peak = 1063075840 [M::mem_process_seqs] Processed 775668 reads in 588.559 CPU sec, 18.856 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 298049, 30, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 237, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (288.27, 203.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (153.67, 60.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (230, 790, 2359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6617) [M::mem_pestat] mean and std.dev: (1363.10, 1488.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8746) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 316, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1919) [M::mem_pestat] mean and std.dev: (433.48, 380.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2494) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 777734 sequences (100000009 bp) in 3.273 CPU sec, 3.492 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 297464, 32, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 402, 1306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3582) [M::mem_pestat] mean and std.dev: (754.98, 922.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 144, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (154.86, 61.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (186, 411, 1265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3423) [M::mem_pestat] mean and std.dev: (757.23, 778.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 401, 1195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3259) [M::mem_pestat] mean and std.dev: (705.40, 745.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4291) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 778516 sequences (100000001 bp) in 2.440 CPU sec, 2.433 real sec... [M::mem_process_seqs] Processed 778824 reads in 431.227 CPU sec, 13.784 real sec sorting block 124, #reads = 1066832 mem = 536881155 peak = 1063075840 [M::mem_process_seqs] Processed 776788 reads in 197.356 CPU sec, 6.615 real sec sorting block 125, #reads = 1081148 mem = 536884485 peak = 1069760512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 297697, 33, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 196, 722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1872) [M::mem_pestat] mean and std.dev: (384.93, 375.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (154.16, 61.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (238, 543, 997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2515) [M::mem_pestat] mean and std.dev: (561.97, 543.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 351, 886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2272) [M::mem_pestat] mean and std.dev: (525.74, 473.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 776324 sequences (100000047 bp) in 4.181 CPU sec, 4.427 real sec... [M::process] read 776012 sequences (100000035 bp) in 2.670 CPU sec, 2.666 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 297767, 25, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 240, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1914) [M::mem_pestat] mean and std.dev: (456.02, 435.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (108, 143, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (153.94, 61.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (222, 430, 779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1893) [M::mem_pestat] mean and std.dev: (535.83, 457.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2450) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 226, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1606) [M::mem_pestat] mean and std.dev: (351.83, 322.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2091) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 777734 reads in 286.322 CPU sec, 9.398 real sec sorting block 126, #reads = 1081937 mem = 536878543 peak = 1069760512 [M::mem_process_seqs] Processed 778516 reads in 336.950 CPU sec, 11.059 real sec [M::process] read 774680 sequences (100000184 bp) in 3.100 CPU sec, 3.200 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 297478, 38, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 242, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1253) [M::mem_pestat] mean and std.dev: (283.45, 252.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (154.87, 61.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (244, 554, 1588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4276) [M::mem_pestat] mean and std.dev: (993.03, 1013.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5620) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 342, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1848) [M::mem_pestat] mean and std.dev: (424.70, 322.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2401) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 776324 reads in 155.065 CPU sec, 4.993 real sec sorting block 127, #reads = 1078573 mem = 536879579 peak = 1069760512 [M::process] read 774770 sequences (100000197 bp) in 2.431 CPU sec, 2.556 real sec... [M::process] read 774136 sequences (100000183 bp) in 3.005 CPU sec, 3.014 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 296755, 23, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 329, 800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2098) [M::mem_pestat] mean and std.dev: (471.81, 495.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (155.57, 62.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (202, 880, 1778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4930) [M::mem_pestat] mean and std.dev: (1016.26, 985.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6506) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 351, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2433) [M::mem_pestat] mean and std.dev: (482.81, 425.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3185) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 774770 reads in 155.639 CPU sec, 4.969 real sec sorting block 128, #reads = 1080915 mem = 536884656 peak = 1069760512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 297693, 26, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 269, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1669) [M::mem_pestat] mean and std.dev: (377.82, 328.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 144, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (154.85, 61.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (107, 509, 1972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5702) [M::mem_pestat] mean and std.dev: (947.79, 1133.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7567) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 254, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (274.88, 172.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1329) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 296128, 43, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 437, 1375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3733) [M::mem_pestat] mean and std.dev: (640.98, 671.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (155.81, 62.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (104, 378, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2552) [M::mem_pestat] mean and std.dev: (552.34, 551.