This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_bR4-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1733.077 CPU sec, 78.340 real sec
[M::process] read 678290 sequences (100000231 bp) in 1.680 CPU sec, 1.689 real sec...
[M::process] read 677566 sequences (100000037 bp) in 2.300 CPU sec, 2.419 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282305, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 322, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (338.15, 151.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677174 sequences (100000197 bp) in 2.121 CPU sec, 3.570 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281925, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 329, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (344.02, 153.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678290 reads in 199.929 CPU sec, 8.354 real sec
[M::process] read 676654 sequences (100000255 bp) in 2.111 CPU sec, 2.471 real sec...
[M::mem_process_seqs] Processed 677566 reads in 122.303 CPU sec, 5.558 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282294, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 331, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (346.55, 154.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 0, #reads = 1080065 mem = 536879008 peak = 552534016
[M::process] read 676664 sequences (100000185 bp) in 2.679 CPU sec, 3.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281459, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 335, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (350.20, 155.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677174 reads in 188.429 CPU sec, 6.838 real sec
[M::process] read 676244 sequences (100000279 bp) in 2.445 CPU sec, 2.837 real sec...
[M::mem_process_seqs] Processed 676654 reads in 123.954 CPU sec, 4.548 real sec
sorting block 1, #reads = 1078988 mem = 536878937 peak = 679936000
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281765, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 336, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (352.09, 156.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676016 sequences (100000119 bp) in 2.595 CPU sec, 3.085 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281994, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 340, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (354.95, 157.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676664 reads in 183.043 CPU sec, 6.105 real sec
[M::mem_process_seqs] Processed 676244 reads in 122.741 CPU sec, 4.128 real sec
[M::process] read 676254 sequences (100000251 bp) in 2.554 CPU sec, 3.553 real sec...
sorting block 2, #reads = 1081209 mem = 536881599 peak = 794882048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281151, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 341, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (356.60, 157.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676014 sequences (100000155 bp) in 3.148 CPU sec, 3.870 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281120, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 341, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (356.68, 158.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676016 reads in 185.088 CPU sec, 6.274 real sec
[M::mem_process_seqs] Processed 676254 reads in 124.824 CPU sec, 4.196 real sec
sorting block 3, #reads = 1081158 mem = 536879365 peak = 794882048
[M::process] read 675550 sequences (100000030 bp) in 3.080 CPU sec, 3.711 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280928, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (359.73, 159.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281780, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (361.59, 159.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676014 reads in 189.830 CPU sec, 6.475 real sec
[M::process] read 675584 sequences (100000124 bp) in 3.157 CPU sec, 3.981 real sec...
sorting block 4, #reads = 1080805 mem = 536878478 peak = 794882048
[M::mem_process_seqs] Processed 675550 reads in 123.668 CPU sec, 4.251 real sec
[M::process] read 675250 sequences (100000274 bp) in 2.570 CPU sec, 2.926 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280913, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (361.43, 159.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675584 reads in 103.154 CPU sec, 3.702 real sec
sorting block 5, #reads = 1080441 mem = 536879015 peak = 794882048
[M::process] read 675462 sequences (100000044 bp) in 2.736 CPU sec, 3.038 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281128, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (361.95, 159.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281449, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (363.62, 160.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675188 sequences (100000285 bp) in 2.868 CPU sec, 3.184 real sec...
[M::mem_process_seqs] Processed 675250 reads in 183.392 CPU sec, 6.107 real sec
[M::mem_process_seqs] Processed 675462 reads in 123.943 CPU sec, 4.200 real sec
sorting block 6, #reads = 1079392 mem = 536877307 peak = 797753344
[M::process] read 675074 sequences (100000023 bp) in 2.608 CPU sec, 2.844 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281029, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (364.50, 160.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 7, #reads = 1080163 mem = 536877761 peak = 858685440
[M::process] read 675056 sequences (100000005 bp) in 2.919 CPU sec, 3.200 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280890, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (364.42, 161.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675188 reads in 183.665 CPU sec, 6.052 real sec
[M::mem_process_seqs] Processed 675074 reads in 125.006 CPU sec, 4.082 real sec
[M::process] read 675120 sequences (100000210 bp) in 2.780 CPU sec, 3.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281411, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (364.15, 160.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 8, #reads = 1080849 mem = 536879160 peak = 858685440
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281030, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (364.49, 161.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674862 sequences (100000090 bp) in 2.851 CPU sec, 3.147 real sec...
[M::mem_process_seqs] Processed 675056 reads in 179.999 CPU sec, 5.973 real sec
[M::mem_process_seqs] Processed 675120 reads in 121.207 CPU sec, 4.076 real sec
sorting block 9, #reads = 1080734 mem = 536878775 peak = 858685440
[M::process] read 675016 sequences (100000060 bp) in 2.805 CPU sec, 3.055 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280997, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (363.82, 160.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675348 sequences (100000272 bp) in 2.593 CPU sec, 2.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281343, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (362.54, 159.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674862 reads in 182.711 CPU sec, 5.937 real sec
[M::mem_process_seqs] Processed 675016 reads in 122.640 CPU sec, 3.959 real sec
sorting block 10, #reads = 1079457 mem = 536880007 peak = 888344576
[M::process] read 675346 sequences (100000292 bp) in 2.746 CPU sec, 2.948 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281172, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (361.17, 159.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675384 sequences (100000214 bp) in 2.839 CPU sec, 3.099 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281142, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 344, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (359.66, 159.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675348 reads in 182.549 CPU sec, 6.031 real sec
sorting block 11, #reads = 1080109 mem = 536880769 peak = 901361664
[M::mem_process_seqs] Processed 675346 reads in 124.473 CPU sec, 4.138 real sec
[M::process] read 675296 sequences (100000256 bp) in 2.685 CPU sec, 2.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281574, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 343, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (358.57, 157.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 12, #reads = 1079117 mem = 536879469 peak = 912629760
[M::process] read 675292 sequences (100000210 bp) in 2.975 CPU sec, 3.498 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281753, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 344, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (358.79, 157.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675384 reads in 183.170 CPU sec, 6.036 real sec
[M::mem_process_seqs] Processed 675296 reads in 123.200 CPU sec, 4.042 real sec
[M::process] read 674988 sequences (100000103 bp) in 2.833 CPU sec, 3.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281234, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 344, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (359.61, 158.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 13, #reads = 1079608 mem = 536884902 peak = 912629760
[M::process] read 675084 sequences (100000196 bp) in 2.862 CPU sec, 3.241 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280741, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 345, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (361.25, 158.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675292 reads in 183.822 CPU sec, 6.022 real sec
[M::mem_process_seqs] Processed 674988 reads in 124.438 CPU sec, 4.029 real sec
[M::process] read 674838 sequences (100000174 bp) in 2.712 CPU sec, 2.929 real sec...
sorting block 14, #reads = 1079724 mem = 536882020 peak = 912629760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280808, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 346, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (361.83, 159.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281226, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::process] read 674898 sequences (100000073 bp) in 2.815 CPU sec, 3.166 real sec...
[M::mem_pestat] mean and std.dev: (362.18, 158.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675084 reads in 183.573 CPU sec, 6.025 real sec
[M::mem_process_seqs] Processed 674838 reads in 122.804 CPU sec, 3.984 real sec
sorting block 15, #reads = 1080201 mem = 536879803 peak = 912629760
[M::process] read 674678 sequences (100000276 bp) in 2.951 CPU sec, 3.273 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280666, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (362.07, 159.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280903, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 347, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (362.64, 158.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674210 sequences (100000234 bp) in 2.814 CPU sec, 3.091 real sec...
[M::mem_process_seqs] Processed 674898 reads in 186.007 CPU sec, 6.041 real sec
sorting block 16, #reads = 1079947 mem = 536875644 peak = 912629760
[M::mem_process_seqs] Processed 674678 reads in 124.458 CPU sec, 3.979 real sec
[M::process] read 673912 sequences (100000074 bp) in 2.643 CPU sec, 2.830 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280651, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 350, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (364.73, 158.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 17, #reads = 1080121 mem = 536879567 peak = 912629760
[M::process] read 674484 sequences (100000166 bp) in 2.853 CPU sec, 3.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280319, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 353, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (366.60, 158.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674210 reads in 181.768 CPU sec, 5.852 real sec
[M::mem_process_seqs] Processed 673912 reads in 121.548 CPU sec, 3.864 real sec
[M::process] read 674388 sequences (100000280 bp) in 2.881 CPU sec, 3.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281141, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (361.20, 156.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 18, #reads = 1078773 mem = 536877990 peak = 912629760
[M::process] read 674422 sequences (100000216 bp) in 2.781 CPU sec, 3.001 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280911, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 347, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (361.98, 157.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674484 reads in 177.636 CPU sec, 5.719 real sec
[M::mem_process_seqs] Processed 674388 reads in 118.225 CPU sec, 3.766 real sec
[M::process] read 674360 sequences (100000088 bp) in 2.898 CPU sec, 3.055 real sec...
sorting block 19, #reads = 1079796 mem = 536881806 peak = 912629760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280813, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (360.83, 156.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280516, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 344, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (358.72, 154.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1110)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674114 sequences (100000245 bp) in 3.080 CPU sec, 3.351 real sec...
