cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1577.115 CPU sec, 532.326 real sec
[M::process] read 698304 sequences (100000298 bp) in 0.950 CPU sec, 0.954 real sec...
[M::process] read 696658 sequences (100000288 bp) in 1.219 CPU sec, 1.246 real sec...
[M::process] read 698858 sequences (100000036 bp) in 1.182 CPU sec, 1.192 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290701, 4, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 211, 222)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (84, 314)
[M::mem_pestat] mean and std.dev: (191.92, 41.35)
[M::mem_pestat] low and high boundaries for proper pairs: (27, 360)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.49, 122.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699242 sequences (100000021 bp) in 1.143 CPU sec, 1.163 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290234, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 235, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (260.12, 125.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 166, 1009)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2723)
[M::mem_pestat] mean and std.dev: (495.60, 445.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3580)
[M::mem_pestat] skip orientation RR
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291236, 11, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.81, 122.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (752, 752, 809)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (638, 923)
[M::mem_pestat] mean and std.dev: (780.11, 56.32)
[M::mem_pestat] low and high boundaries for proper pairs: (555, 1005)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 698304 reads in 208.264 CPU sec, 6.672 real sec
[M::mem_process_seqs] Processed 696658 reads in 136.289 CPU sec, 4.391 real sec
[M::mem_process_seqs] Processed 698858 reads in 147.496 CPU sec, 4.766 real sec
sorting block 0, #reads = 1093135 mem = 536879950 peak = 593489920
[M::process] read 698470 sequences (100000295 bp) in 1.582 CPU sec, 1.696 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290918, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.59, 120.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696582 sequences (100000121 bp) in 1.235 CPU sec, 1.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291504, 5, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (253.38, 123.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699074 sequences (100000067 bp) in 1.120 CPU sec, 1.121 real sec...
[M::mem_process_seqs] Processed 699242 reads in 127.076 CPU sec, 4.032 real sec
sorting block 1, #reads = 1092909 mem = 536875972 peak = 800169984
[M::mem_process_seqs] Processed 698470 reads in 79.567 CPU sec, 2.516 real sec
sorting block 2, #reads = 1094764 mem = 536883418 peak = 887259136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290500, 9, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 340)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (259.57, 126.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 877)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 307, 414)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (307.92, 160.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1065)
[M::mem_pestat] skip orientation RR
[M::process] read 698358 sequences (100000143 bp) in 1.696 CPU sec, 1.787 real sec...
[M::process] read 697214 sequences (100000050 bp) in 1.232 CPU sec, 1.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291362, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 228, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (225.55, 74.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (251.00, 121.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696582 reads in 126.163 CPU sec, 4.015 real sec
[M::mem_process_seqs] Processed 699074 reads in 78.828 CPU sec, 2.516 real sec
sorting block 3, #reads = 1093832 mem = 536879078 peak = 887259136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290938, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (252.86, 122.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696172 sequences (100000143 bp) in 1.621 CPU sec, 1.700 real sec...
[M::process] read 697172 sequences (100000070 bp) in 1.200 CPU sec, 1.203 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290631, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.33, 121.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698358 reads in 127.192 CPU sec, 4.054 real sec
[M::mem_process_seqs] Processed 697214 reads in 79.339 CPU sec, 2.533 real sec
sorting block 4, #reads = 1095014 mem = 536879377 peak = 887259136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289859, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (256.65, 122.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696238 sequences (100000072 bp) in 1.624 CPU sec, 1.720 real sec...
[M::process] read 696322 sequences (100000054 bp) in 1.240 CPU sec, 1.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290505, 4, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.99, 120.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 249, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (253.82, 68.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1205)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696172 reads in 128.251 CPU sec, 4.095 real sec
sorting block 5, #reads = 1093371 mem = 536887243 peak = 887259136
[M::mem_process_seqs] Processed 697172 reads in 79.850 CPU sec, 2.536 real sec
sorting block 6, #reads = 1093597 mem = 536885208 peak = 887259136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 289993, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (254.29, 120.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (16, 140, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1327)
[M::mem_pestat] mean and std.dev: (261.80, 277.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1764)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 694564 sequences (100000100 bp) in 1.979 CPU sec, 2.088 real sec...
[M::process] read 696640 sequences (100000252 bp) in 1.270 CPU sec, 1.271 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290094, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (252.59, 119.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 349, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745)
[M::mem_pestat] mean and std.dev: (309.30, 113.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 931)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696238 reads in 128.851 CPU sec, 4.104 real sec
[M::mem_process_seqs] Processed 696322 reads in 80.193 CPU sec, 2.547 real sec
sorting block 7, #reads = 1092341 mem = 536878063 peak = 887259136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 289749, 6, 13)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (178, 286, 740)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1864)
[M::mem_pestat] mean and std.dev: (319.22, 234.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2426)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 237, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (259.68, 122.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 860)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 324, 950)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2342)
[M::mem_pestat] mean and std.dev: (496.83, 323.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3038)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 695422 sequences (100000023 bp) in 1.800 CPU sec, 1.922 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290403, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (251.83, 118.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696334 sequences (100000221 bp) in 1.310 CPU sec, 1.315 real sec...
[M::mem_process_seqs] Processed 694564 reads in 127.819 CPU sec, 4.081 real sec
[M::mem_process_seqs] Processed 696640 reads in 79.925 CPU sec, 2.535 real sec
sorting block 8, #reads = 1092414 mem = 536878039 peak = 887259136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 289987, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 310, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184)
[M::mem_pestat] mean and std.dev: (298.09, 207.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1525)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.91, 120.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 695970 sequences (100000006 bp) in 2.108 CPU sec, 2.220 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290522, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (253.70, 120.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698056 sequences (100000223 bp) in 1.330 CPU sec, 1.330 real sec...
