Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/30/c5b4690a521cd5e4118963b29893ed/1136_5KC-T1-TDNA-1_A23MHWWLT4_1/1136_5KC-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cb/db66e177abafc4e1aeb34e801afb82/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/30/c5b4690a521cd5e4118963b29893ed/1136_5KC-T1-TDNA-1_A23MHWWLT4_1/1136_5KC-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/cb/db66e177abafc4e1aeb34e801afb82/.command.run ==> STAGING COMPLETE (7 inputs) cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_5KC-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1801.641 CPU sec, 65.545 real sec [M::process] read 690950 sequences (100000031 bp) in 1.191 CPU sec, 1.194 real sec... [M::process] read 690308 sequences (100000252 bp) in 1.806 CPU sec, 1.849 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286260, 2, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 226, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (293.26, 171.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 281, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (303.94, 151.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 273, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985) [M::mem_pestat] mean and std.dev: (266.03, 129.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1253) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690422 sequences (100000234 bp) in 1.659 CPU sec, 1.701 real sec... [M::process] read 689646 sequences (100000079 bp) in 1.547 CPU sec, 1.552 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 286111, 3, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 242, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (242.37, 104.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 283, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (305.73, 151.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (280, 376, 1243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3169) [M::mem_pestat] mean and std.dev: (617.74, 757.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4132) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690950 reads in 182.481 CPU sec, 5.823 real sec [M::mem_process_seqs] Processed 690308 reads in 103.229 CPU sec, 3.290 real sec sorting block 0, #reads = 1085207 mem = 536879678 peak = 597409792 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 285851, 14, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 228, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (260.10, 158.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 688872 sequences (100000058 bp) in 2.307 CPU sec, 2.339 real sec... [M::mem_pestat] (25, 50, 75) percentile: (187, 288, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (310.30, 154.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (364, 802, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1708) [M::mem_pestat] mean and std.dev: (678.46, 355.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2156) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 246, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (283.17, 126.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688580 sequences (100000051 bp) in 1.769 CPU sec, 1.774 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285861, 8, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 206, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (214.32, 96.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (306.69, 152.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 280, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (265.37, 116.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689646 reads in 163.173 CPU sec, 5.163 real sec [M::mem_process_seqs] Processed 690422 reads in 183.590 CPU sec, 5.832 real sec sorting block 1, #reads = 1084717 mem = 536879553 peak = 723238912 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285610, 7, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 275, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (270.62, 101.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 291, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (312.89, 155.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 294, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (302.37, 112.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687788 sequences (100000082 bp) in 2.293 CPU sec, 2.355 real sec... [M::process] read 687820 sequences (100000002 bp) in 1.821 CPU sec, 1.829 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 285828, 4, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 192, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (222.88, 122.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 295, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (316.45, 156.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 233, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (242.27, 101.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688872 reads in 164.529 CPU sec, 5.216 real sec sorting block 2, #reads = 1083943 mem = 536878705 peak = 839901184 [M::mem_process_seqs] Processed 688580 reads in 111.164 CPU sec, 3.543 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 285255, 4, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 195, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (197.85, 57.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 296, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (318.11, 157.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 325, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (347.65, 171.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687640 sequences (100000159 bp) in 2.673 CPU sec, 2.764 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 285052, 1, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 296, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (292.85, 131.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 299, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (320.42, 159.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 241, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (272.86, 126.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687490 sequences (100000051 bp) in 2.015 CPU sec, 2.015 real sec... [M::mem_process_seqs] Processed 687788 reads in 164.849 CPU sec, 5.215 real sec sorting block 3, #reads = 1084611 mem = 536881222 peak = 839901184 [M::mem_process_seqs] Processed 687820 reads in 112.716 CPU sec, 3.562 real sec sorting block 4, #reads = 1084028 mem = 536876380 peak = 858791936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 285615, 15, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 309, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (244.74, 93.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (320.87, 159.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (654, 665, 2092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4968) [M::mem_pestat] mean and std.dev: (1106.00, 745.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6406) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 259, 708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1788) [M::mem_pestat] mean and std.dev: (357.31, 340.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2328) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687576 sequences (100000190 bp) in 2.565 CPU sec, 2.686 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285219, 12, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 476, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172) [M::mem_pestat] mean and std.dev: (350.24, 163.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 297, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (319.29, 157.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (202, 1250, 6013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17635) [M::mem_pestat] mean and std.dev: (2116.83, 2468.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23446) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 248, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1105) [M::mem_pestat] mean and std.dev: (329.50, 210.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687242 sequences (100000256 bp) in 1.937 CPU sec, 1.939 real sec... [M::mem_process_seqs] Processed 687640 reads in 164.878 CPU sec, 5.217 real sec [M::mem_process_seqs] Processed 687490 reads in 112.558 CPU sec, 3.558 real sec sorting block 5, #reads = 1084273 mem = 536880968 peak = 858791936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 285169, 6, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 298, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2608) [M::mem_pestat] mean and std.dev: (510.27, 470.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 297, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (318.58, 157.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 247, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (257.07, 139.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687960 sequences (100000003 bp) in 2.775 CPU sec, 2.863 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284920, 9, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 231, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200) [M::mem_pestat] mean and std.dev: (296.89, 234.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 296, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (317.51, 156.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 259, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063) [M::mem_pestat] mean and std.dev: (308.64, 201.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1368) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687654 sequences (100000106 bp) in 2.252 CPU sec, 2.271 real sec... [M::mem_process_seqs] Processed 687576 reads in 168.440 CPU sec, 5.320 real sec [M::mem_process_seqs] Processed 687242 reads in 113.193 CPU sec, 3.585 real sec sorting block 6, #reads = 1083054 mem = 536877996 peak = 858791936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 285002, 10, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 255, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (241.41, 89.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 294, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (316.36, 156.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (666, 1617, 2559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6345) [M::mem_pestat] mean and std.dev: (2269.90, 2192.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11040) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 370, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003) [M::mem_pestat] mean and std.dev: (367.40, 181.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1253) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687468 sequences (100000183 bp) in 2.322 CPU sec, 2.413 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 285023, 12, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 246, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (252.02, 143.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 295, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (316.77, 156.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (585, 1057, 1246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2568) [M::mem_pestat] mean and std.dev: (925.75, 351.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3229) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 268, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (268.35, 101.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687002 sequences (100000160 bp) in 1.937 CPU sec, 1.940 real sec... [M::mem_process_seqs] Processed 687960 reads in 168.543 CPU sec, 5.348 real sec sorting block 7, #reads = 1084196 mem = 536880890 peak = 858791936 [M::mem_process_seqs] Processed 687654 reads in 113.549 CPU sec, 3.606 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 284594, 8, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 229, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (253.91, 107.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 295, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (318.04, 157.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 252, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (251.54, 92.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686924 sequences (100000070 bp) in 2.651 CPU sec, 2.724 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284705, 7, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 221, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (260.70, 138.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 297, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (319.06, 157.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 181, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (203.88, 106.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686750 sequences (100000251 bp) in 2.140 CPU sec, 2.138 real sec... [M::mem_process_seqs] Processed 687468 reads in 168.516 CPU sec, 5.331 real sec sorting block 8, #reads = 1083828 mem = 536876839 peak = 858791936 [M::mem_process_seqs] Processed 687002 reads in 113.835 CPU sec, 3.594 real sec sorting block 9, #reads = 1083517 mem = 536879715 peak = 914599936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 285424, 5, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 239, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (240.31, 87.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 298, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (319.25, 156.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 247, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (307.22, 155.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686126 sequences (100000225 bp) in 2.511 CPU sec, 2.585 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 284217, 4, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 308, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (313.34, 141.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (320.21, 156.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 370, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032) [M::mem_pestat] mean and std.dev: (374.07, 166.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686218 sequences (100000136 bp) in 2.055 CPU sec, 2.058 real sec... [M::mem_process_seqs] Processed 686924 reads in 164.261 CPU sec, 5.203 real sec [M::mem_process_seqs] Processed 686750 reads in 112.786 CPU sec, 3.576 real sec sorting block 10, #reads = 1083899 mem = 536877795 peak = 914599936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284817, 5, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 248, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (236.85, 106.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 302, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (322.07, 156.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 216, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (255.62, 121.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685912 sequences (100000202 bp) in 2.984 CPU sec, 3.069 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285049, 6, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 354, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1166) [M::mem_pestat] mean and std.dev: (307.65, 189.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (197, 302, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (321.50, 156.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 241, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1524) [M::mem_pestat] mean and std.dev: (318.15, 237.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1976) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686126 reads in 165.923 CPU sec, 5.247 real sec [M::process] read 685610 sequences (100000135 bp) in 2.260 CPU sec, 2.275 real sec... [M::mem_process_seqs] Processed 686218 reads in 112.902 CPU sec, 3.562 real sec sorting block 11, #reads = 1082828 mem = 536882602 peak = 914599936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 284440, 4, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 181, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (244.41, 148.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (198, 304, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (324.04, 157.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 228, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (268.54, 124.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 685206 sequences (100000012 bp) in 2.677 CPU sec, 2.775 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 284580, 11, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 227, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (250.24, 130.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (200, 307, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (326.93, 159.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (963, 1293, 1528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2658) [M::mem_pestat] mean and std.dev: (1139.33, 386.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 299, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (284.80, 117.