This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1136_6TL-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1813.341 CPU sec, 66.660 real sec [M::process] read 703956 sequences (100000246 bp) in 1.648 CPU sec, 1.656 real sec... [M::process] read 703862 sequences (100000254 bp) in 1.749 CPU sec, 1.744 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 289822, 20, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 280, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (226.36, 83.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 232, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (256.91, 129.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (595, 797, 1333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2809) [M::mem_pestat] mean and std.dev: (851.42, 548.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 260, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (284.11, 164.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703336 sequences (100000172 bp) in 1.817 CPU sec, 1.819 real sec... [M::process] read 702950 sequences (100000101 bp) in 1.850 CPU sec, 1.850 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (58, 289605, 23, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 266, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (257.72, 118.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 231, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (256.04, 128.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (343, 490, 1337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3325) [M::mem_pestat] mean and std.dev: (741.57, 592.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4319) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 273, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (245.62, 93.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703956 reads in 205.940 CPU sec, 6.485 real sec [M::mem_process_seqs] Processed 703862 reads in 143.418 CPU sec, 4.579 real sec sorting block 0, #reads = 1088729 mem = 536879588 peak = 603119616 [M::process] read 702962 sequences (100000219 bp) in 3.510 CPU sec, 3.819 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 289104, 22, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 245, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (306.92, 238.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 234, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (260.24, 131.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (435, 637, 1453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3489) [M::mem_pestat] mean and std.dev: (901.38, 732.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4507) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 278, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (282.98, 133.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703250 sequences (100000242 bp) in 2.113 CPU sec, 2.116 real sec... [M::mem_process_seqs] Processed 702950 reads in 117.663 CPU sec, 3.730 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 289065, 25, 94) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 263, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124) [M::mem_pestat] mean and std.dev: (325.22, 216.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 233, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (258.08, 130.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 895) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (483, 953, 1627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3915) [M::mem_pestat] mean and std.dev: (933.58, 494.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5059) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 301, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013) [M::mem_pestat] mean and std.dev: (324.27, 200.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1289) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 289553, 20, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 264, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (284.17, 184.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 236, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (262.35, 134.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (595, 2644, 3397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9001) [M::mem_pestat] mean and std.dev: (2049.15, 1319.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11803) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 293, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (307.41, 135.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701062 sequences (100000106 bp) in 2.330 CPU sec, 2.339 real sec... [M::mem_process_seqs] Processed 703336 reads in 323.780 CPU sec, 10.316 real sec sorting block 1, #reads = 1086771 mem = 536878377 peak = 730521600 [M::mem_process_seqs] Processed 702962 reads in 172.670 CPU sec, 5.544 real sec sorting block 2, #reads = 1087613 mem = 536886383 peak = 880054272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (91, 289679, 26, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 281, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (278.38, 133.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 233, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (259.52, 132.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (604, 755, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2119) [M::mem_pestat] mean and std.dev: (594.80, 262.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2624) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 294, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1277) [M::mem_pestat] mean and std.dev: (348.63, 264.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700046 sequences (100000133 bp) in 3.179 CPU sec, 3.346 real sec... [M::process] read 699266 sequences (100000085 bp) in 1.676 CPU sec, 1.693 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (101, 289130, 23, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 250, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (340.13, 224.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 242, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (268.26, 136.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (552, 1091, 2637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6807) [M::mem_pestat] mean and std.dev: (1722.17, 1864.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9181) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 278, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (287.45, 155.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703250 reads in 209.344 CPU sec, 6.692 real sec [M::mem_process_seqs] Processed 701062 reads in 143.451 CPU sec, 4.602 real sec sorting block 3, #reads = 1087945 mem = 536887128 peak = 880054272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 288929, 18, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 329, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (318.03, 139.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (270.92, 137.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (663, 1661, 2793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7053) [M::mem_pestat] mean and std.dev: (2264.39, 1955.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10088) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 239, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1366) [M::mem_pestat] mean and std.dev: (314.48, 226.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698960 sequences (100000121 bp) in 3.473 CPU sec, 3.679 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (108, 288374, 22, 96) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 318, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231) [M::mem_pestat] mean and std.dev: (346.12, 242.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 247, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (272.31, 137.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 859, 1013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2453) [M::mem_pestat] mean and std.dev: (692.67, 424.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 269, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1121) [M::mem_pestat] mean and std.dev: (309.10, 179.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1432) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700048 sequences (100000202 bp) in 2.235 CPU sec, 2.244 real sec... [M::mem_process_seqs] Processed 700046 reads in 205.160 CPU sec, 6.533 real sec [M::mem_process_seqs] Processed 699266 reads in 142.567 CPU sec, 4.540 real sec sorting block 4, #reads = 1087913 mem = 536885507 peak = 880054272 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 287996, 32, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (153, 211, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113) [M::mem_pestat] mean and std.dev: (249.92, 163.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 247, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (272.42, 137.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (572, 1193, 1788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4220) [M::mem_pestat] mean and std.dev: (1077.39, 780.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5436) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 277, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (278.89, 154.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697740 sequences (100000208 bp) in 2.561 CPU sec, 2.743 real sec... [M::process] read 697682 sequences (100000069 bp) in 1.848 CPU sec, 1.856 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 288691, 22, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 286, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1239) [M::mem_pestat] mean and std.dev: (340.39, 206.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (267.35, 134.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (379, 697, 1529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3829) [M::mem_pestat] mean and std.dev: (885.33, 685.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4979) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 244, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2034) [M::mem_pestat] mean and std.dev: (381.90, 401.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2655) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698960 reads in 205.189 CPU sec, 6.565 real sec sorting block 5, #reads = 1086210 mem = 536883415 peak = 880054272 [M::mem_process_seqs] Processed 700048 reads in 145.516 CPU sec, 4.659 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 287692, 30, 90) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 302, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792) [M::mem_pestat] mean and std.dev: (285.10, 103.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (273.52, 136.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (483, 793, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1974) [M::mem_pestat] mean and std.dev: (823.33, 487.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2774) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 313, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1538) [M::mem_pestat] mean and std.dev: (405.19, 306.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1985) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 6, #reads = 1085333 mem = 536880474 peak = 885313536 [M::process] read 696826 sequences (100000266 bp) in 3.332 CPU sec, 3.487 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 287918, 40, 97) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 266, 707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1727) [M::mem_pestat] mean and std.dev: (378.63, 298.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (274.93, 138.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (679, 1044, 2224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5314) [M::mem_pestat] mean and std.dev: (1323.03, 1102.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6859) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 302, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200) [M::mem_pestat] mean and std.dev: (346.38, 217.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1530) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696662 sequences (100000022 bp) in 2.440 CPU sec, 2.436 real sec... [M::mem_process_seqs] Processed 697740 reads in 209.886 CPU sec, 6.686 real sec [M::mem_process_seqs] Processed 697682 reads in 146.691 CPU sec, 4.667 real sec sorting block 7, #reads = 1084667 mem = 536880130 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 287493, 14, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 224, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (286.84, 220.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1282) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (275.58, 137.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (195, 972, 1170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3120) [M::mem_pestat] mean and std.dev: (645.25, 513.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4095) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 284, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1257) [M::mem_pestat] mean and std.dev: (350.76, 247.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697176 sequences (100000108 bp) in 2.883 CPU sec, 3.068 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 287606, 15, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 238, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (268.87, 152.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (275.22, 136.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (188, 206, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025) [M::mem_pestat] mean and std.dev: (245.92, 137.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 360, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1421) [M::mem_pestat] mean and std.dev: (409.81, 326.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697428 sequences (100000262 bp) in 2.019 CPU sec, 2.028 real sec... [M::mem_process_seqs] Processed 696826 reads in 202.442 CPU sec, 6.472 real sec [M::mem_process_seqs] Processed 696662 reads in 140.726 CPU sec, 4.482 real sec sorting block 8, #reads = 1084934 mem = 536880990 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 287788, 12, 99) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 302, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072) [M::mem_pestat] mean and std.dev: (316.56, 203.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1373) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (273.62, 136.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (291, 858, 2867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8019) [M::mem_pestat] mean and std.dev: (791.10, 820.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10595) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 271, 950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2496) [M::mem_pestat] mean and std.dev: (514.17, 591.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697152 sequences (100000030 bp) in 3.346 CPU sec, 3.491 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 287232, 28, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 354, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2123) [M::mem_pestat] mean and std.dev: (490.87, 445.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (271.90, 134.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (461, 689, 1041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2201) [M::mem_pestat] mean and std.dev: (670.52, 367.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2781) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 279, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015) [M::mem_pestat] mean and std.dev: (331.96, 205.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1290) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697176 reads in 212.837 CPU sec, 6.816 real sec [M::process] read 696442 sequences (100000154 bp) in 2.500 CPU sec, 2.521 real sec... sorting block 9, #reads = 1083886 mem = 536883409 peak = 885313536 [M::mem_process_seqs] Processed 697428 reads in 148.426 CPU sec, 4.722 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (54, 287247, 23, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 278, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (283.77, 176.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (272.98, 135.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (102, 403, 1591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4569) [M::mem_pestat] mean and std.dev: (866.43, 891.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6058) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 249, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (271.94, 150.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696324 sequences (100000147 bp) in 2.721 CPU sec, 2.859 real sec... sorting block 10, #reads = 1084079 mem = 536882601 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 287391, 24, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 213, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (233.74, 139.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1145) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (274.74, 135.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (300, 680, 1574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4122) [M::mem_pestat] mean and std.dev: (850.67, 930.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5396) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 232, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (254.61, 130.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697152 reads in 206.451 CPU sec, 6.609 real sec [M::process] read 695968 sequences (100000019 bp) in 2.335 CPU sec, 2.339 real sec... [M::mem_process_seqs] Processed 696442 reads in 134.737 CPU sec, 4.282 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 287522, 40, 108) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 269, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2276) [M::mem_pestat] mean and std.dev: (518.56, 519.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (275.14, 136.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (499, 748, 1469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3409) [M::mem_pestat] mean and std.dev: (991.31, 825.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4379) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 256, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (274.36, 150.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 11, #reads = 1084498 mem = 536882750 peak = 885313536 [M::process] read 703916 sequences (100000190 bp) in 4.069 CPU sec, 4.328 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 287134, 23, 94) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 278, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1970) [M::mem_pestat] mean and std.dev: (444.69, 398.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (168, 255, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (277.58, 137.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (342, 654, 1329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3303) [M::mem_pestat] mean and std.dev: (730.73, 525.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4290) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 286, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (262.76, 120.