This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
Received only 8 licenses out of 31 requested for sentieon:klib
Received only 8 licenses out of 31 requested for sentieon:klib
Received only 8 licenses out of 31 requested for sentieon:klib
[M::main_mem] bwa index loaded in 1535.310 CPU sec, 784.305 real sec
[M::process] read 703682 sequences (100000105 bp) in 0.985 CPU sec, 0.993 real sec...
[M::process] read 702276 sequences (100000150 bp) in 1.183 CPU sec, 1.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292713, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (234.56, 109.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 759)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701870 sequences (100000073 bp) in 1.178 CPU sec, 1.197 real sec...
[M::process] read 701138 sequences (100000130 bp) in 1.169 CPU sec, 1.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292260, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (239.90, 113.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703682 reads in 136.233 CPU sec, 4.348 real sec
[M::mem_process_seqs] Processed 702276 reads in 80.967 CPU sec, 2.608 real sec
sorting block 0, #reads = 1095731 mem = 536878506 peak = 603955200
[M::process] read 700598 sequences (100000149 bp) in 1.590 CPU sec, 1.671 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291737, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (242.96, 115.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699756 sequences (100000122 bp) in 1.170 CPU sec, 1.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 291797, 1, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (127, 212, 988)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2710)
[M::mem_pestat] mean and std.dev: (414.93, 413.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3571)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.27, 116.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291217, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.47, 118.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 287, 1786)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4814)
[M::mem_pestat] mean and std.dev: (770.23, 944.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6328)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701870 reads in 182.260 CPU sec, 5.851 real sec
[M::mem_process_seqs] Processed 701138 reads in 134.570 CPU sec, 4.345 real sec
[M::mem_process_seqs] Processed 700598 reads in 146.197 CPU sec, 4.741 real sec
sorting block 1, #reads = 1094671 mem = 536879256 peak = 729784320
[M::process] read 699330 sequences (100000003 bp) in 1.733 CPU sec, 1.866 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291544, 0, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (222, 279, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 531)
[M::mem_pestat] mean and std.dev: (249.73, 49.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 634)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.40, 119.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 182, 275)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521)
[M::mem_pestat] mean and std.dev: (190.33, 48.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 644)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 2, #reads = 1095464 mem = 536884562 peak = 906485760
[M::process] read 698688 sequences (100000132 bp) in 1.486 CPU sec, 1.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291028, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (249.95, 119.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699756 reads in 129.173 CPU sec, 4.114 real sec
[M::mem_process_seqs] Processed 699330 reads in 81.206 CPU sec, 2.563 real sec
[M::process] read 698732 sequences (100000013 bp) in 1.549 CPU sec, 1.619 real sec...
sorting block 3, #reads = 1093902 mem = 536880306 peak = 906485760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291319, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.05, 121.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698336 sequences (100000205 bp) in 1.605 CPU sec, 1.685 real sec...
[M::process] read 697482 sequences (100000218 bp) in 1.241 CPU sec, 1.252 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291349, 8, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 321, 410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (246.67, 98.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.06, 121.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698688 reads in 127.927 CPU sec, 4.075 real sec
[M::mem_process_seqs] Processed 698732 reads in 80.201 CPU sec, 2.549 real sec
sorting block 4, #reads = 1094462 mem = 536880826 peak = 906485760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291149, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.40, 121.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696500 sequences (100000211 bp) in 1.743 CPU sec, 1.865 real sec...
[M::process] read 696296 sequences (100000154 bp) in 1.300 CPU sec, 1.298 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290690, 10, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.69, 121.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (355, 903, 1394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3472)
[M::mem_pestat] mean and std.dev: (804.70, 580.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4511)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 698336 reads in 128.406 CPU sec, 4.088 real sec
sorting block 5, #reads = 1094744 mem = 536879719 peak = 906485760
[M::mem_process_seqs] Processed 697482 reads in 80.835 CPU sec, 2.567 real sec
sorting block 6, #reads = 1093074 mem = 536881082 peak = 906485760
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290265, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.36, 120.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 244, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559)
[M::mem_pestat] mean and std.dev: (267.17, 95.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 673)
[M::mem_pestat] skip orientation RR
[M::process] read 696398 sequences (100000249 bp) in 1.841 CPU sec, 1.949 real sec...
[M::process] read 696380 sequences (100000045 bp) in 1.290 CPU sec, 1.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290407, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (255.60, 120.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696500 reads in 129.242 CPU sec, 4.111 real sec
[M::mem_process_seqs] Processed 696296 reads in 81.452 CPU sec, 2.584 real sec
sorting block 7, #reads = 1092489 mem = 536878285 peak = 906485760
[M::process] read 695912 sequences (100000018 bp) in 1.735 CPU sec, 1.830 real sec...
[M::process] read 696244 sequences (100000070 bp) in 1.330 CPU sec, 1.327 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290029, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.68, 118.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696380 reads in 71.567 CPU sec, 2.260 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290214, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.59, 119.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289976, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (255.09, 120.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699612 sequences (100000208 bp) in 1.609 CPU sec, 1.617 real sec...
[M::mem_process_seqs] Processed 696398 reads in 202.417 CPU sec, 6.434 real sec
sorting block 8, #reads = 1090959 mem = 536878925 peak = 906485760
[M::mem_process_seqs] Processed 695912 reads in 95.445 CPU sec, 3.045 real sec
sorting block 9, #reads = 1090222 mem = 536878376 peak = 978968576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290207, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.11, 120.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702768 sequences (100000016 bp) in 2.134 CPU sec, 2.249 real sec...