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685912 reads in 164.714 CPU sec, 5.225 real sec [M::mem_process_seqs] Processed 685610 reads in 114.500 CPU sec, 3.599 real sec [M::process] read 688214 sequences (100000152 bp) in 2.488 CPU sec, 2.513 real sec... sorting block 12, #reads = 1083642 mem = 536879473 peak = 914599936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 283964, 8, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 234, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (283.26, 151.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (201, 309, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (328.35, 159.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 314, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1337) [M::mem_pestat] mean and std.dev: (328.84, 221.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1715) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 13, #reads = 1083585 mem = 536878832 peak = 914599936 [M::process] read 690802 sequences (100000233 bp) in 2.906 CPU sec, 2.989 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 284692, 15, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 222, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (291.97, 197.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 295, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (316.12, 156.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1037, 1279, 3055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7091) [M::mem_pestat] mean and std.dev: (1852.40, 1181.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9109) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 294, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1287) [M::mem_pestat] mean and std.dev: (347.76, 264.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 685206 reads in 169.587 CPU sec, 5.376 real sec [M::process] read 690436 sequences (100000249 bp) in 2.458 CPU sec, 2.479 real sec... [M::mem_process_seqs] Processed 688214 reads in 119.255 CPU sec, 3.751 real sec sorting block 14, #reads = 1080982 mem = 536879503 peak = 914599936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 285724, 2, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 181, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (253.91, 138.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (307.61, 154.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 298, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063) [M::mem_pestat] mean and std.dev: (313.98, 156.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1344) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689946 sequences (100000134 bp) in 2.637 CPU sec, 2.717 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 286337, 10, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 366, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (320.21, 181.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 288, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (309.87, 154.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (700, 2786, 2786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6958) [M::mem_pestat] mean and std.dev: (2095.40, 1368.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9044) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 192, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (212.91, 77.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690802 reads in 165.789 CPU sec, 5.267 real sec [M::mem_process_seqs] Processed 690436 reads in 113.085 CPU sec, 3.554 real sec [M::process] read 689608 sequences (100000106 bp) in 2.550 CPU sec, 2.576 real sec... sorting block 15, #reads = 1087430 mem = 536879076 peak = 914599936 [M::process] read 688976 sequences (100000044 bp) in 2.607 CPU sec, 2.704 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285686, 4, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 302, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (269.49, 117.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 291, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (313.10, 156.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 260, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (312.58, 208.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1306) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689608 reads in 94.277 CPU sec, 2.964 real sec [M::process] read 688532 sequences (100000037 bp) in 2.296 CPU sec, 2.310 real sec... sorting block 16, #reads = 1087017 mem = 536881339 peak = 914599936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285427, 6, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (113, 209, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (211.69, 107.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 289, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (311.26, 155.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 253, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2947) [M::mem_pestat] mean and std.dev: (741.03, 893.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 285392, 4, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 219, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (230.43, 79.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 294, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (315.98, 157.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 267, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (280.61, 154.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689946 reads in 259.454 CPU sec, 8.225 real sec [M::mem_process_seqs] Processed 688976 reads in 132.244 CPU sec, 4.170 real sec [M::process] read 688122 sequences (100000119 bp) in 2.565 CPU sec, 2.605 real sec... sorting block 17, #reads = 1085665 mem = 536878659 peak = 914599936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 285176, 9, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 284, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (282.03, 121.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 297, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (318.19, 158.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 261, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (226.24, 76.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687974 sequences (100000242 bp) in 2.665 CPU sec, 2.743 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285144, 8, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 276, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1273) [M::mem_pestat] mean and std.dev: (295.42, 206.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (319.56, 158.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 226, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (204.35, 56.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688532 reads in 163.130 CPU sec, 5.172 real sec [M::mem_process_seqs] Processed 688122 reads in 113.589 CPU sec, 3.573 real sec [M::process] read 688068 sequences (100000054 bp) in 2.598 CPU sec, 2.641 real sec... sorting block 18, #reads = 1085243 mem = 536879514 peak = 914599936 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285602, 12, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 244, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (263.39, 122.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 301, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (321.73, 159.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (615, 5285, 5285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14625) [M::mem_pestat] mean and std.dev: (3280.92, 2272.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19295) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 295, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 538) [M::mem_pestat] mean and std.dev: (285.11, 100.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687546 sequences (100000266 bp) in 2.979 CPU sec, 3.055 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 284912, 5, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (118, 201, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (226.11, 115.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (321.50, 160.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 283, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1621) [M::mem_pestat] mean and std.dev: (461.69, 370.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687974 reads in 165.892 CPU sec, 5.261 real sec [M::process] read 688116 sequences (100000124 bp) in 2.115 CPU sec, 2.130 real sec... sorting block 19, #reads = 1085213 mem = 536878554 peak = 914599936 [M::mem_process_seqs] Processed 688068 reads in 114.918 CPU sec, 3.620 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284899, 13, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 218, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (253.61, 143.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1255) [M::mem_pestat] analyzing insert size distribution for orientation FR... sorting block 20, #reads = 1084900 mem = 536875271 peak = 914853888 [M::mem_pestat] (25, 50, 75) percentile: (193, 299, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (321.89, 160.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (377, 709, 2622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7112) [M::mem_pestat] mean and std.dev: (1102.17, 917.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9357) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 332, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (271.28, 120.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687746 sequences (100000235 bp) in 2.730 CPU sec, 2.816 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 285393, 5, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 286, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1128) [M::mem_pestat] mean and std.dev: (289.70, 214.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 297, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (320.33, 160.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 295, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (364.26, 228.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1280) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687546 reads in 166.987 CPU sec, 5.283 real sec [M::process] read 688108 sequences (100000183 bp) in 2.360 CPU sec, 2.373 real sec... [M::mem_process_seqs] Processed 688116 reads in 113.315 CPU sec, 3.561 real sec sorting block 21, #reads = 1085099 mem = 536879523 peak = 914853888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 284524, 6, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 220, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003) [M::mem_pestat] mean and std.dev: (254.58, 147.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 296, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (318.44, 157.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 235, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (277.22, 146.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687982 sequences (100000054 bp) in 2.991 CPU sec, 3.062 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 284987, 6, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 299, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136) [M::mem_pestat] mean and std.dev: (373.77, 194.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 292, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (314.56, 155.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 261, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978) [M::mem_pestat] mean and std.dev: (281.37, 138.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1234) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687746 reads in 165.335 CPU sec, 5.233 real sec [M::mem_process_seqs] Processed 688108 reads in 113.548 CPU sec, 3.570 real sec [M::process] read 688028 sequences (100000233 bp) in 2.456 CPU sec, 2.491 real sec... sorting block 22, #reads = 1084731 mem = 536880116 peak = 914853888 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 285303, 9, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 254, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079) [M::mem_pestat] mean and std.dev: (242.70, 106.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 292, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (314.37, 155.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 282, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (309.04, 141.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687496 sequences (100000009 bp) in 2.147 CPU sec, 2.234 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 285319, 10, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 206, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (214.94, 68.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 294, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (315.06, 155.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (811, 1100, 2893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7057) [M::mem_pestat] mean and std.dev: (1425.40, 1085.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9139) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 267, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244) [M::mem_pestat] mean and std.dev: (338.77, 249.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687354 sequences (100000165 bp) in 1.640 CPU sec, 1.635 real sec... [M::mem_process_seqs] Processed 687982 reads in 164.807 CPU sec, 5.250 real sec sorting block 23, #reads = 1084232 mem = 536879986 peak = 979701760 [M::mem_process_seqs] Processed 688028 reads in 112.230 CPU sec, 3.562 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 285395, 8, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 296, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (234.62, 111.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 295, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 865) [M::mem_pestat] mean and std.dev: (316.16, 155.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 242, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (266.90, 157.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687002 sequences (100000194 bp) in 2.989 CPU sec, 3.053 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284846, 7, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 314, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (237.33, 99.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 295, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (315.47, 154.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 213, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (225.29, 79.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687496 reads in 162.562 CPU sec, 5.136 real sec sorting block 24, #reads = 1084742 mem = 536879117 peak = 979701760 [M::process] read 686566 sequences (100000079 bp) in 2.387 CPU sec, 2.401 real sec... [M::mem_process_seqs] Processed 687354 reads in 111.027 CPU sec, 3.490 real sec sorting block 25, #reads = 1083410 mem = 536877155 peak = 979701760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 285050, 7, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 240, 855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2181) [M::mem_pestat] mean and std.dev: (439.93, 489.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 298, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (319.25, 157.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 251, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1541) [M::mem_pestat] mean and std.dev: (406.90, 325.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1986) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686654 sequences (100000222 bp) in 2.279 CPU sec, 2.363 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 284997, 7, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 187, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (235.23, 128.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1224) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (321.50, 158.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 209, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (239.65, 89.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687002 reads in 163.990 CPU sec, 5.191 real sec [M::process] read 686306 sequences (100000251 bp) in 2.250 CPU sec, 2.251 real sec... [M::mem_process_seqs] Processed 686566 reads in 112.413 CPU sec, 3.542 real sec sorting block 26, #reads = 1084221 mem = 536881291 peak = 979701760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284802, 6, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 269, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (264.30, 120.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (321.27, 157.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 293, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (289.07, 140.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1208) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 686630 sequences (100000218 bp) in 3.011 CPU sec, 3.089 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 284622, 7, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 264, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (251.02, 114.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 302, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (322.76, 158.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 336, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1416) [M::mem_pestat] mean and std.dev: (323.32, 161.