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696324 reads in 191.421 CPU sec, 6.095 real sec [M::mem_process_seqs] Processed 695968 reads in 136.958 CPU sec, 4.336 real sec [M::process] read 703592 sequences (100000083 bp) in 2.962 CPU sec, 3.087 real sec... sorting block 12, #reads = 1084437 mem = 536879676 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 289991, 24, 98) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 245, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1253) [M::mem_pestat] mean and std.dev: (330.06, 269.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 231, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (257.53, 130.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1192, 1532, 1605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (366, 2431) [M::mem_pestat] mean and std.dev: (1365.81, 241.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2844) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 374, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1360) [M::mem_pestat] mean and std.dev: (358.44, 199.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703336 sequences (100000209 bp) in 3.114 CPU sec, 3.293 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 289708, 28, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 244, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (246.89, 129.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 232, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (258.56, 131.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (307, 588, 1055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2551) [M::mem_pestat] mean and std.dev: (663.67, 510.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3299) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 267, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (269.48, 142.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703916 reads in 197.877 CPU sec, 6.339 real sec [M::mem_process_seqs] Processed 703592 reads in 135.504 CPU sec, 4.289 real sec sorting block 13, #reads = 1084550 mem = 536878882 peak = 885313536 [M::process] read 702814 sequences (100000178 bp) in 3.281 CPU sec, 3.491 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 289513, 25, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 233, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044) [M::mem_pestat] mean and std.dev: (277.27, 159.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 234, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (260.47, 132.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (308, 390, 1163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2873) [M::mem_pestat] mean and std.dev: (668.84, 492.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 341, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1410) [M::mem_pestat] mean and std.dev: (374.69, 217.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1810) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 289277, 17, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 324, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (317.71, 218.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 235, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (261.81, 133.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (195, 329, 5287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15471) [M::mem_pestat] mean and std.dev: (2297.24, 3391.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20563) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 309, 668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1636) [M::mem_pestat] mean and std.dev: (358.58, 302.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2120) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702422 sequences (100000023 bp) in 3.600 CPU sec, 3.793 real sec... [M::mem_process_seqs] Processed 703336 reads in 196.965 CPU sec, 6.393 real sec sorting block 14, #reads = 1088748 mem = 536878907 peak = 885313536 [M::mem_process_seqs] Processed 702814 reads in 139.950 CPU sec, 4.519 real sec [M::process] read 701726 sequences (100000237 bp) in 3.645 CPU sec, 3.914 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 289480, 12, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 254, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (248.67, 120.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 237, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (263.79, 134.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (104, 664, 2377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6923) [M::mem_pestat] mean and std.dev: (1371.50, 1888.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9196) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 299, 872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2204) [M::mem_pestat] mean and std.dev: (574.68, 542.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 15, #reads = 1087961 mem = 536880539 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 288957, 11, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 241, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2173) [M::mem_pestat] mean and std.dev: (400.20, 388.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (158, 239, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (267.10, 137.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (226, 685, 957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2419) [M::mem_pestat] mean and std.dev: (656.45, 477.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 327, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (310.51, 184.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1348) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702422 reads in 196.838 CPU sec, 6.336 real sec [M::process] read 701118 sequences (100000218 bp) in 3.860 CPU sec, 4.203 real sec... [M::mem_process_seqs] Processed 701726 reads in 139.712 CPU sec, 4.450 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 288992, 31, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 335, 917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2407) [M::mem_pestat] mean and std.dev: (567.47, 581.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3152) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 242, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (269.72, 138.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 494, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1501) [M::mem_pestat] mean and std.dev: (484.32, 362.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1934) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 252, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (303.15, 135.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699980 sequences (100000183 bp) in 3.843 CPU sec, 4.183 real sec... sorting block 16, #reads = 1088180 mem = 536880183 peak = 885313536 [M::mem_process_seqs] Processed 701118 reads in 115.078 CPU sec, 3.841 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (111, 288379, 23, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 431, 1208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3274) [M::mem_pestat] mean and std.dev: (422.03, 400.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 246, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (272.39, 139.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 439, 1606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4328) [M::mem_pestat] mean and std.dev: (659.43, 579.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5689) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 284, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (304.54, 152.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698998 sequences (100000142 bp) in 3.341 CPU sec, 3.608 real sec... [M::mem_process_seqs] Processed 699980 reads in 113.175 CPU sec, 3.761 real sec sorting block 17, #reads = 1087649 mem = 536880268 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (101, 288642, 19, 103) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 299, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (278.22, 146.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 248, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (274.43, 139.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 971) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (300, 492, 1303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3309) [M::mem_pestat] mean and std.dev: (488.73, 381.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4312) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 267, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (251.07, 101.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698858 sequences (100000296 bp) in 2.864 CPU sec, 3.085 real sec... [M::mem_process_seqs] Processed 698998 reads in 112.079 CPU sec, 3.710 real sec sorting block 18, #reads = 1086017 mem = 536882547 peak = 885313536 [M::process] read 698572 sequences (100000278 bp) in 3.320 CPU sec, 3.530 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 288303, 14, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 255, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (293.83, 200.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 250, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (274.86, 139.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (359, 629, 1994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5264) [M::mem_pestat] mean and std.dev: (1055.43, 794.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6899) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 258, 472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020) [M::mem_pestat] mean and std.dev: (271.52, 121.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 288422, 21, 97) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 241, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (236.04, 98.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 249, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (274.14, 138.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (239, 860, 1196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3110) [M::mem_pestat] mean and std.dev: (805.25, 597.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4067) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 286, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894) [M::mem_pestat] mean and std.dev: (292.29, 134.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697676 sequences (100000277 bp) in 2.962 CPU sec, 3.316 real sec... [M::mem_process_seqs] Processed 698858 reads in 200.976 CPU sec, 6.422 real sec [M::mem_process_seqs] Processed 698572 reads in 141.701 CPU sec, 4.501 real sec sorting block 19, #reads = 1085060 mem = 536885022 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 287539, 33, 94) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 256, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1696) [M::mem_pestat] mean and std.dev: (382.51, 331.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2204) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 252, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (276.02, 138.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 604, 1601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4259) [M::mem_pestat] mean and std.dev: (941.09, 828.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5588) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 271, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (234.38, 104.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697290 sequences (100000159 bp) in 3.955 CPU sec, 4.245 real sec... [M::mem_process_seqs] Processed 697676 reads in 110.153 CPU sec, 3.655 real sec [M::process] read 697282 sequences (100000014 bp) in 3.131 CPU sec, 3.315 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 287793, 15, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 336, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1164) [M::mem_pestat] mean and std.dev: (323.71, 176.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 253, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (276.68, 138.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (372, 921, 2003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5265) [M::mem_pestat] mean and std.dev: (1201.73, 1058.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6896) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 329, 628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1430) [M::mem_pestat] mean and std.dev: (410.90, 277.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697290 reads in 111.534 CPU sec, 3.718 real sec sorting block 20, #reads = 1084622 mem = 536881108 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 286990, 18, 110) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 245, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (256.54, 131.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 253, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (276.96, 138.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (458, 931, 1097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2375) [M::mem_pestat] mean and std.dev: (823.94, 457.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3014) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 372, 1517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4173) [M::mem_pestat] mean and std.dev: (751.57, 954.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5501) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 696706 sequences (100000265 bp) in 3.955 CPU sec, 4.324 real sec... [M::mem_process_seqs] Processed 697282 reads in 114.380 CPU sec, 3.805 real sec sorting block 21, #reads = 1083789 mem = 536878389 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 287677, 37, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 241, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1057) [M::mem_pestat] mean and std.dev: (329.17, 237.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (276.03, 136.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (277, 878, 1743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4675) [M::mem_pestat] mean and std.dev: (988.91, 890.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6141) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 274, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (274.61, 108.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696706 reads in 116.631 CPU sec, 3.863 real sec [M::process] read 696778 sequences (100000275 bp) in 3.879 CPU sec, 4.177 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 287647, 21, 109) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 304, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1668) [M::mem_pestat] mean and std.dev: (413.89, 379.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2171) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 252, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (274.54, 135.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 319, 1096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3222) [M::mem_pestat] mean and std.dev: (621.43, 753.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4285) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 331, 765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1875) [M::mem_pestat] mean and std.dev: (496.79, 438.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2430) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 696778 reads in 114.623 CPU sec, 3.809 real sec [M::process] read 697408 sequences (100000168 bp) in 3.798 CPU sec, 4.118 real sec... sorting block 22, #reads = 1085050 mem = 536879883 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 287339, 25, 112) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 296, 971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2579) [M::mem_pestat] mean and std.dev: (563.34, 615.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (273.64, 136.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (513, 816, 1463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3363) [M::mem_pestat] mean and std.dev: (937.62, 616.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4313) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 341, 624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1386) [M::mem_pestat] mean and std.dev: (382.48, 246.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697594 sequences (100000181 bp) in 3.536 CPU sec, 3.781 real sec... [M::mem_process_seqs] Processed 697408 reads in 117.014 CPU sec, 3.863 real sec sorting block 23, #reads = 1084678 mem = 536879555 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 287941, 27, 89) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 212, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867) [M::mem_pestat] mean and std.dev: (260.24, 159.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (272.80, 135.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (551, 800, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2108) [M::mem_pestat] mean and std.dev: (619.18, 398.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2627) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 252, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (247.70, 129.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697594 reads in 115.193 CPU sec, 3.797 real sec [M::process] read 697302 sequences (100000143 bp) in 3.656 CPU sec, 3.967 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 287612, 24, 93) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 285, 888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2362) [M::mem_pestat] mean and std.dev: (552.89, 591.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (166, 250, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (273.16, 135.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (477, 1746, 4089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11313) [M::mem_pestat] mean and std.dev: (2187.62, 1966.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14925) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 286, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (268.07, 113.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697302 reads in 116.098 CPU sec, 3.829 real sec sorting block 24, #reads = 1083571 mem = 536880058 peak = 885313536 [M::process] read 697274 sequences (100000048 bp) in 3.862 CPU sec, 4.159 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 287221, 19, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (164, 276, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1103) [M::mem_pestat] mean and std.dev: (315.43, 205.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (273.05, 135.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 447, 859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2261) [M::mem_pestat] mean and std.dev: (624.11, 547.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2962) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 284, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (311.71, 170.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1235) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697274 reads in 118.342 CPU sec, 3.884 real sec [M::process] read 696658 sequences (100000097 bp) in 3.776 CPU sec, 4.060 real sec... sorting block 25, #reads = 1083969 mem = 536877818 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (73, 286976, 23, 108) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 336, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1415) [M::mem_pestat] mean and std.dev: (392.74, 295.