cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 2348.629 CPU sec, 1042.533 real sec
[M::process] read 704142 sequences (100000153 bp) in 0.999 CPU sec, 1.006 real sec...
[M::process] read 701640 sequences (100000271 bp) in 1.290 CPU sec, 1.287 real sec...
[M::process] read 701028 sequences (100000265 bp) in 1.530 CPU sec, 1.529 real sec...
[M::process] read 700734 sequences (100000272 bp) in 1.489 CPU sec, 1.489 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292388, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (122, 164, 170)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (26, 266)
[M::mem_pestat] mean and std.dev: (145.89, 31.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 314)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (235.26, 111.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292477, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.08, 115.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292027, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (244.36, 116.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704142 reads in 300.079 CPU sec, 9.672 real sec
[M::mem_process_seqs] Processed 701640 reads in 170.380 CPU sec, 5.632 real sec
[M::mem_process_seqs] Processed 701028 reads in 184.187 CPU sec, 6.120 real sec
sorting block 0, #reads = 1095648 mem = 536884139 peak = 576356352
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291798, 7, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 272, 595)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1465)
[M::mem_pestat] mean and std.dev: (299.18, 186.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1900)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.96, 118.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699936 sequences (100000209 bp) in 2.493 CPU sec, 2.750 real sec...
[M::mem_process_seqs] Processed 700734 reads in 89.120 CPU sec, 2.970 real sec
sorting block 1, #reads = 1094910 mem = 536878713 peak = 705331200
[M::process] read 699328 sequences (100000084 bp) in 2.042 CPU sec, 2.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291449, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 168, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (199.73, 80.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 930)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.23, 119.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699146 sequences (100000097 bp) in 1.599 CPU sec, 1.648 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 291180, 7, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 315, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (236.71, 119.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.12, 119.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698416 sequences (100000175 bp) in 1.342 CPU sec, 1.351 real sec...
[M::mem_process_seqs] Processed 699936 reads in 160.218 CPU sec, 5.102 real sec
[M::mem_process_seqs] Processed 699328 reads in 100.400 CPU sec, 3.204 real sec
sorting block 2, #reads = 1094401 mem = 536883702 peak = 705331200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291671, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 421, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1110)
[M::mem_pestat] mean and std.dev: (415.30, 223.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1405)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.77, 121.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698368 sequences (100000277 bp) in 2.864 CPU sec, 3.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290866, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 226, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498)
[M::mem_pestat] mean and std.dev: (223.55, 86.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 607)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.96, 121.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699146 reads in 159.699 CPU sec, 5.111 real sec
[M::process] read 697850 sequences (100000295 bp) in 1.638 CPU sec, 1.673 real sec...
[M::mem_process_seqs] Processed 698416 reads in 101.065 CPU sec, 3.227 real sec
sorting block 3, #reads = 1094266 mem = 536878227 peak = 705331200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290843, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.47, 122.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 4, #reads = 1094236 mem = 536880206 peak = 812306432
[M::process] read 696934 sequences (100000028 bp) in 2.233 CPU sec, 2.379 real sec...
[M::process] read 696982 sequences (100000246 bp) in 1.670 CPU sec, 1.673 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290809, 8, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.38, 121.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 225, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522)
[M::mem_pestat] mean and std.dev: (211.18, 72.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 638)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698368 reads in 161.922 CPU sec, 5.178 real sec
[M::mem_process_seqs] Processed 697850 reads in 101.433 CPU sec, 3.242 real sec
sorting block 5, #reads = 1094133 mem = 536878307 peak = 812306432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290104, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.50, 119.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 335, 946)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2488)
[M::mem_pestat] mean and std.dev: (427.50, 333.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3259)
[M::mem_pestat] skip orientation RR
[M::process] read 697296 sequences (100000110 bp) in 3.445 CPU sec, 3.779 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290538, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.33, 118.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696934 reads in 163.799 CPU sec, 5.255 real sec
[M::mem_process_seqs] Processed 696982 reads in 102.423 CPU sec, 3.267 real sec
sorting block 6, #reads = 1091569 mem = 536877807 peak = 812306432
[M::process] read 697218 sequences (100000230 bp) in 2.925 CPU sec, 3.192 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290362, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (541, 1632, 2334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5920)
[M::mem_pestat] mean and std.dev: (1531.00, 1144.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7713)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (250.28, 117.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 252, 2579)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7459)
[M::mem_pestat] mean and std.dev: (1686.58, 2306.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10912)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290295, 7, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 325, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (314.70, 169.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (250.47, 117.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696944 sequences (100000075 bp) in 2.769 CPU sec, 3.016 real sec...
[M::mem_process_seqs] Processed 697296 reads in 162.728 CPU sec, 5.251 real sec
[M::mem_process_seqs] Processed 697218 reads in 101.894 CPU sec, 3.262 real sec
sorting block 7, #reads = 1092321 mem = 536877349 peak = 812306432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290389, 7, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 200, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (193.50, 92.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (250.49, 117.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696736 sequences (100000057 bp) in 3.199 CPU sec, 3.576 real sec...
[M::mem_process_seqs] Processed 696944 reads in 90.654 CPU sec, 3.036 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290315, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.93, 118.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 8, #reads = 1092222 mem = 536886515 peak = 812306432
[M::mem_process_seqs] Processed 696736 reads in 93.225 CPU sec, 3.132 real sec
[M::process] read 696050 sequences (100000125 bp) in 3.195 CPU sec, 3.570 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 289736, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 336, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (304.92, 105.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.85, 120.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699522 sequences (100000206 bp) in 2.684 CPU sec, 2.942 real sec...
