cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1234.951 CPU sec, 340.045 real sec
[M::process] read 704386 sequences (100000094 bp) in 0.989 CPU sec, 0.996 real sec...
[M::process] read 701938 sequences (100000138 bp) in 1.192 CPU sec, 1.233 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293163, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (234.75, 111.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 257, 1079)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2777)
[M::mem_pestat] mean and std.dev: (556.77, 429.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3626)
[M::mem_pestat] skip orientation RR
[M::process] read 701330 sequences (100000228 bp) in 1.159 CPU sec, 1.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291738, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.79, 115.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700592 sequences (100000084 bp) in 1.160 CPU sec, 1.163 real sec...
[M::mem_process_seqs] Processed 704386 reads in 121.224 CPU sec, 3.843 real sec
[M::mem_process_seqs] Processed 701938 reads in 77.182 CPU sec, 2.450 real sec
sorting block 0, #reads = 1095070 mem = 536877537 peak = 609116160
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292178, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.74, 116.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 506, 663)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1569)
[M::mem_pestat] mean and std.dev: (480.92, 232.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2022)
[M::mem_pestat] skip orientation RR
[M::process] read 699690 sequences (100000211 bp) in 1.709 CPU sec, 1.751 real sec...
[M::process] read 699106 sequences (100000167 bp) in 1.230 CPU sec, 1.232 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291816, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (246.03, 118.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701330 reads in 117.008 CPU sec, 3.709 real sec
[M::mem_process_seqs] Processed 700592 reads in 76.623 CPU sec, 2.435 real sec
sorting block 1, #reads = 1094286 mem = 536882952 peak = 738091008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291647, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.77, 120.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698934 sequences (100000254 bp) in 1.686 CPU sec, 1.732 real sec...
[M::process] read 698334 sequences (100000178 bp) in 1.240 CPU sec, 1.235 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291375, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.68, 119.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699690 reads in 115.372 CPU sec, 3.658 real sec
[M::mem_process_seqs] Processed 699106 reads in 75.125 CPU sec, 2.387 real sec
sorting block 2, #reads = 1094758 mem = 536880635 peak = 879398912
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291118, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.17, 121.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698416 sequences (100000263 bp) in 2.292 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290936, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.60, 122.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698934 reads in 117.183 CPU sec, 3.715 real sec
sorting block 3, #reads = 1094122 mem = 536878119 peak = 879398912
[M::mem_process_seqs] Processed 698334 reads in 77.662 CPU sec, 2.453 real sec
[M::process] read 697640 sequences (100000056 bp) in 1.462 CPU sec, 1.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291144, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.61, 122.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1093072 mem = 536880368 peak = 992509952
[M::process] read 696826 sequences (100000136 bp) in 1.595 CPU sec, 1.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290907, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 224, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (248.30, 105.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 696858 sequences (100000292 bp) in 1.240 CPU sec, 1.241 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.37, 121.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698416 reads in 117.702 CPU sec, 3.762 real sec
[M::mem_process_seqs] Processed 697640 reads in 77.149 CPU sec, 2.461 real sec
sorting block 5, #reads = 1093434 mem = 536881311 peak = 992509952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 290265, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.80, 119.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697002 sequences (100000136 bp) in 2.342 CPU sec, 2.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290367, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 222, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863)
[M::mem_pestat] mean and std.dev: (244.11, 92.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1093)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.37, 118.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696826 reads in 121.095 CPU sec, 3.846 real sec
[M::mem_process_seqs] Processed 696858 reads in 79.666 CPU sec, 2.520 real sec
[M::process] read 696886 sequences (100000170 bp) in 1.985 CPU sec, 2.072 real sec...
sorting block 6, #reads = 1091113 mem = 536883524 peak = 992509952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290214, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (250.09, 117.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697102 sequences (100000200 bp) in 2.087 CPU sec, 2.229 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290585, 5, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 335, 611)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1461)
[M::mem_pestat] mean and std.dev: (380.45, 346.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1886)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (250.97, 117.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 224, 405)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835)
[M::mem_pestat] mean and std.dev: (264.92, 131.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1050)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697002 reads in 121.262 CPU sec, 3.874 real sec
[M::mem_process_seqs] Processed 696886 reads in 79.247 CPU sec, 2.509 real sec
sorting block 7, #reads = 1091788 mem = 536878859 peak = 992509952
[M::process] read 697116 sequences (100000180 bp) in 2.328 CPU sec, 2.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290487, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.05, 118.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (109, 245, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (182.73, 93.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290709, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 442, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132)
[M::mem_pestat] mean and std.dev: (381.92, 228.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1437)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.58, 118.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696138 sequences (100000062 bp) in 2.367 CPU sec, 2.511 real sec...
[M::mem_process_seqs] Processed 697102 reads in 123.389 CPU sec, 3.963 real sec
sorting block 8, #reads = 1090849 mem = 536879780 peak = 992509952
[M::mem_process_seqs] Processed 697116 reads in 80.839 CPU sec, 2.577 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290305, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.04, 120.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 236, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (271.60, 107.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RR
[M::process] read 699746 sequences (100000094 bp) in 2.448 CPU sec, 2.644 real sec...
[M::mem_process_seqs] Processed 696138 reads in 69.895 CPU sec, 2.305 real sec
sorting block 9, #reads = 1092530 mem = 536878199 peak = 992509952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290950, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (245.70, 116.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699746 reads in 71.070 CPU sec, 2.332 real sec
[M::process] read 702500 sequences (100000073 bp) in 2.444 CPU sec, 2.623 real sec...
sorting block 10, #reads = 1089851 mem = 536878972 peak = 992509952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292223, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.20, 114.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 371, 742)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1774)
[M::mem_pestat] mean and std.dev: (473.00, 321.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2290)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702500 reads in 67.961 CPU sec, 2.231 real sec
[M::process] read 701740 sequences (100000301 bp) in 2.668 CPU sec, 2.899 real sec...
sorting block 11, #reads = 1093723 mem = 536879398 peak = 992509952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291854, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.31, 116.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701740 reads in 67.458 CPU sec, 2.190 real sec
[M::process] read 701394 sequences (100000126 bp) in 2.312 CPU sec, 2.442 real sec...