[M::mem_process_seqs] Processed 674422 reads in 174.599 CPU sec, 5.634 real sec
[M::mem_process_seqs] Processed 674360 reads in 118.076 CPU sec, 3.773 real sec
sorting block 20, #reads = 1080028 mem = 536879851 peak = 912629760
[M::process] read 674000 sequences (100000116 bp) in 2.833 CPU sec, 3.004 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280587, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 349, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (363.66, 156.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673938 sequences (100000164 bp) in 2.676 CPU sec, 2.858 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280586, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 349, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (364.20, 157.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674114 reads in 174.401 CPU sec, 5.585 real sec
sorting block 21, #reads = 1080101 mem = 536878490 peak = 912629760
[M::mem_process_seqs] Processed 674000 reads in 116.438 CPU sec, 3.691 real sec
[M::process] read 673550 sequences (100000152 bp) in 2.742 CPU sec, 2.882 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280818, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 350, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (365.30, 158.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 22, #reads = 1079275 mem = 536881175 peak = 916008960
[M::process] read 673484 sequences (100000067 bp) in 2.683 CPU sec, 2.831 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280877, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 355, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (369.97, 160.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673938 reads in 167.974 CPU sec, 5.374 real sec
[M::mem_process_seqs] Processed 673550 reads in 112.860 CPU sec, 3.561 real sec
[M::process] read 673812 sequences (100000062 bp) in 2.904 CPU sec, 3.013 real sec...
sorting block 23, #reads = 1080113 mem = 536881070 peak = 916008960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280750, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 355, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (370.90, 160.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677372 sequences (100000191 bp) in 2.602 CPU sec, 2.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280891, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 352, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (367.66, 159.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673484 reads in 165.506 CPU sec, 5.262 real sec
[M::mem_process_seqs] Processed 673812 reads in 111.116 CPU sec, 3.494 real sec
sorting block 24, #reads = 1079901 mem = 536880028 peak = 916008960
[M::process] read 677320 sequences (100000288 bp) in 2.694 CPU sec, 2.772 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282112, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 332, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (347.92, 156.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677038 sequences (100000250 bp) in 2.494 CPU sec, 2.601 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281776, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 334, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (350.01, 157.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677372 reads in 164.588 CPU sec, 5.230 real sec
[M::mem_process_seqs] Processed 677320 reads in 109.627 CPU sec, 3.458 real sec
sorting block 25, #reads = 1079462 mem = 536879913 peak = 916008960
[M::process] read 676486 sequences (100000031 bp) in 2.435 CPU sec, 2.482 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281355, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 337, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (353.31, 159.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676586 sequences (100000300 bp) in 2.465 CPU sec, 2.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282163, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 341, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (356.21, 159.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677038 reads in 161.711 CPU sec, 5.128 real sec
sorting block 26, #reads = 1081938 mem = 536880396 peak = 916008960
[M::mem_process_seqs] Processed 676486 reads in 107.625 CPU sec, 3.383 real sec
[M::process] read 676410 sequences (100000140 bp) in 2.247 CPU sec, 2.263 real sec...
sorting block 27, #reads = 1081719 mem = 536878558 peak = 916008960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280977, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 342, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (357.27, 160.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676050 sequences (100000183 bp) in 2.242 CPU sec, 2.298 real sec...
[M::process] read 675934 sequences (100000246 bp) in 1.530 CPU sec, 1.533 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281320, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 343, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (358.37, 160.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676586 reads in 158.388 CPU sec, 5.029 real sec
[M::mem_process_seqs] Processed 676410 reads in 103.352 CPU sec, 3.284 real sec
sorting block 28, #reads = 1080718 mem = 536878769 peak = 916008960
[M::process] read 675760 sequences (100000070 bp) in 2.376 CPU sec, 2.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281174, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 346, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (361.61, 161.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675676 sequences (100000168 bp) in 1.990 CPU sec, 1.991 real sec...
[M::mem_process_seqs] Processed 675934 reads in 86.505 CPU sec, 2.703 real sec
sorting block 29, #reads = 1081802 mem = 536879605 peak = 916008960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281556, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 344, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (360.19, 161.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280717, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 347, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (361.60, 160.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676050 reads in 241.477 CPU sec, 7.632 real sec
[M::process] read 675356 sequences (100000268 bp) in 2.107 CPU sec, 2.118 real sec...
[M::mem_process_seqs] Processed 675760 reads in 120.601 CPU sec, 3.819 real sec
sorting block 30, #reads = 1080636 mem = 536879744 peak = 916008960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281288, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (363.55, 161.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (283, 283, 1621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4297)
[M::mem_pestat] mean and std.dev: (657.60, 635.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5635)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675374 sequences (100000289 bp) in 2.708 CPU sec, 2.796 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280929, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (364.72, 161.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675146 sequences (100000205 bp) in 1.580 CPU sec, 1.577 real sec...
[M::mem_process_seqs] Processed 675676 reads in 150.067 CPU sec, 4.741 real sec
[M::mem_process_seqs] Processed 675356 reads in 99.737 CPU sec, 3.145 real sec
sorting block 31, #reads = 1080843 mem = 536876191 peak = 916008960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281155, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (364.56, 161.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675276 sequences (100000171 bp) in 2.486 CPU sec, 2.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281549, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (366.41, 162.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675374 reads in 147.507 CPU sec, 4.655 real sec
[M::mem_process_seqs] Processed 675146 reads in 96.297 CPU sec, 3.016 real sec
[M::process] read 674952 sequences (100000035 bp) in 2.261 CPU sec, 2.274 real sec...
sorting block 32, #reads = 1080972 mem = 536877140 peak = 916008960
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281347, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (366.30, 162.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674948 sequences (100000031 bp) in 2.704 CPU sec, 2.756 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280755, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (367.35, 162.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675276 reads in 148.845 CPU sec, 4.711 real sec
sorting block 33, #reads = 1081672 mem = 536877467 peak = 916008960
[M::mem_process_seqs] Processed 674952 reads in 100.372 CPU sec, 3.146 real sec
[M::process] read 674788 sequences (100000204 bp) in 2.131 CPU sec, 2.159 real sec...
sorting block 34, #reads = 1081322 mem = 536877121 peak = 954982400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281180, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 354, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (368.72, 163.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675150 sequences (100000125 bp) in 2.204 CPU sec, 2.266 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280801, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 674914 sequences (100000047 bp) in 1.590 CPU sec, 1.587 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (243, 354, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (369.26, 163.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674948 reads in 151.268 CPU sec, 4.793 real sec
[M::mem_process_seqs] Processed 674788 reads in 100.225 CPU sec, 3.173 real sec
sorting block 35, #reads = 1080461 mem = 536878629 peak = 954982400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281227, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (367.78, 163.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675194 sequences (100000042 bp) in 2.853 CPU sec, 2.921 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280587, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (366.16, 162.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675150 reads in 151.082 CPU sec, 4.769 real sec
[M::mem_process_seqs] Processed 674914 reads in 100.400 CPU sec, 3.149 real sec
[M::process] read 675338 sequences (100000143 bp) in 2.247 CPU sec, 2.270 real sec...
sorting block 36, #reads = 1080447 mem = 536879395 peak = 954982400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281118, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 346, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (362.56, 160.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675314 sequences (100000150 bp) in 2.653 CPU sec, 2.711 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281421, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (363.93, 162.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675194 reads in 150.932 CPU sec, 4.773 real sec
[M::mem_process_seqs] Processed 675338 reads in 100.692 CPU sec, 3.156 real sec
[M::process] read 675238 sequences (100000224 bp) in 2.422 CPU sec, 2.431 real sec...
sorting block 37, #reads = 1079709 mem = 536881384 peak = 954982400
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281056, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (363.38, 162.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675300 sequences (100000235 bp) in 2.470 CPU sec, 2.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281535, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (364.17, 162.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675314 reads in 150.139 CPU sec, 4.751 real sec
sorting block 38, #reads = 1079543 mem = 536877351 peak = 954982400
[M::mem_process_seqs] Processed 675238 reads in 99.159 CPU sec, 3.114 real sec
[M::process] read 675396 sequences (100000289 bp) in 2.428 CPU sec, 2.460 real sec...
sorting block 39, #reads = 1079900 mem = 536882338 peak = 957202432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281149, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (362.87, 161.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675192 sequences (100000199 bp) in 2.608 CPU sec, 2.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281245, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (361.32, 161.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675300 reads in 148.686 CPU sec, 4.701 real sec
[M::process] read 675054 sequences (100000166 bp) in 1.748 CPU sec, 1.764 real sec...
[M::mem_process_seqs] Processed 675396 reads in 98.535 CPU sec, 3.098 real sec
sorting block 40, #reads = 1080547 mem = 536881729 peak = 957202432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282090, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (361.43, 160.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674852 sequences (100000005 bp) in 2.251 CPU sec, 2.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280710, 10, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (362.25, 160.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (316, 316, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (278, 373)
[M::mem_pestat] mean and std.dev: (321.43, 8.58)
[M::mem_pestat] low and high boundaries for proper pairs: (259, 392)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675192 reads in 144.924 CPU sec, 4.587 real sec
[M::mem_process_seqs] Processed 675054 reads in 96.466 CPU sec, 3.029 real sec
[M::process] read 675190 sequences (100000083 bp) in 2.330 CPU sec, 2.346 real sec...
sorting block 41, #reads = 1080397 mem = 536878188 peak = 957202432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281547, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (363.65, 160.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674734 sequences (100000037 bp) in 2.674 CPU sec, 2.725 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281063, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 345, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (361.97, 161.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674852 reads in 149.090 CPU sec, 4.715 real sec
[M::process] read 674868 sequences (100000134 bp) in 1.774 CPU sec, 1.780 real sec...
[M::mem_process_seqs] Processed 675190 reads in 100.818 CPU sec, 3.175 real sec
sorting block 42, #reads = 1080487 mem = 536877037 peak = 957202432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280987, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 347, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (362.52, 159.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674686 sequences (100000014 bp) in 2.478 CPU sec, 2.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280893, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (364.45, 161.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 197, 2230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6296)
[M::mem_pestat] mean and std.dev: (1296.45, 1930.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9019)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674734 reads in 149.185 CPU sec, 4.716 real sec
sorting block 43, #reads = 1080536 mem = 536877885 peak = 957202432
[M::mem_process_seqs] Processed 674868 reads in 99.799 CPU sec, 3.130 real sec
[M::process] read 674700 sequences (100000243 bp) in 2.397 CPU sec, 2.416 real sec...
sorting block 44, #reads = 1079445 mem = 536881033 peak = 1028521984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281122, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (366.55, 161.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674522 sequences (100000154 bp) in 2.201 CPU sec, 2.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280919, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (365.08, 161.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674530 sequences (100000063 bp) in 1.630 CPU sec, 1.627 real sec...
[M::mem_process_seqs] Processed 674686 reads in 148.117 CPU sec, 4.691 real sec
[M::mem_process_seqs] Processed 674700 reads in 97.854 CPU sec, 3.093 real sec
sorting block 45, #reads = 1078027 mem = 536879585 peak = 1028521984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281022, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (367.79, 161.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674264 sequences (100000257 bp) in 2.327 CPU sec, 2.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280975, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (365.04, 160.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674522 reads in 145.573 CPU sec, 4.604 real sec
[M::process] read 674352 sequences (100000150 bp) in 1.810 CPU sec, 1.803 real sec...
[M::mem_process_seqs] Processed 674530 reads in 97.213 CPU sec, 3.059 real sec
sorting block 46, #reads = 1079161 mem = 536877783 peak = 1028521984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280818, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (364.45, 159.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673978 sequences (100000181 bp) in 2.243 CPU sec, 2.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280783, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 350, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (365.83, 160.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674098 sequences (100000195 bp) in 1.640 CPU sec, 1.641 real sec...