[M::process] read 701178 sequences (100000088 bp) in 1.290 CPU sec, 1.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291927, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (129, 225, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (195.36, 75.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (247.79, 118.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696244 reads in 132.210 CPU sec, 4.200 real sec
[M::mem_process_seqs] Processed 699612 reads in 83.888 CPU sec, 2.661 real sec
sorting block 10, #reads = 1090140 mem = 536879013 peak = 978968576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292241, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (237.84, 112.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701254 sequences (100000051 bp) in 2.461 CPU sec, 2.621 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291188, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (243.26, 115.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (278, 684, 902)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2150)
[M::mem_pestat] mean and std.dev: (442.50, 264.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2774)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702768 reads in 129.164 CPU sec, 4.106 real sec
[M::mem_process_seqs] Processed 701178 reads in 81.706 CPU sec, 2.583 real sec
[M::process] read 700972 sequences (100000277 bp) in 1.715 CPU sec, 1.747 real sec...
sorting block 11, #reads = 1094685 mem = 536883260 peak = 978968576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291318, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (243.41, 115.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700408 sequences (100000214 bp) in 2.255 CPU sec, 2.394 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291474, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (244.68, 116.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701254 reads in 128.498 CPU sec, 4.097 real sec
[M::process] read 699846 sequences (100000041 bp) in 1.488 CPU sec, 1.508 real sec...
[M::mem_process_seqs] Processed 700972 reads in 81.629 CPU sec, 2.576 real sec
sorting block 12, #reads = 1095291 mem = 536880047 peak = 978968576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291389, 10, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.15, 118.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (85, 119, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054)
[M::mem_pestat] mean and std.dev: (133.62, 109.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1377)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 13, #reads = 1095786 mem = 536880047 peak = 978968576
[M::process] read 699056 sequences (100000086 bp) in 2.093 CPU sec, 2.205 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291277, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 292, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (291.40, 163.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.87, 119.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698948 sequences (100000057 bp) in 1.380 CPU sec, 1.375 real sec...
[M::mem_process_seqs] Processed 700408 reads in 128.013 CPU sec, 4.074 real sec
[M::mem_process_seqs] Processed 699846 reads in 80.815 CPU sec, 2.556 real sec
sorting block 14, #reads = 1095302 mem = 536885471 peak = 978968576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290974, 8, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 195, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1222)
[M::mem_pestat] mean and std.dev: (249.42, 145.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1579)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.40, 119.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 367, 1542)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4220)
[M::mem_pestat] mean and std.dev: (727.24, 690.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5559)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698258 sequences (100000056 bp) in 2.255 CPU sec, 2.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291563, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.18, 119.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699056 reads in 127.922 CPU sec, 4.069 real sec
[M::process] read 698600 sequences (100000092 bp) in 1.407 CPU sec, 1.403 real sec...
[M::mem_process_seqs] Processed 698948 reads in 81.141 CPU sec, 2.563 real sec
sorting block 15, #reads = 1094260 mem = 536878231 peak = 978968576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290598, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.77, 120.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697956 sequences (100000081 bp) in 2.008 CPU sec, 2.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290990, 5, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.66, 120.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 258, 1162)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3148)
[M::mem_pestat] mean and std.dev: (652.69, 836.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4141)
[M::mem_pestat] skip orientation RR
[M::process] read 697256 sequences (100000142 bp) in 1.380 CPU sec, 1.377 real sec...
[M::mem_process_seqs] Processed 698258 reads in 129.853 CPU sec, 4.133 real sec
[M::mem_process_seqs] Processed 698600 reads in 82.298 CPU sec, 2.605 real sec
sorting block 16, #reads = 1094484 mem = 536878663 peak = 978968576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290539, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (251.99, 119.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697328 sequences (100000030 bp) in 2.057 CPU sec, 2.204 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290520, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.55, 120.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697956 reads in 130.734 CPU sec, 4.162 real sec
[M::process] read 697134 sequences (100000011 bp) in 1.380 CPU sec, 1.379 real sec...
sorting block 17, #reads = 1093121 mem = 536877238 peak = 978968576
[M::mem_process_seqs] Processed 697256 reads in 82.167 CPU sec, 2.598 real sec
sorting block 18, #reads = 1091592 mem = 536880379 peak = 1024880640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290284, 7, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.74, 118.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 244, 722)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1796)
[M::mem_pestat] mean and std.dev: (267.22, 171.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2333)
[M::mem_pestat] skip orientation RR
[M::process] read 696772 sequences (100000277 bp) in 2.231 CPU sec, 2.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290560, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.87, 119.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696874 sequences (100000274 bp) in 1.390 CPU sec, 1.382 real sec...
[M::mem_process_seqs] Processed 697328 reads in 129.542 CPU sec, 4.114 real sec
[M::mem_process_seqs] Processed 697134 reads in 81.961 CPU sec, 2.599 real sec
sorting block 19, #reads = 1092455 mem = 536877977 peak = 1024880640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290329, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 216, 245)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495)
[M::mem_pestat] mean and std.dev: (170.11, 61.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 620)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.85, 118.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696424 sequences (100000273 bp) in 2.047 CPU sec, 2.186 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290423, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 231, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (221.33, 77.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.45, 119.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696772 reads in 127.323 CPU sec, 4.057 real sec
[M::process] read 700842 sequences (100000116 bp) in 1.490 CPU sec, 1.510 real sec...
