.command.log - Workdir - DAQ:SENTIEON_BWAMEM (1029_N5D-T1-TDNA-1_A23MHWWLT4_1;L006;0002)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2f/be42e4b7c8204c46e83da478a44ca0/.command.log
Size: 137.1 KB
Task: DAQ:SENTIEON_BWAMEM (1029_N5D-T1-TDNA-1_A23MHWWLT4_1;L006;0002)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1c/02021c3813b24f42b8edd55722f6e9/1029_N5D-T1-TDNA-1_A23MHWWLT4_1/0002.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2f/be42e4b7c8204c46e83da478a44ca0/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1c/02021c3813b24f42b8edd55722f6e9/1029_N5D-T1-TDNA-1_A23MHWWLT4_1/0002.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2f/be42e4b7c8204c46e83da478a44ca0/.command.run
==> STAGING COMPLETE (7 inputs)

This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1029_N5D-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1243.665 CPU sec, 1000.565 real sec
[M::process] read 686234 sequences (100000205 bp) in 0.977 CPU sec, 0.982 real sec...
[M::process] read 684528 sequences (100000221 bp) in 1.186 CPU sec, 1.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 279128, 8, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 203, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (227.94, 117.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 760)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 317, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (337.21, 167.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 289, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.90, 91.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683502 sequences (100000097 bp) in 1.200 CPU sec, 1.223 real sec...
[M::process] read 682742 sequences (100000078 bp) in 1.159 CPU sec, 1.160 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279188, 5, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 277, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725)
[M::mem_pestat] mean and std.dev: (262.50, 117.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (345.93, 168.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 190, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463)
[M::mem_pestat] mean and std.dev: (194.26, 71.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 564)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 686234 reads in 147.672 CPU sec, 4.691 real sec
[M::mem_process_seqs] Processed 684528 reads in 91.001 CPU sec, 2.912 real sec
sorting block 0, #reads = 1070819 mem = 536883301 peak = 603074560
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278752, 5, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 321, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845)
[M::mem_pestat] mean and std.dev: (284.37, 123.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 336, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (352.18, 170.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 255, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (312.04, 153.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1330)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682150 sequences (100000235 bp) in 1.751 CPU sec, 1.926 real sec...
[M::process] read 681734 sequences (100000050 bp) in 1.170 CPU sec, 1.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278941, 2, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 198, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850)
[M::mem_pestat] mean and std.dev: (256.19, 150.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 339, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (356.04, 171.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 286, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (358.78, 181.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683502 reads in 135.081 CPU sec, 4.296 real sec
[M::mem_process_seqs] Processed 682742 reads in 89.725 CPU sec, 2.865 real sec
sorting block 1, #reads = 1070494 mem = 536881300 peak = 733622272
[M::process] read 681598 sequences (100000244 bp) in 1.486 CPU sec, 1.538 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278482, 8, 30)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (298, 360, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 718)
[M::mem_pestat] mean and std.dev: (318.18, 105.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 858)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 344, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (360.47, 174.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 351, 627)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1411)
[M::mem_pestat] mean and std.dev: (388.81, 259.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1803)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681594 sequences (100000160 bp) in 1.213 CPU sec, 1.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278340, 15, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 246, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562)
[M::mem_pestat] mean and std.dev: (248.81, 99.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 680)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (360.13, 172.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (812, 812, 2105)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4691)
[M::mem_pestat] mean and std.dev: (997.62, 675.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5984)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 209, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (265.63, 135.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278142, 1, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 237, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (255.90, 123.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 860)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 346, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (361.79, 173.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 252, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (316.30, 173.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682150 reads in 200.672 CPU sec, 6.454 real sec
[M::mem_process_seqs] Processed 681598 reads in 154.611 CPU sec, 5.007 real sec
sorting block 2, #reads = 1070594 mem = 536878981 peak = 888598528
[M::mem_process_seqs] Processed 681734 reads in 170.229 CPU sec, 5.533 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 277965, 0, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 162, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458)
[M::mem_pestat] mean and std.dev: (185.42, 82.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 566)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 339, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (355.22, 168.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (287, 371, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851)
[M::mem_pestat] mean and std.dev: (339.49, 108.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1039)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 3, #reads = 1070182 mem = 536879929 peak = 896638976
[M::process] read 681944 sequences (100000275 bp) in 2.449 CPU sec, 2.758 real sec...
[M::mem_process_seqs] Processed 681594 reads in 81.847 CPU sec, 2.709 real sec
sorting block 4, #reads = 1070130 mem = 536880255 peak = 903966720
[M::process] read 682240 sequences (100000157 bp) in 2.013 CPU sec, 2.239 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277791, 6, 29)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 323, 585)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1365)
[M::mem_pestat] mean and std.dev: (417.63, 358.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1851)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (350.46, 166.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 247, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829)
[M::mem_pestat] mean and std.dev: (253.32, 134.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1043)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681448 sequences (100000014 bp) in 1.333 CPU sec, 1.373 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 278548, 8, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 271, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589)
[M::mem_pestat] mean and std.dev: (263.12, 111.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 727)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (350.73, 166.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 297, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127)
[M::mem_pestat] mean and std.dev: (316.71, 170.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1439)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681944 reads in 145.710 CPU sec, 4.717 real sec
[M::process] read 681562 sequences (100000236 bp) in 1.294 CPU sec, 1.292 real sec...