[M::mem_process_seqs] Processed 674264 reads in 147.261 CPU sec, 4.662 real sec
sorting block 47, #reads = 1079862 mem = 536880403 peak = 1028521984
[M::mem_process_seqs] Processed 674352 reads in 98.614 CPU sec, 3.121 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280570, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 352, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (367.35, 160.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673840 sequences (100000025 bp) in 2.153 CPU sec, 2.229 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280959, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 353, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::process] read 673722 sequences (100000212 bp) in 1.620 CPU sec, 1.621 real sec...
[M::mem_pestat] mean and std.dev: (368.59, 161.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673978 reads in 148.383 CPU sec, 4.699 real sec
sorting block 48, #reads = 1079652 mem = 536880346 peak = 1028521984
[M::mem_process_seqs] Processed 674098 reads in 97.931 CPU sec, 3.104 real sec
sorting block 49, #reads = 1078708 mem = 536880641 peak = 1040637952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281188, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 357, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (372.53, 162.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676626 sequences (100000110 bp) in 2.273 CPU sec, 2.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280813, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677626 sequences (100000264 bp) in 1.670 CPU sec, 1.671 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (251, 360, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (375.15, 164.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673840 reads in 149.130 CPU sec, 4.718 real sec
[M::mem_process_seqs] Processed 673722 reads in 100.174 CPU sec, 3.172 real sec
sorting block 50, #reads = 1079246 mem = 536880492 peak = 1040637952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281886, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 338, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (354.18, 158.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677192 sequences (100000083 bp) in 2.538 CPU sec, 2.591 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282025, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (346.68, 155.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676626 reads in 153.087 CPU sec, 4.830 real sec
[M::process] read 676990 sequences (100000013 bp) in 1.989 CPU sec, 1.982 real sec...
[M::mem_process_seqs] Processed 677626 reads in 103.233 CPU sec, 3.245 real sec
sorting block 51, #reads = 1078956 mem = 536884982 peak = 1040637952
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282253, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 333, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (348.43, 155.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676782 sequences (100000051 bp) in 2.625 CPU sec, 2.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281778, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 333, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (348.52, 154.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677192 reads in 154.384 CPU sec, 4.868 real sec
[M::process] read 676530 sequences (100000118 bp) in 2.237 CPU sec, 2.240 real sec...
sorting block 52, #reads = 1080912 mem = 536878945 peak = 1040637952
[M::mem_process_seqs] Processed 676990 reads in 103.153 CPU sec, 3.234 real sec
[M::process] read 676328 sequences (100000165 bp) in 2.525 CPU sec, 2.570 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281232, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 339, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (353.90, 156.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676530 reads in 84.732 CPU sec, 2.652 real sec
sorting block 53, #reads = 1081495 mem = 536883218 peak = 1040637952
[M::process] read 676192 sequences (100000207 bp) in 2.284 CPU sec, 2.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281666, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 336, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (350.45, 154.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281266, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 340, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (354.24, 156.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676782 reads in 232.598 CPU sec, 7.341 real sec
[M::process] read 676006 sequences (100000162 bp) in 2.007 CPU sec, 2.029 real sec...
[M::mem_process_seqs] Processed 676328 reads in 115.296 CPU sec, 3.631 real sec
sorting block 54, #reads = 1080788 mem = 536882654 peak = 1041969152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280822, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 341, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (355.26, 156.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675720 sequences (100000166 bp) in 2.398 CPU sec, 2.477 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281442, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 342, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (356.03, 156.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675652 sequences (100000136 bp) in 1.580 CPU sec, 1.584 real sec...
[M::mem_process_seqs] Processed 676192 reads in 148.226 CPU sec, 4.696 real sec
sorting block 55, #reads = 1079700 mem = 536883639 peak = 1041969152
[M::mem_process_seqs] Processed 676006 reads in 97.806 CPU sec, 3.087 real sec
sorting block 56, #reads = 1079652 mem = 536880073 peak = 1041969152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281108, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 343, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (356.46, 155.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675492 sequences (100000257 bp) in 2.776 CPU sec, 2.817 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281546, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 343, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (356.45, 155.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (345, 345, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (345, 345)
[M::mem_pestat] mean and std.dev: (345.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (345, 345)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675720 reads in 146.513 CPU sec, 4.620 real sec
[M::mem_process_seqs] Processed 675652 reads in 97.326 CPU sec, 3.055 real sec
[M::process] read 675514 sequences (100000155 bp) in 2.294 CPU sec, 2.299 real sec...
sorting block 57, #reads = 1080189 mem = 536879614 peak = 1041969152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281677, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 345, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (358.52, 156.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675162 sequences (100000104 bp) in 2.499 CPU sec, 2.577 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281330, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (360.57, 157.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675492 reads in 146.938 CPU sec, 4.642 real sec
[M::mem_process_seqs] Processed 675514 reads in 97.076 CPU sec, 3.048 real sec
[M::process] read 675004 sequences (100000241 bp) in 2.392 CPU sec, 2.412 real sec...
sorting block 58, #reads = 1079192 mem = 536877806 peak = 1041969152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281198, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (361.64, 158.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674812 sequences (100000007 bp) in 2.687 CPU sec, 2.748 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280837, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (364.11, 158.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675162 reads in 148.445 CPU sec, 4.694 real sec
[M::mem_process_seqs] Processed 675004 reads in 98.175 CPU sec, 3.080 real sec
sorting block 59, #reads = 1079712 mem = 536880765 peak = 1041969152
[M::process] read 675114 sequences (100000167 bp) in 2.524 CPU sec, 2.547 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280772, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (365.09, 159.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674794 sequences (100000231 bp) in 2.514 CPU sec, 2.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280645, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 351, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (365.00, 159.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674812 reads in 150.243 CPU sec, 4.757 real sec
sorting block 60, #reads = 1079245 mem = 536878236 peak = 1041969152
[M::mem_process_seqs] Processed 675114 reads in 99.451 CPU sec, 3.125 real sec
[M::process] read 674848 sequences (100000053 bp) in 2.516 CPU sec, 2.552 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281167, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 351, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (364.45, 158.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 61, #reads = 1079732 mem = 536881681 peak = 1041969152
[M::process] read 675054 sequences (100000202 bp) in 2.659 CPU sec, 2.723 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281016, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 352, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (366.20, 160.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674794 reads in 147.582 CPU sec, 4.674 real sec
[M::mem_process_seqs] Processed 674848 reads in 98.346 CPU sec, 3.085 real sec
[M::process] read 674856 sequences (100000082 bp) in 2.556 CPU sec, 2.596 real sec...
sorting block 62, #reads = 1080396 mem = 536878459 peak = 1041969152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280864, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (363.85, 159.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675124 sequences (100000143 bp) in 2.595 CPU sec, 2.643 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280810, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (362.88, 159.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675054 reads in 149.342 CPU sec, 4.729 real sec
[M::mem_process_seqs] Processed 674856 reads in 99.734 CPU sec, 3.138 real sec
sorting block 63, #reads = 1080000 mem = 536879340 peak = 1041969152
[M::process] read 674990 sequences (100000184 bp) in 2.659 CPU sec, 2.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280790, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (362.23, 158.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675060 sequences (100000134 bp) in 2.032 CPU sec, 2.081 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281359, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (361.07, 158.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675072 sequences (100000248 bp) in 1.530 CPU sec, 1.525 real sec...
[M::mem_process_seqs] Processed 675124 reads in 152.541 CPU sec, 4.836 real sec
sorting block 64, #reads = 1079694 mem = 536878789 peak = 1041969152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281261, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (361.33, 159.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675144 sequences (100000178 bp) in 2.191 CPU sec, 2.198 real sec...
[M::mem_process_seqs] Processed 675060 reads in 87.082 CPU sec, 2.746 real sec
[M::mem_process_seqs] Processed 674990 reads in 204.904 CPU sec, 6.473 real sec
sorting block 65, #reads = 1079459 mem = 536877787 peak = 1041969152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281292, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (362.73, 159.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674910 sequences (100000036 bp) in 2.617 CPU sec, 2.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280552, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (361.43, 159.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675072 reads in 169.645 CPU sec, 5.356 real sec
[M::mem_process_seqs] Processed 675144 reads in 101.312 CPU sec, 3.183 real sec
sorting block 66, #reads = 1080221 mem = 536878468 peak = 1041969152
[M::process] read 674758 sequences (100000141 bp) in 2.373 CPU sec, 2.385 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280400, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 344, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (360.19, 158.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674850 sequences (100000156 bp) in 2.463 CPU sec, 2.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280640, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 346, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (361.66, 158.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674910 reads in 153.385 CPU sec, 4.838 real sec
[M::process] read 674758 sequences (100000132 bp) in 2.147 CPU sec, 2.159 real sec...
sorting block 67, #reads = 1079149 mem = 536879361 peak = 1041969152
[M::mem_process_seqs] Processed 674758 reads in 100.339 CPU sec, 3.150 real sec
sorting block 68, #reads = 1079441 mem = 536878672 peak = 1056595968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280293, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (363.65, 159.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674662 sequences (100000042 bp) in 2.024 CPU sec, 2.077 real sec...
[M::process] read 674548 sequences (100000124 bp) in 1.570 CPU sec, 1.573 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280739, 4, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 908, 908)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2408)
[M::mem_pestat] mean and std.dev: (511.56, 369.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3158)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (364.04, 160.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674850 reads in 152.277 CPU sec, 4.829 real sec
[M::mem_process_seqs] Processed 674758 reads in 99.139 CPU sec, 3.143 real sec
sorting block 69, #reads = 1079561 mem = 536877114 peak = 1056595968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280889, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (364.58, 160.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674558 sequences (100000057 bp) in 2.455 CPU sec, 2.596 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280673, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (365.12, 160.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674662 reads in 150.169 CPU sec, 4.750 real sec
[M::mem_process_seqs] Processed 674548 reads in 99.251 CPU sec, 3.115 real sec
[M::process] read 674720 sequences (100000293 bp) in 2.319 CPU sec, 2.328 real sec...
sorting block 70, #reads = 1079528 mem = 536879842 peak = 1056595968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280654, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 350, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (365.45, 160.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674436 sequences (100000120 bp) in 2.651 CPU sec, 2.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281011, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 349, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (364.89, 160.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674558 reads in 149.496 CPU sec, 4.723 real sec
[M::mem_process_seqs] Processed 674720 reads in 97.110 CPU sec, 3.038 real sec
[M::process] read 674062 sequences (100000001 bp) in 2.353 CPU sec, 2.374 real sec...
