This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_cVw-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1658.368 CPU sec, 60.248 real sec
[M::process] read 681418 sequences (100000029 bp) in 1.000 CPU sec, 1.001 real sec...
[M::process] read 680828 sequences (100000002 bp) in 1.500 CPU sec, 1.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284204, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 305, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (325.00, 152.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680426 sequences (100000235 bp) in 1.580 CPU sec, 1.588 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283808, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 312, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (330.50, 155.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679982 sequences (100000115 bp) in 1.520 CPU sec, 1.517 real sec...
[M::mem_process_seqs] Processed 681418 reads in 155.047 CPU sec, 4.861 real sec
[M::mem_process_seqs] Processed 680828 reads in 98.933 CPU sec, 3.135 real sec
sorting block 0, #reads = 1084579 mem = 536877225 peak = 590688256
[M::process] read 679608 sequences (100000157 bp) in 1.824 CPU sec, 2.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283951, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 315, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (333.94, 156.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679248 sequences (100000042 bp) in 1.530 CPU sec, 1.534 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283249, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 317, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (336.28, 157.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283518, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 321, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (338.23, 157.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680426 reads in 225.980 CPU sec, 7.220 real sec
[M::mem_process_seqs] Processed 679982 reads in 168.293 CPU sec, 5.412 real sec
[M::mem_process_seqs] Processed 679608 reads in 183.443 CPU sec, 5.920 real sec
sorting block 1, #reads = 1084225 mem = 536878586 peak = 722808832
sorting block 2, #reads = 1084564 mem = 536879857 peak = 837918720
[M::process] read 679164 sequences (100000286 bp) in 2.186 CPU sec, 2.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283536, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 324, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (342.32, 159.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679010 sequences (100000261 bp) in 1.820 CPU sec, 1.841 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283218, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 325, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (343.27, 160.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679248 reads in 154.101 CPU sec, 4.914 real sec
[M::mem_process_seqs] Processed 679164 reads in 99.988 CPU sec, 3.164 real sec
[M::process] read 678656 sequences (100000218 bp) in 2.084 CPU sec, 2.162 real sec...
sorting block 3, #reads = 1084503 mem = 536880744 peak = 837918720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282717, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (344.57, 160.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678564 sequences (100000216 bp) in 2.200 CPU sec, 2.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282747, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (347.19, 161.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679010 reads in 154.516 CPU sec, 4.904 real sec
[M::process] read 678394 sequences (100000147 bp) in 1.605 CPU sec, 1.614 real sec...
[M::mem_process_seqs] Processed 678656 reads in 98.674 CPU sec, 3.113 real sec
sorting block 4, #reads = 1084061 mem = 536881251 peak = 837918720
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283085, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (347.40, 161.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678292 sequences (100000176 bp) in 2.190 CPU sec, 2.272 real sec...
[M::process] read 677958 sequences (100000004 bp) in 1.500 CPU sec, 1.497 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282606, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (348.19, 161.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678564 reads in 148.769 CPU sec, 4.722 real sec
sorting block 5, #reads = 1084053 mem = 536878813 peak = 837918720
[M::mem_process_seqs] Processed 678394 reads in 94.653 CPU sec, 2.993 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282701, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (348.93, 162.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678030 sequences (100000274 bp) in 2.230 CPU sec, 2.324 real sec...
[M::process] read 677808 sequences (100000093 bp) in 1.667 CPU sec, 1.674 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282640, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (350.38, 162.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678292 reads in 152.150 CPU sec, 4.830 real sec
sorting block 6, #reads = 1082991 mem = 536881488 peak = 837918720
[M::mem_process_seqs] Processed 677958 reads in 99.796 CPU sec, 3.177 real sec
sorting block 7, #reads = 1083812 mem = 536879351 peak = 871825408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283114, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (351.89, 163.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677654 sequences (100000116 bp) in 2.357 CPU sec, 2.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282572, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677424 sequences (100000226 bp) in 1.550 CPU sec, 1.547 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (221, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (350.68, 162.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678030 reads in 159.117 CPU sec, 5.069 real sec
[M::mem_process_seqs] Processed 677808 reads in 101.154 CPU sec, 3.215 real sec
sorting block 8, #reads = 1084487 mem = 536878544 peak = 871825408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282193, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (350.46, 161.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677864 sequences (100000241 bp) in 2.565 CPU sec, 2.724 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281932, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (351.75, 163.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677896 sequences (100000231 bp) in 1.718 CPU sec, 1.723 real sec...
[M::mem_process_seqs] Processed 677654 reads in 160.602 CPU sec, 5.107 real sec
[M::mem_process_seqs] Processed 677424 reads in 102.918 CPU sec, 3.260 real sec
sorting block 9, #reads = 1083100 mem = 536880303 peak = 871825408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282687, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 332, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (350.19, 162.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678044 sequences (100000126 bp) in 2.215 CPU sec, 2.329 real sec...
[M::process] read 678234 sequences (100000218 bp) in 1.730 CPU sec, 1.730 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282306, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 329, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (348.02, 161.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677864 reads in 162.812 CPU sec, 5.179 real sec
[M::mem_process_seqs] Processed 677896 reads in 104.333 CPU sec, 3.309 real sec
sorting block 10, #reads = 1082789 mem = 536878818 peak = 871825408
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283095, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (346.74, 160.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678098 sequences (100000025 bp) in 2.529 CPU sec, 2.644 real sec...
[M::process] read 678198 sequences (100000210 bp) in 1.590 CPU sec, 1.590 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282095, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 327, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (345.09, 159.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678044 reads in 162.711 CPU sec, 5.177 real sec
sorting block 11, #reads = 1083638 mem = 536877642 peak = 871825408
[M::mem_process_seqs] Processed 678234 reads in 102.330 CPU sec, 3.246 real sec
sorting block 12, #reads = 1083800 mem = 536881021 peak = 918097920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282167, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (345.82, 160.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677840 sequences (100000253 bp) in 2.436 CPU sec, 2.539 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282195, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (345.47, 160.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677770 sequences (100000274 bp) in 1.830 CPU sec, 1.827 real sec...
[M::mem_process_seqs] Processed 678098 reads in 162.652 CPU sec, 5.167 real sec
[M::mem_process_seqs] Processed 678198 reads in 102.814 CPU sec, 3.254 real sec
sorting block 13, #reads = 1082782 mem = 536881651 peak = 918097920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282964, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (347.69, 160.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677614 sequences (100000255 bp) in 2.586 CPU sec, 2.707 real sec...
[M::process] read 677718 sequences (100000294 bp) in 1.640 CPU sec, 1.644 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282443, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (347.97, 160.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677840 reads in 163.164 CPU sec, 5.188 real sec
[M::mem_process_seqs] Processed 677770 reads in 102.065 CPU sec, 3.236 real sec
sorting block 14, #reads = 1082501 mem = 536879283 peak = 918097920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282196, 7, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (348.02, 160.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677528 sequences (100000152 bp) in 1.970 CPU sec, 2.062 real sec...
[M::process] read 677266 sequences (100000208 bp) in 1.436 CPU sec, 1.443 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282238, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (348.13, 160.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677614 reads in 162.667 CPU sec, 5.196 real sec
[M::mem_process_seqs] Processed 677718 reads in 101.119 CPU sec, 3.219 real sec
sorting block 15, #reads = 1083292 mem = 536879243 peak = 918097920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282386, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (347.94, 160.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676528 sequences (100000261 bp) in 3.017 CPU sec, 3.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281941, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (350.40, 160.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677528 reads in 160.271 CPU sec, 5.083 real sec
sorting block 16, #reads = 1083203 mem = 536876001 peak = 918097920
[M::process] read 677356 sequences (100000137 bp) in 1.946 CPU sec, 1.973 real sec...
[M::mem_process_seqs] Processed 677266 reads in 101.999 CPU sec, 3.215 real sec
sorting block 17, #reads = 1082844 mem = 536880295 peak = 937918464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281546, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 339, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (354.59, 161.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677216 sequences (100000114 bp) in 2.168 CPU sec, 2.276 real sec...
[M::process] read 677218 sequences (100000148 bp) in 1.420 CPU sec, 1.421 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281734, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (348.07, 159.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676528 reads in 158.219 CPU sec, 5.042 real sec
[M::mem_process_seqs] Processed 677356 reads in 101.139 CPU sec, 3.215 real sec
sorting block 18, #reads = 1083030 mem = 536875179 peak = 937918464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282447, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (348.61, 159.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677176 sequences (100000178 bp) in 2.356 CPU sec, 2.464 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282048, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (348.62, 158.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676950 sequences (100000145 bp) in 1.480 CPU sec, 1.482 real sec...
[M::mem_process_seqs] Processed 677216 reads in 152.564 CPU sec, 4.849 real sec
[M::mem_process_seqs] Processed 677218 reads in 96.347 CPU sec, 3.049 real sec
sorting block 19, #reads = 1083440 mem = 536879741 peak = 937918464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282201, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (346.27, 157.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676684 sequences (100000110 bp) in 2.055 CPU sec, 2.155 real sec...
[M::process] read 676854 sequences (100000242 bp) in 1.340 CPU sec, 1.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282275, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (349.93, 159.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677176 reads in 140.082 CPU sec, 4.459 real sec
sorting block 20, #reads = 1083883 mem = 536880080 peak = 937918464
[M::mem_process_seqs] Processed 676950 reads in 89.480 CPU sec, 2.841 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282269, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (351.06, 160.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 21, #reads = 1082915 mem = 536878711 peak = 937918464
[M::process] read 676408 sequences (100000207 bp) in 2.267 CPU sec, 2.362 real sec...