[M::mem_process_seqs] Processed 696050 reads in 95.895 CPU sec, 3.193 real sec
sorting block 9, #reads = 1091439 mem = 536878038 peak = 812306432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291150, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (246.78, 117.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702446 sequences (100000111 bp) in 2.885 CPU sec, 3.232 real sec...
[M::mem_process_seqs] Processed 699522 reads in 94.275 CPU sec, 3.122 real sec
sorting block 10, #reads = 1089111 mem = 536881661 peak = 812306432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292469, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.56, 115.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702446 reads in 85.717 CPU sec, 2.847 real sec
[M::process] read 701440 sequences (100000196 bp) in 2.924 CPU sec, 3.216 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291729, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 313, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (222.50, 94.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.60, 116.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701440 reads in 82.810 CPU sec, 2.738 real sec
sorting block 11, #reads = 1094288 mem = 536877847 peak = 812306432
[M::process] read 701072 sequences (100000276 bp) in 3.507 CPU sec, 3.906 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291456, 4, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.63, 118.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 625, 1272)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3504)
[M::mem_pestat] mean and std.dev: (714.79, 705.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4620)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701072 reads in 84.183 CPU sec, 2.743 real sec
sorting block 12, #reads = 1096010 mem = 536880165 peak = 812306432
[M::process] read 700464 sequences (100000178 bp) in 3.584 CPU sec, 4.024 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291853, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (247.79, 119.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 394, 1628)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4410)
[M::mem_pestat] mean and std.dev: (832.58, 901.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5801)
[M::mem_pestat] skip orientation RR
[M::process] read 699628 sequences (100000079 bp) in 2.485 CPU sec, 2.703 real sec...
[M::mem_process_seqs] Processed 700464 reads in 84.046 CPU sec, 2.723 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291219, 7, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 316, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068)
[M::mem_pestat] mean and std.dev: (279.08, 136.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1365)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.46, 120.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699628 reads in 85.466 CPU sec, 2.783 real sec
sorting block 13, #reads = 1094568 mem = 536879304 peak = 812306432
[M::process] read 699176 sequences (100000039 bp) in 3.387 CPU sec, 3.798 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291423, 1, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 228, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (30, 385)
[M::mem_pestat] mean and std.dev: (187.90, 58.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 456)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.38, 120.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699082 sequences (100000192 bp) in 2.527 CPU sec, 2.737 real sec...
[M::mem_process_seqs] Processed 699176 reads in 85.264 CPU sec, 2.774 real sec
sorting block 14, #reads = 1093311 mem = 536879957 peak = 812306432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 291113, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (218, 379, 937)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2375)
[M::mem_pestat] mean and std.dev: (501.43, 328.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3094)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.36, 121.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699082 reads in 84.611 CPU sec, 2.755 real sec
[M::process] read 698498 sequences (100000002 bp) in 3.246 CPU sec, 3.603 real sec...
sorting block 15, #reads = 1094064 mem = 536877777 peak = 812306432
[M::process] read 697734 sequences (100000159 bp) in 2.059 CPU sec, 2.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291171, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (253.30, 122.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697162 sequences (100000013 bp) in 1.440 CPU sec, 1.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290448, 7, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (296, 452, 1000)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2408)
[M::mem_pestat] mean and std.dev: (616.14, 497.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3112)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.43, 122.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698498 reads in 154.608 CPU sec, 4.883 real sec
[M::process] read 696810 sequences (100000195 bp) in 1.646 CPU sec, 1.685 real sec...
[M::mem_process_seqs] Processed 697734 reads in 95.920 CPU sec, 3.033 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290404, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (273, 423, 571)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167)
[M::mem_pestat] mean and std.dev: (375.50, 135.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1465)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.07, 120.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 16, #reads = 1093025 mem = 536878688 peak = 812306432
[M::process] read 696694 sequences (100000090 bp) in 2.847 CPU sec, 3.172 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290587, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.95, 119.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697162 reads in 155.290 CPU sec, 5.003 real sec
[M::mem_process_seqs] Processed 696810 reads in 98.602 CPU sec, 3.144 real sec
[M::process] read 697260 sequences (100000009 bp) in 2.509 CPU sec, 2.753 real sec...
sorting block 17, #reads = 1092962 mem = 536880056 peak = 812306432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290703, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 203, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (247.50, 97.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 865)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.03, 118.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696974 sequences (100000032 bp) in 2.310 CPU sec, 2.715 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290464, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.14, 118.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696694 reads in 157.352 CPU sec, 5.053 real sec
[M::mem_process_seqs] Processed 697260 reads in 100.521 CPU sec, 3.210 real sec
sorting block 18, #reads = 1092446 mem = 536878699 peak = 812306432
[M::process] read 697572 sequences (100000153 bp) in 2.795 CPU sec, 3.060 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290396, 6, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.42, 118.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 239, 2448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7076)
[M::mem_pestat] mean and std.dev: (948.35, 1137.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9390)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291019, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.19, 118.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696974 reads in 158.258 CPU sec, 5.119 real sec
[M::process] read 697396 sequences (100000103 bp) in 2.716 CPU sec, 2.970 real sec...