sorting block 71, #reads = 1079802 mem = 536878423 peak = 1056595968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280849, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 353, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (368.91, 161.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674124 sequences (100000092 bp) in 2.480 CPU sec, 2.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280742, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (368.27, 161.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674436 reads in 148.082 CPU sec, 4.690 real sec
[M::mem_process_seqs] Processed 674062 reads in 97.571 CPU sec, 3.063 real sec
sorting block 72, #reads = 1080003 mem = 536878099 peak = 1056595968
[M::process] read 673868 sequences (100000260 bp) in 2.437 CPU sec, 2.472 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280951, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 355, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (369.93, 161.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (379, 379, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (379, 379)
[M::mem_pestat] mean and std.dev: (379.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (379, 379)
[M::mem_pestat] skip orientation RR
sorting block 73, #reads = 1079882 mem = 536880594 peak = 1056595968
[M::process] read 673920 sequences (100000092 bp) in 2.608 CPU sec, 2.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280422, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 356, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (371.64, 163.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674124 reads in 146.163 CPU sec, 4.624 real sec
[M::mem_process_seqs] Processed 673868 reads in 97.513 CPU sec, 3.057 real sec
[M::process] read 673770 sequences (100000220 bp) in 2.510 CPU sec, 2.542 real sec...
sorting block 74, #reads = 1079949 mem = 536876156 peak = 1056595968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280690, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 354, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (370.19, 161.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673738 sequences (100000185 bp) in 2.873 CPU sec, 3.005 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280482, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 355, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (370.71, 161.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673920 reads in 147.480 CPU sec, 4.682 real sec
[M::mem_process_seqs] Processed 673770 reads in 97.226 CPU sec, 3.062 real sec
sorting block 75, #reads = 1079071 mem = 536878670 peak = 1056595968
[M::process] read 677392 sequences (100000148 bp) in 2.422 CPU sec, 2.518 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280801, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 356, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (372.03, 163.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677390 sequences (100000137 bp) in 2.017 CPU sec, 2.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282198, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 333, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (349.66, 158.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673738 reads in 149.507 CPU sec, 4.721 real sec
[M::process] read 677292 sequences (100000136 bp) in 1.561 CPU sec, 1.568 real sec...
[M::mem_process_seqs] Processed 677392 reads in 100.051 CPU sec, 3.144 real sec
sorting block 76, #reads = 1079925 mem = 536882599 peak = 1056595968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281840, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 333, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (350.78, 159.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676946 sequences (100000066 bp) in 2.576 CPU sec, 2.635 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281301, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (353.08, 161.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677390 reads in 149.256 CPU sec, 4.718 real sec
sorting block 77, #reads = 1081007 mem = 536878312 peak = 1056595968
[M::mem_process_seqs] Processed 677292 reads in 98.528 CPU sec, 3.090 real sec
[M::process] read 676738 sequences (100000028 bp) in 2.311 CPU sec, 2.327 real sec...
sorting block 78, #reads = 1082070 mem = 536880514 peak = 1056595968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281248, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 338, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (354.86, 161.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676378 sequences (100000279 bp) in 2.584 CPU sec, 2.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281398, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 339, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (355.64, 161.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676946 reads in 147.746 CPU sec, 4.658 real sec
[M::mem_process_seqs] Processed 676738 reads in 98.565 CPU sec, 3.095 real sec
[M::process] read 676408 sequences (100000228 bp) in 2.287 CPU sec, 2.296 real sec...
sorting block 79, #reads = 1081939 mem = 536877974 peak = 1056595968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281148, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 342, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (357.99, 160.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676174 sequences (100000060 bp) in 2.545 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280665, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (358.41, 161.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676378 reads in 150.885 CPU sec, 4.776 real sec
[M::mem_process_seqs] Processed 676408 reads in 97.757 CPU sec, 3.068 real sec
[M::process] read 676380 sequences (100000153 bp) in 2.358 CPU sec, 2.382 real sec...
sorting block 80, #reads = 1081698 mem = 536876996 peak = 1056595968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281238, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 344, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (359.56, 161.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675940 sequences (100000269 bp) in 2.665 CPU sec, 2.719 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281229, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 343, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (360.31, 162.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676174 reads in 150.053 CPU sec, 4.744 real sec
[M::mem_process_seqs] Processed 676380 reads in 98.927 CPU sec, 3.107 real sec
[M::process] read 675800 sequences (100000023 bp) in 2.390 CPU sec, 2.408 real sec...
sorting block 81, #reads = 1081423 mem = 536878497 peak = 1056595968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281069, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (361.82, 161.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675734 sequences (100000020 bp) in 2.516 CPU sec, 2.569 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281315, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (361.34, 161.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675940 reads in 149.128 CPU sec, 4.721 real sec
sorting block 82, #reads = 1081630 mem = 536878626 peak = 1056595968
[M::mem_process_seqs] Processed 675800 reads in 98.810 CPU sec, 3.101 real sec
[M::process] read 675682 sequences (100000199 bp) in 2.417 CPU sec, 2.441 real sec...
sorting block 83, #reads = 1081436 mem = 536878710 peak = 1056595968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281144, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (362.34, 160.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675758 sequences (100000119 bp) in 2.671 CPU sec, 2.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280804, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (361.59, 159.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675734 reads in 153.311 CPU sec, 4.855 real sec
[M::process] read 676272 sequences (100000136 bp) in 1.912 CPU sec, 1.937 real sec...
[M::mem_process_seqs] Processed 675682 reads in 101.229 CPU sec, 3.181 real sec
sorting block 84, #reads = 1080123 mem = 536878179 peak = 1056595968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280759, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (361.47, 160.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675162 sequences (100000277 bp) in 2.507 CPU sec, 2.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280940, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 341, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (357.69, 160.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675758 reads in 149.946 CPU sec, 4.746 real sec
[M::mem_process_seqs] Processed 676272 reads in 98.525 CPU sec, 3.089 real sec
[M::process] read 675148 sequences (100000204 bp) in 2.403 CPU sec, 2.418 real sec...
sorting block 85, #reads = 1080302 mem = 536878832 peak = 1056595968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280909, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (365.37, 161.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675082 sequences (100000205 bp) in 2.312 CPU sec, 2.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280963, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (365.32, 160.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675022 sequences (100000284 bp) in 1.590 CPU sec, 1.583 real sec...
[M::mem_process_seqs] Processed 675162 reads in 150.698 CPU sec, 4.767 real sec
sorting block 86, #reads = 1080446 mem = 536879859 peak = 1056595968
[M::mem_process_seqs] Processed 675148 reads in 99.625 CPU sec, 3.151 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281034, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (364.97, 160.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675040 sequences (100000278 bp) in 2.522 CPU sec, 2.566 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280763, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (365.40, 160.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675082 reads in 152.362 CPU sec, 4.801 real sec
[M::process] read 675126 sequences (100000166 bp) in 2.246 CPU sec, 2.258 real sec...
sorting block 87, #reads = 1080929 mem = 536879538 peak = 1056595968
[M::mem_process_seqs] Processed 675022 reads in 99.645 CPU sec, 3.125 real sec
sorting block 88, #reads = 1079403 mem = 536879703 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280672, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 349, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (363.74, 159.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675032 sequences (100000116 bp) in 2.235 CPU sec, 2.304 real sec...
[M::process] read 675272 sequences (100000055 bp) in 1.600 CPU sec, 1.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281145, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (362.15, 158.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675040 reads in 152.184 CPU sec, 4.824 real sec
[M::mem_process_seqs] Processed 675126 reads in 98.791 CPU sec, 3.140 real sec
sorting block 89, #reads = 1079835 mem = 536879276 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280938, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 346, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (361.22, 158.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675196 sequences (100000262 bp) in 2.578 CPU sec, 2.629 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280555, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (361.77, 159.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675164 sequences (100000128 bp) in 1.760 CPU sec, 1.763 real sec...
[M::mem_process_seqs] Processed 675032 reads in 153.291 CPU sec, 4.841 real sec
[M::mem_process_seqs] Processed 675272 reads in 101.664 CPU sec, 3.198 real sec
sorting block 90, #reads = 1079953 mem = 536880728 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280569, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 347, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (362.44, 159.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675180 sequences (100000257 bp) in 2.820 CPU sec, 2.884 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280729, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (362.01, 159.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675196 reads in 155.246 CPU sec, 4.892 real sec
[M::process] read 675152 sequences (100000120 bp) in 2.243 CPU sec, 2.246 real sec...
[M::mem_process_seqs] Processed 675164 reads in 103.033 CPU sec, 3.237 real sec
sorting block 91, #reads = 1078672 mem = 536880886 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280453, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (361.19, 159.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675090 sequences (100000275 bp) in 2.535 CPU sec, 2.607 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281120, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (360.27, 158.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675180 reads in 153.980 CPU sec, 4.871 real sec
sorting block 92, #reads = 1079423 mem = 536879247 peak = 1102123008
[M::mem_process_seqs] Processed 675152 reads in 100.893 CPU sec, 3.162 real sec
[M::process] read 675186 sequences (100000087 bp) in 2.353 CPU sec, 2.354 real sec...
sorting block 93, #reads = 1078927 mem = 536881671 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280439, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 345, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (360.76, 158.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674720 sequences (100000106 bp) in 2.680 CPU sec, 2.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281263, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 346, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (361.81, 159.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675090 reads in 153.201 CPU sec, 4.840 real sec
[M::mem_process_seqs] Processed 675186 reads in 100.741 CPU sec, 3.155 real sec
[M::process] read 674850 sequences (100000223 bp) in 2.359 CPU sec, 2.362 real sec...
sorting block 94, #reads = 1078756 mem = 536879067 peak = 1102123008
[M::process] read 674482 sequences (100000170 bp) in 2.603 CPU sec, 2.668 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280569, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 345, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (360.31, 157.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674850 reads in 86.844 CPU sec, 2.720 real sec
[M::process] read 674614 sequences (100000233 bp) in 2.296 CPU sec, 2.321 real sec...
sorting block 95, #reads = 1078971 mem = 536878153 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280236, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 345, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (360.37, 157.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280356, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 348, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (362.84, 157.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674720 reads in 239.130 CPU sec, 7.552 real sec
[M::process] read 674630 sequences (100000149 bp) in 1.836 CPU sec, 1.849 real sec...
