Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/db/c3d64ea2775ccbb866399ac40e548d/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/72/e2069dad49a8037a46f44d6378c14d/1136_85O-T1-TDNA-1_A23MHWWLT4_1/1136_85O-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/db/c3d64ea2775ccbb866399ac40e548d/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/72/e2069dad49a8037a46f44d6378c14d/1136_85O-T1-TDNA-1_A23MHWWLT4_1/1136_85O-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz ==> STAGING COMPLETE (7 inputs) cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_85O-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1844.690 CPU sec, 69.278 real sec [M::process] read 706200 sequences (100000115 bp) in 1.695 CPU sec, 1.701 real sec... [M::process] read 705252 sequences (100000276 bp) in 1.900 CPU sec, 1.917 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (101, 284978, 40, 90) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 360, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1790) [M::mem_pestat] mean and std.dev: (356.02, 236.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 232, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (258.55, 133.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (359, 930, 2117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5633) [M::mem_pestat] mean and std.dev: (1251.51, 1212.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7391) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 363, 842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2112) [M::mem_pestat] mean and std.dev: (460.94, 378.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2747) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704454 sequences (100000078 bp) in 1.823 CPU sec, 1.853 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (126, 284809, 45, 85) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 297, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152) [M::mem_pestat] mean and std.dev: (303.76, 196.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 234, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (260.88, 135.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (447, 1206, 2151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5559) [M::mem_pestat] mean and std.dev: (1220.19, 1038.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 308, 1592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4406) [M::mem_pestat] mean and std.dev: (728.16, 948.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5813) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703642 sequences (100000072 bp) in 1.781 CPU sec, 1.799 real sec... [M::mem_process_seqs] Processed 706200 reads in 185.712 CPU sec, 5.871 real sec [M::mem_process_seqs] Processed 705252 reads in 128.371 CPU sec, 4.081 real sec sorting block 0, #reads = 1079173 mem = 536883427 peak = 590249984 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 284686, 62, 102) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 289, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1637) [M::mem_pestat] mean and std.dev: (381.14, 330.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 236, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (263.01, 136.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (204, 998, 3127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8973) [M::mem_pestat] mean and std.dev: (1720.26, 1967.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11896) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 335, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1328) [M::mem_pestat] mean and std.dev: (359.93, 253.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1706) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702116 sequences (100000037 bp) in 2.731 CPU sec, 2.939 real sec... [M::process] read 701744 sequences (100000196 bp) in 1.949 CPU sec, 1.958 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (105, 284028, 37, 92) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 274, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175) [M::mem_pestat] mean and std.dev: (321.60, 195.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 238, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (265.61, 137.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (275, 735, 1890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5120) [M::mem_pestat] mean and std.dev: (1126.00, 1056.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6735) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 267, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1385) [M::mem_pestat] mean and std.dev: (346.00, 291.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1790) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704454 reads in 186.108 CPU sec, 5.897 real sec [M::mem_process_seqs] Processed 703642 reads in 210.213 CPU sec, 6.685 real sec sorting block 1, #reads = 1078728 mem = 536880600 peak = 747536384 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 283699, 46, 101) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 330, 907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2369) [M::mem_pestat] mean and std.dev: (471.35, 491.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3100) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 244, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (271.83, 141.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (511, 1237, 2030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5068) [M::mem_pestat] mean and std.dev: (1241.62, 1072.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6587) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 292, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1515) [M::mem_pestat] mean and std.dev: (389.99, 311.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1951) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700820 sequences (100000061 bp) in 2.830 CPU sec, 2.992 real sec... [M::process] read 700952 sequences (100000175 bp) in 1.950 CPU sec, 1.957 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (100, 283967, 43, 91) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 307, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (304.60, 173.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 242, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (269.32, 139.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (326, 908, 3760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10628) [M::mem_pestat] mean and std.dev: (1976.63, 1979.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14062) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 329, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2490) [M::mem_pestat] mean and std.dev: (452.83, 446.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701744 reads in 186.978 CPU sec, 5.922 real sec sorting block 2, #reads = 1077262 mem = 536879597 peak = 796323840 [M::mem_process_seqs] Processed 702116 reads in 210.705 CPU sec, 6.700 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (104, 283375, 45, 109) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 340, 1407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3833) [M::mem_pestat] mean and std.dev: (773.21, 894.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (273.93, 142.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (365, 683, 2971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8183) [M::mem_pestat] mean and std.dev: (1550.95, 1785.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10789) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 293, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1284) [M::mem_pestat] mean and std.dev: (305.51, 190.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700540 sequences (100000181 bp) in 3.429 CPU sec, 3.655 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (115, 283733, 53, 143) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 316, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1504) [M::mem_pestat] mean and std.dev: (399.90, 318.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 246, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (274.52, 143.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (283, 618, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2905) [M::mem_pestat] mean and std.dev: (847.56, 785.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 402, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1483) [M::mem_pestat] mean and std.dev: (415.56, 276.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700526 sequences (100000079 bp) in 2.165 CPU sec, 2.169 real sec... [M::mem_process_seqs] Processed 700820 reads in 189.529 CPU sec, 5.992 real sec sorting block 3, #reads = 1078529 mem = 536879108 peak = 796323840 [M::mem_process_seqs] Processed 700952 reads in 131.799 CPU sec, 4.186 real sec sorting block 4, #reads = 1076486 mem = 536879156 peak = 890232832 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (103, 283868, 42, 136) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 263, 661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1645) [M::mem_pestat] mean and std.dev: (337.52, 282.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 246, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (273.87, 142.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (544, 1202, 2214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5554) [M::mem_pestat] mean and std.dev: (1519.39, 1394.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 351, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2149) [M::mem_pestat] mean and std.dev: (490.22, 441.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2792) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700142 sequences (100000157 bp) in 3.031 CPU sec, 3.251 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (103, 283431, 54, 141) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 257, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (295.79, 192.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (272.70, 140.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (513, 1136, 1942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4800) [M::mem_pestat] mean and std.dev: (983.73, 750.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6229) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 409, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1961) [M::mem_pestat] mean and std.dev: (526.68, 430.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2532) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700434 sequences (100000266 bp) in 2.077 CPU sec, 2.081 real sec... [M::mem_process_seqs] Processed 700540 reads in 196.262 CPU sec, 6.226 real sec [M::mem_process_seqs] Processed 700526 reads in 135.428 CPU sec, 4.287 real sec sorting block 5, #reads = 1076972 mem = 536879114 peak = 890232832 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 283570, 53, 131) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 409, 1770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4854) [M::mem_pestat] mean and std.dev: (1003.84, 1105.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (271.64, 139.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (361, 1207, 3381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9421) [M::mem_pestat] mean and std.dev: (2005.19, 2040.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12441) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 335, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1581) [M::mem_pestat] mean and std.dev: (358.67, 261.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700290 sequences (100000145 bp) in 3.560 CPU sec, 3.713 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 283539, 52, 102) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 273, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1262) [M::mem_pestat] mean and std.dev: (349.14, 247.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 244, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (271.52, 140.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (192, 548, 1740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4836) [M::mem_pestat] mean and std.dev: (769.26, 992.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6384) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 313, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (315.06, 166.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700142 reads in 199.815 CPU sec, 6.329 real sec [M::process] read 699916 sequences (100000158 bp) in 2.827 CPU sec, 2.864 real sec... [M::mem_process_seqs] Processed 700434 reads in 135.441 CPU sec, 4.268 real sec sorting block 6, #reads = 1076887 mem = 536879122 peak = 890232832 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 283274, 39, 111) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 314, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1288) [M::mem_pestat] mean and std.dev: (333.26, 209.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 244, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (271.88, 140.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 1158, 2417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6739) [M::mem_pestat] mean and std.dev: (1443.08, 1382.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8900) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 383, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1436) [M::mem_pestat] mean and std.dev: (408.42, 266.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699702 sequences (100000150 bp) in 2.976 CPU sec, 3.089 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (108, 283281, 50, 144) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 339, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1497) [M::mem_pestat] mean and std.dev: (409.43, 341.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 247, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (273.67, 141.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (706, 1589, 2701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6691) [M::mem_pestat] mean and std.dev: (1935.74, 1610.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8686) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 330, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1613) [M::mem_pestat] mean and std.dev: (382.43, 297.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2086) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699098 sequences (100000160 bp) in 2.390 CPU sec, 2.385 real sec... [M::mem_process_seqs] Processed 700290 reads in 200.019 CPU sec, 6.365 real sec sorting block 7, #reads = 1077307 mem = 536879467 peak = 890232832 [M::mem_process_seqs] Processed 699916 reads in 134.883 CPU sec, 4.276 real sec sorting block 8, #reads = 1077500 mem = 536876917 peak = 890232832 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (101, 283180, 57, 138) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 271, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1198) [M::mem_pestat] mean and std.dev: (295.96, 230.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 247, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (273.60, 140.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (238, 612, 2239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6241) [M::mem_pestat] mean and std.dev: (1226.61, 1415.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 342, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2370) [M::mem_pestat] mean and std.dev: (532.46, 505.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698862 sequences (100000222 bp) in 3.408 CPU sec, 3.568 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (112, 282775, 41, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 414, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2661) [M::mem_pestat] mean and std.dev: (569.90, 565.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 249, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (275.11, 140.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (339, 727, 2496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6810) [M::mem_pestat] mean and std.dev: (1509.68, 1474.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8967) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 291, 624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1476) [M::mem_pestat] mean and std.dev: (322.73, 265.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698406 sequences (100000285 bp) in 2.280 CPU sec, 2.280 real sec... [M::mem_process_seqs] Processed 699702 reads in 196.186 CPU sec, 6.213 real sec [M::mem_process_seqs] Processed 699098 reads in 135.370 CPU sec, 4.275 real sec sorting block 9, #reads = 1076380 mem = 536876260 peak = 890232832 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 283294, 60, 126) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 275, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320) [M::mem_pestat] mean and std.dev: (295.33, 196.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 250, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (275.91, 140.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (788, 1066, 2271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5237) [M::mem_pestat] mean and std.dev: (1399.86, 1059.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6720) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 343, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1346) [M::mem_pestat] mean and std.dev: (377.28, 241.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698480 sequences (100000028 bp) in 2.968 CPU sec, 3.122 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (109, 282721, 40, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 288, 732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1864) [M::mem_pestat] mean and std.dev: (440.73, 408.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 252, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (278.07, 142.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 339, 2221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6151) [M::mem_pestat] mean and std.dev: (1306.08, 1764.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8365) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 285, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1289) [M::mem_pestat] mean and std.dev: (296.40, 167.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697796 sequences (100000059 bp) in 2.280 CPU sec, 2.287 real sec... [M::mem_process_seqs] Processed 698862 reads in 192.905 CPU sec, 6.121 real sec [M::mem_process_seqs] Processed 698406 reads in 132.899 CPU sec, 4.208 real sec sorting block 10, #reads = 1078141 mem = 536879368 peak = 890232832 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 282787, 45, 135) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 282, 840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2158) [M::mem_pestat] mean and std.dev: (364.17, 328.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 252, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (278.47, 143.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (348, 900, 2166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5802) [M::mem_pestat] mean and std.dev: (1480.80, 1530.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7620) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 265, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1450) [M::mem_pestat] mean and std.dev: (369.87, 284.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704714 sequences (100000247 bp) in 3.320 CPU sec, 3.503 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (104, 282501, 41, 98) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 237, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (263.63, 183.