[M::mem_process_seqs] Processed 697572 reads in 100.764 CPU sec, 3.231 real sec
sorting block 19, #reads = 1091699 mem = 536878790 peak = 812306432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290269, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.38, 119.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697396 reads in 88.095 CPU sec, 2.947 real sec
[M::process] read 697028 sequences (100000020 bp) in 3.449 CPU sec, 3.840 real sec...
sorting block 20, #reads = 1091757 mem = 536884652 peak = 812306432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290065, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 332, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579)
[M::mem_pestat] mean and std.dev: (262.62, 58.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 698)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.83, 121.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697028 reads in 90.200 CPU sec, 2.993 real sec
[M::process] read 703544 sequences (100000301 bp) in 3.563 CPU sec, 3.955 real sec...
sorting block 21, #reads = 1090736 mem = 536879529 peak = 812306432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292443, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 187, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (233.90, 115.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.22, 114.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 703418 sequences (100000099 bp) in 2.421 CPU sec, 2.627 real sec...
[M::mem_process_seqs] Processed 703544 reads in 87.326 CPU sec, 2.834 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292468, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (253, 332, 608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1318)
[M::mem_pestat] mean and std.dev: (426.55, 218.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1673)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (238.65, 114.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 22, #reads = 1093087 mem = 536878934 peak = 812306432
[M::mem_process_seqs] Processed 703418 reads in 85.788 CPU sec, 2.787 real sec
[M::process] read 702758 sequences (100000257 bp) in 3.423 CPU sec, 3.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292663, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.55, 115.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702758 reads in 86.783 CPU sec, 2.825 real sec
sorting block 23, #reads = 1096716 mem = 536879582 peak = 812306432
[M::process] read 702284 sequences (100000051 bp) in 3.406 CPU sec, 3.782 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292584, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.26, 116.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700850 sequences (100000161 bp) in 2.502 CPU sec, 2.717 real sec...
[M::mem_process_seqs] Processed 702284 reads in 86.527 CPU sec, 2.804 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291701, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.94, 118.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700850 reads in 85.416 CPU sec, 2.772 real sec
sorting block 24, #reads = 1095700 mem = 536878594 peak = 812306432
[M::process] read 701040 sequences (100000125 bp) in 3.316 CPU sec, 3.684 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291638, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.17, 119.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701040 reads in 86.934 CPU sec, 2.837 real sec
[M::process] read 700116 sequences (100000223 bp) in 2.473 CPU sec, 2.896 real sec...
sorting block 25, #reads = 1095199 mem = 536877677 peak = 812306432
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291947, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.18, 119.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700116 reads in 82.831 CPU sec, 2.703 real sec
[M::process] read 699238 sequences (100000148 bp) in 3.453 CPU sec, 3.875 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291220, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.53, 121.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699238 reads in 81.694 CPU sec, 2.663 real sec
sorting block 26, #reads = 1094970 mem = 536878514 peak = 812306432
[M::process] read 699182 sequences (100000227 bp) in 3.380 CPU sec, 3.747 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291060, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.80, 121.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698872 sequences (100000071 bp) in 2.019 CPU sec, 2.315 real sec...
[M::mem_process_seqs] Processed 699182 reads in 82.019 CPU sec, 2.664 real sec
sorting block 27, #reads = 1095205 mem = 536879152 peak = 820523008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291122, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 179, 206)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (68, 298)
[M::mem_pestat] mean and std.dev: (178.00, 29.94)
[M::mem_pestat] low and high boundaries for proper pairs: (22, 344)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.60, 121.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 264, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (57, 457)
[M::mem_pestat] mean and std.dev: (226.89, 60.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 537)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698996 sequences (100000040 bp) in 2.488 CPU sec, 2.747 real sec...
[M::mem_process_seqs] Processed 698872 reads in 83.812 CPU sec, 2.729 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291405, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (246.80, 116.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699054 sequences (100000119 bp) in 2.539 CPU sec, 2.792 real sec...
[M::mem_process_seqs] Processed 698996 reads in 85.027 CPU sec, 2.758 real sec
sorting block 28, #reads = 1094069 mem = 536879957 peak = 820523008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291620, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.52, 119.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699054 reads in 85.026 CPU sec, 2.771 real sec
[M::process] read 697142 sequences (100000148 bp) in 3.383 CPU sec, 3.750 real sec...
sorting block 29, #reads = 1094127 mem = 536880367 peak = 820523008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291078, 8, 19)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.42, 121.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 237, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (262.72, 141.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697142 reads in 84.886 CPU sec, 2.763 real sec
[M::process] read 697102 sequences (100000076 bp) in 3.613 CPU sec, 4.060 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290250, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.07, 120.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697238 sequences (100000154 bp) in 2.495 CPU sec, 2.682 real sec...
[M::mem_process_seqs] Processed 697102 reads in 83.920 CPU sec, 2.722 real sec
sorting block 30, #reads = 1092760 mem = 536884846 peak = 820523008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290620, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.68, 120.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697238 reads in 84.620 CPU sec, 2.762 real sec
[M::process] read 697658 sequences (100000143 bp) in 3.465 CPU sec, 3.841 real sec...
sorting block 31, #reads = 1091981 mem = 536879408 peak = 820523008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290475, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.52, 119.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696974 sequences (100000140 bp) in 2.359 CPU sec, 2.526 real sec...