[M::mem_process_seqs] Processed 674482 reads in 120.457 CPU sec, 3.791 real sec
sorting block 96, #reads = 1079177 mem = 536878194 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280841, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 348, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (363.17, 158.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674368 sequences (100000052 bp) in 2.853 CPU sec, 2.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280901, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (361.09, 157.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674614 reads in 150.467 CPU sec, 4.754 real sec
[M::mem_process_seqs] Processed 674630 reads in 98.827 CPU sec, 3.099 real sec
[M::process] read 674582 sequences (100000233 bp) in 2.333 CPU sec, 2.370 real sec...
sorting block 97, #reads = 1079032 mem = 536879419 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280399, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 345, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (360.26, 155.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674208 sequences (100000097 bp) in 2.665 CPU sec, 2.722 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281281, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 346, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (360.14, 155.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674368 reads in 146.990 CPU sec, 4.647 real sec
[M::mem_process_seqs] Processed 674582 reads in 96.847 CPU sec, 3.040 real sec
sorting block 98, #reads = 1079301 mem = 536877697 peak = 1102123008
[M::process] read 673998 sequences (100000027 bp) in 2.409 CPU sec, 2.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280386, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 350, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (364.26, 157.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673886 sequences (100000249 bp) in 2.444 CPU sec, 2.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280058, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 352, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (366.25, 158.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674208 reads in 145.155 CPU sec, 4.592 real sec
sorting block 99, #reads = 1077974 mem = 536877184 peak = 1102123008
[M::mem_process_seqs] Processed 673998 reads in 97.213 CPU sec, 3.051 real sec
[M::process] read 673822 sequences (100000139 bp) in 2.286 CPU sec, 2.309 real sec...
sorting block 100, #reads = 1078339 mem = 536878744 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281678, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 355, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (368.77, 159.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674856 sequences (100000233 bp) in 2.004 CPU sec, 2.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281325, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 356, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (371.36, 161.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680814 sequences (100000293 bp) in 1.590 CPU sec, 1.582 real sec...
[M::mem_process_seqs] Processed 673886 reads in 143.616 CPU sec, 4.544 real sec
[M::mem_process_seqs] Processed 673822 reads in 95.570 CPU sec, 3.015 real sec
sorting block 101, #reads = 1079123 mem = 536881360 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281088, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (367.37, 163.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679652 sequences (100000084 bp) in 2.593 CPU sec, 2.656 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283147, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 312, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (335.85, 160.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674856 reads in 155.169 CPU sec, 4.889 real sec
[M::process] read 678994 sequences (100000301 bp) in 2.283 CPU sec, 2.287 real sec...
[M::mem_process_seqs] Processed 680814 reads in 104.827 CPU sec, 3.290 real sec
sorting block 102, #reads = 1078372 mem = 536880982 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282256, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 320, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (342.52, 162.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678580 sequences (100000109 bp) in 2.566 CPU sec, 2.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282415, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 324, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (345.03, 161.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679652 reads in 155.460 CPU sec, 4.916 real sec
[M::process] read 678476 sequences (100000237 bp) in 2.262 CPU sec, 2.275 real sec...
[M::mem_process_seqs] Processed 678994 reads in 101.847 CPU sec, 3.191 real sec
sorting block 103, #reads = 1080597 mem = 536880922 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282389, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 328, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (348.12, 163.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678018 sequences (100000079 bp) in 2.543 CPU sec, 2.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 282722, 2, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (655, 1064, 1064)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1882)
[M::mem_pestat] mean and std.dev: (824.09, 434.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2562)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (348.65, 161.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 678580 reads in 157.478 CPU sec, 4.968 real sec
[M::process] read 677842 sequences (100000095 bp) in 2.297 CPU sec, 2.296 real sec...
sorting block 104, #reads = 1080732 mem = 536878851 peak = 1102123008
[M::mem_process_seqs] Processed 678476 reads in 103.895 CPU sec, 3.256 real sec
sorting block 105, #reads = 1080901 mem = 536880171 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282209, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (349.90, 161.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677398 sequences (100000192 bp) in 2.811 CPU sec, 2.867 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281477, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (352.23, 162.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678018 reads in 161.494 CPU sec, 5.095 real sec
[M::process] read 677190 sequences (100000091 bp) in 2.292 CPU sec, 2.292 real sec...
[M::mem_process_seqs] Processed 677842 reads in 106.739 CPU sec, 3.346 real sec
sorting block 106, #reads = 1079428 mem = 536879020 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281992, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (355.45, 163.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677070 sequences (100000223 bp) in 2.539 CPU sec, 2.593 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282386, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 339, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (356.37, 163.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676656 sequences (100000092 bp) in 1.950 CPU sec, 1.956 real sec...
[M::mem_process_seqs] Processed 677398 reads in 165.885 CPU sec, 5.246 real sec
[M::mem_process_seqs] Processed 677190 reads in 113.116 CPU sec, 3.566 real sec
sorting block 107, #reads = 1078410 mem = 536881665 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281693, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 342, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (359.54, 164.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676278 sequences (100000289 bp) in 2.275 CPU sec, 2.339 real sec...
[M::process] read 676298 sequences (100000155 bp) in 1.670 CPU sec, 1.663 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281765, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 344, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (361.31, 165.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677070 reads in 156.495 CPU sec, 4.952 real sec
sorting block 108, #reads = 1078555 mem = 536878573 peak = 1102123008
[M::mem_process_seqs] Processed 676656 reads in 101.392 CPU sec, 3.226 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281933, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 346, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (362.17, 164.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676138 sequences (100000114 bp) in 2.783 CPU sec, 3.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281847, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 347, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (363.27, 164.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676278 reads in 153.855 CPU sec, 4.870 real sec
sorting block 109, #reads = 1079076 mem = 536876822 peak = 1102123008
[M::mem_process_seqs] Processed 676298 reads in 102.833 CPU sec, 3.235 real sec
[M::process] read 676336 sequences (100000104 bp) in 2.539 CPU sec, 2.557 real sec...
sorting block 110, #reads = 1078727 mem = 536878994 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281295, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 348, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (364.46, 164.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675912 sequences (100000142 bp) in 2.906 CPU sec, 2.958 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282100, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 346, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (362.05, 162.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676138 reads in 156.594 CPU sec, 4.952 real sec
[M::mem_process_seqs] Processed 676336 reads in 103.928 CPU sec, 3.259 real sec
[M::process] read 675840 sequences (100000059 bp) in 2.614 CPU sec, 2.637 real sec...
sorting block 111, #reads = 1078219 mem = 536880570 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282225, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 351, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (366.35, 164.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675356 sequences (100000038 bp) in 2.498 CPU sec, 2.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281167, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 351, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (366.38, 164.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675912 reads in 161.123 CPU sec, 5.097 real sec
[M::mem_process_seqs] Processed 675840 reads in 108.428 CPU sec, 3.401 real sec
[M::process] read 675244 sequences (100000014 bp) in 2.381 CPU sec, 2.401 real sec...
sorting block 112, #reads = 1077395 mem = 536879013 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281201, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 352, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (367.86, 164.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675682 sequences (100000079 bp) in 2.774 CPU sec, 2.816 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281199, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 351, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (366.91, 163.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675356 reads in 162.418 CPU sec, 5.128 real sec
[M::process] read 675248 sequences (100000131 bp) in 2.308 CPU sec, 2.320 real sec...
[M::mem_process_seqs] Processed 675244 reads in 107.557 CPU sec, 3.370 real sec
sorting block 113, #reads = 1077254 mem = 536878694 peak = 1102123008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281276, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (363.35, 161.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675410 sequences (100000286 bp) in 2.571 CPU sec, 2.622 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280671, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (365.36, 162.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675682 reads in 158.603 CPU sec, 5.003 real sec
sorting block 114, #reads = 1076946 mem = 536879880 peak = 1102123008
[M::process] read 675114 sequences (100000065 bp) in 2.283 CPU sec, 2.291 real sec...
[M::mem_process_seqs] Processed 675248 reads in 104.747 CPU sec, 3.290 real sec
sorting block 115, #reads = 1077040 mem = 536877764 peak = 1107939328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281164, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 349, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (364.72, 162.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675282 sequences (100000086 bp) in 2.815 CPU sec, 2.863 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281104, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (365.40, 162.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675410 reads in 159.057 CPU sec, 5.020 real sec
[M::process] read 675282 sequences (100000113 bp) in 2.378 CPU sec, 2.388 real sec...
[M::mem_process_seqs] Processed 675114 reads in 105.089 CPU sec, 3.293 real sec
sorting block 116, #reads = 1076919 mem = 536878473 peak = 1107939328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281340, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (363.66, 161.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675424 sequences (100000153 bp) in 2.629 CPU sec, 2.700 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281157, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (363.45, 160.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675282 reads in 161.210 CPU sec, 5.133 real sec
[M::mem_process_seqs] Processed 675282 reads in 108.592 CPU sec, 3.405 real sec
[M::process] read 675338 sequences (100000154 bp) in 2.192 CPU sec, 2.236 real sec...
sorting block 117, #reads = 1077678 mem = 536877901 peak = 1107939328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281706, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (363.19, 161.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674590 sequences (100000258 bp) in 2.660 CPU sec, 2.706 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280909, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (362.81, 160.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675356 sequences (100000241 bp) in 2.160 CPU sec, 2.159 real sec...
[M::mem_process_seqs] Processed 675424 reads in 167.189 CPU sec, 5.265 real sec
sorting block 118, #reads = 1076551 mem = 536879702 peak = 1107939328
[M::mem_process_seqs] Processed 675338 reads in 111.103 CPU sec, 3.493 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280864, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 353, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (367.18, 159.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675174 sequences (100000283 bp) in 2.619 CPU sec, 2.683 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281494, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 346, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (361.02, 158.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674590 reads in 166.056 CPU sec, 5.237 real sec
[M::process] read 674916 sequences (100000119 bp) in 2.209 CPU sec, 2.212 real sec...
sorting block 119, #reads = 1077089 mem = 536878004 peak = 1107939328
[M::mem_process_seqs] Processed 675356 reads in 107.616 CPU sec, 3.384 real sec
sorting block 120, #reads = 1074911 mem = 536884376 peak = 1146425344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281451, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 345, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (360.62, 158.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675066 sequences (100000050 bp) in 2.782 CPU sec, 2.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281166, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 345, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (359.87, 156.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675174 reads in 161.396 CPU sec, 5.077 real sec
[M::process] read 675184 sequences (100000253 bp) in 2.260 CPU sec, 2.258 real sec...
[M::mem_process_seqs] Processed 674916 reads in 106.184 CPU sec, 3.343 real sec
sorting block 121, #reads = 1076563 mem = 536882133 peak = 1146425344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281442, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 343, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (357.76, 155.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675304 sequences (100000099 bp) in 2.177 CPU sec, 2.254 real sec...
[M::process] read 674836 sequences (100000192 bp) in 1.650 CPU sec, 1.654 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281873, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 343, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (358.37, 156.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675066 reads in 159.794 CPU sec, 5.070 real sec
[M::mem_process_seqs] Processed 675184 reads in 104.540 CPU sec, 3.315 real sec
sorting block 122, #reads = 1077178 mem = 536878866 peak = 1146425344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281202, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 345, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (359.81, 157.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674934 sequences (100000141 bp) in 2.518 CPU sec, 2.569 real sec...
