File Info

Filename
.command.err
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/fe/12349fb7c7fbeddbad1817b443c2ff/.command.err
Size
204.3 KB
Task
DAQ:SENTIEON_BWAMEM (659_cfy-T1-TDNA-1_A23MHWWLT4_1;L006;0001)
Attempt

Content

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This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_cfy-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2825.355 CPU sec, 116.962 real sec
[M::process] read 691282 sequences (100000294 bp) in 1.130 CPU sec, 1.141 real sec...
[M::process] read 688624 sequences (100000131 bp) in 1.657 CPU sec, 1.688 real sec...
[M::process] read 687966 sequences (100000221 bp) in 1.723 CPU sec, 1.746 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288636, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 253, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (277.13, 133.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 922)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687592 sequences (100000144 bp) in 1.633 CPU sec, 1.656 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286917, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 268, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (290.01, 137.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287349, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 265, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (287.98, 137.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 691282 reads in 300.820 CPU sec, 9.664 real sec
[M::mem_process_seqs] Processed 687966 reads in 197.080 CPU sec, 6.400 real sec
[M::mem_process_seqs] Processed 688624 reads in 213.705 CPU sec, 6.943 real sec
sorting block 0, #reads = 1089568 mem = 536878932 peak = 582320128
[M::process] read 687462 sequences (100000208 bp) in 2.406 CPU sec, 2.567 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286586, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (292.33, 139.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687138 sequences (100000068 bp) in 1.988 CPU sec, 2.003 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287073, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (293.46, 139.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 980)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686678 sequences (100000196 bp) in 1.690 CPU sec, 1.698 real sec...
[M::mem_process_seqs] Processed 687592 reads in 185.560 CPU sec, 5.872 real sec
sorting block 1, #reads = 1088041 mem = 536878205 peak = 874872832
[M::mem_process_seqs] Processed 687462 reads in 115.416 CPU sec, 3.633 real sec
sorting block 2, #reads = 1088313 mem = 536879311 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286868, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 686294 sequences (100000211 bp) in 2.356 CPU sec, 2.434 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (185, 273, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (294.38, 139.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685992 sequences (100000073 bp) in 1.884 CPU sec, 1.903 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286418, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 275, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (296.03, 140.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687138 reads in 180.090 CPU sec, 5.713 real sec
[M::mem_process_seqs] Processed 686678 reads in 113.153 CPU sec, 3.598 real sec
sorting block 3, #reads = 1087993 mem = 536882649 peak = 874872832
[M::process] read 685804 sequences (100000156 bp) in 2.210 CPU sec, 2.302 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286779, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 278, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (299.07, 141.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685478 sequences (100000179 bp) in 1.751 CPU sec, 1.755 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286220, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (300.35, 142.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286108, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (300.71, 142.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686294 reads in 253.659 CPU sec, 8.111 real sec
[M::mem_process_seqs] Processed 685992 reads in 187.627 CPU sec, 6.008 real sec
sorting block 4, #reads = 1087921 mem = 536880281 peak = 874872832
[M::mem_process_seqs] Processed 685804 reads in 204.422 CPU sec, 6.574 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286792, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (298.96, 141.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 5, #reads = 1087350 mem = 536879976 peak = 874872832
[M::process] read 685258 sequences (100000019 bp) in 2.737 CPU sec, 2.931 real sec...
[M::mem_process_seqs] Processed 685478 reads in 98.486 CPU sec, 3.233 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286027, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 685474 sequences (100000130 bp) in 2.763 CPU sec, 2.969 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (303.08, 143.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684770 sequences (100000260 bp) in 1.946 CPU sec, 2.057 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286129, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (303.00, 143.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685258 reads in 177.533 CPU sec, 5.663 real sec
sorting block 6, #reads = 1088601 mem = 536877052 peak = 874872832
[M::mem_process_seqs] Processed 685474 reads in 113.296 CPU sec, 3.572 real sec
[M::process] read 684954 sequences (100000158 bp) in 2.423 CPU sec, 2.525 real sec...
sorting block 7, #reads = 1087051 mem = 536877930 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286502, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 283, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (304.81, 143.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684902 sequences (100000196 bp) in 2.373 CPU sec, 2.494 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286176, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (304.79, 144.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685150 sequences (100000219 bp) in 1.819 CPU sec, 1.820 real sec...
[M::mem_process_seqs] Processed 684770 reads in 180.286 CPU sec, 5.728 real sec
[M::mem_process_seqs] Processed 684954 reads in 114.938 CPU sec, 3.629 real sec
sorting block 8, #reads = 1086963 mem = 536878775 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285990, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 283, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (303.79, 143.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684872 sequences (100000005 bp) in 2.694 CPU sec, 2.806 real sec...