[M::mem_process_seqs] Processed 697658 reads in 85.449 CPU sec, 2.778 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290335, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (255.18, 122.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696974 reads in 84.444 CPU sec, 2.738 real sec
[M::process] read 701978 sequences (100000039 bp) in 3.416 CPU sec, 3.801 real sec...
sorting block 32, #reads = 1090753 mem = 536882201 peak = 820523008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291902, 9, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (277, 364, 1420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3706)
[M::mem_pestat] mean and std.dev: (809.73, 972.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4849)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.98, 115.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701978 reads in 85.080 CPU sec, 2.783 real sec
[M::process] read 703196 sequences (100000212 bp) in 3.212 CPU sec, 3.671 real sec...
sorting block 33, #reads = 1092441 mem = 536879832 peak = 820523008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292323, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (238.65, 114.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703196 reads in 81.602 CPU sec, 2.655 real sec
[M::process] read 702646 sequences (100000053 bp) in 3.033 CPU sec, 3.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292300, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.27, 114.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702646 reads in 82.510 CPU sec, 2.693 real sec
[M::process] read 702766 sequences (100000063 bp) in 3.272 CPU sec, 3.779 real sec...
sorting block 34, #reads = 1096961 mem = 536883703 peak = 820523008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292370, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.89, 116.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702766 reads in 83.204 CPU sec, 2.711 real sec
[M::process] read 701864 sequences (100000296 bp) in 2.719 CPU sec, 2.983 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292049, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.77, 116.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701864 reads in 82.913 CPU sec, 2.713 real sec
sorting block 35, #reads = 1096528 mem = 536878668 peak = 820523008
[M::process] read 701354 sequences (100000000 bp) in 2.898 CPU sec, 3.203 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292376, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.38, 118.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701354 reads in 83.099 CPU sec, 2.705 real sec
[M::process] read 700878 sequences (100000032 bp) in 2.358 CPU sec, 2.811 real sec...
sorting block 36, #reads = 1095703 mem = 536878410 peak = 820523008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291810, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.69, 118.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700878 reads in 83.173 CPU sec, 2.720 real sec
[M::process] read 699908 sequences (100000102 bp) in 3.309 CPU sec, 3.684 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291825, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.07, 119.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699908 reads in 82.715 CPU sec, 2.695 real sec
sorting block 37, #reads = 1095345 mem = 536880282 peak = 820523008
[M::process] read 699816 sequences (100000003 bp) in 3.336 CPU sec, 3.730 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291236, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 249, 1034)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2740)
[M::mem_pestat] mean and std.dev: (385.00, 348.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3593)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.39, 121.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699378 sequences (100000262 bp) in 2.549 CPU sec, 2.735 real sec...
[M::mem_process_seqs] Processed 699816 reads in 83.813 CPU sec, 2.737 real sec
sorting block 38, #reads = 1094530 mem = 536879218 peak = 820523008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290985, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.25, 122.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 384, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104)
[M::mem_pestat] mean and std.dev: (280.54, 159.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1422)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699378 reads in 82.874 CPU sec, 2.704 real sec
[M::process] read 698868 sequences (100000300 bp) in 2.509 CPU sec, 2.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291389, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.33, 121.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698868 reads in 83.472 CPU sec, 2.725 real sec
[M::process] read 697864 sequences (100000164 bp) in 3.145 CPU sec, 3.509 real sec...
sorting block 39, #reads = 1094625 mem = 536880262 peak = 820523008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290767, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.08, 121.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697864 reads in 84.208 CPU sec, 2.754 real sec
[M::process] read 697092 sequences (100000193 bp) in 3.373 CPU sec, 3.816 real sec...
sorting block 40, #reads = 1094463 mem = 536878513 peak = 820523008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290889, 5, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (255.14, 122.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 263, 1291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3463)
[M::mem_pestat] mean and std.dev: (598.83, 651.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4549)
[M::mem_pestat] skip orientation RR
[M::process] read 696894 sequences (100000203 bp) in 2.275 CPU sec, 2.573 real sec...
[M::mem_process_seqs] Processed 697092 reads in 84.668 CPU sec, 2.750 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290986, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.25, 121.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696894 reads in 84.677 CPU sec, 2.759 real sec
[M::process] read 696538 sequences (100000159 bp) in 3.382 CPU sec, 3.710 real sec...
sorting block 41, #reads = 1093523 mem = 536875206 peak = 820523008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289888, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (255.59, 121.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696538 reads in 85.392 CPU sec, 2.795 real sec
[M::process] read 696506 sequences (100000135 bp) in 3.203 CPU sec, 3.777 real sec...
sorting block 42, #reads = 1091741 mem = 536880101 peak = 820523008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290345, 6, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 212, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (257.36, 108.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.47, 120.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 187, 3150)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9092)
[M::mem_pestat] mean and std.dev: (1174.33, 1618.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12063)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 696082 sequences (100000080 bp) in 2.299 CPU sec, 2.722 real sec...
[M::mem_process_seqs] Processed 696506 reads in 85.464 CPU sec, 2.777 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290411, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.66, 120.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696082 reads in 85.868 CPU sec, 2.802 real sec
[M::process] read 700348 sequences (100000205 bp) in 3.361 CPU sec, 3.717 real sec...
sorting block 43, #reads = 1090379 mem = 536886222 peak = 820523008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291350, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (137, 198, 896)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2414)
[M::mem_pestat] mean and std.dev: (391.46, 364.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3173)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (243.91, 116.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 706580 sequences (100000208 bp) in 2.416 CPU sec, 2.898 real sec...