[M::mem_process_seqs] Processed 682240 reads in 96.049 CPU sec, 3.043 real sec
sorting block 5, #reads = 1069612 mem = 536878704 peak = 903966720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278479, 3, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 191, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (254.93, 152.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 340, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (354.79, 167.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 334, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072)
[M::mem_pestat] mean and std.dev: (328.09, 166.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1362)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685110 sequences (100000036 bp) in 2.247 CPU sec, 2.462 real sec...
[M::process] read 684226 sequences (100000225 bp) in 1.290 CPU sec, 1.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278179, 7, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 295, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086)
[M::mem_pestat] mean and std.dev: (295.12, 178.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1399)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 341, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (357.37, 170.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 397, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1154)
[M::mem_pestat] mean and std.dev: (343.83, 221.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1485)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681448 reads in 143.676 CPU sec, 4.572 real sec
[M::mem_process_seqs] Processed 681562 reads in 95.444 CPU sec, 3.046 real sec
sorting block 6, #reads = 1067685 mem = 536881908 peak = 903966720
[M::process] read 683442 sequences (100000239 bp) in 1.732 CPU sec, 1.777 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279006, 10, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (253, 377, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (324.45, 135.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 325, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (344.54, 170.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (805, 1092, 1374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2512)
[M::mem_pestat] mean and std.dev: (1028.11, 528.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3143)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 289, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (279.39, 109.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682670 sequences (100000022 bp) in 1.290 CPU sec, 1.300 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278737, 4, 18)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 226, 348)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (230.26, 113.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 338, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (353.95, 171.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 440, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (372.35, 150.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278713, 11, 50)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (139, 251, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (257.33, 147.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 333, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (349.68, 170.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (114, 303, 616)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1620)
[M::mem_pestat] mean and std.dev: (323.40, 330.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2122)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 300, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (331.04, 151.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685110 reads in 200.624 CPU sec, 6.424 real sec
sorting block 7, #reads = 1068640 mem = 536878926 peak = 954191872
[M::mem_process_seqs] Processed 683442 reads in 151.492 CPU sec, 4.872 real sec
[M::mem_process_seqs] Processed 684226 reads in 166.850 CPU sec, 5.392 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278777, 1, 44)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 265, 548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294)
[M::mem_pestat] mean and std.dev: (288.21, 165.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1667)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 342, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (357.57, 172.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 315, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 840)
[M::mem_pestat] mean and std.dev: (335.77, 118.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 8, #reads = 1071716 mem = 536879411 peak = 954191872
[M::mem_process_seqs] Processed 682670 reads in 79.464 CPU sec, 2.647 real sec
[M::process] read 682064 sequences (100000123 bp) in 2.576 CPU sec, 2.859 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278345, 3, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 343, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864)
[M::mem_pestat] mean and std.dev: (303.06, 115.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 346, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (360.34, 172.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 303, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (292.35, 87.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681808 sequences (100000122 bp) in 2.047 CPU sec, 2.248 real sec...
sorting block 9, #reads = 1071850 mem = 536880411 peak = 954191872
[M::mem_process_seqs] Processed 682064 reads in 80.851 CPU sec, 2.658 real sec
[M::process] read 681658 sequences (100000007 bp) in 1.882 CPU sec, 2.025 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278445, 2, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 276, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (230.80, 104.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 956)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 347, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (361.58, 172.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 309, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (284.24, 100.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681672 sequences (100000107 bp) in 1.389 CPU sec, 1.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 278250, 6, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 281, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 874)
[M::mem_pestat] mean and std.dev: (295.56, 130.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (359.04, 170.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 209, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613)
[M::mem_pestat] mean and std.dev: (224.03, 96.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681590 sequences (100000105 bp) in 1.240 CPU sec, 1.239 real sec...
[M::mem_process_seqs] Processed 681808 reads in 144.059 CPU sec, 4.562 real sec
sorting block 10, #reads = 1071416 mem = 536879395 peak = 954191872
[M::mem_process_seqs] Processed 681658 reads in 95.144 CPU sec, 3.020 real sec
sorting block 11, #reads = 1069843 mem = 536879197 peak = 980422656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 278050, 5, 55)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 284, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (252.35, 112.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 781)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 340, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (355.13, 168.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 307, 587)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1299)
[M::mem_pestat] mean and std.dev: (364.83, 219.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1655)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681482 sequences (100000167 bp) in 2.206 CPU sec, 2.289 real sec...
[M::process] read 681648 sequences (100000283 bp) in 1.450 CPU sec, 1.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278107, 5, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 284, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (253.14, 111.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (353.59, 167.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 200, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733)
[M::mem_pestat] mean and std.dev: (207.72, 85.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 926)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681672 reads in 147.966 CPU sec, 4.709 real sec
[M::mem_process_seqs] Processed 681590 reads in 97.064 CPU sec, 3.094 real sec
sorting block 12, #reads = 1068628 mem = 536878464 peak = 980422656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278435, 9, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 276, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (258.60, 117.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1046)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (354.59, 168.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 322, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (346.78, 161.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681510 sequences (100000037 bp) in 2.389 CPU sec, 2.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 278695, 5, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 246, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (229.62, 82.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 340, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (356.10, 169.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 272, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (318.50, 173.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686284 sequences (100000262 bp) in 1.340 CPU sec, 1.338 real sec...
