This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o NTC_0002_0015_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2639.467 CPU sec, 110.531 real sec [M::process] read 4974 sequences (749116 bp) in 0.008 CPU sec, 0.008 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 56, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 193, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (236.45, 145.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 4974 reads in 9.844 CPU sec, 0.323 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 4974 overall: 68136468480 mem 2068.921 user 592.346 sys 122.811 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.NTC_0002_0015_A23MHWWLT4_1.L001\tPU:L001\tSM:NTC_0002_0015_A23MHWWLT4_1\tLB:NTC_0002_0015_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 NTC_0002_0015_A23MHWWLT4_1_R1.fastq.gz NTC_0002_0015_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 122.839 sec; CPU: 2661.270 sec sorting block 0, #reads = 5192 mem = 2879528 peak = 75571200 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 380211 output reads: 5192 bam_mem_sort: 1 calls 0.000 user 0.000 sys 0.001 real bam_write: 1 calls 0.000 user 0.005 sys 0.004 real execute: 1 calls 0.043 user 0.143 sys 122.992 real merge_files: 1 calls 0.023 user 0.117 sys 0.159 real parse_chunk: 1 calls 0.000 user 0.000 sys 0.004 real read_chunk: 4 calls 0.003 user 0.000 sys 11.945 real sort_block: 1 calls 0.007 user 0.005 sys 11.950 real write_chunk: 1 calls 0.000 user 0.000 sys 0.002 real overall: 265457664 mem 0.127 user 0.230 sys 123.009 real ls: cannot access '*.cram': No such file or directory