[M::mem_process_seqs] Processed 695422 reads in 129.007 CPU sec, 4.110 real sec
[M::mem_process_seqs] Processed 696334 reads in 80.735 CPU sec, 2.564 real sec
sorting block 9, #reads = 1093172 mem = 536879014 peak = 887259136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 289813, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (255.94, 122.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697986 sequences (100000223 bp) in 1.956 CPU sec, 2.073 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290465, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 232, 1088)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2902)
[M::mem_pestat] mean and std.dev: (441.50, 423.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3809)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.14, 122.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698074 sequences (100000094 bp) in 1.340 CPU sec, 1.343 real sec...
[M::mem_process_seqs] Processed 695970 reads in 130.401 CPU sec, 4.166 real sec
sorting block 10, #reads = 1092438 mem = 536878431 peak = 887259136
[M::mem_process_seqs] Processed 698056 reads in 81.861 CPU sec, 2.602 real sec
sorting block 11, #reads = 1092570 mem = 536878693 peak = 900292608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290827, 7, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 230, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593)
[M::mem_pestat] mean and std.dev: (270.20, 123.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.39, 121.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698178 sequences (100000252 bp) in 2.093 CPU sec, 2.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290807, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687)
[M::mem_pestat] mean and std.dev: (255.59, 123.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 863)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698582 sequences (100000048 bp) in 1.350 CPU sec, 1.347 real sec...
[M::mem_process_seqs] Processed 697986 reads in 128.452 CPU sec, 4.096 real sec
[M::mem_process_seqs] Processed 698074 reads in 80.399 CPU sec, 2.555 real sec
sorting block 12, #reads = 1094134 mem = 536879695 peak = 900292608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291077, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (254.62, 123.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696772 sequences (100000061 bp) in 1.919 CPU sec, 2.047 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290829, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.87, 121.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698178 reads in 126.934 CPU sec, 4.057 real sec
[M::process] read 698130 sequences (100000164 bp) in 1.360 CPU sec, 1.365 real sec...
[M::mem_process_seqs] Processed 698582 reads in 80.426 CPU sec, 2.548 real sec
sorting block 13, #reads = 1094489 mem = 536880275 peak = 900292608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290508, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689)
[M::mem_pestat] mean and std.dev: (257.99, 124.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 865)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 237, 1229)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3335)
[M::mem_pestat] mean and std.dev: (477.18, 470.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4388)
[M::mem_pestat] skip orientation RR
[M::process] read 697890 sequences (100000196 bp) in 2.506 CPU sec, 2.648 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291171, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.03, 121.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (379, 843, 843)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1771)
[M::mem_pestat] mean and std.dev: (559.50, 255.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2235)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696772 reads in 127.489 CPU sec, 4.070 real sec
[M::mem_process_seqs] Processed 698130 reads in 80.518 CPU sec, 2.546 real sec
sorting block 14, #reads = 1094327 mem = 536881075 peak = 900292608
[M::process] read 697322 sequences (100000180 bp) in 1.704 CPU sec, 1.761 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291066, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.20, 122.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 15, #reads = 1094397 mem = 536880495 peak = 900292608
[M::process] read 694908 sequences (100000195 bp) in 1.807 CPU sec, 1.922 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290890, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.43, 122.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697890 reads in 127.261 CPU sec, 4.074 real sec
[M::process] read 696676 sequences (100000207 bp) in 1.382 CPU sec, 1.396 real sec...
[M::mem_process_seqs] Processed 697322 reads in 80.135 CPU sec, 2.539 real sec
sorting block 16, #reads = 1094733 mem = 536879861 peak = 900292608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290021, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 238, 342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 698)
[M::mem_pestat] mean and std.dev: (261.80, 125.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 876)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696830 sequences (100000207 bp) in 2.203 CPU sec, 2.325 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290943, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.10, 120.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 694908 reads in 127.558 CPU sec, 4.068 real sec
[M::process] read 697144 sequences (100000102 bp) in 1.425 CPU sec, 1.434 real sec...
[M::mem_process_seqs] Processed 696676 reads in 80.750 CPU sec, 2.563 real sec
sorting block 17, #reads = 1093077 mem = 536877074 peak = 900292608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290449, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.76, 121.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695926 sequences (100000064 bp) in 1.855 CPU sec, 1.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290595, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (251.87, 120.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695834 sequences (100000284 bp) in 1.340 CPU sec, 1.341 real sec...
[M::mem_process_seqs] Processed 696830 reads in 128.765 CPU sec, 4.116 real sec
[M::mem_process_seqs] Processed 697144 reads in 80.434 CPU sec, 2.554 real sec
sorting block 18, #reads = 1093746 mem = 536879825 peak = 900292608
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290472, 11, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (254.57, 120.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (408, 607, 3624)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10056)
[M::mem_pestat] mean and std.dev: (1471.91, 1635.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 13272)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 216, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (36, 361)
[M::mem_pestat] mean and std.dev: (192.20, 45.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 426)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696788 sequences (100000194 bp) in 2.183 CPU sec, 2.311 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290270, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.36, 120.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 226, 419)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (277.56, 148.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RR
[M::process] read 696790 sequences (100000186 bp) in 1.360 CPU sec, 1.355 real sec...
[M::mem_process_seqs] Processed 695926 reads in 128.601 CPU sec, 4.095 real sec
sorting block 19, #reads = 1093692 mem = 536880927 peak = 900292608
[M::mem_process_seqs] Processed 695834 reads in 80.800 CPU sec, 2.561 real sec
sorting block 20, #reads = 1092423 mem = 536878559 peak = 942546944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290353, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (252.03, 119.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697180 sequences (100000124 bp) in 1.815 CPU sec, 1.928 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 290242, 7, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 1360, 1926)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5412)
[M::mem_pestat] mean and std.dev: (1075.27, 832.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7155)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.21, 119.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697624 sequences (100000172 bp) in 1.370 CPU sec, 1.366 real sec...