[M::process] read 700952 sequences (100000021 bp) in 1.666 CPU sec, 1.706 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291660, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.39, 118.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 172, 193)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (115, 245)
[M::mem_pestat] mean and std.dev: (168.00, 26.86)
[M::mem_pestat] low and high boundaries for proper pairs: (61, 275)
[M::mem_pestat] skip orientation RR
[M::process] read 699992 sequences (100000155 bp) in 1.240 CPU sec, 1.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291397, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 287, 867)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2269)
[M::mem_pestat] mean and std.dev: (403.67, 340.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2970)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (246.90, 119.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701394 reads in 119.733 CPU sec, 3.770 real sec
sorting block 12, #reads = 1096109 mem = 536878115 peak = 992509952
[M::mem_process_seqs] Processed 700952 reads in 78.591 CPU sec, 2.473 real sec
[M::process] read 699134 sequences (100000200 bp) in 1.427 CPU sec, 1.453 real sec...
sorting block 13, #reads = 1095474 mem = 536879869 peak = 992509952
[M::process] read 699000 sequences (100000252 bp) in 2.593 CPU sec, 2.791 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291351, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (250.90, 121.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699134 reads in 69.778 CPU sec, 2.207 real sec
[M::process] read 698384 sequences (100000036 bp) in 1.903 CPU sec, 2.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291592, 10, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 195, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1163)
[M::mem_pestat] mean and std.dev: (306.67, 282.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1497)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (249.40, 120.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (8, 143, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (91.67, 103.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 772)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 184, 259)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (10, 425)
[M::mem_pestat] mean and std.dev: (198.09, 55.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 508)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291576, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (244, 262, 1539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4129)
[M::mem_pestat] mean and std.dev: (824.62, 859.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5424)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (251.67, 121.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699992 reads in 192.198 CPU sec, 6.120 real sec
[M::mem_process_seqs] Processed 699000 reads in 93.899 CPU sec, 2.983 real sec
sorting block 14, #reads = 1094492 mem = 536877511 peak = 992509952
[M::process] read 697946 sequences (100000162 bp) in 2.031 CPU sec, 2.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 291010, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 184, 270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562)
[M::mem_pestat] mean and std.dev: (174.67, 67.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 708)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (253.05, 122.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 15, #reads = 1095789 mem = 536878609 peak = 992509952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290560, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (253.98, 122.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697250 sequences (100000234 bp) in 2.396 CPU sec, 2.597 real sec...
[M::mem_process_seqs] Processed 698384 reads in 123.523 CPU sec, 3.958 real sec
[M::mem_process_seqs] Processed 697946 reads in 78.893 CPU sec, 2.508 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290987, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.45, 120.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696944 sequences (100000125 bp) in 2.261 CPU sec, 2.493 real sec...
[M::mem_process_seqs] Processed 697250 reads in 66.315 CPU sec, 2.205 real sec
sorting block 16, #reads = 1094288 mem = 536880583 peak = 992509952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290825, 10, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.84, 119.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (231, 673, 1111)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2871)
[M::mem_pestat] mean and std.dev: (515.00, 380.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3751)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 230, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537)
[M::mem_pestat] mean and std.dev: (185.50, 59.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 665)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 697212 sequences (100000024 bp) in 2.166 CPU sec, 2.346 real sec...
[M::mem_process_seqs] Processed 696944 reads in 67.887 CPU sec, 2.245 real sec
sorting block 17, #reads = 1093330 mem = 536879669 peak = 992509952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 289893, 6, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 157, 1186)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3290)
[M::mem_pestat] mean and std.dev: (605.73, 771.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4342)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.45, 118.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 294, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269)
[M::mem_pestat] mean and std.dev: (317.10, 213.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1635)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 697052 sequences (100000200 bp) in 2.152 CPU sec, 2.333 real sec...
[M::mem_process_seqs] Processed 697212 reads in 68.380 CPU sec, 2.260 real sec
sorting block 18, #reads = 1092282 mem = 536884509 peak = 992509952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290344, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.29, 118.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697046 sequences (100000095 bp) in 2.052 CPU sec, 2.183 real sec...
[M::mem_process_seqs] Processed 697052 reads in 68.674 CPU sec, 2.259 real sec
[M::process] read 697556 sequences (100000159 bp) in 1.820 CPU sec, 1.902 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289948, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.60, 118.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697122 sequences (100000147 bp) in 1.290 CPU sec, 1.287 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290416, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.93, 118.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697046 reads in 121.202 CPU sec, 3.819 real sec
sorting block 19, #reads = 1091022 mem = 536879101 peak = 993058816
[M::mem_process_seqs] Processed 697556 reads in 78.539 CPU sec, 2.474 real sec
[M::process] read 697108 sequences (100000183 bp) in 1.452 CPU sec, 1.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290855, 7, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 280, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (37, 497)
[M::mem_pestat] mean and std.dev: (249.12, 43.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 589)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.73, 120.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 20, #reads = 1092366 mem = 536879724 peak = 1022640128
[M::process] read 703696 sequences (100000073 bp) in 1.806 CPU sec, 1.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290396, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 211, 1152)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3166)
[M::mem_pestat] mean and std.dev: (600.67, 596.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4173)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.38, 120.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697122 reads in 124.403 CPU sec, 3.970 real sec
[M::process] read 703274 sequences (100000138 bp) in 1.348 CPU sec, 1.358 real sec...