[M::mem_process_seqs] Processed 681482 reads in 143.513 CPU sec, 4.559 real sec
[M::mem_process_seqs] Processed 681648 reads in 92.755 CPU sec, 2.949 real sec
sorting block 13, #reads = 1069197 mem = 536883116 peak = 980422656
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 278064, 4, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (143, 244, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (283.07, 191.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 345, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (360.52, 172.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 273, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (281.89, 132.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685540 sequences (100000151 bp) in 2.266 CPU sec, 2.517 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 279308, 6, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 324, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (273.68, 100.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 889)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 318, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950)
[M::mem_pestat] mean and std.dev: (338.64, 168.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 247, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066)
[M::mem_pestat] mean and std.dev: (278.47, 146.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1365)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681510 reads in 141.230 CPU sec, 4.494 real sec
[M::process] read 684486 sequences (100000117 bp) in 1.435 CPU sec, 1.440 real sec...
sorting block 14, #reads = 1068314 mem = 536878600 peak = 980422656
[M::mem_process_seqs] Processed 686284 reads in 94.404 CPU sec, 2.994 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279428, 5, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (213, 260, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (308.77, 181.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 323, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (342.46, 169.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 393, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (309.30, 121.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 15, #reads = 1069713 mem = 536879780 peak = 1011003392
[M::process] read 683872 sequences (100000150 bp) in 2.013 CPU sec, 2.092 real sec...
[M::process] read 682820 sequences (100000053 bp) in 1.360 CPU sec, 1.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 279600, 4, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 224, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (234.42, 88.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 331, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (348.47, 170.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 263, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (280.66, 120.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 685540 reads in 138.630 CPU sec, 4.416 real sec
[M::mem_process_seqs] Processed 684486 reads in 91.704 CPU sec, 2.923 real sec
sorting block 16, #reads = 1071945 mem = 536877397 peak = 1011003392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279042, 12, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 301, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (295.09, 131.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 336, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (352.45, 171.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1245)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1432, 1543, 1884)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (528, 2788)
[M::mem_pestat] mean and std.dev: (1555.60, 287.39)
[M::mem_pestat] low and high boundaries for proper pairs: (76, 3240)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 405, 708)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1684)
[M::mem_pestat] mean and std.dev: (469.47, 324.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2172)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682632 sequences (100000094 bp) in 2.291 CPU sec, 2.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (37, 278735, 4, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 303, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (293.60, 149.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 342, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (357.71, 173.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 316, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (321.76, 135.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 929)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682700 sequences (100000038 bp) in 1.410 CPU sec, 1.413 real sec...
[M::mem_process_seqs] Processed 683872 reads in 139.518 CPU sec, 4.431 real sec
[M::mem_process_seqs] Processed 682820 reads in 93.206 CPU sec, 2.961 real sec
sorting block 17, #reads = 1070368 mem = 536877888 peak = 1011003392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277947, 1, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 286, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1121)
[M::mem_pestat] mean and std.dev: (268.03, 141.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1436)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 344, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (359.49, 173.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (280, 315, 943)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2269)
[M::mem_pestat] mean and std.dev: (426.82, 319.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2932)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682548 sequences (100000101 bp) in 2.082 CPU sec, 2.153 real sec...
[M::process] read 681402 sequences (100000257 bp) in 1.380 CPU sec, 1.386 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278723, 8, 35)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 254, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (281.29, 217.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 336, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (353.74, 170.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 257, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (269.36, 123.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682632 reads in 140.754 CPU sec, 4.476 real sec
[M::mem_process_seqs] Processed 682700 reads in 92.947 CPU sec, 2.965 real sec
sorting block 18, #reads = 1070455 mem = 536878452 peak = 1011003392
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 278305, 11, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 272, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1196)
[M::mem_pestat] mean and std.dev: (345.88, 259.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1533)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 337, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974)
[M::mem_pestat] mean and std.dev: (354.41, 170.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (430, 1353, 5999)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17137)
[M::mem_pestat] mean and std.dev: (2683.64, 2657.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 22706)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 235, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (278.33, 124.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681732 sequences (100000292 bp) in 2.208 CPU sec, 2.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 277850, 9, 53)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 248, 580)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1356)
[M::mem_pestat] mean and std.dev: (442.70, 348.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1837)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 345, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (359.95, 171.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 361, 633)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1487)
[M::mem_pestat] mean and std.dev: (371.18, 233.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1914)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681720 sequences (100000013 bp) in 1.420 CPU sec, 1.418 real sec...
[M::mem_process_seqs] Processed 682548 reads in 142.694 CPU sec, 4.537 real sec
sorting block 19, #reads = 1069789 mem = 536878771 peak = 1011003392
[M::mem_process_seqs] Processed 681402 reads in 94.619 CPU sec, 3.004 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 277723, 6, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 297, 607)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1479)
[M::mem_pestat] mean and std.dev: (368.48, 290.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1915)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 344, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (360.28, 172.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 279, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (261.43, 102.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 20, #reads = 1068725 mem = 536877264 peak = 1014968320
[M::process] read 682310 sequences (100000236 bp) in 2.476 CPU sec, 2.716 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 278718, 2, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 248, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (262.12, 116.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 341, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (357.98, 171.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 345, 573)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1289)
[M::mem_pestat] mean and std.dev: (357.79, 213.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1647)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685662 sequences (100000026 bp) in 1.380 CPU sec, 1.383 real sec...