[M::process] read 676400 sequences (100000215 bp) in 1.420 CPU sec, 1.413 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282044, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (352.07, 161.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676684 reads in 142.141 CPU sec, 4.512 real sec
[M::mem_process_seqs] Processed 676854 reads in 89.954 CPU sec, 2.849 real sec
sorting block 22, #reads = 1082848 mem = 536879396 peak = 937918464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281971, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 339, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (355.85, 162.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676390 sequences (100000064 bp) in 2.021 CPU sec, 2.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282617, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 679688 sequences (100000018 bp) in 1.490 CPU sec, 1.491 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (229, 342, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (358.20, 163.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676408 reads in 142.066 CPU sec, 4.514 real sec
[M::mem_process_seqs] Processed 676400 reads in 89.934 CPU sec, 2.847 real sec
sorting block 23, #reads = 1082792 mem = 536876866 peak = 937918464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281872, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 339, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (355.92, 162.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680562 sequences (100000129 bp) in 2.593 CPU sec, 2.674 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283432, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 320, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (339.23, 159.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680022 sequences (100000165 bp) in 1.399 CPU sec, 1.395 real sec...
[M::mem_process_seqs] Processed 676390 reads in 143.918 CPU sec, 4.557 real sec
[M::mem_process_seqs] Processed 679688 reads in 93.343 CPU sec, 2.953 real sec
sorting block 24, #reads = 1083043 mem = 536879546 peak = 937918464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283702, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 316, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (335.47, 158.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679718 sequences (100000072 bp) in 2.098 CPU sec, 2.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283068, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 318, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (337.99, 159.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679368 sequences (100000235 bp) in 1.430 CPU sec, 1.431 real sec...
[M::mem_process_seqs] Processed 680562 reads in 141.513 CPU sec, 4.504 real sec
sorting block 25, #reads = 1083333 mem = 536879260 peak = 937918464
[M::mem_process_seqs] Processed 680022 reads in 90.525 CPU sec, 2.859 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283268, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 323, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (341.91, 161.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 26, #reads = 1085375 mem = 536880258 peak = 937918464
[M::process] read 679314 sequences (100000087 bp) in 2.288 CPU sec, 2.382 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283685, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 325, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (343.19, 161.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679284 sequences (100000294 bp) in 1.449 CPU sec, 1.443 real sec...
[M::mem_process_seqs] Processed 679718 reads in 137.825 CPU sec, 4.374 real sec
[M::mem_process_seqs] Processed 679368 reads in 87.443 CPU sec, 2.766 real sec
sorting block 27, #reads = 1086245 mem = 536877401 peak = 937918464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283578, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 327, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (345.18, 162.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678700 sequences (100000274 bp) in 2.341 CPU sec, 2.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282982, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (345.73, 162.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679314 reads in 138.483 CPU sec, 4.396 real sec
[M::process] read 678494 sequences (100000036 bp) in 1.720 CPU sec, 1.745 real sec...
[M::mem_process_seqs] Processed 679284 reads in 87.792 CPU sec, 2.762 real sec
sorting block 28, #reads = 1086433 mem = 536876829 peak = 937918464
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282236, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (347.42, 162.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678608 sequences (100000100 bp) in 2.538 CPU sec, 2.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283292, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (349.01, 163.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678700 reads in 131.978 CPU sec, 4.197 real sec
[M::mem_process_seqs] Processed 678494 reads in 84.469 CPU sec, 2.656 real sec
sorting block 29, #reads = 1084185 mem = 536878750 peak = 937918464
[M::process] read 678444 sequences (100000254 bp) in 2.222 CPU sec, 2.295 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282583, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (350.42, 163.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678372 sequences (100000285 bp) in 2.037 CPU sec, 2.136 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282527, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (351.38, 163.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678608 reads in 131.161 CPU sec, 4.171 real sec
[M::mem_process_seqs] Processed 678444 reads in 83.578 CPU sec, 2.632 real sec
sorting block 30, #reads = 1084024 mem = 536880939 peak = 937918464
[M::process] read 678042 sequences (100000209 bp) in 1.802 CPU sec, 1.860 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282648, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 335, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (351.20, 163.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 31, #reads = 1084761 mem = 536876284 peak = 942063616
[M::process] read 677674 sequences (100000214 bp) in 2.139 CPU sec, 2.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283077, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 336, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (352.42, 164.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678372 reads in 132.048 CPU sec, 4.185 real sec
[M::mem_process_seqs] Processed 678042 reads in 84.680 CPU sec, 2.663 real sec
[M::process] read 677788 sequences (100000279 bp) in 1.889 CPU sec, 1.927 real sec...
sorting block 32, #reads = 1084593 mem = 536878993 peak = 942063616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282527, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (354.24, 164.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677694 sequences (100000075 bp) in 1.861 CPU sec, 1.960 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282606, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (354.18, 165.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677488 sequences (100000106 bp) in 1.290 CPU sec, 1.287 real sec...
[M::mem_process_seqs] Processed 677674 reads in 133.662 CPU sec, 4.257 real sec
[M::mem_process_seqs] Processed 677788 reads in 85.182 CPU sec, 2.690 real sec
sorting block 33, #reads = 1084387 mem = 536877300 peak = 942063616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281744, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (354.55, 165.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677654 sequences (100000188 bp) in 2.517 CPU sec, 2.625 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282600, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (354.60, 165.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677694 reads in 133.893 CPU sec, 4.255 real sec
[M::mem_process_seqs] Processed 677488 reads in 85.272 CPU sec, 2.683 real sec
[M::process] read 677822 sequences (100000274 bp) in 2.046 CPU sec, 2.121 real sec...
sorting block 34, #reads = 1084422 mem = 536882544 peak = 942063616
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282160, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 337, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (354.09, 164.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677766 sequences (100000173 bp) in 1.982 CPU sec, 2.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282399, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (351.08, 163.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677654 reads in 132.685 CPU sec, 4.229 real sec
[M::mem_process_seqs] Processed 677822 reads in 84.655 CPU sec, 2.661 real sec
sorting block 35, #reads = 1083410 mem = 536880637 peak = 942063616
[M::process] read 678084 sequences (100000161 bp) in 2.014 CPU sec, 2.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282212, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (349.45, 162.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 36, #reads = 1083626 mem = 536878195 peak = 960339968
[M::process] read 678236 sequences (100000085 bp) in 2.315 CPU sec, 2.438 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282374, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (350.34, 164.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677766 reads in 131.086 CPU sec, 4.165 real sec
[M::mem_process_seqs] Processed 678084 reads in 82.685 CPU sec, 2.603 real sec
[M::process] read 678236 sequences (100000269 bp) in 1.718 CPU sec, 1.760 real sec...
sorting block 37, #reads = 1083061 mem = 536878261 peak = 960339968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282489, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (350.14, 163.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678138 sequences (100000264 bp) in 1.845 CPU sec, 1.932 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282755, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (348.60, 163.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678022 sequences (100000083 bp) in 1.290 CPU sec, 1.287 real sec...
[M::mem_process_seqs] Processed 678236 reads in 129.644 CPU sec, 4.116 real sec
[M::mem_process_seqs] Processed 678236 reads in 82.714 CPU sec, 2.612 real sec
sorting block 38, #reads = 1083915 mem = 536879274 peak = 960339968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282811, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (349.15, 162.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677982 sequences (100000200 bp) in 2.417 CPU sec, 2.575 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282387, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (348.18, 162.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678138 reads in 130.962 CPU sec, 4.153 real sec
[M::process] read 677928 sequences (100000165 bp) in 1.299 CPU sec, 1.299 real sec...
[M::mem_process_seqs] Processed 678022 reads in 82.033 CPU sec, 2.592 real sec
sorting block 39, #reads = 1084003 mem = 536880635 peak = 960339968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282372, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 329, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (348.00, 162.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677672 sequences (100000045 bp) in 1.868 CPU sec, 1.942 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282058, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677722 sequences (100000285 bp) in 1.300 CPU sec, 1.295 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (348.61, 162.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677982 reads in 127.180 CPU sec, 4.039 real sec
sorting block 40, #reads = 1083797 mem = 536878559 peak = 960339968
[M::mem_process_seqs] Processed 677928 reads in 79.660 CPU sec, 2.518 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282806, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (348.97, 162.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1083774 mem = 536876858 peak = 967737344
[M::process] read 677546 sequences (100000294 bp) in 2.087 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282414, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677582 sequences (100000008 bp) in 1.330 CPU sec, 1.324 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (220, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (348.30, 161.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677672 reads in 130.882 CPU sec, 4.154 real sec
[M::mem_process_seqs] Processed 677722 reads in 82.998 CPU sec, 2.627 real sec
sorting block 42, #reads = 1083047 mem = 536878541 peak = 967737344
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282500, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (349.53, 162.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677692 sequences (100000192 bp) in 2.231 CPU sec, 2.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282580, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (352.05, 163.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677546 reads in 129.541 CPU sec, 4.118 real sec
[M::process] read 677506 sequences (100000147 bp) in 1.950 CPU sec, 2.003 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282509, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (351.26, 163.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677692 reads in 72.986 CPU sec, 2.300 real sec
sorting block 43, #reads = 1083933 mem = 536881105 peak = 967737344
[M::process] read 677446 sequences (100000274 bp) in 2.007 CPU sec, 2.030 real sec...
[M::mem_process_seqs] Processed 677582 reads in 188.968 CPU sec, 5.958 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282515, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 335, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (352.41, 163.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 44, #reads = 1081819 mem = 536878902 peak = 1025105920
[M::process] read 677568 sequences (100000108 bp) in 2.168 CPU sec, 2.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282942, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 335, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (352.79, 163.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677506 reads in 141.527 CPU sec, 4.467 real sec
[M::mem_process_seqs] Processed 677446 reads in 82.756 CPU sec, 2.609 real sec
[M::process] read 676968 sequences (100000142 bp) in 1.927 CPU sec, 2.009 real sec...
sorting block 45, #reads = 1082380 mem = 536881202 peak = 1025105920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282562, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (349.61, 161.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677110 sequences (100000094 bp) in 1.816 CPU sec, 1.901 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282320, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (351.76, 161.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676728 sequences (100000272 bp) in 1.240 CPU sec, 1.245 real sec...
[M::mem_process_seqs] Processed 677568 reads in 128.364 CPU sec, 4.074 real sec
[M::mem_process_seqs] Processed 676968 reads in 81.161 CPU sec, 2.568 real sec
sorting block 46, #reads = 1083049 mem = 536879228 peak = 1025105920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282339, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 335, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (352.43, 162.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676594 sequences (100000255 bp) in 1.947 CPU sec, 2.050 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282787, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (354.26, 162.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676612 sequences (100000015 bp) in 1.310 CPU sec, 1.312 real sec...