[M::process] read 674988 sequences (100000134 bp) in 1.670 CPU sec, 1.678 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281370, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (363.10, 159.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675304 reads in 160.409 CPU sec, 5.078 real sec
sorting block 123, #reads = 1076684 mem = 536878221 peak = 1146425344
[M::mem_process_seqs] Processed 674836 reads in 103.400 CPU sec, 3.270 real sec
sorting block 124, #reads = 1076402 mem = 536879309 peak = 1146425344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281456, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 348, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (363.83, 160.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676742 sequences (100000212 bp) in 2.671 CPU sec, 2.732 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281229, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (366.80, 163.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674934 reads in 159.099 CPU sec, 5.015 real sec
[M::process] read 681986 sequences (100000094 bp) in 2.427 CPU sec, 2.432 real sec...
[M::mem_process_seqs] Processed 674988 reads in 104.536 CPU sec, 3.282 real sec
sorting block 125, #reads = 1076075 mem = 536880352 peak = 1146425344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281447, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (358.90, 164.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680572 sequences (100000139 bp) in 2.956 CPU sec, 3.007 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283870, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 303, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (325.24, 154.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680022 sequences (100000173 bp) in 2.340 CPU sec, 2.343 real sec...
[M::mem_process_seqs] Processed 676742 reads in 184.672 CPU sec, 5.827 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283332, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (332.66, 155.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1107)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679154 sequences (100000108 bp) in 2.445 CPU sec, 2.452 real sec...
[M::mem_process_seqs] Processed 680572 reads in 104.971 CPU sec, 3.314 real sec
sorting block 126, #reads = 1075161 mem = 536879524 peak = 1146425344
[M::mem_process_seqs] Processed 681986 reads in 256.869 CPU sec, 8.122 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283276, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 317, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (335.99, 155.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 127, #reads = 1075966 mem = 536878339 peak = 1147351040
[M::process] read 678746 sequences (100000133 bp) in 3.298 CPU sec, 3.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282834, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 321, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (338.56, 154.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680022 reads in 205.310 CPU sec, 6.499 real sec
[M::mem_process_seqs] Processed 679154 reads in 123.652 CPU sec, 3.881 real sec
[M::process] read 678814 sequences (100000256 bp) in 2.560 CPU sec, 2.592 real sec...
sorting block 128, #reads = 1076526 mem = 536877856 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283030, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 324, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (342.02, 156.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678722 sequences (100000036 bp) in 2.340 CPU sec, 2.439 real sec...
[M::process] read 679116 sequences (100000218 bp) in 1.720 CPU sec, 1.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282945, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 325, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (343.59, 157.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678746 reads in 182.014 CPU sec, 5.774 real sec
[M::mem_process_seqs] Processed 678814 reads in 119.446 CPU sec, 3.794 real sec
sorting block 129, #reads = 1076901 mem = 536879272 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282528, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 324, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (341.75, 156.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679056 sequences (100000028 bp) in 3.005 CPU sec, 3.064 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282572, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (339.16, 154.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678328 sequences (100000045 bp) in 2.350 CPU sec, 2.348 real sec...
[M::mem_process_seqs] Processed 678722 reads in 189.659 CPU sec, 5.986 real sec
sorting block 130, #reads = 1076890 mem = 536879605 peak = 1147351040
[M::mem_process_seqs] Processed 679116 reads in 127.973 CPU sec, 4.040 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283523, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 325, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (342.54, 157.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677552 sequences (100000270 bp) in 2.932 CPU sec, 2.995 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282801, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (348.01, 159.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677494 sequences (100000276 bp) in 2.330 CPU sec, 2.329 real sec...
[M::mem_process_seqs] Processed 679056 reads in 189.032 CPU sec, 5.964 real sec
sorting block 131, #reads = 1075002 mem = 536881283 peak = 1147351040
[M::mem_process_seqs] Processed 678328 reads in 122.501 CPU sec, 3.867 real sec
sorting block 132, #reads = 1074751 mem = 536879766 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282429, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (349.55, 157.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677358 sequences (100000193 bp) in 2.973 CPU sec, 3.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281584, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 336, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (351.48, 158.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677138 sequences (100000056 bp) in 2.110 CPU sec, 2.112 real sec...
[M::mem_process_seqs] Processed 677552 reads in 179.566 CPU sec, 5.667 real sec
[M::mem_process_seqs] Processed 677494 reads in 117.894 CPU sec, 3.723 real sec
sorting block 133, #reads = 1077219 mem = 536878503 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282268, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 336, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (351.94, 159.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677276 sequences (100000110 bp) in 2.414 CPU sec, 2.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282553, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (354.39, 159.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676596 sequences (100000181 bp) in 2.430 CPU sec, 2.424 real sec...
[M::mem_process_seqs] Processed 677358 reads in 183.009 CPU sec, 5.790 real sec
[M::mem_process_seqs] Processed 677138 reads in 122.083 CPU sec, 3.858 real sec
sorting block 134, #reads = 1077054 mem = 536877978 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282102, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 339, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (355.13, 160.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676068 sequences (100000301 bp) in 2.953 CPU sec, 3.032 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282417, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 345, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (360.83, 163.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676248 sequences (100000301 bp) in 2.430 CPU sec, 2.427 real sec...
[M::mem_process_seqs] Processed 677276 reads in 185.655 CPU sec, 5.862 real sec
sorting block 135, #reads = 1076886 mem = 536878964 peak = 1147351040
[M::mem_process_seqs] Processed 676596 reads in 123.915 CPU sec, 3.903 real sec
sorting block 136, #reads = 1076253 mem = 536878127 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281485, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 346, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (360.95, 161.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675658 sequences (100000026 bp) in 2.731 CPU sec, 2.788 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282118, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 347, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (362.34, 162.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675798 sequences (100000002 bp) in 2.160 CPU sec, 2.154 real sec...
[M::mem_process_seqs] Processed 676068 reads in 183.242 CPU sec, 5.788 real sec
[M::mem_process_seqs] Processed 676248 reads in 121.204 CPU sec, 3.839 real sec
sorting block 137, #reads = 1075569 mem = 536879423 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 281847, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 348, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (363.85, 162.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675722 sequences (100000038 bp) in 3.687 CPU sec, 3.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282496, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 349, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (364.89, 163.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675658 reads in 189.585 CPU sec, 5.999 real sec
[M::mem_process_seqs] Processed 675798 reads in 128.643 CPU sec, 4.044 real sec
[M::process] read 675904 sequences (100000221 bp) in 3.329 CPU sec, 3.385 real sec...
sorting block 138, #reads = 1075226 mem = 536880291 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281274, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 347, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (363.51, 162.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675544 sequences (100000275 bp) in 2.715 CPU sec, 2.784 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281992, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 347, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (363.40, 163.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675722 reads in 187.646 CPU sec, 5.970 real sec
[M::mem_process_seqs] Processed 675904 reads in 124.648 CPU sec, 3.912 real sec
[M::process] read 675308 sequences (100000255 bp) in 2.556 CPU sec, 2.575 real sec...
sorting block 139, #reads = 1075735 mem = 536879915 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281954, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (364.92, 163.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675460 sequences (100000031 bp) in 2.404 CPU sec, 2.489 real sec...
[M::process] read 675464 sequences (100000196 bp) in 1.870 CPU sec, 1.875 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281722, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 349, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (365.82, 164.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675544 reads in 184.038 CPU sec, 5.846 real sec
sorting block 140, #reads = 1075926 mem = 536878786 peak = 1147351040
[M::mem_process_seqs] Processed 675308 reads in 123.045 CPU sec, 3.900 real sec
sorting block 141, #reads = 1077008 mem = 536880342 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281737, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (364.71, 163.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675206 sequences (100000257 bp) in 2.781 CPU sec, 2.854 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281622, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 349, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (364.77, 162.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675324 sequences (100000136 bp) in 2.270 CPU sec, 2.274 real sec...
[M::mem_process_seqs] Processed 675460 reads in 178.946 CPU sec, 5.650 real sec
[M::mem_process_seqs] Processed 675464 reads in 119.574 CPU sec, 3.766 real sec
sorting block 142, #reads = 1076654 mem = 536880133 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281658, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 351, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (367.61, 164.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675136 sequences (100000177 bp) in 3.157 CPU sec, 3.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281458, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 352, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (369.02, 166.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675206 reads in 179.999 CPU sec, 5.683 real sec
[M::process] read 675102 sequences (100000176 bp) in 2.259 CPU sec, 2.259 real sec...
[M::mem_process_seqs] Processed 675324 reads in 120.932 CPU sec, 3.814 real sec
sorting block 143, #reads = 1077185 mem = 536879324 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281163, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (402, 402, 402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (402, 402)
[M::mem_pestat] mean and std.dev: (402.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (402, 402)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 351, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (369.25, 167.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674764 sequences (100000172 bp) in 2.793 CPU sec, 2.888 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281262, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 353, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (370.91, 167.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674760 sequences (100000189 bp) in 2.290 CPU sec, 2.288 real sec...
[M::mem_process_seqs] Processed 675136 reads in 177.844 CPU sec, 5.613 real sec
sorting block 144, #reads = 1077073 mem = 536879613 peak = 1147351040
[M::mem_process_seqs] Processed 675102 reads in 118.148 CPU sec, 3.724 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281807, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 357, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (374.98, 170.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674712 sequences (100000260 bp) in 3.023 CPU sec, 3.108 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281272, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 358, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (375.85, 170.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674764 reads in 175.659 CPU sec, 5.543 real sec
[M::process] read 674900 sequences (100000014 bp) in 2.397 CPU sec, 2.399 real sec...
sorting block 145, #reads = 1077250 mem = 536878182 peak = 1147351040
[M::mem_process_seqs] Processed 674760 reads in 119.054 CPU sec, 3.747 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281459, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 359, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (377.46, 172.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 146, #reads = 1077082 mem = 536878444 peak = 1147351040
[M::process] read 675222 sequences (100000167 bp) in 3.442 CPU sec, 3.727 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281308, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 358, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (377.46, 173.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674712 reads in 183.657 CPU sec, 5.880 real sec
[M::mem_process_seqs] Processed 674900 reads in 125.263 CPU sec, 3.954 real sec
[M::process] read 679642 sequences (100000164 bp) in 3.014 CPU sec, 3.105 real sec...
sorting block 147, #reads = 1075656 mem = 536878294 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281655, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 355, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (374.48, 172.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679496 sequences (100000292 bp) in 2.538 CPU sec, 2.586 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282544, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 318, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (339.90, 159.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679040 sequences (100000038 bp) in 2.220 CPU sec, 2.220 real sec...