[M::mem_process_seqs] Processed 697108 reads in 82.153 CPU sec, 2.605 real sec
sorting block 21, #reads = 1091260 mem = 536877773 peak = 1022640128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292277, 9, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (237.90, 113.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 263, 1398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3772)
[M::mem_pestat] mean and std.dev: (832.23, 853.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4959)
[M::mem_pestat] skip orientation RR
[M::process] read 702970 sequences (100000025 bp) in 1.754 CPU sec, 1.864 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292554, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.29, 115.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702146 sequences (100000232 bp) in 1.310 CPU sec, 1.307 real sec...
[M::mem_process_seqs] Processed 703696 reads in 120.712 CPU sec, 3.846 real sec
[M::mem_process_seqs] Processed 703274 reads in 78.449 CPU sec, 2.490 real sec
sorting block 22, #reads = 1092154 mem = 536879621 peak = 1022640128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292415, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (131, 207, 810)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2168)
[M::mem_pestat] mean and std.dev: (420.70, 537.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2847)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (239.88, 115.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701204 sequences (100000141 bp) in 1.973 CPU sec, 2.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292648, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.26, 116.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702970 reads in 119.783 CPU sec, 3.813 real sec
[M::mem_process_seqs] Processed 702146 reads in 78.252 CPU sec, 2.473 real sec
[M::process] read 700944 sequences (100000301 bp) in 1.821 CPU sec, 1.871 real sec...
sorting block 23, #reads = 1096446 mem = 536877793 peak = 1022640128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 292270, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 397, 1503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4101)
[M::mem_pestat] mean and std.dev: (582.38, 602.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5400)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.63, 119.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 24, #reads = 1095320 mem = 536878810 peak = 1043038208
[M::process] read 699800 sequences (100000162 bp) in 2.084 CPU sec, 2.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292386, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.84, 118.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701204 reads in 120.530 CPU sec, 3.855 real sec
[M::mem_process_seqs] Processed 700944 reads in 78.722 CPU sec, 2.494 real sec
[M::process] read 699360 sequences (100000148 bp) in 1.921 CPU sec, 2.032 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291226, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (248.94, 120.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1096176 mem = 536881560 peak = 1043038208
[M::process] read 699428 sequences (100000180 bp) in 1.903 CPU sec, 1.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291379, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (250.40, 121.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 246, 622)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1504)
[M::mem_pestat] mean and std.dev: (439.36, 374.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1945)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699800 reads in 120.662 CPU sec, 3.861 real sec
[M::mem_process_seqs] Processed 699360 reads in 79.391 CPU sec, 2.513 real sec
[M::process] read 698824 sequences (100000236 bp) in 1.660 CPU sec, 1.705 real sec...
sorting block 26, #reads = 1095966 mem = 536878402 peak = 1043038208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291319, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.73, 121.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 256, 256)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444)
[M::mem_pestat] mean and std.dev: (243.70, 69.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 538)
[M::mem_pestat] skip orientation RR
[M::process] read 698878 sequences (100000126 bp) in 1.828 CPU sec, 1.901 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291243, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.72, 121.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 417, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (320.45, 144.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699428 reads in 120.031 CPU sec, 3.819 real sec
[M::process] read 698598 sequences (100000174 bp) in 1.375 CPU sec, 1.381 real sec...
[M::mem_process_seqs] Processed 698824 reads in 78.630 CPU sec, 2.495 real sec
sorting block 27, #reads = 1094132 mem = 536879096 peak = 1044799488
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290859, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (246.57, 116.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697690 sequences (100000224 bp) in 2.466 CPU sec, 2.657 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290896, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.82, 119.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698878 reads in 122.435 CPU sec, 3.901 real sec
sorting block 28, #reads = 1093038 mem = 536879298 peak = 1044799488
[M::mem_process_seqs] Processed 698598 reads in 80.482 CPU sec, 2.551 real sec
[M::process] read 697406 sequences (100000142 bp) in 1.632 CPU sec, 1.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290990, 7, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.80, 121.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 298, 811)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1933)
[M::mem_pestat] mean and std.dev: (388.82, 274.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2494)
[M::mem_pestat] skip orientation RR
sorting block 29, #reads = 1093102 mem = 536880122 peak = 1087942656
[M::process] read 697430 sequences (100000291 bp) in 1.893 CPU sec, 1.999 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291081, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.83, 121.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697690 reads in 123.375 CPU sec, 3.929 real sec
[M::process] read 697780 sequences (100000046 bp) in 1.459 CPU sec, 1.472 real sec...
[M::mem_process_seqs] Processed 697406 reads in 80.281 CPU sec, 2.548 real sec
sorting block 30, #reads = 1092532 mem = 536882033 peak = 1087942656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290511, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.15, 120.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696792 sequences (100000111 bp) in 1.891 CPU sec, 1.972 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290728, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (251.92, 120.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702048 sequences (100000208 bp) in 1.330 CPU sec, 1.332 real sec...