[M::mem_process_seqs] Processed 696788 reads in 123.898 CPU sec, 3.941 real sec
[M::mem_process_seqs] Processed 696790 reads in 78.114 CPU sec, 2.469 real sec
sorting block 21, #reads = 1092649 mem = 536879795 peak = 942546944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290197, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.59, 120.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699110 sequences (100000008 bp) in 1.945 CPU sec, 2.045 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290444, 10, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.50, 120.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (263, 849, 1700)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4574)
[M::mem_pestat] mean and std.dev: (858.20, 754.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6011)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 698986 sequences (100000021 bp) in 1.350 CPU sec, 1.347 real sec...
[M::mem_process_seqs] Processed 697180 reads in 126.793 CPU sec, 4.034 real sec
[M::mem_process_seqs] Processed 697624 reads in 81.166 CPU sec, 2.567 real sec
sorting block 22, #reads = 1092232 mem = 536880238 peak = 942546944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291239, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.12, 118.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697964 sequences (100000010 bp) in 1.900 CPU sec, 2.013 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290548, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.68, 119.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (405, 405, 702)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1296)
[M::mem_pestat] mean and std.dev: (488.60, 247.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1593)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 696712 sequences (100000213 bp) in 1.340 CPU sec, 1.346 real sec...
[M::mem_process_seqs] Processed 699110 reads in 127.863 CPU sec, 4.074 real sec
[M::mem_process_seqs] Processed 698986 reads in 81.261 CPU sec, 2.575 real sec
sorting block 23, #reads = 1091860 mem = 536879296 peak = 942546944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 291140, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (235, 254, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072)
[M::mem_pestat] mean and std.dev: (296.08, 116.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1351)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.61, 120.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697082 sequences (100000250 bp) in 1.919 CPU sec, 2.029 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290431, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 236, 341)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699)
[M::mem_pestat] mean and std.dev: (260.40, 125.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 878)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698382 sequences (100000044 bp) in 1.340 CPU sec, 1.335 real sec...
[M::mem_process_seqs] Processed 697964 reads in 126.461 CPU sec, 4.028 real sec
sorting block 24, #reads = 1092356 mem = 536877954 peak = 942546944
[M::mem_process_seqs] Processed 696712 reads in 80.058 CPU sec, 2.530 real sec
sorting block 25, #reads = 1093533 mem = 536879353 peak = 942546944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290358, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (256.63, 122.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 857)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697964 sequences (100000163 bp) in 1.905 CPU sec, 2.004 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291099, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 158, 248)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440)
[M::mem_pestat] mean and std.dev: (169.62, 32.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 536)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.44, 121.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697082 reads in 124.977 CPU sec, 3.982 real sec
[M::process] read 697986 sequences (100000115 bp) in 1.378 CPU sec, 1.381 real sec...
[M::mem_process_seqs] Processed 698382 reads in 79.719 CPU sec, 2.518 real sec
sorting block 26, #reads = 1092810 mem = 536880933 peak = 942546944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290837, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 315, 575)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1359)
[M::mem_pestat] mean and std.dev: (408.83, 325.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1751)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.71, 121.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697416 sequences (100000194 bp) in 1.971 CPU sec, 2.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290749, 4, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 273, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (227.50, 80.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (253.96, 121.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 197, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1190)
[M::mem_pestat] mean and std.dev: (342.10, 232.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1532)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697964 reads in 125.718 CPU sec, 4.009 real sec
[M::process] read 694734 sequences (100000219 bp) in 1.399 CPU sec, 1.410 real sec...
[M::mem_process_seqs] Processed 697986 reads in 80.803 CPU sec, 2.552 real sec
sorting block 27, #reads = 1093662 mem = 536879919 peak = 942546944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290812, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 232, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (255.65, 122.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 856)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697006 sequences (100000228 bp) in 1.911 CPU sec, 2.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290009, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (164, 240, 343)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701)
[M::mem_pestat] mean and std.dev: (262.67, 125.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 880)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697416 reads in 124.316 CPU sec, 3.959 real sec
[M::process] read 696798 sequences (100000018 bp) in 1.368 CPU sec, 1.377 real sec...
sorting block 28, #reads = 1093955 mem = 536880062 peak = 942546944
[M::mem_process_seqs] Processed 694734 reads in 78.742 CPU sec, 2.489 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290892, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.32, 121.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 257, 1154)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3094)
[M::mem_pestat] mean and std.dev: (434.75, 422.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4064)
[M::mem_pestat] skip orientation RR
sorting block 29, #reads = 1092435 mem = 536879248 peak = 980639744
[M::process] read 696876 sequences (100000146 bp) in 2.118 CPU sec, 2.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290178, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.05, 121.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697006 reads in 125.893 CPU sec, 4.005 real sec
[M::process] read 697008 sequences (100000142 bp) in 1.392 CPU sec, 1.397 real sec...
[M::mem_process_seqs] Processed 696798 reads in 80.068 CPU sec, 2.532 real sec
sorting block 30, #reads = 1093639 mem = 536880071 peak = 980639744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290534, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.43, 119.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697058 sequences (100000070 bp) in 1.874 CPU sec, 1.982 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290443, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.38, 119.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696876 reads in 125.179 CPU sec, 3.991 real sec
[M::process] read 696672 sequences (100000271 bp) in 1.377 CPU sec, 1.374 real sec...
[M::mem_process_seqs] Processed 697008 reads in 79.727 CPU sec, 2.518 real sec
sorting block 31, #reads = 1092857 mem = 536879826 peak = 980639744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290226, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.10, 118.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696886 sequences (100000157 bp) in 2.233 CPU sec, 2.340 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290023, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.24, 119.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697058 reads in 125.384 CPU sec, 3.985 real sec
[M::process] read 696852 sequences (100000240 bp) in 1.408 CPU sec, 1.422 real sec...
[M::mem_process_seqs] Processed 696672 reads in 79.492 CPU sec, 2.509 real sec
sorting block 32, #reads = 1093613 mem = 536879357 peak = 980639744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290687, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.47, 120.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697768 sequences (100000284 bp) in 1.862 CPU sec, 1.971 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289588, 7, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.56, 119.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 245, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 722)
[M::mem_pestat] mean and std.dev: (213.73, 71.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 903)
[M::mem_pestat] skip orientation RR
[M::process] read 695940 sequences (100000183 bp) in 1.340 CPU sec, 1.341 real sec...