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (167, 252, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (275.51, 137.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (345, 458, 1885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4965) [M::mem_pestat] mean and std.dev: (866.87, 759.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6505) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 307, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271) [M::mem_pestat] mean and std.dev: (348.89, 237.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703648 sequences (100000070 bp) in 3.668 CPU sec, 3.930 real sec... [M::mem_process_seqs] Processed 696658 reads in 119.753 CPU sec, 4.099 real sec sorting block 26, #reads = 1082873 mem = 536879232 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 289775, 33, 93) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 199, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (231.39, 131.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 232, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (258.51, 131.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 899) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (216, 925, 1655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4533) [M::mem_pestat] mean and std.dev: (923.33, 819.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5972) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 315, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (293.18, 139.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703648 reads in 117.115 CPU sec, 3.791 real sec [M::process] read 704388 sequences (100000197 bp) in 3.622 CPU sec, 3.911 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 289800, 28, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 296, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1836) [M::mem_pestat] mean and std.dev: (445.86, 381.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 231, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (257.81, 131.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (307, 599, 1387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3547) [M::mem_pestat] mean and std.dev: (729.67, 546.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4627) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 281, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015) [M::mem_pestat] mean and std.dev: (296.77, 170.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703344 sequences (100000144 bp) in 3.535 CPU sec, 3.789 real sec... [M::mem_process_seqs] Processed 704388 reads in 117.401 CPU sec, 3.809 real sec sorting block 27, #reads = 1086995 mem = 536878899 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 289861, 25, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 236, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (226.27, 124.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 235, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (260.66, 132.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (176, 440, 1116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2996) [M::mem_pestat] mean and std.dev: (474.90, 414.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3936) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 289, 791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2001) [M::mem_pestat] mean and std.dev: (429.15, 396.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2606) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703344 reads in 99.839 CPU sec, 3.233 real sec [M::process] read 702694 sequences (100000113 bp) in 3.759 CPU sec, 4.032 real sec... sorting block 28, #reads = 1088557 mem = 536878529 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 289517, 15, 75) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 262, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002) [M::mem_pestat] mean and std.dev: (274.67, 188.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 236, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (262.25, 132.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (355, 611, 864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1882) [M::mem_pestat] mean and std.dev: (637.93, 405.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2391) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 281, 1060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2786) [M::mem_pestat] mean and std.dev: (506.67, 492.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3649) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702408 sequences (100000034 bp) in 2.920 CPU sec, 3.070 real sec... [M::mem_process_seqs] Processed 702694 reads in 101.060 CPU sec, 3.328 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (90, 289617, 37, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 293, 711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1767) [M::mem_pestat] mean and std.dev: (378.35, 343.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 238, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (264.77, 135.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (294, 1223, 3264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9204) [M::mem_pestat] mean and std.dev: (2165.16, 2345.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12174) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 319, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1276) [M::mem_pestat] mean and std.dev: (339.91, 179.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1627) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702408 reads in 104.019 CPU sec, 3.358 real sec [M::process] read 701404 sequences (100000059 bp) in 3.600 CPU sec, 3.837 real sec... sorting block 29, #reads = 1087552 mem = 536877456 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (43, 289581, 18, 92) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 231, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (279.45, 196.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 241, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (266.95, 135.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (223, 1104, 1417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3805) [M::mem_pestat] mean and std.dev: (669.07, 536.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4999) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 316, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1405) [M::mem_pestat] mean and std.dev: (371.77, 285.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701404 reads in 110.959 CPU sec, 3.577 real sec [M::process] read 700770 sequences (100000134 bp) in 3.357 CPU sec, 3.597 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 288964, 24, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 284, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1254) [M::mem_pestat] mean and std.dev: (322.05, 233.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 243, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (270.07, 138.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (170, 869, 3909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11387) [M::mem_pestat] mean and std.dev: (1721.83, 1761.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15126) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 336, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1483) [M::mem_pestat] mean and std.dev: (380.71, 256.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1905) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 30, #reads = 1087727 mem = 536882155 peak = 885313536 [M::mem_process_seqs] Processed 700770 reads in 112.110 CPU sec, 3.620 real sec [M::process] read 699972 sequences (100000077 bp) in 3.960 CPU sec, 4.262 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 288817, 36, 90) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 327, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (312.17, 117.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (272.77, 139.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (508, 1111, 2085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5239) [M::mem_pestat] mean and std.dev: (1133.64, 880.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6816) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 300, 1023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2665) [M::mem_pestat] mean and std.dev: (515.23, 542.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3486) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699628 sequences (100000217 bp) in 2.990 CPU sec, 3.145 real sec... [M::mem_process_seqs] Processed 699972 reads in 107.968 CPU sec, 3.485 real sec sorting block 31, #reads = 1086049 mem = 536879715 peak = 885313536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (96, 288433, 23, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 326, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1316) [M::mem_pestat] mean and std.dev: (369.22, 257.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 247, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (272.88, 139.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (190, 301, 1663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4609) [M::mem_pestat] mean and std.dev: (1026.36, 1281.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6153) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 303, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1302) [M::mem_pestat] mean and std.dev: (337.53, 238.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1666) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699426 sequences (100000083 bp) in 3.438 CPU sec, 3.663 real sec... [M::mem_process_seqs] Processed 699628 reads in 111.783 CPU sec, 3.626 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (94, 288800, 27, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 235, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (259.24, 164.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 247, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (273.11, 139.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (153, 1038, 1479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4131) [M::mem_pestat] mean and std.dev: (848.22, 664.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5457) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 320, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (337.58, 162.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1259) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 32, #reads = 1086016 mem = 536879528 peak = 907599872 [M::mem_process_seqs] Processed 699426 reads in 111.312 CPU sec, 3.599 real sec [M::process] read 698548 sequences (100000049 bp) in 3.780 CPU sec, 4.083 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 288311, 23, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 222, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (238.86, 149.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 249, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (274.20, 138.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (613, 1133, 1379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2911) [M::mem_pestat] mean and std.dev: (855.53, 567.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3677) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 306, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2696) [M::mem_pestat] mean and std.dev: (589.20, 585.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3543) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698548 reads in 108.929 CPU sec, 3.517 real sec [M::process] read 699404 sequences (100000090 bp) in 3.802 CPU sec, 4.066 real sec... sorting block 33, #reads = 1084954 mem = 536882214 peak = 907599872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 288247, 24, 85) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 197, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980) [M::mem_pestat] mean and std.dev: (218.08, 120.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (271.77, 137.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (204, 649, 3289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9459) [M::mem_pestat] mean and std.dev: (1483.17, 1694.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 274, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (293.68, 172.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698724 sequences (100000050 bp) in 3.190 CPU sec, 3.382 real sec... [M::mem_process_seqs] Processed 699404 reads in 112.359 CPU sec, 3.630 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 288183, 23, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 265, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1434) [M::mem_pestat] mean and std.dev: (387.21, 326.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (272.78, 138.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (310, 513, 1409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3607) [M::mem_pestat] mean and std.dev: (745.05, 647.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 312, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1454) [M::mem_pestat] mean and std.dev: (333.82, 199.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 34, #reads = 1085915 mem = 536880885 peak = 907599872 [M::process] read 698908 sequences (100000007 bp) in 3.312 CPU sec, 3.545 real sec... [M::mem_process_seqs] Processed 698724 reads in 113.990 CPU sec, 3.669 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 288398, 21, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 278, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (274.54, 151.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1218) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 245, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (270.92, 136.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (338, 827, 2475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6749) [M::mem_pestat] mean and std.dev: (1126.75, 1139.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8886) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 372, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2215) [M::mem_pestat] mean and std.dev: (521.83, 429.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2890) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698908 reads in 106.249 CPU sec, 3.416 real sec [M::process] read 698642 sequences (100000254 bp) in 3.642 CPU sec, 3.884 real sec... sorting block 35, #reads = 1084652 mem = 536880292 peak = 907599872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (55, 287989, 28, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 200, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637) [M::mem_pestat] mean and std.dev: (219.92, 97.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 244, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (269.45, 135.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 1122, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7429) [M::mem_pestat] mean and std.dev: (1306.12, 1565.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9837) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 280, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (333.29, 218.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1571) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698642 reads in 98.732 CPU sec, 3.171 real sec [M::process] read 698712 sequences (100000213 bp) in 3.377 CPU sec, 3.621 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 288143, 22, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 250, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839) [M::mem_pestat] mean and std.dev: (300.19, 207.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 245, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (270.02, 135.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (197, 887, 1032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2702) [M::mem_pestat] mean and std.dev: (719.70, 632.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3537) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 273, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1430) [M::mem_pestat] mean and std.dev: (329.90, 289.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697950 sequences (100000126 bp) in 3.024 CPU sec, 3.176 real sec... [M::mem_process_seqs] Processed 698712 reads in 101.817 CPU sec, 3.305 real sec sorting block 36, #reads = 1084317 mem = 536879164 peak = 907599872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 287258, 25, 95) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 259, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (307.44, 174.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (270.96, 134.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (403, 1050, 2085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5449) [M::mem_pestat] mean and std.dev: (1126.58, 846.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7131) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 275, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (322.23, 165.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697950 reads in 100.568 CPU sec, 3.226 real sec [M::process] read 697912 sequences (100000031 bp) in 3.410 CPU sec, 3.622 real sec... sorting block 37, #reads = 1083060 mem = 536881133 peak = 907599872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 288112, 24, 90) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 241, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (243.97, 117.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (272.89, 136.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (606, 814, 1655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3753) [M::mem_pestat] mean and std.dev: (1072.75, 811.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 307, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1837) [M::mem_pestat] mean and std.dev: (442.06, 363.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697598 sequences (100000287 bp) in 2.659 CPU sec, 2.869 real sec... [M::mem_process_seqs] Processed 697912 reads in 97.216 CPU sec, 3.127 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 287708, 36, 90) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 282, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013) [M::mem_pestat] mean and std.dev: (265.71, 160.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 249, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (273.29, 136.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (146, 327, 1756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4976) [M::mem_pestat] mean and std.dev: (854.88, 1166.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6586) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 301, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1740) [M::mem_pestat] mean and std.dev: (462.86, 406.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2260) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697424 sequences (100000084 bp) in 2.960 CPU sec, 3.148 real sec... [M::mem_process_seqs] Processed 697598 reads in 96.255 CPU sec, 3.102 real sec sorting block 38, #reads = 1082873 mem = 536879069 peak = 907599872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 287148, 43, 96) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 218, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244) [M::mem_pestat] mean and std.dev: (322.77, 255.