[M::mem_process_seqs] Processed 696874 reads in 81.040 CPU sec, 2.554 real sec
sorting block 20, #reads = 1093334 mem = 536879884 peak = 1024880640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290206, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.42, 121.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702486 sequences (100000179 bp) in 2.193 CPU sec, 2.351 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291094, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (242.86, 115.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696424 reads in 129.214 CPU sec, 4.122 real sec
[M::mem_process_seqs] Processed 700842 reads in 83.398 CPU sec, 2.635 real sec
sorting block 21, #reads = 1091636 mem = 536884714 peak = 1024880640
[M::process] read 701012 sequences (100000138 bp) in 1.655 CPU sec, 1.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291841, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 267, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844)
[M::mem_pestat] mean and std.dev: (255.25, 123.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (238.22, 112.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 22, #reads = 1091168 mem = 536881333 peak = 1024880640
[M::process] read 700384 sequences (100000066 bp) in 1.976 CPU sec, 2.095 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291424, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (243.47, 115.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702486 reads in 129.196 CPU sec, 4.117 real sec
[M::process] read 700076 sequences (100000243 bp) in 1.435 CPU sec, 1.448 real sec...
[M::mem_process_seqs] Processed 701012 reads in 81.918 CPU sec, 2.589 real sec
sorting block 23, #reads = 1094575 mem = 536878203 peak = 1024880640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291373, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (246.14, 117.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699872 sequences (100000038 bp) in 2.236 CPU sec, 2.383 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291145, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.58, 118.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700384 reads in 127.112 CPU sec, 4.049 real sec
[M::mem_process_seqs] Processed 700076 reads in 81.431 CPU sec, 2.568 real sec
[M::process] read 699462 sequences (100000008 bp) in 1.609 CPU sec, 1.651 real sec...
sorting block 24, #reads = 1094782 mem = 536879741 peak = 1024880640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291453, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.07, 120.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 184, 205)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (97, 277)
[M::mem_pestat] mean and std.dev: (166.67, 32.92)
[M::mem_pestat] low and high boundaries for proper pairs: (35, 313)
[M::mem_pestat] skip orientation RR
[M::process] read 698550 sequences (100000160 bp) in 1.928 CPU sec, 2.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291409, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.11, 120.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699872 reads in 126.957 CPU sec, 4.045 real sec
[M::process] read 699556 sequences (100000100 bp) in 1.427 CPU sec, 1.448 real sec...
[M::mem_process_seqs] Processed 699462 reads in 80.873 CPU sec, 2.554 real sec
sorting block 25, #reads = 1094346 mem = 536876989 peak = 1024880640
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290651, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.50, 121.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697864 sequences (100000181 bp) in 2.197 CPU sec, 2.316 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291108, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 352, 699)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1613)
[M::mem_pestat] mean and std.dev: (467.27, 386.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2070)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.33, 119.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698550 reads in 127.792 CPU sec, 4.067 real sec
sorting block 26, #reads = 1094303 mem = 536876980 peak = 1024880640
[M::process] read 697292 sequences (100000095 bp) in 1.469 CPU sec, 1.483 real sec...
[M::mem_process_seqs] Processed 699556 reads in 81.316 CPU sec, 2.565 real sec
sorting block 27, #reads = 1093308 mem = 536881269 peak = 1040359424
[M::process] read 696954 sequences (100000197 bp) in 2.249 CPU sec, 2.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290807, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 277, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099)
[M::mem_pestat] mean and std.dev: (307.10, 140.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1413)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.21, 120.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 267, 1300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3570)
[M::mem_pestat] mean and std.dev: (592.70, 539.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4705)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697292 reads in 70.058 CPU sec, 2.211 real sec
[M::process] read 696920 sequences (100000233 bp) in 1.390 CPU sec, 1.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290671, 9, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.09, 121.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290386, 9, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (241, 419, 546)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1156)
[M::mem_pestat] mean and std.dev: (373.17, 155.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1461)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.46, 120.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697864 reads in 196.345 CPU sec, 6.244 real sec
sorting block 28, #reads = 1093114 mem = 536879296 peak = 1040359424
[M::process] read 697604 sequences (100000085 bp) in 1.691 CPU sec, 1.717 real sec...
[M::mem_process_seqs] Processed 696954 reads in 93.300 CPU sec, 2.962 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290507, 5, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 278, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (226.62, 83.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.70, 119.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 214, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462)
[M::mem_pestat] mean and std.dev: (180.33, 61.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 29, #reads = 1093453 mem = 536883167 peak = 1040359424
[M::process] read 697102 sequences (100000224 bp) in 2.315 CPU sec, 2.475 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290694, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.28, 118.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696920 reads in 128.600 CPU sec, 4.095 real sec
[M::mem_process_seqs] Processed 697604 reads in 82.754 CPU sec, 2.607 real sec
[M::process] read 696526 sequences (100000283 bp) in 1.536 CPU sec, 1.569 real sec...
sorting block 30, #reads = 1092352 mem = 536883859 peak = 1040359424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290099, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 309, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (240.80, 95.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.21, 119.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696660 sequences (100000159 bp) in 1.962 CPU sec, 2.122 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290152, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.64, 119.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697102 reads in 130.186 CPU sec, 4.147 real sec
[M::process] read 697286 sequences (100000255 bp) in 1.452 CPU sec, 1.458 real sec...
[M::mem_process_seqs] Processed 696526 reads in 83.216 CPU sec, 2.628 real sec
sorting block 31, #reads = 1091423 mem = 536878887 peak = 1040359424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290156, 3, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 197, 274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476)
[M::mem_pestat] mean and std.dev: (208.25, 48.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 577)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (254.75, 121.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 318, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160)
[M::mem_pestat] mean and std.dev: (322.22, 230.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1493)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 704118 sequences (100000173 bp) in 2.189 CPU sec, 2.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290177, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.47, 121.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701710 sequences (100000177 bp) in 1.370 CPU sec, 1.368 real sec...
