cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_Kh-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2086.183 CPU sec, 73.055 real sec
[M::process] read 672540 sequences (100000011 bp) in 1.318 CPU sec, 1.326 real sec...
[M::process] read 672242 sequences (100000059 bp) in 1.585 CPU sec, 1.647 real sec...
[M::process] read 672340 sequences (100000065 bp) in 1.620 CPU sec, 1.632 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278088, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 361, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (379.38, 165.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672218 sequences (100000263 bp) in 1.586 CPU sec, 1.600 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278543, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 366, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (383.51, 166.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278804, 7, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 365, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (382.84, 166.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672540 reads in 281.062 CPU sec, 9.123 real sec
[M::mem_process_seqs] Processed 672242 reads in 188.553 CPU sec, 6.201 real sec
[M::mem_process_seqs] Processed 672340 reads in 205.391 CPU sec, 6.764 real sec
sorting block 0, #reads = 1075631 mem = 536879252 peak = 593588224
[M::process] read 671768 sequences (100000278 bp) in 2.098 CPU sec, 2.263 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278838, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 368, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (384.80, 166.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671732 sequences (100000037 bp) in 1.831 CPU sec, 2.011 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278887, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 372, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (388.98, 168.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671160 sequences (100000013 bp) in 1.545 CPU sec, 1.547 real sec...
[M::mem_process_seqs] Processed 672218 reads in 180.867 CPU sec, 5.772 real sec
sorting block 1, #reads = 1075564 mem = 536878995 peak = 862547968
[M::mem_process_seqs] Processed 671768 reads in 114.758 CPU sec, 3.635 real sec
sorting block 2, #reads = 1076060 mem = 536879568 peak = 867803136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279097, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 375, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (391.94, 169.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671118 sequences (100000184 bp) in 2.159 CPU sec, 2.370 real sec...
[M::process] read 670950 sequences (100000129 bp) in 1.774 CPU sec, 1.840 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279292, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (396.69, 170.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671732 reads in 177.661 CPU sec, 5.765 real sec
[M::mem_process_seqs] Processed 671160 reads in 112.654 CPU sec, 3.687 real sec
sorting block 3, #reads = 1075385 mem = 536879201 peak = 867803136
[M::process] read 670758 sequences (100000076 bp) in 2.226 CPU sec, 2.681 real sec...
[M::process] read 670840 sequences (100000164 bp) in 1.782 CPU sec, 1.785 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278178, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 387, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (402.03, 172.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670950 reads in 99.127 CPU sec, 3.195 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278502, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 384, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (399.18, 170.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1075932 mem = 536878462 peak = 867803136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278100, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 389, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (404.83, 173.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670886 sequences (100000264 bp) in 1.754 CPU sec, 1.809 real sec...
[M::mem_process_seqs] Processed 671118 reads in 277.454 CPU sec, 9.085 real sec
[M::mem_process_seqs] Processed 670758 reads in 131.758 CPU sec, 4.345 real sec
sorting block 5, #reads = 1075387 mem = 536879728 peak = 867803136
[M::process] read 670756 sequences (100000296 bp) in 2.114 CPU sec, 2.420 real sec...
[M::process] read 670782 sequences (100000078 bp) in 1.821 CPU sec, 1.983 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277960, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 387, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (401.81, 172.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670886 reads in 101.919 CPU sec, 3.371 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278029, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 387, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (402.67, 172.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277850, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 384, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (399.41, 170.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670902 sequences (100000037 bp) in 1.931 CPU sec, 1.935 real sec...
[M::mem_process_seqs] Processed 670840 reads in 286.760 CPU sec, 9.427 real sec
sorting block 6, #reads = 1075585 mem = 536881534 peak = 867803136
[M::mem_process_seqs] Processed 670756 reads in 136.517 CPU sec, 4.512 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277288, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 383, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (398.68, 170.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 7, #reads = 1075611 mem = 536880210 peak = 907931648
[M::process] read 670922 sequences (100000266 bp) in 2.288 CPU sec, 2.481 real sec...
[M::process] read 671036 sequences (100000019 bp) in 1.873 CPU sec, 1.876 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277813, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 380, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (396.08, 169.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670782 reads in 187.910 CPU sec, 6.168 real sec
[M::mem_process_seqs] Processed 670902 reads in 120.088 CPU sec, 3.919 real sec
sorting block 8, #reads = 1074716 mem = 536880158 peak = 907931648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277823, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 381, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (397.16, 170.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670822 sequences (100000204 bp) in 2.491 CPU sec, 2.771 real sec...
[M::process] read 670816 sequences (100000157 bp) in 2.007 CPU sec, 2.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278171, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 380, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (396.64, 170.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670922 reads in 193.135 CPU sec, 6.359 real sec
[M::mem_process_seqs] Processed 671036 reads in 123.888 CPU sec, 4.075 real sec
sorting block 9, #reads = 1075054 mem = 536878729 peak = 907931648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278213, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 383, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (399.14, 171.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670672 sequences (100000216 bp) in 2.461 CPU sec, 2.900 real sec...
[M::process] read 670754 sequences (100000056 bp) in 1.728 CPU sec, 1.738 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277681, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (399.84, 171.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670822 reads in 181.887 CPU sec, 5.957 real sec
[M::mem_process_seqs] Processed 670816 reads in 117.018 CPU sec, 3.815 real sec
sorting block 10, #reads = 1075170 mem = 536877932 peak = 907931648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277207, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 386, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (401.87, 171.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670402 sequences (100000148 bp) in 2.458 CPU sec, 2.716 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277687, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 382, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (398.39, 169.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670362 sequences (100000089 bp) in 1.867 CPU sec, 1.955 real sec...
[M::mem_process_seqs] Processed 670672 reads in 182.854 CPU sec, 6.044 real sec
sorting block 11, #reads = 1075902 mem = 536880287 peak = 907931648
[M::mem_process_seqs] Processed 670754 reads in 116.486 CPU sec, 3.813 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277927, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 385, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (400.92, 169.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 12, #reads = 1074661 mem = 536878009 peak = 940752896
[M::process] read 670472 sequences (100000221 bp) in 2.570 CPU sec, 3.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277781, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (401.77, 169.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670166 sequences (100000166 bp) in 1.832 CPU sec, 1.908 real sec...
[M::mem_process_seqs] Processed 670402 reads in 178.717 CPU sec, 5.816 real sec
[M::mem_process_seqs] Processed 670362 reads in 114.259 CPU sec, 3.710 real sec
sorting block 13, #reads = 1075370 mem = 536877979 peak = 940752896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278306, 7, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (401.62, 169.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670146 sequences (100000096 bp) in 2.619 CPU sec, 2.986 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277321, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 390, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (406.16, 172.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670472 reads in 178.239 CPU sec, 5.827 real sec
[M::process] read 669978 sequences (100000117 bp) in 1.827 CPU sec, 2.056 real sec...
[M::mem_process_seqs] Processed 670166 reads in 114.554 CPU sec, 3.723 real sec
sorting block 14, #reads = 1073898 mem = 536879646 peak = 940752896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277484, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 394, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (409.39, 174.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670018 sequences (100000266 bp) in 2.586 CPU sec, 3.245 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277648, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 395, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (410.53, 174.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670146 reads in 173.858 CPU sec, 5.687 real sec
[M::mem_process_seqs] Processed 669978 reads in 112.252 CPU sec, 3.618 real sec
[M::process] read 671382 sequences (100000133 bp) in 1.950 CPU sec, 2.034 real sec...
sorting block 15, #reads = 1075391 mem = 536880140 peak = 940752896
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277574, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (277, 396, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (412.82, 175.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672402 sequences (100000257 bp) in 2.560 CPU sec, 2.991 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278000, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (395.50, 170.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (857, 3382, 3382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8432)
[M::mem_pestat] mean and std.dev: (2624.50, 1157.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10957)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 670018 reads in 174.773 CPU sec, 5.626 real sec
sorting block 16, #reads = 1074621 mem = 536881029 peak = 940752896
[M::process] read 672034 sequences (100000130 bp) in 1.920 CPU sec, 1.940 real sec...