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (273.14, 135.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (253, 550, 1422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3760) [M::mem_pestat] mean and std.dev: (855.91, 826.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4929) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 315, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1204) [M::mem_pestat] mean and std.dev: (272.24, 135.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1546) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697486 sequences (100000032 bp) in 3.158 CPU sec, 3.318 real sec... [M::mem_process_seqs] Processed 697424 reads in 99.451 CPU sec, 3.199 real sec sorting block 39, #reads = 1083820 mem = 536884331 peak = 907599872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 287598, 32, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 269, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (286.05, 166.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 250, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (273.65, 136.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (103, 421, 1266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3592) [M::mem_pestat] mean and std.dev: (580.37, 611.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4755) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 299, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (297.39, 192.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697486 reads in 102.623 CPU sec, 3.289 real sec [M::process] read 704272 sequences (100000153 bp) in 3.267 CPU sec, 3.416 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 289370, 16, 115) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 284, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1157) [M::mem_pestat] mean and std.dev: (296.22, 161.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 231, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (257.94, 131.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 902) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (169, 265, 1223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3331) [M::mem_pestat] mean and std.dev: (592.67, 569.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4385) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 297, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1130) [M::mem_pestat] mean and std.dev: (325.30, 190.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704002 sequences (100000047 bp) in 2.822 CPU sec, 2.950 real sec... [M::mem_process_seqs] Processed 704272 reads in 99.842 CPU sec, 3.207 real sec sorting block 40, #reads = 1083600 mem = 536880849 peak = 907599872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 289444, 23, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 232, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010) [M::mem_pestat] mean and std.dev: (320.96, 242.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1291) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 233, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (260.30, 133.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (189, 598, 3620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10482) [M::mem_pestat] mean and std.dev: (1455.17, 1715.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13913) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 340, 806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2002) [M::mem_pestat] mean and std.dev: (470.57, 466.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2600) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702940 sequences (100000276 bp) in 2.912 CPU sec, 3.117 real sec... [M::mem_process_seqs] Processed 704002 reads in 98.521 CPU sec, 3.171 real sec sorting block 41, #reads = 1089528 mem = 536884317 peak = 907599872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 289552, 17, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 290, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (322.96, 196.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 236, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (262.07, 133.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (166, 433, 4379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12805) [M::mem_pestat] mean and std.dev: (1769.59, 2392.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17018) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 242, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (266.71, 132.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1254) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702130 sequences (100000225 bp) in 3.052 CPU sec, 3.227 real sec... [M::mem_process_seqs] Processed 702940 reads in 98.091 CPU sec, 3.148 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 289807, 20, 87) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (304.84, 200.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 238, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (264.67, 135.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (458, 505, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1283) [M::mem_pestat] mean and std.dev: (441.81, 147.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1558) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 291, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231) [M::mem_pestat] mean and std.dev: (312.49, 198.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1585) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701176 sequences (100000251 bp) in 2.768 CPU sec, 2.873 real sec... [M::mem_process_seqs] Processed 702130 reads in 96.621 CPU sec, 3.097 real sec sorting block 42, #reads = 1089029 mem = 536879241 peak = 907599872 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 288643, 17, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 207, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (318.99, 251.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 242, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (268.16, 137.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (104, 701, 1831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5285) [M::mem_pestat] mean and std.dev: (1050.76, 1168.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7012) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 346, 1286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3380) [M::mem_pestat] mean and std.dev: (583.31, 619.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700700 sequences (100000184 bp) in 2.766 CPU sec, 2.878 real sec... [M::mem_process_seqs] Processed 701176 reads in 96.143 CPU sec, 3.071 real sec sorting block 43, #reads = 1087863 mem = 536879414 peak = 907825152 [M::process] read 700452 sequences (100000186 bp) in 2.691 CPU sec, 2.798 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (71, 288699, 27, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 276, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1144) [M::mem_pestat] mean and std.dev: (340.82, 216.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 243, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (269.06, 137.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (191, 397, 2690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7688) [M::mem_pestat] mean and std.dev: (1886.74, 2515.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11948) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 303, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (311.47, 175.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700070 sequences (100000208 bp) in 2.069 CPU sec, 2.067 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 288610, 32, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 208, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909) [M::mem_pestat] mean and std.dev: (276.57, 193.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 243, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (269.44, 137.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (906, 1317, 2882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6834) [M::mem_pestat] mean and std.dev: (1515.33, 1126.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8810) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 325, 740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1872) [M::mem_pestat] mean and std.dev: (369.19, 303.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2438) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700700 reads in 171.345 CPU sec, 5.379 real sec [M::mem_process_seqs] Processed 700452 reads in 125.047 CPU sec, 3.929 real sec [M::process] read 699394 sequences (100000053 bp) in 2.545 CPU sec, 2.656 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (61, 289011, 8, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 285, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1640) [M::mem_pestat] mean and std.dev: (397.54, 348.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2122) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (272.02, 139.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 286, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (265.12, 95.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR sorting block 44, #reads = 1087058 mem = 536877364 peak = 907825152 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 288412, 29, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 277, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429) [M::mem_pestat] mean and std.dev: (324.95, 263.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::process] read 699858 sequences (100000256 bp) in 3.348 CPU sec, 3.588 real sec... [M::mem_pestat] (25, 50, 75) percentile: (162, 245, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (272.62, 139.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (850, 1788, 3068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7504) [M::mem_pestat] mean and std.dev: (1939.41, 1533.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9722) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 261, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206) [M::mem_pestat] mean and std.dev: (317.24, 213.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700070 reads in 186.614 CPU sec, 5.979 real sec [M::mem_process_seqs] Processed 699394 reads in 133.383 CPU sec, 4.219 real sec sorting block 45, #reads = 1086208 mem = 536879776 peak = 910909440 [M::process] read 699268 sequences (100000146 bp) in 2.755 CPU sec, 2.902 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (106, 288426, 13, 107) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 286, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (282.79, 151.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1060) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 245, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (272.03, 139.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (381, 906, 4011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11271) [M::mem_pestat] mean and std.dev: (1429.31, 1507.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14901) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 267, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (246.60, 103.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699952 sequences (100000194 bp) in 2.725 CPU sec, 3.060 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (59, 288139, 20, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 237, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (273.36, 140.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 244, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (271.04, 137.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (712, 1243, 1748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3820) [M::mem_pestat] mean and std.dev: (1037.74, 581.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4856) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 409, 729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1809) [M::mem_pestat] mean and std.dev: (447.49, 352.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2349) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700162 sequences (100000254 bp) in 1.539 CPU sec, 1.538 real sec... [M::mem_process_seqs] Processed 699858 reads in 189.687 CPU sec, 6.033 real sec sorting block 46, #reads = 1086429 mem = 536880387 peak = 939229184 [M::mem_process_seqs] Processed 699268 reads in 132.739 CPU sec, 4.209 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 288719, 27, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (155, 215, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1292) [M::mem_pestat] mean and std.dev: (330.12, 276.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 242, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 746) [M::mem_pestat] mean and std.dev: (269.14, 137.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 567, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2305) [M::mem_pestat] mean and std.dev: (453.50, 414.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3050) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 369, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1751) [M::mem_pestat] mean and std.dev: (358.88, 245.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2272) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 47, #reads = 1085967 mem = 536880016 peak = 969453568 [M::process] read 699938 sequences (100000133 bp) in 2.921 CPU sec, 3.030 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 288627, 28, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 267, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (269.43, 144.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (268.36, 136.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (190, 692, 1275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3445) [M::mem_pestat] mean and std.dev: (699.58, 626.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 300, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1494) [M::mem_pestat] mean and std.dev: (373.29, 273.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1936) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699952 reads in 191.284 CPU sec, 6.063 real sec [M::process] read 700136 sequences (100000267 bp) in 2.398 CPU sec, 2.397 real sec... [M::mem_process_seqs] Processed 700162 reads in 133.603 CPU sec, 4.217 real sec sorting block 48, #reads = 1087082 mem = 536878136 peak = 969453568 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 288526, 13, 113) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 292, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1164) [M::mem_pestat] mean and std.dev: (300.59, 205.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (267.17, 135.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (219, 638, 2324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6534) [M::mem_pestat] mean and std.dev: (946.92, 943.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8639) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 247, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (267.14, 120.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699264 sequences (100000078 bp) in 2.977 CPU sec, 3.127 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 288384, 21, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 294, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2034) [M::mem_pestat] mean and std.dev: (416.52, 351.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 240, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (267.01, 135.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (403, 1087, 2461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6577) [M::mem_pestat] mean and std.dev: (1457.95, 1334.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8635) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 285, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (309.39, 153.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698964 sequences (100000084 bp) in 2.169 CPU sec, 2.175 real sec... [M::mem_process_seqs] Processed 699938 reads in 194.353 CPU sec, 6.169 real sec [M::mem_process_seqs] Processed 700136 reads in 142.898 CPU sec, 4.539 real sec sorting block 49, #reads = 1086747 mem = 536883253 peak = 969453568 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 287986, 24, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 284, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1147) [M::mem_pestat] mean and std.dev: (303.89, 207.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (267.62, 134.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (580, 756, 1088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2104) [M::mem_pestat] mean and std.dev: (745.62, 320.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2612) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 389, 1034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2714) [M::mem_pestat] mean and std.dev: (590.88, 562.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698472 sequences (100000068 bp) in 3.113 CPU sec, 3.227 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (104, 288420, 15, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 270, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (286.27, 158.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 243, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (268.22, 134.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (130, 342, 721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1903) [M::mem_pestat] mean and std.dev: (314.69, 260.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2494) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 242, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (252.54, 108.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699264 reads in 192.644 CPU sec, 6.115 real sec [M::process] read 698478 sequences (100000027 bp) in 2.634 CPU sec, 2.676 real sec... [M::mem_process_seqs] Processed 698964 reads in 133.292 CPU sec, 4.206 real sec sorting block 50, #reads = 1085023 mem = 536881252 peak = 969453568 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (98, 288084, 28, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 287, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (260.10, 113.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (270.38, 135.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (103, 1208, 2806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8212) [M::mem_pestat] mean and std.dev: (1751.21, 1748.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10915) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 336, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (305.23, 145.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698188 sequences (100000158 bp) in 3.220 CPU sec, 3.323 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 288160, 23, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 342, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2386) [M::mem_pestat] mean and std.dev: (539.84, 606.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (271.37, 136.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (146, 452, 1391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3881) [M::mem_pestat] mean and std.dev: (614.26, 805.