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1816) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686654 reads in 162.931 CPU sec, 5.159 real sec [M::mem_process_seqs] Processed 686306 reads in 112.113 CPU sec, 3.523 real sec [M::process] read 686664 sequences (100000282 bp) in 2.445 CPU sec, 2.473 real sec... sorting block 27, #reads = 1083448 mem = 536879381 peak = 979701760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 284591, 12, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 269, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031) [M::mem_pestat] mean and std.dev: (339.73, 186.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (324.67, 161.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (479, 671, 677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (83, 1073) [M::mem_pestat] mean and std.dev: (611.91, 173.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 257, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165) [M::mem_pestat] mean and std.dev: (299.50, 167.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1495) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692198 sequences (100000140 bp) in 2.681 CPU sec, 2.766 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 284616, 5, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 222, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101) [M::mem_pestat] mean and std.dev: (242.97, 154.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (196, 303, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (325.98, 162.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 275, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (294.56, 171.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1260) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686630 reads in 164.501 CPU sec, 5.237 real sec [M::mem_process_seqs] Processed 686664 reads in 113.975 CPU sec, 3.586 real sec [M::process] read 691970 sequences (100000152 bp) in 2.398 CPU sec, 2.441 real sec... sorting block 28, #reads = 1082844 mem = 536877527 peak = 979701760 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 286385, 7, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 233, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (239.50, 115.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 280, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (302.50, 151.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 238, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (278.83, 125.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691664 sequences (100000032 bp) in 2.583 CPU sec, 2.661 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 286069, 7, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 259, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (275.81, 134.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 281, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (303.97, 152.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 245, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (287.19, 143.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692198 reads in 167.128 CPU sec, 5.296 real sec sorting block 29, #reads = 1081361 mem = 536880168 peak = 979701760 [M::process] read 690686 sequences (100000202 bp) in 2.189 CPU sec, 2.195 real sec... [M::mem_process_seqs] Processed 691970 reads in 113.294 CPU sec, 3.573 real sec sorting block 30, #reads = 1086917 mem = 536878273 peak = 1042194432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 286022, 9, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 227, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046) [M::mem_pestat] mean and std.dev: (310.24, 220.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 282, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (304.22, 151.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 191, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (231.84, 123.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689866 sequences (100000171 bp) in 2.739 CPU sec, 2.808 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 286194, 5, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 271, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (279.61, 174.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 285, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (306.12, 151.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 234, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (252.10, 112.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691664 reads in 166.076 CPU sec, 5.258 real sec [M::process] read 689122 sequences (100000290 bp) in 2.427 CPU sec, 2.448 real sec... [M::mem_process_seqs] Processed 690686 reads in 113.339 CPU sec, 3.564 real sec sorting block 31, #reads = 1086833 mem = 536878075 peak = 1042194432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 285894, 8, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 216, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1141) [M::mem_pestat] mean and std.dev: (310.30, 194.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 289, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (309.15, 151.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 273, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (293.70, 121.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688682 sequences (100000079 bp) in 2.534 CPU sec, 2.619 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 285799, 16, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 281, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (239.42, 78.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 293, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (313.25, 153.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (452, 777, 1971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5009) [M::mem_pestat] mean and std.dev: (901.00, 780.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6528) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 227, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (242.40, 106.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689866 reads in 167.097 CPU sec, 5.315 real sec [M::process] read 688644 sequences (100000240 bp) in 2.282 CPU sec, 2.294 real sec... [M::mem_process_seqs] Processed 689122 reads in 113.436 CPU sec, 3.578 real sec sorting block 32, #reads = 1085188 mem = 536877271 peak = 1042194432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 285176, 8, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 252, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (300.77, 176.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1205) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 294, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (313.34, 152.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 197, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (203.92, 89.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688012 sequences (100000001 bp) in 2.578 CPU sec, 2.676 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 285267, 2, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 212, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (287.90, 193.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 296, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (315.94, 155.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 314, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (300.86, 109.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688082 sequences (100000011 bp) in 1.847 CPU sec, 1.853 real sec... [M::mem_process_seqs] Processed 688682 reads in 168.675 CPU sec, 5.357 real sec sorting block 33, #reads = 1085314 mem = 536879464 peak = 1042194432 [M::mem_process_seqs] Processed 688644 reads in 115.302 CPU sec, 3.651 real sec sorting block 34, #reads = 1084615 mem = 536879448 peak = 1042194432 [M::process] read 687550 sequences (100000220 bp) in 2.944 CPU sec, 3.026 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285055, 14, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 183, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (262.97, 156.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (317.76, 155.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (7, 567, 1687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5047) [M::mem_pestat] mean and std.dev: (1093.57, 1247.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6727) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 227, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (217.10, 76.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688082 reads in 97.050 CPU sec, 3.050 real sec [M::process] read 688402 sequences (100000289 bp) in 2.432 CPU sec, 2.449 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 284992, 8, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 209, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (242.77, 124.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 297, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (316.65, 154.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 251, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (281.39, 133.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 285499, 4, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (140, 182, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (204.33, 86.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 299, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (319.15, 156.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 229, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (249.15, 111.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1256) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688012 reads in 264.550 CPU sec, 8.384 real sec [M::mem_process_seqs] Processed 687550 reads in 137.811 CPU sec, 4.358 real sec sorting block 35, #reads = 1085217 mem = 536880609 peak = 1042194432 [M::process] read 687684 sequences (100000252 bp) in 2.504 CPU sec, 2.531 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 285243, 15, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 216, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (241.15, 108.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 294, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (315.00, 155.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1807, 1827, 2118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1185, 2740) [M::mem_pestat] mean and std.dev: (1872.56, 101.65) [M::mem_pestat] low and high boundaries for proper pairs: (874, 3051) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 250, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (272.73, 132.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 36, #reads = 1083962 mem = 536877951 peak = 1042194432 [M::process] read 687612 sequences (100000242 bp) in 2.776 CPU sec, 2.871 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 284954, 12, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 285, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (305.50, 202.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 295, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (315.88, 155.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (359, 359, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139) [M::mem_pestat] mean and std.dev: (404.42, 166.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1399) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 276, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (268.34, 96.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688402 reads in 169.977 CPU sec, 5.407 real sec [M::mem_process_seqs] Processed 687684 reads in 117.464 CPU sec, 3.693 real sec [M::process] read 687926 sequences (100000195 bp) in 2.570 CPU sec, 2.631 real sec... sorting block 37, #reads = 1084931 mem = 536880550 peak = 1042194432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 285098, 8, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 1655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4597) [M::mem_pestat] mean and std.dev: (886.52, 1249.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6068) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 294, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (314.88, 154.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 343, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2924) [M::mem_pestat] mean and std.dev: (498.57, 440.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3829) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687428 sequences (100000006 bp) in 2.953 CPU sec, 3.050 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 285009, 8, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 293, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167) [M::mem_pestat] mean and std.dev: (301.47, 152.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 293, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (314.37, 154.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 241, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (277.69, 134.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687612 reads in 170.920 CPU sec, 5.420 real sec [M::process] read 687486 sequences (100000036 bp) in 2.356 CPU sec, 2.374 real sec... [M::mem_process_seqs] Processed 687926 reads in 116.653 CPU sec, 3.668 real sec sorting block 38, #reads = 1083172 mem = 536879542 peak = 1042194432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 285057, 2, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 275, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (250.71, 128.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 296, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (317.25, 155.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 263, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (280.00, 144.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687198 sequences (100000183 bp) in 2.717 CPU sec, 2.791 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285024, 7, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 218, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (210.44, 78.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 295, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (315.33, 153.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 245, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (266.91, 118.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687428 reads in 171.342 CPU sec, 5.433 real sec [M::process] read 687348 sequences (100000203 bp) in 2.236 CPU sec, 2.250 real sec... [M::mem_process_seqs] Processed 687486 reads in 118.460 CPU sec, 3.731 real sec sorting block 39, #reads = 1083061 mem = 536879708 peak = 1042194432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 284707, 10, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 288, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1151) [M::mem_pestat] mean and std.dev: (286.84, 195.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 295, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (315.89, 153.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (143, 961, 4425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12989) [M::mem_pestat] mean and std.dev: (1968.40, 2150.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17271) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (268, 336, 1094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2746) [M::mem_pestat] mean and std.dev: (537.27, 469.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3572) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687220 sequences (100000009 bp) in 2.362 CPU sec, 2.474 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 284965, 11, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 184, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (226.80, 104.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (318.47, 156.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (542, 903, 1235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2621) [M::mem_pestat] mean and std.dev: (644.44, 359.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3314) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 399, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1685) [M::mem_pestat] mean and std.dev: (456.81, 312.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2160) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686912 sequences (100000075 bp) in 2.080 CPU sec, 2.082 real sec... [M::mem_process_seqs] Processed 687198 reads in 171.264 CPU sec, 5.439 real sec sorting block 40, #reads = 1082585 mem = 536881730 peak = 1042194432 [M::mem_process_seqs] Processed 687348 reads in 116.240 CPU sec, 3.680 real sec sorting block 41, #reads = 1082789 mem = 536879868 peak = 1042194432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 285249, 6, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 229, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (282.91, 166.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (319.33, 157.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 280, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (278.10, 128.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687142 sequences (100000217 bp) in 2.491 CPU sec, 2.558 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285507, 6, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 263, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (235.39, 88.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 298, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (319.84, 157.