[M::mem_process_seqs] Processed 696660 reads in 132.535 CPU sec, 4.212 real sec
sorting block 32, #reads = 1091108 mem = 536884951 peak = 1040359424
[M::mem_process_seqs] Processed 697286 reads in 85.886 CPU sec, 2.716 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292581, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603)
[M::mem_pestat] mean and std.dev: (233.31, 109.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 754)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1089687 mem = 536880375 peak = 1040359424
[M::process] read 700534 sequences (100000112 bp) in 1.982 CPU sec, 2.124 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291838, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 379, 760)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1796)
[M::mem_pestat] mean and std.dev: (483.91, 412.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2314)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.65, 115.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 704118 reads in 128.330 CPU sec, 4.088 real sec
[M::process] read 699718 sequences (100000073 bp) in 1.443 CPU sec, 1.450 real sec...
[M::mem_process_seqs] Processed 701710 reads in 80.964 CPU sec, 2.556 real sec
sorting block 34, #reads = 1096742 mem = 536879286 peak = 1040359424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291578, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.57, 117.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699144 sequences (100000216 bp) in 2.255 CPU sec, 2.400 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 291275, 0, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 395, 2361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6675)
[M::mem_pestat] mean and std.dev: (1057.94, 1357.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8832)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.95, 119.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 303, 959)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2353)
[M::mem_pestat] mean and std.dev: (479.50, 396.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3050)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700534 reads in 126.552 CPU sec, 4.026 real sec
[M::process] read 698998 sequences (100000257 bp) in 1.498 CPU sec, 1.526 real sec...
[M::mem_process_seqs] Processed 699718 reads in 80.492 CPU sec, 2.538 real sec
sorting block 35, #reads = 1096218 mem = 536876494 peak = 1040359424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291587, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 238, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (254.90, 119.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.15, 120.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698288 sequences (100000007 bp) in 1.898 CPU sec, 2.008 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291184, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.66, 121.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699144 reads in 126.117 CPU sec, 4.025 real sec
[M::process] read 698472 sequences (100000243 bp) in 1.505 CPU sec, 1.512 real sec...
[M::mem_process_seqs] Processed 698998 reads in 80.281 CPU sec, 2.532 real sec
sorting block 36, #reads = 1093916 mem = 536879225 peak = 1040359424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291082, 4, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 234, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (219.50, 84.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.68, 122.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698300 sequences (100000016 bp) in 1.989 CPU sec, 2.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290863, 2, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (279, 807, 3019)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8499)
[M::mem_pestat] mean and std.dev: (1854.55, 2315.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11239)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.05, 121.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698288 reads in 127.258 CPU sec, 4.056 real sec
[M::process] read 696906 sequences (100000089 bp) in 1.390 CPU sec, 1.388 real sec...
sorting block 37, #reads = 1095309 mem = 536879595 peak = 1040359424
[M::mem_process_seqs] Processed 698472 reads in 81.122 CPU sec, 2.571 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290786, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.88, 121.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1093385 mem = 536879397 peak = 1040359424
[M::process] read 696260 sequences (100000142 bp) in 2.029 CPU sec, 2.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290547, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.42, 121.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698300 reads in 127.196 CPU sec, 4.057 real sec
[M::mem_process_seqs] Processed 696906 reads in 80.967 CPU sec, 2.554 real sec
[M::process] read 696502 sequences (100000259 bp) in 1.546 CPU sec, 1.567 real sec...
sorting block 39, #reads = 1093084 mem = 536879415 peak = 1040359424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290308, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.65, 120.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (133, 178, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (225.23, 108.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RR
[M::process] read 696522 sequences (100000134 bp) in 2.022 CPU sec, 2.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290109, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.81, 118.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696260 reads in 128.412 CPU sec, 4.087 real sec
[M::process] read 696538 sequences (100000147 bp) in 1.432 CPU sec, 1.444 real sec...
[M::mem_process_seqs] Processed 696502 reads in 81.592 CPU sec, 2.575 real sec
sorting block 40, #reads = 1092455 mem = 536880450 peak = 1040359424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290126, 8, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.48, 119.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (342, 411, 766)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1614)
[M::mem_pestat] mean and std.dev: (433.00, 173.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2038)
[M::mem_pestat] skip orientation RR
[M::process] read 696498 sequences (100000020 bp) in 1.964 CPU sec, 2.066 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 289866, 10, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.32, 118.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (7, 564, 760)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2266)
[M::mem_pestat] mean and std.dev: (523.80, 468.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3019)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 696522 reads in 128.727 CPU sec, 4.103 real sec
[M::process] read 696304 sequences (100000209 bp) in 1.480 CPU sec, 1.490 real sec...
[M::mem_process_seqs] Processed 696538 reads in 81.470 CPU sec, 2.570 real sec
sorting block 41, #reads = 1092298 mem = 536879113 peak = 1040359424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290377, 5, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 323, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149)
[M::mem_pestat] mean and std.dev: (314.73, 231.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1485)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.44, 119.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 42, #reads = 1092128 mem = 536881806 peak = 1040359424
[M::process] read 696306 sequences (100000117 bp) in 2.002 CPU sec, 2.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 289979, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.17, 121.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696498 reads in 129.053 CPU sec, 4.109 real sec
[M::process] read 700614 sequences (100000276 bp) in 1.449 CPU sec, 1.451 real sec...
[M::mem_process_seqs] Processed 696304 reads in 82.426 CPU sec, 2.609 real sec
sorting block 43, #reads = 1091675 mem = 536880172 peak = 1040359424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290394, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 224, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (264.85, 171.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (255.72, 122.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 413, 1150)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3038)
[M::mem_pestat] mean and std.dev: (636.00, 663.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3982)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 705578 sequences (100000222 bp) in 2.037 CPU sec, 2.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291649, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (243.27, 116.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705294 sequences (100000264 bp) in 1.410 CPU sec, 1.412 real sec...