[M::mem_process_seqs] Processed 681732 reads in 142.647 CPU sec, 4.528 real sec
[M::mem_process_seqs] Processed 681720 reads in 94.821 CPU sec, 3.010 real sec
sorting block 21, #reads = 1068457 mem = 536879910 peak = 1014968320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278587, 4, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 252, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (289.45, 156.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1212)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 344, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (361.17, 175.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 302, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1060)
[M::mem_pestat] mean and std.dev: (320.32, 178.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1354)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685022 sequences (100000291 bp) in 2.074 CPU sec, 2.151 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 278909, 5, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 196, 293)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (224.59, 91.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 695)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 322, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (340.97, 168.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 256, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (260.07, 100.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684596 sequences (100000008 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_process_seqs] Processed 682310 reads in 141.886 CPU sec, 4.528 real sec
[M::mem_process_seqs] Processed 685662 reads in 98.562 CPU sec, 3.151 real sec
sorting block 22, #reads = 1068374 mem = 536878766 peak = 1014968320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 278870, 6, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 266, 407)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (278.46, 166.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 324, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (343.02, 168.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 355, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (325.92, 130.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683956 sequences (100000034 bp) in 2.057 CPU sec, 2.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (44, 278879, 4, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (194, 301, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1154)
[M::mem_pestat] mean and std.dev: (304.59, 172.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1474)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 329, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (347.69, 170.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 284, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587)
[M::mem_pestat] mean and std.dev: (276.92, 97.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 708)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683288 sequences (100000254 bp) in 1.390 CPU sec, 1.393 real sec...
[M::mem_process_seqs] Processed 685022 reads in 140.204 CPU sec, 4.462 real sec
sorting block 23, #reads = 1074278 mem = 536880314 peak = 1014968320
[M::mem_process_seqs] Processed 684596 reads in 91.918 CPU sec, 2.923 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278649, 6, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (142, 220, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (253.97, 135.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 918)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 332, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (350.21, 171.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 286, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (347.54, 174.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 24, #reads = 1073115 mem = 536878232 peak = 1014968320
[M::process] read 682756 sequences (100000162 bp) in 2.291 CPU sec, 2.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 278583, 2, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (214, 359, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853)
[M::mem_pestat] mean and std.dev: (315.48, 138.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1066)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 338, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (355.37, 173.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (320, 349, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (198, 503)
[M::mem_pestat] mean and std.dev: (341.32, 54.62)
[M::mem_pestat] low and high boundaries for proper pairs: (123, 564)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682326 sequences (100000004 bp) in 1.440 CPU sec, 1.445 real sec...
[M::mem_process_seqs] Processed 683956 reads in 141.768 CPU sec, 4.509 real sec
[M::mem_process_seqs] Processed 683288 reads in 93.889 CPU sec, 2.983 real sec
sorting block 25, #reads = 1071910 mem = 536880923 peak = 1014968320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278229, 6, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (195, 310, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732)
[M::mem_pestat] mean and std.dev: (268.39, 98.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 911)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 343, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (359.48, 174.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 366, 778)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852)
[M::mem_pestat] mean and std.dev: (469.87, 410.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2389)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 681556 sequences (100000195 bp) in 1.908 CPU sec, 1.982 real sec...
[M::process] read 681226 sequences (100000011 bp) in 1.420 CPU sec, 1.429 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 278249, 9, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 275, 621)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1431)
[M::mem_pestat] mean and std.dev: (371.50, 197.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1836)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 344, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 994)
[M::mem_pestat] mean and std.dev: (359.11, 172.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 372, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (323.94, 130.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 999)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682756 reads in 142.716 CPU sec, 4.549 real sec
[M::mem_process_seqs] Processed 682326 reads in 94.728 CPU sec, 3.014 real sec
sorting block 26, #reads = 1070294 mem = 536877640 peak = 1014968320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 277987, 7, 56)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (253.68, 137.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 345, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (359.51, 170.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 337, 412)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (325.56, 136.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 680876 sequences (100000057 bp) in 1.981 CPU sec, 2.046 real sec...
[M::process] read 681090 sequences (100000057 bp) in 1.420 CPU sec, 1.415 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 277532, 11, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 275, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571)
[M::mem_pestat] mean and std.dev: (261.30, 81.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 696)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 346, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (359.93, 169.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (777, 777, 2005)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4461)
[M::mem_pestat] mean and std.dev: (1080.18, 681.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5689)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 371, 692)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1584)
[M::mem_pestat] mean and std.dev: (371.22, 184.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2030)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 681556 reads in 144.146 CPU sec, 4.587 real sec
[M::mem_process_seqs] Processed 681226 reads in 94.798 CPU sec, 3.020 real sec
sorting block 27, #reads = 1069326 mem = 536876524 peak = 1014968320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 277721, 5, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 268, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1125)
[M::mem_pestat] mean and std.dev: (300.11, 174.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1444)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 348, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (361.21, 169.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 274, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1211)
[M::mem_pestat] mean and std.dev: (299.79, 192.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1555)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683008 sequences (100000148 bp) in 2.237 CPU sec, 2.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278076, 9, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (147, 301, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (305.10, 190.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 343, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (357.48, 167.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 305, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (331.71, 153.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1108)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 688580 sequences (100000162 bp) in 1.620 CPU sec, 1.623 real sec...
[M::mem_process_seqs] Processed 680876 reads in 145.015 CPU sec, 4.606 real sec
sorting block 28, #reads = 1068597 mem = 536881495 peak = 1014968320
[M::mem_process_seqs] Processed 681090 reads in 95.573 CPU sec, 3.035 real sec
sorting block 29, #reads = 1067639 mem = 536879316 peak = 1047035904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278274, 2, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 214, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124)
[M::mem_pestat] mean and std.dev: (277.39, 175.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1447)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 335, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (351.67, 169.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1216)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (279, 331, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (355.49, 165.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 686328 sequences (100000014 bp) in 2.147 CPU sec, 2.223 real sec...