[M::mem_process_seqs] Processed 677110 reads in 127.266 CPU sec, 4.039 real sec
sorting block 47, #reads = 1082357 mem = 536885068 peak = 1025105920
[M::mem_process_seqs] Processed 676728 reads in 79.766 CPU sec, 2.526 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282263, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 340, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (357.21, 164.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 48, #reads = 1082145 mem = 536880266 peak = 1025105920
[M::process] read 678222 sequences (100000124 bp) in 1.894 CPU sec, 1.980 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282489, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 343, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (359.39, 166.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681142 sequences (100000151 bp) in 1.340 CPU sec, 1.334 real sec...
[M::mem_process_seqs] Processed 676594 reads in 127.180 CPU sec, 4.036 real sec
[M::mem_process_seqs] Processed 676612 reads in 80.383 CPU sec, 2.541 real sec
sorting block 49, #reads = 1081321 mem = 536881427 peak = 1025105920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282576, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (350.46, 163.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680444 sequences (100000194 bp) in 1.901 CPU sec, 1.982 real sec...
[M::process] read 680196 sequences (100000062 bp) in 1.340 CPU sec, 1.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283594, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 312, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (331.51, 156.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678222 reads in 131.869 CPU sec, 4.190 real sec
[M::mem_process_seqs] Processed 681142 reads in 84.807 CPU sec, 2.684 real sec
sorting block 50, #reads = 1082039 mem = 536880141 peak = 1025105920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283949, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 315, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (334.15, 156.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679996 sequences (100000228 bp) in 2.543 CPU sec, 2.623 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283096, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 316, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (334.56, 156.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1119)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680444 reads in 131.095 CPU sec, 4.143 real sec
[M::mem_process_seqs] Processed 680196 reads in 82.177 CPU sec, 2.584 real sec
[M::process] read 679602 sequences (100000294 bp) in 1.974 CPU sec, 2.000 real sec...
sorting block 51, #reads = 1084643 mem = 536879390 peak = 1025105920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283205, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 318, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895)
[M::mem_pestat] mean and std.dev: (335.53, 155.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679340 sequences (100000090 bp) in 1.909 CPU sec, 1.999 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283076, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 322, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (339.32, 157.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679996 reads in 129.375 CPU sec, 4.110 real sec
[M::process] read 678948 sequences (100000137 bp) in 1.410 CPU sec, 1.412 real sec...
sorting block 52, #reads = 1084679 mem = 536878761 peak = 1025105920
[M::mem_process_seqs] Processed 679602 reads in 81.709 CPU sec, 2.578 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283425, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 324, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (340.95, 158.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1085198 mem = 536879844 peak = 1025105920
[M::process] read 678706 sequences (100000180 bp) in 2.254 CPU sec, 2.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283380, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 326, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (342.34, 158.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679340 reads in 125.607 CPU sec, 3.974 real sec
[M::process] read 678476 sequences (100000187 bp) in 1.420 CPU sec, 1.417 real sec...
[M::mem_process_seqs] Processed 678948 reads in 78.473 CPU sec, 2.474 real sec
sorting block 54, #reads = 1083801 mem = 536881046 peak = 1025105920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283499, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 328, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (343.41, 157.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678324 sequences (100000117 bp) in 2.013 CPU sec, 2.091 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283193, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 328, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (344.18, 157.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678706 reads in 124.546 CPU sec, 3.947 real sec
[M::mem_process_seqs] Processed 678476 reads in 80.059 CPU sec, 2.513 real sec
[M::process] read 678286 sequences (100000249 bp) in 1.930 CPU sec, 1.955 real sec...
sorting block 55, #reads = 1084514 mem = 536881324 peak = 1025105920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282753, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 329, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (344.60, 157.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678012 sequences (100000294 bp) in 2.397 CPU sec, 2.479 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283274, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (346.41, 158.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678324 reads in 130.052 CPU sec, 4.128 real sec
[M::mem_process_seqs] Processed 678286 reads in 83.264 CPU sec, 2.616 real sec
sorting block 56, #reads = 1082924 mem = 536879406 peak = 1025105920
[M::process] read 677920 sequences (100000280 bp) in 2.092 CPU sec, 2.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282889, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 332, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (347.96, 159.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677610 sequences (100000025 bp) in 2.144 CPU sec, 2.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282398, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (349.12, 160.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678012 reads in 134.120 CPU sec, 4.262 real sec
[M::mem_process_seqs] Processed 677920 reads in 86.040 CPU sec, 2.705 real sec
sorting block 57, #reads = 1082675 mem = 536877188 peak = 1025105920
[M::process] read 677508 sequences (100000057 bp) in 2.093 CPU sec, 2.149 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282404, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 336, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (351.59, 160.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1083597 mem = 536881950 peak = 1025105920
[M::process] read 677598 sequences (100000233 bp) in 2.269 CPU sec, 2.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282101, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 337, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (353.26, 161.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677610 reads in 133.509 CPU sec, 4.234 real sec
[M::mem_process_seqs] Processed 677508 reads in 83.749 CPU sec, 2.634 real sec
[M::process] read 677482 sequences (100000097 bp) in 1.607 CPU sec, 1.654 real sec...
sorting block 59, #reads = 1082632 mem = 536880473 peak = 1025105920
[M::process] read 677648 sequences (100000114 bp) in 2.109 CPU sec, 2.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282676, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 336, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (351.64, 160.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677482 reads in 72.458 CPU sec, 2.270 real sec
[M::process] read 677514 sequences (100000274 bp) in 1.792 CPU sec, 1.829 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282211, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 336, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (351.70, 161.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282609, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 336, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (352.75, 162.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677552 sequences (100000201 bp) in 1.436 CPU sec, 1.438 real sec...
[M::mem_process_seqs] Processed 677598 reads in 206.441 CPU sec, 6.543 real sec
sorting block 60, #reads = 1083320 mem = 536877788 peak = 1025105920
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282480, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (350.45, 160.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677514 reads in 72.612 CPU sec, 2.287 real sec
[M::process] read 677722 sequences (100000259 bp) in 2.037 CPU sec, 2.044 real sec...
sorting block 61, #reads = 1082227 mem = 536879431 peak = 1076957184
[M::mem_process_seqs] Processed 677648 reads in 187.429 CPU sec, 5.933 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282260, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (350.30, 162.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677720 sequences (100000082 bp) in 2.499 CPU sec, 2.599 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282659, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (347.68, 160.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677552 reads in 142.876 CPU sec, 4.516 real sec
[M::mem_process_seqs] Processed 677722 reads in 83.894 CPU sec, 2.642 real sec
sorting block 62, #reads = 1083090 mem = 536880029 peak = 1076957184
[M::process] read 677886 sequences (100000268 bp) in 1.817 CPU sec, 1.882 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281947, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (348.54, 161.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 63, #reads = 1082077 mem = 536878369 peak = 1076957184
[M::process] read 677658 sequences (100000068 bp) in 1.979 CPU sec, 2.062 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282846, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (349.22, 161.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677824 sequences (100000024 bp) in 1.270 CPU sec, 1.269 real sec...
[M::mem_process_seqs] Processed 677720 reads in 130.452 CPU sec, 4.135 real sec
[M::mem_process_seqs] Processed 677886 reads in 81.909 CPU sec, 2.586 real sec
sorting block 64, #reads = 1082251 mem = 536876964 peak = 1076957184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281856, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (349.24, 161.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677658 sequences (100000142 bp) in 2.112 CPU sec, 2.209 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282172, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 329, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (347.13, 160.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677528 sequences (100000000 bp) in 1.290 CPU sec, 1.285 real sec...
[M::mem_process_seqs] Processed 677658 reads in 127.574 CPU sec, 4.049 real sec
[M::mem_process_seqs] Processed 677824 reads in 81.810 CPU sec, 2.586 real sec
sorting block 65, #reads = 1082463 mem = 536879319 peak = 1076957184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281893, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (347.90, 160.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677730 sequences (100000118 bp) in 2.098 CPU sec, 2.174 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282192, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (349.23, 161.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677540 sequences (100000147 bp) in 1.310 CPU sec, 1.310 real sec...
[M::mem_process_seqs] Processed 677658 reads in 129.040 CPU sec, 4.094 real sec
[M::mem_process_seqs] Processed 677528 reads in 81.614 CPU sec, 2.582 real sec
sorting block 66, #reads = 1082692 mem = 536879498 peak = 1076957184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282345, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (349.91, 161.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677398 sequences (100000108 bp) in 2.224 CPU sec, 2.312 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282181, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (350.64, 162.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677410 sequences (100000131 bp) in 1.290 CPU sec, 1.294 real sec...
[M::mem_process_seqs] Processed 677730 reads in 129.008 CPU sec, 4.092 real sec
sorting block 67, #reads = 1083784 mem = 536877648 peak = 1076957184
[M::mem_process_seqs] Processed 677540 reads in 81.383 CPU sec, 2.572 real sec
sorting block 68, #reads = 1082911 mem = 536880306 peak = 1076957184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281900, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (351.04, 162.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677478 sequences (100000294 bp) in 2.424 CPU sec, 2.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282501, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (351.44, 162.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677398 reads in 126.560 CPU sec, 4.003 real sec
[M::mem_process_seqs] Processed 677410 reads in 79.568 CPU sec, 2.495 real sec
[M::process] read 677304 sequences (100000166 bp) in 1.896 CPU sec, 1.925 real sec...
sorting block 69, #reads = 1082790 mem = 536880025 peak = 1076957184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281831, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (351.99, 162.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676970 sequences (100000194 bp) in 1.870 CPU sec, 1.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282120, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (352.88, 163.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676910 sequences (100000149 bp) in 1.310 CPU sec, 1.305 real sec...
[M::mem_process_seqs] Processed 677478 reads in 125.044 CPU sec, 3.967 real sec
[M::mem_process_seqs] Processed 677304 reads in 77.575 CPU sec, 2.451 real sec
sorting block 70, #reads = 1083505 mem = 536879067 peak = 1076957184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282332, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (354.44, 163.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676838 sequences (100000255 bp) in 2.122 CPU sec, 2.198 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282046, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 337, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (354.55, 163.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676756 sequences (100000077 bp) in 1.300 CPU sec, 1.302 real sec...