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 285, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (254.11, 121.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687160 sequences (100000213 bp) in 1.900 CPU sec, 1.902 real sec... [M::mem_process_seqs] Processed 687220 reads in 170.351 CPU sec, 5.401 real sec [M::mem_process_seqs] Processed 686912 reads in 113.957 CPU sec, 3.608 real sec sorting block 42, #reads = 1081921 mem = 536880112 peak = 1042194432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 285125, 2, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 291, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (242.20, 103.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (320.72, 159.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 322, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072) [M::mem_pestat] mean and std.dev: (295.84, 177.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1378) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690808 sequences (100000239 bp) in 2.977 CPU sec, 3.052 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285578, 19, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 226, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (220.39, 99.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 299, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (321.01, 159.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (94, 1273, 2305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6727) [M::mem_pestat] mean and std.dev: (1669.47, 1715.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8938) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 283, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (288.16, 153.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687142 reads in 170.759 CPU sec, 5.385 real sec [M::process] read 690692 sequences (100000106 bp) in 2.254 CPU sec, 2.255 real sec... [M::mem_process_seqs] Processed 687160 reads in 118.322 CPU sec, 3.725 real sec sorting block 43, #reads = 1082835 mem = 536880287 peak = 1042194432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 286153, 8, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 276, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (283.28, 138.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 284, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (305.51, 151.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 276, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (259.07, 91.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690514 sequences (100000182 bp) in 2.669 CPU sec, 2.755 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 285510, 9, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 354, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (281.53, 117.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 285, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (306.83, 152.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 278, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (271.74, 142.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690808 reads in 169.737 CPU sec, 5.373 real sec [M::process] read 690224 sequences (100000290 bp) in 2.216 CPU sec, 2.219 real sec... sorting block 44, #reads = 1082003 mem = 536879024 peak = 1042194432 [M::mem_process_seqs] Processed 690692 reads in 114.086 CPU sec, 3.604 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 285643, 10, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 215, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (233.30, 135.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 286, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (307.63, 152.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 1885, 1953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5425) [M::mem_pestat] mean and std.dev: (1302.90, 1136.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7161) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 299, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (287.46, 159.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689836 sequences (100000115 bp) in 2.860 CPU sec, 2.949 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 285776, 5, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 282, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (282.23, 120.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 289, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (310.32, 153.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 247, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (268.25, 125.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690514 reads in 168.359 CPU sec, 5.335 real sec sorting block 45, #reads = 1086799 mem = 536880311 peak = 1042194432 [M::process] read 689586 sequences (100000003 bp) in 2.351 CPU sec, 2.356 real sec... [M::mem_process_seqs] Processed 690224 reads in 114.176 CPU sec, 3.588 real sec sorting block 46, #reads = 1087002 mem = 536878814 peak = 1042194432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 285644, 1, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 221, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (207.25, 90.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 291, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (311.31, 154.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 288, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146) [M::mem_pestat] mean and std.dev: (333.56, 181.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1460) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688574 sequences (100000021 bp) in 2.314 CPU sec, 2.393 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 285234, 8, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 301, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (374.20, 255.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1395) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 688452 sequences (100000144 bp) in 1.680 CPU sec, 1.684 real sec... [M::mem_pestat] (25, 50, 75) percentile: (188, 291, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (312.47, 154.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 292, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (291.68, 113.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689836 reads in 166.250 CPU sec, 5.280 real sec [M::mem_process_seqs] Processed 689586 reads in 113.628 CPU sec, 3.600 real sec sorting block 47, #reads = 1086144 mem = 536878579 peak = 1042194432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 285463, 5, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 434, 1117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2937) [M::mem_pestat] mean and std.dev: (767.35, 894.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 297, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (316.97, 156.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 306, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146) [M::mem_pestat] mean and std.dev: (347.46, 196.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1454) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688596 sequences (100000207 bp) in 2.861 CPU sec, 2.940 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 285288, 11, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 222, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (204.18, 72.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 296, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (315.33, 154.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (314, 505, 595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1157) [M::mem_pestat] mean and std.dev: (371.80, 218.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1438) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 188, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (242.78, 121.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688238 sequences (100000234 bp) in 2.052 CPU sec, 2.052 real sec... [M::mem_process_seqs] Processed 688574 reads in 169.933 CPU sec, 5.369 real sec [M::mem_process_seqs] Processed 688452 reads in 117.046 CPU sec, 3.693 real sec sorting block 48, #reads = 1084405 mem = 536878350 peak = 1042194432 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 285185, 10, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 153, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (220.68, 121.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 297, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (316.57, 155.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (7, 959, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2863) [M::mem_pestat] mean and std.dev: (507.22, 513.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3815) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 292, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (266.66, 107.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688378 sequences (100000278 bp) in 2.791 CPU sec, 2.877 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 284592, 10, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 368, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (302.13, 148.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 298, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (317.60, 155.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (94, 858, 858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2386) [M::mem_pestat] mean and std.dev: (632.60, 508.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 254, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (286.10, 156.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688596 reads in 169.553 CPU sec, 5.360 real sec [M::process] read 689288 sequences (100000287 bp) in 2.267 CPU sec, 2.268 real sec... sorting block 49, #reads = 1084812 mem = 536878612 peak = 1042194432 [M::mem_process_seqs] Processed 688238 reads in 116.222 CPU sec, 3.658 real sec sorting block 50, #reads = 1084193 mem = 536881245 peak = 1061593088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 285521, 6, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 199, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (199.18, 89.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 297, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (316.94, 155.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 261, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (270.94, 99.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689290 sequences (100000231 bp) in 2.773 CPU sec, 2.852 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 285895, 9, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 189, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (212.33, 97.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 289, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (312.71, 155.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 292, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (272.28, 91.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688772 sequences (100000165 bp) in 1.826 CPU sec, 1.835 real sec... [M::mem_process_seqs] Processed 688378 reads in 172.066 CPU sec, 5.456 real sec [M::mem_process_seqs] Processed 689288 reads in 116.980 CPU sec, 3.708 real sec sorting block 51, #reads = 1083310 mem = 536878681 peak = 1061593088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284999, 6, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 224, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (251.70, 113.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 287, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (308.81, 152.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 330, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1270) [M::mem_pestat] mean and std.dev: (419.08, 244.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688122 sequences (100000171 bp) in 2.809 CPU sec, 2.888 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 285359, 15, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 316, 711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1781) [M::mem_pestat] mean and std.dev: (424.45, 289.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 290, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (311.48, 153.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (137, 810, 9856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 29294) [M::mem_pestat] mean and std.dev: (3110.53, 4106.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 39013) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 233, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (267.75, 163.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689290 reads in 173.085 CPU sec, 5.487 real sec [M::process] read 688002 sequences (100000118 bp) in 2.319 CPU sec, 2.330 real sec... [M::mem_process_seqs] Processed 688772 reads in 118.747 CPU sec, 3.746 real sec sorting block 52, #reads = 1083823 mem = 536879257 peak = 1061593088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285635, 4, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 262, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1144) [M::mem_pestat] mean and std.dev: (292.98, 165.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 291, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (311.51, 151.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 239, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (224.68, 91.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688260 sequences (100000115 bp) in 2.751 CPU sec, 2.816 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285554, 6, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 268, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (308.97, 185.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 291, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (310.92, 150.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 248, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (303.93, 191.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688122 reads in 171.259 CPU sec, 5.425 real sec [M::process] read 687674 sequences (100000296 bp) in 2.240 CPU sec, 2.253 real sec... [M::mem_process_seqs] Processed 688002 reads in 115.250 CPU sec, 3.627 real sec sorting block 53, #reads = 1082677 mem = 536883544 peak = 1061593088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 285598, 5, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 251, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (272.70, 124.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 291, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (311.61, 151.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 238, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1139) [M::mem_pestat] mean and std.dev: (272.00, 189.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1465) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687880 sequences (100000241 bp) in 2.819 CPU sec, 2.916 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 285557, 12, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 276, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (260.52, 101.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 289, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (309.84, 149.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (223, 2414, 2980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8494) [M::mem_pestat] mean and std.dev: (1931.08, 1675.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11251) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 281, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2970) [M::mem_pestat] mean and std.dev: (536.63, 600.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3888) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688260 reads in 170.901 CPU sec, 5.421 real sec sorting block 54, #reads = 1082742 mem = 536877716 peak = 1061593088 [M::process] read 687368 sequences (100000106 bp) in 2.418 CPU sec, 2.444 real sec... [M::mem_process_seqs] Processed 687674 reads in 116.530 CPU sec, 3.664 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 284704, 2, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 221, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1426) [M::mem_pestat] mean and std.dev: (323.52, 311.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 290, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (309.16, 148.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 206, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (235.96, 125.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 55, #reads = 1083289 mem = 536879038 peak = 1107308544 [M::process] read 687446 sequences (100000009 bp) in 2.840 CPU sec, 2.926 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285642, 5, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 275, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025) [M::mem_pestat] mean and std.dev: (270.23, 118.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 295, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (314.90, 153.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 293, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (260.26, 91.