[M::mem_process_seqs] Processed 671382 reads in 113.655 CPU sec, 3.605 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277977, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 370, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (387.05, 168.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 17, #reads = 1073937 mem = 536876874 peak = 959643648
[M::process] read 672046 sequences (100000080 bp) in 2.741 CPU sec, 3.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277376, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 375, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (390.86, 170.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671846 sequences (100000235 bp) in 1.809 CPU sec, 1.817 real sec...
[M::mem_process_seqs] Processed 672402 reads in 178.396 CPU sec, 5.775 real sec
[M::mem_process_seqs] Processed 672034 reads in 112.145 CPU sec, 3.649 real sec
sorting block 18, #reads = 1077607 mem = 536877036 peak = 959643648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277628, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 375, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (390.73, 169.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671832 sequences (100000081 bp) in 2.677 CPU sec, 3.102 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277680, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 377, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (391.60, 168.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671506 sequences (100000129 bp) in 1.847 CPU sec, 1.852 real sec...
[M::mem_process_seqs] Processed 672046 reads in 177.834 CPU sec, 5.745 real sec
[M::mem_process_seqs] Processed 671846 reads in 111.734 CPU sec, 3.590 real sec
sorting block 19, #reads = 1076392 mem = 536879585 peak = 959643648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277894, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 378, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (393.69, 169.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671192 sequences (100000067 bp) in 2.619 CPU sec, 3.091 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277642, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (396.45, 170.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671084 sequences (100000045 bp) in 1.885 CPU sec, 1.890 real sec...
[M::mem_process_seqs] Processed 671832 reads in 176.441 CPU sec, 5.774 real sec
sorting block 20, #reads = 1077137 mem = 536879479 peak = 959643648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278098, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (399.86, 171.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670994 sequences (100000093 bp) in 2.043 CPU sec, 2.064 real sec...
[M::mem_process_seqs] Processed 671192 reads in 100.467 CPU sec, 3.447 real sec
[M::mem_process_seqs] Processed 671506 reads in 254.040 CPU sec, 8.580 real sec
sorting block 21, #reads = 1075795 mem = 536878450 peak = 959643648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278285, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 387, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (401.38, 172.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671050 sequences (100000237 bp) in 2.517 CPU sec, 3.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277461, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 391, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (405.23, 172.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671084 reads in 191.849 CPU sec, 6.271 real sec
[M::mem_process_seqs] Processed 670994 reads in 113.926 CPU sec, 3.703 real sec
sorting block 22, #reads = 1076873 mem = 536879001 peak = 959643648
[M::process] read 670706 sequences (100000029 bp) in 2.277 CPU sec, 2.473 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277859, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 390, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (404.25, 173.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670920 sequences (100000022 bp) in 2.003 CPU sec, 2.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277862, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 394, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (407.37, 174.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671050 reads in 179.801 CPU sec, 5.844 real sec
[M::process] read 670916 sequences (100000023 bp) in 2.018 CPU sec, 2.106 real sec...
[M::mem_process_seqs] Processed 670706 reads in 115.531 CPU sec, 3.723 real sec
sorting block 23, #reads = 1075518 mem = 536881151 peak = 959643648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 277570, 3, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (5503, 5766, 5766)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (4977, 6292)
[M::mem_pestat] mean and std.dev: (5696.92, 110.02)
[M::mem_pestat] low and high boundaries for proper pairs: (4714, 6555)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 391, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (406.31, 174.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 670832 sequences (100000169 bp) in 2.693 CPU sec, 3.169 real sec...
sorting block 24, #reads = 1076856 mem = 536879560 peak = 959643648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 276919, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 389, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (404.15, 174.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670920 reads in 185.620 CPU sec, 6.088 real sec
[M::process] read 671000 sequences (100000190 bp) in 1.973 CPU sec, 2.016 real sec...
[M::mem_process_seqs] Processed 670916 reads in 118.224 CPU sec, 3.859 real sec
sorting block 25, #reads = 1075228 mem = 536881132 peak = 959643648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277618, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 389, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (403.73, 172.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671170 sequences (100000249 bp) in 2.535 CPU sec, 2.942 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 277816, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 380, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (397.17, 170.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671292 sequences (100000138 bp) in 1.952 CPU sec, 1.961 real sec...
[M::mem_process_seqs] Processed 670832 reads in 187.506 CPU sec, 6.033 real sec
[M::mem_process_seqs] Processed 671000 reads in 118.561 CPU sec, 3.786 real sec
sorting block 26, #reads = 1074213 mem = 536879435 peak = 959643648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277928, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 377, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (393.91, 169.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671154 sequences (100000202 bp) in 2.619 CPU sec, 3.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278236, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 376, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (393.16, 169.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671170 reads in 182.404 CPU sec, 5.868 real sec
[M::process] read 670878 sequences (100000110 bp) in 1.931 CPU sec, 2.065 real sec...
[M::mem_process_seqs] Processed 671292 reads in 116.399 CPU sec, 3.730 real sec
sorting block 27, #reads = 1075142 mem = 536879116 peak = 959643648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277464, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 376, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (393.44, 169.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670898 sequences (100000246 bp) in 2.333 CPU sec, 3.021 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277533, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 380, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (396.70, 169.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671154 reads in 183.399 CPU sec, 6.039 real sec
[M::process] read 671014 sequences (100000238 bp) in 1.949 CPU sec, 2.008 real sec...
[M::mem_process_seqs] Processed 670878 reads in 116.906 CPU sec, 3.784 real sec
sorting block 28, #reads = 1074658 mem = 536880156 peak = 959643648
sorting block 29, #reads = 1075169 mem = 536879865 peak = 959643648
[M::process] read 670652 sequences (100000273 bp) in 2.794 CPU sec, 3.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278005, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (395.31, 168.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671014 reads in 100.645 CPU sec, 3.271 real sec
[M::process] read 670516 sequences (100000268 bp) in 2.081 CPU sec, 2.101 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277738, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 379, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (395.76, 169.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278110, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 385, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (401.87, 172.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670898 reads in 280.847 CPU sec, 9.127 real sec
[M::mem_process_seqs] Processed 670652 reads in 132.328 CPU sec, 4.318 real sec
[M::process] read 670628 sequences (100000249 bp) in 2.386 CPU sec, 2.511 real sec...
sorting block 30, #reads = 1075379 mem = 536879638 peak = 959643648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 278112, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (319, 319, 1452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3718)
[M::mem_pestat] mean and std.dev: (682.08, 561.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4851)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 389, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (404.48, 173.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 670446 sequences (100000239 bp) in 2.651 CPU sec, 3.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278113, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 388, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (404.28, 173.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670516 reads in 177.916 CPU sec, 5.790 real sec
sorting block 31, #reads = 1075361 mem = 536880123 peak = 959643648
[M::mem_process_seqs] Processed 670628 reads in 115.472 CPU sec, 3.685 real sec
[M::process] read 670480 sequences (100000078 bp) in 2.333 CPU sec, 2.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277956, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 390, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (405.99, 174.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 32, #reads = 1074497 mem = 536880500 peak = 959643648
[M::process] read 670436 sequences (100000249 bp) in 2.367 CPU sec, 2.993 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278204, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 390, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (406.57, 174.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670446 reads in 174.457 CPU sec, 5.749 real sec
[M::mem_process_seqs] Processed 670480 reads in 112.554 CPU sec, 3.738 real sec
[M::process] read 670248 sequences (100000248 bp) in 1.941 CPU sec, 2.112 real sec...