[M::mem_process_seqs] Processed 676970 reads in 120.493 CPU sec, 3.819 real sec
[M::mem_process_seqs] Processed 676910 reads in 75.583 CPU sec, 2.381 real sec
sorting block 71, #reads = 1083535 mem = 536877241 peak = 1076957184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282519, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 339, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (356.64, 164.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676540 sequences (100000216 bp) in 1.983 CPU sec, 2.074 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282534, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 341, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (357.42, 164.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676278 sequences (100000024 bp) in 1.280 CPU sec, 1.278 real sec...
[M::mem_process_seqs] Processed 676838 reads in 117.537 CPU sec, 3.724 real sec
sorting block 72, #reads = 1082488 mem = 536877495 peak = 1076957184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282367, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (355.81, 163.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678228 sequences (100000083 bp) in 1.499 CPU sec, 1.504 real sec...
[M::mem_process_seqs] Processed 676540 reads in 67.886 CPU sec, 2.318 real sec
sorting block 73, #reads = 1083120 mem = 536881242 peak = 1076957184
[M::mem_process_seqs] Processed 676756 reads in 157.474 CPU sec, 5.151 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282407, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 340, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (357.55, 164.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680952 sequences (100000105 bp) in 2.184 CPU sec, 2.286 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283005, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (348.75, 162.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676278 reads in 130.957 CPU sec, 4.153 real sec
[M::mem_process_seqs] Processed 678228 reads in 77.461 CPU sec, 2.443 real sec
sorting block 74, #reads = 1082126 mem = 536877016 peak = 1076957184
[M::process] read 680554 sequences (100000299 bp) in 1.939 CPU sec, 2.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283660, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 313, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (334.15, 159.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 75, #reads = 1082890 mem = 536877988 peak = 1076957184
[M::process] read 680318 sequences (100000095 bp) in 1.766 CPU sec, 1.859 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282915, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 317, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (337.86, 161.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680952 reads in 123.080 CPU sec, 3.913 real sec
[M::mem_process_seqs] Processed 680554 reads in 77.683 CPU sec, 2.445 real sec
[M::process] read 679902 sequences (100000039 bp) in 1.564 CPU sec, 1.585 real sec...
sorting block 76, #reads = 1086083 mem = 536877569 peak = 1076957184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283064, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 319, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (340.10, 162.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679626 sequences (100000049 bp) in 2.296 CPU sec, 2.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283481, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 323, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (342.14, 162.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680318 reads in 123.697 CPU sec, 3.931 real sec
[M::mem_process_seqs] Processed 679902 reads in 77.568 CPU sec, 2.445 real sec
[M::process] read 679452 sequences (100000118 bp) in 1.645 CPU sec, 1.679 real sec...
sorting block 77, #reads = 1084865 mem = 536879090 peak = 1076957184
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282898, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 324, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (343.24, 162.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679310 sequences (100000268 bp) in 1.801 CPU sec, 1.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282228, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 325, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (344.04, 162.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679358 sequences (100000004 bp) in 1.340 CPU sec, 1.344 real sec...
[M::mem_process_seqs] Processed 679626 reads in 126.831 CPU sec, 4.033 real sec
[M::mem_process_seqs] Processed 679452 reads in 79.981 CPU sec, 2.523 real sec
sorting block 78, #reads = 1085179 mem = 536883184 peak = 1082691584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 282686, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (345.68, 162.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678976 sequences (100000041 bp) in 2.492 CPU sec, 2.622 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282443, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (345.45, 163.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679310 reads in 126.168 CPU sec, 4.008 real sec
[M::mem_process_seqs] Processed 679358 reads in 79.309 CPU sec, 2.501 real sec
sorting block 79, #reads = 1084436 mem = 536880963 peak = 1082691584
[M::process] read 678690 sequences (100000213 bp) in 1.770 CPU sec, 1.827 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282525, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (348.00, 163.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 80, #reads = 1084480 mem = 536877712 peak = 1082691584
[M::process] read 678542 sequences (100000086 bp) in 1.695 CPU sec, 1.782 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282621, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 331, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (348.52, 162.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678550 sequences (100000147 bp) in 1.270 CPU sec, 1.264 real sec...
[M::mem_process_seqs] Processed 678976 reads in 126.252 CPU sec, 4.022 real sec
[M::mem_process_seqs] Processed 678690 reads in 79.421 CPU sec, 2.513 real sec
sorting block 81, #reads = 1083473 mem = 536877622 peak = 1082691584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283123, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (349.03, 161.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678832 sequences (100000076 bp) in 2.112 CPU sec, 2.224 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282737, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (348.55, 162.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678880 sequences (100000297 bp) in 1.310 CPU sec, 1.313 real sec...
[M::mem_process_seqs] Processed 678542 reads in 124.952 CPU sec, 3.966 real sec
[M::mem_process_seqs] Processed 678550 reads in 79.360 CPU sec, 2.507 real sec
sorting block 82, #reads = 1084217 mem = 536878024 peak = 1082691584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282576, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 328, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (346.45, 162.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677906 sequences (100000161 bp) in 1.723 CPU sec, 1.800 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282755, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 677832 sequences (100000183 bp) in 1.310 CPU sec, 1.307 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (216, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (346.19, 162.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678832 reads in 126.032 CPU sec, 4.011 real sec
[M::mem_process_seqs] Processed 678880 reads in 78.011 CPU sec, 2.465 real sec
sorting block 83, #reads = 1084283 mem = 536879051 peak = 1082691584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282272, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 335, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (352.04, 162.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677840 sequences (100000098 bp) in 2.065 CPU sec, 2.156 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282023, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 335, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (352.03, 162.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677906 sequences (100000063 bp) in 1.319 CPU sec, 1.316 real sec...
[M::mem_process_seqs] Processed 677906 reads in 125.246 CPU sec, 3.973 real sec
sorting block 84, #reads = 1083263 mem = 536881857 peak = 1082691584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282635, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 336, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (352.55, 163.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677816 sequences (100000256 bp) in 1.381 CPU sec, 1.388 real sec...
[M::mem_process_seqs] Processed 677840 reads in 69.046 CPU sec, 2.190 real sec
sorting block 85, #reads = 1084087 mem = 536876787 peak = 1082691584
[M::mem_process_seqs] Processed 677832 reads in 163.342 CPU sec, 5.180 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282118, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 335, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (352.35, 163.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1186)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677570 sequences (100000124 bp) in 2.097 CPU sec, 2.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282061, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (350.61, 161.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677906 reads in 137.369 CPU sec, 4.351 real sec
[M::process] read 677870 sequences (100000179 bp) in 1.300 CPU sec, 1.301 real sec...
[M::mem_process_seqs] Processed 677816 reads in 79.703 CPU sec, 2.513 real sec
sorting block 86, #reads = 1082972 mem = 536878105 peak = 1082691584
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282048, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (349.20, 160.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678016 sequences (100000031 bp) in 2.053 CPU sec, 2.175 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282129, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (348.73, 160.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677796 sequences (100000229 bp) in 1.280 CPU sec, 1.281 real sec...
[M::mem_process_seqs] Processed 677570 reads in 126.272 CPU sec, 4.006 real sec
sorting block 87, #reads = 1083180 mem = 536879388 peak = 1082691584
[M::mem_process_seqs] Processed 677870 reads in 78.927 CPU sec, 2.494 real sec
sorting block 88, #reads = 1082087 mem = 536880936 peak = 1112588288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282097, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (349.05, 161.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677680 sequences (100000068 bp) in 2.069 CPU sec, 2.167 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281967, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (350.27, 161.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677546 sequences (100000185 bp) in 1.320 CPU sec, 1.318 real sec...
[M::mem_process_seqs] Processed 678016 reads in 125.075 CPU sec, 3.966 real sec
[M::mem_process_seqs] Processed 677796 reads in 78.787 CPU sec, 2.492 real sec
sorting block 89, #reads = 1082703 mem = 536878371 peak = 1112588288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281948, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (350.32, 161.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677674 sequences (100000035 bp) in 2.001 CPU sec, 2.084 real sec...
[M::process] read 677634 sequences (100000079 bp) in 1.300 CPU sec, 1.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282215, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (348.46, 160.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677680 reads in 125.955 CPU sec, 3.996 real sec
[M::mem_process_seqs] Processed 677546 reads in 78.632 CPU sec, 2.489 real sec
sorting block 90, #reads = 1082672 mem = 536877591 peak = 1112588288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282074, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (347.15, 159.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677548 sequences (100000059 bp) in 2.033 CPU sec, 2.113 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282592, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (348.67, 160.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677636 sequences (100000178 bp) in 1.340 CPU sec, 1.334 real sec...
[M::mem_process_seqs] Processed 677674 reads in 125.165 CPU sec, 3.972 real sec
[M::mem_process_seqs] Processed 677634 reads in 78.329 CPU sec, 2.474 real sec
sorting block 91, #reads = 1083035 mem = 536881940 peak = 1112588288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282886, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (349.00, 160.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 92, #reads = 1082482 mem = 536878797 peak = 1112588288
[M::process] read 677286 sequences (100000011 bp) in 1.882 CPU sec, 1.964 real sec...
[M::process] read 677246 sequences (100000220 bp) in 1.290 CPU sec, 1.285 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282480, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 329, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (346.25, 158.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677548 reads in 125.624 CPU sec, 3.989 real sec
[M::mem_process_seqs] Processed 677636 reads in 78.565 CPU sec, 2.485 real sec
sorting block 93, #reads = 1083228 mem = 536877766 peak = 1112588288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282240, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (349.90, 159.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677366 sequences (100000181 bp) in 1.888 CPU sec, 1.965 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282230, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 332, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (349.13, 159.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677310 sequences (100000238 bp) in 1.300 CPU sec, 1.300 real sec...