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687880 reads in 170.103 CPU sec, 5.409 real sec [M::mem_process_seqs] Processed 687368 reads in 115.783 CPU sec, 3.640 real sec [M::process] read 687214 sequences (100000296 bp) in 2.417 CPU sec, 2.453 real sec... sorting block 56, #reads = 1082755 mem = 536881344 peak = 1107308544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 285152, 10, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 237, 1751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4891) [M::mem_pestat] mean and std.dev: (634.30, 831.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 297, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (317.49, 155.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (341, 1022, 1286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3176) [M::mem_pestat] mean and std.dev: (1058.50, 995.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5039) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 251, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (281.29, 131.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1208) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 686872 sequences (100000201 bp) in 2.818 CPU sec, 2.915 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 285240, 2, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 299, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (292.63, 169.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1213) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 297, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (318.03, 156.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 270, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (258.68, 94.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687446 reads in 170.224 CPU sec, 5.400 real sec [M::mem_process_seqs] Processed 687214 reads in 117.944 CPU sec, 3.713 real sec [M::process] read 693914 sequences (100000047 bp) in 2.447 CPU sec, 2.470 real sec... sorting block 57, #reads = 1082774 mem = 536879955 peak = 1107308544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 284736, 3, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 297, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (256.09, 93.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (195, 300, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (321.17, 158.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 281, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811) [M::mem_pestat] mean and std.dev: (294.19, 175.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694748 sequences (100000295 bp) in 2.754 CPU sec, 2.824 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 287740, 6, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (122, 177, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668) [M::mem_pestat] mean and std.dev: (202.36, 108.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (295.03, 151.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1050) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 291, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (291.13, 125.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 686872 reads in 173.946 CPU sec, 5.520 real sec [M::process] read 692144 sequences (100000202 bp) in 2.194 CPU sec, 2.195 real sec... [M::mem_process_seqs] Processed 693914 reads in 124.116 CPU sec, 3.913 real sec sorting block 58, #reads = 1081763 mem = 536879110 peak = 1107308544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 287891, 11, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 262, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (249.48, 125.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (293.38, 151.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1326, 1580, 2558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5022) [M::mem_pestat] mean and std.dev: (1515.00, 997.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 290, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (320.58, 165.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 59, #reads = 1084175 mem = 536879535 peak = 1107308544 [M::process] read 691176 sequences (100000090 bp) in 2.825 CPU sec, 2.937 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 287508, 4, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 268, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (261.37, 95.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 277, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (303.89, 155.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 282, 474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (299.43, 152.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694748 reads in 179.530 CPU sec, 5.706 real sec [M::process] read 690436 sequences (100000089 bp) in 2.505 CPU sec, 2.534 real sec... [M::mem_process_seqs] Processed 692144 reads in 118.334 CPU sec, 3.718 real sec sorting block 60, #reads = 1086282 mem = 536878296 peak = 1107308544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 286328, 7, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 291, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199) [M::mem_pestat] mean and std.dev: (255.65, 123.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 280, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (307.26, 156.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1098) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 262, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (248.00, 89.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690214 sequences (100000172 bp) in 2.794 CPU sec, 2.888 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 286249, 5, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 347, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1144) [M::mem_pestat] mean and std.dev: (327.94, 157.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 284, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (310.68, 158.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 197, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (236.82, 111.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691176 reads in 173.600 CPU sec, 5.533 real sec [M::mem_process_seqs] Processed 690436 reads in 116.155 CPU sec, 3.654 real sec [M::process] read 689898 sequences (100000042 bp) in 2.589 CPU sec, 2.632 real sec... sorting block 61, #reads = 1086383 mem = 536878453 peak = 1107308544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 286299, 2, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 241, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (251.27, 103.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (184, 287, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (312.02, 157.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 325, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3599) [M::mem_pestat] mean and std.dev: (740.30, 815.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4733) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689560 sequences (100000226 bp) in 2.788 CPU sec, 2.891 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 286367, 7, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 279, 1255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3303) [M::mem_pestat] mean and std.dev: (609.04, 613.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 289, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (313.46, 158.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 280, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (255.06, 94.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690214 reads in 174.934 CPU sec, 5.554 real sec [M::mem_process_seqs] Processed 689898 reads in 119.482 CPU sec, 3.759 real sec [M::process] read 689392 sequences (100000008 bp) in 2.466 CPU sec, 2.491 real sec... sorting block 62, #reads = 1085096 mem = 536879672 peak = 1107308544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 286077, 10, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 229, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 596) [M::mem_pestat] mean and std.dev: (248.75, 124.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 290, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (314.19, 158.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (129, 899, 1964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5634) [M::mem_pestat] mean and std.dev: (1189.78, 1479.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7469) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 366, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1222) [M::mem_pestat] mean and std.dev: (353.56, 165.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688680 sequences (100000098 bp) in 2.852 CPU sec, 2.944 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 286311, 3, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 202, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (246.59, 146.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 291, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (315.49, 158.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 325, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (287.00, 117.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 689560 reads in 178.108 CPU sec, 5.672 real sec sorting block 63, #reads = 1084374 mem = 536878448 peak = 1107308544 [M::mem_process_seqs] Processed 689392 reads in 122.928 CPU sec, 3.863 real sec [M::process] read 688646 sequences (100000060 bp) in 2.595 CPU sec, 2.634 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 285539, 6, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 228, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (262.39, 163.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 293, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (316.07, 157.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 281, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (265.91, 125.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 64, #reads = 1083269 mem = 536880012 peak = 1107308544 [M::process] read 688596 sequences (100000293 bp) in 2.738 CPU sec, 2.815 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285444, 6, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 243, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (259.21, 124.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 294, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (316.67, 157.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 235, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (260.28, 117.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688680 reads in 179.280 CPU sec, 5.705 real sec [M::mem_process_seqs] Processed 688646 reads in 122.280 CPU sec, 3.843 real sec [M::process] read 688378 sequences (100000201 bp) in 2.522 CPU sec, 2.551 real sec... sorting block 65, #reads = 1082504 mem = 536880049 peak = 1107308544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 285418, 15, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 219, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (211.59, 58.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 293, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (316.39, 157.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1118) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (400, 520, 2298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6094) [M::mem_pestat] mean and std.dev: (875.36, 838.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7992) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 265, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (307.43, 170.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688172 sequences (100000241 bp) in 2.606 CPU sec, 2.702 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285385, 9, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 272, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (261.98, 140.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 293, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (315.43, 156.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 208, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1460) [M::mem_pestat] mean and std.dev: (268.35, 191.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1898) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688596 reads in 180.517 CPU sec, 5.732 real sec [M::process] read 687964 sequences (100000005 bp) in 2.260 CPU sec, 2.258 real sec... [M::mem_process_seqs] Processed 688378 reads in 123.953 CPU sec, 3.911 real sec sorting block 66, #reads = 1082134 mem = 536879621 peak = 1107308544 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 284969, 7, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 180, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (200.97, 90.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 293, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (315.30, 155.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 257, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (271.11, 128.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687894 sequences (100000183 bp) in 2.773 CPU sec, 2.842 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 284881, 5, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 251, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698) [M::mem_pestat] mean and std.dev: (259.43, 141.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 294, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (316.23, 156.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 178, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (231.53, 103.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688172 reads in 184.452 CPU sec, 5.848 real sec [M::process] read 688078 sequences (100000197 bp) in 2.360 CPU sec, 2.367 real sec... sorting block 67, #reads = 1080720 mem = 536880621 peak = 1107308544 [M::mem_process_seqs] Processed 687964 reads in 125.866 CPU sec, 3.968 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 285833, 9, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 263, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903) [M::mem_pestat] mean and std.dev: (275.87, 201.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 294, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (316.48, 156.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 263, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997) [M::mem_pestat] mean and std.dev: (268.47, 167.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687746 sequences (100000139 bp) in 2.524 CPU sec, 2.589 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 285357, 7, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 296, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (286.39, 146.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 293, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (314.92, 155.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 243, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (317.07, 201.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687430 sequences (100000079 bp) in 2.360 CPU sec, 2.361 real sec... [M::mem_process_seqs] Processed 687894 reads in 176.434 CPU sec, 5.596 real sec sorting block 68, #reads = 1080466 mem = 536880757 peak = 1107308544 [M::mem_process_seqs] Processed 688078 reads in 120.017 CPU sec, 3.805 real sec sorting block 69, #reads = 1081470 mem = 536876633 peak = 1157337088 [M::process] read 687520 sequences (100000115 bp) in 2.830 CPU sec, 2.914 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285839, 3, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 252, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (225.81, 74.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 296, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (317.76, 156.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 250, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (270.88, 144.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687430 reads in 100.585 CPU sec, 3.156 real sec [M::process] read 687086 sequences (100000198 bp) in 2.400 CPU sec, 2.410 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 285216, 3, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 224, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (236.38, 129.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 293, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (314.65, 154.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 317, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 562) [M::mem_pestat] mean and std.dev: (285.78, 105.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 285589, 6, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 222, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1284) [M::mem_pestat] mean and std.dev: (398.89, 349.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 295, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (317.83, 156.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 361, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (317.27, 139.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687746 reads in 276.687 CPU sec, 8.771 real sec sorting block 70, #reads = 1081676 mem = 536879178 peak = 1157337088 [M::process] read 687496 sequences (100000292 bp) in 2.711 CPU sec, 2.725 real sec... [M::mem_process_seqs] Processed 687520 reads in 144.147 CPU sec, 4.560 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 284898, 8, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 249, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (239.82, 90.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 297, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (320.21, 158.