[M::mem_process_seqs] Processed 696886 reads in 126.310 CPU sec, 4.022 real sec
sorting block 33, #reads = 1092617 mem = 536878253 peak = 980639744
[M::mem_process_seqs] Processed 696852 reads in 81.171 CPU sec, 2.569 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290393, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.61, 119.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 34, #reads = 1093720 mem = 536878863 peak = 1026232320
[M::process] read 696230 sequences (100000298 bp) in 2.412 CPU sec, 2.561 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 290328, 13, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (328, 328, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (379.47, 236.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1327)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 235, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (257.07, 121.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (507, 570, 3052)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8142)
[M::mem_pestat] mean and std.dev: (1268.46, 1201.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10687)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 281, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 538)
[M::mem_pestat] mean and std.dev: (225.60, 79.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 658)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697768 reads in 125.208 CPU sec, 3.985 real sec
[M::mem_process_seqs] Processed 695940 reads in 79.487 CPU sec, 2.510 real sec
[M::process] read 697292 sequences (100000028 bp) in 1.521 CPU sec, 1.562 real sec...
sorting block 35, #reads = 1092900 mem = 536883279 peak = 1026232320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290086, 2, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (165, 192, 2424)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6942)
[M::mem_pestat] mean and std.dev: (1252.92, 1901.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9201)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 234, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (255.92, 120.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 694636 sequences (100000256 bp) in 1.861 CPU sec, 2.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291111, 1, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 419, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1208)
[M::mem_pestat] mean and std.dev: (319.56, 171.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1555)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.42, 119.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 452, 1176)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3184)
[M::mem_pestat] mean and std.dev: (928.60, 1091.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5295)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696230 reads in 122.836 CPU sec, 3.926 real sec
[M::mem_process_seqs] Processed 697292 reads in 78.390 CPU sec, 2.473 real sec
[M::process] read 697768 sequences (100000170 bp) in 1.553 CPU sec, 1.585 real sec...
sorting block 36, #reads = 1094187 mem = 536880926 peak = 1026232320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 289383, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (104, 226, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (199.27, 116.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 241, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705)
[M::mem_pestat] mean and std.dev: (263.89, 125.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 885)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698608 sequences (100000081 bp) in 2.221 CPU sec, 2.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290526, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (254.60, 122.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 694636 reads in 123.956 CPU sec, 3.951 real sec
[M::mem_process_seqs] Processed 697768 reads in 79.770 CPU sec, 2.523 real sec
sorting block 37, #reads = 1093343 mem = 536882403 peak = 1026232320
[M::process] read 698434 sequences (100000254 bp) in 1.606 CPU sec, 1.666 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290786, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.02, 120.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1092658 mem = 536879481 peak = 1026232320
[M::process] read 698546 sequences (100000153 bp) in 1.808 CPU sec, 1.918 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291316, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.86, 120.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698608 reads in 127.319 CPU sec, 4.065 real sec
[M::process] read 696876 sequences (100000139 bp) in 1.407 CPU sec, 1.415 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291499, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.55, 121.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696430 sequences (100000268 bp) in 1.520 CPU sec, 1.529 real sec...
[M::mem_process_seqs] Processed 698546 reads in 72.770 CPU sec, 2.464 real sec
sorting block 39, #reads = 1094551 mem = 536879953 peak = 1026232320
[M::mem_process_seqs] Processed 698434 reads in 178.591 CPU sec, 5.826 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290182, 3, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 293, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (40, 470)
[M::mem_pestat] mean and std.dev: (257.08, 55.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 233, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 683)
[M::mem_pestat] mean and std.dev: (256.78, 123.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 857)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (119, 275, 281)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605)
[M::mem_pestat] mean and std.dev: (221.82, 95.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 767)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 40, #reads = 1094828 mem = 536880806 peak = 1026232320
[M::process] read 697110 sequences (100000038 bp) in 1.895 CPU sec, 2.016 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290552, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (161, 211, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1166)
[M::mem_pestat] mean and std.dev: (278.73, 259.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1501)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 234, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686)
[M::mem_pestat] mean and std.dev: (257.41, 123.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 861)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696876 reads in 137.938 CPU sec, 4.389 real sec
[M::mem_process_seqs] Processed 696430 reads in 81.046 CPU sec, 2.561 real sec
[M::process] read 696192 sequences (100000121 bp) in 1.548 CPU sec, 1.606 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291202, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 231, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.55, 121.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1094080 mem = 536877624 peak = 1026232320
[M::process] read 696690 sequences (100000252 bp) in 2.207 CPU sec, 2.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 289976, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 235, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687)
[M::mem_pestat] mean and std.dev: (258.29, 123.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 862)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697110 reads in 126.651 CPU sec, 4.027 real sec
[M::process] read 696978 sequences (100000259 bp) in 1.461 CPU sec, 1.480 real sec...
[M::mem_process_seqs] Processed 696192 reads in 81.177 CPU sec, 2.566 real sec
sorting block 42, #reads = 1095099 mem = 536877342 peak = 1026232320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290465, 2, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 169, 232)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (16, 376)
[M::mem_pestat] mean and std.dev: (174.80, 37.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 448)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.97, 121.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697256 sequences (100000220 bp) in 1.882 CPU sec, 2.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290513, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (176, 183, 257)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 419)
[M::mem_pestat] mean and std.dev: (209.22, 71.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 500)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.43, 118.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696690 reads in 128.260 CPU sec, 4.105 real sec
[M::process] read 696968 sequences (100000139 bp) in 1.432 CPU sec, 1.449 real sec...
[M::mem_process_seqs] Processed 696978 reads in 81.675 CPU sec, 2.591 real sec
sorting block 43, #reads = 1093131 mem = 536878822 peak = 1026232320
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290602, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (250.98, 118.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696656 sequences (100000015 bp) in 2.095 CPU sec, 2.207 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290387, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.72, 119.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699974 sequences (100000017 bp) in 1.360 CPU sec, 1.361 real sec...