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 358, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2034) [M::mem_pestat] mean and std.dev: (473.22, 361.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2635) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 772738 sequences (100000184 bp) in 2.969 CPU sec, 2.975 real sec... [M::mem_process_seqs] Processed 776012 reads in 460.185 CPU sec, 14.678 real sec sorting block 129, #reads = 1082684 mem = 536883001 peak = 1069760512 [M::mem_process_seqs] Processed 774680 reads in 340.363 CPU sec, 11.063 real sec [M::process] read 771978 sequences (100000154 bp) in 3.694 CPU sec, 3.827 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 296095, 34, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 265, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2815) [M::mem_pestat] mean and std.dev: (459.67, 447.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3704) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (109, 145, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (156.24, 62.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (196, 689, 1728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4792) [M::mem_pestat] mean and std.dev: (960.72, 1045.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6324) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 621, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3057) [M::mem_pestat] mean and std.dev: (682.31, 673.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 770798 sequences (100000234 bp) in 2.560 CPU sec, 2.560 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 295216, 50, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 295, 698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1772) [M::mem_pestat] mean and std.dev: (365.57, 285.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 145, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (157.01, 63.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 679, 1679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4651) [M::mem_pestat] mean and std.dev: (836.65, 805.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6137) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 294, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1526) [M::mem_pestat] mean and std.dev: (359.32, 275.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1969) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 774136 reads in 264.407 CPU sec, 8.595 real sec sorting block 130, #reads = 1079203 mem = 536878477 peak = 1069760512 [M::mem_process_seqs] Processed 772738 reads in 300.922 CPU sec, 10.089 real sec sorting block 131, #reads = 1080316 mem = 536879732 peak = 1069760512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 294157, 49, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 311, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2684) [M::mem_pestat] mean and std.dev: (416.40, 432.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.24, 64.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (199, 565, 1300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3502) [M::mem_pestat] mean and std.dev: (641.69, 616.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4603) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 345, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1904) [M::mem_pestat] mean and std.dev: (434.12, 409.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2481) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 770588 sequences (100000159 bp) in 3.978 CPU sec, 4.169 real sec... [M::process] read 770138 sequences (100000099 bp) in 2.540 CPU sec, 2.536 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 294476, 51, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 243, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278) [M::mem_pestat] mean and std.dev: (325.95, 268.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (157.57, 63.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (448, 842, 2588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6868) [M::mem_pestat] mean and std.dev: (1562.86, 1523.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9008) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 478, 890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2290) [M::mem_pestat] mean and std.dev: (526.35, 531.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2990) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 770798 reads in 264.504 CPU sec, 8.688 real sec [M::mem_process_seqs] Processed 771978 reads in 300.817 CPU sec, 10.149 real sec sorting block 132, #reads = 1079305 mem = 536885046 peak = 1069760512 [M::process] read 770154 sequences (100000268 bp) in 3.161 CPU sec, 3.296 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 293695, 38, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 274, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2109) [M::mem_pestat] mean and std.dev: (390.60, 378.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (158.82, 64.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (190, 560, 1343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3649) [M::mem_pestat] mean and std.dev: (763.31, 745.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 351, 978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2548) [M::mem_pestat] mean and std.dev: (497.62, 486.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 769126 sequences (100000141 bp) in 1.955 CPU sec, 1.962 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 293578, 38, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 532, 1604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4448) [M::mem_pestat] mean and std.dev: (872.22, 1016.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.66, 64.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (222, 880, 1461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3939) [M::mem_pestat] mean and std.dev: (894.91, 846.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5178) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 306, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2741) [M::mem_pestat] mean and std.dev: (513.25, 531.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3596) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 294369, 35, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 323, 1000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2698) [M::mem_pestat] mean and std.dev: (457.84, 464.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.60, 64.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (424, 928, 2400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6352) [M::mem_pestat] mean and std.dev: (1350.70, 1327.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8328) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 320, 1130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3040) [M::mem_pestat] mean and std.dev: (563.96, 590.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 770588 reads in 368.359 CPU sec, 12.039 real sec sorting block 133, #reads = 1077257 mem = 536876375 peak = 1069760512 [M::mem_process_seqs] Processed 770154 reads in 293.452 CPU sec, 9.444 real sec [M::mem_process_seqs] Processed 770138 reads in 334.432 CPU sec, 10.763 real sec sorting block 134, #reads = 1078564 mem = 536882362 peak = 1069760512 [M::process] read 769000 sequences (100000148 bp) in 4.285 CPU sec, 4.531 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 293258, 33, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 264, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2013) [M::mem_pestat] mean and std.dev: (445.40, 443.