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 239, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (242.23, 118.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 71, #reads = 1081164 mem = 536878207 peak = 1157337088 [M::process] read 695084 sequences (100000092 bp) in 3.000 CPU sec, 3.106 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 285329, 5, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 264, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (287.89, 121.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (321.25, 160.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 246, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (229.95, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687086 reads in 180.621 CPU sec, 5.729 real sec [M::process] read 697662 sequences (100000113 bp) in 2.565 CPU sec, 2.596 real sec... [M::mem_process_seqs] Processed 687496 reads in 123.113 CPU sec, 3.867 real sec sorting block 72, #reads = 1080633 mem = 536879539 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 288062, 7, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 331, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (296.32, 132.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 262, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (290.51, 149.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 277, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (263.50, 98.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 696682 sequences (100000210 bp) in 3.005 CPU sec, 3.087 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 288900, 5, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 251, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (251.38, 111.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 252, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (280.40, 143.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 990) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 251, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (259.45, 117.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694784 sequences (100000030 bp) in 2.169 CPU sec, 2.179 real sec... [M::mem_process_seqs] Processed 695084 reads in 190.793 CPU sec, 6.038 real sec [M::mem_process_seqs] Processed 697662 reads in 129.126 CPU sec, 4.078 real sec sorting block 73, #reads = 1080929 mem = 536877062 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 288459, 8, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 271, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (293.70, 172.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 257, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (283.26, 143.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 318, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1515) [M::mem_pestat] mean and std.dev: (348.57, 289.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693724 sequences (100000018 bp) in 2.742 CPU sec, 2.822 real sec... [M::process] read 693118 sequences (100000126 bp) in 2.296 CPU sec, 2.298 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 288065, 3, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 238, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991) [M::mem_pestat] mean and std.dev: (264.32, 145.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 264, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (290.41, 146.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 269, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (321.85, 213.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696682 reads in 188.866 CPU sec, 5.973 real sec sorting block 74, #reads = 1084282 mem = 536882988 peak = 1157337088 [M::mem_process_seqs] Processed 694784 reads in 124.666 CPU sec, 3.948 real sec sorting block 75, #reads = 1083846 mem = 536878628 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 288128, 6, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 212, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (216.49, 108.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 269, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (293.73, 148.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 267, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002) [M::mem_pestat] mean and std.dev: (289.52, 144.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692212 sequences (100000217 bp) in 3.047 CPU sec, 3.126 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 288054, 10, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 206, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (255.76, 147.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (176, 272, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (297.64, 150.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1064) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (286, 816, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2794) [M::mem_pestat] mean and std.dev: (711.00, 473.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3630) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 229, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (282.64, 140.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 692246 sequences (100000178 bp) in 2.304 CPU sec, 2.305 real sec... [M::mem_process_seqs] Processed 693724 reads in 185.245 CPU sec, 5.851 real sec [M::mem_process_seqs] Processed 693118 reads in 123.339 CPU sec, 3.894 real sec sorting block 76, #reads = 1082381 mem = 536881703 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 287569, 7, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 216, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (305.14, 225.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 278, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (301.84, 151.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 255, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (308.54, 170.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691536 sequences (100000064 bp) in 2.928 CPU sec, 3.013 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 287673, 2, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 214, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (222.16, 81.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 277, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (300.97, 150.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 320, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (314.20, 145.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690756 sequences (100000117 bp) in 2.290 CPU sec, 2.296 real sec... [M::mem_process_seqs] Processed 692212 reads in 180.391 CPU sec, 5.690 real sec [M::mem_process_seqs] Processed 692246 reads in 121.530 CPU sec, 3.838 real sec sorting block 77, #reads = 1083116 mem = 536879484 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 287010, 8, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 259, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001) [M::mem_pestat] mean and std.dev: (290.83, 213.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (181, 281, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (304.33, 152.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 230, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (232.58, 98.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690176 sequences (100000051 bp) in 3.006 CPU sec, 3.061 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 287214, 2, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 232, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (216.81, 87.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 285, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (307.73, 153.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 301, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230) [M::mem_pestat] mean and std.dev: (305.19, 190.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1587) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689206 sequences (100000007 bp) in 2.200 CPU sec, 2.199 real sec... [M::mem_process_seqs] Processed 691536 reads in 174.356 CPU sec, 5.498 real sec sorting block 78, #reads = 1082246 mem = 536877636 peak = 1157337088 [M::mem_process_seqs] Processed 690756 reads in 115.543 CPU sec, 3.639 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 286236, 8, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 252, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (227.66, 72.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 287, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (310.90, 155.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1109) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 335, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015) [M::mem_pestat] mean and std.dev: (298.43, 177.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688990 sequences (100000067 bp) in 2.642 CPU sec, 2.688 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 285754, 5, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 250, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (245.41, 110.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 289, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (312.24, 155.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 320, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (285.45, 119.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688766 sequences (100000032 bp) in 1.880 CPU sec, 1.881 real sec... [M::mem_process_seqs] Processed 690176 reads in 170.820 CPU sec, 5.403 real sec sorting block 79, #reads = 1081434 mem = 536878216 peak = 1157337088 [M::mem_process_seqs] Processed 689206 reads in 122.880 CPU sec, 3.902 real sec sorting block 80, #reads = 1080388 mem = 536880179 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 286080, 7, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 361, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1725) [M::mem_pestat] mean and std.dev: (443.55, 321.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 290, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (312.85, 155.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 257, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (217.21, 66.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688426 sequences (100000233 bp) in 2.689 CPU sec, 2.742 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 285992, 12, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 367, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (309.92, 136.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1235) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 291, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878) [M::mem_pestat] mean and std.dev: (313.89, 156.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (737, 971, 2062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4712) [M::mem_pestat] mean and std.dev: (1228.67, 640.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6037) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 347, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1902) [M::mem_pestat] mean and std.dev: (371.11, 244.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2470) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688990 reads in 171.241 CPU sec, 5.408 real sec [M::process] read 688390 sequences (100000111 bp) in 2.230 CPU sec, 2.226 real sec... [M::mem_process_seqs] Processed 688766 reads in 115.228 CPU sec, 3.631 real sec sorting block 81, #reads = 1081320 mem = 536878305 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 285829, 10, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 289, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898) [M::mem_pestat] mean and std.dev: (289.04, 161.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 292, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (316.15, 157.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (102, 1162, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3630) [M::mem_pestat] mean and std.dev: (779.80, 522.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 252, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (291.13, 147.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1229) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688578 sequences (100000010 bp) in 2.719 CPU sec, 2.759 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 285898, 15, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 263, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (238.11, 110.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 293, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (315.86, 156.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (464, 579, 1806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4490) [M::mem_pestat] mean and std.dev: (866.08, 625.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5832) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 242, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (257.55, 109.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 687542 sequences (100000186 bp) in 2.250 CPU sec, 2.250 real sec... [M::mem_process_seqs] Processed 688426 reads in 168.816 CPU sec, 5.325 real sec [M::mem_process_seqs] Processed 688390 reads in 113.008 CPU sec, 3.560 real sec sorting block 82, #reads = 1081406 mem = 536879204 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 285756, 8, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 174, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (220.19, 138.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 290, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (313.06, 155.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 293, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (341.33, 192.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687062 sequences (100000221 bp) in 2.895 CPU sec, 2.953 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 285198, 14, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 245, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (271.29, 167.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 296, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (318.99, 158.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1120) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (240, 816, 1131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2913) [M::mem_pestat] mean and std.dev: (722.85, 597.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 355, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (321.13, 132.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688578 reads in 169.233 CPU sec, 5.336 real sec [M::process] read 687310 sequences (100000252 bp) in 2.270 CPU sec, 2.264 real sec... [M::mem_process_seqs] Processed 687542 reads in 114.563 CPU sec, 3.606 real sec sorting block 83, #reads = 1080622 mem = 536878124 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 285369, 14, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 228, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (256.91, 147.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (319.62, 157.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (309, 2277, 7312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21318) [M::mem_pestat] mean and std.dev: (2886.93, 2931.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 28321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 310, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (304.31, 129.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 84, #reads = 1081058 mem = 536879677 peak = 1157337088 [M::process] read 688346 sequences (100000132 bp) in 2.466 CPU sec, 2.522 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 285121, 9, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 188, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (222.68, 121.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 298, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (320.77, 159.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 291, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244) [M::mem_pestat] mean and std.dev: (350.57, 186.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1588) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687062 reads in 166.737 CPU sec, 5.300 real sec [M::process] read 695136 sequences (100000282 bp) in 2.403 CPU sec, 2.419 real sec... [M::mem_process_seqs] Processed 687310 reads in 116.161 CPU sec, 3.647 real sec sorting block 85, #reads = 1081280 mem = 536881588 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 285313, 6, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 312, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1275) [M::mem_pestat] mean and std.dev: (266.27, 173.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 292, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888) [M::mem_pestat] mean and std.dev: (317.20, 160.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 251, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (276.60, 156.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1254) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 694748 sequences (100000161 bp) in 2.647 CPU sec, 2.691 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 288106, 8, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 222, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (305.70, 207.