sorting block 33, #reads = 1075206 mem = 536880770 peak = 959643648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278839, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 396, 533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (412.15, 178.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1313)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672980 sequences (100000118 bp) in 2.572 CPU sec, 2.971 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278669, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 401, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067)
[M::mem_pestat] mean and std.dev: (416.63, 180.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1331)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670436 reads in 178.530 CPU sec, 5.795 real sec
[M::mem_process_seqs] Processed 670248 reads in 115.923 CPU sec, 3.700 real sec
[M::process] read 672836 sequences (100000066 bp) in 2.360 CPU sec, 2.497 real sec...
sorting block 34, #reads = 1075340 mem = 536880295 peak = 959643648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278609, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 363, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (379.49, 165.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672910 sequences (100000095 bp) in 2.889 CPU sec, 3.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278603, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 367, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (380.87, 163.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672980 reads in 184.259 CPU sec, 5.941 real sec
[M::mem_process_seqs] Processed 672836 reads in 116.921 CPU sec, 3.723 real sec
[M::process] read 672442 sequences (100000157 bp) in 2.343 CPU sec, 2.591 real sec...
sorting block 35, #reads = 1073631 mem = 536879483 peak = 959643648
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278586, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 363, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (378.07, 162.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672286 sequences (100000246 bp) in 2.504 CPU sec, 3.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278609, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 366, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (380.02, 161.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672910 reads in 187.520 CPU sec, 6.129 real sec
sorting block 36, #reads = 1075272 mem = 536877449 peak = 973316096
[M::mem_process_seqs] Processed 672442 reads in 118.165 CPU sec, 3.824 real sec
[M::process] read 672128 sequences (100000100 bp) in 2.320 CPU sec, 2.550 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278406, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 368, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (381.65, 161.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (5662, 5757, 5757)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (5472, 5947)
[M::mem_pestat] mean and std.dev: (5718.89, 56.16)
[M::mem_pestat] low and high boundaries for proper pairs: (5377, 6042)
[M::mem_pestat] skip orientation RR
sorting block 37, #reads = 1075503 mem = 536878570 peak = 1033519104
[M::process] read 671672 sequences (100000144 bp) in 2.673 CPU sec, 3.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278813, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 373, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (385.56, 163.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672286 reads in 185.210 CPU sec, 6.082 real sec
[M::mem_process_seqs] Processed 672128 reads in 118.283 CPU sec, 3.859 real sec
[M::process] read 671502 sequences (100000012 bp) in 2.090 CPU sec, 2.238 real sec...
sorting block 38, #reads = 1075843 mem = 536878434 peak = 1033519104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278109, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 377, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (389.29, 164.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671524 sequences (100000059 bp) in 2.345 CPU sec, 2.984 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277538, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 376, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (390.13, 164.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671672 reads in 184.302 CPU sec, 6.068 real sec
[M::mem_process_seqs] Processed 671502 reads in 117.359 CPU sec, 3.959 real sec
[M::process] read 671272 sequences (100000159 bp) in 2.027 CPU sec, 2.323 real sec...
sorting block 39, #reads = 1075758 mem = 536880850 peak = 1033519104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 277830, 0, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (576, 2276, 4996)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13836)
[M::mem_pestat] mean and std.dev: (2627.60, 2049.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18256)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 377, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (390.63, 164.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 671268 sequences (100000015 bp) in 2.544 CPU sec, 3.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278314, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 380, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (393.82, 166.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671524 reads in 184.840 CPU sec, 6.085 real sec
[M::process] read 670926 sequences (100000047 bp) in 2.033 CPU sec, 2.079 real sec...
[M::mem_process_seqs] Processed 671272 reads in 118.497 CPU sec, 3.866 real sec
sorting block 40, #reads = 1075261 mem = 536878727 peak = 1033519104
[M::process] read 670892 sequences (100000023 bp) in 2.375 CPU sec, 3.173 real sec...
sorting block 41, #reads = 1074995 mem = 536880937 peak = 1033519104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 277221, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (396.76, 167.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670926 reads in 105.126 CPU sec, 3.375 real sec
[M::process] read 670926 sequences (100000134 bp) in 2.086 CPU sec, 2.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277396, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (393.54, 165.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277545, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 385, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (398.53, 167.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671268 reads in 291.120 CPU sec, 9.434 real sec
[M::mem_process_seqs] Processed 670892 reads in 138.910 CPU sec, 4.432 real sec
[M::process] read 671378 sequences (100000237 bp) in 2.561 CPU sec, 2.689 real sec...
sorting block 42, #reads = 1074673 mem = 536880206 peak = 1033519104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277497, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (395.69, 167.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670986 sequences (100000230 bp) in 2.561 CPU sec, 3.105 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277691, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 377, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (392.67, 167.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670926 reads in 187.179 CPU sec, 6.070 real sec
sorting block 43, #reads = 1075308 mem = 536877783 peak = 1033519104
[M::mem_process_seqs] Processed 671378 reads in 120.359 CPU sec, 3.870 real sec
[M::process] read 670996 sequences (100000151 bp) in 2.250 CPU sec, 2.402 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277997, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 380, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (395.32, 167.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 44, #reads = 1075494 mem = 536880292 peak = 1033519104
[M::process] read 670964 sequences (100000141 bp) in 3.293 CPU sec, 3.605 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278167, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 377, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (392.93, 166.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670986 reads in 189.611 CPU sec, 6.103 real sec
[M::mem_process_seqs] Processed 670996 reads in 121.743 CPU sec, 3.872 real sec
[M::process] read 671130 sequences (100000115 bp) in 1.998 CPU sec, 2.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 276795, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (393.47, 167.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1074908 mem = 536884819 peak = 1033519104
[M::process] read 671064 sequences (100000208 bp) in 2.747 CPU sec, 3.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277829, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (393.86, 167.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670964 reads in 190.904 CPU sec, 6.239 real sec
[M::mem_process_seqs] Processed 671130 reads in 123.521 CPU sec, 3.965 real sec
[M::process] read 670950 sequences (100000136 bp) in 2.364 CPU sec, 2.459 real sec...
sorting block 46, #reads = 1074756 mem = 536881078 peak = 1033519104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277638, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 379, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (395.13, 168.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (5296, 5317, 5979)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (3930, 7345)
[M::mem_pestat] mean and std.dev: (5606.56, 333.21)
[M::mem_pestat] low and high boundaries for proper pairs: (3247, 8028)
[M::mem_pestat] skip orientation RR
[M::process] read 670758 sequences (100000090 bp) in 2.452 CPU sec, 2.837 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277982, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (391.50, 165.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671064 reads in 190.286 CPU sec, 6.159 real sec
[M::process] read 670728 sequences (100000269 bp) in 1.874 CPU sec, 1.967 real sec...
[M::mem_process_seqs] Processed 670950 reads in 124.049 CPU sec, 3.974 real sec
sorting block 47, #reads = 1073858 mem = 536877854 peak = 1033519104
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278089, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 379, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (395.78, 168.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670816 sequences (100000151 bp) in 2.425 CPU sec, 3.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277858, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 385, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (400.03, 170.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670688 sequences (100000252 bp) in 1.990 CPU sec, 2.011 real sec...