[M::process] read 684502 sequences (100000121 bp) in 1.430 CPU sec, 1.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 280969, 11, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 263)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473)
[M::mem_pestat] mean and std.dev: (210.19, 62.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 578)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 303, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (329.30, 168.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1140, 1140, 2097)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4011)
[M::mem_pestat] mean and std.dev: (1195.70, 522.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4968)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 262, 363)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (265.42, 104.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 897)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683008 reads in 146.735 CPU sec, 4.667 real sec
[M::mem_process_seqs] Processed 688580 reads in 97.958 CPU sec, 3.119 real sec
sorting block 30, #reads = 1065944 mem = 536878986 peak = 1047035904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 280468, 8, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 259, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685)
[M::mem_pestat] mean and std.dev: (238.08, 99.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 318, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (339.91, 170.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1229)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 299, 620)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1528)
[M::mem_pestat] mean and std.dev: (396.64, 348.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1982)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684038 sequences (100000126 bp) in 2.306 CPU sec, 2.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279976, 16, 36)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (241, 295, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (93, 463)
[M::mem_pestat] mean and std.dev: (260.79, 87.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 612)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 333, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (351.59, 173.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (417, 651, 1618)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4020)
[M::mem_pestat] mean and std.dev: (665.50, 535.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5221)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 374, 578)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1302)
[M::mem_pestat] mean and std.dev: (389.78, 183.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1664)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 683710 sequences (100000143 bp) in 1.450 CPU sec, 1.447 real sec...
[M::mem_process_seqs] Processed 686328 reads in 142.265 CPU sec, 4.518 real sec
[M::mem_process_seqs] Processed 684502 reads in 94.888 CPU sec, 3.012 real sec
sorting block 31, #reads = 1070228 mem = 536880391 peak = 1047035904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (49, 279416, 1, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 316, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (291.98, 139.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1339)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 337, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (354.22, 172.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 294, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1092)
[M::mem_pestat] mean and std.dev: (390.53, 246.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1375)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683086 sequences (100000016 bp) in 2.488 CPU sec, 2.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278692, 7, 69)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (193, 249, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159)
[M::mem_pestat] mean and std.dev: (327.32, 203.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1481)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 341, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (358.33, 175.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 408, 544)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174)
[M::mem_pestat] mean and std.dev: (380.88, 162.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1489)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682544 sequences (100000003 bp) in 1.450 CPU sec, 1.457 real sec...
[M::mem_process_seqs] Processed 684038 reads in 144.402 CPU sec, 4.581 real sec
sorting block 32, #reads = 1068134 mem = 536878703 peak = 1047035904
[M::mem_process_seqs] Processed 683710 reads in 97.337 CPU sec, 3.088 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 278516, 11, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 294, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859)
[M::mem_pestat] mean and std.dev: (259.93, 119.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 339, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (356.12, 172.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (449, 449, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (359, 584)
[M::mem_pestat] mean and std.dev: (455.43, 15.75)
[M::mem_pestat] low and high boundaries for proper pairs: (314, 629)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 284, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (274.00, 94.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 954)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 33, #reads = 1066883 mem = 536882325 peak = 1047035904
[M::process] read 682434 sequences (100000133 bp) in 2.397 CPU sec, 2.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278473, 10, 33)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 345, 1406)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3836)
[M::mem_pestat] mean and std.dev: (835.58, 976.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5051)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 341, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (357.55, 172.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (326, 496, 888)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2012)
[M::mem_pestat] mean and std.dev: (497.67, 325.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2574)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 319, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (332.37, 154.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683086 reads in 150.230 CPU sec, 4.775 real sec
[M::process] read 682644 sequences (100000171 bp) in 1.847 CPU sec, 1.863 real sec...
[M::mem_process_seqs] Processed 682544 reads in 100.984 CPU sec, 3.189 real sec
sorting block 34, #reads = 1065440 mem = 536879586 peak = 1047035904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (53, 278175, 11, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (156, 247, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095)
[M::mem_pestat] mean and std.dev: (278.29, 152.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1408)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 340, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (357.28, 171.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (301, 329, 940)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2218)
[M::mem_pestat] mean and std.dev: (517.40, 466.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2857)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 233, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (274.02, 127.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682368 sequences (100000003 bp) in 2.156 CPU sec, 2.221 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278380, 6, 38)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 193, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (230.29, 143.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 339, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (355.42, 170.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 363, 625)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1357)
[M::mem_pestat] mean and std.dev: (356.13, 190.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1723)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682120 sequences (100000221 bp) in 1.720 CPU sec, 1.720 real sec...
[M::mem_process_seqs] Processed 682434 reads in 150.015 CPU sec, 4.767 real sec
[M::mem_process_seqs] Processed 682644 reads in 101.163 CPU sec, 3.213 real sec
sorting block 35, #reads = 1065874 mem = 536879265 peak = 1047035904
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (36, 278405, 3, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (271, 334, 413)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697)
[M::mem_pestat] mean and std.dev: (297.91, 103.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 338, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (355.85, 171.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 309, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (315.15, 158.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 690478 sequences (100000189 bp) in 1.981 CPU sec, 2.055 real sec...