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (290.93, 149.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 237, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (262.43, 155.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693464 sequences (100000144 bp) in 2.220 CPU sec, 2.223 real sec... [M::mem_process_seqs] Processed 688346 reads in 182.126 CPU sec, 5.758 real sec [M::mem_process_seqs] Processed 695136 reads in 124.195 CPU sec, 3.926 real sec sorting block 86, #reads = 1077887 mem = 536879019 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 287820, 7, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 249, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025) [M::mem_pestat] mean and std.dev: (316.86, 204.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1302) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (173, 265, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (291.47, 148.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 265, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (284.51, 117.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692450 sequences (100000136 bp) in 2.760 CPU sec, 2.815 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 288091, 3, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 281, 444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018) [M::mem_pestat] mean and std.dev: (259.22, 119.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (295.49, 149.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 270, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (280.98, 140.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 694748 reads in 168.955 CPU sec, 5.333 real sec [M::process] read 691918 sequences (100000155 bp) in 2.220 CPU sec, 2.224 real sec... [M::mem_process_seqs] Processed 693464 reads in 111.793 CPU sec, 3.513 real sec sorting block 87, #reads = 1084484 mem = 536880300 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 286905, 6, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 288, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1420) [M::mem_pestat] mean and std.dev: (319.18, 187.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 275, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (298.78, 149.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 311, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (265.42, 117.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1310) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691284 sequences (100000204 bp) in 2.935 CPU sec, 2.992 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 286755, 6, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 237, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (266.22, 141.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 279, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (301.47, 150.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1068) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 299, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (242.33, 87.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 692450 reads in 167.678 CPU sec, 5.293 real sec [M::process] read 690828 sequences (100000056 bp) in 2.208 CPU sec, 2.215 real sec... sorting block 88, #reads = 1088073 mem = 536878936 peak = 1157337088 [M::mem_process_seqs] Processed 691918 reads in 111.971 CPU sec, 3.521 real sec sorting block 89, #reads = 1085357 mem = 536877440 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 287343, 6, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 244, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (255.94, 145.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 281, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (303.07, 150.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 199, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (268.78, 189.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1216) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 690874 sequences (100000162 bp) in 2.275 CPU sec, 2.332 real sec... [M::process] read 690950 sequences (100000248 bp) in 1.795 CPU sec, 1.797 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 285797, 8, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 278, 737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1815) [M::mem_pestat] mean and std.dev: (394.12, 252.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 284, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (305.82, 151.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 264, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1485) [M::mem_pestat] mean and std.dev: (376.32, 282.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1921) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 691284 reads in 169.949 CPU sec, 5.398 real sec [M::mem_process_seqs] Processed 690828 reads in 120.475 CPU sec, 3.822 real sec sorting block 90, #reads = 1085956 mem = 536879264 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 286562, 12, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 298, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (307.74, 162.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 285, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (306.52, 152.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 777, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1811) [M::mem_pestat] mean and std.dev: (603.33, 393.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2328) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 175, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (259.12, 192.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 690462 sequences (100000076 bp) in 2.464 CPU sec, 2.530 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 286341, 5, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 256, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981) [M::mem_pestat] mean and std.dev: (310.47, 160.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1245) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 283, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (305.35, 151.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1086) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 193, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (202.30, 81.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689768 sequences (100000228 bp) in 2.210 CPU sec, 2.205 real sec... [M::mem_process_seqs] Processed 690874 reads in 168.543 CPU sec, 5.324 real sec [M::mem_process_seqs] Processed 690950 reads in 112.112 CPU sec, 3.539 real sec sorting block 91, #reads = 1083591 mem = 536878797 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 286685, 6, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 244, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (227.04, 117.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (185, 286, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (306.92, 150.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1085) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 273, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (273.59, 131.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 691092 sequences (100000238 bp) in 2.543 CPU sec, 2.595 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 285846, 8, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 256, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (242.68, 80.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 287, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (307.72, 151.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 342, 828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2080) [M::mem_pestat] mean and std.dev: (562.82, 517.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2706) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 690462 reads in 165.187 CPU sec, 5.220 real sec [M::process] read 689944 sequences (100000096 bp) in 2.136 CPU sec, 2.139 real sec... sorting block 92, #reads = 1084578 mem = 536877483 peak = 1157337088 [M::mem_process_seqs] Processed 689768 reads in 110.624 CPU sec, 3.480 real sec sorting block 93, #reads = 1083648 mem = 536880530 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 286347, 9, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 169, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (202.44, 106.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (182, 280, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (302.65, 149.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 281, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2215) [M::mem_pestat] mean and std.dev: (446.36, 386.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689250 sequences (100000001 bp) in 2.619 CPU sec, 2.675 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 285747, 12, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 439, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1917) [M::mem_pestat] mean and std.dev: (594.23, 552.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 288, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (309.36, 153.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 204, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (135.20, 91.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 265, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (274.30, 113.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 689264 sequences (100000036 bp) in 2.190 CPU sec, 2.186 real sec... [M::mem_process_seqs] Processed 691092 reads in 168.005 CPU sec, 5.302 real sec [M::mem_process_seqs] Processed 689944 reads in 114.173 CPU sec, 3.600 real sec sorting block 94, #reads = 1083503 mem = 536880764 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285779, 7, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 272, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (241.61, 107.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 290, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (311.76, 153.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 234, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (250.71, 130.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688950 sequences (100000109 bp) in 2.716 CPU sec, 2.769 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 286484, 3, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 278, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019) [M::mem_pestat] mean and std.dev: (280.80, 149.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 292, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (313.67, 155.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 200, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (192.39, 63.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689260 sequences (100000177 bp) in 2.150 CPU sec, 2.156 real sec... [M::mem_process_seqs] Processed 689250 reads in 175.221 CPU sec, 5.543 real sec [M::mem_process_seqs] Processed 689264 reads in 118.945 CPU sec, 3.758 real sec sorting block 95, #reads = 1081353 mem = 536876914 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 286304, 7, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 156, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (196.25, 90.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 289, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (312.70, 155.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1103) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 256, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (269.47, 99.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688420 sequences (100000175 bp) in 2.745 CPU sec, 2.811 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 285763, 10, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 266, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (291.59, 171.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (187, 287, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (309.29, 152.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (106, 328, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1645) [M::mem_pestat] mean and std.dev: (320.33, 234.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2158) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 246, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (248.67, 104.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688706 sequences (100000148 bp) in 2.260 CPU sec, 2.266 real sec... [M::mem_process_seqs] Processed 688950 reads in 183.639 CPU sec, 5.814 real sec [M::mem_process_seqs] Processed 689260 reads in 125.802 CPU sec, 3.972 real sec sorting block 96, #reads = 1081294 mem = 536877912 peak = 1157337088 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 285136, 5, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 228, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (207.23, 71.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 291, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (312.95, 154.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 216, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (238.81, 121.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688812 sequences (100000216 bp) in 2.617 CPU sec, 2.670 real sec... [M::process] read 688084 sequences (100000260 bp) in 1.884 CPU sec, 1.889 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 286013, 14, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (136, 187, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (221.31, 119.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 291, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (313.72, 155.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (627, 912, 1298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2640) [M::mem_pestat] mean and std.dev: (739.27, 369.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3311) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 270, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152) [M::mem_pestat] mean and std.dev: (338.43, 206.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1470) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688420 reads in 179.837 CPU sec, 5.699 real sec sorting block 97, #reads = 1080444 mem = 536877665 peak = 1157337088 [M::mem_process_seqs] Processed 688706 reads in 120.174 CPU sec, 3.809 real sec sorting block 98, #reads = 1080440 mem = 536879229 peak = 1168138240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 285675, 8, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 227, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (213.26, 119.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (189, 291, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (314.30, 156.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 278, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (303.94, 137.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688984 sequences (100000204 bp) in 2.807 CPU sec, 2.894 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 285439, 10, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 197, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (205.97, 75.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 292, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874) [M::mem_pestat] mean and std.dev: (314.90, 156.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1102) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (246, 346, 2221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6171) [M::mem_pestat] mean and std.dev: (942.60, 901.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8146) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 302, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (293.76, 166.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699388 sequences (100000026 bp) in 2.296 CPU sec, 2.302 real sec... [M::mem_process_seqs] Processed 688812 reads in 191.755 CPU sec, 6.080 real sec [M::mem_process_seqs] Processed 688084 reads in 130.350 CPU sec, 4.121 real sec sorting block 99, #reads = 1078016 mem = 536879561 peak = 1168138240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 285625, 5, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 271, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (257.43, 109.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 290, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (313.39, 156.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 315, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1193) [M::mem_pestat] mean and std.dev: (323.48, 165.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1519) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 697986 sequences (100000029 bp) in 2.786 CPU sec, 2.845 real sec... [M::process] read 696554 sequences (100000071 bp) in 2.380 CPU sec, 2.381 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 289543, 8, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 236, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770) [M::mem_pestat] mean and std.dev: (242.23, 108.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (273.36, 139.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 207, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (225.33, 115.