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 255, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (281.19, 144.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 998) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (408, 743, 2594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6966) [M::mem_pestat] mean and std.dev: (1431.53, 1397.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9152) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 312, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (325.44, 177.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1431) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698480 reads in 193.549 CPU sec, 6.141 real sec [M::process] read 705524 sequences (100000035 bp) in 2.298 CPU sec, 2.304 real sec... sorting block 11, #reads = 1075933 mem = 536878737 peak = 890232832 [M::mem_process_seqs] Processed 697796 reads in 133.209 CPU sec, 4.225 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 284887, 33, 130) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 239, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1397) [M::mem_pestat] mean and std.dev: (350.85, 304.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 236, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (264.42, 138.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (397, 791, 2412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6442) [M::mem_pestat] mean and std.dev: (1306.16, 1415.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8457) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 318, 692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1674) [M::mem_pestat] mean and std.dev: (323.87, 220.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2165) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 12, #reads = 1075964 mem = 536879049 peak = 890232832 [M::process] read 705176 sequences (100000083 bp) in 3.349 CPU sec, 3.625 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 284946, 23, 102) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 273, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (255.58, 121.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 234, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (262.37, 137.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (782, 1239, 2504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5948) [M::mem_pestat] mean and std.dev: (1719.30, 1434.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7670) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 258, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1254) [M::mem_pestat] mean and std.dev: (295.13, 194.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1613) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704714 reads in 201.871 CPU sec, 6.411 real sec [M::process] read 704212 sequences (100000104 bp) in 2.212 CPU sec, 2.220 real sec... [M::mem_process_seqs] Processed 705524 reads in 139.130 CPU sec, 4.400 real sec sorting block 13, #reads = 1079207 mem = 536881327 peak = 890232832 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (116, 284571, 41, 101) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 410, 1240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3320) [M::mem_pestat] mean and std.dev: (621.61, 699.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 235, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (263.72, 138.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 493, 1730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4826) [M::mem_pestat] mean and std.dev: (807.65, 1038.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6374) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 236, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (248.55, 137.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703590 sequences (100000084 bp) in 3.688 CPU sec, 3.974 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (114, 284724, 43, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 282, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1400) [M::mem_pestat] mean and std.dev: (302.43, 217.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 238, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (266.36, 139.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (135, 371, 1618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4584) [M::mem_pestat] mean and std.dev: (755.10, 945.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6067) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 336, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2570) [M::mem_pestat] mean and std.dev: (457.84, 418.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3357) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 705176 reads in 199.354 CPU sec, 6.340 real sec [M::mem_process_seqs] Processed 704212 reads in 133.146 CPU sec, 4.202 real sec [M::process] read 702506 sequences (100000261 bp) in 2.814 CPU sec, 2.915 real sec... sorting block 14, #reads = 1080741 mem = 536884668 peak = 890232832 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 284557, 41, 99) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 282, 1585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4407) [M::mem_pestat] mean and std.dev: (772.87, 1110.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 239, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (267.97, 140.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 512, 1348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3576) [M::mem_pestat] mean and std.dev: (550.09, 509.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4690) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 350, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1338) [M::mem_pestat] mean and std.dev: (385.54, 242.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1712) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701914 sequences (100000270 bp) in 3.724 CPU sec, 4.171 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 284441, 26, 113) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 393, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3761) [M::mem_pestat] mean and std.dev: (677.51, 778.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 243, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (271.65, 142.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (122, 836, 2073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5975) [M::mem_pestat] mean and std.dev: (1126.54, 1281.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7926) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 352, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1379) [M::mem_pestat] mean and std.dev: (375.72, 228.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1766) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703590 reads in 190.803 CPU sec, 6.103 real sec [M::mem_process_seqs] Processed 702506 reads in 132.160 CPU sec, 4.188 real sec sorting block 15, #reads = 1080519 mem = 536882926 peak = 890232832 [M::process] read 701584 sequences (100000140 bp) in 3.859 CPU sec, 4.183 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (105, 283699, 45, 119) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 262, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1376) [M::mem_pestat] mean and std.dev: (336.27, 257.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 245, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (273.25, 142.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (156, 611, 1462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4074) [M::mem_pestat] mean and std.dev: (748.71, 723.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 279, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1428) [M::mem_pestat] mean and std.dev: (313.57, 228.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1840) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 16, #reads = 1080808 mem = 536879319 peak = 890232832 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (121, 283808, 47, 127) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 476, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2706) [M::mem_pestat] mean and std.dev: (531.05, 454.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 245, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (274.32, 144.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 988) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (570, 1612, 3203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8469) [M::mem_pestat] mean and std.dev: (1882.85, 1636.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11102) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 364, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2482) [M::mem_pestat] mean and std.dev: (535.03, 494.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3231) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701914 reads in 195.047 CPU sec, 6.242 real sec [M::process] read 701016 sequences (100000026 bp) in 3.428 CPU sec, 3.641 real sec... [M::mem_process_seqs] Processed 701584 reads in 133.154 CPU sec, 4.235 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (107, 283726, 53, 122) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 303, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003) [M::mem_pestat] mean and std.dev: (323.31, 183.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1271) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 246, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (274.63, 143.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (474, 1054, 2987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8013) [M::mem_pestat] mean and std.dev: (1778.89, 1792.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (310, 428, 886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2038) [M::mem_pestat] mean and std.dev: (569.41, 474.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2614) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 17, #reads = 1079471 mem = 536887363 peak = 890232832 [M::process] read 701254 sequences (100000263 bp) in 3.983 CPU sec, 4.320 real sec... [M::mem_process_seqs] Processed 701016 reads in 111.272 CPU sec, 3.653 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (137, 284002, 36, 134) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 302, 705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1769) [M::mem_pestat] mean and std.dev: (445.99, 434.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 244, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (273.40, 143.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (512, 982, 3630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9866) [M::mem_pestat] mean and std.dev: (2048.69, 2223.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12984) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 309, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1401) [M::mem_pestat] mean and std.dev: (311.66, 209.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701254 reads in 111.658 CPU sec, 3.660 real sec sorting block 18, #reads = 1077834 mem = 536879119 peak = 890232832 [M::process] read 700926 sequences (100000080 bp) in 3.990 CPU sec, 4.383 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (111, 283677, 59, 99) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 347, 1012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2722) [M::mem_pestat] mean and std.dev: (519.82, 502.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 245, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (273.19, 141.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (338, 1167, 2468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6728) [M::mem_pestat] mean and std.dev: (1251.06, 1386.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8858) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 301, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1679) [M::mem_pestat] mean and std.dev: (385.41, 327.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2185) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701202 sequences (100000131 bp) in 3.217 CPU sec, 3.552 real sec... [M::mem_process_seqs] Processed 700926 reads in 114.797 CPU sec, 3.701 real sec sorting block 19, #reads = 1078030 mem = 536876680 peak = 890232832 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (114, 284092, 45, 114) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 291, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1964) [M::mem_pestat] mean and std.dev: (405.93, 392.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 243, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (269.85, 138.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (243, 593, 1258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3288) [M::mem_pestat] mean and std.dev: (564.92, 517.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4303) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 330, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036) [M::mem_pestat] mean and std.dev: (341.10, 165.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1303) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701202 reads in 112.222 CPU sec, 3.607 real sec [M::process] read 701000 sequences (100000149 bp) in 3.913 CPU sec, 4.226 real sec... sorting block 20, #reads = 1077266 mem = 536878441 peak = 890232832 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 283890, 42, 101) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 247, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2029) [M::mem_pestat] mean and std.dev: (398.58, 359.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 243, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (269.87, 138.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (588, 1316, 2649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6771) [M::mem_pestat] mean and std.dev: (1804.78, 1533.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8832) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 291, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1473) [M::mem_pestat] mean and std.dev: (351.30, 241.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700638 sequences (100000187 bp) in 2.934 CPU sec, 3.115 real sec... [M::mem_process_seqs] Processed 701000 reads in 113.470 CPU sec, 3.649 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (105, 282930, 35, 118) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 306, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1521) [M::mem_pestat] mean and std.dev: (386.04, 315.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (270.42, 139.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (389, 1056, 4213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11861) [M::mem_pestat] mean and std.dev: (2071.83, 2267.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15685) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 330, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1607) [M::mem_pestat] mean and std.dev: (386.24, 285.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2079) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700204 sequences (100000006 bp) in 3.437 CPU sec, 3.695 real sec... [M::mem_process_seqs] Processed 700638 reads in 114.699 CPU sec, 3.678 real sec sorting block 21, #reads = 1077679 mem = 536880170 peak = 890232832 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 283471, 47, 93) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 258, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (270.10, 161.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (271.90, 139.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (287, 1036, 1880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5066) [M::mem_pestat] mean and std.dev: (1164.67, 1009.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 339, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1385) [M::mem_pestat] mean and std.dev: (343.88, 201.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1784) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700234 sequences (100000104 bp) in 3.567 CPU sec, 3.796 real sec... [M::mem_process_seqs] Processed 700204 reads in 112.973 CPU sec, 3.636 real sec sorting block 22, #reads = 1076322 mem = 536884091 peak = 890232832 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (115, 283419, 50, 110) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 296, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1277) [M::mem_pestat] mean and std.dev: (335.77, 251.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (272.68, 139.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (489, 1034, 4322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11988) [M::mem_pestat] mean and std.dev: (2489.42, 2612.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15821) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 295, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1851) [M::mem_pestat] mean and std.dev: (435.62, 376.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2409) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699646 sequences (100000073 bp) in 3.623 CPU sec, 3.868 real sec... [M::mem_process_seqs] Processed 700234 reads in 111.813 CPU sec, 3.589 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 282834, 50, 123) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 256, 656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1602) [M::mem_pestat] mean and std.dev: (353.32, 292.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 248, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (274.81, 141.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (685, 1583, 2948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7474) [M::mem_pestat] mean and std.dev: (1881.25, 1755.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9737) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 260, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1419) [M::mem_pestat] mean and std.dev: (339.53, 252.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699646 reads in 110.427 CPU sec, 3.554 real sec [M::process] read 699132 sequences (100000266 bp) in 3.734 CPU sec, 4.010 real sec... sorting block 23, #reads = 1076444 mem = 536880810 peak = 890232832 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (98, 283069, 43, 90) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 309, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1166) [M::mem_pestat] mean and std.dev: (312.38, 183.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 249, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (275.79, 142.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (169, 492, 2058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5836) [M::mem_pestat] mean and std.dev: (920.98, 1112.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7725) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 290, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1618) [M::mem_pestat] mean and std.dev: (422.23, 339.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699132 reads in 110.564 CPU sec, 3.548 real sec [M::process] read 699418 sequences (100000188 bp) in 3.417 CPU sec, 3.623 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (107, 283373, 45, 120) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 337, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1970) [M::mem_pestat] mean and std.dev: (401.51, 380.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 249, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (276.16, 142.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (169, 395, 2056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5830) [M::mem_pestat] mean and std.dev: (1184.16, 1585.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 323, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (326.67, 183.