[M::mem_process_seqs] Processed 696306 reads in 134.060 CPU sec, 4.263 real sec
[M::mem_process_seqs] Processed 700614 reads in 87.395 CPU sec, 2.763 real sec
sorting block 44, #reads = 1090551 mem = 536880717 peak = 1040359424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292644, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 207, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (230.10, 108.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 737)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 518, 757)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1835)
[M::mem_pestat] mean and std.dev: (543.60, 422.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2374)
[M::mem_pestat] skip orientation RR
[M::process] read 704388 sequences (100000062 bp) in 2.299 CPU sec, 2.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293040, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (232.45, 111.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702720 sequences (100000123 bp) in 1.400 CPU sec, 1.402 real sec...
[M::mem_process_seqs] Processed 705578 reads in 138.439 CPU sec, 4.397 real sec
[M::mem_process_seqs] Processed 705294 reads in 85.598 CPU sec, 2.710 real sec
sorting block 45, #reads = 1090882 mem = 536878181 peak = 1040359424
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293068, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615)
[M::mem_pestat] mean and std.dev: (235.99, 113.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 770)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702020 sequences (100000274 bp) in 2.048 CPU sec, 2.166 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292548, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (241.21, 116.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704388 reads in 134.357 CPU sec, 4.279 real sec
[M::process] read 701612 sequences (100000171 bp) in 1.423 CPU sec, 1.433 real sec...
sorting block 46, #reads = 1094877 mem = 536877237 peak = 1040359424
[M::mem_process_seqs] Processed 702720 reads in 84.263 CPU sec, 2.663 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292189, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.26, 117.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1095931 mem = 536876960 peak = 1086861312
[M::process] read 701500 sequences (100000099 bp) in 2.333 CPU sec, 2.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292059, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (243.46, 118.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 308, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (96, 521)
[M::mem_pestat] mean and std.dev: (302.18, 80.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 625)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 702020 reads in 131.439 CPU sec, 4.170 real sec
[M::process] read 701536 sequences (100000116 bp) in 1.430 CPU sec, 1.431 real sec...
[M::mem_process_seqs] Processed 701612 reads in 82.556 CPU sec, 2.609 real sec
sorting block 48, #reads = 1094625 mem = 536881400 peak = 1086861312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291623, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (244.08, 118.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (236, 518, 1980)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5468)
[M::mem_pestat] mean and std.dev: (1093.82, 1387.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7212)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 701366 sequences (100000105 bp) in 1.994 CPU sec, 2.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291953, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 299, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (240.67, 97.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (243.93, 118.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 223, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700)
[M::mem_pestat] mean and std.dev: (253.64, 125.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 701500 reads in 131.505 CPU sec, 4.185 real sec
[M::process] read 700790 sequences (100000152 bp) in 1.440 CPU sec, 1.435 real sec...
[M::mem_process_seqs] Processed 701536 reads in 83.808 CPU sec, 2.646 real sec
sorting block 49, #reads = 1094781 mem = 536880179 peak = 1086861312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291958, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.79, 119.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700394 sequences (100000252 bp) in 2.260 CPU sec, 2.367 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292173, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.87, 119.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699536 sequences (100000068 bp) in 1.430 CPU sec, 1.433 real sec...
[M::mem_process_seqs] Processed 701366 reads in 133.242 CPU sec, 4.230 real sec
sorting block 50, #reads = 1092720 mem = 536880700 peak = 1086861312
[M::mem_process_seqs] Processed 700790 reads in 83.944 CPU sec, 2.654 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291893, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (247.76, 120.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 51, #reads = 1092968 mem = 536878376 peak = 1086861312
[M::process] read 698544 sequences (100000185 bp) in 2.076 CPU sec, 2.199 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291536, 5, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.97, 120.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 606, 1861)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5019)
[M::mem_pestat] mean and std.dev: (1000.31, 719.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6598)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700394 reads in 133.786 CPU sec, 4.265 real sec
[M::process] read 698016 sequences (100000150 bp) in 1.475 CPU sec, 1.478 real sec...
[M::mem_process_seqs] Processed 699536 reads in 85.755 CPU sec, 2.713 real sec
sorting block 52, #reads = 1093494 mem = 536879122 peak = 1086861312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290909, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.35, 119.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697506 sequences (100000026 bp) in 2.140 CPU sec, 2.273 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291044, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (177, 348, 885)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2301)
[M::mem_pestat] mean and std.dev: (574.77, 492.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3009)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.58, 120.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697694 sequences (100000059 bp) in 1.420 CPU sec, 1.411 real sec...
[M::mem_process_seqs] Processed 698544 reads in 135.227 CPU sec, 4.300 real sec
[M::mem_process_seqs] Processed 698016 reads in 85.915 CPU sec, 2.717 real sec
sorting block 53, #reads = 1092001 mem = 536878930 peak = 1086861312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290692, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.19, 121.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 203, 291)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545)
[M::mem_pestat] mean and std.dev: (176.00, 73.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 672)
[M::mem_pestat] skip orientation RR
[M::process] read 706500 sequences (100000100 bp) in 2.135 CPU sec, 2.252 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291010, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (253.06, 121.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697506 reads in 137.234 CPU sec, 4.367 real sec
[M::process] read 709618 sequences (100000298 bp) in 1.537 CPU sec, 1.536 real sec...
[M::mem_process_seqs] Processed 697694 reads in 87.329 CPU sec, 2.753 real sec
sorting block 54, #reads = 1090605 mem = 536878952 peak = 1086861312
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 293342, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (188, 275, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (231.10, 98.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 680)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589)
[M::mem_pestat] mean and std.dev: (229.06, 108.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 736)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 708690 sequences (100000270 bp) in 2.369 CPU sec, 2.479 real sec...