[M::mem_process_seqs] Processed 670758 reads in 188.392 CPU sec, 6.072 real sec
sorting block 48, #reads = 1074284 mem = 536879569 peak = 1041088512
[M::mem_process_seqs] Processed 670728 reads in 120.821 CPU sec, 3.853 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278023, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 386, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (401.38, 171.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1074059 mem = 536884301 peak = 1043550208
[M::process] read 670806 sequences (100000227 bp) in 2.886 CPU sec, 3.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277901, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 388, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (403.89, 173.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670574 sequences (100000119 bp) in 1.921 CPU sec, 1.929 real sec...
[M::mem_process_seqs] Processed 670816 reads in 182.695 CPU sec, 5.876 real sec
[M::mem_process_seqs] Processed 670688 reads in 117.974 CPU sec, 3.772 real sec
sorting block 50, #reads = 1072671 mem = 536878348 peak = 1043550208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278135, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 387, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (404.33, 175.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671080 sequences (100000091 bp) in 2.616 CPU sec, 3.087 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278346, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 390, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (406.66, 176.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670806 reads in 180.124 CPU sec, 5.843 real sec
[M::process] read 672794 sequences (100000125 bp) in 1.951 CPU sec, 1.994 real sec...
[M::mem_process_seqs] Processed 670574 reads in 118.465 CPU sec, 3.816 real sec
sorting block 51, #reads = 1074385 mem = 536878885 peak = 1043550208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278352, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 383, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (401.31, 175.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672340 sequences (100000202 bp) in 2.743 CPU sec, 3.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277572, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 364, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (379.60, 163.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671080 reads in 183.567 CPU sec, 5.949 real sec
[M::process] read 672382 sequences (100000248 bp) in 2.246 CPU sec, 2.330 real sec...
[M::mem_process_seqs] Processed 672794 reads in 119.953 CPU sec, 3.929 real sec
sorting block 52, #reads = 1073330 mem = 536878979 peak = 1043550208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277298, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 368, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (383.80, 165.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672088 sequences (100000069 bp) in 2.368 CPU sec, 2.818 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278170, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 369, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (383.91, 164.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672340 reads in 182.258 CPU sec, 5.914 real sec
sorting block 53, #reads = 1074584 mem = 536878968 peak = 1043550208
[M::process] read 672036 sequences (100000222 bp) in 2.116 CPU sec, 2.148 real sec...
[M::mem_process_seqs] Processed 672382 reads in 116.372 CPU sec, 3.713 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277843, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 371, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (386.27, 165.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 54, #reads = 1076102 mem = 536880844 peak = 1076752384
[M::process] read 671892 sequences (100000245 bp) in 2.343 CPU sec, 2.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277768, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 373, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (387.47, 165.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672088 reads in 185.425 CPU sec, 6.035 real sec
[M::process] read 671810 sequences (100000031 bp) in 1.991 CPU sec, 2.135 real sec...
[M::mem_process_seqs] Processed 672036 reads in 118.501 CPU sec, 3.815 real sec
sorting block 55, #reads = 1076551 mem = 536878648 peak = 1076752384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277470, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 374, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (388.29, 165.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671448 sequences (100000078 bp) in 2.374 CPU sec, 3.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277489, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (391.32, 166.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671892 reads in 187.347 CPU sec, 6.088 real sec
[M::process] read 671376 sequences (100000034 bp) in 2.069 CPU sec, 2.201 real sec...
[M::mem_process_seqs] Processed 671810 reads in 119.746 CPU sec, 3.872 real sec
sorting block 56, #reads = 1076233 mem = 536878277 peak = 1076752384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 276980, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (393.92, 166.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671260 sequences (100000024 bp) in 2.820 CPU sec, 3.176 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277537, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 379, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (392.90, 165.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671124 sequences (100000251 bp) in 1.945 CPU sec, 1.946 real sec...
[M::mem_process_seqs] Processed 671448 reads in 190.171 CPU sec, 6.124 real sec
[M::mem_process_seqs] Processed 671376 reads in 121.846 CPU sec, 3.909 real sec
sorting block 57, #reads = 1075725 mem = 536878942 peak = 1076752384
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278081, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (393.85, 165.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 359, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (323.90, 75.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RR
[M::process] read 671142 sequences (100000068 bp) in 2.468 CPU sec, 3.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 276836, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (394.74, 166.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671260 reads in 189.753 CPU sec, 6.160 real sec
sorting block 58, #reads = 1075527 mem = 536878923 peak = 1076752384
[M::process] read 671300 sequences (100000250 bp) in 2.048 CPU sec, 2.165 real sec...
[M::mem_process_seqs] Processed 671124 reads in 122.093 CPU sec, 3.917 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277397, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (394.50, 165.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 59, #reads = 1075198 mem = 536880789 peak = 1079705600
[M::process] read 671508 sequences (100000134 bp) in 2.893 CPU sec, 3.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277605, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 383, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (396.28, 167.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671940 sequences (100000051 bp) in 1.968 CPU sec, 2.010 real sec...
[M::mem_process_seqs] Processed 671142 reads in 190.575 CPU sec, 6.130 real sec
[M::mem_process_seqs] Processed 671300 reads in 121.320 CPU sec, 3.885 real sec
sorting block 60, #reads = 1074910 mem = 536880844 peak = 1079705600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277745, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 381, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (395.16, 169.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671524 sequences (100000234 bp) in 2.493 CPU sec, 2.928 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277933, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 367, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (384.46, 166.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671544 sequences (100000121 bp) in 1.962 CPU sec, 1.966 real sec...
[M::mem_process_seqs] Processed 671508 reads in 189.265 CPU sec, 6.138 real sec
[M::mem_process_seqs] Processed 671940 reads in 121.566 CPU sec, 3.928 real sec
sorting block 61, #reads = 1074758 mem = 536880943 peak = 1079705600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 277488, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 373, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (388.32, 164.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671432 sequences (100000220 bp) in 2.472 CPU sec, 2.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277687, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 372, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (386.40, 163.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671382 sequences (100000216 bp) in 2.020 CPU sec, 2.172 real sec...
[M::mem_process_seqs] Processed 671524 reads in 189.899 CPU sec, 6.144 real sec
[M::mem_process_seqs] Processed 671544 reads in 122.281 CPU sec, 3.916 real sec
sorting block 62, #reads = 1073791 mem = 536879304 peak = 1079705600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 276516, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 370, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (385.65, 162.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671422 sequences (100000035 bp) in 2.460 CPU sec, 2.872 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277514, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 369, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (384.82, 162.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671234 sequences (100000153 bp) in 2.028 CPU sec, 2.039 real sec...
[M::mem_process_seqs] Processed 671432 reads in 190.816 CPU sec, 6.204 real sec
sorting block 63, #reads = 1073975 mem = 536880777 peak = 1079705600
[M::mem_process_seqs] Processed 671382 reads in 119.302 CPU sec, 3.853 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277590, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 369, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (384.47, 162.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 64, #reads = 1074581 mem = 536879235 peak = 1079705600
[M::process] read 671242 sequences (100000145 bp) in 2.854 CPU sec, 3.456 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278327, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 367, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (383.69, 161.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671422 reads in 186.789 CPU sec, 6.028 real sec
[M::process] read 670962 sequences (100000180 bp) in 1.902 CPU sec, 1.911 real sec...
[M::mem_process_seqs] Processed 671234 reads in 118.042 CPU sec, 3.791 real sec
sorting block 65, #reads = 1073891 mem = 536879419 peak = 1079705600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277790, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 366, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (382.05, 160.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670960 sequences (100000177 bp) in 2.836 CPU sec, 3.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 277835, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 373, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (388.48, 163.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671242 reads in 182.500 CPU sec, 5.860 real sec
[M::process] read 670862 sequences (100000117 bp) in 1.891 CPU sec, 1.908 real sec...