[M::process] read 689210 sequences (100000077 bp) in 1.490 CPU sec, 1.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 278467, 11, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 204, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426)
[M::mem_pestat] mean and std.dev: (200.57, 62.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 517)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 340, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (357.64, 172.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (432, 562, 1522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3702)
[M::mem_pestat] mean and std.dev: (903.00, 892.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4792)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 355, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832)
[M::mem_pestat] mean and std.dev: (327.12, 120.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682368 reads in 148.827 CPU sec, 4.733 real sec
[M::mem_process_seqs] Processed 682120 reads in 99.760 CPU sec, 3.178 real sec
sorting block 36, #reads = 1064749 mem = 536877783 peak = 1059717120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 281818, 2, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 197, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (254.57, 181.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 293, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (316.60, 159.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 389, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (320.91, 118.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687240 sequences (100000025 bp) in 1.927 CPU sec, 1.997 real sec...
[M::process] read 685700 sequences (100000034 bp) in 1.460 CPU sec, 1.455 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 281097, 3, 46)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 238, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (271.40, 138.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 300, 431)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (320.90, 158.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 329, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137)
[M::mem_pestat] mean and std.dev: (318.78, 168.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1447)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 690478 reads in 159.716 CPU sec, 5.087 real sec
sorting block 37, #reads = 1063442 mem = 536878683 peak = 1095540736
[M::mem_process_seqs] Processed 689210 reads in 106.464 CPU sec, 3.402 real sec
sorting block 38, #reads = 1062189 mem = 536879485 peak = 1095540736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 281348, 4, 47)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 288, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200)
[M::mem_pestat] mean and std.dev: (357.35, 271.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1542)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 312, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (331.37, 162.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706)
[M::mem_pestat] mean and std.dev: (246.87, 114.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 891)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685024 sequences (100000083 bp) in 1.987 CPU sec, 2.035 real sec...
[M::process] read 684346 sequences (100000267 bp) in 1.460 CPU sec, 1.467 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 280980, 1, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 259, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (250.48, 108.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 321, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (338.83, 164.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 216, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431)
[M::mem_pestat] mean and std.dev: (204.21, 63.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 522)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 687240 reads in 152.265 CPU sec, 4.847 real sec
[M::mem_process_seqs] Processed 685700 reads in 99.427 CPU sec, 3.163 real sec
sorting block 39, #reads = 1064491 mem = 536880042 peak = 1095540736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279978, 11, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 233, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (289.36, 159.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 328, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973)
[M::mem_pestat] mean and std.dev: (344.98, 168.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (625, 625, 1168)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2254)
[M::mem_pestat] mean and std.dev: (656.22, 386.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2797)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 263, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (303.12, 145.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 884)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 683248 sequences (100000008 bp) in 2.563 CPU sec, 2.758 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 279194, 8, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (172, 191, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691)
[M::mem_pestat] mean and std.dev: (257.70, 130.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 864)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 329, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (347.32, 169.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 311, 545)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1177)
[M::mem_pestat] mean and std.dev: (353.93, 170.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1493)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682538 sequences (100000068 bp) in 1.460 CPU sec, 1.454 real sec...
[M::mem_process_seqs] Processed 685024 reads in 150.477 CPU sec, 4.776 real sec
[M::mem_process_seqs] Processed 684346 reads in 100.864 CPU sec, 3.202 real sec
sorting block 40, #reads = 1066261 mem = 536881688 peak = 1095540736
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 279094, 5, 49)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (157, 263, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (243.71, 115.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 945)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 337, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (354.18, 171.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1251)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 378, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (339.93, 121.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 969)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682428 sequences (100000154 bp) in 1.987 CPU sec, 2.052 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 279004, 2, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (220, 303, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (312.05, 164.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 340, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (358.09, 173.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 271, 602)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1438)
[M::mem_pestat] mean and std.dev: (356.82, 240.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1856)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 682262 sequences (100000119 bp) in 1.580 CPU sec, 1.579 real sec...
[M::mem_process_seqs] Processed 683248 reads in 149.406 CPU sec, 4.754 real sec
sorting block 41, #reads = 1065272 mem = 536878042 peak = 1095540736
[M::mem_process_seqs] Processed 682538 reads in 98.788 CPU sec, 3.138 real sec
sorting block 42, #reads = 1065272 mem = 536880461 peak = 1120579584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 278814, 8, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (219, 360, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (327.81, 170.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1215)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 341, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (360.30, 175.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 291, 415)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (315.22, 163.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684748 sequences (100000263 bp) in 2.262 CPU sec, 2.320 real sec...
[M::process] read 688512 sequences (100000060 bp) in 1.580 CPU sec, 1.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 278922, 10, 54)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 330, 1410)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3858)
[M::mem_pestat] mean and std.dev: (648.67, 643.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5082)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 344, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (362.89, 178.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (290, 661, 867)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2021)
[M::mem_pestat] mean and std.dev: (529.11, 315.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2598)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 279, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (302.53, 146.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 682428 reads in 147.171 CPU sec, 4.668 real sec
[M::mem_process_seqs] Processed 682262 reads in 98.604 CPU sec, 3.134 real sec
sorting block 43, #reads = 1065702 mem = 536882277 peak = 1120579584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 279455, 12, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 292, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159)
[M::mem_pestat] mean and std.dev: (314.29, 171.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1490)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 327, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (349.44, 174.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (241, 352, 1389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3685)
[M::mem_pestat] mean and std.dev: (674.83, 545.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4833)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 305, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (323.11, 181.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 686372 sequences (100000204 bp) in 2.449 CPU sec, 2.632 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 281123, 2, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (215, 302, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716)
[M::mem_pestat] mean and std.dev: (266.28, 99.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 306, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (329.63, 166.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 296, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (269.83, 100.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684878 sequences (100000005 bp) in 1.450 CPU sec, 1.450 real sec...