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5126) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 266, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (291.15, 144.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698472 reads in 188.948 CPU sec, 5.995 real sec [M::process] read 697626 sequences (100000249 bp) in 2.492 CPU sec, 2.511 real sec... sorting block 51, #reads = 1085403 mem = 536880243 peak = 969453568 [M::mem_process_seqs] Processed 698478 reads in 132.282 CPU sec, 4.171 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 287834, 31, 88) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 298, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (296.97, 182.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 248, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (272.28, 136.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (565, 709, 1921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4633) [M::mem_pestat] mean and std.dev: (1441.77, 1343.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6815) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 288, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1387) [M::mem_pestat] mean and std.dev: (374.95, 255.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 52, #reads = 1084467 mem = 536881105 peak = 1027137536 [M::process] read 707518 sequences (100000061 bp) in 3.442 CPU sec, 3.603 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 287540, 37, 108) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 265, 1313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3597) [M::mem_pestat] mean and std.dev: (599.73, 668.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (165, 248, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (272.23, 135.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (233, 916, 1545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4169) [M::mem_pestat] mean and std.dev: (1005.33, 910.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5481) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 298, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199) [M::mem_pestat] mean and std.dev: (373.32, 252.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698188 reads in 189.569 CPU sec, 6.030 real sec [M::mem_process_seqs] Processed 697626 reads in 133.637 CPU sec, 4.207 real sec [M::process] read 708958 sequences (100000276 bp) in 3.181 CPU sec, 3.283 real sec... sorting block 53, #reads = 1084509 mem = 536876875 peak = 1027137536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (50, 290523, 19, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 244, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 950) [M::mem_pestat] mean and std.dev: (287.53, 211.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 221, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (249.09, 128.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (357, 808, 1306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3204) [M::mem_pestat] mean and std.dev: (794.61, 669.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4153) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 223, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (240.43, 107.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706762 sequences (100000238 bp) in 3.222 CPU sec, 3.382 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 291865, 20, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 298, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1299) [M::mem_pestat] mean and std.dev: (319.30, 213.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 218, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (246.24, 127.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (309, 816, 3260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9162) [M::mem_pestat] mean and std.dev: (1528.68, 1616.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12113) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 258, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (300.23, 160.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 707518 reads in 207.503 CPU sec, 6.599 real sec [M::process] read 706470 sequences (100000117 bp) in 2.545 CPU sec, 2.583 real sec... [M::mem_process_seqs] Processed 708958 reads in 137.943 CPU sec, 4.346 real sec sorting block 54, #reads = 1084104 mem = 536886436 peak = 1027137536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (72, 290803, 32, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 251, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908) [M::mem_pestat] mean and std.dev: (250.75, 139.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 222, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (251.83, 130.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (789, 964, 1277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2253) [M::mem_pestat] mean and std.dev: (927.21, 476.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2831) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 247, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (276.79, 140.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1042) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706958 sequences (100000301 bp) in 3.386 CPU sec, 3.582 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 291344, 28, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 359, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1425) [M::mem_pestat] mean and std.dev: (390.95, 273.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 224, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (253.78, 132.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (284, 433, 1652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4388) [M::mem_pestat] mean and std.dev: (894.18, 843.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5756) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 280, 850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2180) [M::mem_pestat] mean and std.dev: (483.29, 462.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2845) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 706762 reads in 197.542 CPU sec, 6.275 real sec [M::mem_process_seqs] Processed 706470 reads in 139.371 CPU sec, 4.383 real sec sorting block 55, #reads = 1087991 mem = 536885888 peak = 1027137536 [M::process] read 705068 sequences (100000051 bp) in 2.807 CPU sec, 2.887 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 291118, 22, 78) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 315, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (294.29, 155.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 221, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (249.42, 128.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (447, 1247, 3709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10233) [M::mem_pestat] mean and std.dev: (2119.59, 2051.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13495) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 306, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (356.70, 236.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 56, #reads = 1087784 mem = 536880166 peak = 1027137536 [M::process] read 704720 sequences (100000298 bp) in 3.313 CPU sec, 3.483 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 290454, 21, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 236, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1950) [M::mem_pestat] mean and std.dev: (380.25, 346.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 228, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (256.98, 133.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (497, 825, 1533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3605) [M::mem_pestat] mean and std.dev: (859.79, 548.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4641) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 347, 1557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4259) [M::mem_pestat] mean and std.dev: (850.45, 1039.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5610) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 706958 reads in 200.766 CPU sec, 6.414 real sec [M::mem_process_seqs] Processed 705068 reads in 141.123 CPU sec, 4.453 real sec [M::process] read 702688 sequences (100000132 bp) in 3.111 CPU sec, 3.244 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 290636, 28, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 259, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (264.52, 157.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1216) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 229, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (259.37, 135.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (453, 651, 1877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4725) [M::mem_pestat] mean and std.dev: (1088.21, 972.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6149) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 232, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999) [M::mem_pestat] mean and std.dev: (275.35, 160.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 57, #reads = 1088806 mem = 536877785 peak = 1027137536 [M::process] read 703794 sequences (100000052 bp) in 3.793 CPU sec, 4.029 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (108, 289573, 20, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 258, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (279.70, 156.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 235, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (265.20, 138.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (583, 1248, 2043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4963) [M::mem_pestat] mean and std.dev: (1312.40, 839.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6423) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 237, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (290.79, 159.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704720 reads in 200.327 CPU sec, 6.401 real sec [M::mem_process_seqs] Processed 702688 reads in 144.484 CPU sec, 4.572 real sec sorting block 58, #reads = 1087910 mem = 536880420 peak = 1027137536 [M::process] read 704758 sequences (100000167 bp) in 3.632 CPU sec, 3.869 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (89, 290483, 30, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 245, 726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1876) [M::mem_pestat] mean and std.dev: (358.30, 337.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 232, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (262.78, 137.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (345, 564, 1562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3996) [M::mem_pestat] mean and std.dev: (863.18, 745.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5213) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 253, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (269.57, 146.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 290705, 19, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 232, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1487) [M::mem_pestat] mean and std.dev: (378.07, 341.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1935) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 226, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (254.52, 130.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (224, 711, 2665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7547) [M::mem_pestat] mean and std.dev: (1467.95, 1703.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 377, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (384.61, 230.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1567) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701896 sequences (100000293 bp) in 3.283 CPU sec, 3.524 real sec... [M::mem_process_seqs] Processed 703794 reads in 202.201 CPU sec, 6.472 real sec [M::mem_process_seqs] Processed 704758 reads in 143.450 CPU sec, 4.542 real sec sorting block 59, #reads = 1084925 mem = 536878662 peak = 1027137536 [M::process] read 702752 sequences (100000231 bp) in 3.203 CPU sec, 3.380 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 289254, 16, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 223, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (280.03, 170.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1247) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 237, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (266.82, 138.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (322, 667, 1932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5152) [M::mem_pestat] mean and std.dev: (850.87, 730.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6762) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 287, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (292.00, 150.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1158) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 60, #reads = 1087987 mem = 536878713 peak = 1027137536 [M::process] read 701160 sequences (100000239 bp) in 3.468 CPU sec, 3.687 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (112, 289709, 23, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 300, 487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (326.21, 137.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1168) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 235, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (264.62, 137.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (409, 838, 1908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4906) [M::mem_pestat] mean and std.dev: (931.43, 906.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6405) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 293, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 962) [M::mem_pestat] mean and std.dev: (300.92, 154.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701896 reads in 203.951 CPU sec, 6.512 real sec [M::mem_process_seqs] Processed 702752 reads in 143.955 CPU sec, 4.555 real sec [M::process] read 699468 sequences (100000122 bp) in 3.423 CPU sec, 3.616 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 288678, 16, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (165, 222, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (316.41, 263.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 241, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (269.50, 139.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (549, 778, 1856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4470) [M::mem_pestat] mean and std.dev: (794.69, 465.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5777) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 231, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (267.69, 145.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 61, #reads = 1085546 mem = 536882592 peak = 1027137536 [M::process] read 698416 sequences (100000032 bp) in 3.401 CPU sec, 3.597 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (57, 288368, 26, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 313, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2363) [M::mem_pestat] mean and std.dev: (469.37, 452.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3082) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (272.80, 139.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (344, 1584, 3555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9977) [M::mem_pestat] mean and std.dev: (1777.42, 1329.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13188) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 278, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828) [M::mem_pestat] mean and std.dev: (299.44, 139.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701160 reads in 206.821 CPU sec, 6.586 real sec [M::mem_process_seqs] Processed 699468 reads in 144.476 CPU sec, 4.559 real sec [M::process] read 698024 sequences (100000142 bp) in 3.535 CPU sec, 3.729 real sec... sorting block 62, #reads = 1083725 mem = 536877180 peak = 1027137536 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (98, 288072, 38, 91) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 322, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1945) [M::mem_pestat] mean and std.dev: (460.28, 416.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 247, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (273.58, 138.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (287, 933, 1509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3953) [M::mem_pestat] mean and std.dev: (1056.00, 1034.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5193) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 299, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916) [M::mem_pestat] mean and std.dev: (297.49, 163.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697878 sequences (100000264 bp) in 3.287 CPU sec, 3.516 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 287980, 13, 80) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 317, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868) [M::mem_pestat] mean and std.dev: (383.82, 284.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 249, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (274.71, 138.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (80, 409, 1228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3524) [M::mem_pestat] mean and std.dev: (552.25, 664.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 297, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (327.00, 207.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1343) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698416 reads in 201.550 CPU sec, 6.423 real sec [M::mem_process_seqs] Processed 698024 reads in 141.890 CPU sec, 4.482 real sec sorting block 63, #reads = 1082622 mem = 536881587 peak = 1027137536 [M::process] read 697680 sequences (100000030 bp) in 3.181 CPU sec, 3.335 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (107, 288144, 29, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 286, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1558) [M::mem_pestat] mean and std.dev: (396.92, 310.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2014) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 249, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (274.77, 138.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (470, 831, 1856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4628) [M::mem_pestat] mean and std.dev: (1087.21, 1055.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6014) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 286, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (293.22, 182.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 697716 sequences (100000113 bp) in 3.153 CPU sec, 3.307 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 288295, 32, 63) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 402, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1364) [M::mem_pestat] mean and std.dev: (358.39, 211.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 249, 365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767) [M::mem_pestat] mean and std.dev: (275.40, 139.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (471, 667, 1233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2757) [M::mem_pestat] mean and std.dev: (936.91, 661.