[M::mem_process_seqs] Processed 700348 reads in 91.351 CPU sec, 2.969 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293685, 9, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (228.50, 108.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 244, 570)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1408)
[M::mem_pestat] mean and std.dev: (350.27, 271.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1827)
[M::mem_pestat] skip orientation RR
[M::process] read 706292 sequences (100000290 bp) in 2.579 CPU sec, 2.853 real sec...
[M::mem_process_seqs] Processed 706580 reads in 89.391 CPU sec, 2.908 real sec
sorting block 44, #reads = 1089396 mem = 536878431 peak = 851410944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 293737, 1, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (268, 456, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (345.70, 170.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (229.96, 109.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 706292 reads in 88.734 CPU sec, 2.892 real sec
[M::process] read 704158 sequences (100000007 bp) in 3.457 CPU sec, 3.837 real sec...
sorting block 45, #reads = 1096281 mem = 536880852 peak = 851410944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292738, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (235.81, 113.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704158 reads in 87.352 CPU sec, 2.848 real sec
[M::process] read 703088 sequences (100000175 bp) in 3.047 CPU sec, 3.445 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292556, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (239.50, 116.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 247, 267)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (96, 381)
[M::mem_pestat] mean and std.dev: (225.40, 52.74)
[M::mem_pestat] low and high boundaries for proper pairs: (14, 438)
[M::mem_pestat] skip orientation RR
[M::process] read 702178 sequences (100000028 bp) in 2.375 CPU sec, 2.693 real sec...
[M::mem_process_seqs] Processed 703088 reads in 86.593 CPU sec, 2.820 real sec
sorting block 46, #reads = 1094120 mem = 536878335 peak = 872390656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291957, 5, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (248, 551, 799)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1901)
[M::mem_pestat] mean and std.dev: (510.60, 285.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2452)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.01, 118.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701474 sequences (100000236 bp) in 2.529 CPU sec, 2.824 real sec...
[M::mem_process_seqs] Processed 702178 reads in 85.946 CPU sec, 2.799 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292287, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (244.89, 119.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1095533 mem = 536880056 peak = 884453376
[M::mem_process_seqs] Processed 701474 reads in 86.417 CPU sec, 2.822 real sec
[M::process] read 700676 sequences (100000047 bp) in 3.330 CPU sec, 3.714 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 291894, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (247.73, 121.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701016 sequences (100000154 bp) in 2.514 CPU sec, 2.767 real sec...
[M::mem_process_seqs] Processed 700676 reads in 87.395 CPU sec, 2.846 real sec
sorting block 48, #reads = 1094577 mem = 536880176 peak = 884453376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291564, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.83, 121.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699262 sequences (100000097 bp) in 2.561 CPU sec, 2.837 real sec...
[M::mem_process_seqs] Processed 701016 reads in 87.610 CPU sec, 2.865 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291050, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.51, 120.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1093454 mem = 536879906 peak = 884453376
[M::process] read 698796 sequences (100000272 bp) in 2.569 CPU sec, 2.891 real sec...
[M::mem_process_seqs] Processed 699262 reads in 89.224 CPU sec, 2.910 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290689, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (250.13, 121.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 312, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1183)
[M::mem_pestat] mean and std.dev: (348.92, 190.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1502)
[M::mem_pestat] skip orientation RR
[M::process] read 698296 sequences (100000199 bp) in 2.491 CPU sec, 2.807 real sec...
[M::mem_process_seqs] Processed 698796 reads in 88.836 CPU sec, 2.899 real sec
sorting block 50, #reads = 1091100 mem = 536885285 peak = 884453376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291089, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.90, 120.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698656 sequences (100000008 bp) in 2.522 CPU sec, 2.802 real sec...
[M::mem_process_seqs] Processed 698296 reads in 88.412 CPU sec, 2.904 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290966, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.81, 119.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698558 sequences (100000087 bp) in 2.612 CPU sec, 2.929 real sec...
[M::mem_process_seqs] Processed 698656 reads in 87.871 CPU sec, 2.875 real sec
sorting block 51, #reads = 1090809 mem = 536882047 peak = 884453376
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291248, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 363, 919)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2273)
[M::mem_pestat] mean and std.dev: (639.20, 583.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2973)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.48, 119.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698814 sequences (100000168 bp) in 2.560 CPU sec, 2.857 real sec...
[M::mem_process_seqs] Processed 698558 reads in 89.749 CPU sec, 2.934 real sec
sorting block 52, #reads = 1090185 mem = 536880237 peak = 891953152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290998, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.23, 119.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698814 reads in 89.016 CPU sec, 2.911 real sec
[M::process] read 698114 sequences (100000277 bp) in 3.186 CPU sec, 3.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290666, 11, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.90, 120.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (853, 943, 1613)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3133)
[M::mem_pestat] mean and std.dev: (1105.18, 743.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4078)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 698114 reads in 89.088 CPU sec, 2.907 real sec
sorting block 53, #reads = 1089609 mem = 536879434 peak = 891953152
[M::process] read 698068 sequences (100000191 bp) in 3.046 CPU sec, 3.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290554, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.85, 119.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698068 reads in 91.523 CPU sec, 2.974 real sec
[M::process] read 707020 sequences (100000129 bp) in 3.508 CPU sec, 3.890 real sec...
sorting block 54, #reads = 1090124 mem = 536882220 peak = 891953152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293261, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (228.34, 108.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 273, 1285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3429)
[M::mem_pestat] mean and std.dev: (460.82, 466.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4501)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 707020 reads in 99.066 CPU sec, 3.217 real sec
[M::process] read 707256 sequences (100000222 bp) in 2.891 CPU sec, 3.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 294077, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 204, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585)
[M::mem_pestat] mean and std.dev: (227.56, 107.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 731)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 706920 sequences (100000144 bp) in 2.438 CPU sec, 2.796 real sec...