[M::mem_process_seqs] Processed 675222 reads in 204.867 CPU sec, 6.477 real sec
[M::mem_process_seqs] Processed 679642 reads in 137.048 CPU sec, 4.319 real sec
sorting block 148, #reads = 1070676 mem = 536879039 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282352, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 320, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (339.25, 157.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678476 sequences (100000222 bp) in 3.346 CPU sec, 3.572 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 282325, 3, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (227, 626, 626)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1424)
[M::mem_pestat] mean and std.dev: (453.64, 213.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1823)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 323, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (341.55, 157.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 679496 reads in 176.435 CPU sec, 5.576 real sec
[M::mem_process_seqs] Processed 679040 reads in 119.579 CPU sec, 3.756 real sec
[M::process] read 678108 sequences (100000205 bp) in 2.677 CPU sec, 2.708 real sec...
sorting block 149, #reads = 1073931 mem = 536879841 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282616, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 326, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (343.05, 155.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678032 sequences (100000049 bp) in 2.938 CPU sec, 3.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281667, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (345.02, 157.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678476 reads in 179.897 CPU sec, 5.687 real sec
[M::process] read 678132 sequences (100000060 bp) in 2.372 CPU sec, 2.379 real sec...
sorting block 150, #reads = 1079992 mem = 536878878 peak = 1147351040
[M::mem_process_seqs] Processed 678108 reads in 119.989 CPU sec, 3.773 real sec
sorting block 151, #reads = 1079591 mem = 536879767 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282260, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (347.17, 158.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677664 sequences (100000102 bp) in 2.301 CPU sec, 2.399 real sec...
[M::process] read 677634 sequences (100000162 bp) in 1.738 CPU sec, 1.745 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282929, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (346.32, 157.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678032 reads in 179.082 CPU sec, 5.699 real sec
[M::mem_process_seqs] Processed 678132 reads in 119.261 CPU sec, 3.785 real sec
sorting block 152, #reads = 1079433 mem = 536883524 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 282177, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (348.06, 156.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677040 sequences (100000163 bp) in 2.669 CPU sec, 2.753 real sec...
[M::process] read 676880 sequences (100000149 bp) in 1.850 CPU sec, 1.846 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282164, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (349.04, 157.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677664 reads in 179.368 CPU sec, 5.674 real sec
sorting block 153, #reads = 1078590 mem = 536878778 peak = 1147351040
[M::mem_process_seqs] Processed 677634 reads in 119.653 CPU sec, 3.801 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281626, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 338, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (353.95, 159.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676960 sequences (100000004 bp) in 2.388 CPU sec, 2.471 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281390, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 339, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (354.52, 159.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676936 sequences (100000170 bp) in 2.365 CPU sec, 2.371 real sec...
[M::mem_process_seqs] Processed 677040 reads in 176.952 CPU sec, 5.586 real sec
sorting block 154, #reads = 1078105 mem = 536879259 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282087, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (356.07, 160.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676736 sequences (100000027 bp) in 2.639 CPU sec, 2.648 real sec...
[M::mem_process_seqs] Processed 676960 reads in 100.407 CPU sec, 3.161 real sec
[M::mem_process_seqs] Processed 676880 reads in 249.649 CPU sec, 7.902 real sec
sorting block 155, #reads = 1079641 mem = 536881372 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282059, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 341, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (357.10, 161.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676910 sequences (100000122 bp) in 3.284 CPU sec, 3.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282269, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 341, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (356.34, 160.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676936 reads in 208.899 CPU sec, 6.612 real sec
[M::mem_process_seqs] Processed 676736 reads in 123.520 CPU sec, 3.884 real sec
[M::process] read 676832 sequences (100000084 bp) in 2.566 CPU sec, 2.618 real sec...
sorting block 156, #reads = 1077705 mem = 536880165 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281808, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 340, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (355.05, 158.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676602 sequences (100000138 bp) in 2.457 CPU sec, 2.556 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281470, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (355.96, 159.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676356 sequences (100000275 bp) in 2.230 CPU sec, 2.234 real sec...
[M::mem_process_seqs] Processed 676910 reads in 182.867 CPU sec, 5.791 real sec
sorting block 157, #reads = 1077468 mem = 536879481 peak = 1147351040
[M::mem_process_seqs] Processed 676832 reads in 120.977 CPU sec, 3.818 real sec
sorting block 158, #reads = 1076558 mem = 536878577 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281585, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 343, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (358.41, 161.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676282 sequences (100000202 bp) in 2.852 CPU sec, 2.927 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281570, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 345, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (359.97, 161.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675994 sequences (100000134 bp) in 2.320 CPU sec, 2.317 real sec...
[M::mem_process_seqs] Processed 676602 reads in 177.798 CPU sec, 5.609 real sec
[M::mem_process_seqs] Processed 676356 reads in 117.986 CPU sec, 3.717 real sec
sorting block 159, #reads = 1077366 mem = 536880293 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281425, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 348, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (361.47, 161.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675336 sequences (100000064 bp) in 2.947 CPU sec, 3.012 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281466, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 346, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (360.68, 159.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675338 sequences (100000192 bp) in 2.320 CPU sec, 2.322 real sec...
[M::mem_process_seqs] Processed 676282 reads in 186.239 CPU sec, 5.883 real sec
[M::mem_process_seqs] Processed 675994 reads in 127.790 CPU sec, 4.029 real sec
sorting block 160, #reads = 1077145 mem = 536880174 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280965, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 352, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (365.67, 160.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675368 sequences (100000207 bp) in 2.294 CPU sec, 2.400 real sec...
[M::process] read 675326 sequences (100000196 bp) in 2.020 CPU sec, 2.023 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281719, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 350, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (363.84, 160.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675336 reads in 181.237 CPU sec, 5.749 real sec
sorting block 161, #reads = 1076271 mem = 536878299 peak = 1147351040
[M::mem_process_seqs] Processed 675338 reads in 120.713 CPU sec, 3.827 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281146, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 349, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (363.87, 160.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675354 sequences (100000221 bp) in 3.089 CPU sec, 3.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 281110, 0, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (2139, 2618, 2618)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1181, 3576)
[M::mem_pestat] mean and std.dev: (2458.33, 225.80)
[M::mem_pestat] low and high boundaries for proper pairs: (702, 4055)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 348, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (363.79, 161.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675526 sequences (100000228 bp) in 2.390 CPU sec, 2.385 real sec...
[M::mem_process_seqs] Processed 675368 reads in 188.936 CPU sec, 5.958 real sec
sorting block 162, #reads = 1076511 mem = 536882486 peak = 1147351040
[M::mem_process_seqs] Processed 675326 reads in 129.946 CPU sec, 4.103 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281203, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (364.01, 161.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 163, #reads = 1075221 mem = 536880356 peak = 1147351040
[M::process] read 675300 sequences (100000290 bp) in 3.383 CPU sec, 3.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281640, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (361.94, 160.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675354 reads in 197.261 CPU sec, 6.284 real sec
[M::process] read 675570 sequences (100000229 bp) in 2.436 CPU sec, 2.461 real sec...
[M::mem_process_seqs] Processed 675526 reads in 133.108 CPU sec, 4.201 real sec
sorting block 164, #reads = 1076423 mem = 536878854 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281655, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 347, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (362.20, 160.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675524 sequences (100000255 bp) in 3.195 CPU sec, 3.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281246, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 347, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (362.92, 162.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675300 reads in 202.082 CPU sec, 6.426 real sec
[M::process] read 675554 sequences (100000005 bp) in 2.425 CPU sec, 2.441 real sec...
[M::mem_process_seqs] Processed 675570 reads in 136.013 CPU sec, 4.301 real sec
sorting block 165, #reads = 1076071 mem = 536878966 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281345, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 346, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (362.67, 162.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675266 sequences (100000021 bp) in 3.149 CPU sec, 3.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281658, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 345, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (361.04, 159.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675278 sequences (100000059 bp) in 2.447 CPU sec, 2.452 real sec...
[M::mem_process_seqs] Processed 675524 reads in 210.752 CPU sec, 6.697 real sec
sorting block 166, #reads = 1074828 mem = 536877433 peak = 1147351040
[M::mem_process_seqs] Processed 675554 reads in 146.206 CPU sec, 4.633 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281550, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 347, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (362.65, 161.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675234 sequences (100000293 bp) in 3.241 CPU sec, 3.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281552, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 349, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (363.89, 161.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675364 sequences (100000227 bp) in 2.423 CPU sec, 2.429 real sec...
[M::mem_process_seqs] Processed 675266 reads in 198.905 CPU sec, 6.302 real sec
sorting block 167, #reads = 1072472 mem = 536879695 peak = 1147351040
[M::mem_process_seqs] Processed 675278 reads in 134.741 CPU sec, 4.255 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280876, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (364.30, 162.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 168, #reads = 1075196 mem = 536881073 peak = 1147351040
[M::process] read 675398 sequences (100000169 bp) in 3.130 CPU sec, 3.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282002, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 349, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (364.77, 163.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675234 reads in 199.936 CPU sec, 6.360 real sec
[M::process] read 675350 sequences (100000160 bp) in 2.480 CPU sec, 2.488 real sec...
[M::mem_process_seqs] Processed 675364 reads in 135.783 CPU sec, 4.284 real sec
sorting block 169, #reads = 1073647 mem = 536879799 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281978, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 349, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (365.31, 163.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675898 sequences (100000273 bp) in 2.814 CPU sec, 2.930 real sec...
[M::process] read 682456 sequences (100000269 bp) in 2.030 CPU sec, 2.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281156, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 350, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (366.70, 165.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 674, 1410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3894)
[M::mem_pestat] mean and std.dev: (901.91, 880.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5136)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675398 reads in 197.623 CPU sec, 6.281 real sec
[M::mem_process_seqs] Processed 675350 reads in 128.660 CPU sec, 4.071 real sec
sorting block 170, #reads = 1073548 mem = 536878923 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281097, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 344, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (363.26, 165.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681584 sequences (100000083 bp) in 2.733 CPU sec, 2.784 real sec...
[M::process] read 680764 sequences (100000079 bp) in 2.390 CPU sec, 2.389 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283815, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 298, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855)
[M::mem_pestat] mean and std.dev: (319.77, 150.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1073)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675898 reads in 204.617 CPU sec, 6.471 real sec
sorting block 171, #reads = 1069717 mem = 536879723 peak = 1147351040
[M::mem_process_seqs] Processed 682456 reads in 133.374 CPU sec, 4.232 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284023, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 304, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865)
[M::mem_pestat] mean and std.dev: (324.30, 151.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1085)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 172, #reads = 1068397 mem = 536879612 peak = 1147351040
[M::process] read 680218 sequences (100000056 bp) in 3.107 CPU sec, 3.241 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283162, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 310, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (328.96, 151.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679730 sequences (100000292 bp) in 2.463 CPU sec, 2.465 real sec...