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3581) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 253, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896) [M::mem_pestat] mean and std.dev: (277.14, 153.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 697878 reads in 200.963 CPU sec, 6.388 real sec sorting block 64, #reads = 1081249 mem = 536880639 peak = 1027137536 [M::mem_process_seqs] Processed 697680 reads in 140.342 CPU sec, 4.425 real sec [M::process] read 709118 sequences (100000279 bp) in 3.062 CPU sec, 3.176 real sec... sorting block 65, #reads = 1081255 mem = 536883419 peak = 1027137536 [M::process] read 710934 sequences (100000120 bp) in 3.471 CPU sec, 3.611 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (86, 291858, 26, 77) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 267, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (309.01, 195.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 218, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (246.37, 127.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (172, 616, 2132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6052) [M::mem_pestat] mean and std.dev: (1000.81, 903.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8012) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 282, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (279.97, 168.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1405) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 709118 reads in 124.263 CPU sec, 3.899 real sec [M::process] read 709598 sequences (100000101 bp) in 2.517 CPU sec, 2.544 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 287823, 28, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 228, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981) [M::mem_pestat] mean and std.dev: (288.23, 184.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1246) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (164, 250, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (276.80, 140.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (87, 632, 1978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5760) [M::mem_pestat] mean and std.dev: (1023.96, 1150.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7651) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 373, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1108) [M::mem_pestat] mean and std.dev: (350.57, 218.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1410) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 292025, 23, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 271, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2260) [M::mem_pestat] mean and std.dev: (503.50, 413.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (145, 213, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (240.28, 122.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (296, 771, 1057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2579) [M::mem_pestat] mean and std.dev: (665.00, 433.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 297, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1399) [M::mem_pestat] mean and std.dev: (331.53, 242.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1807) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707356 sequences (100000106 bp) in 2.723 CPU sec, 2.726 real sec... [M::mem_process_seqs] Processed 697716 reads in 336.685 CPU sec, 10.652 real sec sorting block 66, #reads = 1082762 mem = 536879044 peak = 1027137536 [M::mem_process_seqs] Processed 710934 reads in 184.719 CPU sec, 5.851 real sec sorting block 67, #reads = 1082575 mem = 536885038 peak = 1036898304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (82, 291582, 26, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 333, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1315) [M::mem_pestat] mean and std.dev: (405.88, 293.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 216, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (243.84, 124.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (132, 336, 2332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6732) [M::mem_pestat] mean and std.dev: (1031.88, 1284.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8932) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 322, 730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1792) [M::mem_pestat] mean and std.dev: (471.08, 380.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2323) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706036 sequences (100000108 bp) in 3.463 CPU sec, 3.722 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 291242, 16, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 277, 481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123) [M::mem_pestat] mean and std.dev: (299.36, 203.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 221, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (249.18, 127.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 865) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 472, 1101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3205) [M::mem_pestat] mean and std.dev: (474.21, 683.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 354, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (343.34, 207.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705016 sequences (100000053 bp) in 2.100 CPU sec, 2.097 real sec... [M::mem_process_seqs] Processed 709598 reads in 219.059 CPU sec, 6.952 real sec [M::mem_process_seqs] Processed 707356 reads in 153.967 CPU sec, 4.904 real sec sorting block 68, #reads = 1082555 mem = 536878858 peak = 1036898304 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 290780, 21, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 248, 558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1328) [M::mem_pestat] mean and std.dev: (294.95, 195.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 225, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (253.38, 130.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (208, 730, 2964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8476) [M::mem_pestat] mean and std.dev: (1965.00, 2445.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11746) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 231, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093) [M::mem_pestat] mean and std.dev: (292.13, 209.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1397) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704462 sequences (100000228 bp) in 3.191 CPU sec, 3.338 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 290101, 25, 100) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 338, 1696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4702) [M::mem_pestat] mean and std.dev: (784.56, 955.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6205) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 228, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (256.84, 132.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 901) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (105, 357, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (304.83, 235.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1649) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 242, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1557) [M::mem_pestat] mean and std.dev: (369.22, 328.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2021) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703998 sequences (100000223 bp) in 2.640 CPU sec, 2.641 real sec... [M::mem_process_seqs] Processed 706036 reads in 216.974 CPU sec, 6.865 real sec sorting block 69, #reads = 1082510 mem = 536879843 peak = 1036898304 [M::mem_process_seqs] Processed 705016 reads in 146.091 CPU sec, 4.615 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (88, 289751, 22, 98) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 318, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1205) [M::mem_pestat] mean and std.dev: (351.89, 212.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 229, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (258.75, 133.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (249, 849, 2018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5556) [M::mem_pestat] mean and std.dev: (1393.71, 1782.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8525) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 267, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (257.51, 125.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1079) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703244 sequences (100000204 bp) in 3.349 CPU sec, 3.540 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (81, 289725, 23, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 256, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017) [M::mem_pestat] mean and std.dev: (290.14, 195.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 231, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (260.62, 134.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (665, 1040, 1698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3764) [M::mem_pestat] mean and std.dev: (1131.59, 763.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 345, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1362) [M::mem_pestat] mean and std.dev: (401.55, 271.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1736) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702918 sequences (100000057 bp) in 2.510 CPU sec, 2.505 real sec... [M::mem_process_seqs] Processed 704462 reads in 209.859 CPU sec, 6.634 real sec sorting block 70, #reads = 1083342 mem = 536882537 peak = 1036898304 [M::mem_process_seqs] Processed 703998 reads in 155.993 CPU sec, 4.934 real sec sorting block 71, #reads = 1081857 mem = 536879868 peak = 1053782016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 290086, 27, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 254, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (296.44, 206.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 233, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (261.70, 134.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1103, 1591, 1591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 2567) [M::mem_pestat] mean and std.dev: (1287.39, 526.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3394) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 342, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101) [M::mem_pestat] mean and std.dev: (318.48, 215.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1412) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702196 sequences (100000157 bp) in 3.195 CPU sec, 3.358 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 289921, 23, 118) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 258, 627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1555) [M::mem_pestat] mean and std.dev: (358.17, 334.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 234, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (262.37, 134.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (497, 909, 1686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4064) [M::mem_pestat] mean and std.dev: (1062.35, 785.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5253) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 330, 623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1491) [M::mem_pestat] mean and std.dev: (388.32, 289.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1925) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702104 sequences (100000012 bp) in 2.652 CPU sec, 2.652 real sec... [M::mem_process_seqs] Processed 703244 reads in 207.686 CPU sec, 6.584 real sec [M::mem_process_seqs] Processed 702918 reads in 154.120 CPU sec, 4.908 real sec sorting block 72, #reads = 1082918 mem = 536876712 peak = 1053782016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 289813, 29, 110) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 244, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (253.56, 175.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 237, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (265.07, 136.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (306, 846, 1960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5268) [M::mem_pestat] mean and std.dev: (962.82, 854.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6922) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 293, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1773) [M::mem_pestat] mean and std.dev: (427.00, 365.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701042 sequences (100000085 bp) in 3.101 CPU sec, 3.302 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 289411, 22, 110) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 225, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (259.27, 132.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 236, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (265.11, 136.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (412, 772, 4564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12868) [M::mem_pestat] mean and std.dev: (2199.27, 2245.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17020) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 290, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 955) [M::mem_pestat] mean and std.dev: (322.11, 167.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699302 sequences (100000042 bp) in 1.980 CPU sec, 1.988 real sec... [M::mem_process_seqs] Processed 702196 reads in 206.675 CPU sec, 6.564 real sec [M::mem_process_seqs] Processed 702104 reads in 143.074 CPU sec, 4.535 real sec sorting block 73, #reads = 1083486 mem = 536891675 peak = 1053782016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (104, 288717, 27, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 286, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (292.79, 138.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 242, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (269.91, 139.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (103, 162, 530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1384) [M::mem_pestat] mean and std.dev: (282.04, 313.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1811) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 227, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (251.14, 126.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 698844 sequences (100000283 bp) in 3.190 CPU sec, 3.337 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 288475, 29, 106) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 286, 1004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2668) [M::mem_pestat] mean and std.dev: (557.09, 641.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 247, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (275.08, 141.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (735, 1428, 4746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12768) [M::mem_pestat] mean and std.dev: (2348.00, 1876.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16779) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 320, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1281) [M::mem_pestat] mean and std.dev: (337.93, 213.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701042 reads in 197.032 CPU sec, 6.238 real sec [M::process] read 699270 sequences (100000249 bp) in 3.087 CPU sec, 3.137 real sec... [M::mem_process_seqs] Processed 699302 reads in 138.301 CPU sec, 4.343 real sec sorting block 74, #reads = 1083299 mem = 536880140 peak = 1053782016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 288374, 18, 81) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 322, 958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2516) [M::mem_pestat] mean and std.dev: (580.58, 594.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (163, 247, 364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (274.46, 140.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (565, 2304, 2619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6727) [M::mem_pestat] mean and std.dev: (2227.94, 1862.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9678) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 306, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (287.43, 127.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 75, #reads = 1083037 mem = 536880426 peak = 1053782016 [M::process] read 699090 sequences (100000200 bp) in 3.218 CPU sec, 3.364 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (94, 288291, 24, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 257, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159) [M::mem_pestat] mean and std.dev: (311.99, 210.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (273.26, 141.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 552, 2048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5604) [M::mem_pestat] mean and std.dev: (1101.04, 1382.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 268, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (275.30, 156.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 698844 reads in 200.186 CPU sec, 6.348 real sec [M::process] read 700468 sequences (100000189 bp) in 2.791 CPU sec, 2.827 real sec... [M::mem_process_seqs] Processed 699270 reads in 141.359 CPU sec, 4.444 real sec sorting block 76, #reads = 1082529 mem = 536878149 peak = 1053782016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (85, 288215, 17, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 202, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (237.63, 132.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (162, 244, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (272.86, 140.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (531, 1756, 3779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10275) [M::mem_pestat] mean and std.dev: (2213.53, 2292.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13523) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 288, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1434) [M::mem_pestat] mean and std.dev: (358.00, 279.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704876 sequences (100000247 bp) in 2.995 CPU sec, 3.113 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 289025, 26, 124) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 236, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (240.46, 104.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 239, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (267.93, 138.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (320, 916, 1573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4079) [M::mem_pestat] mean and std.dev: (985.79, 675.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5332) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 284, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (309.14, 157.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1279) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 709284 sequences (100000095 bp) in 2.090 CPU sec, 2.092 real sec... [M::mem_process_seqs] Processed 699090 reads in 195.725 CPU sec, 6.204 real sec [M::mem_process_seqs] Processed 700468 reads in 139.737 CPU sec, 4.415 real sec sorting block 77, #reads = 1082632 mem = 536877726 peak = 1053782016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 290008, 18, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 251, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011) [M::mem_pestat] mean and std.dev: (296.20, 195.