[M::mem_process_seqs] Processed 707256 reads in 95.802 CPU sec, 3.113 real sec
sorting block 55, #reads = 1088830 mem = 536878098 peak = 891953152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 293745, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (229.03, 108.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705368 sequences (100000200 bp) in 2.577 CPU sec, 2.892 real sec...
[M::mem_process_seqs] Processed 706920 reads in 96.739 CPU sec, 3.141 real sec
sorting block 56, #reads = 1091071 mem = 536878995 peak = 891953152
[M::process] read 704426 sequences (100000163 bp) in 2.410 CPU sec, 2.667 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293074, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (232.74, 110.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703076 sequences (100000288 bp) in 1.655 CPU sec, 1.757 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293316, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (235.30, 112.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701916 sequences (100000018 bp) in 1.470 CPU sec, 1.474 real sec...
[M::mem_process_seqs] Processed 705368 reads in 170.314 CPU sec, 5.385 real sec
[M::mem_process_seqs] Processed 704426 reads in 109.200 CPU sec, 3.482 real sec
sorting block 57, #reads = 1089254 mem = 536879102 peak = 891953152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292211, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (239.36, 115.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (403, 772, 957)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2065)
[M::mem_pestat] mean and std.dev: (650.23, 307.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2619)
[M::mem_pestat] skip orientation RR
[M::process] read 701434 sequences (100000203 bp) in 2.691 CPU sec, 3.039 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292092, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.20, 115.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703076 reads in 165.192 CPU sec, 5.302 real sec
[M::mem_process_seqs] Processed 701916 reads in 106.974 CPU sec, 3.424 real sec
[M::process] read 701342 sequences (100000178 bp) in 2.188 CPU sec, 2.289 real sec...
sorting block 58, #reads = 1091746 mem = 536878726 peak = 891953152
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292042, 7, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (243.36, 117.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 285, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (134, 394)
[M::mem_pestat] mean and std.dev: (250.80, 51.80)
[M::mem_pestat] low and high boundaries for proper pairs: (44, 458)
[M::mem_pestat] skip orientation RR
[M::process] read 700040 sequences (100000169 bp) in 2.742 CPU sec, 2.992 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291762, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.84, 118.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701434 reads in 160.157 CPU sec, 5.124 real sec
[M::mem_process_seqs] Processed 701342 reads in 102.714 CPU sec, 3.272 real sec
sorting block 59, #reads = 1091063 mem = 536880829 peak = 894124032
[M::process] read 699334 sequences (100000077 bp) in 2.175 CPU sec, 2.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291524, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (246.51, 118.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1091371 mem = 536877721 peak = 945754112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290895, 6, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (146, 175, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094)
[M::mem_pestat] mean and std.dev: (218.62, 115.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1410)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (248.00, 119.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700040 reads in 159.399 CPU sec, 5.170 real sec
[M::process] read 698550 sequences (100000061 bp) in 3.372 CPU sec, 3.765 real sec...
[M::mem_process_seqs] Processed 699334 reads in 104.015 CPU sec, 3.346 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291200, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.52, 120.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698656 sequences (100000032 bp) in 2.494 CPU sec, 2.873 real sec...
[M::mem_process_seqs] Processed 698550 reads in 87.931 CPU sec, 2.936 real sec
sorting block 61, #reads = 1091529 mem = 536879712 peak = 945754112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291082, 4, 18)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.81, 120.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 261, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (253.60, 117.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 869)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698656 reads in 88.643 CPU sec, 2.942 real sec
[M::process] read 698540 sequences (100000196 bp) in 2.839 CPU sec, 3.137 real sec...
sorting block 62, #reads = 1090900 mem = 536883147 peak = 945754112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291294, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (249.32, 120.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698540 reads in 88.274 CPU sec, 2.955 real sec
[M::process] read 699038 sequences (100000050 bp) in 3.499 CPU sec, 3.925 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291162, 7, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (105, 157, 189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357)
[M::mem_pestat] mean and std.dev: (149.90, 57.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 441)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (248.00, 120.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 63, #reads = 1090813 mem = 536878926 peak = 945754112
[M::mem_process_seqs] Processed 699038 reads in 89.810 CPU sec, 2.973 real sec
[M::process] read 698802 sequences (100000000 bp) in 3.436 CPU sec, 3.989 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290646, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.42, 120.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 706654 sequences (100000230 bp) in 2.701 CPU sec, 3.004 real sec...
[M::mem_process_seqs] Processed 698802 reads in 98.176 CPU sec, 3.215 real sec
sorting block 64, #reads = 1089632 mem = 536876652 peak = 945754112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293374, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (229.86, 109.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 747)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 706654 reads in 92.589 CPU sec, 3.017 real sec
[M::process] read 706582 sequences (100000234 bp) in 3.290 CPU sec, 3.686 real sec...
sorting block 65, #reads = 1084718 mem = 536881126 peak = 945754112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293626, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 205, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (229.55, 109.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 706582 reads in 88.883 CPU sec, 2.921 real sec
[M::process] read 705046 sequences (100000227 bp) in 3.427 CPU sec, 3.868 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 293461, 6, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 355, 645)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1515)
[M::mem_pestat] mean and std.dev: (311.00, 156.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1950)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (233.41, 111.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 273, 551)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1169)
[M::mem_pestat] mean and std.dev: (362.70, 231.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1478)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 704064 sequences (100000216 bp) in 2.476 CPU sec, 2.822 real sec...