[M::mem_process_seqs] Processed 677286 reads in 125.589 CPU sec, 3.993 real sec
[M::mem_process_seqs] Processed 677246 reads in 77.714 CPU sec, 2.457 real sec
sorting block 94, #reads = 1081849 mem = 536879172 peak = 1112588288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282340, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 332, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (349.10, 160.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677312 sequences (100000172 bp) in 1.895 CPU sec, 1.965 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282014, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 330, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (346.57, 158.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676908 sequences (100000211 bp) in 1.280 CPU sec, 1.282 real sec...
[M::mem_process_seqs] Processed 677366 reads in 123.162 CPU sec, 3.910 real sec
[M::mem_process_seqs] Processed 677310 reads in 76.744 CPU sec, 2.422 real sec
sorting block 95, #reads = 1082872 mem = 536879460 peak = 1112588288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281942, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (347.19, 157.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677018 sequences (100000119 bp) in 2.048 CPU sec, 2.120 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282562, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 334, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (349.87, 158.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676794 sequences (100000275 bp) in 1.300 CPU sec, 1.302 real sec...
[M::mem_process_seqs] Processed 677312 reads in 120.349 CPU sec, 3.816 real sec
sorting block 96, #reads = 1082347 mem = 536888251 peak = 1112588288
[M::mem_process_seqs] Processed 676908 reads in 75.188 CPU sec, 2.375 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282051, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (352.51, 160.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 97, #reads = 1083529 mem = 536880928 peak = 1112588288
[M::process] read 676630 sequences (100000014 bp) in 2.078 CPU sec, 2.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282031, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 339, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (354.70, 161.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676584 sequences (100000158 bp) in 1.290 CPU sec, 1.282 real sec...
[M::mem_process_seqs] Processed 677018 reads in 120.298 CPU sec, 3.815 real sec
[M::mem_process_seqs] Processed 676794 reads in 76.076 CPU sec, 2.399 real sec
sorting block 98, #reads = 1081166 mem = 536879618 peak = 1112588288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282002, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 340, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (356.87, 163.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682074 sequences (100000112 bp) in 2.370 CPU sec, 2.474 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282152, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 342, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (358.92, 165.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676630 reads in 119.249 CPU sec, 3.780 real sec
[M::mem_process_seqs] Processed 676584 reads in 75.464 CPU sec, 2.373 real sec
[M::process] read 683090 sequences (100000198 bp) in 1.785 CPU sec, 1.825 real sec...
sorting block 99, #reads = 1082009 mem = 536877783 peak = 1112588288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284098, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 307, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (331.11, 161.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682400 sequences (100000059 bp) in 2.313 CPU sec, 2.432 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284530, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 301, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (328.53, 163.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682074 reads in 129.414 CPU sec, 4.115 real sec
[M::mem_process_seqs] Processed 683090 reads in 80.867 CPU sec, 2.543 real sec
[M::process] read 681892 sequences (100000286 bp) in 1.802 CPU sec, 1.854 real sec...
sorting block 100, #reads = 1081360 mem = 536879756 peak = 1112588288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284007, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 306, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (331.69, 163.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681378 sequences (100000079 bp) in 1.974 CPU sec, 2.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284236, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 311, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (334.95, 164.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682400 reads in 128.066 CPU sec, 4.066 real sec
[M::process] read 680854 sequences (100000155 bp) in 1.310 CPU sec, 1.306 real sec...
sorting block 101, #reads = 1085555 mem = 536878167 peak = 1112588288
[M::mem_process_seqs] Processed 681892 reads in 80.077 CPU sec, 2.524 real sec
sorting block 102, #reads = 1084512 mem = 536880307 peak = 1112588288
[M::process] read 680602 sequences (100000130 bp) in 2.084 CPU sec, 2.164 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283923, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 316, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (337.53, 162.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680056 sequences (100000260 bp) in 1.350 CPU sec, 1.353 real sec...
[M::mem_process_seqs] Processed 680854 reads in 68.841 CPU sec, 2.170 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283844, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 313, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (336.06, 162.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283822, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 319, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (340.82, 164.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681378 reads in 196.486 CPU sec, 6.223 real sec
[M::process] read 679932 sequences (100000050 bp) in 1.784 CPU sec, 1.790 real sec...
[M::mem_process_seqs] Processed 680602 reads in 91.417 CPU sec, 2.903 real sec
sorting block 103, #reads = 1083296 mem = 536879725 peak = 1112588288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283728, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 322, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (343.54, 164.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679620 sequences (100000108 bp) in 2.150 CPU sec, 2.238 real sec...
[M::process] read 679034 sequences (100000075 bp) in 1.280 CPU sec, 1.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283618, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 325, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (345.34, 165.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680056 reads in 130.898 CPU sec, 4.155 real sec
sorting block 104, #reads = 1082936 mem = 536884982 peak = 1112588288
[M::mem_process_seqs] Processed 679932 reads in 84.176 CPU sec, 2.664 real sec
sorting block 105, #reads = 1081851 mem = 536882074 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283332, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 327, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (347.23, 166.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678914 sequences (100000228 bp) in 1.883 CPU sec, 1.986 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282639, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 331, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (350.26, 166.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1211)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678748 sequences (100000090 bp) in 1.350 CPU sec, 1.349 real sec...
[M::mem_process_seqs] Processed 679620 reads in 125.477 CPU sec, 3.984 real sec
[M::mem_process_seqs] Processed 679034 reads in 78.855 CPU sec, 2.493 real sec
sorting block 106, #reads = 1082803 mem = 536878549 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283410, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 332, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (350.28, 165.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678896 sequences (100000208 bp) in 2.020 CPU sec, 2.099 real sec...
[M::process] read 678504 sequences (100000286 bp) in 1.320 CPU sec, 1.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283099, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 334, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (352.09, 165.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678914 reads in 126.175 CPU sec, 4.000 real sec
[M::mem_process_seqs] Processed 678748 reads in 79.532 CPU sec, 2.513 real sec
sorting block 107, #reads = 1082690 mem = 536878707 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283665, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 334, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (351.13, 165.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678256 sequences (100000149 bp) in 1.796 CPU sec, 1.895 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283622, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 336, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (353.97, 166.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677790 sequences (100000007 bp) in 1.350 CPU sec, 1.347 real sec...
[M::mem_process_seqs] Processed 678896 reads in 128.856 CPU sec, 4.098 real sec
[M::mem_process_seqs] Processed 678504 reads in 81.063 CPU sec, 2.564 real sec
sorting block 108, #reads = 1082248 mem = 536877939 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282599, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 337, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (354.28, 165.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 109, #reads = 1081593 mem = 536878865 peak = 1133682688
[M::process] read 677848 sequences (100000233 bp) in 1.945 CPU sec, 2.037 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282170, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (355.22, 165.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678260 sequences (100000280 bp) in 1.380 CPU sec, 1.381 real sec...
[M::mem_process_seqs] Processed 678256 reads in 130.508 CPU sec, 4.145 real sec
[M::mem_process_seqs] Processed 677790 reads in 81.836 CPU sec, 2.584 real sec
sorting block 110, #reads = 1080645 mem = 536878437 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282377, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 336, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (353.42, 164.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677962 sequences (100000158 bp) in 2.165 CPU sec, 2.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282935, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (351.01, 163.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677848 reads in 130.575 CPU sec, 4.148 real sec
[M::process] read 677858 sequences (100000239 bp) in 1.632 CPU sec, 1.647 real sec...
[M::mem_process_seqs] Processed 678260 reads in 82.653 CPU sec, 2.598 real sec
sorting block 111, #reads = 1080788 mem = 536879676 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282164, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (352.67, 164.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677808 sequences (100000157 bp) in 2.188 CPU sec, 2.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282192, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (352.31, 164.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678038 sequences (100000226 bp) in 1.340 CPU sec, 1.343 real sec...
[M::mem_process_seqs] Processed 677962 reads in 130.497 CPU sec, 4.143 real sec
[M::mem_process_seqs] Processed 677858 reads in 81.889 CPU sec, 2.585 real sec
sorting block 112, #reads = 1080597 mem = 536877876 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283087, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (352.01, 164.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678026 sequences (100000101 bp) in 1.938 CPU sec, 2.034 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283013, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (351.17, 163.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678006 sequences (100000033 bp) in 1.340 CPU sec, 1.339 real sec...
[M::mem_process_seqs] Processed 677808 reads in 129.743 CPU sec, 4.125 real sec
sorting block 113, #reads = 1080196 mem = 536880878 peak = 1133682688
[M::mem_process_seqs] Processed 678038 reads in 81.659 CPU sec, 2.579 real sec
sorting block 114, #reads = 1080678 mem = 536880792 peak = 1133682688
[M::process] read 677896 sequences (100000059 bp) in 2.055 CPU sec, 2.124 real sec...
[M::process] read 677792 sequences (100000164 bp) in 1.350 CPU sec, 1.353 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282318, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (349.06, 162.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678006 reads in 71.892 CPU sec, 2.266 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282493, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (350.16, 162.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283506, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 336, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (352.54, 162.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677850 sequences (100000269 bp) in 1.631 CPU sec, 1.633 real sec...
[M::mem_process_seqs] Processed 678026 reads in 202.978 CPU sec, 6.428 real sec
sorting block 115, #reads = 1080037 mem = 536879324 peak = 1133682688
[M::mem_process_seqs] Processed 677896 reads in 95.099 CPU sec, 3.029 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282838, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (349.53, 160.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677922 sequences (100000130 bp) in 2.009 CPU sec, 2.125 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282839, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (348.74, 161.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677882 sequences (100000043 bp) in 1.330 CPU sec, 1.323 real sec...
[M::mem_process_seqs] Processed 677792 reads in 131.020 CPU sec, 4.163 real sec
sorting block 116, #reads = 1079045 mem = 536877901 peak = 1133682688
[M::mem_process_seqs] Processed 677850 reads in 82.932 CPU sec, 2.620 real sec
sorting block 117, #reads = 1079909 mem = 536880861 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283004, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (347.64, 160.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677952 sequences (100000243 bp) in 2.090 CPU sec, 2.190 real sec...