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 227, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (256.28, 132.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 1075, 1880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4382) [M::mem_pestat] mean and std.dev: (1133.06, 719.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 327, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098) [M::mem_pestat] mean and std.dev: (326.51, 176.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1401) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707106 sequences (100000288 bp) in 3.447 CPU sec, 3.639 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (77, 291786, 15, 82) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 203, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 853) [M::mem_pestat] mean and std.dev: (239.51, 146.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (147, 217, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (245.00, 125.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (561, 912, 1863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4467) [M::mem_pestat] mean and std.dev: (987.54, 794.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5769) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 282, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (298.13, 171.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705794 sequences (100000006 bp) in 2.041 CPU sec, 2.046 real sec... [M::mem_process_seqs] Processed 704876 reads in 212.781 CPU sec, 6.745 real sec sorting block 78, #reads = 1076606 mem = 536885589 peak = 1053782016 [M::mem_process_seqs] Processed 709284 reads in 142.110 CPU sec, 4.498 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (87, 291023, 21, 71) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 328, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1562) [M::mem_pestat] mean and std.dev: (376.19, 278.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 222, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (250.32, 128.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (387, 860, 1655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4191) [M::mem_pestat] mean and std.dev: (1194.52, 1127.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5705) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 284, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030) [M::mem_pestat] mean and std.dev: (331.43, 212.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1310) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 705156 sequences (100000117 bp) in 3.055 CPU sec, 3.131 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 291445, 24, 69) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 368, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1485) [M::mem_pestat] mean and std.dev: (414.90, 323.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (152, 226, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (254.28, 130.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (175, 524, 2414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6892) [M::mem_pestat] mean and std.dev: (862.86, 1101.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9131) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 281, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1863) [M::mem_pestat] mean and std.dev: (509.35, 503.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2523) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 707106 reads in 188.024 CPU sec, 5.958 real sec sorting block 79, #reads = 1086183 mem = 536877377 peak = 1053782016 [M::process] read 704264 sequences (100000076 bp) in 2.737 CPU sec, 2.768 real sec... [M::mem_process_seqs] Processed 705794 reads in 132.822 CPU sec, 4.179 real sec sorting block 80, #reads = 1088297 mem = 536879831 peak = 1053782016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 290592, 25, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 271, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (294.92, 166.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 228, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (256.00, 131.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (444, 1004, 1759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4389) [M::mem_pestat] mean and std.dev: (1052.33, 895.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5704) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 305, 901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2347) [M::mem_pestat] mean and std.dev: (449.77, 411.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3070) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703294 sequences (100000048 bp) in 3.257 CPU sec, 3.424 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 289890, 20, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 303, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294) [M::mem_pestat] mean and std.dev: (296.39, 183.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 231, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (259.19, 133.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (196, 645, 4222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12274) [M::mem_pestat] mean and std.dev: (1673.90, 2072.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16300) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 293, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (304.27, 149.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1219) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 705156 reads in 182.151 CPU sec, 5.796 real sec [M::mem_process_seqs] Processed 704264 reads in 131.706 CPU sec, 4.144 real sec [M::process] read 702926 sequences (100000229 bp) in 3.185 CPU sec, 3.294 real sec... sorting block 81, #reads = 1088702 mem = 536881317 peak = 1053782016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (51, 289715, 24, 97) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 228, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113) [M::mem_pestat] mean and std.dev: (292.35, 218.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 233, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (261.70, 134.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (490, 916, 1899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4717) [M::mem_pestat] mean and std.dev: (1059.26, 857.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6126) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 246, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (265.03, 133.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702818 sequences (100000050 bp) in 3.305 CPU sec, 3.475 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 290025, 21, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 217, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042) [M::mem_pestat] mean and std.dev: (279.29, 191.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1334) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (155, 235, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (263.56, 136.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (220, 814, 3652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10516) [M::mem_pestat] mean and std.dev: (1722.05, 1983.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13948) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 366, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1476) [M::mem_pestat] mean and std.dev: (429.28, 290.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 703294 reads in 191.211 CPU sec, 6.114 real sec [M::mem_process_seqs] Processed 702926 reads in 135.974 CPU sec, 4.292 real sec sorting block 82, #reads = 1085337 mem = 536877748 peak = 1053782016 [M::process] read 702478 sequences (100000023 bp) in 3.326 CPU sec, 3.490 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 290145, 17, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 352, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2544) [M::mem_pestat] mean and std.dev: (429.27, 368.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 234, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (263.46, 136.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (784, 1352, 2335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5437) [M::mem_pestat] mean and std.dev: (1346.12, 828.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 288, 765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1933) [M::mem_pestat] mean and std.dev: (422.86, 324.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2517) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (79, 289400, 23, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 257, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948) [M::mem_pestat] mean and std.dev: (295.87, 201.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 237, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (265.30, 137.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (330, 664, 1780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4680) [M::mem_pestat] mean and std.dev: (1086.78, 1151.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6130) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 312, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1154) [M::mem_pestat] mean and std.dev: (325.75, 147.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1458) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701890 sequences (100000165 bp) in 3.524 CPU sec, 3.770 real sec... [M::mem_process_seqs] Processed 702818 reads in 200.161 CPU sec, 6.419 real sec sorting block 83, #reads = 1086303 mem = 536877487 peak = 1053782016 [M::mem_process_seqs] Processed 702478 reads in 141.607 CPU sec, 4.491 real sec [M::process] read 700976 sequences (100000132 bp) in 3.724 CPU sec, 3.983 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 289308, 24, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 293, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2038) [M::mem_pestat] mean and std.dev: (520.53, 458.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 239, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (268.02, 139.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (136, 580, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3463) [M::mem_pestat] mean and std.dev: (675.00, 740.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4572) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 263, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1268) [M::mem_pestat] mean and std.dev: (339.88, 232.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1629) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 84, #reads = 1084694 mem = 536879543 peak = 1053782016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 288963, 17, 67) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 262, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165) [M::mem_pestat] mean and std.dev: (280.37, 189.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (268.82, 138.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (268, 1164, 2223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6133) [M::mem_pestat] mean and std.dev: (841.93, 718.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8088) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 285, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (288.98, 143.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701890 reads in 195.013 CPU sec, 6.259 real sec [M::process] read 700668 sequences (100000048 bp) in 3.690 CPU sec, 3.930 real sec... [M::mem_process_seqs] Processed 700976 reads in 140.763 CPU sec, 4.470 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 288623, 24, 106) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 325, 944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2456) [M::mem_pestat] mean and std.dev: (493.58, 402.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3212) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 241, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (269.04, 138.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (285, 726, 1154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2892) [M::mem_pestat] mean and std.dev: (734.78, 573.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 347, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1236) [M::mem_pestat] mean and std.dev: (383.04, 241.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 85, #reads = 1084621 mem = 536883714 peak = 1053782016 [M::mem_process_seqs] Processed 700668 reads in 113.268 CPU sec, 3.721 real sec [M::process] read 699794 sequences (100000136 bp) in 4.102 CPU sec, 4.486 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (70, 289012, 31, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 386, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1909) [M::mem_pestat] mean and std.dev: (467.95, 404.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (269.87, 137.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (580, 761, 1818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4294) [M::mem_pestat] mean and std.dev: (1168.83, 842.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5532) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 288, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848) [M::mem_pestat] mean and std.dev: (287.82, 167.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699794 reads in 114.657 CPU sec, 3.763 real sec sorting block 86, #reads = 1083829 mem = 536878786 peak = 1053782016 [M::process] read 699758 sequences (100000134 bp) in 3.907 CPU sec, 4.231 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (84, 289172, 25, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 251, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (238.00, 111.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (270.46, 137.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (151, 544, 2090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5968) [M::mem_pestat] mean and std.dev: (1042.04, 1296.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7907) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 233, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (252.39, 132.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699758 reads in 113.590 CPU sec, 3.831 real sec [M::process] read 700210 sequences (100000020 bp) in 4.036 CPU sec, 4.412 real sec... sorting block 87, #reads = 1081798 mem = 536879017 peak = 1053782016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (75, 288927, 21, 92) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (144, 227, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1242) [M::mem_pestat] mean and std.dev: (275.20, 193.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (267.86, 136.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (355, 922, 1821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4753) [M::mem_pestat] mean and std.dev: (917.84, 760.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6219) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 270, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779) [M::mem_pestat] mean and std.dev: (284.62, 139.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 700160 sequences (100000244 bp) in 3.353 CPU sec, 3.560 real sec... [M::mem_process_seqs] Processed 700210 reads in 117.125 CPU sec, 3.770 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (52, 288274, 23, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (137, 298, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (249.49, 122.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (268.95, 137.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (657, 894, 1562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3372) [M::mem_pestat] mean and std.dev: (1155.57, 805.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4379) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 297, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1207) [M::mem_pestat] mean and std.dev: (341.75, 187.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 88, #reads = 1082311 mem = 536879177 peak = 1053782016 [M::mem_process_seqs] Processed 700160 reads in 117.104 CPU sec, 3.782 real sec [M::process] read 700166 sequences (100000081 bp) in 3.881 CPU sec, 4.186 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (93, 288247, 22, 74) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 239, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (265.13, 124.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (268.26, 137.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (150, 1545, 3166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9198) [M::mem_pestat] mean and std.dev: (1754.91, 1715.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 239, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (269.91, 160.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700166 reads in 119.343 CPU sec, 4.025 real sec [M::process] read 700128 sequences (100000105 bp) in 4.006 CPU sec, 4.315 real sec... sorting block 89, #reads = 1079978 mem = 536880960 peak = 1053782016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (78, 288174, 27, 94) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (160, 244, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (268.85, 167.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (268.39, 137.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (381, 732, 1830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4728) [M::mem_pestat] mean and std.dev: (941.30, 672.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6177) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 275, 578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1344) [M::mem_pestat] mean and std.dev: (343.55, 251.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700128 reads in 119.517 CPU sec, 3.857 real sec [M::process] read 700558 sequences (100000289 bp) in 3.799 CPU sec, 4.077 real sec... sorting block 90, #reads = 1080434 mem = 536880702 peak = 1053782016 [M::process] read 713668 sequences (100000084 bp) in 3.009 CPU sec, 3.096 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (66, 288276, 21, 103) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 295, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (377.14, 267.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 239, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (267.02, 137.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (424, 749, 1397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3343) [M::mem_pestat] mean and std.dev: (864.15, 665.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 235, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781) [M::mem_pestat] mean and std.dev: (270.54, 135.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 712536 sequences (100000023 bp) in 2.280 CPU sec, 2.280 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 293307, 19, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 231, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (272.42, 174.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (234.14, 119.