[M::mem_process_seqs] Processed 697430 reads in 122.840 CPU sec, 3.910 real sec
[M::mem_process_seqs] Processed 697780 reads in 79.042 CPU sec, 2.510 real sec
sorting block 31, #reads = 1091840 mem = 536879196 peak = 1087942656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290257, 7, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.80, 122.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 909, 2490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7166)
[M::mem_pestat] mean and std.dev: (1456.27, 1609.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9504)
[M::mem_pestat] skip orientation RR
[M::process] read 703276 sequences (100000097 bp) in 2.298 CPU sec, 2.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291588, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.77, 115.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696792 reads in 121.308 CPU sec, 3.877 real sec
[M::mem_process_seqs] Processed 702048 reads in 80.209 CPU sec, 2.543 real sec
sorting block 32, #reads = 1091265 mem = 536879444 peak = 1087942656
[M::process] read 702484 sequences (100000034 bp) in 1.817 CPU sec, 1.906 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292510, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (238.94, 114.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1091722 mem = 536878607 peak = 1087942656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292194, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.34, 114.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (482, 638, 1387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3197)
[M::mem_pestat] mean and std.dev: (648.90, 420.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4102)
[M::mem_pestat] skip orientation RR
[M::process] read 702722 sequences (100000143 bp) in 2.088 CPU sec, 2.269 real sec...
[M::mem_process_seqs] Processed 703276 reads in 117.148 CPU sec, 3.758 real sec
[M::mem_process_seqs] Processed 702484 reads in 77.651 CPU sec, 2.464 real sec
[M::process] read 701690 sequences (100000157 bp) in 1.738 CPU sec, 1.812 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292434, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.99, 115.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 34, #reads = 1097304 mem = 536879436 peak = 1087942656
[M::process] read 701502 sequences (100000055 bp) in 1.739 CPU sec, 1.802 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292381, 7, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 829, 869)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2243)
[M::mem_pestat] mean and std.dev: (500.22, 353.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2930)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.11, 117.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 252, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522)
[M::mem_pestat] mean and std.dev: (221.75, 101.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 643)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702722 reads in 117.961 CPU sec, 3.757 real sec
[M::mem_process_seqs] Processed 701690 reads in 77.641 CPU sec, 2.455 real sec
[M::process] read 700896 sequences (100000092 bp) in 1.799 CPU sec, 1.914 real sec...
sorting block 35, #reads = 1097093 mem = 536877298 peak = 1087942656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292198, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (244.86, 118.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291625, 11, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 194, 912)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2464)
[M::mem_pestat] mean and std.dev: (386.70, 346.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3240)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.67, 119.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (457, 457, 553)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (265, 745)
[M::mem_pestat] mean and std.dev: (494.33, 38.83)
[M::mem_pestat] low and high boundaries for proper pairs: (169, 841)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 209, 485)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137)
[M::mem_pestat] mean and std.dev: (249.70, 122.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1463)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700042 sequences (100000083 bp) in 2.423 CPU sec, 2.660 real sec...
[M::mem_process_seqs] Processed 701502 reads in 118.266 CPU sec, 3.799 real sec
sorting block 36, #reads = 1095895 mem = 536881386 peak = 1087942656
[M::process] read 700104 sequences (100000161 bp) in 2.000 CPU sec, 2.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291826, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.64, 119.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 280, 597)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1475)
[M::mem_pestat] mean and std.dev: (396.00, 260.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1914)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700042 reads in 66.853 CPU sec, 2.123 real sec
[M::mem_process_seqs] Processed 700896 reads in 186.126 CPU sec, 5.905 real sec
[M::process] read 699600 sequences (100000215 bp) in 1.717 CPU sec, 1.786 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291974, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (249.46, 121.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 37, #reads = 1094723 mem = 536879509 peak = 1087942656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291166, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (250.16, 121.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (323, 854, 952)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2210)
[M::mem_pestat] mean and std.dev: (669.55, 328.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2839)
[M::mem_pestat] skip orientation RR
[M::process] read 698904 sequences (100000057 bp) in 2.013 CPU sec, 2.157 real sec...
[M::mem_process_seqs] Processed 700104 reads in 131.713 CPU sec, 4.174 real sec
[M::mem_process_seqs] Processed 699600 reads in 78.998 CPU sec, 2.511 real sec
sorting block 38, #reads = 1095267 mem = 536879071 peak = 1097949184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290996, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (251.22, 121.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (313, 392, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (155, 550)
[M::mem_pestat] mean and std.dev: (343.00, 58.72)
[M::mem_pestat] low and high boundaries for proper pairs: (76, 629)
[M::mem_pestat] skip orientation RR
[M::process] read 698220 sequences (100000008 bp) in 2.236 CPU sec, 2.443 real sec...
[M::mem_process_seqs] Processed 698904 reads in 66.839 CPU sec, 2.229 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291174, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.54, 121.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697148 sequences (100000204 bp) in 2.366 CPU sec, 2.624 real sec...
sorting block 39, #reads = 1094915 mem = 536878317 peak = 1097949184
[M::mem_process_seqs] Processed 698220 reads in 67.126 CPU sec, 2.230 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291214, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (255.17, 122.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 460, 1187)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3529)
[M::mem_pestat] mean and std.dev: (616.67, 915.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4700)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 696610 sequences (100000174 bp) in 1.900 CPU sec, 1.985 real sec...
[M::mem_process_seqs] Processed 697148 reads in 67.828 CPU sec, 2.232 real sec
sorting block 40, #reads = 1093528 mem = 536877897 peak = 1130729472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290551, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (256.38, 122.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696584 sequences (100000192 bp) in 2.034 CPU sec, 2.123 real sec...
[M::mem_process_seqs] Processed 696610 reads in 67.557 CPU sec, 2.213 real sec
sorting block 41, #reads = 1091762 mem = 536877277 peak = 1141166080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290216, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.29, 121.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696398 sequences (100000033 bp) in 2.256 CPU sec, 2.469 real sec...
[M::mem_process_seqs] Processed 696584 reads in 67.930 CPU sec, 2.202 real sec
sorting block 42, #reads = 1091888 mem = 536878002 peak = 1141166080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290224, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.37, 120.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696332 sequences (100000279 bp) in 1.848 CPU sec, 1.910 real sec...