[M::mem_process_seqs] Processed 705046 reads in 88.171 CPU sec, 2.870 real sec
sorting block 66, #reads = 1095754 mem = 536876264 peak = 945754112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292958, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 254, 750)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1888)
[M::mem_pestat] mean and std.dev: (406.77, 340.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2457)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (235.73, 113.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 704064 reads in 86.032 CPU sec, 2.812 real sec
[M::process] read 702508 sequences (100000177 bp) in 3.394 CPU sec, 3.786 real sec...
sorting block 67, #reads = 1095616 mem = 536880555 peak = 945754112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292578, 7, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 265, 843)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085)
[M::mem_pestat] mean and std.dev: (496.00, 533.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2706)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.31, 115.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 702508 reads in 85.408 CPU sec, 2.786 real sec
[M::process] read 702072 sequences (100000200 bp) in 3.026 CPU sec, 3.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292256, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.32, 115.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702072 reads in 84.990 CPU sec, 2.781 real sec
sorting block 68, #reads = 1095573 mem = 536877473 peak = 945754112
[M::process] read 701780 sequences (100000122 bp) in 3.287 CPU sec, 3.824 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291962, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.24, 115.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701454 sequences (100000218 bp) in 2.259 CPU sec, 2.473 real sec...
[M::mem_process_seqs] Processed 701780 reads in 86.718 CPU sec, 2.823 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292121, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.30, 116.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 69, #reads = 1094766 mem = 536879603 peak = 945754112
[M::mem_process_seqs] Processed 701454 reads in 86.022 CPU sec, 2.795 real sec
[M::process] read 701130 sequences (100000182 bp) in 3.088 CPU sec, 3.440 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292135, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.86, 116.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701130 reads in 87.651 CPU sec, 2.880 real sec
[M::process] read 701694 sequences (100000168 bp) in 3.285 CPU sec, 3.682 real sec...
sorting block 70, #reads = 1093751 mem = 536879974 peak = 945754112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292127, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.78, 115.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701628 sequences (100000208 bp) in 2.337 CPU sec, 2.644 real sec...
[M::mem_process_seqs] Processed 701694 reads in 88.087 CPU sec, 2.860 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291798, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 215, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (239.98, 114.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701628 reads in 88.324 CPU sec, 2.882 real sec
sorting block 71, #reads = 1093261 mem = 536884844 peak = 945754112
[M::process] read 701718 sequences (100000203 bp) in 3.252 CPU sec, 3.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291825, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (116, 313, 665)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1763)
[M::mem_pestat] mean and std.dev: (393.22, 477.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2312)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.61, 116.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701718 reads in 88.260 CPU sec, 2.871 real sec
[M::process] read 700340 sequences (100000103 bp) in 2.591 CPU sec, 2.964 real sec...
sorting block 72, #reads = 1092551 mem = 536880935 peak = 945754112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291956, 11, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (233, 372, 3143)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8963)
[M::mem_pestat] mean and std.dev: (1172.83, 1324.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11873)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (245.19, 118.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 219, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695)
[M::mem_pestat] mean and std.dev: (215.30, 119.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 871)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 700340 reads in 87.089 CPU sec, 2.836 real sec
[M::process] read 699522 sequences (100000136 bp) in 3.432 CPU sec, 3.799 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291402, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 238, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497)
[M::mem_pestat] mean and std.dev: (193.25, 71.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 605)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.73, 119.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699522 reads in 87.102 CPU sec, 2.839 real sec
sorting block 73, #reads = 1091821 mem = 536879852 peak = 945754112
[M::process] read 699552 sequences (100000052 bp) in 3.242 CPU sec, 3.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 291564, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.83, 120.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700100 sequences (100000290 bp) in 2.222 CPU sec, 2.399 real sec...
[M::mem_process_seqs] Processed 699552 reads in 87.916 CPU sec, 2.855 real sec
sorting block 74, #reads = 1090396 mem = 536877466 peak = 945754112
[M::process] read 704972 sequences (100000186 bp) in 2.495 CPU sec, 2.750 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291036, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (243.89, 117.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 708984 sequences (100000252 bp) in 1.494 CPU sec, 1.659 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293276, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (233.72, 112.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700100 reads in 172.640 CPU sec, 5.455 real sec
[M::process] read 708796 sequences (100000264 bp) in 1.530 CPU sec, 1.533 real sec...
[M::mem_process_seqs] Processed 704972 reads in 115.260 CPU sec, 3.667 real sec
sorting block 75, #reads = 1089358 mem = 536880511 peak = 945754112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 294351, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577)
[M::mem_pestat] mean and std.dev: (224.26, 105.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 721)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 217, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597)
[M::mem_pestat] mean and std.dev: (247.55, 88.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 733)
[M::mem_pestat] skip orientation RR
[M::process] read 707618 sequences (100000243 bp) in 3.446 CPU sec, 3.867 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 294230, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (342, 603, 1854)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4878)
[M::mem_pestat] mean and std.dev: (864.33, 683.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6390)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (224.11, 105.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 708984 reads in 172.191 CPU sec, 5.518 real sec
sorting block 76, #reads = 1088619 mem = 536886161 peak = 945754112
[M::process] read 706500 sequences (100000142 bp) in 2.346 CPU sec, 2.507 real sec...