[M::mem_process_seqs] Processed 697256 reads in 128.628 CPU sec, 4.090 real sec
sorting block 44, #reads = 1091628 mem = 536878788 peak = 1026232320
[M::mem_process_seqs] Processed 696968 reads in 81.440 CPU sec, 2.580 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290265, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.36, 119.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 45, #reads = 1091967 mem = 536877805 peak = 1034252288
[M::process] read 702032 sequences (100000274 bp) in 2.112 CPU sec, 2.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290875, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (244.07, 116.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696656 reads in 131.472 CPU sec, 4.182 real sec
[M::process] read 701804 sequences (100000161 bp) in 1.419 CPU sec, 1.416 real sec...
[M::mem_process_seqs] Processed 699974 reads in 85.322 CPU sec, 2.700 real sec
sorting block 46, #reads = 1092590 mem = 536882722 peak = 1034252288
[M::process] read 702862 sequences (100000215 bp) in 2.234 CPU sec, 2.351 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291826, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (254, 430, 598)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1286)
[M::mem_pestat] mean and std.dev: (349.80, 129.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1630)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (244.58, 120.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702312 sequences (100000183 bp) in 1.380 CPU sec, 1.380 real sec...
[M::mem_process_seqs] Processed 701804 reads in 73.675 CPU sec, 2.313 real sec
sorting block 47, #reads = 1091742 mem = 536877445 peak = 1034252288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292312, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (244.36, 120.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292009, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (241.24, 117.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702032 reads in 207.182 CPU sec, 6.574 real sec
[M::process] read 699296 sequences (100000093 bp) in 1.725 CPU sec, 1.729 real sec...
[M::mem_process_seqs] Processed 702862 reads in 97.191 CPU sec, 3.093 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 292490, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (241.33, 116.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 48, #reads = 1094422 mem = 536877945 peak = 1034252288
[M::process] read 702632 sequences (100000174 bp) in 2.176 CPU sec, 2.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291767, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (251.58, 123.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702312 reads in 132.772 CPU sec, 4.244 real sec
[M::mem_process_seqs] Processed 699296 reads in 84.695 CPU sec, 2.673 real sec
[M::process] read 701468 sequences (100000175 bp) in 1.561 CPU sec, 1.601 real sec...
sorting block 49, #reads = 1094815 mem = 536877911 peak = 1034252288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292549, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.58, 116.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (141, 261, 261)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501)
[M::mem_pestat] mean and std.dev: (201.78, 62.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 621)
[M::mem_pestat] skip orientation RR
[M::process] read 699068 sequences (100000247 bp) in 2.025 CPU sec, 2.147 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 292062, 3, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 348, 588)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1316)
[M::mem_pestat] mean and std.dev: (436.57, 288.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1680)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (244.66, 119.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (129, 181, 1531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4335)
[M::mem_pestat] mean and std.dev: (723.30, 961.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5737)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698874 sequences (100000227 bp) in 1.350 CPU sec, 1.349 real sec...
[M::mem_process_seqs] Processed 702632 reads in 133.845 CPU sec, 4.259 real sec
[M::mem_process_seqs] Processed 701468 reads in 84.771 CPU sec, 2.690 real sec
sorting block 50, #reads = 1093113 mem = 536878697 peak = 1034252288
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291539, 5, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (252.24, 123.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (1039, 1144, 1189)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (739, 1489)
[M::mem_pestat] mean and std.dev: (1116.22, 68.48)
[M::mem_pestat] low and high boundaries for proper pairs: (589, 1639)
[M::mem_pestat] skip orientation RR
[M::process] read 698022 sequences (100000155 bp) in 2.091 CPU sec, 2.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291048, 3, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (217, 425, 1191)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3139)
[M::mem_pestat] mean and std.dev: (648.91, 630.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4113)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (252.75, 123.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 849)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699068 reads in 132.509 CPU sec, 4.221 real sec
[M::process] read 697114 sequences (100000128 bp) in 1.407 CPU sec, 1.410 real sec...
sorting block 51, #reads = 1093001 mem = 536880708 peak = 1034252288
[M::mem_process_seqs] Processed 698874 reads in 84.681 CPU sec, 2.681 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291051, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (181, 287, 692)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1714)
[M::mem_pestat] mean and std.dev: (370.92, 232.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2225)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (253.66, 123.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 52, #reads = 1093320 mem = 536878809 peak = 1092456448
[M::process] read 697086 sequences (100000140 bp) in 2.023 CPU sec, 2.155 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290263, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.19, 120.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698022 reads in 132.378 CPU sec, 4.220 real sec
[M::process] read 697274 sequences (100000050 bp) in 1.427 CPU sec, 1.424 real sec...
[M::mem_process_seqs] Processed 697114 reads in 85.133 CPU sec, 2.696 real sec
sorting block 53, #reads = 1090878 mem = 536880252 peak = 1092456448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291083, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (254, 365, 608)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1316)
[M::mem_pestat] mean and std.dev: (307.67, 143.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1670)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (255.35, 123.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697440 sequences (100000056 bp) in 2.058 CPU sec, 2.190 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290111, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (336, 381, 417)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (174, 579)
[M::mem_pestat] mean and std.dev: (388.00, 24.66)
[M::mem_pestat] low and high boundaries for proper pairs: (93, 660)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (253.09, 122.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697086 reads in 133.133 CPU sec, 4.239 real sec
[M::process] read 697766 sequences (100000097 bp) in 1.380 CPU sec, 1.380 real sec...
[M::mem_process_seqs] Processed 697274 reads in 85.177 CPU sec, 2.698 real sec
sorting block 54, #reads = 1090371 mem = 536888990 peak = 1092456448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290783, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.82, 120.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697416 sequences (100000185 bp) in 2.217 CPU sec, 2.324 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290802, 9, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.64, 121.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703078 sequences (100000114 bp) in 1.430 CPU sec, 1.429 real sec...