[M::process] read 706900 sequences (100000159 bp) in 1.470 CPU sec, 1.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 294391, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 199, 283)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (221.25, 102.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 700)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 706500 reads in 155.632 CPU sec, 4.949 real sec
sorting block 55, #reads = 1089665 mem = 536883951 peak = 1086861312
[M::mem_process_seqs] Processed 709618 reads in 99.860 CPU sec, 3.169 real sec
sorting block 56, #reads = 1088332 mem = 536879097 peak = 1103392768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293907, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (224.43, 105.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705096 sequences (100000030 bp) in 2.137 CPU sec, 2.255 real sec...
[M::process] read 703882 sequences (100000026 bp) in 1.460 CPU sec, 1.456 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293897, 9, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (229.55, 109.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 708690 reads in 152.138 CPU sec, 4.845 real sec
[M::mem_process_seqs] Processed 706900 reads in 96.360 CPU sec, 3.059 real sec
sorting block 57, #reads = 1089460 mem = 536877385 peak = 1103392768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293355, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (233.51, 111.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 757)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703324 sequences (100000299 bp) in 2.347 CPU sec, 2.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292392, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 211, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (236.02, 112.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702538 sequences (100000221 bp) in 1.450 CPU sec, 1.456 real sec...
[M::mem_process_seqs] Processed 705096 reads in 144.505 CPU sec, 4.589 real sec
sorting block 58, #reads = 1090187 mem = 536876467 peak = 1103392768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293190, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.37, 114.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701912 sequences (100000265 bp) in 1.619 CPU sec, 1.625 real sec...
[M::mem_process_seqs] Processed 703324 reads in 77.590 CPU sec, 2.464 real sec
[M::mem_process_seqs] Processed 703882 reads in 188.603 CPU sec, 5.991 real sec
sorting block 59, #reads = 1091460 mem = 536876688 peak = 1103392768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 292581, 7, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (274, 393, 580)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1192)
[M::mem_pestat] mean and std.dev: (417.09, 269.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1498)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (240.21, 115.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701480 sequences (100000297 bp) in 2.394 CPU sec, 2.536 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292200, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.99, 115.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702538 reads in 151.653 CPU sec, 4.812 real sec
[M::mem_process_seqs] Processed 701912 reads in 88.283 CPU sec, 2.787 real sec
[M::process] read 700810 sequences (100000260 bp) in 1.668 CPU sec, 1.720 real sec...
sorting block 60, #reads = 1091907 mem = 536878315 peak = 1103392768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292010, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.73, 117.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699736 sequences (100000250 bp) in 2.260 CPU sec, 2.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292126, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.66, 119.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698880 sequences (100000021 bp) in 1.370 CPU sec, 1.369 real sec...
[M::mem_process_seqs] Processed 701480 reads in 137.804 CPU sec, 4.381 real sec
[M::mem_process_seqs] Processed 700810 reads in 86.557 CPU sec, 2.736 real sec
sorting block 61, #reads = 1092220 mem = 536875904 peak = 1103392768
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291280, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.94, 120.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 62, #reads = 1092456 mem = 536882144 peak = 1126850560
[M::process] read 698842 sequences (100000110 bp) in 2.137 CPU sec, 2.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291502, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 239, 1344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3710)
[M::mem_pestat] mean and std.dev: (686.23, 769.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4893)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.32, 120.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698500 sequences (100000211 bp) in 1.420 CPU sec, 1.421 real sec...
[M::mem_process_seqs] Processed 699736 reads in 135.543 CPU sec, 4.305 real sec
[M::mem_process_seqs] Processed 698880 reads in 85.176 CPU sec, 2.694 real sec
sorting block 63, #reads = 1092113 mem = 536885926 peak = 1126850560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291338, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.39, 121.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699460 sequences (100000151 bp) in 2.334 CPU sec, 2.462 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291054, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 311, 1268)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3466)
[M::mem_pestat] mean and std.dev: (528.10, 488.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4565)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.72, 121.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (332, 502, 668)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1340)
[M::mem_pestat] mean and std.dev: (473.11, 191.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1676)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 705710 sequences (100000159 bp) in 1.400 CPU sec, 1.399 real sec...
[M::mem_process_seqs] Processed 698842 reads in 136.744 CPU sec, 4.339 real sec
[M::mem_process_seqs] Processed 698500 reads in 86.788 CPU sec, 2.744 real sec
sorting block 64, #reads = 1090555 mem = 536884025 peak = 1126850560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290730, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.13, 120.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704012 sequences (100000126 bp) in 2.348 CPU sec, 2.470 real sec...
[M::process] read 702804 sequences (100000084 bp) in 1.400 CPU sec, 1.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292904, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (273, 401, 595)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1239)
[M::mem_pestat] mean and std.dev: (420.00, 141.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1561)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592)
[M::mem_pestat] mean and std.dev: (229.87, 108.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 740)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699460 reads in 148.038 CPU sec, 4.706 real sec
[M::mem_process_seqs] Processed 705710 reads in 90.738 CPU sec, 2.875 real sec
sorting block 65, #reads = 1089695 mem = 536885179 peak = 1126850560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292624, 9, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (236.20, 112.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702152 sequences (100000052 bp) in 2.400 CPU sec, 2.527 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292404, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (238.35, 113.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701554 sequences (100000030 bp) in 1.390 CPU sec, 1.390 real sec...