[M::mem_process_seqs] Processed 670962 reads in 116.710 CPU sec, 3.719 real sec
sorting block 66, #reads = 1073558 mem = 536880547 peak = 1079705600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277921, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 378, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (393.31, 165.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670822 sequences (100000040 bp) in 2.608 CPU sec, 3.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 277592, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 383, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (398.04, 169.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670716 sequences (100000235 bp) in 1.902 CPU sec, 1.905 real sec...
[M::mem_process_seqs] Processed 670960 reads in 181.761 CPU sec, 5.855 real sec
[M::mem_process_seqs] Processed 670862 reads in 115.123 CPU sec, 3.652 real sec
sorting block 67, #reads = 1074107 mem = 536879694 peak = 1079705600
sorting block 68, #reads = 1074335 mem = 536878628 peak = 1079705600
[M::process] read 672914 sequences (100000224 bp) in 2.791 CPU sec, 3.059 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277569, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 389, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (404.36, 172.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670716 reads in 98.044 CPU sec, 3.134 real sec
[M::process] read 674392 sequences (100000080 bp) in 1.915 CPU sec, 2.273 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 277928, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 383, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (398.90, 170.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279481, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 365, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (385.32, 171.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670822 reads in 281.499 CPU sec, 9.056 real sec
[M::mem_process_seqs] Processed 672914 reads in 139.103 CPU sec, 4.486 real sec
[M::process] read 673572 sequences (100000033 bp) in 2.596 CPU sec, 2.675 real sec...
sorting block 69, #reads = 1073490 mem = 536879618 peak = 1079705600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279480, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 346, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (369.16, 167.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672662 sequences (100000021 bp) in 2.750 CPU sec, 3.047 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279439, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 358, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (377.12, 168.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672372 sequences (100000002 bp) in 1.957 CPU sec, 1.965 real sec...
[M::mem_process_seqs] Processed 674392 reads in 190.596 CPU sec, 6.090 real sec
sorting block 70, #reads = 1071683 mem = 536881893 peak = 1079705600
[M::mem_process_seqs] Processed 673572 reads in 122.888 CPU sec, 3.894 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278926, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 370, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (388.42, 173.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 71, #reads = 1073408 mem = 536879564 peak = 1079705600
[M::process] read 671960 sequences (100000031 bp) in 2.658 CPU sec, 2.949 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278534, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 373, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (391.11, 173.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672028 sequences (100000088 bp) in 1.880 CPU sec, 1.914 real sec...
[M::mem_process_seqs] Processed 672662 reads in 184.363 CPU sec, 5.946 real sec
[M::mem_process_seqs] Processed 672372 reads in 118.366 CPU sec, 3.787 real sec
sorting block 72, #reads = 1075229 mem = 536878621 peak = 1079705600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278984, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 379, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (395.33, 173.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671790 sequences (100000157 bp) in 2.716 CPU sec, 3.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278569, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 378, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (393.50, 172.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671402 sequences (100000245 bp) in 1.958 CPU sec, 2.020 real sec...
[M::mem_process_seqs] Processed 671960 reads in 185.863 CPU sec, 5.963 real sec
[M::mem_process_seqs] Processed 672028 reads in 121.539 CPU sec, 3.876 real sec
sorting block 73, #reads = 1073554 mem = 536878247 peak = 1079705600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278957, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 381, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (396.40, 172.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671552 sequences (100000082 bp) in 3.021 CPU sec, 3.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278098, 12, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 383, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (398.03, 171.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (759, 2049, 2049)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4629)
[M::mem_pestat] mean and std.dev: (1719.73, 1104.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6136)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 671790 reads in 185.399 CPU sec, 5.940 real sec
[M::process] read 671372 sequences (100000018 bp) in 2.019 CPU sec, 2.027 real sec...
[M::mem_process_seqs] Processed 671402 reads in 120.878 CPU sec, 3.856 real sec
sorting block 74, #reads = 1073192 mem = 536879579 peak = 1079705600
[M::process] read 671496 sequences (100000113 bp) in 3.012 CPU sec, 3.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278277, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (397.99, 170.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671314 sequences (100000182 bp) in 2.038 CPU sec, 2.041 real sec...
[M::mem_process_seqs] Processed 671372 reads in 112.439 CPU sec, 3.552 real sec
sorting block 75, #reads = 1073085 mem = 536879044 peak = 1079705600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278835, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 385, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (399.22, 172.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (341, 342, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (11, 836)
[M::mem_pestat] mean and std.dev: (349.25, 98.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278228, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (397.94, 171.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (434, 434, 5956)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17000)
[M::mem_pestat] mean and std.dev: (1913.27, 2478.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 22522)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 671552 reads in 298.635 CPU sec, 9.543 real sec
[M::process] read 671322 sequences (100000301 bp) in 1.970 CPU sec, 1.986 real sec...
[M::mem_process_seqs] Processed 671496 reads in 143.464 CPU sec, 4.600 real sec
sorting block 76, #reads = 1072511 mem = 536883765 peak = 1079705600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278584, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 383, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (397.42, 170.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671298 sequences (100000188 bp) in 3.005 CPU sec, 3.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278370, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (398.25, 170.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671312 sequences (100000198 bp) in 1.879 CPU sec, 1.883 real sec...
[M::mem_process_seqs] Processed 671314 reads in 195.938 CPU sec, 6.272 real sec
sorting block 77, #reads = 1071344 mem = 536878018 peak = 1079705600
[M::mem_process_seqs] Processed 671322 reads in 124.839 CPU sec, 3.972 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278947, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 382, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (397.20, 170.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 78, #reads = 1072389 mem = 536877773 peak = 1079705600
[M::process] read 671168 sequences (100000040 bp) in 2.877 CPU sec, 3.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278189, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 380, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (395.61, 169.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671006 sequences (100000077 bp) in 1.993 CPU sec, 2.047 real sec...
[M::mem_process_seqs] Processed 671298 reads in 194.134 CPU sec, 6.200 real sec
[M::mem_process_seqs] Processed 671312 reads in 124.098 CPU sec, 3.934 real sec
sorting block 79, #reads = 1072504 mem = 536883436 peak = 1079705600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279143, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 380, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (395.00, 168.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671102 sequences (100000100 bp) in 2.995 CPU sec, 3.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278147, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 381, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (395.60, 168.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671092 sequences (100000068 bp) in 2.021 CPU sec, 2.023 real sec...
[M::mem_process_seqs] Processed 671168 reads in 194.969 CPU sec, 6.262 real sec
[M::mem_process_seqs] Processed 671006 reads in 125.231 CPU sec, 3.994 real sec
sorting block 80, #reads = 1071040 mem = 536877021 peak = 1079705600
[M::process] read 671084 sequences (100000223 bp) in 2.966 CPU sec, 3.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278575, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 379, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (394.17, 167.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670998 sequences (100000154 bp) in 1.831 CPU sec, 1.854 real sec...
[M::mem_process_seqs] Processed 671092 reads in 114.209 CPU sec, 3.615 real sec
sorting block 81, #reads = 1071025 mem = 536882452 peak = 1079705600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278572, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 381, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (396.44, 169.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278101, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 380, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (395.08, 168.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 671102 reads in 301.258 CPU sec, 9.662 real sec
[M::process] read 671122 sequences (100000267 bp) in 2.096 CPU sec, 2.291 real sec...
[M::mem_process_seqs] Processed 671084 reads in 143.540 CPU sec, 4.622 real sec
sorting block 82, #reads = 1071025 mem = 536878760 peak = 1079705600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278537, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (396.08, 168.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670982 sequences (100000291 bp) in 2.636 CPU sec, 3.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278924, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 382, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (397.14, 169.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670840 sequences (100000240 bp) in 1.913 CPU sec, 1.915 real sec...