[M::process] read 677626 sequences (100000119 bp) in 1.360 CPU sec, 1.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282686, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (344.97, 158.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677922 reads in 129.607 CPU sec, 4.108 real sec
[M::mem_process_seqs] Processed 677882 reads in 80.806 CPU sec, 2.555 real sec
sorting block 118, #reads = 1080271 mem = 536878688 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283029, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (346.42, 159.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677896 sequences (100000021 bp) in 1.810 CPU sec, 1.896 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282488, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (348.50, 160.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677828 sequences (100000042 bp) in 1.330 CPU sec, 1.327 real sec...
[M::mem_process_seqs] Processed 677952 reads in 129.554 CPU sec, 4.121 real sec
[M::mem_process_seqs] Processed 677626 reads in 81.629 CPU sec, 2.580 real sec
sorting block 119, #reads = 1080716 mem = 536876786 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282402, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (349.13, 161.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678022 sequences (100000104 bp) in 2.092 CPU sec, 2.197 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 282991, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (349.05, 162.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677876 sequences (100000155 bp) in 1.360 CPU sec, 1.357 real sec...
[M::mem_process_seqs] Processed 677896 reads in 128.951 CPU sec, 4.089 real sec
sorting block 120, #reads = 1080078 mem = 536881423 peak = 1133682688
[M::mem_process_seqs] Processed 677828 reads in 80.950 CPU sec, 2.558 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282189, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (350.78, 164.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 121, #reads = 1079695 mem = 536879976 peak = 1133682688
[M::process] read 684110 sequences (100000221 bp) in 1.825 CPU sec, 1.896 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282463, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 335, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (353.96, 167.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684224 sequences (100000224 bp) in 1.390 CPU sec, 1.392 real sec...
[M::mem_process_seqs] Processed 678022 reads in 130.140 CPU sec, 4.135 real sec
[M::mem_process_seqs] Processed 677876 reads in 82.532 CPU sec, 2.608 real sec
sorting block 122, #reads = 1078569 mem = 536879574 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285773, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 294, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (321.28, 159.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683104 sequences (100000206 bp) in 2.128 CPU sec, 2.207 real sec...
[M::process] read 682418 sequences (100000134 bp) in 1.380 CPU sec, 1.373 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285519, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 292, 423)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 883)
[M::mem_pestat] mean and std.dev: (318.44, 156.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684110 reads in 136.440 CPU sec, 4.329 real sec
[M::mem_process_seqs] Processed 684224 reads in 85.026 CPU sec, 2.695 real sec
sorting block 123, #reads = 1079857 mem = 536877424 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285243, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 300, 429)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (323.56, 156.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682030 sequences (100000266 bp) in 1.823 CPU sec, 1.888 real sec...
[M::process] read 681566 sequences (100000194 bp) in 1.360 CPU sec, 1.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284514, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 304, 432)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (326.23, 156.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683104 reads in 133.137 CPU sec, 4.231 real sec
[M::mem_process_seqs] Processed 682418 reads in 83.529 CPU sec, 2.641 real sec
sorting block 124, #reads = 1081731 mem = 536878757 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284753, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 308, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (329.83, 158.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681832 sequences (100000182 bp) in 1.965 CPU sec, 2.015 real sec...
[M::process] read 681910 sequences (100000031 bp) in 1.380 CPU sec, 1.381 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284393, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 310, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (332.06, 159.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1155)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682030 reads in 131.327 CPU sec, 4.166 real sec
sorting block 125, #reads = 1080984 mem = 536879603 peak = 1133682688
[M::mem_process_seqs] Processed 681566 reads in 82.037 CPU sec, 2.599 real sec
sorting block 126, #reads = 1081639 mem = 536882634 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285232, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 309, 440)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (330.42, 157.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681474 sequences (100000248 bp) in 1.944 CPU sec, 2.014 real sec...
[M::process] read 680744 sequences (100000270 bp) in 1.370 CPU sec, 1.368 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284663, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 307, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (329.10, 157.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681832 reads in 134.337 CPU sec, 4.267 real sec
[M::mem_process_seqs] Processed 681910 reads in 84.372 CPU sec, 2.672 real sec
sorting block 127, #reads = 1080217 mem = 536882767 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284690, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 312, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (334.04, 160.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680292 sequences (100000220 bp) in 2.101 CPU sec, 2.174 real sec...
[M::process] read 680238 sequences (100000040 bp) in 1.360 CPU sec, 1.362 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283854, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 317, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (337.72, 161.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681474 reads in 135.385 CPU sec, 4.297 real sec
[M::mem_process_seqs] Processed 680744 reads in 82.424 CPU sec, 2.611 real sec
sorting block 128, #reads = 1079029 mem = 536878436 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284266, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 320, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (338.93, 160.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679822 sequences (100000132 bp) in 1.862 CPU sec, 1.923 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283984, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 322, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (340.82, 161.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679886 sequences (100000179 bp) in 1.360 CPU sec, 1.357 real sec...
[M::mem_process_seqs] Processed 680292 reads in 129.063 CPU sec, 4.105 real sec
[M::mem_process_seqs] Processed 680238 reads in 81.408 CPU sec, 2.571 real sec
sorting block 129, #reads = 1080998 mem = 536878189 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283607, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (342.99, 161.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679310 sequences (100000246 bp) in 2.192 CPU sec, 2.273 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284379, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 326, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (344.02, 162.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678924 sequences (100000216 bp) in 1.370 CPU sec, 1.372 real sec...
[M::mem_process_seqs] Processed 679822 reads in 129.308 CPU sec, 4.101 real sec
sorting block 130, #reads = 1081299 mem = 536879694 peak = 1133682688
[M::mem_process_seqs] Processed 679886 reads in 81.393 CPU sec, 2.571 real sec
sorting block 131, #reads = 1080728 mem = 536878779 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283977, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 328, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (347.55, 165.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1198)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678598 sequences (100000251 bp) in 1.894 CPU sec, 1.968 real sec...
[M::process] read 678294 sequences (100000234 bp) in 1.350 CPU sec, 1.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283580, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 331, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (349.37, 164.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679310 reads in 131.460 CPU sec, 4.177 real sec
[M::mem_process_seqs] Processed 678924 reads in 83.632 CPU sec, 2.644 real sec
sorting block 132, #reads = 1079577 mem = 536878659 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283316, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 333, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (351.39, 165.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678372 sequences (100000248 bp) in 2.016 CPU sec, 2.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283261, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 678216 sequences (100000038 bp) in 1.340 CPU sec, 1.342 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (218, 334, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (352.09, 165.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678598 reads in 131.155 CPU sec, 4.163 real sec
[M::mem_process_seqs] Processed 678294 reads in 82.523 CPU sec, 2.610 real sec
sorting block 133, #reads = 1079024 mem = 536878822 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283149, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 333, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (351.54, 165.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678006 sequences (100000171 bp) in 1.834 CPU sec, 1.893 real sec...
[M::process] read 678168 sequences (100000177 bp) in 1.330 CPU sec, 1.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283149, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (351.10, 164.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678372 reads in 131.408 CPU sec, 4.176 real sec
sorting block 134, #reads = 1079276 mem = 536879625 peak = 1133682688
[M::mem_process_seqs] Processed 678216 reads in 82.701 CPU sec, 2.614 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283072, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 333, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (352.13, 166.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677984 sequences (100000231 bp) in 2.073 CPU sec, 2.134 real sec...
[M::process] read 677728 sequences (100000019 bp) in 1.330 CPU sec, 1.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283097, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (353.51, 165.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678006 reads in 131.482 CPU sec, 4.171 real sec
sorting block 135, #reads = 1079073 mem = 536882254 peak = 1133682688
[M::mem_process_seqs] Processed 678168 reads in 82.176 CPU sec, 2.599 real sec
sorting block 136, #reads = 1080076 mem = 536877626 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282800, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (352.94, 165.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677792 sequences (100000189 bp) in 1.889 CPU sec, 1.960 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282792, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 335, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (353.45, 165.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678070 sequences (100000068 bp) in 1.330 CPU sec, 1.330 real sec...
[M::mem_process_seqs] Processed 677984 reads in 130.635 CPU sec, 4.154 real sec
[M::mem_process_seqs] Processed 677728 reads in 81.666 CPU sec, 2.584 real sec
sorting block 137, #reads = 1079987 mem = 536881102 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282940, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 337, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (354.29, 165.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677632 sequences (100000114 bp) in 2.129 CPU sec, 2.192 real sec...
[M::process] read 677938 sequences (100000161 bp) in 1.330 CPU sec, 1.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282816, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 335, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972)
[M::mem_pestat] mean and std.dev: (355.18, 168.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677792 reads in 129.399 CPU sec, 4.103 real sec
[M::mem_process_seqs] Processed 678070 reads in 81.191 CPU sec, 2.567 real sec
sorting block 138, #reads = 1080709 mem = 536878195 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282581, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 337, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (355.77, 167.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1213)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677362 sequences (100000169 bp) in 1.899 CPU sec, 1.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283138, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 338, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (356.90, 169.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677386 sequences (100000196 bp) in 1.320 CPU sec, 1.318 real sec...
[M::mem_process_seqs] Processed 677632 reads in 126.868 CPU sec, 4.034 real sec
[M::mem_process_seqs] Processed 677938 reads in 79.728 CPU sec, 2.518 real sec
sorting block 139, #reads = 1080438 mem = 536880739 peak = 1133682688
[M::process] read 677498 sequences (100000092 bp) in 2.094 CPU sec, 2.154 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282254, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 340, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (360.53, 171.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677614 sequences (100000216 bp) in 1.340 CPU sec, 1.342 real sec...
[M::mem_process_seqs] Processed 677386 reads in 74.398 CPU sec, 2.349 real sec
sorting block 140, #reads = 1080498 mem = 536881170 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282106, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 341, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982)
[M::mem_pestat] mean and std.dev: (361.19, 171.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1235)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282508, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 342, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (362.42, 173.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678226 sequences (100000152 bp) in 1.533 CPU sec, 1.539 real sec...
[M::mem_process_seqs] Processed 677362 reads in 199.946 CPU sec, 6.344 real sec
[M::mem_process_seqs] Processed 677498 reads in 95.392 CPU sec, 3.039 real sec
sorting block 141, #reads = 1079679 mem = 536878970 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282662, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 340, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (362.69, 175.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682414 sequences (100000162 bp) in 2.004 CPU sec, 2.079 real sec...