[M::mem_process_seqs] Processed 696398 reads in 67.906 CPU sec, 2.191 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289953, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.93, 120.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700624 sequences (100000163 bp) in 2.189 CPU sec, 2.412 real sec...
[M::mem_process_seqs] Processed 696332 reads in 68.836 CPU sec, 2.239 real sec
sorting block 43, #reads = 1089075 mem = 536877589 peak = 1141166080
[M::process] read 707030 sequences (100000037 bp) in 1.890 CPU sec, 1.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291419, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (244.22, 116.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 706048 sequences (100000120 bp) in 1.340 CPU sec, 1.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293746, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (229.05, 109.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700624 reads in 129.426 CPU sec, 4.070 real sec
[M::mem_process_seqs] Processed 707030 reads in 83.674 CPU sec, 2.634 real sec
[M::process] read 704262 sequences (100000126 bp) in 1.527 CPU sec, 1.591 real sec...
sorting block 44, #reads = 1088615 mem = 536878916 peak = 1141166080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293585, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (230.35, 109.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702844 sequences (100000207 bp) in 2.449 CPU sec, 2.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293144, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (235.63, 113.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 706048 reads in 126.583 CPU sec, 4.045 real sec
[M::mem_process_seqs] Processed 704262 reads in 82.299 CPU sec, 2.608 real sec
sorting block 45, #reads = 1096100 mem = 536882524 peak = 1141166080
[M::process] read 701880 sequences (100000159 bp) in 1.998 CPU sec, 2.148 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292791, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 304, 1092)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2888)
[M::mem_pestat] mean and std.dev: (474.00, 393.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3786)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (240.12, 116.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701604 sequences (100000243 bp) in 1.838 CPU sec, 1.954 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292322, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.95, 118.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702844 reads in 122.746 CPU sec, 3.925 real sec
sorting block 46, #reads = 1094823 mem = 536877814 peak = 1179258880
[M::mem_process_seqs] Processed 701880 reads in 80.339 CPU sec, 2.546 real sec
[M::process] read 700808 sequences (100000119 bp) in 1.657 CPU sec, 1.707 real sec...
sorting block 47, #reads = 1095232 mem = 536876953 peak = 1181413376
[M::process] read 701194 sequences (100000189 bp) in 1.915 CPU sec, 1.992 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291863, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (247.29, 121.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700808 reads in 68.946 CPU sec, 2.170 real sec
[M::process] read 699404 sequences (100000257 bp) in 1.314 CPU sec, 1.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292131, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (244.78, 119.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291909, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.29, 121.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 221, 918)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2364)
[M::mem_pestat] mean and std.dev: (386.90, 357.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3087)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701604 reads in 190.976 CPU sec, 6.067 real sec
sorting block 48, #reads = 1094185 mem = 536880184 peak = 1181413376
[M::process] read 698534 sequences (100000107 bp) in 1.777 CPU sec, 1.801 real sec...
[M::mem_process_seqs] Processed 701194 reads in 93.711 CPU sec, 2.976 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291524, 2, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.86, 120.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 231, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (220.67, 121.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RR
sorting block 49, #reads = 1092806 mem = 536879003 peak = 1184780288
[M::process] read 698056 sequences (100000059 bp) in 2.283 CPU sec, 2.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290909, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.31, 121.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 424, 739)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1831)
[M::mem_pestat] mean and std.dev: (436.08, 334.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2377)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699404 reads in 125.882 CPU sec, 4.013 real sec
[M::mem_process_seqs] Processed 698534 reads in 83.303 CPU sec, 2.633 real sec
[M::process] read 698460 sequences (100000050 bp) in 1.809 CPU sec, 1.927 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 291082, 7, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 692, 822)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2174)
[M::mem_pestat] mean and std.dev: (679.56, 624.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3176)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.93, 120.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 258, 3189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9209)
[M::mem_pestat] mean and std.dev: (816.50, 1189.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12219)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 50, #reads = 1091425 mem = 536881790 peak = 1184780288
[M::process] read 699196 sequences (100000239 bp) in 2.300 CPU sec, 2.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290500, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.74, 119.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 254, 650)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1584)
[M::mem_pestat] mean and std.dev: (379.10, 253.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2051)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698056 reads in 125.759 CPU sec, 4.017 real sec
[M::mem_process_seqs] Processed 698460 reads in 83.262 CPU sec, 2.643 real sec
[M::process] read 698566 sequences (100000159 bp) in 2.128 CPU sec, 2.284 real sec...
sorting block 51, #reads = 1090898 mem = 536878139 peak = 1184780288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 291163, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 190, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (66, 326)
[M::mem_pestat] mean and std.dev: (178.46, 36.99)
[M::mem_pestat] low and high boundaries for proper pairs: (14, 378)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.68, 119.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698042 sequences (100000046 bp) in 1.778 CPU sec, 1.849 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290927, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 213, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (213.33, 105.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 902)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.70, 119.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699196 reads in 127.565 CPU sec, 4.087 real sec
[M::mem_process_seqs] Processed 698566 reads in 83.952 CPU sec, 2.654 real sec
sorting block 52, #reads = 1090354 mem = 536878500 peak = 1184780288
[M::process] read 698114 sequences (100000240 bp) in 1.766 CPU sec, 1.825 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291036, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097)
[M::mem_pestat] mean and std.dev: (273.12, 134.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1411)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.20, 120.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 53, #reads = 1090348 mem = 536877938 peak = 1190699008
[M::process] read 707432 sequences (100000147 bp) in 1.950 CPU sec, 2.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290602, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.59, 120.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698042 reads in 129.174 CPU sec, 4.102 real sec
[M::process] read 707642 sequences (100000255 bp) in 1.378 CPU sec, 1.382 real sec...