[M::process] read 684430 sequences (100000208 bp) in 1.839 CPU sec, 1.841 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285684, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (303.92, 144.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684902 reads in 180.463 CPU sec, 5.731 real sec
[M::mem_process_seqs] Processed 685150 reads in 113.823 CPU sec, 3.607 real sec
sorting block 9, #reads = 1087381 mem = 536881450 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286687, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (303.48, 143.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684400 sequences (100000281 bp) in 2.501 CPU sec, 2.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285695, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 283, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (303.37, 142.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684634 sequences (100000094 bp) in 2.048 CPU sec, 2.055 real sec...
[M::mem_process_seqs] Processed 684872 reads in 182.072 CPU sec, 5.779 real sec
sorting block 10, #reads = 1086612 mem = 536877470 peak = 874872832
[M::mem_process_seqs] Processed 684430 reads in 115.717 CPU sec, 3.663 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286441, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (302.14, 140.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 11, #reads = 1085320 mem = 536879402 peak = 874872832
[M::process] read 684492 sequences (100000014 bp) in 2.924 CPU sec, 3.049 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286026, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 281, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (301.07, 140.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684464 sequences (100000215 bp) in 2.014 CPU sec, 2.021 real sec...
[M::mem_process_seqs] Processed 684400 reads in 181.991 CPU sec, 5.769 real sec
[M::mem_process_seqs] Processed 684634 reads in 114.252 CPU sec, 3.607 real sec
sorting block 12, #reads = 1085862 mem = 536881515 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285457, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 280, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (299.74, 139.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684858 sequences (100000000 bp) in 2.811 CPU sec, 2.969 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285783, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 278, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (298.24, 137.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684678 sequences (100000207 bp) in 2.050 CPU sec, 2.048 real sec...
[M::mem_process_seqs] Processed 684492 reads in 181.159 CPU sec, 5.747 real sec
[M::mem_process_seqs] Processed 684464 reads in 113.825 CPU sec, 3.596 real sec
sorting block 13, #reads = 1086043 mem = 536876302 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286152, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 277, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (297.56, 138.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684762 sequences (100000101 bp) in 2.712 CPU sec, 2.842 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286154, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 278, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (298.23, 138.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684330 sequences (100000104 bp) in 2.081 CPU sec, 2.089 real sec...
[M::mem_process_seqs] Processed 684858 reads in 182.238 CPU sec, 5.784 real sec
[M::mem_process_seqs] Processed 684678 reads in 114.474 CPU sec, 3.620 real sec
sorting block 14, #reads = 1086187 mem = 536878092 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285762, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 278, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (297.56, 137.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684872 sequences (100000126 bp) in 2.484 CPU sec, 2.618 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285875, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 279, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (298.26, 137.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684728 sequences (100000123 bp) in 2.054 CPU sec, 2.058 real sec...
[M::mem_process_seqs] Processed 684762 reads in 180.750 CPU sec, 5.742 real sec
sorting block 15, #reads = 1085162 mem = 536879724 peak = 874872832
[M::mem_process_seqs] Processed 684330 reads in 113.852 CPU sec, 3.604 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286404, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 278, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (297.83, 137.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 16, #reads = 1085576 mem = 536879230 peak = 874872832
[M::process] read 684204 sequences (100000145 bp) in 2.905 CPU sec, 3.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285021, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 279, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (298.65, 138.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684162 sequences (100000116 bp) in 2.013 CPU sec, 2.023 real sec...
[M::mem_process_seqs] Processed 684872 reads in 181.300 CPU sec, 5.755 real sec
[M::mem_process_seqs] Processed 684728 reads in 114.772 CPU sec, 3.627 real sec
sorting block 17, #reads = 1085729 mem = 536879942 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285642, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (301.62, 139.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683796 sequences (100000176 bp) in 2.751 CPU sec, 2.872 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285810, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 285, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (303.45, 140.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685088 sequences (100000117 bp) in 2.104 CPU sec, 2.109 real sec...
[M::mem_process_seqs] Processed 684204 reads in 184.748 CPU sec, 5.871 real sec
[M::mem_process_seqs] Processed 684162 reads in 116.597 CPU sec, 3.678 real sec
sorting block 18, #reads = 1083791 mem = 536879937 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285614, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 286, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (304.99, 141.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688802 sequences (100000289 bp) in 3.280 CPU sec, 3.407 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285558, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 280, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (300.00, 139.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688534 sequences (100000027 bp) in 1.915 CPU sec, 1.922 real sec...