[M::mem_process_seqs] Processed 681584 reads in 203.564 CPU sec, 6.463 real sec
[M::mem_process_seqs] Processed 680764 reads in 135.338 CPU sec, 4.287 real sec
sorting block 173, #reads = 1076093 mem = 536880317 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283692, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 316, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (333.19, 152.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679344 sequences (100000201 bp) in 2.896 CPU sec, 3.044 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283081, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 318, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (335.20, 152.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680190 sequences (100000057 bp) in 2.423 CPU sec, 2.428 real sec...
[M::mem_process_seqs] Processed 680218 reads in 203.320 CPU sec, 6.468 real sec
[M::mem_process_seqs] Processed 679730 reads in 135.256 CPU sec, 4.278 real sec
sorting block 174, #reads = 1075877 mem = 536879582 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283603, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 321, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (338.26, 154.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1125)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681112 sequences (100000062 bp) in 3.000 CPU sec, 3.240 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283383, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881)
[M::mem_pestat] mean and std.dev: (332.51, 153.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1104)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679926 sequences (100000146 bp) in 2.471 CPU sec, 2.480 real sec...
[M::mem_process_seqs] Processed 679344 reads in 200.841 CPU sec, 6.390 real sec
[M::mem_process_seqs] Processed 680190 reads in 132.945 CPU sec, 4.196 real sec
sorting block 175, #reads = 1077669 mem = 536880278 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282896, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 308, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (326.27, 149.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678962 sequences (100000030 bp) in 2.773 CPU sec, 2.814 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283342, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 679966 sequences (100000119 bp) in 2.370 CPU sec, 2.377 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (214, 317, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (335.35, 153.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (341, 341, 1429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3605)
[M::mem_pestat] mean and std.dev: (925.92, 1158.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5560)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681112 reads in 200.887 CPU sec, 6.353 real sec
sorting block 176, #reads = 1074506 mem = 536878224 peak = 1147351040
[M::mem_process_seqs] Processed 679926 reads in 130.349 CPU sec, 4.137 real sec
sorting block 177, #reads = 1072911 mem = 536878193 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282555, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 325, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (342.36, 156.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678054 sequences (100000025 bp) in 3.089 CPU sec, 3.150 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282964, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 313, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (333.89, 155.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678020 sequences (100000050 bp) in 2.329 CPU sec, 2.333 real sec...
[M::mem_process_seqs] Processed 678962 reads in 188.084 CPU sec, 5.934 real sec
[M::mem_process_seqs] Processed 679966 reads in 122.944 CPU sec, 3.879 real sec
sorting block 178, #reads = 1075388 mem = 536877684 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282199, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (348.39, 158.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677976 sequences (100000287 bp) in 2.840 CPU sec, 2.917 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282588, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (347.85, 157.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677350 sequences (100000218 bp) in 2.360 CPU sec, 2.356 real sec...
[M::mem_process_seqs] Processed 678054 reads in 194.859 CPU sec, 6.159 real sec
[M::mem_process_seqs] Processed 678020 reads in 127.511 CPU sec, 4.034 real sec
sorting block 179, #reads = 1075073 mem = 536877837 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282323, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (348.05, 157.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676690 sequences (100000257 bp) in 3.079 CPU sec, 3.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282006, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (354.57, 159.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676514 sequences (100000287 bp) in 2.480 CPU sec, 2.477 real sec...
[M::mem_process_seqs] Processed 677976 reads in 193.300 CPU sec, 6.104 real sec
sorting block 180, #reads = 1074048 mem = 536881358 peak = 1147351040
[M::mem_process_seqs] Processed 677350 reads in 128.636 CPU sec, 4.055 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281730, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 343, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (358.38, 160.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676502 sequences (100000232 bp) in 2.567 CPU sec, 2.611 real sec...
[M::process] read 676150 sequences (100000055 bp) in 2.380 CPU sec, 2.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281347, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 342, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (357.51, 159.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676690 reads in 186.907 CPU sec, 5.898 real sec
sorting block 181, #reads = 1073635 mem = 536879562 peak = 1147351040
[M::mem_process_seqs] Processed 676514 reads in 125.189 CPU sec, 3.962 real sec
sorting block 182, #reads = 1074055 mem = 536881210 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281527, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 342, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (357.19, 159.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675860 sequences (100000229 bp) in 3.050 CPU sec, 3.092 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281284, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 344, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (358.32, 158.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675778 sequences (100000009 bp) in 2.380 CPU sec, 2.384 real sec...
[M::mem_process_seqs] Processed 676502 reads in 187.139 CPU sec, 5.901 real sec
[M::mem_process_seqs] Processed 676150 reads in 125.783 CPU sec, 3.974 real sec
sorting block 183, #reads = 1073999 mem = 536876499 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281907, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 345, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (359.96, 158.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675848 sequences (100000081 bp) in 2.825 CPU sec, 2.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281983, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 344, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (360.35, 160.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675696 sequences (100000148 bp) in 2.410 CPU sec, 2.409 real sec...
[M::mem_process_seqs] Processed 675860 reads in 185.675 CPU sec, 5.864 real sec
[M::mem_process_seqs] Processed 675778 reads in 124.180 CPU sec, 3.919 real sec
sorting block 184, #reads = 1073684 mem = 536880632 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281356, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 345, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (361.57, 161.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675540 sequences (100000093 bp) in 3.028 CPU sec, 3.084 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281639, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 345, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (362.75, 163.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675460 sequences (100000117 bp) in 2.430 CPU sec, 2.432 real sec...
[M::mem_process_seqs] Processed 675848 reads in 183.849 CPU sec, 5.795 real sec
sorting block 185, #reads = 1075356 mem = 536881398 peak = 1147351040
[M::mem_process_seqs] Processed 675696 reads in 122.037 CPU sec, 3.844 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281926, 11, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 349, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (365.12, 163.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1054, 1054, 2962)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6778)
[M::mem_pestat] mean and std.dev: (1368.45, 1035.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8686)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675346 sequences (100000209 bp) in 2.739 CPU sec, 2.785 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280715, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 349, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (363.98, 161.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674942 sequences (100000041 bp) in 2.350 CPU sec, 2.350 real sec...
[M::mem_process_seqs] Processed 675540 reads in 184.990 CPU sec, 5.846 real sec
sorting block 186, #reads = 1075637 mem = 536880983 peak = 1147351040
[M::mem_process_seqs] Processed 675460 reads in 122.297 CPU sec, 3.864 real sec
sorting block 187, #reads = 1076072 mem = 536877043 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281691, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 674952 sequences (100000028 bp) in 2.504 CPU sec, 2.562 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (238, 346, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (361.27, 159.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674932 sequences (100000131 bp) in 1.770 CPU sec, 1.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281073, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 349, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (363.91, 160.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (432, 432, 557)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (182, 807)
[M::mem_pestat] mean and std.dev: (460.88, 55.84)
[M::mem_pestat] low and high boundaries for proper pairs: (57, 932)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 675346 reads in 179.486 CPU sec, 5.690 real sec
[M::mem_process_seqs] Processed 674942 reads in 118.826 CPU sec, 3.789 real sec
sorting block 188, #reads = 1077458 mem = 536879861 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281468, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 350, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (365.42, 161.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674910 sequences (100000253 bp) in 2.333 CPU sec, 2.401 real sec...
[M::process] read 674826 sequences (100000020 bp) in 1.760 CPU sec, 1.762 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281621, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 350, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (365.14, 160.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674952 reads in 177.434 CPU sec, 5.633 real sec
[M::mem_process_seqs] Processed 674932 reads in 117.324 CPU sec, 3.729 real sec
sorting block 189, #reads = 1077116 mem = 536879620 peak = 1147351040
[M::process] read 674730 sequences (100000046 bp) in 3.076 CPU sec, 3.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281046, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 353, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (368.86, 163.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674580 sequences (100000290 bp) in 2.370 CPU sec, 2.371 real sec...
[M::mem_process_seqs] Processed 674826 reads in 98.105 CPU sec, 3.071 real sec
sorting block 190, #reads = 1076477 mem = 536884555 peak = 1147351040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281048, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 351, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (366.83, 161.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280545, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 354, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (370.14, 164.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674910 reads in 274.469 CPU sec, 8.653 real sec
[M::process] read 674804 sequences (100000054 bp) in 2.582 CPU sec, 2.595 real sec...
[M::mem_process_seqs] Processed 674730 reads in 136.973 CPU sec, 4.327 real sec
sorting block 191, #reads = 1077191 mem = 536890264 peak = 1147351040
[M::process] read 163504 sequences (24172379 bp) in 0.929 CPU sec, 0.949 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281359, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 355, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (371.31, 165.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281251, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 354, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (370.11, 165.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 68003, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 336, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (351.29, 156.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674580 reads in 190.379 CPU sec, 6.152 real sec
sorting block 192, #reads = 1076147 mem = 536879498 peak = 1147351040
[M::mem_process_seqs] Processed 674804 reads in 134.226 CPU sec, 4.330 real sec
[M::mem_process_seqs] Processed 163504 reads in 139.848 CPU sec, 4.523 real sec
sorting block 193, #reads = 1074696 mem = 536877868 peak = 1200050176
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 208355424
overall: 69792624640 mem 30090.537 user 586.663 sys 1011.551 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_bR4-T1-TDNA-1_A23MHWWLT4_1.L002\tPU:L002\tSM:659_bR4-T1-TDNA-1_A23MHWWLT4_1\tLB:659_bR4-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_bR4-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_bR4-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1011.594 sec; CPU: 30677.210 sec
sorting block 194, #reads = 33144 mem = 16715922 peak = 1200050176
uniq header count 1
merging 195 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 11119470568
output reads: 209220240
bam_mem_sort: 195 calls 125.882 user 1.599 sys 128.481 real
bam_write: 195 calls 80.330 user 4.427 sys 107.353 real
execute: 1 calls 67.465 user 2.305 sys 1099.766 real
merge_files: 1 calls 67.429 user 2.282 sys 88.126 real
parse_chunk: 3105 calls 186.664 user 40.250 sys 237.762 real
read_chunk: 8363 calls 83.856 user 53.910 sys 958.018 real
sort_block: 1 calls 60.924 user 53.530 sys 923.206 real
write_chunk: 5256 calls 67.124 user 1.905 sys 87.116 real
overall: 3304558592 mem 3506.982 user 126.521 sys 1099.786 real
ls: cannot access '*.cram': No such file or directory