[M::mem_process_seqs] Processed 670998 reads in 189.646 CPU sec, 6.123 real sec
[M::mem_process_seqs] Processed 671122 reads in 121.344 CPU sec, 3.902 real sec
sorting block 83, #reads = 1071299 mem = 536878417 peak = 1079705600
[M::process] read 670678 sequences (100000019 bp) in 2.506 CPU sec, 2.801 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278780, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 386, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (402.48, 173.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672480 sequences (100000042 bp) in 1.976 CPU sec, 1.983 real sec...
[M::mem_process_seqs] Processed 670840 reads in 106.998 CPU sec, 3.412 real sec
sorting block 84, #reads = 1071668 mem = 536876343 peak = 1079705600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278851, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 384, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (399.08, 171.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279153, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 390, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (405.95, 174.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670982 reads in 292.877 CPU sec, 9.389 real sec
[M::process] read 676126 sequences (100000122 bp) in 2.166 CPU sec, 2.183 real sec...
[M::mem_process_seqs] Processed 670678 reads in 142.989 CPU sec, 4.571 real sec
sorting block 85, #reads = 1072068 mem = 536877158 peak = 1079705600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279634, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 370, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (389.03, 173.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675888 sequences (100000218 bp) in 2.779 CPU sec, 3.236 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281054, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 328, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (350.34, 158.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674966 sequences (100000279 bp) in 2.033 CPU sec, 2.041 real sec...
[M::mem_process_seqs] Processed 672480 reads in 200.109 CPU sec, 6.431 real sec
[M::mem_process_seqs] Processed 676126 reads in 131.317 CPU sec, 4.173 real sec
sorting block 86, #reads = 1071616 mem = 536879214 peak = 1079705600
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280626, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (351.51, 156.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674250 sequences (100000203 bp) in 2.672 CPU sec, 3.189 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280103, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 339, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (356.95, 156.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673772 sequences (100000279 bp) in 2.034 CPU sec, 2.039 real sec...
[M::mem_process_seqs] Processed 675888 reads in 202.693 CPU sec, 6.530 real sec
sorting block 87, #reads = 1070525 mem = 536879604 peak = 1079705600
[M::mem_process_seqs] Processed 674966 reads in 130.783 CPU sec, 4.151 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280110, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 347, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (363.86, 158.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 88, #reads = 1067886 mem = 536882502 peak = 1093890048
[M::process] read 673526 sequences (100000138 bp) in 2.546 CPU sec, 2.894 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279688, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 354, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (369.71, 161.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673160 sequences (100000049 bp) in 2.032 CPU sec, 2.042 real sec...
[M::mem_process_seqs] Processed 674250 reads in 194.401 CPU sec, 6.254 real sec
[M::mem_process_seqs] Processed 673772 reads in 127.069 CPU sec, 4.031 real sec
sorting block 89, #reads = 1069798 mem = 536878304 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279875, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 358, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (374.14, 162.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673120 sequences (100000176 bp) in 2.633 CPU sec, 3.110 real sec...
[M::process] read 672954 sequences (100000260 bp) in 2.037 CPU sec, 2.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280267, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 362, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (377.27, 163.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673526 reads in 196.565 CPU sec, 6.333 real sec
[M::mem_process_seqs] Processed 673160 reads in 128.413 CPU sec, 4.093 real sec
sorting block 90, #reads = 1069853 mem = 536879863 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279364, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 363, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (377.44, 163.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672488 sequences (100000127 bp) in 2.734 CPU sec, 2.994 real sec...
[M::process] read 672190 sequences (100000101 bp) in 1.821 CPU sec, 1.837 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279220, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 364, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (378.17, 162.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673120 reads in 194.585 CPU sec, 6.235 real sec
[M::mem_process_seqs] Processed 672954 reads in 127.432 CPU sec, 4.063 real sec
sorting block 91, #reads = 1070324 mem = 536880135 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279625, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 369, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (383.61, 163.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672006 sequences (100000065 bp) in 2.737 CPU sec, 3.030 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278825, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 376, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (388.89, 165.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671684 sequences (100000064 bp) in 2.062 CPU sec, 2.067 real sec...
[M::mem_process_seqs] Processed 672488 reads in 196.579 CPU sec, 6.295 real sec
sorting block 92, #reads = 1069935 mem = 536879617 peak = 1093890048
[M::mem_process_seqs] Processed 672190 reads in 130.569 CPU sec, 4.141 real sec
sorting block 93, #reads = 1069972 mem = 536878623 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279544, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 375, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (389.06, 165.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671480 sequences (100000224 bp) in 2.923 CPU sec, 3.328 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278907, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 376, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (391.12, 167.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671668 sequences (100000106 bp) in 2.019 CPU sec, 2.025 real sec...
[M::mem_process_seqs] Processed 672006 reads in 195.518 CPU sec, 6.244 real sec
[M::mem_process_seqs] Processed 671684 reads in 128.272 CPU sec, 4.064 real sec
sorting block 94, #reads = 1070417 mem = 536879488 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279088, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 377, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (391.93, 167.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671398 sequences (100000032 bp) in 2.658 CPU sec, 3.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278890, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 378, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (393.08, 168.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671138 sequences (100000167 bp) in 2.118 CPU sec, 2.129 real sec...
[M::mem_process_seqs] Processed 671480 reads in 197.094 CPU sec, 6.322 real sec
[M::mem_process_seqs] Processed 671668 reads in 129.900 CPU sec, 4.157 real sec
sorting block 95, #reads = 1070912 mem = 536881253 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278653, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 381, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (395.89, 170.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671152 sequences (100000038 bp) in 2.767 CPU sec, 3.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278265, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 382, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (397.40, 171.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671230 sequences (100000072 bp) in 2.015 CPU sec, 2.024 real sec...
[M::mem_process_seqs] Processed 671398 reads in 195.214 CPU sec, 6.251 real sec
sorting block 96, #reads = 1071151 mem = 536878883 peak = 1093890048
[M::mem_process_seqs] Processed 671138 reads in 127.419 CPU sec, 4.055 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278719, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 380, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (395.54, 169.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670810 sequences (100000167 bp) in 2.836 CPU sec, 3.236 real sec...
sorting block 97, #reads = 1071591 mem = 536879487 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278380, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 380, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (395.05, 169.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670940 sequences (100000211 bp) in 2.001 CPU sec, 2.003 real sec...
[M::mem_process_seqs] Processed 671152 reads in 191.745 CPU sec, 6.157 real sec
[M::mem_process_seqs] Processed 671230 reads in 126.294 CPU sec, 4.024 real sec
sorting block 98, #reads = 1071122 mem = 536879340 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 277981, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 385, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (401.09, 172.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670836 sequences (100000042 bp) in 2.873 CPU sec, 3.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278584, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 384, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (400.62, 172.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670948 sequences (100000036 bp) in 2.019 CPU sec, 2.020 real sec...
[M::mem_process_seqs] Processed 670810 reads in 187.849 CPU sec, 6.011 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278767, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 387, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (403.03, 173.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671644 sequences (100000147 bp) in 2.343 CPU sec, 2.388 real sec...
[M::mem_process_seqs] Processed 670836 reads in 106.508 CPU sec, 3.454 real sec
sorting block 99, #reads = 1071695 mem = 536884507 peak = 1093890048
[M::mem_process_seqs] Processed 670940 reads in 268.182 CPU sec, 8.612 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278523, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 388, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049)
[M::mem_pestat] mean and std.dev: (404.77, 176.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 100, #reads = 1072835 mem = 536880919 peak = 1093890048
[M::process] read 675576 sequences (100000207 bp) in 2.732 CPU sec, 3.033 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279450, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 380, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (399.11, 176.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1304)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670948 reads in 219.762 CPU sec, 7.058 real sec
[M::process] read 674374 sequences (100000237 bp) in 1.895 CPU sec, 1.900 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279388, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 336, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (356.18, 158.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673872 sequences (100000111 bp) in 2.145 CPU sec, 2.166 real sec...