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 794) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (387, 734, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2949) [M::mem_pestat] mean and std.dev: (896.84, 744.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3877) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 241, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949) [M::mem_pestat] mean and std.dev: (285.48, 188.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 700558 reads in 229.902 CPU sec, 7.224 real sec [M::process] read 709970 sequences (100000208 bp) in 2.212 CPU sec, 2.226 real sec... [M::mem_process_seqs] Processed 713668 reads in 159.190 CPU sec, 5.012 real sec sorting block 91, #reads = 1074803 mem = 536882405 peak = 1053782016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 292926, 15, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 322, 864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2230) [M::mem_pestat] mean and std.dev: (517.44, 484.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (143, 210, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (237.45, 120.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (192, 840, 2067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5817) [M::mem_pestat] mean and std.dev: (1174.00, 1270.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7692) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 321, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1885) [M::mem_pestat] mean and std.dev: (410.09, 342.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2440) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 707920 sequences (100000042 bp) in 3.106 CPU sec, 3.260 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (92, 291843, 22, 83) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (159, 273, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (250.13, 114.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (146, 217, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (244.47, 125.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (385, 596, 1908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4954) [M::mem_pestat] mean and std.dev: (1042.86, 898.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 307, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (321.01, 194.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1230) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 706270 sequences (100000094 bp) in 2.420 CPU sec, 2.421 real sec... [M::mem_process_seqs] Processed 712536 reads in 220.352 CPU sec, 6.998 real sec sorting block 92, #reads = 1083729 mem = 536878267 peak = 1053782016 [M::mem_process_seqs] Processed 709970 reads in 155.093 CPU sec, 4.922 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 291526, 19, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 331, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1192) [M::mem_pestat] mean and std.dev: (336.05, 212.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 221, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (249.50, 128.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (247, 825, 1746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4744) [M::mem_pestat] mean and std.dev: (943.58, 792.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6243) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 293, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922) [M::mem_pestat] mean and std.dev: (310.93, 196.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 704956 sequences (100000247 bp) in 3.150 CPU sec, 3.249 real sec... [M::process] read 703886 sequences (100000280 bp) in 2.240 CPU sec, 2.235 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 291103, 22, 66) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 230, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1088) [M::mem_pestat] mean and std.dev: (254.89, 161.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (151, 225, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (253.95, 130.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (539, 1191, 2034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5024) [M::mem_pestat] mean and std.dev: (1429.00, 1236.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6519) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 263, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1292) [M::mem_pestat] mean and std.dev: (345.87, 227.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1660) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 707920 reads in 206.048 CPU sec, 6.526 real sec sorting block 93, #reads = 1083206 mem = 536880221 peak = 1053782016 [M::mem_process_seqs] Processed 706270 reads in 242.394 CPU sec, 7.714 real sec sorting block 94, #reads = 1083312 mem = 536880256 peak = 1053782016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (64, 290320, 31, 92) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 231, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816) [M::mem_pestat] mean and std.dev: (243.85, 127.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (153, 229, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717) [M::mem_pestat] mean and std.dev: (257.40, 132.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (335, 818, 2437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6641) [M::mem_pestat] mean and std.dev: (1034.07, 917.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8743) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 295, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184) [M::mem_pestat] mean and std.dev: (357.56, 238.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1510) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 703158 sequences (100000245 bp) in 2.424 CPU sec, 2.559 real sec... [M::process] read 702906 sequences (100000034 bp) in 1.790 CPU sec, 1.794 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (67, 290006, 9, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 260, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (313.32, 210.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 232, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (260.64, 134.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 922) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 299, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1161) [M::mem_pestat] mean and std.dev: (352.85, 205.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1481) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 704956 reads in 198.557 CPU sec, 6.314 real sec [M::mem_process_seqs] Processed 703886 reads in 139.198 CPU sec, 4.440 real sec sorting block 95, #reads = 1083082 mem = 536880408 peak = 1053782016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (62, 289683, 16, 92) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 303, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (264.32, 130.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 235, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (264.47, 136.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (351, 452, 2570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7008) [M::mem_pestat] mean and std.dev: (985.40, 1048.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 277, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (298.16, 153.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1274) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702184 sequences (100000078 bp) in 3.267 CPU sec, 3.376 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (63, 289643, 26, 73) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 387, 1321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3543) [M::mem_pestat] mean and std.dev: (614.93, 629.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (156, 235, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (263.89, 136.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 936) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (275, 1732, 2303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6359) [M::mem_pestat] mean and std.dev: (1632.08, 1536.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8387) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 277, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (275.20, 121.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701908 sequences (100000152 bp) in 2.370 CPU sec, 2.373 real sec... [M::mem_process_seqs] Processed 702906 reads in 202.976 CPU sec, 6.418 real sec [M::mem_process_seqs] Processed 703158 reads in 232.415 CPU sec, 7.381 real sec sorting block 96, #reads = 1082309 mem = 536880642 peak = 1053782016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (65, 289151, 19, 76) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 304, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (288.96, 161.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 236, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (264.40, 136.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (299, 468, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2612) [M::mem_pestat] mean and std.dev: (468.00, 357.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3383) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 217, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (245.30, 121.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 702060 sequences (100000155 bp) in 3.351 CPU sec, 3.538 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (74, 289099, 26, 79) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 202, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (224.09, 115.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 237, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (265.25, 136.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (268, 621, 3232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9160) [M::mem_pestat] mean and std.dev: (1893.88, 2451.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12124) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 302, 721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1741) [M::mem_pestat] mean and std.dev: (422.07, 341.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2251) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702184 reads in 204.737 CPU sec, 6.485 real sec [M::process] read 701568 sequences (100000224 bp) in 2.829 CPU sec, 2.862 real sec... [M::mem_process_seqs] Processed 701908 reads in 145.808 CPU sec, 4.598 real sec sorting block 97, #reads = 1081471 mem = 536879105 peak = 1053782016 [M::process] read 700928 sequences (100000279 bp) in 3.255 CPU sec, 3.384 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (80, 288843, 36, 62) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 266, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (327.86, 232.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 236, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (263.95, 135.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (7, 580, 2322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6952) [M::mem_pestat] mean and std.dev: (1086.47, 1320.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9267) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 238, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (264.36, 142.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 701024 sequences (100000118 bp) in 2.450 CPU sec, 2.446 real sec... [M::mem_process_seqs] Processed 701568 reads in 116.233 CPU sec, 3.658 real sec sorting block 98, #reads = 1081368 mem = 536879254 peak = 1053782016 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 289178, 25, 86) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 314, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2293) [M::mem_pestat] mean and std.dev: (418.74, 370.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (157, 235, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (263.94, 135.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (432, 2669, 2669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7143) [M::mem_pestat] mean and std.dev: (1800.32, 1240.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 313, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (317.44, 144.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (97, 289121, 30, 84) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 214, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749) [M::mem_pestat] mean and std.dev: (239.14, 135.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (266.79, 137.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (146, 689, 1395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3893) [M::mem_pestat] mean and std.dev: (680.85, 598.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5142) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 294, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031) [M::mem_pestat] mean and std.dev: (335.67, 184.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 702060 reads in 305.460 CPU sec, 9.822 real sec [M::process] read 700824 sequences (100000020 bp) in 2.667 CPU sec, 2.719 real sec... [M::mem_process_seqs] Processed 700928 reads in 161.834 CPU sec, 5.142 real sec sorting block 99, #reads = 1083288 mem = 536878448 peak = 1118920704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (68, 289205, 38, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 231, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (241.46, 121.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (265.70, 135.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (458, 682, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3077) [M::mem_pestat] mean and std.dev: (961.56, 777.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4072) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 298, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1200) [M::mem_pestat] mean and std.dev: (358.55, 267.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1541) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 699980 sequences (100000300 bp) in 3.072 CPU sec, 3.205 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (69, 288689, 19, 94) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 295, 1096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2934) [M::mem_pestat] mean and std.dev: (528.00, 534.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 238, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (266.10, 136.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 931) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (319, 1247, 2586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7120) [M::mem_pestat] mean and std.dev: (1542.74, 1470.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9387) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 282, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1316) [M::mem_pestat] mean and std.dev: (340.77, 217.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1692) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 701024 reads in 188.204 CPU sec, 5.980 real sec sorting block 100, #reads = 1083188 mem = 536887759 peak = 1118920704 [M::process] read 699584 sequences (100000127 bp) in 2.452 CPU sec, 2.480 real sec... [M::mem_process_seqs] Processed 700824 reads in 134.309 CPU sec, 4.238 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (83, 288461, 16, 72) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 250, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (236.62, 88.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (160, 241, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (268.69, 137.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (354, 646, 1539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3909) [M::mem_pestat] mean and std.dev: (819.62, 603.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5094) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 319, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1474) [M::mem_pestat] mean and std.dev: (363.21, 230.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR sorting block 101, #reads = 1084129 mem = 536877537 peak = 1118920704 [M::process] read 440656 sequences (62869276 bp) in 2.313 CPU sec, 2.430 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (76, 288548, 12, 107) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 256, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007) [M::mem_pestat] mean and std.dev: (248.32, 137.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (161, 243, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 752) [M::mem_pestat] mean and std.dev: (270.78, 139.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (147, 553, 2214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6348) [M::mem_pestat] mean and std.dev: (664.20, 776.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 254, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991) [M::mem_pestat] mean and std.dev: (297.31, 190.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 699980 reads in 186.520 CPU sec, 6.012 real sec [M::mem_process_seqs] Processed 699584 reads in 131.911 CPU sec, 4.264 real sec sorting block 102, #reads = 1083047 mem = 536877005 peak = 1118920704 [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (42, 181444, 16, 70) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 185, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 755) [M::mem_pestat] mean and std.dev: (232.22, 136.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (159, 240, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750) [M::mem_pestat] mean and std.dev: (268.72, 138.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 608, 1468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3188) [M::mem_pestat] mean and std.dev: (858.27, 551.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4048) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 329, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1196) [M::mem_pestat] mean and std.dev: (345.48, 193.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 440656 reads in 72.967 CPU sec, 2.437 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: INTEL(R) XEON(R) PLATINUM 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 111966800 overall: 70046633984 mem 19309.356 user 515.823 sys 659.128 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1136_6TL-T1-TDNA-1_A23MHWWLT4_1.L003\tPU:L003\tSM:1136_6TL-T1-TDNA-1_A23MHWWLT4_1\tLB:1136_6TL-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 1136_6TL-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 1136_6TL-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz [main] Real time: 659.178 sec; CPU: 19825.191 sec sorting block 103, #reads = 1003632 mem = 498065236 peak = 1118920704 uniq header count 1 merging 104 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: INTEL(R) XEON(R) PLATINUM 8559C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 6043587825 output reads: 112732195 bam_mem_sort: 104 calls 66.671 user 1.682 sys 68.574 real bam_write: 104 calls 53.652 user 3.004 sys 62.601 real execute: 1 calls 39.568 user 1.979 sys 708.528 real merge_files: 1 calls 39.528 user 1.955 sys 48.537 real parse_chunk: 1663 calls 100.024 user 22.226 sys 126.582 real read_chunk: 4496 calls 59.614 user 28.916 sys 610.394 real sort_block: 1 calls 39.808 user 28.272 sys 585.167 real write_chunk: 2831 calls 39.145 user 1.596 sys 47.513 real overall: 3261321216 mem 1915.732 user 74.798 sys 708.556 real ls: cannot access '*.cram': No such file or directory