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699418 reads in 106.524 CPU sec, 3.427 real sec sorting block 24, #reads = 1075385 mem = 536877602 peak = 890232832 [M::process] read 700008 sequences (100000062 bp) in 4.203 CPU sec, 4.558 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (107, 283239, 37, 113) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 234, 722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1806) [M::mem_pestat] mean and std.dev: (302.49, 241.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (275.04, 143.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (520, 1114, 4211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11593) [M::mem_pestat] mean and std.dev: (2529.22, 2898.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15284) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 345, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1744) [M::mem_pestat] mean and std.dev: (416.10, 315.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2260) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703662 sequences (100000023 bp) in 3.157 CPU sec, 3.303 real sec... [M::mem_process_seqs] Processed 700008 reads in 111.005 CPU sec, 3.555 real sec sorting block 25, #reads = 1076109 mem = 536881069 peak = 890232832 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (104, 284548, 56, 98) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 268, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1243) [M::mem_pestat] mean and std.dev: (320.32, 222.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 238, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (266.52, 139.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (146, 737, 1189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3275) [M::mem_pestat] mean and std.dev: (742.12, 731.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4318) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 339, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2978) [M::mem_pestat] mean and std.dev: (528.38, 547.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707446 sequences (100000017 bp) in 3.153 CPU sec, 3.414 real sec... [M::mem_process_seqs] Processed 703662 reads in 113.185 CPU sec, 3.636 real sec [M::process] read 707238 sequences (100000113 bp) in 2.910 CPU sec, 3.129 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (98, 285319, 35, 105) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 303, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (293.52, 164.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 229, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (257.40, 134.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1484, 3311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8707) [M::mem_pestat] mean and std.dev: (2112.50, 2079.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11405) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 291, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (297.02, 166.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1244) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705636 sequences (100000206 bp) in 1.698 CPU sec, 1.700 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (100, 285855, 41, 92) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 270, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (273.03, 169.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 230, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (258.27, 134.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (437, 777, 1475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3551) [M::mem_pestat] mean and std.dev: (869.34, 606.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4589) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 308, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1378) [M::mem_pestat] mean and std.dev: (313.95, 173.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1766) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704420 sequences (100000279 bp) in 1.589 CPU sec, 1.589 real sec... [M::mem_process_seqs] Processed 707446 reads in 195.092 CPU sec, 6.135 real sec sorting block 26, #reads = 1077282 mem = 536879674 peak = 890232832 [M::mem_process_seqs] Processed 707238 reads in 135.880 CPU sec, 4.291 real sec sorting block 27, #reads = 1080923 mem = 536878210 peak = 1019568128 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (98, 285051, 43, 99) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 409, 1100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2848) [M::mem_pestat] mean and std.dev: (552.43, 474.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 235, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (262.16, 136.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (749, 1139, 2188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5066) [M::mem_pestat] mean and std.dev: (1300.40, 1115.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6505) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 326, 681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1657) [M::mem_pestat] mean and std.dev: (396.75, 317.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2145) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703288 sequences (100000160 bp) in 3.774 CPU sec, 4.053 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (109, 284510, 28, 139) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 276, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (310.47, 191.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 237, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (264.74, 137.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (543, 902, 2889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7581) [M::mem_pestat] mean and std.dev: (1882.04, 2075.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10184) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 303, 828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2068) [M::mem_pestat] mean and std.dev: (429.38, 361.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2688) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 705636 reads in 193.740 CPU sec, 6.126 real sec [M::process] read 702480 sequences (100000179 bp) in 2.603 CPU sec, 2.661 real sec... [M::mem_process_seqs] Processed 704420 reads in 133.140 CPU sec, 4.188 real sec sorting block 28, #reads = 1081274 mem = 536879770 peak = 1019568128 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (106, 284752, 52, 110) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 276, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1601) [M::mem_pestat] mean and std.dev: (375.79, 288.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 241, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (268.10, 138.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (356, 756, 2309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6215) [M::mem_pestat] mean and std.dev: (962.94, 997.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8168) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 322, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1264) [M::mem_pestat] mean and std.dev: (337.83, 212.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1616) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701806 sequences (100000008 bp) in 3.220 CPU sec, 3.440 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 283766, 53, 105) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 302, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2069) [M::mem_pestat] mean and std.dev: (427.82, 437.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 242, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (269.04, 138.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (349, 858, 1835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4807) [M::mem_pestat] mean and std.dev: (945.39, 737.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6293) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 353, 945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2349) [M::mem_pestat] mean and std.dev: (572.53, 497.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3051) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701430 sequences (100000002 bp) in 2.099 CPU sec, 2.105 real sec... [M::mem_process_seqs] Processed 703288 reads in 192.793 CPU sec, 6.102 real sec sorting block 29, #reads = 1079035 mem = 536883390 peak = 1019568128 [M::mem_process_seqs] Processed 702480 reads in 134.378 CPU sec, 4.246 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 284048, 32, 104) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 313, 683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1645) [M::mem_pestat] mean and std.dev: (399.73, 309.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2126) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 245, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (271.54, 140.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (170, 467, 1076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2888) [M::mem_pestat] mean and std.dev: (647.67, 709.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3794) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 265, 589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1357) [M::mem_pestat] mean and std.dev: (349.00, 239.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701258 sequences (100000219 bp) in 3.120 CPU sec, 3.412 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (110, 283906, 43, 121) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 234, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033) [M::mem_pestat] mean and std.dev: (283.76, 190.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 245, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (272.31, 140.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (571, 1180, 2813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7297) [M::mem_pestat] mean and std.dev: (1551.44, 1366.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9539) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 412, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158) [M::mem_pestat] mean and std.dev: (374.16, 219.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1470) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701468 sequences (100000172 bp) in 1.813 CPU sec, 1.820 real sec... [M::mem_process_seqs] Processed 701806 reads in 194.791 CPU sec, 6.181 real sec sorting block 30, #reads = 1078574 mem = 536883045 peak = 1019568128 [M::mem_process_seqs] Processed 701430 reads in 134.517 CPU sec, 4.267 real sec sorting block 31, #reads = 1077910 mem = 536881502 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 283452, 31, 91) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 326, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247) [M::mem_pestat] mean and std.dev: (352.73, 231.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 245, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (272.69, 141.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (692, 1891, 2990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7586) [M::mem_pestat] mean and std.dev: (1603.39, 1345.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9884) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 311, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1368) [M::mem_pestat] mean and std.dev: (336.91, 231.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1759) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700630 sequences (100000294 bp) in 3.172 CPU sec, 3.353 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (110, 283768, 33, 118) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 337, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1339) [M::mem_pestat] mean and std.dev: (382.69, 262.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 244, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (270.31, 139.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (169, 526, 1957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5533) [M::mem_pestat] mean and std.dev: (1266.18, 1511.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 302, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271) [M::mem_pestat] mean and std.dev: (339.57, 216.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1637) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700720 sequences (100000017 bp) in 2.411 CPU sec, 2.419 real sec... [M::mem_process_seqs] Processed 701258 reads in 194.344 CPU sec, 6.136 real sec [M::mem_process_seqs] Processed 701468 reads in 133.655 CPU sec, 4.218 real sec sorting block 32, #reads = 1078416 mem = 536881702 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 283354, 44, 104) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 289, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1839) [M::mem_pestat] mean and std.dev: (380.66, 309.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (272.02, 139.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (419, 1076, 2001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5165) [M::mem_pestat] mean and std.dev: (1190.17, 974.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6747) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 331, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1277) [M::mem_pestat] mean and std.dev: (338.60, 213.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1643) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700450 sequences (100000147 bp) in 3.721 CPU sec, 3.974 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (132, 283189, 54, 107) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 336, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1593) [M::mem_pestat] mean and std.dev: (390.03, 311.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 244, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (270.60, 138.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (157, 646, 1464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4078) [M::mem_pestat] mean and std.dev: (875.83, 873.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5385) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 340, 576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1330) [M::mem_pestat] mean and std.dev: (394.44, 290.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1707) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700630 reads in 195.010 CPU sec, 6.167 real sec [M::mem_process_seqs] Processed 700720 reads in 135.887 CPU sec, 4.277 real sec [M::process] read 699978 sequences (100000227 bp) in 2.993 CPU sec, 3.110 real sec... sorting block 33, #reads = 1077288 mem = 536879262 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (108, 283796, 67, 109) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 452, 940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2414) [M::mem_pestat] mean and std.dev: (538.68, 517.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (271.58, 139.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (210, 734, 1731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4773) [M::mem_pestat] mean and std.dev: (915.60, 978.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6294) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 308, 858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2162) [M::mem_pestat] mean and std.dev: (497.67, 460.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2814) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700266 sequences (100000085 bp) in 3.651 CPU sec, 3.889 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (131, 283145, 42, 114) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 302, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1203) [M::mem_pestat] mean and std.dev: (290.43, 167.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (273.05, 140.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 887, 2066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5588) [M::mem_pestat] mean and std.dev: (1252.58, 1324.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 325, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1853) [M::mem_pestat] mean and std.dev: (436.32, 382.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2404) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700450 reads in 196.790 CPU sec, 6.244 real sec sorting block 34, #reads = 1077442 mem = 536883157 peak = 1021329408 [M::mem_process_seqs] Processed 699978 reads in 136.954 CPU sec, 4.314 real sec [M::process] read 700234 sequences (100000179 bp) in 3.386 CPU sec, 3.560 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (114, 283135, 43, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 298, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1579) [M::mem_pestat] mean and std.dev: (352.98, 315.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (271.12, 138.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (494, 1270, 3513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9551) [M::mem_pestat] mean and std.dev: (1974.21, 1862.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12570) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 394, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3115) [M::mem_pestat] mean and std.dev: (552.73, 551.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4081) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 35, #reads = 1076256 mem = 536879219 peak = 1021329408 [M::process] read 700222 sequences (100000006 bp) in 3.650 CPU sec, 3.916 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (109, 282942, 70, 123) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 345, 1084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2796) [M::mem_pestat] mean and std.dev: (612.01, 586.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (272.97, 140.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (485, 886, 1670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4040) [M::mem_pestat] mean and std.dev: (980.08, 841.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5225) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 271, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (277.38, 134.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700266 reads in 198.692 CPU sec, 6.324 real sec [M::mem_process_seqs] Processed 700234 reads in 139.219 CPU sec, 4.391 real sec [M::process] read 700068 sequences (100000133 bp) in 3.690 CPU sec, 3.961 real sec... sorting block 36, #reads = 1075782 mem = 536879180 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (117, 283765, 54, 94) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 306, 675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1693) [M::mem_pestat] mean and std.dev: (421.34, 387.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 246, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (273.95, 142.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (455, 823, 1536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3698) [M::mem_pestat] mean and std.dev: (1028.38, 772.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4779) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 253, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (278.26, 157.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1239) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700262 sequences (100000157 bp) in 3.159 CPU sec, 3.347 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 283602, 36, 118) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 292, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1725) [M::mem_pestat] mean and std.dev: (388.86, 334.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2243) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (273.70, 141.