[M::mem_process_seqs] Processed 698114 reads in 84.755 CPU sec, 2.684 real sec
sorting block 54, #reads = 1089111 mem = 536878565 peak = 1190699008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 294016, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (228.51, 108.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 707104 sequences (100000001 bp) in 1.934 CPU sec, 2.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 294040, 1, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 347, 1040)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2748)
[M::mem_pestat] mean and std.dev: (448.00, 382.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3602)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 705298 sequences (100000104 bp) in 1.450 CPU sec, 1.446 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (146, 204, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587)
[M::mem_pestat] mean and std.dev: (227.25, 107.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 734)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 707432 reads in 139.559 CPU sec, 4.439 real sec
[M::mem_process_seqs] Processed 707642 reads in 89.419 CPU sec, 2.841 real sec
sorting block 55, #reads = 1090555 mem = 536876714 peak = 1190699008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293625, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 206, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (229.13, 108.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704368 sequences (100000217 bp) in 2.032 CPU sec, 2.093 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293093, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (233.48, 111.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703080 sequences (100000105 bp) in 1.460 CPU sec, 1.455 real sec...
[M::mem_process_seqs] Processed 707104 reads in 138.047 CPU sec, 4.391 real sec
[M::mem_process_seqs] Processed 705298 reads in 89.677 CPU sec, 2.849 real sec
sorting block 56, #reads = 1090810 mem = 536882082 peak = 1190699008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293455, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (235.52, 113.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 355, 790)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2018)
[M::mem_pestat] mean and std.dev: (364.25, 257.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2632)
[M::mem_pestat] skip orientation RR
[M::process] read 702112 sequences (100000109 bp) in 1.997 CPU sec, 2.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292739, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.73, 114.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704368 reads in 131.726 CPU sec, 4.201 real sec
[M::process] read 701848 sequences (100000084 bp) in 1.610 CPU sec, 1.646 real sec...
sorting block 57, #reads = 1089639 mem = 536879895 peak = 1195696128
[M::mem_process_seqs] Processed 703080 reads in 84.696 CPU sec, 2.674 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292731, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.62, 116.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1091844 mem = 536879188 peak = 1196359680
[M::process] read 701370 sequences (100000038 bp) in 2.062 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292426, 10, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (242.01, 117.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (692, 692, 852)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (372, 1172)
[M::mem_pestat] mean and std.dev: (708.56, 126.94)
[M::mem_pestat] low and high boundaries for proper pairs: (201, 1332)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 702112 reads in 126.872 CPU sec, 4.040 real sec
[M::process] read 700000 sequences (100000156 bp) in 1.484 CPU sec, 1.494 real sec...
[M::mem_process_seqs] Processed 701848 reads in 82.493 CPU sec, 2.611 real sec
sorting block 59, #reads = 1092275 mem = 536877681 peak = 1196359680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291894, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.71, 119.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699288 sequences (100000021 bp) in 1.894 CPU sec, 1.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291778, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (246.49, 118.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701370 reads in 124.891 CPU sec, 3.975 real sec
[M::process] read 698678 sequences (100000198 bp) in 1.448 CPU sec, 1.449 real sec...
[M::mem_process_seqs] Processed 700000 reads in 82.415 CPU sec, 2.611 real sec
sorting block 60, #reads = 1092033 mem = 536877828 peak = 1196359680
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291482, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.36, 120.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 412, 722)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1872)
[M::mem_pestat] mean and std.dev: (440.17, 294.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2447)
[M::mem_pestat] skip orientation RR
[M::process] read 698958 sequences (100000231 bp) in 1.981 CPU sec, 2.061 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291554, 2, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (249.06, 120.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 220, 643)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1629)
[M::mem_pestat] mean and std.dev: (314.77, 222.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2122)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699288 reads in 124.061 CPU sec, 3.944 real sec
[M::process] read 698810 sequences (100000110 bp) in 1.738 CPU sec, 1.763 real sec...
[M::mem_process_seqs] Processed 698678 reads in 80.957 CPU sec, 2.553 real sec
sorting block 61, #reads = 1091816 mem = 536879228 peak = 1197772800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290668, 9, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 221, 1581)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4345)
[M::mem_pestat] mean and std.dev: (346.70, 416.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5727)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.16, 119.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 62, #reads = 1091480 mem = 536879872 peak = 1197772800
[M::process] read 698924 sequences (100000035 bp) in 2.363 CPU sec, 2.578 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291025, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.67, 120.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698958 reads in 125.434 CPU sec, 4.023 real sec
[M::mem_process_seqs] Processed 698810 reads in 83.430 CPU sec, 2.643 real sec
[M::process] read 698324 sequences (100000193 bp) in 2.343 CPU sec, 2.551 real sec...
sorting block 63, #reads = 1090112 mem = 536877370 peak = 1197772800
[M::process] read 706536 sequences (100000154 bp) in 1.836 CPU sec, 1.903 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290813, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (249.15, 120.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698324 reads in 78.799 CPU sec, 2.485 real sec
[M::process] read 706578 sequences (100000296 bp) in 1.686 CPU sec, 1.728 real sec...
sorting block 64, #reads = 1086491 mem = 536880250 peak = 1197772800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290880, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (248.15, 120.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 195, 229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 359)
[M::mem_pestat] mean and std.dev: (183.60, 54.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 424)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293662, 8, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 263, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (255.22, 94.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 728)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (229.26, 108.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 232, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478)
[M::mem_pestat] mean and std.dev: (209.80, 34.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 577)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698924 reads in 208.438 CPU sec, 6.637 real sec
[M::process] read 705206 sequences (100000022 bp) in 1.517 CPU sec, 1.542 real sec...
