Detecting adapter sequence for read1...
>Illumina TruSeq Adapter Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA

Detecting adapter sequence for read2...
>Illumina TruSeq Adapter Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT

Read1 before filtering:
total reads: 133574782
total bases: 20169792082
Q20 bases: 19599431647(97.1722%)
Q30 bases: 18527293102(91.8566%)
Q40 bases: 18527293102(91.8566%)

Read2 before filtering:
total reads: 133574782
total bases: 20169792082
Q20 bases: 19073918557(94.5668%)
Q30 bases: 17269946755(85.6228%)
Q40 bases: 17269946755(85.6228%)

Read1 after filtering:
total reads: 130622447
total bases: 19145165813
Q20 bases: 18697798932(97.6633%)
Q30 bases: 17753216459(92.7295%)
Q40 bases: 17753216459(92.7295%)

Read2 after filtering:
total reads: 130622447
total bases: 19146510993
Q20 bases: 18302962708(95.5942%)
Q30 bases: 16714697164(87.2989%)
Q40 bases: 16714697164(87.2989%)

Filtering result:
reads passed filter: 261244894
reads failed due to low quality: 5872618
reads failed due to too many N: 25544
reads failed due to too short: 6508
reads with adapter trimmed: 31421474
bases trimmed due to adapters: 1160918607

Duplication rate: 13.8688%

Insert size peak (evaluated by paired-end reads): 271

JSON report: 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.fastp.json
HTML report: 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.fastp.html

fastp --in1 1029_N5D-T1-TDNA-1_A23MHWWLT4_1_S1_L008_R1_001.fastq.gz --in2 1029_N5D-T1-TDNA-1_A23MHWWLT4_1_S1_L008_R2_001.fastq.gz --out1 1029_N5D-T1-TDNA-1_A23MHWWLT4_1/1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz --out2 1029_N5D-T1-TDNA-1_A23MHWWLT4_1/1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz --detect_adapter_for_pe --length_required 15 --json 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.fastp.json --html 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.fastp.html --thread 4 --split_by_lines 400000000 
fastp v1.0.1, time used: 1692 seconds