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (728, 1134, 1870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4154) [M::mem_pestat] mean and std.dev: (1162.44, 877.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5296) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 293, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1456) [M::mem_pestat] mean and std.dev: (335.77, 224.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700222 reads in 201.909 CPU sec, 6.433 real sec [M::mem_process_seqs] Processed 700068 reads in 140.032 CPU sec, 4.414 real sec sorting block 37, #reads = 1075257 mem = 536880187 peak = 1021329408 [M::process] read 704136 sequences (100000262 bp) in 3.006 CPU sec, 3.210 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (103, 283200, 36, 112) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 290, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367) [M::mem_pestat] mean and std.dev: (351.56, 255.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (272.84, 141.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (306, 986, 1357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3459) [M::mem_pestat] mean and std.dev: (763.87, 530.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4510) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 384, 691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1661) [M::mem_pestat] mean and std.dev: (484.21, 423.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2179) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706188 sequences (100000189 bp) in 3.360 CPU sec, 3.629 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (119, 283760, 42, 111) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 262, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (250.44, 124.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 236, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (263.58, 136.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (91, 678, 2049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5965) [M::mem_pestat] mean and std.dev: (1303.71, 1661.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7948) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (258, 354, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (316.64, 123.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700262 reads in 198.975 CPU sec, 6.332 real sec [M::mem_process_seqs] Processed 704136 reads in 141.581 CPU sec, 4.468 real sec sorting block 38, #reads = 1075707 mem = 536875477 peak = 1021329408 [M::process] read 705704 sequences (100000057 bp) in 3.292 CPU sec, 3.506 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (95, 285402, 33, 100) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 445, 1450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3982) [M::mem_pestat] mean and std.dev: (776.02, 805.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5248) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 234, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (261.35, 136.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (88, 627, 2037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5935) [M::mem_pestat] mean and std.dev: (1059.42, 1226.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7884) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 309, 946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2462) [M::mem_pestat] mean and std.dev: (447.36, 419.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3220) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 39, #reads = 1075389 mem = 536878512 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (117, 285430, 40, 102) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 361, 1108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2872) [M::mem_pestat] mean and std.dev: (675.79, 742.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 234, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (262.45, 137.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 1274, 2299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6407) [M::mem_pestat] mean and std.dev: (1277.57, 1361.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8461) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 312, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1260) [M::mem_pestat] mean and std.dev: (309.00, 190.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1615) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704738 sequences (100000223 bp) in 3.621 CPU sec, 3.860 real sec... [M::mem_process_seqs] Processed 706188 reads in 192.910 CPU sec, 6.165 real sec [M::mem_process_seqs] Processed 705704 reads in 137.187 CPU sec, 4.338 real sec sorting block 40, #reads = 1081587 mem = 536879338 peak = 1021329408 [M::process] read 704280 sequences (100000283 bp) in 3.896 CPU sec, 4.225 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (100, 284649, 42, 116) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 334, 1374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3682) [M::mem_pestat] mean and std.dev: (770.74, 872.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 236, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (264.49, 137.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (121, 635, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2851) [M::mem_pestat] mean and std.dev: (604.75, 598.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 388, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2131) [M::mem_pestat] mean and std.dev: (515.41, 437.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 284419, 54, 104) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 250, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1427) [M::mem_pestat] mean and std.dev: (315.36, 247.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 238, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (265.71, 138.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 640, 1500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3980) [M::mem_pestat] mean and std.dev: (698.61, 678.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5220) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 360, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (335.88, 163.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1559) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703198 sequences (100000133 bp) in 3.290 CPU sec, 3.444 real sec... [M::mem_process_seqs] Processed 704738 reads in 195.343 CPU sec, 6.235 real sec [M::mem_process_seqs] Processed 704280 reads in 136.973 CPU sec, 4.341 real sec sorting block 41, #reads = 1080642 mem = 536878595 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 284173, 46, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 390, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2931) [M::mem_pestat] mean and std.dev: (583.98, 536.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 241, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (268.66, 139.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (125, 698, 2594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7532) [M::mem_pestat] mean and std.dev: (1176.90, 1542.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10001) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 298, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009) [M::mem_pestat] mean and std.dev: (277.56, 137.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702326 sequences (100000038 bp) in 3.962 CPU sec, 4.318 real sec... [M::mem_process_seqs] Processed 703198 reads in 114.522 CPU sec, 3.747 real sec sorting block 42, #reads = 1079884 mem = 536881904 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (110, 284348, 67, 129) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 387, 1273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3447) [M::mem_pestat] mean and std.dev: (737.53, 757.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 243, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (271.11, 140.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (348, 801, 1694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4386) [M::mem_pestat] mean and std.dev: (887.64, 879.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 268, 740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1846) [M::mem_pestat] mean and std.dev: (418.13, 361.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702362 sequences (100000043 bp) in 3.681 CPU sec, 4.033 real sec... [M::mem_process_seqs] Processed 702326 reads in 114.524 CPU sec, 3.732 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (97, 284012, 27, 123) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 285, 854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2156) [M::mem_pestat] mean and std.dev: (535.67, 534.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 244, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (271.49, 140.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 534, 1237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3575) [M::mem_pestat] mean and std.dev: (888.59, 965.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4750) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 331, 1112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2888) [M::mem_pestat] mean and std.dev: (481.98, 410.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3776) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 43, #reads = 1080461 mem = 536881589 peak = 1021329408 [M::mem_process_seqs] Processed 702362 reads in 111.987 CPU sec, 3.640 real sec [M::process] read 701662 sequences (100000245 bp) in 4.144 CPU sec, 4.520 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (125, 283591, 48, 117) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 340, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1426) [M::mem_pestat] mean and std.dev: (346.71, 206.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 244, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (272.36, 141.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 789, 3382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9782) [M::mem_pestat] mean and std.dev: (1817.27, 2482.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12982) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 343, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1458) [M::mem_pestat] mean and std.dev: (357.78, 231.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702030 sequences (100000011 bp) in 3.269 CPU sec, 3.443 real sec... [M::mem_process_seqs] Processed 701662 reads in 111.962 CPU sec, 3.599 real sec sorting block 44, #reads = 1078851 mem = 536877846 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (114, 284027, 42, 103) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 413, 2289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6443) [M::mem_pestat] mean and std.dev: (1247.81, 1573.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 244, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (271.66, 140.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (224, 809, 2135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5957) [M::mem_pestat] mean and std.dev: (1212.85, 1106.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7868) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 394, 1250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3334) [M::mem_pestat] mean and std.dev: (584.39, 550.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4376) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702030 reads in 113.066 CPU sec, 3.632 real sec [M::process] read 703158 sequences (100000048 bp) in 3.561 CPU sec, 3.802 real sec... sorting block 45, #reads = 1077938 mem = 536878125 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (107, 284674, 32, 97) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 262, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2402) [M::mem_pestat] mean and std.dev: (500.74, 516.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3143) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 237, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (265.30, 137.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 672, 4585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13333) [M::mem_pestat] mean and std.dev: (1935.34, 2194.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17707) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 362, 568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1318) [M::mem_pestat] mean and std.dev: (337.20, 213.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1693) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703158 reads in 111.494 CPU sec, 3.588 real sec [M::process] read 701566 sequences (100000101 bp) in 3.671 CPU sec, 3.922 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (108, 283964, 50, 104) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 291, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1960) [M::mem_pestat] mean and std.dev: (396.11, 362.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (267.00, 137.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 494, 1667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4533) [M::mem_pestat] mean and std.dev: (676.25, 737.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5966) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 433, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3899) [M::mem_pestat] mean and std.dev: (849.90, 910.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5123) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701748 sequences (100000011 bp) in 3.164 CPU sec, 3.327 real sec... [M::mem_process_seqs] Processed 701566 reads in 108.082 CPU sec, 3.478 real sec sorting block 46, #reads = 1078112 mem = 536879439 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (116, 283292, 56, 112) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 301, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2445) [M::mem_pestat] mean and std.dev: (577.80, 576.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3206) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (267.05, 137.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (538, 1087, 2567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6625) [M::mem_pestat] mean and std.dev: (1680.96, 1808.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8913) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 331, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1666) [M::mem_pestat] mean and std.dev: (366.23, 301.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2162) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700794 sequences (100000244 bp) in 3.599 CPU sec, 3.853 real sec... [M::mem_process_seqs] Processed 701748 reads in 115.520 CPU sec, 3.713 real sec sorting block 47, #reads = 1075996 mem = 536877799 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 283471, 46, 110) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 314, 884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2258) [M::mem_pestat] mean and std.dev: (507.05, 506.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (268.18, 136.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (172, 578, 2433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6955) [M::mem_pestat] mean and std.dev: (1080.40, 1358.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9216) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 302, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1828) [M::mem_pestat] mean and std.dev: (395.73, 350.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700794 reads in 114.102 CPU sec, 3.655 real sec [M::process] read 701032 sequences (100000123 bp) in 3.589 CPU sec, 3.843 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (108, 282749, 47, 126) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 260, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1786) [M::mem_pestat] mean and std.dev: (351.31, 293.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 242, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (267.96, 136.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (579, 911, 3393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9021) [M::mem_pestat] mean and std.dev: (2073.09, 2267.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11835) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 301, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1162) [M::mem_pestat] mean and std.dev: (313.07, 189.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1490) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701032 reads in 109.408 CPU sec, 3.522 real sec [M::process] read 700436 sequences (100000195 bp) in 3.402 CPU sec, 3.595 real sec... sorting block 48, #reads = 1076715 mem = 536887456 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (136, 283647, 32, 129) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 358, 859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2133) [M::mem_pestat] mean and std.dev: (484.62, 448.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (268.39, 135.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (362, 708, 1907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4997) [M::mem_pestat] mean and std.dev: (1058.90, 1019.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6542) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 324, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (293.43, 154.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700436 reads in 112.670 CPU sec, 3.612 real sec [M::process] read 700436 sequences (100000152 bp) in 3.521 CPU sec, 3.739 real sec... sorting block 49, #reads = 1075875 mem = 536879259 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 283551, 28, 124) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 256, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (283.40, 132.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 244, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (269.24, 136.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (446, 669, 1337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3119) [M::mem_pestat] mean and std.dev: (818.27, 631.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4010) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 344, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2384) [M::mem_pestat] mean and std.dev: (471.64, 431.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3108) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700436 reads in 107.564 CPU sec, 3.466 real sec [M::process] read 700024 sequences (100000113 bp) in 3.370 CPU sec, 3.549 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 283504, 49, 108) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 401, 1032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2722) [M::mem_pestat] mean and std.dev: (570.40, 560.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (271.26, 138.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (410, 1457, 1957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5051) [M::mem_pestat] mean and std.dev: (1336.02, 1279.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 273, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158) [M::mem_pestat] mean and std.dev: (300.01, 184.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1476) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700024 reads in 109.318 CPU sec, 3.517 real sec [M::process] read 700296 sequences (100000244 bp) in 3.404 CPU sec, 3.603 real sec... sorting block 50, #reads = 1075806 mem = 536880395 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 283293, 58, 110) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 310, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1732) [M::mem_pestat] mean and std.dev: (395.39, 323.