[M::mem_process_seqs] Processed 697440 reads in 132.139 CPU sec, 4.197 real sec
[M::mem_process_seqs] Processed 697766 reads in 83.953 CPU sec, 2.653 real sec
sorting block 55, #reads = 1090001 mem = 536879385 peak = 1092456448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290486, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.29, 121.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1090927 mem = 536884500 peak = 1092456448
[M::process] read 704626 sequences (100000070 bp) in 1.997 CPU sec, 2.083 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292508, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.01, 116.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 345, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (261.91, 70.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RR
[M::process] read 703426 sequences (100000012 bp) in 1.420 CPU sec, 1.419 real sec...
[M::mem_process_seqs] Processed 697416 reads in 138.764 CPU sec, 4.421 real sec
[M::process] read 702976 sequences (100000285 bp) in 1.846 CPU sec, 1.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293093, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (238.07, 115.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704626 reads in 82.908 CPU sec, 2.624 real sec
sorting block 57, #reads = 1088967 mem = 536884145 peak = 1092456448
[M::mem_process_seqs] Processed 703078 reads in 207.560 CPU sec, 6.593 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 292553, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (239.67, 115.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1088375 mem = 536880448 peak = 1092456448
[M::process] read 701566 sequences (100000034 bp) in 2.169 CPU sec, 2.309 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292584, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.72, 115.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703426 reads in 158.537 CPU sec, 5.041 real sec
[M::mem_process_seqs] Processed 702976 reads in 91.908 CPU sec, 2.900 real sec
[M::process] read 702218 sequences (100000189 bp) in 1.576 CPU sec, 1.608 real sec...
sorting block 59, #reads = 1089102 mem = 536880163 peak = 1092456448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292658, 8, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.09, 117.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 211, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604)
[M::mem_pestat] mean and std.dev: (241.00, 81.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation RR
[M::process] read 702624 sequences (100000168 bp) in 2.217 CPU sec, 2.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292198, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.82, 116.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 228, 428)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872)
[M::mem_pestat] mean and std.dev: (259.88, 99.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1094)
[M::mem_pestat] skip orientation RR
[M::process] read 700910 sequences (100000133 bp) in 1.410 CPU sec, 1.408 real sec...
[M::mem_process_seqs] Processed 701566 reads in 140.458 CPU sec, 4.468 real sec
[M::mem_process_seqs] Processed 702218 reads in 88.534 CPU sec, 2.809 real sec
sorting block 60, #reads = 1090566 mem = 536876762 peak = 1092456448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292824, 9, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (167, 216, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (25, 380)
[M::mem_pestat] mean and std.dev: (195.36, 46.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 451)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.89, 115.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (132, 155, 224)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408)
[M::mem_pestat] mean and std.dev: (166.11, 45.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 500)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698816 sequences (100000060 bp) in 2.123 CPU sec, 2.270 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292452, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.63, 118.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702624 reads in 139.370 CPU sec, 4.442 real sec
[M::process] read 698908 sequences (100000233 bp) in 1.390 CPU sec, 1.386 real sec...
[M::mem_process_seqs] Processed 700910 reads in 88.545 CPU sec, 2.801 real sec
sorting block 61, #reads = 1091405 mem = 536879971 peak = 1092456448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291352, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (211, 274, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126)
[M::mem_pestat] mean and std.dev: (420.73, 340.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1782)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (253.47, 124.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698288 sequences (100000007 bp) in 2.262 CPU sec, 2.360 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291388, 3, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.84, 122.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 263, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830)
[M::mem_pestat] mean and std.dev: (286.12, 104.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RR
[M::process] read 697644 sequences (100000087 bp) in 1.370 CPU sec, 1.375 real sec...
[M::mem_process_seqs] Processed 698816 reads in 135.983 CPU sec, 4.322 real sec
sorting block 62, #reads = 1091213 mem = 536878904 peak = 1092456448
[M::mem_process_seqs] Processed 698908 reads in 86.254 CPU sec, 2.733 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291096, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.87, 121.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 215, 780)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1978)
[M::mem_pestat] mean and std.dev: (364.91, 302.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2577)
[M::mem_pestat] skip orientation RR
sorting block 63, #reads = 1090150 mem = 536879275 peak = 1103712256
[M::process] read 697148 sequences (100000083 bp) in 2.010 CPU sec, 2.159 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291146, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.62, 123.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 279, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (305.89, 112.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RR
[M::process] read 698300 sequences (100000154 bp) in 1.370 CPU sec, 1.366 real sec...
[M::mem_process_seqs] Processed 698288 reads in 134.792 CPU sec, 4.293 real sec
[M::mem_process_seqs] Processed 697644 reads in 85.311 CPU sec, 2.701 real sec
sorting block 64, #reads = 1090155 mem = 536881731 peak = 1103712256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290456, 8, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (168, 442, 960)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2544)
[M::mem_pestat] mean and std.dev: (512.00, 340.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3336)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679)
[M::mem_pestat] mean and std.dev: (255.83, 124.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 852)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (355, 623, 2389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6457)
[M::mem_pestat] mean and std.dev: (1301.47, 1330.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8491)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 698272 sequences (100000272 bp) in 2.355 CPU sec, 2.487 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290774, 4, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 133, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (158.50, 93.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1223)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.97, 120.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697148 reads in 134.043 CPU sec, 4.259 real sec
[M::process] read 698242 sequences (100000210 bp) in 1.390 CPU sec, 1.396 real sec...
[M::mem_process_seqs] Processed 698300 reads in 85.754 CPU sec, 2.719 real sec
sorting block 65, #reads = 1089601 mem = 536880961 peak = 1103712256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290612, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.99, 121.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701796 sequences (100000069 bp) in 2.033 CPU sec, 2.152 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290728, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.55, 121.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702700 sequences (100000200 bp) in 1.370 CPU sec, 1.371 real sec...