[M::mem_process_seqs] Processed 684748 reads in 155.606 CPU sec, 4.950 real sec
[M::mem_process_seqs] Processed 688512 reads in 100.334 CPU sec, 3.186 real sec
sorting block 44, #reads = 1061409 mem = 536881096 peak = 1120579584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 280473, 3, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (145, 233, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868)
[M::mem_pestat] mean and std.dev: (233.19, 121.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1109)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 318, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (340.78, 171.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 255, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1191)
[M::mem_pestat] mean and std.dev: (332.59, 240.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1534)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684392 sequences (100000162 bp) in 2.355 CPU sec, 2.537 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 279655, 7, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 302, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824)
[M::mem_pestat] mean and std.dev: (311.34, 124.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1029)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 326, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (345.35, 170.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1220)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 323, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (356.12, 189.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683974 sequences (100000029 bp) in 1.460 CPU sec, 1.452 real sec...
[M::mem_process_seqs] Processed 686372 reads in 141.733 CPU sec, 4.499 real sec
sorting block 45, #reads = 1069954 mem = 536879128 peak = 1120579584
[M::mem_process_seqs] Processed 684878 reads in 93.241 CPU sec, 2.962 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 279099, 3, 25)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 262, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (294.25, 151.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 328, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (346.85, 169.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1222)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 318, 498)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076)
[M::mem_pestat] mean and std.dev: (331.82, 164.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1365)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684516 sequences (100000062 bp) in 2.474 CPU sec, 2.649 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (48, 279761, 4, 34)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (164, 201, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (237.53, 115.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 329, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (346.02, 167.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 255, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (264.84, 137.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684392 reads in 141.840 CPU sec, 4.505 real sec
sorting block 46, #reads = 1070720 mem = 536881930 peak = 1120579584
[M::process] read 684996 sequences (100000194 bp) in 1.684 CPU sec, 1.704 real sec...
[M::mem_process_seqs] Processed 683974 reads in 94.398 CPU sec, 2.982 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 280021, 4, 45)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 227, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687)
[M::mem_pestat] mean and std.dev: (229.21, 124.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 870)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 325, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (344.72, 168.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 268, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (265.93, 101.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 47, #reads = 1069906 mem = 536882021 peak = 1127002112
[M::process] read 683878 sequences (100000005 bp) in 2.475 CPU sec, 2.690 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 279700, 7, 41)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 184, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (205.31, 83.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 322, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (341.51, 168.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 298, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (308.75, 132.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 997)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 683170 sequences (100000109 bp) in 1.430 CPU sec, 1.433 real sec...
[M::mem_process_seqs] Processed 684516 reads in 147.113 CPU sec, 4.676 real sec
[M::mem_process_seqs] Processed 684996 reads in 97.438 CPU sec, 3.090 real sec
sorting block 48, #reads = 1067940 mem = 536877705 peak = 1127002112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 279145, 10, 57)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 345, 987)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2543)
[M::mem_pestat] mean and std.dev: (490.74, 540.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3321)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 335, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (353.88, 174.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (422, 422, 3813)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10595)
[M::mem_pestat] mean and std.dev: (1806.50, 2190.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13986)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 387, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (329.25, 110.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 683332 sequences (100000065 bp) in 1.943 CPU sec, 1.999 real sec...
[M::process] read 685840 sequences (100000227 bp) in 1.470 CPU sec, 1.466 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (45, 279308, 6, 39)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 282, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (311.69, 167.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1230)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 338, 478)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (357.02, 174.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 251, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1202)
[M::mem_pestat] mean and std.dev: (253.03, 136.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1549)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683878 reads in 146.951 CPU sec, 4.680 real sec
[M::mem_process_seqs] Processed 683170 reads in 98.445 CPU sec, 3.131 real sec
sorting block 49, #reads = 1066996 mem = 536879783 peak = 1127002112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 279250, 5, 43)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 270, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (305.60, 142.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 335, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (356.26, 176.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 377, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (401.24, 227.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 691510 sequences (100000249 bp) in 2.503 CPU sec, 2.686 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (40, 279919, 3, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 268, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740)
[M::mem_pestat] mean and std.dev: (235.35, 103.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 321, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (344.73, 174.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1236)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 383, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (347.12, 174.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1238)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 690566 sequences (100000100 bp) in 1.510 CPU sec, 1.505 real sec...
[M::mem_process_seqs] Processed 683332 reads in 155.483 CPU sec, 4.938 real sec
sorting block 50, #reads = 1066229 mem = 536880252 peak = 1127002112
[M::mem_process_seqs] Processed 685840 reads in 108.062 CPU sec, 3.434 real sec
sorting block 51, #reads = 1062120 mem = 536877906 peak = 1127002112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (47, 282125, 9, 52)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 202, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544)
[M::mem_pestat] mean and std.dev: (231.55, 99.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 671)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 288, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (311.42, 156.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 271, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (294.85, 171.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 687660 sequences (100000069 bp) in 2.120 CPU sec, 2.168 real sec...