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2247) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (272.24, 140.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (484, 1182, 1992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5008) [M::mem_pestat] mean and std.dev: (1312.45, 1058.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6516) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 417, 1191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3053) [M::mem_pestat] mean and std.dev: (617.61, 577.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700134 sequences (100000183 bp) in 3.266 CPU sec, 3.413 real sec... [M::mem_process_seqs] Processed 700296 reads in 107.693 CPU sec, 3.462 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (118, 283460, 37, 108) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 311, 967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2609) [M::mem_pestat] mean and std.dev: (517.50, 567.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (273.60, 141.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (459, 1019, 2271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5895) [M::mem_pestat] mean and std.dev: (1250.32, 1184.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7707) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 418, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429) [M::mem_pestat] mean and std.dev: (440.78, 308.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700134 reads in 107.093 CPU sec, 3.443 real sec sorting block 51, #reads = 1075296 mem = 536881630 peak = 1021329408 [M::process] read 706440 sequences (100000143 bp) in 3.549 CPU sec, 3.738 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (102, 285383, 36, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 295, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2656) [M::mem_pestat] mean and std.dev: (508.84, 565.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 227, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (255.75, 133.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (134, 389, 1419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3989) [M::mem_pestat] mean and std.dev: (648.62, 864.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 372, 942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2476) [M::mem_pestat] mean and std.dev: (458.21, 487.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3243) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 710430 sequences (100000082 bp) in 3.084 CPU sec, 3.181 real sec... [M::mem_process_seqs] Processed 706440 reads in 114.923 CPU sec, 3.677 real sec sorting block 52, #reads = 1075815 mem = 536879779 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (99, 286612, 33, 117) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 322, 1107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3001) [M::mem_pestat] mean and std.dev: (689.01, 797.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 218, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (245.63, 128.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (263, 1586, 2784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7826) [M::mem_pestat] mean and std.dev: (1847.75, 2026.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10347) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 338, 677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1617) [M::mem_pestat] mean and std.dev: (410.84, 292.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2087) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707064 sequences (100000004 bp) in 3.380 CPU sec, 3.558 real sec... [M::mem_process_seqs] Processed 710430 reads in 112.611 CPU sec, 3.602 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 286116, 41, 117) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 287, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1521) [M::mem_pestat] mean and std.dev: (350.32, 266.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 226, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (255.51, 133.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (148, 674, 1957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5575) [M::mem_pestat] mean and std.dev: (1032.83, 1114.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7384) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 306, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325) [M::mem_pestat] mean and std.dev: (337.04, 246.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1709) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705878 sequences (100000194 bp) in 3.352 CPU sec, 3.539 real sec... [M::mem_process_seqs] Processed 707064 reads in 103.492 CPU sec, 3.321 real sec sorting block 53, #reads = 1079066 mem = 536881553 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (96, 285433, 34, 105) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 250, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1377) [M::mem_pestat] mean and std.dev: (355.08, 280.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 230, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (260.48, 137.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (514, 997, 2210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5602) [M::mem_pestat] mean and std.dev: (1428.77, 1395.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 361, 854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2126) [M::mem_pestat] mean and std.dev: (516.05, 435.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704926 sequences (100000075 bp) in 3.399 CPU sec, 3.578 real sec... [M::mem_process_seqs] Processed 705878 reads in 103.622 CPU sec, 3.318 real sec sorting block 54, #reads = 1079322 mem = 536879476 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (113, 285349, 41, 101) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 319, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1693) [M::mem_pestat] mean and std.dev: (353.67, 270.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 233, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (262.50, 138.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (360, 1048, 1666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4278) [M::mem_pestat] mean and std.dev: (1056.59, 863.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5584) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 301, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2024) [M::mem_pestat] mean and std.dev: (399.12, 331.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2630) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704926 reads in 99.662 CPU sec, 3.224 real sec [M::process] read 704264 sequences (100000150 bp) in 3.525 CPU sec, 3.699 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (113, 285488, 40, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2017) [M::mem_pestat] mean and std.dev: (493.64, 516.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 235, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (265.24, 140.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (420, 629, 2146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5598) [M::mem_pestat] mean and std.dev: (1281.66, 1417.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7324) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 367, 754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1802) [M::mem_pestat] mean and std.dev: (443.22, 365.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704264 reads in 100.753 CPU sec, 3.226 real sec sorting block 55, #reads = 1078895 mem = 536878353 peak = 1021329408 [M::process] read 703476 sequences (100000095 bp) in 3.203 CPU sec, 3.332 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (115, 284819, 38, 138) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 369, 960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2438) [M::mem_pestat] mean and std.dev: (601.33, 576.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 237, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (266.56, 140.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 632, 2980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8420) [M::mem_pestat] mean and std.dev: (1765.50, 2194.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11140) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 362, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561) [M::mem_pestat] mean and std.dev: (422.49, 316.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703476 reads in 102.012 CPU sec, 3.270 real sec [M::process] read 702806 sequences (100000041 bp) in 3.301 CPU sec, 3.447 real sec... sorting block 56, #reads = 1078722 mem = 536878579 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 284680, 64, 113) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 378, 945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2469) [M::mem_pestat] mean and std.dev: (551.15, 542.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 239, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (269.09, 141.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (297, 872, 3163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8895) [M::mem_pestat] mean and std.dev: (2057.91, 2661.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12705) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 304, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1769) [M::mem_pestat] mean and std.dev: (384.19, 355.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2294) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702806 reads in 105.663 CPU sec, 3.388 real sec [M::process] read 702044 sequences (100000187 bp) in 3.208 CPU sec, 3.449 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (115, 283946, 52, 114) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 395, 1376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3646) [M::mem_pestat] mean and std.dev: (718.61, 825.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 241, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (269.63, 140.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (269, 852, 2386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6620) [M::mem_pestat] mean and std.dev: (1306.06, 1280.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8737) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 290, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (296.08, 131.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701926 sequences (100000112 bp) in 3.173 CPU sec, 3.330 real sec... [M::mem_process_seqs] Processed 702044 reads in 105.208 CPU sec, 3.363 real sec sorting block 57, #reads = 1077309 mem = 536880495 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (102, 284224, 49, 128) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 288, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139) [M::mem_pestat] mean and std.dev: (325.12, 208.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 242, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (270.66, 140.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (426, 913, 1964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5040) [M::mem_pestat] mean and std.dev: (1126.27, 953.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6578) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 327, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1398) [M::mem_pestat] mean and std.dev: (350.16, 255.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1797) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701266 sequences (100000138 bp) in 3.092 CPU sec, 3.177 real sec... [M::mem_process_seqs] Processed 701926 reads in 106.023 CPU sec, 3.394 real sec sorting block 58, #reads = 1074706 mem = 536877840 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (104, 283362, 33, 117) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 266, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2005) [M::mem_pestat] mean and std.dev: (376.20, 345.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 243, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (271.80, 141.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (315, 548, 1222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3036) [M::mem_pestat] mean and std.dev: (740.44, 612.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3943) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 287, 672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1582) [M::mem_pestat] mean and std.dev: (337.69, 253.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2037) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701266 reads in 101.592 CPU sec, 3.265 real sec [M::process] read 701618 sequences (100000006 bp) in 3.386 CPU sec, 3.537 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (99, 283986, 47, 107) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 282, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (267.05, 116.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1228) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 241, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (269.58, 140.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (320, 702, 1952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5216) [M::mem_pestat] mean and std.dev: (960.57, 983.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6848) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 404, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2500) [M::mem_pestat] mean and std.dev: (529.22, 510.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701618 reads in 103.674 CPU sec, 3.332 real sec sorting block 59, #reads = 1073654 mem = 536880595 peak = 1021329408 [M::process] read 700852 sequences (100000081 bp) in 3.562 CPU sec, 3.752 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (112, 283583, 48, 128) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 233, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256) [M::mem_pestat] mean and std.dev: (319.55, 270.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 243, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (271.59, 140.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (264, 542, 1258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3246) [M::mem_pestat] mean and std.dev: (680.95, 633.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4240) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 340, 897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2221) [M::mem_pestat] mean and std.dev: (460.58, 411.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700840 sequences (100000272 bp) in 2.729 CPU sec, 2.850 real sec... [M::mem_process_seqs] Processed 700852 reads in 106.150 CPU sec, 3.402 real sec sorting block 60, #reads = 1074821 mem = 536881350 peak = 1021329408 [M::process] read 700872 sequences (100000012 bp) in 2.545 CPU sec, 2.667 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 284023, 57, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 282, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1209) [M::mem_pestat] mean and std.dev: (336.09, 237.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 243, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (270.87, 140.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (277, 801, 1902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5152) [M::mem_pestat] mean and std.dev: (1129.17, 1078.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6777) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 281, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1121) [M::mem_pestat] mean and std.dev: (315.88, 172.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1425) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700626 sequences (100000147 bp) in 1.709 CPU sec, 1.711 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 283894, 43, 118) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 234, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1634) [M::mem_pestat] mean and std.dev: (320.67, 292.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 242, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (270.20, 139.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 677, 2120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5892) [M::mem_pestat] mean and std.dev: (1104.83, 1127.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7778) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 354, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1426) [M::mem_pestat] mean and std.dev: (363.75, 244.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700744 sequences (100000166 bp) in 1.500 CPU sec, 1.502 real sec... [M::mem_process_seqs] Processed 700840 reads in 186.349 CPU sec, 5.873 real sec [M::mem_process_seqs] Processed 700872 reads in 125.459 CPU sec, 3.970 real sec sorting block 61, #reads = 1074271 mem = 536880570 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (109, 283680, 40, 103) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 278, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133) [M::mem_pestat] mean and std.dev: (301.52, 176.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (270.96, 139.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (453, 729, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6897) [M::mem_pestat] mean and std.dev: (1072.17, 1162.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9045) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 311, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1202) [M::mem_pestat] mean and std.dev: (354.82, 225.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1537) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700786 sequences (100000090 bp) in 2.875 CPU sec, 3.015 real sec... [M::process] read 700448 sequences (100000045 bp) in 2.020 CPU sec, 2.017 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (121, 283231, 52, 134) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 277, 791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2053) [M::mem_pestat] mean and std.dev: (452.79, 415.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 243, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (271.18, 140.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (264, 664, 1306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3390) [M::mem_pestat] mean and std.dev: (692.85, 720.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 321, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1287) [M::mem_pestat] mean and std.dev: (347.84, 225.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700626 reads in 190.755 CPU sec, 6.028 real sec [M::mem_process_seqs] Processed 700744 reads in 128.445 CPU sec, 4.069 real sec sorting block 62, #reads = 1074487 mem = 536881778 peak = 1021329408 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (115, 284196, 44, 97) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 313, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1753) [M::mem_pestat] mean and std.dev: (455.33, 372.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 243, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (271.64, 141.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (303, 598, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2871) [M::mem_pestat] mean and std.dev: (710.30, 602.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3727) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 299, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1253) [M::mem_pestat] mean and std.dev: (341.36, 244.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1604) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 708584 sequences (100000144 bp) in 2.702 CPU sec, 2.885 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 283128, 43, 102) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 301, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1772) [M::mem_pestat] mean and std.dev: (430.52, 399.