[M::mem_process_seqs] Processed 698272 reads in 137.395 CPU sec, 4.373 real sec
sorting block 66, #reads = 1090970 mem = 536880081 peak = 1103712256
[M::mem_process_seqs] Processed 698242 reads in 88.611 CPU sec, 2.805 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291791, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.27, 116.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 67, #reads = 1089308 mem = 536881500 peak = 1103712256
[M::process] read 701394 sequences (100000222 bp) in 2.314 CPU sec, 2.431 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292726, 12, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (242.32, 117.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (290, 668, 3130)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8810)
[M::mem_pestat] mean and std.dev: (1222.67, 1191.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11650)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 701300 sequences (100000270 bp) in 1.360 CPU sec, 1.362 real sec...
[M::mem_process_seqs] Processed 701796 reads in 143.006 CPU sec, 4.546 real sec
[M::mem_process_seqs] Processed 702700 reads in 86.017 CPU sec, 2.727 real sec
sorting block 68, #reads = 1086689 mem = 536877825 peak = 1103712256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292367, 5, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (244.64, 118.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 469, 770)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1814)
[M::mem_pestat] mean and std.dev: (525.75, 381.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2336)
[M::mem_pestat] skip orientation RR
[M::process] read 698130 sequences (100000264 bp) in 2.031 CPU sec, 2.146 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291824, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (246.75, 120.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701394 reads in 130.586 CPU sec, 4.171 real sec
[M::process] read 701324 sequences (100000121 bp) in 1.523 CPU sec, 1.556 real sec...
[M::mem_process_seqs] Processed 701300 reads in 83.432 CPU sec, 2.631 real sec
sorting block 69, #reads = 1094328 mem = 536881643 peak = 1103712256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291027, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 230, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684)
[M::mem_pestat] mean and std.dev: (255.08, 123.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701660 sequences (100000086 bp) in 2.308 CPU sec, 2.450 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 292353, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.39, 115.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698130 reads in 131.219 CPU sec, 4.182 real sec
[M::process] read 700898 sequences (100000005 bp) in 1.501 CPU sec, 1.524 real sec...
sorting block 70, #reads = 1093621 mem = 536879164 peak = 1103712256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291876, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.59, 115.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700774 sequences (100000105 bp) in 1.438 CPU sec, 1.439 real sec...
[M::mem_process_seqs] Processed 701660 reads in 78.376 CPU sec, 2.662 real sec
[M::mem_process_seqs] Processed 701324 reads in 182.960 CPU sec, 5.977 real sec
sorting block 71, #reads = 1092887 mem = 536878302 peak = 1103712256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291971, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (244.70, 117.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698692 sequences (100000183 bp) in 2.200 CPU sec, 2.352 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 292267, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.27, 118.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700898 reads in 143.523 CPU sec, 4.568 real sec
[M::mem_process_seqs] Processed 700774 reads in 83.862 CPU sec, 2.653 real sec
[M::process] read 700220 sequences (100000153 bp) in 1.643 CPU sec, 1.705 real sec...
sorting block 72, #reads = 1093808 mem = 536878926 peak = 1103712256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291339, 10, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (253.36, 122.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (15, 535, 783)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2319)
[M::mem_pestat] mean and std.dev: (412.10, 396.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3087)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 699720 sequences (100000117 bp) in 2.273 CPU sec, 2.426 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291595, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (254, 318, 1270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3302)
[M::mem_pestat] mean and std.dev: (677.82, 617.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4318)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.85, 118.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 292, 937)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2361)
[M::mem_pestat] mean and std.dev: (329.38, 237.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3073)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698692 reads in 131.042 CPU sec, 4.170 real sec
[M::mem_process_seqs] Processed 700220 reads in 83.551 CPU sec, 2.638 real sec
[M::process] read 699190 sequences (100000025 bp) in 1.492 CPU sec, 1.522 real sec...
sorting block 73, #reads = 1093599 mem = 536879059 peak = 1103712256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291488, 8, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 202, 1772)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4984)
[M::mem_pestat] mean and std.dev: (759.91, 900.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6590)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.34, 119.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 74, #reads = 1093855 mem = 536879344 peak = 1103712256
[M::process] read 699030 sequences (100000054 bp) in 1.988 CPU sec, 2.096 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291815, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.91, 122.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699720 reads in 132.443 CPU sec, 4.231 real sec
[M::process] read 699152 sequences (100000231 bp) in 1.507 CPU sec, 1.527 real sec...
[M::mem_process_seqs] Processed 699190 reads in 84.739 CPU sec, 2.670 real sec
sorting block 75, #reads = 1094165 mem = 536880350 peak = 1103712256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291214, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.76, 120.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698914 sequences (100000016 bp) in 2.273 CPU sec, 2.423 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291217, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.06, 120.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699030 reads in 135.479 CPU sec, 4.310 real sec
[M::process] read 699312 sequences (100000232 bp) in 1.380 CPU sec, 1.383 real sec...
sorting block 76, #reads = 1092501 mem = 536878272 peak = 1103712256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291206, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.79, 120.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 274, 770)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1980)
[M::mem_pestat] mean and std.dev: (370.67, 249.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2585)
[M::mem_pestat] skip orientation RR
[M::process] read 703544 sequences (100000200 bp) in 1.681 CPU sec, 1.688 real sec...
[M::mem_process_seqs] Processed 698914 reads in 79.460 CPU sec, 2.696 real sec
[M::mem_process_seqs] Processed 699152 reads in 190.457 CPU sec, 6.223 real sec
sorting block 77, #reads = 1089804 mem = 536878665 peak = 1103712256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291310, 8, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (245.40, 117.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 804)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703954 sequences (100000283 bp) in 1.969 CPU sec, 2.060 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292153, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (239.58, 116.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704560 sequences (100000243 bp) in 1.400 CPU sec, 1.399 real sec...
[M::mem_process_seqs] Processed 699312 reads in 161.779 CPU sec, 5.140 real sec
[M::mem_process_seqs] Processed 703544 reads in 99.665 CPU sec, 3.162 real sec
sorting block 78, #reads = 1088623 mem = 536882119 peak = 1103712256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292936, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (241.09, 117.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701760 sequences (100000147 bp) in 2.317 CPU sec, 2.451 real sec...