[M::mem_process_seqs] Processed 683796 reads in 186.846 CPU sec, 5.934 real sec
sorting block 19, #reads = 1084015 mem = 536880013 peak = 874872832
[M::mem_process_seqs] Processed 685088 reads in 120.285 CPU sec, 3.804 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287200, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 265, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (288.44, 138.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687736 sequences (100000124 bp) in 2.742 CPU sec, 2.873 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286789, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 266, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (289.54, 139.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687402 sequences (100000082 bp) in 2.034 CPU sec, 2.042 real sec...
[M::mem_process_seqs] Processed 688802 reads in 180.810 CPU sec, 5.747 real sec
sorting block 20, #reads = 1084708 mem = 536880777 peak = 874872832
[M::mem_process_seqs] Processed 688534 reads in 113.098 CPU sec, 3.572 real sec
sorting block 21, #reads = 1089935 mem = 536877886 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286383, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (292.23, 139.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687116 sequences (100000090 bp) in 3.068 CPU sec, 3.171 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286242, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 271, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (293.57, 139.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 984)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687736 reads in 180.094 CPU sec, 5.722 real sec
[M::process] read 686878 sequences (100000051 bp) in 2.010 CPU sec, 2.011 real sec...
[M::mem_process_seqs] Processed 687402 reads in 114.159 CPU sec, 3.601 real sec
sorting block 22, #reads = 1089410 mem = 536876599 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286715, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (294.37, 139.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686746 sequences (100000082 bp) in 2.490 CPU sec, 2.627 real sec...
[M::process] read 686336 sequences (100000181 bp) in 1.640 CPU sec, 1.638 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286499, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (296.63, 140.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 994)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687116 reads in 174.039 CPU sec, 5.545 real sec
[M::mem_process_seqs] Processed 686878 reads in 108.348 CPU sec, 3.438 real sec
sorting block 23, #reads = 1088829 mem = 536880100 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286622, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (297.48, 141.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686140 sequences (100000300 bp) in 2.758 CPU sec, 2.887 real sec...
[M::process] read 685998 sequences (100000202 bp) in 1.800 CPU sec, 1.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286323, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 277, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (298.74, 141.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686746 reads in 178.842 CPU sec, 5.687 real sec
sorting block 24, #reads = 1088357 mem = 536880827 peak = 874872832
[M::mem_process_seqs] Processed 686336 reads in 112.686 CPU sec, 3.579 real sec
[M::process] read 685784 sequences (100000088 bp) in 2.602 CPU sec, 2.737 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286475, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 280, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (301.37, 143.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685542 sequences (100000176 bp) in 1.830 CPU sec, 1.836 real sec...
[M::mem_process_seqs] Processed 685998 reads in 102.244 CPU sec, 3.242 real sec
sorting block 25, #reads = 1088173 mem = 536878455 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286139, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (300.67, 142.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285712, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 280, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (301.28, 142.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686140 reads in 274.571 CPU sec, 8.741 real sec
[M::process] read 685456 sequences (100000124 bp) in 2.086 CPU sec, 2.093 real sec...
[M::mem_process_seqs] Processed 685784 reads in 128.131 CPU sec, 4.087 real sec
sorting block 26, #reads = 1088904 mem = 536878548 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286243, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (301.76, 141.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685268 sequences (100000071 bp) in 3.004 CPU sec, 3.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286035, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 283, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (303.84, 143.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685542 reads in 177.077 CPU sec, 5.646 real sec
sorting block 27, #reads = 1087568 mem = 536875830 peak = 874872832
[M::process] read 685142 sequences (100000216 bp) in 2.045 CPU sec, 2.079 real sec...
[M::mem_process_seqs] Processed 685456 reads in 110.785 CPU sec, 3.492 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286143, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 283, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (304.87, 144.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1030)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 28, #reads = 1087546 mem = 536878351 peak = 874872832
[M::process] read 684744 sequences (100000271 bp) in 3.001 CPU sec, 3.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286162, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (305.58, 144.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685268 reads in 176.807 CPU sec, 5.618 real sec
[M::process] read 684410 sequences (100000127 bp) in 2.007 CPU sec, 2.005 real sec...
[M::mem_process_seqs] Processed 685142 reads in 112.245 CPU sec, 3.543 real sec
sorting block 29, #reads = 1087642 mem = 536880701 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286004, 10, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 284, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (305.05, 144.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (711, 711, 711)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (711, 711)
[M::mem_pestat] mean and std.dev: (711.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (711, 711)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 684388 sequences (100000031 bp) in 2.942 CPU sec, 3.117 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285746, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (306.10, 143.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684744 reads in 176.572 CPU sec, 5.612 real sec
[M::process] read 684502 sequences (100000217 bp) in 1.971 CPU sec, 1.977 real sec...