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (274.00, 142.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (255, 672, 2062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5676) [M::mem_pestat] mean and std.dev: (1251.64, 1423.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7483) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 306, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1147) [M::mem_pestat] mean and std.dev: (301.76, 164.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1464) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 713794 sequences (100000261 bp) in 2.280 CPU sec, 2.280 real sec... [M::mem_process_seqs] Processed 700786 reads in 183.434 CPU sec, 5.789 real sec sorting block 63, #reads = 1073837 mem = 536886239 peak = 1021329408 [M::mem_process_seqs] Processed 700448 reads in 124.962 CPU sec, 3.959 real sec sorting block 64, #reads = 1073493 mem = 536881415 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (119, 286934, 35, 92) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 290, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231) [M::mem_pestat] mean and std.dev: (327.21, 259.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 222, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (250.93, 131.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (242, 716, 1658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4490) [M::mem_pestat] mean and std.dev: (1048.03, 1041.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5906) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 315, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1525) [M::mem_pestat] mean and std.dev: (383.02, 306.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1961) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 711916 sequences (100000176 bp) in 3.016 CPU sec, 3.102 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (95, 288198, 40, 90) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 288, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1918) [M::mem_pestat] mean and std.dev: (445.95, 449.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 210, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (235.95, 120.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (232, 875, 2090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5806) [M::mem_pestat] mean and std.dev: (1172.76, 1362.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7664) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 264, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1895) [M::mem_pestat] mean and std.dev: (381.07, 350.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2467) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 709830 sequences (100000066 bp) in 2.400 CPU sec, 2.396 real sec... [M::mem_process_seqs] Processed 708584 reads in 193.755 CPU sec, 6.113 real sec [M::mem_process_seqs] Processed 713794 reads in 131.379 CPU sec, 4.155 real sec sorting block 65, #reads = 1076575 mem = 536878093 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (105, 287457, 42, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 267, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1956) [M::mem_pestat] mean and std.dev: (418.99, 404.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 214, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (241.05, 124.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (177, 606, 1249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3393) [M::mem_pestat] mean and std.dev: (792.62, 751.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4465) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 307, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2270) [M::mem_pestat] mean and std.dev: (460.46, 474.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 708838 sequences (100000019 bp) in 3.487 CPU sec, 3.649 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (98, 287087, 42, 95) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 276, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559) [M::mem_pestat] mean and std.dev: (371.72, 324.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 220, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (247.32, 128.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (153, 595, 2130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6084) [M::mem_pestat] mean and std.dev: (1077.54, 1197.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8061) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 292, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1141) [M::mem_pestat] mean and std.dev: (308.65, 189.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1455) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707812 sequences (100000127 bp) in 2.490 CPU sec, 2.487 real sec... [M::mem_process_seqs] Processed 711916 reads in 198.837 CPU sec, 6.267 real sec sorting block 66, #reads = 1076186 mem = 536879040 peak = 1065615360 [M::mem_process_seqs] Processed 709830 reads in 133.893 CPU sec, 4.222 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 286994, 31, 87) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 302, 1529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4159) [M::mem_pestat] mean and std.dev: (759.94, 858.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 221, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (249.84, 129.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (602, 1286, 2399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5993) [M::mem_pestat] mean and std.dev: (1589.87, 1290.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7790) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 342, 1227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3225) [M::mem_pestat] mean and std.dev: (663.99, 754.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4224) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707314 sequences (100000031 bp) in 3.127 CPU sec, 3.268 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 286068, 32, 115) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 294, 1164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3152) [M::mem_pestat] mean and std.dev: (557.06, 645.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4146) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 224, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (253.47, 132.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (361, 631, 1244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3010) [M::mem_pestat] mean and std.dev: (787.07, 727.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3893) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 340, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1663) [M::mem_pestat] mean and std.dev: (368.70, 264.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 708838 reads in 192.763 CPU sec, 6.085 real sec [M::process] read 707032 sequences (100000123 bp) in 2.596 CPU sec, 2.604 real sec... sorting block 67, #reads = 1076500 mem = 536880592 peak = 1065615360 [M::mem_process_seqs] Processed 707812 reads in 130.245 CPU sec, 4.092 real sec sorting block 68, #reads = 1075544 mem = 536878669 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 285949, 47, 115) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 395, 1074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2790) [M::mem_pestat] mean and std.dev: (640.86, 599.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 226, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (254.95, 133.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (309, 729, 1376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3510) [M::mem_pestat] mean and std.dev: (687.02, 537.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4577) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 342, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1910) [M::mem_pestat] mean and std.dev: (397.97, 320.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2475) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705544 sequences (100000045 bp) in 3.559 CPU sec, 3.725 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (120, 285753, 29, 85) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 326, 691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1727) [M::mem_pestat] mean and std.dev: (361.05, 270.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2245) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 228, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (256.66, 133.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (161, 687, 4190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12248) [M::mem_pestat] mean and std.dev: (1820.48, 2539.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16277) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 251, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1071) [M::mem_pestat] mean and std.dev: (268.86, 152.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1372) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 707314 reads in 189.147 CPU sec, 5.974 real sec [M::mem_process_seqs] Processed 707032 reads in 129.237 CPU sec, 4.055 real sec [M::process] read 704276 sequences (100000066 bp) in 2.753 CPU sec, 2.787 real sec... sorting block 69, #reads = 1077200 mem = 536882617 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 285367, 55, 125) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 302, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (297.37, 180.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 232, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (261.25, 136.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 1019, 3427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9781) [M::mem_pestat] mean and std.dev: (1894.65, 2154.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12958) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 347, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1931) [M::mem_pestat] mean and std.dev: (478.54, 409.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702970 sequences (100000206 bp) in 3.298 CPU sec, 3.490 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (113, 284507, 48, 114) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (249, 518, 1268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3306) [M::mem_pestat] mean and std.dev: (703.85, 765.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4325) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 235, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (264.49, 138.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (395, 972, 2990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8180) [M::mem_pestat] mean and std.dev: (1453.39, 1503.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10775) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 370, 576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1292) [M::mem_pestat] mean and std.dev: (342.62, 200.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 705544 reads in 193.076 CPU sec, 6.152 real sec [M::mem_process_seqs] Processed 704276 reads in 134.152 CPU sec, 4.223 real sec [M::process] read 702164 sequences (100000081 bp) in 2.935 CPU sec, 3.080 real sec... sorting block 70, #reads = 1075580 mem = 536881524 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (110, 284345, 52, 122) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 339, 889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2311) [M::mem_pestat] mean and std.dev: (474.58, 428.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 238, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (267.16, 139.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1144, 2794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7080) [M::mem_pestat] mean and std.dev: (1864.94, 1754.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 359, 1063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2813) [M::mem_pestat] mean and std.dev: (601.32, 645.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3688) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701674 sequences (100000012 bp) in 2.989 CPU sec, 3.103 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (95, 284085, 44, 93) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 318, 899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2275) [M::mem_pestat] mean and std.dev: (500.08, 502.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 240, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (267.76, 138.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (442, 808, 1945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4951) [M::mem_pestat] mean and std.dev: (1219.67, 1254.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6454) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 431, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2196) [M::mem_pestat] mean and std.dev: (433.46, 333.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702970 reads in 193.809 CPU sec, 6.152 real sec sorting block 71, #reads = 1073605 mem = 536880450 peak = 1065615360 [M::mem_process_seqs] Processed 702164 reads in 132.494 CPU sec, 4.179 real sec [M::process] read 701214 sequences (100000215 bp) in 3.032 CPU sec, 3.116 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 283808, 41, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 339, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1869) [M::mem_pestat] mean and std.dev: (424.48, 334.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 241, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (269.29, 140.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (508, 717, 1306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2902) [M::mem_pestat] mean and std.dev: (814.76, 548.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3700) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 297, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561) [M::mem_pestat] mean and std.dev: (370.66, 305.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 72, #reads = 1073545 mem = 536881740 peak = 1065615360 [M::process] read 701852 sequences (100000298 bp) in 3.340 CPU sec, 3.530 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (114, 283568, 35, 119) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 340, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3041) [M::mem_pestat] mean and std.dev: (640.12, 646.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3995) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 242, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (270.21, 140.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (287, 983, 2192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6002) [M::mem_pestat] mean and std.dev: (1222.58, 1106.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7907) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 296, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1301) [M::mem_pestat] mean and std.dev: (309.28, 181.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701674 reads in 191.335 CPU sec, 6.071 real sec [M::mem_process_seqs] Processed 701214 reads in 130.408 CPU sec, 4.105 real sec [M::process] read 700440 sequences (100000251 bp) in 3.193 CPU sec, 3.327 real sec... sorting block 73, #reads = 1074955 mem = 536882541 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 284237, 40, 97) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 289, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298) [M::mem_pestat] mean and std.dev: (314.71, 219.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 240, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (268.64, 139.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (182, 975, 2949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8483) [M::mem_pestat] mean and std.dev: (1366.03, 1437.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11250) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 329, 848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2094) [M::mem_pestat] mean and std.dev: (444.00, 390.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2717) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700110 sequences (100000177 bp) in 3.258 CPU sec, 3.395 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (102, 283370, 38, 150) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 364, 706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1720) [M::mem_pestat] mean and std.dev: (420.20, 325.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2227) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 244, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (272.16, 141.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 652, 1955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5255) [M::mem_pestat] mean and std.dev: (1181.00, 1207.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6905) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 256, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1485) [M::mem_pestat] mean and std.dev: (362.07, 284.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1921) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701852 reads in 189.258 CPU sec, 6.014 real sec [M::mem_process_seqs] Processed 700440 reads in 130.259 CPU sec, 4.096 real sec sorting block 74, #reads = 1074261 mem = 536878246 peak = 1065615360 [M::process] read 700978 sequences (100000239 bp) in 3.278 CPU sec, 3.424 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 283721, 27, 116) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 333, 906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2390) [M::mem_pestat] mean and std.dev: (470.81, 465.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3132) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (273.54, 141.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 546, 1943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5513) [M::mem_pestat] mean and std.dev: (1128.00, 1324.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 306, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1971) [M::mem_pestat] mean and std.dev: (454.77, 410.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2559) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705272 sequences (100000168 bp) in 3.499 CPU sec, 3.674 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 283417, 38, 109) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 300, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (274.46, 124.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1126) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 243, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (271.84, 141.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (186, 1120, 1693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4707) [M::mem_pestat] mean and std.dev: (1046.83, 1014.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 272, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (359.72, 260.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1577) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700110 reads in 187.463 CPU sec, 5.965 real sec sorting block 75, #reads = 1074120 mem = 536882616 peak = 1065615360 [M::mem_process_seqs] Processed 700978 reads in 129.817 CPU sec, 4.094 real sec [M::process] read 711634 sequences (100000076 bp) in 3.581 CPU sec, 3.764 real sec... sorting block 76, #reads = 1074707 mem = 536881135 peak = 1065615360 [M::process] read 710070 sequences (100000242 bp) in 3.145 CPU sec, 3.252 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 287305, 35, 105) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 361, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1115) [M::mem_pestat] mean and std.dev: (335.70, 200.