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.70, 65.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (226, 475, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2620) [M::mem_pestat] mean and std.dev: (633.93, 602.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3418) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 345, 1046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2730) [M::mem_pestat] mean and std.dev: (569.26, 544.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3572) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 768272 sequences (100000152 bp) in 3.577 CPU sec, 3.810 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 293335, 36, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 441, 1274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3492) [M::mem_pestat] mean and std.dev: (645.32, 654.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.82, 65.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (151, 419, 907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2419) [M::mem_pestat] mean and std.dev: (552.79, 566.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3175) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 244, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2579) [M::mem_pestat] mean and std.dev: (491.81, 497.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3386) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 769126 reads in 261.253 CPU sec, 8.341 real sec sorting block 135, #reads = 1079338 mem = 536882540 peak = 1069760512 [M::mem_process_seqs] Processed 769000 reads in 191.461 CPU sec, 6.044 real sec [M::process] read 768638 sequences (100000067 bp) in 3.498 CPU sec, 3.705 real sec... sorting block 136, #reads = 1079573 mem = 536885576 peak = 1069760512 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 293112, 36, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 291, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3302) [M::mem_pestat] mean and std.dev: (654.40, 715.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (160.05, 65.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 653, 1649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4803) [M::mem_pestat] mean and std.dev: (976.82, 1253.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 358, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2358) [M::mem_pestat] mean and std.dev: (536.24, 524.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3076) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 769128 sequences (100000014 bp) in 3.268 CPU sec, 3.378 real sec... [M::process] read 509740 sequences (66178068 bp) in 1.740 CPU sec, 1.745 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 293249, 34, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 426, 1052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2782) [M::mem_pestat] mean and std.dev: (555.54, 514.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 148, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.80, 65.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 455, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1606) [M::mem_pestat] mean and std.dev: (400.73, 414.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2099) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 268, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1663) [M::mem_pestat] mean and std.dev: (383.95, 301.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2161) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 768272 reads in 264.202 CPU sec, 8.433 real sec sorting block 137, #reads = 1078006 mem = 536882667 peak = 1069760512 [M::mem_process_seqs] Processed 768638 reads in 193.284 CPU sec, 6.193 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 194341, 26, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 696, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2614) [M::mem_pestat] mean and std.dev: (558.04, 443.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (110, 147, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (158.65, 64.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (176, 684, 2807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8069) [M::mem_pestat] mean and std.dev: (1520.23, 1957.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10700) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 413, 798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1900) [M::mem_pestat] mean and std.dev: (469.49, 329.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2451) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 294000, 34, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 266, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3095) [M::mem_pestat] mean and std.dev: (561.02, 606.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (111, 147, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (159.44, 65.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (157, 639, 1585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4441) [M::mem_pestat] mean and std.dev: (734.84, 862.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5869) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 283, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1855) [M::mem_pestat] mean and std.dev: (419.38, 369.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2410) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 509740 reads in 219.115 CPU sec, 7.080 real sec sorting block 138, #reads = 1077878 mem = 536884198 peak = 1069760512 [M::mem_process_seqs] Processed 769128 reads in 262.249 CPU sec, 8.483 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: INTEL(R) XEON(R) PLATINUM 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 149373468 overall: 72262189056 mem 29174.329 user 508.169 sys 977.377 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_81F-T1-TDNA-1_A23MHWWLT4_1.L002\tPU:L002\tSM:1136_81F-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_81F-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_81F-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_81F-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 977.414 sec; CPU: 29682.502 sec sorting block 139, #reads = 1045168 mem = 521499337 peak = 1069760512 uniq header count 1 merging 140 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: INTEL(R) XEON(R) PLATINUM 8559C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 8871083966 output reads: 151460203 bam_mem_sort: 140 calls 72.121 user 1.588 sys 73.973 real bam_write: 140 calls 40.431 user 2.481 sys 43.114 real execute: 1 calls 54.872 user 1.945 sys 1043.487 real merge_files: 1 calls 54.843 user 1.920 sys 65.163 real parse_chunk: 2240 calls 123.886 user 30.431 sys 156.450 real read_chunk: 6118 calls 72.280 user 47.661 sys 946.536 real sort_block: 1 calls 46.684 user 47.035 sys 913.606 real write_chunk: 3876 calls 54.338 user 1.550 sys 63.872 real overall: 4603588608 mem 2524.629 user 104.822 sys 1043.508 real ls: cannot access '*.cram': No such file or directory