[M::mem_process_seqs] Processed 706536 reads in 102.556 CPU sec, 3.248 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293665, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 206, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (229.30, 108.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 65, #reads = 1089099 mem = 536879782 peak = 1197772800
[M::process] read 703898 sequences (100000282 bp) in 2.332 CPU sec, 2.594 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 293402, 8, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 179, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715)
[M::mem_pestat] mean and std.dev: (197.08, 100.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (233.56, 112.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 377, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (295.33, 85.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 706578 reads in 125.614 CPU sec, 4.010 real sec
[M::mem_process_seqs] Processed 705206 reads in 82.202 CPU sec, 2.605 real sec
[M::process] read 702322 sequences (100000202 bp) in 1.739 CPU sec, 1.797 real sec...
sorting block 66, #reads = 1095472 mem = 536878724 peak = 1197772800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292917, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (235.12, 112.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702276 sequences (100000099 bp) in 1.881 CPU sec, 1.951 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292072, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.57, 115.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 319, 581)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219)
[M::mem_pestat] mean and std.dev: (311.88, 121.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1538)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 703898 reads in 121.321 CPU sec, 3.860 real sec
[M::mem_process_seqs] Processed 702322 reads in 79.268 CPU sec, 2.502 real sec
[M::process] read 701828 sequences (100000032 bp) in 1.615 CPU sec, 1.631 real sec...
sorting block 67, #reads = 1096274 mem = 536877446 peak = 1197772800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292770, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 215, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (239.68, 114.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (335, 401, 603)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139)
[M::mem_pestat] mean and std.dev: (378.70, 171.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1407)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292546, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.87, 116.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701396 sequences (100000173 bp) in 2.616 CPU sec, 2.796 real sec...
[M::mem_process_seqs] Processed 702276 reads in 120.302 CPU sec, 3.849 real sec
sorting block 68, #reads = 1094798 mem = 536878053 peak = 1197772800
[M::mem_process_seqs] Processed 701828 reads in 79.763 CPU sec, 2.528 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292534, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.48, 116.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (138, 307, 610)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1554)
[M::mem_pestat] mean and std.dev: (314.22, 221.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2026)
[M::mem_pestat] skip orientation RR
[M::process] read 701590 sequences (100000222 bp) in 2.385 CPU sec, 2.572 real sec...
[M::mem_process_seqs] Processed 701396 reads in 68.995 CPU sec, 2.274 real sec
sorting block 69, #reads = 1095289 mem = 536878566 peak = 1197772800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292204, 5, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.24, 116.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 298, 605)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395)
[M::mem_pestat] mean and std.dev: (456.23, 387.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2007)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701590 reads in 70.192 CPU sec, 2.308 real sec
[M::process] read 701810 sequences (100000100 bp) in 2.561 CPU sec, 2.772 real sec...
sorting block 70, #reads = 1093187 mem = 536880316 peak = 1197772800
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292406, 10, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (240.31, 115.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (47, 131, 643)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1835)
[M::mem_pestat] mean and std.dev: (192.75, 212.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2431)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 701682 sequences (100000218 bp) in 2.038 CPU sec, 2.245 real sec...
[M::mem_process_seqs] Processed 701810 reads in 70.332 CPU sec, 2.302 real sec
sorting block 71, #reads = 1092935 mem = 536876743 peak = 1214738432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292442, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 215, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (240.19, 114.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701476 sequences (100000236 bp) in 1.892 CPU sec, 1.972 real sec...
[M::mem_process_seqs] Processed 701682 reads in 69.364 CPU sec, 2.239 real sec
[M::process] read 700506 sequences (100000127 bp) in 1.805 CPU sec, 1.871 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291856, 8, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 183, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (206.33, 75.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.68, 116.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699706 sequences (100000291 bp) in 1.290 CPU sec, 1.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 291989, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (279, 361, 1833)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4941)
[M::mem_pestat] mean and std.dev: (751.15, 801.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6495)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (244.69, 118.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701476 reads in 123.550 CPU sec, 3.883 real sec
[M::process] read 699572 sequences (100000199 bp) in 1.338 CPU sec, 1.356 real sec...
[M::mem_process_seqs] Processed 700506 reads in 79.878 CPU sec, 2.525 real sec
sorting block 72, #reads = 1093478 mem = 536879758 peak = 1214738432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291657, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.46, 119.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 73, #reads = 1092804 mem = 536881360 peak = 1223827456
[M::process] read 700366 sequences (100000126 bp) in 1.999 CPU sec, 2.085 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291661, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.15, 119.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 469, 667)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1671)
[M::mem_pestat] mean and std.dev: (353.33, 219.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2173)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699706 reads in 124.380 CPU sec, 3.956 real sec
[M::process] read 705182 sequences (100000192 bp) in 1.492 CPU sec, 1.507 real sec...
[M::mem_process_seqs] Processed 699572 reads in 81.236 CPU sec, 2.570 real sec
sorting block 74, #reads = 1093002 mem = 536877367 peak = 1223827456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291427, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (243.68, 117.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 709296 sequences (100000163 bp) in 1.748 CPU sec, 1.799 real sec...
[M::process] read 708848 sequences (100000259 bp) in 1.380 CPU sec, 1.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292791, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (232.99, 111.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700366 reads in 137.211 CPU sec, 4.375 real sec
[M::mem_process_seqs] Processed 705182 reads in 93.664 CPU sec, 2.976 real sec
sorting block 75, #reads = 1088679 mem = 536887383 peak = 1223827456
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 294886, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (223.69, 105.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 707878 sequences (100000049 bp) in 2.025 CPU sec, 2.088 real sec...