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 216, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (243.19, 126.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (262, 692, 1572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4192) [M::mem_pestat] mean and std.dev: (1098.41, 1071.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 299, 1001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2613) [M::mem_pestat] mean and std.dev: (521.98, 556.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3419) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 711634 reads in 104.834 CPU sec, 3.295 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (100, 284629, 56, 115) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 299, 1044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2776) [M::mem_pestat] mean and std.dev: (524.80, 564.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 231, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (259.75, 136.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (325, 780, 2228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6034) [M::mem_pestat] mean and std.dev: (1244.20, 1271.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7937) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 291, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1236) [M::mem_pestat] mean and std.dev: (346.95, 221.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1583) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 286826, 27, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 356, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1520) [M::mem_pestat] mean and std.dev: (361.09, 275.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 708458 sequences (100000103 bp) in 3.311 CPU sec, 3.441 real sec... [M::mem_pestat] (25, 50, 75) percentile: (147, 220, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (248.05, 129.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (314, 885, 1734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4574) [M::mem_pestat] mean and std.dev: (1261.31, 1374.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6760) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 234, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (268.94, 186.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 705272 reads in 303.164 CPU sec, 9.603 real sec sorting block 77, #reads = 1078531 mem = 536881247 peak = 1065615360 [M::mem_process_seqs] Processed 710070 reads in 153.255 CPU sec, 4.831 real sec [M::process] read 707084 sequences (100000255 bp) in 3.486 CPU sec, 3.649 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 286596, 33, 87) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 278, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (278.32, 144.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1250) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 225, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (252.63, 131.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (849, 1619, 3221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7965) [M::mem_pestat] mean and std.dev: (1701.83, 1471.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10337) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 236, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2201) [M::mem_pestat] mean and std.dev: (482.28, 540.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 78, #reads = 1075809 mem = 536890176 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (105, 285745, 47, 87) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 322, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1448) [M::mem_pestat] mean and std.dev: (394.44, 281.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1871) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 227, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (255.23, 132.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (223, 1060, 2642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7480) [M::mem_pestat] mean and std.dev: (1512.30, 1371.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9899) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 261, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (257.23, 140.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1349) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705898 sequences (100000107 bp) in 3.033 CPU sec, 3.141 real sec... [M::mem_process_seqs] Processed 708458 reads in 178.541 CPU sec, 5.680 real sec [M::mem_process_seqs] Processed 707084 reads in 122.983 CPU sec, 3.884 real sec sorting block 79, #reads = 1082409 mem = 536877596 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 285924, 41, 99) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 251, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1633) [M::mem_pestat] mean and std.dev: (351.00, 326.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 231, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (258.83, 134.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 813, 4804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13860) [M::mem_pestat] mean and std.dev: (2669.05, 3220.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 313, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231) [M::mem_pestat] mean and std.dev: (357.97, 241.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705356 sequences (100000111 bp) in 3.927 CPU sec, 4.211 real sec... [M::mem_process_seqs] Processed 705898 reads in 102.401 CPU sec, 3.371 real sec sorting block 80, #reads = 1080281 mem = 536877945 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (109, 285744, 38, 98) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 223, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (254.90, 179.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 232, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (260.43, 135.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (368, 699, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3812) [M::mem_pestat] mean and std.dev: (886.46, 795.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4960) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 274, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1329) [M::mem_pestat] mean and std.dev: (320.21, 239.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1712) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705438 sequences (100000229 bp) in 3.565 CPU sec, 3.793 real sec... [M::mem_process_seqs] Processed 705356 reads in 116.215 CPU sec, 3.754 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 285378, 43, 95) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 371, 1020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2680) [M::mem_pestat] mean and std.dev: (515.84, 500.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 232, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (259.69, 134.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (443, 818, 2718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7268) [M::mem_pestat] mean and std.dev: (1579.09, 1677.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9543) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 374, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1214) [M::mem_pestat] mean and std.dev: (336.57, 175.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705296 sequences (100000257 bp) in 3.362 CPU sec, 3.535 real sec... [M::mem_process_seqs] Processed 705438 reads in 104.714 CPU sec, 3.360 real sec sorting block 81, #reads = 1077836 mem = 536878365 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 285430, 42, 91) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 353, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1510) [M::mem_pestat] mean and std.dev: (411.84, 306.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 232, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (260.27, 134.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (262, 619, 1494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3958) [M::mem_pestat] mean and std.dev: (749.58, 666.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5190) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 237, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (252.16, 129.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 705296 reads in 104.707 CPU sec, 3.352 real sec [M::process] read 704480 sequences (100000298 bp) in 3.461 CPU sec, 3.656 real sec... sorting block 82, #reads = 1079078 mem = 536882465 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (109, 284945, 43, 116) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 288, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1354) [M::mem_pestat] mean and std.dev: (295.04, 175.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 235, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (262.24, 135.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (708, 1145, 2706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6702) [M::mem_pestat] mean and std.dev: (1979.07, 2026.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10084) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 292, 672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1662) [M::mem_pestat] mean and std.dev: (452.48, 432.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2184) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704480 reads in 105.274 CPU sec, 3.373 real sec [M::process] read 705034 sequences (100000293 bp) in 3.224 CPU sec, 3.378 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 285420, 52, 92) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 311, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1191) [M::mem_pestat] mean and std.dev: (341.94, 238.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 233, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (259.84, 133.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (536, 1113, 3152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8384) [M::mem_pestat] mean and std.dev: (1769.24, 1720.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11000) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 291, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2336) [M::mem_pestat] mean and std.dev: (448.87, 405.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3044) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704244 sequences (100000156 bp) in 2.895 CPU sec, 2.990 real sec... [M::mem_process_seqs] Processed 705034 reads in 103.704 CPU sec, 3.326 real sec sorting block 83, #reads = 1077983 mem = 536888301 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (116, 284559, 44, 116) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 311, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2601) [M::mem_pestat] mean and std.dev: (499.38, 547.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 237, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (265.02, 138.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (127, 605, 1609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4573) [M::mem_pestat] mean and std.dev: (757.40, 757.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6055) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 380, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1809) [M::mem_pestat] mean and std.dev: (431.70, 327.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2337) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702892 sequences (100000143 bp) in 3.348 CPU sec, 3.538 real sec... [M::mem_process_seqs] Processed 704244 reads in 112.486 CPU sec, 3.601 real sec sorting block 84, #reads = 1077316 mem = 536877666 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (116, 284695, 34, 99) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 282, 914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2350) [M::mem_pestat] mean and std.dev: (446.94, 440.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3068) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 239, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (267.37, 138.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (325, 1108, 2221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6013) [M::mem_pestat] mean and std.dev: (1426.47, 1282.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7909) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 329, 725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1819) [M::mem_pestat] mean and std.dev: (426.08, 372.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2366) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701916 sequences (100000174 bp) in 3.356 CPU sec, 3.520 real sec... [M::mem_process_seqs] Processed 702892 reads in 110.293 CPU sec, 3.532 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 283814, 33, 124) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 315, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1390) [M::mem_pestat] mean and std.dev: (337.51, 244.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 241, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (269.66, 140.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 1649, 2566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7088) [M::mem_pestat] mean and std.dev: (1428.87, 1346.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 357, 698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1644) [M::mem_pestat] mean and std.dev: (449.28, 362.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2117) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702524 sequences (100000200 bp) in 3.330 CPU sec, 3.512 real sec... [M::mem_process_seqs] Processed 701916 reads in 109.904 CPU sec, 3.521 real sec sorting block 85, #reads = 1075696 mem = 536879600 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (102, 284260, 39, 121) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 367, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2580) [M::mem_pestat] mean and std.dev: (599.85, 612.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 237, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (265.64, 137.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (537, 1186, 2582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6672) [M::mem_pestat] mean and std.dev: (1485.05, 1344.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 323, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1830) [M::mem_pestat] mean and std.dev: (437.90, 354.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701366 sequences (100000255 bp) in 3.361 CPU sec, 3.536 real sec... [M::mem_process_seqs] Processed 702524 reads in 113.688 CPU sec, 3.629 real sec sorting block 86, #reads = 1074053 mem = 536877251 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (128, 283342, 57, 117) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 281, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1497) [M::mem_pestat] mean and std.dev: (339.74, 272.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (267.84, 137.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (352, 589, 1682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4342) [M::mem_pestat] mean and std.dev: (810.53, 918.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 317, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1952) [M::mem_pestat] mean and std.dev: (425.16, 377.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2531) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701744 sequences (100000096 bp) in 3.365 CPU sec, 3.540 real sec... [M::mem_process_seqs] Processed 701366 reads in 115.732 CPU sec, 3.707 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 283861, 50, 119) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 291, 930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2392) [M::mem_pestat] mean and std.dev: (400.52, 322.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 241, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (269.38, 140.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (271, 605, 1816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4906) [M::mem_pestat] mean and std.dev: (1000.71, 1012.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6451) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 359, 848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2176) [M::mem_pestat] mean and std.dev: (499.59, 469.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2840) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701658 sequences (100000235 bp) in 3.301 CPU sec, 3.443 real sec... [M::mem_process_seqs] Processed 701744 reads in 110.784 CPU sec, 3.535 real sec sorting block 87, #reads = 1073074 mem = 536877658 peak = 1065615360 [M::process] read 701316 sequences (100000265 bp) in 3.253 CPU sec, 3.395 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (113, 283828, 29, 85) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 297, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (298.77, 169.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 241, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (269.62, 139.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (678, 1044, 2197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5235) [M::mem_pestat] mean and std.dev: (1318.65, 1190.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6754) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 291, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319) [M::mem_pestat] mean and std.dev: (357.92, 258.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1694) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706508 sequences (100000125 bp) in 2.250 CPU sec, 2.248 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (113, 283613, 42, 126) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 302, 1148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3048) [M::mem_pestat] mean and std.dev: (515.42, 551.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (268.67, 139.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (329, 878, 1938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5156) [M::mem_pestat] mean and std.dev: (1168.15, 1027.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6765) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 492, 1702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4686) [M::mem_pestat] mean and std.dev: (935.75, 1118.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6178) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701658 reads in 194.137 CPU sec, 6.094 real sec [M::process] read 716160 sequences (100000078 bp) in 2.681 CPU sec, 2.791 real sec... sorting block 88, #reads = 1071668 mem = 536883330 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 285100, 42, 106) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 382, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1855) [M::mem_pestat] mean and std.dev: (408.54, 352.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 228, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (256.41, 134.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (229, 560, 1647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4483) [M::mem_pestat] mean and std.dev: (806.66, 850.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5901) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 321, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1090) [M::mem_pestat] mean and std.dev: (301.86, 175.