[M::mem_process_seqs] Processed 704012 reads in 133.159 CPU sec, 4.225 real sec
sorting block 66, #reads = 1087738 mem = 536879089 peak = 1126850560
[M::mem_process_seqs] Processed 702804 reads in 83.830 CPU sec, 2.653 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292186, 4, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (240.93, 115.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 300, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (188, 388)
[M::mem_pestat] mean and std.dev: (287.78, 28.37)
[M::mem_pestat] low and high boundaries for proper pairs: (148, 428)
[M::mem_pestat] skip orientation RR
sorting block 67, #reads = 1094765 mem = 536878750 peak = 1129697280
[M::process] read 700584 sequences (100000146 bp) in 2.202 CPU sec, 2.327 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292395, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (242.64, 116.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702152 reads in 131.894 CPU sec, 4.204 real sec
[M::process] read 700578 sequences (100000105 bp) in 1.525 CPU sec, 1.550 real sec...
[M::mem_process_seqs] Processed 701554 reads in 84.585 CPU sec, 2.669 real sec
sorting block 68, #reads = 1095618 mem = 536878703 peak = 1129697280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291795, 7, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 390, 696)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1718)
[M::mem_pestat] mean and std.dev: (309.25, 179.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2229)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (244.54, 116.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701328 sequences (100000215 bp) in 1.962 CPU sec, 2.078 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292038, 9, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.43, 117.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 1327, 1327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3445)
[M::mem_pestat] mean and std.dev: (786.70, 543.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4504)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700584 reads in 129.392 CPU sec, 4.130 real sec
[M::process] read 701048 sequences (100000217 bp) in 1.496 CPU sec, 1.504 real sec...
[M::mem_process_seqs] Processed 700578 reads in 82.039 CPU sec, 2.588 real sec
sorting block 69, #reads = 1094341 mem = 536880020 peak = 1129697280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291943, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.10, 115.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699924 sequences (100000068 bp) in 2.180 CPU sec, 2.315 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291748, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (243.27, 116.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701328 reads in 131.746 CPU sec, 4.193 real sec
[M::process] read 699122 sequences (100000189 bp) in 1.517 CPU sec, 1.527 real sec...
[M::mem_process_seqs] Processed 701048 reads in 83.815 CPU sec, 2.649 real sec
sorting block 70, #reads = 1094720 mem = 536881447 peak = 1129697280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292064, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 261, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204)
[M::mem_pestat] mean and std.dev: (253.67, 145.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1560)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.42, 119.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 71, #reads = 1093828 mem = 536878964 peak = 1129697280
[M::process] read 698878 sequences (100000031 bp) in 2.370 CPU sec, 2.508 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291440, 5, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.60, 120.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 340, 811)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2061)
[M::mem_pestat] mean and std.dev: (465.86, 330.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2686)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699924 reads in 133.043 CPU sec, 4.231 real sec
[M::process] read 698948 sequences (100000089 bp) in 1.487 CPU sec, 1.502 real sec...
[M::mem_process_seqs] Processed 699122 reads in 84.276 CPU sec, 2.662 real sec
sorting block 72, #reads = 1093518 mem = 536881176 peak = 1129697280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291400, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 251, 251)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (155, 315)
[M::mem_pestat] mean and std.dev: (232.75, 27.63)
[M::mem_pestat] low and high boundaries for proper pairs: (122, 347)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.71, 120.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698416 sequences (100000064 bp) in 2.343 CPU sec, 2.486 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291260, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.72, 119.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 275, 567)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1221)
[M::mem_pestat] mean and std.dev: (325.83, 158.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1548)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698878 reads in 134.071 CPU sec, 4.262 real sec
[M::process] read 698256 sequences (100000141 bp) in 1.483 CPU sec, 1.488 real sec...
[M::mem_process_seqs] Processed 698948 reads in 85.801 CPU sec, 2.711 real sec
sorting block 73, #reads = 1092570 mem = 536881991 peak = 1129697280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290771, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.40, 119.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 160, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418)
[M::mem_pestat] mean and std.dev: (164.55, 49.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 505)
[M::mem_pestat] skip orientation RR
[M::process] read 698354 sequences (100000070 bp) in 2.320 CPU sec, 2.471 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290856, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 276, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (316.38, 157.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 948)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.12, 120.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700940 sequences (100000007 bp) in 1.440 CPU sec, 1.434 real sec...
[M::mem_process_seqs] Processed 698416 reads in 136.332 CPU sec, 4.327 real sec
[M::mem_process_seqs] Processed 698256 reads in 87.227 CPU sec, 2.757 real sec
sorting block 74, #reads = 1091435 mem = 536881415 peak = 1129697280
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290615, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.59, 120.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 287, 1928)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5338)
[M::mem_pestat] mean and std.dev: (471.90, 535.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7043)
[M::mem_pestat] skip orientation RR
[M::process] read 711534 sequences (100000298 bp) in 2.306 CPU sec, 2.391 real sec...
[M::process] read 706932 sequences (100000229 bp) in 1.460 CPU sec, 1.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291027, 7, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (242.48, 116.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 308, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875)
[M::mem_pestat] mean and std.dev: (289.20, 97.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1087)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698354 reads in 148.689 CPU sec, 4.721 real sec
sorting block 75, #reads = 1089877 mem = 536878342 peak = 1129697280
[M::mem_process_seqs] Processed 700940 reads in 99.872 CPU sec, 3.168 real sec
sorting block 76, #reads = 1084543 mem = 536884500 peak = 1177264128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 294795, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 196, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550)
[M::mem_pestat] mean and std.dev: (217.43, 101.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 686)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705406 sequences (100000145 bp) in 2.168 CPU sec, 2.292 real sec...
[M::process] read 703558 sequences (100000224 bp) in 1.420 CPU sec, 1.417 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 293533, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (229.47, 109.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 711534 reads in 155.869 CPU sec, 4.958 real sec
[M::mem_process_seqs] Processed 706932 reads in 98.025 CPU sec, 3.113 real sec
sorting block 77, #reads = 1085330 mem = 536881125 peak = 1177264128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293214, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (233.76, 112.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702086 sequences (100000008 bp) in 2.033 CPU sec, 2.125 real sec...