[M::process] read 682578 sequences (100000072 bp) in 1.300 CPU sec, 1.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282477, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 339, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002)
[M::mem_pestat] mean and std.dev: (360.65, 175.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677614 reads in 140.721 CPU sec, 4.480 real sec
sorting block 142, #reads = 1079259 mem = 536882395 peak = 1133682688
[M::mem_process_seqs] Processed 678226 reads in 93.356 CPU sec, 2.957 real sec
sorting block 143, #reads = 1074834 mem = 536878891 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284494, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 305, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (330.24, 162.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681854 sequences (100000067 bp) in 2.165 CPU sec, 2.281 real sec...
[M::process] read 681038 sequences (100000123 bp) in 1.340 CPU sec, 1.338 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284431, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 304, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (327.78, 159.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682414 reads in 143.589 CPU sec, 4.562 real sec
[M::mem_process_seqs] Processed 682578 reads in 85.329 CPU sec, 2.701 real sec
sorting block 144, #reads = 1075675 mem = 536879691 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284148, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 309, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (330.66, 159.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681018 sequences (100000178 bp) in 1.763 CPU sec, 1.827 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283786, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 312, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (332.30, 157.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680758 sequences (100000057 bp) in 1.330 CPU sec, 1.328 real sec...
[M::mem_process_seqs] Processed 681854 reads in 131.619 CPU sec, 4.197 real sec
[M::mem_process_seqs] Processed 681038 reads in 82.115 CPU sec, 2.596 real sec
sorting block 145, #reads = 1084640 mem = 536881071 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284054, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 314, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (333.94, 158.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680950 sequences (100000054 bp) in 2.033 CPU sec, 2.106 real sec...
[M::process] read 680338 sequences (100000135 bp) in 1.360 CPU sec, 1.359 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284211, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 316, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (335.48, 158.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681018 reads in 132.361 CPU sec, 4.204 real sec
sorting block 146, #reads = 1083448 mem = 536879751 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284050, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 315, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::process] read 680084 sequences (100000058 bp) in 1.434 CPU sec, 1.440 real sec...
[M::mem_pestat] mean and std.dev: (334.92, 158.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680950 reads in 72.797 CPU sec, 2.310 real sec
[M::mem_process_seqs] Processed 680758 reads in 173.169 CPU sec, 5.504 real sec
sorting block 147, #reads = 1081632 mem = 536879490 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283693, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 317, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (336.61, 158.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679662 sequences (100000179 bp) in 2.210 CPU sec, 2.333 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283184, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 322, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (341.43, 161.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680338 reads in 144.605 CPU sec, 4.588 real sec
[M::process] read 679840 sequences (100000079 bp) in 1.300 CPU sec, 1.301 real sec...
[M::mem_process_seqs] Processed 680084 reads in 83.064 CPU sec, 2.625 real sec
sorting block 148, #reads = 1082818 mem = 536882698 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283652, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 324, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (342.54, 161.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1176)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679572 sequences (100000126 bp) in 2.053 CPU sec, 2.146 real sec...
[M::process] read 679540 sequences (100000172 bp) in 1.280 CPU sec, 1.283 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283237, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 325, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (344.27, 163.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679662 reads in 131.065 CPU sec, 4.161 real sec
sorting block 149, #reads = 1082274 mem = 536879045 peak = 1133682688
[M::mem_process_seqs] Processed 679840 reads in 81.538 CPU sec, 2.588 real sec
sorting block 150, #reads = 1083466 mem = 536877218 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283298, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 328, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (345.95, 163.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679662 sequences (100000228 bp) in 2.000 CPU sec, 2.068 real sec...
[M::process] read 679300 sequences (100000171 bp) in 1.330 CPU sec, 1.329 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282768, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (343.98, 161.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679572 reads in 138.498 CPU sec, 4.407 real sec
[M::mem_process_seqs] Processed 679540 reads in 85.771 CPU sec, 2.717 real sec
sorting block 151, #reads = 1081607 mem = 536883662 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283217, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 326, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (343.81, 161.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679148 sequences (100000102 bp) in 2.129 CPU sec, 2.226 real sec...
[M::process] read 678596 sequences (100000285 bp) in 1.300 CPU sec, 1.306 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283163, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 327, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (345.11, 162.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1189)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679662 reads in 133.838 CPU sec, 4.252 real sec
[M::mem_process_seqs] Processed 679300 reads in 83.226 CPU sec, 2.640 real sec
sorting block 152, #reads = 1079474 mem = 536879343 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282953, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 330, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (347.46, 163.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678860 sequences (100000034 bp) in 2.207 CPU sec, 2.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282783, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 332, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (349.48, 163.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678262 sequences (100000190 bp) in 1.330 CPU sec, 1.328 real sec...
[M::mem_process_seqs] Processed 679148 reads in 133.393 CPU sec, 4.236 real sec
[M::mem_process_seqs] Processed 678596 reads in 83.837 CPU sec, 2.655 real sec
sorting block 153, #reads = 1080667 mem = 536879072 peak = 1133682688
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283098, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 331, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (348.00, 162.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677814 sequences (100000103 bp) in 2.220 CPU sec, 2.340 real sec...
[M::process] read 678144 sequences (100000086 bp) in 1.300 CPU sec, 1.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282773, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 335, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (351.74, 163.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678860 reads in 135.486 CPU sec, 4.302 real sec
sorting block 154, #reads = 1080859 mem = 536880525 peak = 1133682688
[M::mem_process_seqs] Processed 678262 reads in 87.940 CPU sec, 2.788 real sec
sorting block 155, #reads = 1080325 mem = 536882606 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283156, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 336, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953)
[M::mem_pestat] mean and std.dev: (352.52, 162.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1197)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678052 sequences (100000187 bp) in 2.121 CPU sec, 2.204 real sec...
[M::process] read 677886 sequences (100000138 bp) in 1.410 CPU sec, 1.406 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282819, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (351.10, 163.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677814 reads in 135.138 CPU sec, 4.289 real sec
[M::mem_process_seqs] Processed 678144 reads in 84.965 CPU sec, 2.691 real sec
sorting block 156, #reads = 1080719 mem = 536881482 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282849, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 334, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (351.76, 163.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677966 sequences (100000038 bp) in 2.219 CPU sec, 2.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282732, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 335, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (353.21, 164.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1201)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677894 sequences (100000122 bp) in 1.310 CPU sec, 1.314 real sec...
[M::mem_process_seqs] Processed 678052 reads in 134.970 CPU sec, 4.287 real sec
[M::mem_process_seqs] Processed 677886 reads in 84.538 CPU sec, 2.679 real sec
sorting block 157, #reads = 1080590 mem = 536879293 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283222, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 333, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (350.17, 162.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1187)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678018 sequences (100000053 bp) in 2.162 CPU sec, 2.239 real sec...
[M::process] read 678050 sequences (100000292 bp) in 1.330 CPU sec, 1.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282795, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (351.13, 163.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677966 reads in 135.679 CPU sec, 4.309 real sec
[M::mem_process_seqs] Processed 677894 reads in 85.354 CPU sec, 2.704 real sec
sorting block 158, #reads = 1079866 mem = 536877166 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282962, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (351.63, 165.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677922 sequences (100000023 bp) in 2.124 CPU sec, 2.198 real sec...
[M::process] read 677982 sequences (100000074 bp) in 1.300 CPU sec, 1.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283307, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (349.17, 162.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678018 reads in 137.645 CPU sec, 4.372 real sec
sorting block 159, #reads = 1079187 mem = 536878646 peak = 1157971968
[M::mem_process_seqs] Processed 678050 reads in 88.158 CPU sec, 2.797 real sec
sorting block 160, #reads = 1079907 mem = 536878375 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282757, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 332, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (350.81, 163.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677774 sequences (100000206 bp) in 2.094 CPU sec, 2.147 real sec...
[M::process] read 677806 sequences (100000119 bp) in 1.330 CPU sec, 1.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283010, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (351.24, 164.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677922 reads in 138.020 CPU sec, 4.383 real sec
[M::mem_process_seqs] Processed 677982 reads in 85.470 CPU sec, 2.713 real sec
sorting block 161, #reads = 1077126 mem = 536877542 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282868, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (352.26, 164.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677934 sequences (100000296 bp) in 2.099 CPU sec, 2.169 real sec...
[M::process] read 678056 sequences (100000258 bp) in 1.330 CPU sec, 1.322 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282954, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (353.00, 165.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1207)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677774 reads in 135.949 CPU sec, 4.320 real sec
[M::mem_process_seqs] Processed 677806 reads in 85.631 CPU sec, 2.713 real sec
sorting block 162, #reads = 1078562 mem = 536879526 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282846, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962)
[M::mem_pestat] mean and std.dev: (352.38, 166.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678398 sequences (100000223 bp) in 2.166 CPU sec, 2.237 real sec...
[M::process] read 682186 sequences (100000136 bp) in 1.380 CPU sec, 1.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282412, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 334, 469)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969)
[M::mem_pestat] mean and std.dev: (354.58, 168.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677934 reads in 140.392 CPU sec, 4.456 real sec
sorting block 163, #reads = 1077866 mem = 536879145 peak = 1157971968
[M::mem_process_seqs] Processed 678056 reads in 89.649 CPU sec, 2.843 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283149, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 333, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970)
[M::mem_pestat] mean and std.dev: (353.80, 169.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1221)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685740 sequences (100000188 bp) in 2.153 CPU sec, 2.209 real sec...
[M::process] read 684272 sequences (100000287 bp) in 1.330 CPU sec, 1.332 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284288, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 305, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (331.06, 162.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1164)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678398 reads in 153.448 CPU sec, 4.880 real sec
sorting block 164, #reads = 1076105 mem = 536879993 peak = 1157971968
[M::mem_process_seqs] Processed 682186 reads in 99.462 CPU sec, 3.161 real sec
sorting block 165, #reads = 1068092 mem = 536880517 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286095, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 283, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (309.15, 151.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1080)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683346 sequences (100000185 bp) in 2.207 CPU sec, 2.271 real sec...