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 688984 reads in 206.029 CPU sec, 6.526 real sec [M::mem_process_seqs] Processed 699388 reads in 136.591 CPU sec, 4.328 real sec sorting block 100, #reads = 1074398 mem = 536879548 peak = 1168138240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 289209, 9, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 213, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1246) [M::mem_pestat] mean and std.dev: (257.77, 149.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 252, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (278.95, 141.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 270, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (299.56, 173.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 695332 sequences (100000278 bp) in 2.831 CPU sec, 2.884 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 288658, 7, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 243, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1109) [M::mem_pestat] mean and std.dev: (241.75, 125.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (169, 259, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (283.64, 142.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 286, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (323.18, 158.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693954 sequences (100000006 bp) in 2.300 CPU sec, 2.303 real sec... [M::mem_process_seqs] Processed 697986 reads in 190.167 CPU sec, 6.010 real sec sorting block 101, #reads = 1081024 mem = 536879711 peak = 1168138240 [M::mem_process_seqs] Processed 696554 reads in 126.857 CPU sec, 4.015 real sec sorting block 102, #reads = 1083967 mem = 536880300 peak = 1168138240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 288319, 16, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 177, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (201.59, 77.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (172, 264, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (287.74, 143.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (79, 1565, 2508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7366) [M::mem_pestat] mean and std.dev: (1074.07, 921.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9795) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 199, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (203.54, 60.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 693256 sequences (100000244 bp) in 2.460 CPU sec, 2.554 real sec... [M::process] read 692394 sequences (100000301 bp) in 1.910 CPU sec, 1.918 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 288104, 7, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (126, 188, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (210.32, 147.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1374) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (175, 271, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (293.78, 146.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 307, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806) [M::mem_pestat] mean and std.dev: (264.38, 127.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 695332 reads in 186.555 CPU sec, 5.914 real sec [M::mem_process_seqs] Processed 693954 reads in 126.534 CPU sec, 4.030 real sec sorting block 103, #reads = 1081651 mem = 536879856 peak = 1168138240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 287747, 3, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 292, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078) [M::mem_pestat] mean and std.dev: (326.31, 252.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 274, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 834) [M::mem_pestat] mean and std.dev: (296.17, 146.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 254, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (277.59, 120.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 692838 sequences (100000021 bp) in 2.558 CPU sec, 2.603 real sec... [M::process] read 692168 sequences (100000130 bp) in 2.177 CPU sec, 2.185 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 287318, 4, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (125, 231, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (183.96, 70.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (179, 278, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (300.53, 149.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 287, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (338.94, 188.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 693256 reads in 184.619 CPU sec, 5.844 real sec [M::mem_process_seqs] Processed 692394 reads in 121.883 CPU sec, 3.870 real sec sorting block 104, #reads = 1081692 mem = 536879334 peak = 1168138240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (56, 287241, 5, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 251, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (277.86, 130.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (178, 276, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (299.34, 149.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 250, 1014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2650) [M::mem_pestat] mean and std.dev: (594.14, 713.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 693008 sequences (100000038 bp) in 2.840 CPU sec, 2.876 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 287219, 10, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (139, 203, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (210.87, 103.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 280, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 861) [M::mem_pestat] mean and std.dev: (303.42, 152.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 527, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1535) [M::mem_pestat] mean and std.dev: (364.22, 273.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2039) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 295, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (286.27, 110.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 691020 sequences (100000107 bp) in 2.380 CPU sec, 2.385 real sec... [M::mem_process_seqs] Processed 692838 reads in 189.516 CPU sec, 5.987 real sec [M::mem_process_seqs] Processed 692168 reads in 127.056 CPU sec, 4.022 real sec sorting block 105, #reads = 1081021 mem = 536878016 peak = 1168138240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 287544, 6, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 188, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (228.65, 166.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (297.70, 149.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 335, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1377) [M::mem_pestat] mean and std.dev: (389.67, 222.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1763) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689518 sequences (100000076 bp) in 2.657 CPU sec, 2.747 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 286533, 9, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 199, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (221.14, 90.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (183, 280, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (302.16, 148.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 270, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (303.00, 143.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 689282 sequences (100000211 bp) in 2.018 CPU sec, 2.028 real sec... [M::mem_process_seqs] Processed 693008 reads in 190.834 CPU sec, 6.073 real sec sorting block 106, #reads = 1080837 mem = 536879255 peak = 1168138240 [M::mem_process_seqs] Processed 691020 reads in 129.397 CPU sec, 4.104 real sec sorting block 107, #reads = 1081325 mem = 536883196 peak = 1168138240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 285927, 10, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 322, 1265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3415) [M::mem_pestat] mean and std.dev: (631.79, 722.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 287, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (308.29, 151.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1082) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (512, 745, 1236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2684) [M::mem_pestat] mean and std.dev: (940.90, 757.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3972) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 272, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (307.19, 119.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688570 sequences (100000161 bp) in 2.732 CPU sec, 2.831 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 285739, 12, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 263, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1405) [M::mem_pestat] mean and std.dev: (291.37, 204.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1815) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 289, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (309.09, 151.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (339, 976, 1362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3408) [M::mem_pestat] mean and std.dev: (883.58, 650.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4431) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 275, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (282.27, 102.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 688734 sequences (100000201 bp) in 2.363 CPU sec, 2.365 real sec... [M::mem_process_seqs] Processed 689518 reads in 190.788 CPU sec, 6.043 real sec [M::mem_process_seqs] Processed 689282 reads in 126.341 CPU sec, 3.995 real sec sorting block 108, #reads = 1080958 mem = 536885973 peak = 1168138240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 285370, 8, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 228, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (244.03, 111.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1247) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 288, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (309.08, 150.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 320, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (305.29, 141.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1149) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 688220 sequences (100000221 bp) in 2.862 CPU sec, 2.926 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 286128, 9, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 241, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036) [M::mem_pestat] mean and std.dev: (295.16, 194.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (188, 286, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (306.57, 149.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 249, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 743) [M::mem_pestat] mean and std.dev: (273.11, 143.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687912 sequences (100000050 bp) in 2.353 CPU sec, 2.356 real sec... [M::mem_process_seqs] Processed 688570 reads in 187.697 CPU sec, 5.935 real sec [M::mem_process_seqs] Processed 688734 reads in 125.555 CPU sec, 3.970 real sec sorting block 109, #reads = 1079740 mem = 536878492 peak = 1168138240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 285848, 6, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 179, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (172.32, 71.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 986) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 288, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 841) [M::mem_pestat] mean and std.dev: (308.55, 149.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 240, 754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870) [M::mem_pestat] mean and std.dev: (380.17, 349.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2428) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687794 sequences (100000189 bp) in 2.629 CPU sec, 2.684 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 285726, 8, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 226, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (255.06, 132.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (190, 289, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (309.76, 150.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1066) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 257, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (301.17, 176.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687350 sequences (100000174 bp) in 2.077 CPU sec, 2.071 real sec... [M::mem_process_seqs] Processed 688220 reads in 175.383 CPU sec, 5.561 real sec sorting block 110, #reads = 1081727 mem = 536882864 peak = 1168138240 [M::mem_process_seqs] Processed 687912 reads in 115.505 CPU sec, 3.648 real sec sorting block 111, #reads = 1081985 mem = 536884621 peak = 1168138240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 285477, 4, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 308, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1453) [M::mem_pestat] mean and std.dev: (409.31, 246.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 293, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (314.81, 154.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 287, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991) [M::mem_pestat] mean and std.dev: (321.07, 150.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1249) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::process] read 687286 sequences (100000026 bp) in 2.453 CPU sec, 2.487 real sec... [M::process] read 648478 sequences (94353922 bp) in 1.580 CPU sec, 1.579 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 285461, 8, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 222, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (273.39, 144.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1116) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 297, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (318.14, 156.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1105) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 240, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (261.56, 131.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687794 reads in 165.945 CPU sec, 5.260 real sec [M::mem_process_seqs] Processed 687350 reads in 111.824 CPU sec, 3.550 real sec sorting block 112, #reads = 1082020 mem = 536880361 peak = 1168138240 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 285554, 10, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 219, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (251.32, 99.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (194, 298, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884) [M::mem_pestat] mean and std.dev: (319.95, 158.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1114) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (587, 801, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1592) [M::mem_pestat] mean and std.dev: (697.78, 390.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 281, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (252.33, 117.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 268965, 9, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 294, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (273.50, 115.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (193, 297, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883) [M::mem_pestat] mean and std.dev: (319.18, 158.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1113) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 238, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (252.90, 85.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 687286 reads in 154.690 CPU sec, 5.003 real sec [M::mem_process_seqs] Processed 648478 reads in 104.986 CPU sec, 3.397 real sec sorting block 113, #reads = 1081430 mem = 536883268 peak = 1168138240 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: INTEL(R) XEON(R) PLATINUM 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 123355574 overall: 69806411776 mem 18728.702 user 467.065 sys 625.108 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_5KC-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:1136_5KC-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_5KC-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_5KC-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_5KC-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 625.145 sec; CPU: 19195.770 sec sorting block 114, #reads = 579939 mem = 288467360 peak = 1168138240 uniq header count 1 merging 115 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: INTEL(R) XEON(R) PLATINUM 8559C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 6868368365 output reads: 124060689 bam_mem_sort: 115 calls 65.192 user 1.262 sys 66.621 real bam_write: 115 calls 54.849 user 3.054 sys 63.630 real execute: 1 calls 38.848 user 2.035 sys 680.103 real merge_files: 1 calls 38.819 user 2.005 sys 54.453 real parse_chunk: 1833 calls 104.782 user 24.876 sys 132.988 real read_chunk: 4947 calls 55.262 user 32.035 sys 577.767 real sort_block: 1 calls 37.350 user 31.393 sys 553.054 real write_chunk: 3112 calls 38.350 user 1.708 sys 53.290 real overall: 3142029312 mem 2100.327 user 92.407 sys 680.126 real ls: cannot access '*.cram': No such file or directory