[M::process] read 701060 sequences (100000088 bp) in 1.440 CPU sec, 1.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292532, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (238.59, 114.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 705406 reads in 146.400 CPU sec, 4.657 real sec
[M::mem_process_seqs] Processed 703558 reads in 92.238 CPU sec, 2.925 real sec
sorting block 78, #reads = 1088618 mem = 536877379 peak = 1177264128
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292025, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.52, 117.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700908 sequences (100000130 bp) in 2.320 CPU sec, 2.434 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292109, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.30, 119.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699106 sequences (100000019 bp) in 1.430 CPU sec, 1.424 real sec...
[M::mem_process_seqs] Processed 702086 reads in 143.838 CPU sec, 4.570 real sec
sorting block 79, #reads = 1088749 mem = 536885384 peak = 1177264128
[M::mem_process_seqs] Processed 701060 reads in 90.992 CPU sec, 2.884 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291390, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (245.16, 118.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (100, 150, 201)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403)
[M::mem_pestat] mean and std.dev: (170.38, 83.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 504)
[M::mem_pestat] skip orientation RR
sorting block 80, #reads = 1088636 mem = 536879412 peak = 1184727040
[M::process] read 698956 sequences (100000097 bp) in 2.196 CPU sec, 2.323 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290883, 10, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.75, 121.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (52, 162, 3052)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9052)
[M::mem_pestat] mean and std.dev: (985.40, 1353.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 12052)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 698368 sequences (100000015 bp) in 1.430 CPU sec, 1.427 real sec...
[M::mem_process_seqs] Processed 700908 reads in 140.717 CPU sec, 4.475 real sec
[M::mem_process_seqs] Processed 699106 reads in 89.171 CPU sec, 2.823 real sec
sorting block 81, #reads = 1089122 mem = 536881052 peak = 1184727040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291367, 10, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 205, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557)
[M::mem_pestat] mean and std.dev: (196.88, 50.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 695)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (250.38, 121.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (205, 995, 1782)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4936)
[M::mem_pestat] mean and std.dev: (1249.40, 1224.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6513)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 698342 sequences (100000050 bp) in 2.078 CPU sec, 2.192 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290890, 5, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 460, 766)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1850)
[M::mem_pestat] mean and std.dev: (432.10, 256.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2392)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.72, 119.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 422, 889)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2373)
[M::mem_pestat] mean and std.dev: (374.60, 278.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3115)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698106 sequences (100000147 bp) in 1.390 CPU sec, 1.395 real sec...
[M::mem_process_seqs] Processed 698956 reads in 138.556 CPU sec, 4.412 real sec
[M::mem_process_seqs] Processed 698368 reads in 87.651 CPU sec, 2.772 real sec
sorting block 82, #reads = 1089276 mem = 536880392 peak = 1184727040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290294, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.69, 119.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697462 sequences (100000148 bp) in 2.143 CPU sec, 2.276 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290894, 10, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.58, 119.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (584, 1271, 8948)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25676)
[M::mem_pestat] mean and std.dev: (3148.20, 3818.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 34040)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 697244 sequences (100000188 bp) in 1.410 CPU sec, 1.410 real sec...
[M::mem_process_seqs] Processed 698342 reads in 136.496 CPU sec, 4.344 real sec
sorting block 83, #reads = 1089056 mem = 536878398 peak = 1184727040
[M::mem_process_seqs] Processed 698106 reads in 86.480 CPU sec, 2.734 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290474, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.17, 120.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 84, #reads = 1089892 mem = 536878528 peak = 1184727040
[M::process] read 697212 sequences (100000237 bp) in 2.274 CPU sec, 2.381 real sec...
[M::process] read 485734 sequences (69647198 bp) in 1.070 CPU sec, 1.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290581, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.90, 120.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697462 reads in 134.263 CPU sec, 4.275 real sec
[M::mem_process_seqs] Processed 697244 reads in 84.925 CPU sec, 2.698 real sec
sorting block 85, #reads = 1090174 mem = 536881880 peak = 1184727040
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290138, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.22, 120.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 202242, 10, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.13, 121.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (91, 747, 747)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2059)
[M::mem_pestat] mean and std.dev: (540.00, 317.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2715)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 697212 reads in 113.063 CPU sec, 3.675 real sec
[M::mem_process_seqs] Processed 485734 reads in 62.840 CPU sec, 2.037 real sec
sorting block 86, #reads = 1089715 mem = 536879092 peak = 1184727040
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 94987726
overall: 70079598592 mem 11125.373 user 314.058 sys 1108.169 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L005\tPU:L005\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1108.213 sec; CPU: 11439.438 sec
sorting block 87, #reads = 441260 mem = 217660421 peak = 1184727040
uniq header count 1
merging 88 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5271987435
output reads: 95472899
bam_mem_sort: 88 calls 30.806 user 0.165 sys 31.160 real
bam_write: 88 calls 52.794 user 1.166 sys 54.380 real
execute: 1 calls 17.336 user 0.902 sys 1136.059 real
merge_files: 1 calls 17.323 user 0.872 sys 27.234 real
parse_chunk: 1399 calls 43.502 user 11.925 sys 56.435 real
read_chunk: 3779 calls 38.048 user 32.840 sys 331.781 real
sort_block: 1 calls 29.108 user 32.260 sys 319.156 real
write_chunk: 2378 calls 17.002 user 0.651 sys 26.449 real
overall: 3090534400 mem 1056.272 user 54.672 sys 1136.084 real
ls: cannot access '*.cram': No such file or directory