[M::process] read 704486 sequences (100000233 bp) in 1.697 CPU sec, 1.706 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293834, 3, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 295, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (280.00, 124.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595)
[M::mem_pestat] mean and std.dev: (230.40, 109.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 744)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 706500 reads in 89.741 CPU sec, 3.075 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 293934, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 203, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581)
[M::mem_pestat] mean and std.dev: (226.27, 106.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 726)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 707618 reads in 192.821 CPU sec, 6.955 real sec
sorting block 77, #reads = 1091149 mem = 536885153 peak = 945754112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 293123, 2, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 198, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (216.10, 73.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (235.04, 112.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 703576 sequences (100000236 bp) in 3.166 CPU sec, 3.470 real sec...
[M::mem_process_seqs] Processed 704486 reads in 107.707 CPU sec, 3.508 real sec
[M::mem_process_seqs] Processed 708796 reads in 388.508 CPU sec, 13.548 real sec
sorting block 78, #reads = 1091101 mem = 536881644 peak = 945754112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292772, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (246, 246, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (231.56, 74.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (237.82, 114.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 703350 sequences (100000157 bp) in 2.443 CPU sec, 2.700 real sec...
[M::mem_process_seqs] Processed 703576 reads in 91.333 CPU sec, 3.050 real sec
sorting block 79, #reads = 1089903 mem = 536878816 peak = 952745984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292619, 7, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (239.13, 115.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 352, 723)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1757)
[M::mem_pestat] mean and std.dev: (402.09, 324.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2274)
[M::mem_pestat] skip orientation RR
[M::process] read 702902 sequences (100000154 bp) in 2.799 CPU sec, 3.150 real sec...
[M::mem_process_seqs] Processed 703350 reads in 90.037 CPU sec, 2.990 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292920, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (239.85, 116.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702902 reads in 88.581 CPU sec, 2.947 real sec
[M::process] read 702334 sequences (100000050 bp) in 3.350 CPU sec, 3.744 real sec...
sorting block 80, #reads = 1092706 mem = 536880221 peak = 952745984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292473, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (241.26, 117.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701342 sequences (100000117 bp) in 2.489 CPU sec, 2.765 real sec...
[M::mem_process_seqs] Processed 702334 reads in 89.145 CPU sec, 2.945 real sec
sorting block 81, #reads = 1093611 mem = 536879587 peak = 952745984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292170, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (244.40, 118.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701016 sequences (100000078 bp) in 2.525 CPU sec, 2.981 real sec...
[M::mem_process_seqs] Processed 701342 reads in 88.274 CPU sec, 2.906 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291904, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (244.88, 118.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699822 sequences (100000062 bp) in 2.609 CPU sec, 2.944 real sec...
[M::mem_process_seqs] Processed 701016 reads in 88.157 CPU sec, 2.900 real sec
sorting block 82, #reads = 1092358 mem = 536878654 peak = 952745984
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291450, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.41, 121.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (105, 176, 241)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513)
[M::mem_pestat] mean and std.dev: (195.92, 108.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 649)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699822 reads in 86.379 CPU sec, 2.837 real sec
[M::process] read 699084 sequences (100000143 bp) in 3.169 CPU sec, 3.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291602, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.56, 121.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 83, #reads = 1092875 mem = 536880638 peak = 952745984
[M::mem_process_seqs] Processed 699084 reads in 85.687 CPU sec, 2.837 real sec
[M::process] read 699006 sequences (100000295 bp) in 3.468 CPU sec, 3.983 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291380, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (249.79, 120.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 84, #reads = 1092254 mem = 536879504 peak = 952745984
[M::mem_process_seqs] Processed 699006 reads in 88.175 CPU sec, 2.873 real sec
[M::process] read 698336 sequences (100000015 bp) in 2.588 CPU sec, 2.885 real sec...
[M::process] read 12622 sequences (1797820 bp) in 0.302 CPU sec, 0.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291085, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 5269, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (243.80, 117.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.00, 119.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 12622 reads in 2.111 CPU sec, 0.070 real sec
[M::mem_process_seqs] Processed 698336 reads in 88.187 CPU sec, 2.881 real sec
sorting block 85, #reads = 1091768 mem = 536879145 peak = 952745984
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 93890482
overall: 69914599424 mem 13293.617 user 943.087 sys 1481.956 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0004.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0004.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1482.014 sec; CPU: 14236.707 sec
sorting block 86, #reads = 395230 mem = 195090398 peak = 952745984
uniq header count 1
merging 87 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 5198695169
output reads: 94370464
bam_mem_sort: 87 calls 34.283 user 0.835 sys 35.272 real
bam_write: 87 calls 80.197 user 1.906 sys 82.538 real
execute: 1 calls 22.065 user 1.611 sys 1511.385 real
merge_files: 1 calls 22.060 user 1.570 sys 28.805 real
parse_chunk: 1382 calls 42.863 user 13.414 sys 57.995 real
read_chunk: 3733 calls 50.562 user 46.273 sys 443.632 real
sort_block: 1 calls 40.665 user 45.595 sys 429.338 real
write_chunk: 2349 calls 21.619 user 1.328 sys 27.825 real
overall: 2875293696 mem 1107.021 user 71.547 sys 1511.407 real
ls: cannot access '*.cram': No such file or directory