[M::process] read 682990 sequences (100000208 bp) in 1.350 CPU sec, 1.355 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285630, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 292, 421)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (316.63, 153.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685740 reads in 139.696 CPU sec, 4.435 real sec
[M::mem_process_seqs] Processed 684272 reads in 86.997 CPU sec, 2.762 real sec
sorting block 166, #reads = 1081405 mem = 536881023 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284850, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 296, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (320.04, 154.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1115)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 682212 sequences (100000179 bp) in 2.117 CPU sec, 2.176 real sec...
[M::process] read 683182 sequences (100000257 bp) in 1.340 CPU sec, 1.341 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284426, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 300, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (322.44, 154.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683346 reads in 138.006 CPU sec, 4.383 real sec
[M::mem_process_seqs] Processed 682990 reads in 86.404 CPU sec, 2.745 real sec
sorting block 167, #reads = 1080882 mem = 536877203 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284716, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 305, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (327.23, 156.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684080 sequences (100000274 bp) in 2.137 CPU sec, 2.198 real sec...
[M::process] read 682500 sequences (100000087 bp) in 1.370 CPU sec, 1.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284930, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 297, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (321.38, 155.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 682212 reads in 138.416 CPU sec, 4.394 real sec
sorting block 168, #reads = 1079739 mem = 536878755 peak = 1157971968
[M::mem_process_seqs] Processed 683182 reads in 87.154 CPU sec, 2.766 real sec
sorting block 169, #reads = 1080069 mem = 536879679 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285071, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 293, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (316.28, 151.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681946 sequences (100000184 bp) in 2.124 CPU sec, 2.189 real sec...
[M::process] read 682734 sequences (100000204 bp) in 1.340 CPU sec, 1.338 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284855, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 303, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (325.81, 156.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684080 reads in 145.145 CPU sec, 4.613 real sec
[M::mem_process_seqs] Processed 682500 reads in 89.463 CPU sec, 2.848 real sec
sorting block 170, #reads = 1076542 mem = 536885940 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284323, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 310, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (332.11, 159.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680686 sequences (100000256 bp) in 2.172 CPU sec, 2.243 real sec...
[M::process] read 681078 sequences (100000277 bp) in 1.350 CPU sec, 1.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284836, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 297, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (323.23, 157.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 681946 reads in 138.738 CPU sec, 4.405 real sec
[M::mem_process_seqs] Processed 682734 reads in 86.672 CPU sec, 2.749 real sec
sorting block 171, #reads = 1079175 mem = 536879664 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284469, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 318, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (338.77, 161.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680702 sequences (100000036 bp) in 2.173 CPU sec, 2.241 real sec...
[M::process] read 679834 sequences (100000298 bp) in 1.330 CPU sec, 1.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283803, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 315, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (335.80, 159.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680686 reads in 140.938 CPU sec, 4.479 real sec
sorting block 172, #reads = 1080594 mem = 536879468 peak = 1157971968
[M::process] read 679424 sequences (100000016 bp) in 1.589 CPU sec, 1.592 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283564, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 319, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (339.17, 161.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 680702 reads in 82.804 CPU sec, 2.843 real sec
[M::mem_process_seqs] Processed 681078 reads in 189.027 CPU sec, 6.216 real sec
sorting block 173, #reads = 1077947 mem = 536878866 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283285, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 326, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956)
[M::mem_pestat] mean and std.dev: (345.02, 162.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679078 sequences (100000159 bp) in 2.007 CPU sec, 2.094 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283156, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 327, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (346.09, 163.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678922 sequences (100000111 bp) in 1.340 CPU sec, 1.340 real sec...
[M::mem_process_seqs] Processed 679834 reads in 153.422 CPU sec, 4.869 real sec
[M::mem_process_seqs] Processed 679424 reads in 88.938 CPU sec, 2.813 real sec
sorting block 174, #reads = 1077385 mem = 536880000 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283329, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 327, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955)
[M::mem_pestat] mean and std.dev: (346.37, 162.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678376 sequences (100000058 bp) in 2.059 CPU sec, 2.183 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283557, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (346.30, 161.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678560 sequences (100000176 bp) in 1.330 CPU sec, 1.331 real sec...
[M::mem_process_seqs] Processed 679078 reads in 142.234 CPU sec, 4.530 real sec
sorting block 175, #reads = 1077937 mem = 536879403 peak = 1157971968
[M::mem_process_seqs] Processed 678922 reads in 90.538 CPU sec, 2.867 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 283019, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 330, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (347.90, 161.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 176, #reads = 1077860 mem = 536881728 peak = 1157971968
[M::process] read 678386 sequences (100000159 bp) in 2.191 CPU sec, 2.291 real sec...
[M::process] read 678488 sequences (100000256 bp) in 1.340 CPU sec, 1.342 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282704, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 329, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (348.02, 162.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678376 reads in 140.682 CPU sec, 4.470 real sec
[M::mem_process_seqs] Processed 678560 reads in 87.967 CPU sec, 2.788 real sec
sorting block 177, #reads = 1077780 mem = 536877519 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282816, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 329, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954)
[M::mem_pestat] mean and std.dev: (348.81, 163.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1200)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678250 sequences (100000107 bp) in 1.796 CPU sec, 1.872 real sec...
[M::process] read 678116 sequences (100000230 bp) in 1.330 CPU sec, 1.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283098, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 331, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (350.70, 165.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1209)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678386 reads in 137.120 CPU sec, 4.372 real sec
[M::mem_process_seqs] Processed 678488 reads in 85.876 CPU sec, 2.721 real sec
sorting block 178, #reads = 1079631 mem = 536880537 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282160, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 333, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 965)
[M::mem_pestat] mean and std.dev: (351.85, 165.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677886 sequences (100000165 bp) in 2.110 CPU sec, 2.190 real sec...
[M::process] read 677600 sequences (100000245 bp) in 1.320 CPU sec, 1.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282660, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 333, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959)
[M::mem_pestat] mean and std.dev: (351.12, 163.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678250 reads in 136.774 CPU sec, 4.344 real sec
[M::mem_process_seqs] Processed 678116 reads in 85.214 CPU sec, 2.707 real sec
sorting block 179, #reads = 1079430 mem = 536879779 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282354, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (349.46, 160.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1180)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677490 sequences (100000269 bp) in 1.802 CPU sec, 1.877 real sec...
[M::process] read 677298 sequences (100000200 bp) in 1.310 CPU sec, 1.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282113, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (351.49, 163.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1196)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677886 reads in 134.163 CPU sec, 4.276 real sec
sorting block 180, #reads = 1079300 mem = 536876466 peak = 1157971968
[M::mem_process_seqs] Processed 677600 reads in 83.655 CPU sec, 2.650 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282351, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 336, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957)
[M::mem_pestat] mean and std.dev: (353.82, 163.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1202)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 181, #reads = 1079820 mem = 536886136 peak = 1157971968
[M::process] read 677222 sequences (100000156 bp) in 2.217 CPU sec, 2.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282669, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (352.57, 162.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677352 sequences (100000150 bp) in 1.310 CPU sec, 1.312 real sec...
[M::mem_process_seqs] Processed 677490 reads in 133.268 CPU sec, 4.230 real sec
[M::mem_process_seqs] Processed 677298 reads in 83.331 CPU sec, 2.643 real sec
sorting block 182, #reads = 1080411 mem = 536879079 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282306, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 337, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960)
[M::mem_pestat] mean and std.dev: (354.99, 164.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677406 sequences (100000099 bp) in 1.823 CPU sec, 1.889 real sec...
[M::process] read 677854 sequences (100000229 bp) in 1.320 CPU sec, 1.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282498, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 337, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (355.33, 165.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1203)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677222 reads in 132.924 CPU sec, 4.229 real sec
[M::mem_process_seqs] Processed 677352 reads in 83.455 CPU sec, 2.645 real sec
sorting block 183, #reads = 1079125 mem = 536878295 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282332, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 338, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966)
[M::mem_pestat] mean and std.dev: (356.71, 166.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1214)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677894 sequences (100000213 bp) in 2.246 CPU sec, 2.340 real sec...
[M::process] read 73116 sequences (10756079 bp) in 0.380 CPU sec, 0.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282320, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 338, 470)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 968)
[M::mem_pestat] mean and std.dev: (356.73, 168.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677406 reads in 131.076 CPU sec, 4.189 real sec
[M::mem_process_seqs] Processed 677854 reads in 81.960 CPU sec, 2.623 real sec
sorting block 184, #reads = 1079884 mem = 536878315 peak = 1157971968
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282655, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 333, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (353.35, 166.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1204)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 30431, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 318, 444)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (336.63, 158.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677894 reads in 81.040 CPU sec, 2.636 real sec
[M::mem_process_seqs] Processed 73116 reads in 21.085 CPU sec, 0.686 real sec
sorting block 185, #reads = 1079749 mem = 536879091 peak = 1177632768
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 200970688
overall: 69857075200 mem 22890.980 user 469.975 sys 758.419 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_cVw-T1-TDNA-1_A23MHWWLT4_1.L002\tPU:L002\tSM:659_cVw-T1-TDNA-1_A23MHWWLT4_1\tLB:659_cVw-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_cVw-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_cVw-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 758.459 sec; CPU: 23360.958 sec
sorting block 186, #reads = 602682 mem = 300721489 peak = 1177632768
uniq header count 1
merging 187 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 10541311577
output reads: 201834270
bam_mem_sort: 187 calls 81.794 user 1.462 sys 83.767 real
bam_write: 187 calls 117.585 user 4.148 sys 122.982 real
execute: 1 calls 34.916 user 2.009 sys 817.770 real
merge_files: 1 calls 34.900 user 1.978 sys 58.389 real
parse_chunk: 2985 calls 109.145 user 25.125 sys 136.541 real
read_chunk: 8042 calls 64.712 user 63.412 sys 718.879 real
sort_block: 1 calls 47.598 user 62.887 sys 693.252 real
write_chunk: 5055 calls 34.568 user 1.651 sys 57.461 real
overall: 3279413248 mem 2304.044 user 114.063 sys 817.795 real
ls: cannot access '*.cram': No such file or directory