[M::mem_process_seqs] Processed 675576 reads in 107.666 CPU sec, 3.424 real sec
sorting block 101, #reads = 1071980 mem = 536879209 peak = 1093890048
[M::mem_process_seqs] Processed 671644 reads in 286.853 CPU sec, 9.173 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279469, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 348, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (366.06, 161.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 102, #reads = 1068746 mem = 536884671 peak = 1093890048
[M::process] read 673418 sequences (100000133 bp) in 2.723 CPU sec, 3.023 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278893, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 353, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (370.06, 162.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674374 reads in 213.103 CPU sec, 6.853 real sec
[M::process] read 673240 sequences (100000215 bp) in 1.971 CPU sec, 2.037 real sec...
[M::mem_process_seqs] Processed 673872 reads in 124.573 CPU sec, 4.001 real sec
sorting block 103, #reads = 1074222 mem = 536879377 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278473, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 355, 484)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (371.79, 161.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672810 sequences (100000061 bp) in 2.636 CPU sec, 2.964 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278380, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 360, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (375.19, 162.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672482 sequences (100000010 bp) in 1.985 CPU sec, 1.985 real sec...
[M::mem_process_seqs] Processed 673418 reads in 192.797 CPU sec, 6.209 real sec
[M::mem_process_seqs] Processed 673240 reads in 121.576 CPU sec, 3.883 real sec
sorting block 104, #reads = 1074367 mem = 536878594 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278324, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 362, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (377.11, 162.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672232 sequences (100000054 bp) in 2.980 CPU sec, 3.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278617, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 364, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (378.85, 161.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672530 sequences (100000214 bp) in 2.019 CPU sec, 2.019 real sec...
[M::mem_process_seqs] Processed 672810 reads in 192.585 CPU sec, 6.157 real sec
[M::mem_process_seqs] Processed 672482 reads in 121.861 CPU sec, 3.870 real sec
sorting block 105, #reads = 1074378 mem = 536879489 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278269, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 368, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (381.94, 162.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672404 sequences (100000064 bp) in 2.846 CPU sec, 3.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278822, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 367, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (380.96, 162.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672470 sequences (100000177 bp) in 2.098 CPU sec, 2.107 real sec...
[M::mem_process_seqs] Processed 672232 reads in 193.688 CPU sec, 6.204 real sec
sorting block 106, #reads = 1072660 mem = 536878107 peak = 1093890048
[M::mem_process_seqs] Processed 672530 reads in 121.706 CPU sec, 3.875 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278371, 0, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 368, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (381.40, 162.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (920, 920, 920)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (920, 920)
[M::mem_pestat] mean and std.dev: (920.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (920, 920)
[M::mem_pestat] skip orientation RR
sorting block 107, #reads = 1073188 mem = 536878744 peak = 1093890048
[M::process] read 672104 sequences (100000290 bp) in 2.889 CPU sec, 3.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278299, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 367, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (380.60, 161.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672520 sequences (100000116 bp) in 1.981 CPU sec, 2.135 real sec...
[M::mem_process_seqs] Processed 672404 reads in 188.433 CPU sec, 6.042 real sec
[M::mem_process_seqs] Processed 672470 reads in 120.721 CPU sec, 3.868 real sec
sorting block 108, #reads = 1073289 mem = 536880243 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 277856, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 369, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (382.85, 161.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672104 sequences (100000004 bp) in 2.950 CPU sec, 3.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279121, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 362, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (377.16, 161.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671792 sequences (100000143 bp) in 2.077 CPU sec, 2.084 real sec...
[M::mem_process_seqs] Processed 672104 reads in 190.925 CPU sec, 6.120 real sec
[M::mem_process_seqs] Processed 672520 reads in 122.931 CPU sec, 3.919 real sec
sorting block 109, #reads = 1072702 mem = 536877694 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278595, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 369, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (383.26, 163.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671840 sequences (100000038 bp) in 2.654 CPU sec, 3.011 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278677, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 375, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (388.08, 164.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671488 sequences (100000132 bp) in 2.084 CPU sec, 2.086 real sec...
[M::mem_process_seqs] Processed 672104 reads in 196.926 CPU sec, 6.328 real sec
[M::mem_process_seqs] Processed 671792 reads in 129.276 CPU sec, 4.135 real sec
sorting block 110, #reads = 1072101 mem = 536878199 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278516, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 373, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (387.00, 163.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671374 sequences (100000235 bp) in 2.629 CPU sec, 3.189 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278291, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (391.25, 165.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671902 sequences (100000231 bp) in 1.997 CPU sec, 2.000 real sec...
[M::mem_process_seqs] Processed 671840 reads in 197.291 CPU sec, 6.363 real sec
[M::mem_process_seqs] Processed 671488 reads in 128.826 CPU sec, 4.114 real sec
sorting block 111, #reads = 1071842 mem = 536879676 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278522, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 377, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (392.21, 166.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 112, #reads = 1071581 mem = 536889039 peak = 1093890048
[M::process] read 671484 sequences (100000012 bp) in 2.541 CPU sec, 3.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278731, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 371, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (386.37, 165.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671268 sequences (100000030 bp) in 2.092 CPU sec, 2.227 real sec...
[M::mem_process_seqs] Processed 671374 reads in 199.982 CPU sec, 6.503 real sec
[M::mem_process_seqs] Processed 671902 reads in 128.074 CPU sec, 4.152 real sec
sorting block 113, #reads = 1070771 mem = 536878807 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278093, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 373, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (387.60, 164.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671332 sequences (100000134 bp) in 2.728 CPU sec, 3.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278322, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 378, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (393.10, 167.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671426 sequences (100000097 bp) in 2.045 CPU sec, 2.070 real sec...
[M::mem_process_seqs] Processed 671484 reads in 201.191 CPU sec, 6.497 real sec
[M::mem_process_seqs] Processed 671268 reads in 129.792 CPU sec, 4.181 real sec
sorting block 114, #reads = 1070262 mem = 536878722 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278646, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 378, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (393.57, 168.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671148 sequences (100000006 bp) in 2.529 CPU sec, 3.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278469, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 378, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (393.21, 169.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671568 sequences (100000106 bp) in 2.039 CPU sec, 2.053 real sec...
[M::mem_process_seqs] Processed 671332 reads in 196.918 CPU sec, 6.362 real sec
[M::mem_process_seqs] Processed 671426 reads in 129.183 CPU sec, 4.134 real sec
sorting block 115, #reads = 1069875 mem = 536878173 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 278509, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 379, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (395.82, 170.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675390 sequences (100000095 bp) in 2.652 CPU sec, 3.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279230, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 379, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (396.47, 173.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676806 sequences (100000153 bp) in 2.070 CPU sec, 2.078 real sec...
[M::mem_process_seqs] Processed 671148 reads in 204.467 CPU sec, 6.579 real sec
sorting block 116, #reads = 1068489 mem = 536879375 peak = 1093890048
[M::mem_process_seqs] Processed 671568 reads in 137.625 CPU sec, 4.375 real sec
sorting block 117, #reads = 1066948 mem = 536880357 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280142, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 338, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (360.16, 163.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675814 sequences (100000134 bp) in 2.702 CPU sec, 3.188 real sec...