[M::process] read 703632 sequences (100000115 bp) in 1.410 CPU sec, 1.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293913, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 212, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (237.75, 114.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 786)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703954 reads in 149.318 CPU sec, 4.744 real sec
[M::mem_process_seqs] Processed 704560 reads in 93.635 CPU sec, 2.977 real sec
sorting block 79, #reads = 1084138 mem = 536883201 peak = 1103712256
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292268, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 220, 950)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2482)
[M::mem_pestat] mean and std.dev: (470.85, 436.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3248)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (246.85, 120.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 704410 sequences (100000281 bp) in 2.097 CPU sec, 2.240 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293200, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (240.57, 116.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703098 sequences (100000169 bp) in 1.400 CPU sec, 1.401 real sec...
[M::mem_process_seqs] Processed 701760 reads in 142.742 CPU sec, 4.547 real sec
sorting block 80, #reads = 1089681 mem = 536884204 peak = 1103712256
[M::mem_process_seqs] Processed 703632 reads in 89.585 CPU sec, 2.840 real sec
sorting block 81, #reads = 1090021 mem = 536880250 peak = 1135067136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 293318, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 250, 651)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1503)
[M::mem_pestat] mean and std.dev: (396.08, 339.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1929)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (236.82, 113.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 782)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701420 sequences (100000065 bp) in 2.292 CPU sec, 2.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292803, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (238.82, 113.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699370 sequences (100000142 bp) in 1.370 CPU sec, 1.368 real sec...
[M::mem_process_seqs] Processed 704410 reads in 141.322 CPU sec, 4.492 real sec
[M::mem_process_seqs] Processed 703098 reads in 89.734 CPU sec, 2.848 real sec
sorting block 82, #reads = 1092363 mem = 536880336 peak = 1135067136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291825, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.20, 116.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701396 sequences (100000012 bp) in 2.002 CPU sec, 2.098 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291541, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 244, 278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 416)
[M::mem_pestat] mean and std.dev: (243.92, 36.25)
[M::mem_pestat] low and high boundaries for proper pairs: (2, 485)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (249.89, 120.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700068 sequences (100000241 bp) in 1.380 CPU sec, 1.374 real sec...
[M::mem_process_seqs] Processed 701420 reads in 137.529 CPU sec, 4.386 real sec
[M::mem_process_seqs] Processed 699370 reads in 87.251 CPU sec, 2.765 real sec
sorting block 83, #reads = 1090592 mem = 536879262 peak = 1135067136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292122, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (117, 260, 260)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546)
[M::mem_pestat] mean and std.dev: (209.30, 78.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 689)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (244.13, 117.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 699930 sequences (100000249 bp) in 2.185 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 291849, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (246.96, 118.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699410 sequences (100000221 bp) in 1.350 CPU sec, 1.351 real sec...
[M::mem_process_seqs] Processed 701396 reads in 136.433 CPU sec, 4.341 real sec
sorting block 84, #reads = 1090147 mem = 536885738 peak = 1135067136
[M::mem_process_seqs] Processed 700068 reads in 86.446 CPU sec, 2.740 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291643, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (179, 379, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (268.22, 112.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.19, 117.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 85, #reads = 1091868 mem = 536882959 peak = 1135067136
[M::process] read 698936 sequences (100000076 bp) in 2.094 CPU sec, 2.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291417, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.45, 119.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699930 reads in 134.305 CPU sec, 4.287 real sec
[M::process] read 698786 sequences (100000255 bp) in 1.465 CPU sec, 1.479 real sec...
[M::mem_process_seqs] Processed 699410 reads in 86.074 CPU sec, 2.720 real sec
sorting block 86, #reads = 1092681 mem = 536879340 peak = 1135067136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291653, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.74, 119.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 247, 480)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992)
[M::mem_pestat] mean and std.dev: (257.70, 77.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RR
[M::process] read 698196 sequences (100000158 bp) in 2.039 CPU sec, 2.170 real sec...
[M::process] read 256430 sequences (36613076 bp) in 0.700 CPU sec, 0.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291057, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.86, 119.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698936 reads in 133.615 CPU sec, 4.281 real sec
[M::mem_process_seqs] Processed 698786 reads in 85.273 CPU sec, 2.715 real sec
sorting block 87, #reads = 1092689 mem = 536877256 peak = 1135067136
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290876, 7, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (173, 474, 725)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1829)
[M::mem_pestat] mean and std.dev: (420.40, 268.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2381)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.40, 120.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 107130, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (243.71, 116.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698196 reads in 95.163 CPU sec, 3.107 real sec
[M::mem_process_seqs] Processed 256430 reads in 39.199 CPU sec, 1.274 real sec
sorting block 88, #reads = 1091743 mem = 536878263 peak = 1135947776
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 97374742
overall: 70055100416 mem 11284.027 user 327.125 sys 861.294 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L008\tPU:L008\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0001.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 861.329 sec; CPU: 11611.154 sec
sorting block 89, #reads = 649823 mem = 319951565 peak = 1135947776
uniq header count 1
merging 90 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5228802535
output reads: 97869503
bam_mem_sort: 90 calls 34.321 user 0.173 sys 34.722 real
bam_write: 90 calls 55.080 user 1.236 sys 56.710 real
execute: 1 calls 17.332 user 0.938 sys 888.929 real
merge_files: 1 calls 17.311 user 0.911 sys 26.755 real
parse_chunk: 1434 calls 45.475 user 13.402 sys 60.053 real
read_chunk: 3872 calls 36.999 user 33.187 sys 334.025 real
sort_block: 1 calls 28.956 user 32.645 sys 322.196 real
write_chunk: 2436 calls 16.946 user 0.726 sys 25.927 real
overall: 3072303104 mem 1046.747 user 57.362 sys 888.949 real
ls: cannot access '*.cram': No such file or directory