[M::process] read 686312 sequences (100000218 bp) in 1.490 CPU sec, 1.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 282055, 2, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 285, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (286.58, 140.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 293, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (314.86, 156.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 246, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536)
[M::mem_pestat] mean and std.dev: (239.24, 82.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 653)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 691510 reads in 160.057 CPU sec, 5.097 real sec
[M::mem_process_seqs] Processed 690566 reads in 106.463 CPU sec, 3.388 real sec
sorting block 52, #reads = 1065124 mem = 536880362 peak = 1127002112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (39, 280651, 4, 28)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 286, 552)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1322)
[M::mem_pestat] mean and std.dev: (356.81, 255.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1707)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 309, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (327.51, 160.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (130, 308, 596)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1528)
[M::mem_pestat] mean and std.dev: (336.93, 249.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1994)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 685532 sequences (100000104 bp) in 2.510 CPU sec, 2.672 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 280234, 4, 31)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 235, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (275.39, 179.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 319, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (337.62, 165.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 254, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (302.14, 184.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684718 sequences (100000061 bp) in 1.470 CPU sec, 1.471 real sec...
[M::mem_process_seqs] Processed 687660 reads in 153.726 CPU sec, 4.881 real sec
[M::mem_process_seqs] Processed 686312 reads in 101.378 CPU sec, 3.221 real sec
sorting block 53, #reads = 1063170 mem = 536877831 peak = 1127002112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 280209, 8, 32)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (134, 172, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506)
[M::mem_pestat] mean and std.dev: (185.80, 71.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 630)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 324, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (342.30, 167.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 450, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1161)
[M::mem_pestat] mean and std.dev: (377.93, 183.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1480)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 684042 sequences (100000031 bp) in 2.414 CPU sec, 2.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (46, 279254, 12, 37)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 335, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (293.38, 129.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 328, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (346.39, 169.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1243)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (636, 656, 971)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1641)
[M::mem_pestat] mean and std.dev: (658.70, 216.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1976)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 385, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989)
[M::mem_pestat] mean and std.dev: (350.26, 182.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 682908 sequences (100000213 bp) in 1.460 CPU sec, 1.461 real sec...
[M::mem_process_seqs] Processed 685532 reads in 152.017 CPU sec, 4.828 real sec
sorting block 54, #reads = 1066431 mem = 536876262 peak = 1127002112
[M::mem_process_seqs] Processed 684718 reads in 100.936 CPU sec, 3.206 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 279014, 11, 40)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 196, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688)
[M::mem_pestat] mean and std.dev: (246.03, 150.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 877)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 332, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (348.69, 168.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1407, 2366, 2366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4284)
[M::mem_pestat] mean and std.dev: (1564.00, 932.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5293)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 251, 416)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (308.77, 164.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1064)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
sorting block 55, #reads = 1066582 mem = 536881264 peak = 1127002112
[M::process] read 682644 sequences (100000115 bp) in 2.364 CPU sec, 2.535 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 279135, 11, 42)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (196, 377, 631)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1501)
[M::mem_pestat] mean and std.dev: (345.20, 209.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1936)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 340, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (356.44, 171.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (654, 1227, 6041)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16815)
[M::mem_pestat] mean and std.dev: (2292.36, 2360.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 22202)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 250, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (289.85, 165.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 681926 sequences (100000015 bp) in 1.460 CPU sec, 1.459 real sec...
[M::mem_process_seqs] Processed 684042 reads in 150.639 CPU sec, 4.799 real sec
[M::mem_process_seqs] Processed 682908 reads in 99.330 CPU sec, 3.155 real sec
[M::process] read 66882 sequences (9771461 bp) in 0.422 CPU sec, 0.430 real sec...
sorting block 56, #reads = 1067663 mem = 536879564 peak = 1127002112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 278901, 14, 20)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 236, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1265)
[M::mem_pestat] mean and std.dev: (331.08, 266.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1633)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 339, 473)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (355.14, 170.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (329, 863, 1592)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4118)
[M::mem_pestat] mean and std.dev: (1128.43, 1088.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5481)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 430, 722)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1712)
[M::mem_pestat] mean and std.dev: (430.32, 263.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2207)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 278575, 5, 51)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 284, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (303.78, 163.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 342, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (358.29, 171.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 268, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535)
[M::mem_pestat] mean and std.dev: (234.36, 71.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 648)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 27272, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 327, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (344.91, 166.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682644 reads in 150.033 CPU sec, 4.872 real sec
[M::mem_process_seqs] Processed 681926 reads in 101.001 CPU sec, 3.273 real sec
[M::mem_process_seqs] Processed 66882 reads in 102.886 CPU sec, 3.342 real sec
sorting block 57, #reads = 1065628 mem = 536880952 peak = 1127002112
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 62291700
overall: 70101008384 mem 8369.199 user 276.404 sys 1246.013 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1029_N5D-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tLB:1029_N5D-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0002.1029_N5D-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 1246.045 sec; CPU: 8645.605 sec
sorting block 58, #reads = 705840 mem = 355491671 peak = 1145933824
uniq header count 1
merging 59 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 3939406196
output reads: 62657698
bam_mem_sort: 59 calls 19.409 user 0.316 sys 19.813 real
bam_write: 59 calls 66.663 user 1.735 sys 68.584 real
execute: 1 calls 15.362 user 0.894 sys 1269.459 real
merge_files: 1 calls 15.340 user 0.872 sys 22.369 real
parse_chunk: 939 calls 31.750 user 9.637 sys 41.938 real
read_chunk: 2537 calls 21.417 user 17.481 sys 249.053 real
sort_block: 1 calls 16.084 user 17.260 sys 240.827 real
write_chunk: 1596 calls 14.904 user 0.661 sys 21.496 real
overall: 2879905792 mem 820.533 user 34.208 sys 1269.480 real
ls: cannot access '*.cram': No such file or directory