[M::process] read 675188 sequences (100000105 bp) in 2.038 CPU sec, 2.038 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280577, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (343.99, 153.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675390 reads in 212.527 CPU sec, 6.862 real sec
[M::mem_process_seqs] Processed 676806 reads in 133.909 CPU sec, 4.301 real sec
sorting block 118, #reads = 1063387 mem = 536878252 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280268, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (350.21, 152.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674490 sequences (100000010 bp) in 2.665 CPU sec, 3.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280675, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (234, 338, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (353.75, 152.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674002 sequences (100000079 bp) in 2.082 CPU sec, 2.143 real sec...
[M::mem_process_seqs] Processed 675814 reads in 205.469 CPU sec, 6.632 real sec
[M::mem_process_seqs] Processed 675188 reads in 132.299 CPU sec, 4.241 real sec
sorting block 119, #reads = 1068349 mem = 536878860 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280529, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 345, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (359.87, 154.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673572 sequences (100000232 bp) in 2.781 CPU sec, 3.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279774, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (365.34, 156.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674490 reads in 201.728 CPU sec, 6.486 real sec
[M::process] read 673314 sequences (100000031 bp) in 2.430 CPU sec, 2.504 real sec...
[M::mem_process_seqs] Processed 674002 reads in 131.907 CPU sec, 4.205 real sec
sorting block 120, #reads = 1068342 mem = 536880146 peak = 1093890048
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279499, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 355, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (367.86, 155.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673596 sequences (100000023 bp) in 2.570 CPU sec, 3.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279282, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 362, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (375.00, 160.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672950 sequences (100000295 bp) in 2.152 CPU sec, 2.222 real sec...
[M::mem_process_seqs] Processed 673572 reads in 201.170 CPU sec, 6.490 real sec
sorting block 121, #reads = 1068506 mem = 536878575 peak = 1093890048
[M::mem_process_seqs] Processed 673314 reads in 130.187 CPU sec, 4.175 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279591, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 360, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (372.73, 159.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 122, #reads = 1068759 mem = 536880753 peak = 1098600448
[M::process] read 672940 sequences (100000236 bp) in 2.674 CPU sec, 3.257 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 278994, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 366, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (378.86, 161.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673436 sequences (100000244 bp) in 2.183 CPU sec, 2.212 real sec...
[M::mem_process_seqs] Processed 673596 reads in 209.286 CPU sec, 6.765 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279854, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 366, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (379.23, 161.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672280 sequences (100000237 bp) in 2.211 CPU sec, 2.215 real sec...
[M::mem_process_seqs] Processed 672940 reads in 114.127 CPU sec, 3.666 real sec
sorting block 123, #reads = 1067573 mem = 536877552 peak = 1098600448
[M::mem_process_seqs] Processed 672950 reads in 283.465 CPU sec, 9.098 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279116, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 352, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (369.72, 160.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 124, #reads = 1069250 mem = 536883874 peak = 1101938688
[M::process] read 671790 sequences (100000300 bp) in 2.886 CPU sec, 3.375 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279658, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 363, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (376.42, 158.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673436 reads in 223.021 CPU sec, 7.169 real sec
[M::process] read 671736 sequences (100000140 bp) in 2.085 CPU sec, 2.178 real sec...
[M::mem_process_seqs] Processed 672280 reads in 130.504 CPU sec, 4.159 real sec
sorting block 125, #reads = 1069360 mem = 536878362 peak = 1101938688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278700, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 369, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (382.78, 161.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671748 sequences (100000059 bp) in 2.683 CPU sec, 3.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278599, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 369, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (382.64, 160.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671834 sequences (100000016 bp) in 1.897 CPU sec, 2.094 real sec...
[M::mem_process_seqs] Processed 671790 reads in 204.085 CPU sec, 6.556 real sec
[M::mem_process_seqs] Processed 671736 reads in 128.078 CPU sec, 4.101 real sec
sorting block 126, #reads = 1069511 mem = 536878577 peak = 1101938688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 277615, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 368, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (381.69, 159.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671694 sequences (100000217 bp) in 2.638 CPU sec, 3.079 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277993, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 365, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (380.47, 160.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671276 sequences (100000131 bp) in 1.976 CPU sec, 2.001 real sec...
[M::mem_process_seqs] Processed 671748 reads in 203.173 CPU sec, 6.554 real sec
[M::mem_process_seqs] Processed 671834 reads in 126.732 CPU sec, 4.058 real sec
sorting block 127, #reads = 1070909 mem = 536883026 peak = 1101938688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 278114, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 362, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (378.37, 159.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671220 sequences (100000039 bp) in 2.911 CPU sec, 3.192 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 277893, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 368, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (384.35, 162.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 671058 sequences (100000109 bp) in 1.891 CPU sec, 1.894 real sec...
[M::mem_process_seqs] Processed 671694 reads in 189.985 CPU sec, 6.064 real sec
sorting block 128, #reads = 1070489 mem = 536882691 peak = 1101938688
[M::mem_process_seqs] Processed 671276 reads in 109.306 CPU sec, 3.458 real sec
sorting block 129, #reads = 1071172 mem = 536885173 peak = 1101938688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 278099, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 369, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (386.14, 164.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670792 sequences (100000244 bp) in 2.504 CPU sec, 2.584 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 277784, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 381, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (397.73, 170.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670670 sequences (100000112 bp) in 1.890 CPU sec, 1.889 real sec...
[M::mem_process_seqs] Processed 671220 reads in 164.132 CPU sec, 5.201 real sec
[M::mem_process_seqs] Processed 671058 reads in 106.564 CPU sec, 3.358 real sec
sorting block 130, #reads = 1072820 mem = 536879891 peak = 1101938688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 278408, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 381, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (398.71, 171.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 670538 sequences (100000074 bp) in 2.514 CPU sec, 2.868 real sec...
[M::process] read 221644 sequences (33007558 bp) in 0.824 CPU sec, 0.828 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 278921, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 387, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (404.11, 175.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670792 reads in 179.581 CPU sec, 5.810 real sec
[M::mem_process_seqs] Processed 670670 reads in 115.389 CPU sec, 3.726 real sec
sorting block 131, #reads = 1072191 mem = 536877842 peak = 1101938688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 277863, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 390, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (408.05, 178.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 91966, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 377, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (395.71, 173.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 670538 reads in 129.091 CPU sec, 4.231 real sec
[M::mem_process_seqs] Processed 221644 reads in 51.811 CPU sec, 1.678 real sec
sorting block 132, #reads = 1073724 mem = 536878414 peak = 1101938688
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 142631356
overall: 69839695872 mem 23513.643 user 537.927 sys 794.483 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_Kh-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:659_Kh-T1-TDNA-1_A23MHWWLT4_1\tLB:659_Kh-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_Kh-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_Kh-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 794.521 sec; CPU: 24051.573 sec
sorting block 133, #reads = 592833 mem = 297626030 peak = 1101938688
uniq header count 1
merging 134 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8295531395
output reads: 143326783
bam_mem_sort: 134 calls 94.479 user 1.711 sys 99.997 real
bam_write: 134 calls 86.346 user 5.810 sys 182.106 real
execute: 1 calls 46.461 user 2.982 sys 856.869 real
merge_files: 1 calls 46.436 user 2.961 sys 61.880 real
parse_chunk: 2137 calls 98.958 user 27.012 sys 164.805 real
read_chunk: 5756 calls 55.144 user 31.425 sys 740.812 real
sort_block: 1 calls 35.983 user 30.326 sys 711.462 real
write_chunk: 3617 calls 46.061 user 2.566 sys 60.631 real
overall: 3213729792 mem 2319.158 user 90.792 sys 856.892 real
ls: cannot access '*.cram': No such file or directory