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 713570 sequences (100000171 bp) in 2.318 CPU sec, 2.324 real sec... [M::mem_process_seqs] Processed 706508 reads in 128.284 CPU sec, 4.052 real sec [M::mem_process_seqs] Processed 701316 reads in 278.108 CPU sec, 8.775 real sec sorting block 89, #reads = 1067415 mem = 536882697 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 289182, 25, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 284, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (227.55, 98.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (140, 206, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (231.65, 118.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (482, 1021, 2796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7424) [M::mem_pestat] mean and std.dev: (1759.80, 1580.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9738) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 311, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2171) [M::mem_pestat] mean and std.dev: (434.68, 354.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 711960 sequences (100000276 bp) in 3.013 CPU sec, 3.113 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (107, 288004, 36, 97) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 348, 1074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2826) [M::mem_pestat] mean and std.dev: (539.51, 550.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 212, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (239.27, 123.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (314, 1529, 2523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6941) [M::mem_pestat] mean and std.dev: (1558.79, 1501.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 331, 682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1624) [M::mem_pestat] mean and std.dev: (412.20, 317.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 709958 sequences (100000210 bp) in 2.316 CPU sec, 2.316 real sec... [M::mem_process_seqs] Processed 716160 reads in 232.858 CPU sec, 7.357 real sec [M::mem_process_seqs] Processed 713570 reads in 138.255 CPU sec, 4.362 real sec sorting block 90, #reads = 1075726 mem = 536879232 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 287929, 47, 90) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 288, 1071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2871) [M::mem_pestat] mean and std.dev: (588.20, 712.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3771) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (144, 216, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (243.13, 125.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (417, 705, 1254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2928) [M::mem_pestat] mean and std.dev: (666.34, 469.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3765) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 331, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1449) [M::mem_pestat] mean and std.dev: (352.16, 242.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 708542 sequences (100000198 bp) in 2.980 CPU sec, 3.058 real sec... [M::process] read 706902 sequences (100000137 bp) in 1.710 CPU sec, 1.718 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (109, 287482, 23, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 301, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1553) [M::mem_pestat] mean and std.dev: (357.96, 305.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 220, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (248.45, 128.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 871) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (486, 725, 1619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3885) [M::mem_pestat] mean and std.dev: (922.50, 599.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5018) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 301, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1410) [M::mem_pestat] mean and std.dev: (372.83, 281.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1806) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 711960 reads in 204.655 CPU sec, 6.493 real sec sorting block 91, #reads = 1076501 mem = 536883414 peak = 1065615360 [M::mem_process_seqs] Processed 709958 reads in 138.643 CPU sec, 4.420 real sec sorting block 92, #reads = 1076202 mem = 536876753 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (98, 286393, 45, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 422, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3394) [M::mem_pestat] mean and std.dev: (789.99, 844.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 224, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (252.59, 131.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1205, 1935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4547) [M::mem_pestat] mean and std.dev: (1256.83, 959.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5853) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 301, 885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2259) [M::mem_pestat] mean and std.dev: (475.71, 435.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2946) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707290 sequences (100000152 bp) in 2.706 CPU sec, 2.854 real sec... [M::process] read 706556 sequences (100000211 bp) in 1.930 CPU sec, 1.933 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 286002, 48, 100) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 303, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1726) [M::mem_pestat] mean and std.dev: (376.70, 344.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 228, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (257.53, 134.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 911) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (67, 783, 2151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6319) [M::mem_pestat] mean and std.dev: (1231.33, 1347.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8403) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 320, 957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2489) [M::mem_pestat] mean and std.dev: (513.96, 520.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3255) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 708542 reads in 201.052 CPU sec, 6.375 real sec [M::mem_process_seqs] Processed 706902 reads in 136.458 CPU sec, 4.335 real sec sorting block 93, #reads = 1075198 mem = 536880414 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 285523, 37, 85) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 380, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (366.52, 231.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 230, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (259.10, 135.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (111, 474, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3156) [M::mem_pestat] mean and std.dev: (727.28, 813.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4171) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 299, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1408) [M::mem_pestat] mean and std.dev: (339.76, 217.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707262 sequences (100000063 bp) in 3.057 CPU sec, 3.144 real sec... [M::process] read 703974 sequences (100000120 bp) in 2.520 CPU sec, 2.524 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (118, 285950, 54, 96) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 380, 971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2609) [M::mem_pestat] mean and std.dev: (532.33, 544.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 226, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (255.53, 133.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (207, 1027, 2152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6042) [M::mem_pestat] mean and std.dev: (1264.75, 1280.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7987) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 333, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1907) [M::mem_pestat] mean and std.dev: (487.10, 448.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 706556 reads in 204.310 CPU sec, 6.451 real sec sorting block 94, #reads = 1074638 mem = 536885525 peak = 1065615360 [M::mem_process_seqs] Processed 707290 reads in 231.955 CPU sec, 7.355 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (114, 285780, 49, 105) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 349, 1389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3807) [M::mem_pestat] mean and std.dev: (621.68, 871.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5016) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 225, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (253.51, 131.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (656, 1913, 3879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10325) [M::mem_pestat] mean and std.dev: (2513.10, 2434.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13548) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 318, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1257) [M::mem_pestat] mean and std.dev: (309.87, 162.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702384 sequences (100000001 bp) in 3.341 CPU sec, 3.475 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (109, 284603, 49, 95) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 281, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1530) [M::mem_pestat] mean and std.dev: (386.51, 307.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 235, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (262.56, 135.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (465, 872, 1955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4935) [M::mem_pestat] mean and std.dev: (1074.48, 853.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6425) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 273, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (301.80, 198.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1419) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701624 sequences (100000189 bp) in 2.540 CPU sec, 2.541 real sec... [M::mem_process_seqs] Processed 707262 reads in 206.563 CPU sec, 6.515 real sec sorting block 95, #reads = 1073571 mem = 536880908 peak = 1065615360 [M::mem_process_seqs] Processed 703974 reads in 234.156 CPU sec, 7.418 real sec sorting block 96, #reads = 1073885 mem = 536880276 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (116, 283664, 39, 128) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 276, 690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1714) [M::mem_pestat] mean and std.dev: (405.60, 385.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 240, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (266.77, 137.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (397, 818, 2254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5968) [M::mem_pestat] mean and std.dev: (1452.97, 1471.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7825) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 325, 1050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2816) [M::mem_pestat] mean and std.dev: (487.39, 501.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3699) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702098 sequences (100000128 bp) in 3.228 CPU sec, 3.299 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (113, 283010, 55, 132) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 294, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2420) [M::mem_pestat] mean and std.dev: (526.89, 593.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (266.67, 136.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (350, 939, 1514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3842) [M::mem_pestat] mean and std.dev: (971.20, 799.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5006) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 269, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1828) [M::mem_pestat] mean and std.dev: (401.16, 370.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2380) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701192 sequences (100000008 bp) in 2.550 CPU sec, 2.552 real sec... [M::mem_process_seqs] Processed 702384 reads in 197.153 CPU sec, 6.220 real sec [M::mem_process_seqs] Processed 701624 reads in 143.165 CPU sec, 4.540 real sec sorting block 97, #reads = 1072063 mem = 536876255 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 283738, 36, 123) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 305, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1481) [M::mem_pestat] mean and std.dev: (341.38, 236.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 238, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (264.13, 134.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (982, 1571, 2254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4798) [M::mem_pestat] mean and std.dev: (1484.88, 735.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6070) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 338, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1568) [M::mem_pestat] mean and std.dev: (362.13, 279.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700840 sequences (100000083 bp) in 2.905 CPU sec, 3.053 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (96, 283560, 49, 126) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 309, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133) [M::mem_pestat] mean and std.dev: (298.82, 187.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (266.47, 135.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (542, 1296, 3634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9818) [M::mem_pestat] mean and std.dev: (2025.98, 2167.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12910) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 346, 1103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2871) [M::mem_pestat] mean and std.dev: (564.77, 590.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3755) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700506 sequences (100000184 bp) in 2.350 CPU sec, 2.357 real sec... [M::mem_process_seqs] Processed 702098 reads in 188.692 CPU sec, 5.962 real sec [M::mem_process_seqs] Processed 701192 reads in 129.640 CPU sec, 4.091 real sec sorting block 98, #reads = 1074942 mem = 536882299 peak = 1065615360 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (95, 283382, 47, 104) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 294, 1139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3087) [M::mem_pestat] mean and std.dev: (576.93, 637.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (266.77, 135.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 1167, 1653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4477) [M::mem_pestat] mean and std.dev: (1053.09, 889.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5889) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 326, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1621) [M::mem_pestat] mean and std.dev: (440.79, 373.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2088) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699958 sequences (100000129 bp) in 2.947 CPU sec, 3.092 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 283074, 57, 100) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 267, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1365) [M::mem_pestat] mean and std.dev: (361.42, 266.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (271.95, 139.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (472, 1528, 3201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8659) [M::mem_pestat] mean and std.dev: (2206.02, 2151.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 272, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015) [M::mem_pestat] mean and std.dev: (262.22, 130.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700336 sequences (100000251 bp) in 2.321 CPU sec, 2.329 real sec... [M::mem_process_seqs] Processed 700840 reads in 187.019 CPU sec, 5.912 real sec sorting block 99, #reads = 1074636 mem = 536876043 peak = 1065615360 [M::mem_process_seqs] Processed 700506 reads in 129.313 CPU sec, 4.078 real sec [M::process] read 58066 sequences (8276060 bp) in 0.901 CPU sec, 0.913 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 283732, 43, 102) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 323, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2146) [M::mem_pestat] mean and std.dev: (497.28, 469.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 247, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (273.30, 140.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (284, 684, 1542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4058) [M::mem_pestat] mean and std.dev: (765.36, 629.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 392, 891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2267) [M::mem_pestat] mean and std.dev: (456.85, 421.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2955) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (111, 283466, 51, 110) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 322, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1621) [M::mem_pestat] mean and std.dev: (404.37, 347.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (273.14, 141.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (336, 883, 2617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7179) [M::mem_pestat] mean and std.dev: (1206.76, 1240.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9460) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 334, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167) [M::mem_pestat] mean and std.dev: (358.64, 239.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1489) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 23383, 3, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 238, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (265.81, 136.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 699958 reads in 192.154 CPU sec, 6.210 real sec sorting block 100, #reads = 1074251 mem = 536881782 peak = 1065615360 [M::mem_process_seqs] Processed 700336 reads in 137.906 CPU sec, 4.459 real sec [M::mem_process_seqs] Processed 58066 reads in 140.599 CPU sec, 4.554 real sec sorting block 101, #reads = 1075854 mem = 536887032 peak = 1085595648 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: INTEL(R) XEON(R) PLATINUM 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 109053494 overall: 70008815616 mem 18292.040 user 561.176 sys 628.583 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_85O-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:1136_85O-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_85O-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_85O-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_85O-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 628.621 sec; CPU: 18853.220 sec sorting block 102, #reads = 396537 mem = 198967715 peak = 1085595648 uniq header count 1 merging 103 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: INTEL(R) XEON(R) PLATINUM 8559C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 6274341682 output reads: 110221104 bam_mem_sort: 103 calls 55.064 user 1.278 sys 56.557 real bam_write: 103 calls 30.911 user 1.532 sys 33.017 real execute: 1 calls 34.903 user 1.713 sys 675.422 real merge_files: 1 calls 34.855 user 1.702 sys 46.524 real parse_chunk: 1638 calls 88.920 user 21.715 sys 112.483 real read_chunk: 4433 calls 51.797 user 33.894 sys 575.020 real sort_block: 1 calls 34.428 user 33.041 sys 552.017 real write_chunk: 2793 calls 34.408 user 1.418 sys 45.525 real overall: 3355336704 mem 1799.662 user 90.417 sys 675.447 real ls: cannot access '*.cram': No such file or directory