[M::process] read 706850 sequences (100000096 bp) in 1.440 CPU sec, 1.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 294972, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 202, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (224.79, 106.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 721)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 709296 reads in 135.344 CPU sec, 4.298 real sec
[M::mem_process_seqs] Processed 708848 reads in 89.464 CPU sec, 2.844 real sec
sorting block 76, #reads = 1087586 mem = 536877505 peak = 1229254656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 294287, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 203, 292)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584)
[M::mem_pestat] mean and std.dev: (226.57, 107.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 730)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704408 sequences (100000063 bp) in 1.978 CPU sec, 2.040 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293436, 6, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (230.09, 109.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 747)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (112, 175, 253)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535)
[M::mem_pestat] mean and std.dev: (180.46, 102.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 676)
[M::mem_pestat] skip orientation RR
[M::process] read 704226 sequences (100000164 bp) in 1.510 CPU sec, 1.505 real sec...
[M::mem_process_seqs] Processed 707878 reads in 136.339 CPU sec, 4.331 real sec
sorting block 77, #reads = 1092157 mem = 536879863 peak = 1230254080
[M::mem_process_seqs] Processed 706850 reads in 89.305 CPU sec, 2.832 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292979, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (236.25, 113.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 78, #reads = 1091495 mem = 536877428 peak = 1230254080
[M::process] read 702996 sequences (100000221 bp) in 2.663 CPU sec, 2.875 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292977, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621)
[M::mem_pestat] mean and std.dev: (237.09, 114.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 778)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704408 reads in 128.786 CPU sec, 4.093 real sec
[M::mem_process_seqs] Processed 704226 reads in 84.127 CPU sec, 2.661 real sec
[M::process] read 702714 sequences (100000020 bp) in 1.929 CPU sec, 2.052 real sec...
sorting block 79, #reads = 1092659 mem = 536878612 peak = 1230254080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292534, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (239.16, 115.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702106 sequences (100000164 bp) in 1.934 CPU sec, 2.000 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292869, 7, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 275, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (241.50, 108.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.86, 116.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 702996 reads in 130.114 CPU sec, 4.164 real sec
[M::mem_process_seqs] Processed 702714 reads in 85.577 CPU sec, 2.707 real sec
sorting block 80, #reads = 1093669 mem = 536877969 peak = 1230254080
[M::process] read 701182 sequences (100000257 bp) in 1.915 CPU sec, 1.971 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292412, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.67, 117.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700750 sequences (100000036 bp) in 2.513 CPU sec, 2.732 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292461, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.59, 118.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702106 reads in 134.804 CPU sec, 4.293 real sec
[M::mem_process_seqs] Processed 701182 reads in 91.791 CPU sec, 2.911 real sec
sorting block 81, #reads = 1093457 mem = 536884425 peak = 1230254080
[M::process] read 699800 sequences (100000196 bp) in 2.620 CPU sec, 2.859 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291855, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.54, 118.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 244, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (218.00, 83.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RR
sorting block 82, #reads = 1092514 mem = 536878535 peak = 1230254080
[M::process] read 699624 sequences (100000101 bp) in 2.183 CPU sec, 2.273 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291931, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.39, 121.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 190, 924)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2486)
[M::mem_pestat] mean and std.dev: (585.40, 690.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3347)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700750 reads in 143.193 CPU sec, 4.592 real sec
[M::mem_process_seqs] Processed 699800 reads in 93.998 CPU sec, 2.978 real sec
[M::process] read 698806 sequences (100000101 bp) in 2.417 CPU sec, 2.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291351, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (249.82, 121.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 591, 1087)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2803)
[M::mem_pestat] mean and std.dev: (604.18, 412.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3661)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 83, #reads = 1093249 mem = 536878137 peak = 1230254080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291127, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.97, 120.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698136 sequences (100000241 bp) in 2.497 CPU sec, 2.681 real sec...
[M::process] read 6502 sequences (926594 bp) in 0.220 CPU sec, 0.220 real sec...
[M::mem_process_seqs] Processed 699624 reads in 142.908 CPU sec, 4.564 real sec
[M::mem_process_seqs] Processed 698806 reads in 94.939 CPU sec, 3.025 real sec
sorting block 84, #reads = 1092288 mem = 536879632 peak = 1230254080
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 2711, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (240.25, 114.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290663, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (132, 352, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1179)
[M::mem_pestat] mean and std.dev: (302.25, 229.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1528)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_process_seqs] Processed 6502 reads in 1.268 CPU sec, 0.040 real sec
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.09, 119.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 240, 1866)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5312)
[M::mem_pestat] mean and std.dev: (910.10, 1154.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7035)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698136 reads in 80.503 CPU sec, 2.652 real sec
sorting block 85, #reads = 1091080 mem = 536880638 peak = 1254457344
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 93891106
overall: 69861728256 mem 10183.921 user 337.540 sys 647.596 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 647.641 sec; CPU: 10521.471 sec
sorting block 86, #reads = 388328 mem = 191479976 peak = 1254457344
uniq header count 1
merging 87 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5198103402
output reads: 94370602
bam_mem_sort: 87 calls 32.385 user 0.898 sys 33.415 real
bam_write: 87 calls 96.252 user 2.159 sys 98.641 real
execute: 1 calls 25.722 user 0.742 sys 680.630 real
merge_files: 1 calls 25.699 user 0.714 sys 32.453 real
parse_chunk: 1382 calls 48.313 user 13.865 sys 63.033 real
read_chunk: 3733 calls 35.711 user 26.314 sys 319.812 real
sort_block: 1 calls 23.070 user 25.986 sys 303.260 real
write_chunk: 2349 calls 25.228 user 0.397 sys 31.408 real
overall: 3051376640 mem 1211.317 user 51.708 sys 680.652 real
ls: cannot access '*.cram': No such file or directory