[M::mem_process_seqs] Processed 684410 reads in 111.100 CPU sec, 3.505 real sec
sorting block 30, #reads = 1087087 mem = 536880104 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285586, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (306.96, 144.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1025)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684480 sequences (100000076 bp) in 2.810 CPU sec, 2.939 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286293, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (304.53, 142.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684392 sequences (100000060 bp) in 1.934 CPU sec, 1.942 real sec...
[M::mem_process_seqs] Processed 684388 reads in 179.893 CPU sec, 5.715 real sec
[M::mem_process_seqs] Processed 684502 reads in 114.615 CPU sec, 3.629 real sec
sorting block 31, #reads = 1087287 mem = 536876552 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286060, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 280, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (298.66, 137.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 975)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684476 sequences (100000070 bp) in 3.045 CPU sec, 3.179 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285460, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (301.31, 140.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 992)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684290 sequences (100000212 bp) in 2.080 CPU sec, 2.087 real sec...
[M::mem_process_seqs] Processed 684480 reads in 185.364 CPU sec, 5.898 real sec
sorting block 32, #reads = 1085997 mem = 536879270 peak = 874872832
[M::mem_process_seqs] Processed 684392 reads in 117.272 CPU sec, 3.702 real sec
sorting block 33, #reads = 1085203 mem = 536881670 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285624, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (300.91, 139.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684800 sequences (100000104 bp) in 2.875 CPU sec, 3.014 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285618, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 280, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (299.98, 139.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684618 sequences (100000034 bp) in 2.049 CPU sec, 2.051 real sec...
[M::mem_process_seqs] Processed 684476 reads in 181.668 CPU sec, 5.769 real sec
[M::mem_process_seqs] Processed 684290 reads in 114.413 CPU sec, 3.619 real sec
sorting block 34, #reads = 1086198 mem = 536879985 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285998, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 279, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (298.44, 138.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684702 sequences (100000226 bp) in 2.794 CPU sec, 2.909 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285679, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (299.81, 139.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684898 sequences (100000130 bp) in 2.060 CPU sec, 2.050 real sec...
[M::mem_process_seqs] Processed 684800 reads in 173.930 CPU sec, 5.513 real sec
[M::mem_process_seqs] Processed 684618 reads in 110.260 CPU sec, 3.478 real sec
sorting block 35, #reads = 1086203 mem = 536880196 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285725, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (300.77, 139.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684550 sequences (100000123 bp) in 2.870 CPU sec, 2.989 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286310, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 280, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (299.45, 139.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684902 sequences (100000171 bp) in 1.850 CPU sec, 1.842 real sec...
[M::mem_process_seqs] Processed 684702 reads in 174.136 CPU sec, 5.526 real sec
[M::mem_process_seqs] Processed 684898 reads in 108.854 CPU sec, 3.445 real sec
sorting block 36, #reads = 1085397 mem = 536880099 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286024, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 280, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (299.61, 138.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 37, #reads = 1086103 mem = 536879337 peak = 874872832
[M::process] read 684746 sequences (100000020 bp) in 2.824 CPU sec, 2.933 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285949, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 280, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (299.47, 139.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684810 sequences (100000169 bp) in 2.030 CPU sec, 2.032 real sec...
[M::mem_process_seqs] Processed 684550 reads in 176.488 CPU sec, 5.596 real sec
[M::mem_process_seqs] Processed 684902 reads in 111.760 CPU sec, 3.522 real sec
sorting block 38, #reads = 1085397 mem = 536879157 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285926, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791)
[M::mem_pestat] mean and std.dev: (300.49, 139.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684420 sequences (100000159 bp) in 2.797 CPU sec, 2.900 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286322, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (302.49, 141.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685282 sequences (100000296 bp) in 2.040 CPU sec, 2.033 real sec...
[M::mem_process_seqs] Processed 684746 reads in 182.056 CPU sec, 5.774 real sec
[M::mem_process_seqs] Processed 684810 reads in 115.588 CPU sec, 3.649 real sec
sorting block 39, #reads = 1085037 mem = 536879139 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285863, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (303.89, 142.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689348 sequences (100000053 bp) in 2.871 CPU sec, 2.972 real sec...
[M::process] read 689264 sequences (100000124 bp) in 1.958 CPU sec, 1.952 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286419, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (301.18, 141.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (467, 467, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (467, 467)
[M::mem_pestat] mean and std.dev: (467.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (467, 467)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 684420 reads in 184.096 CPU sec, 5.859 real sec
[M::mem_process_seqs] Processed 685282 reads in 117.357 CPU sec, 3.722 real sec
sorting block 40, #reads = 1084229 mem = 536880164 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 287434, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 263, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (286.38, 137.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688928 sequences (100000185 bp) in 2.908 CPU sec, 3.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286891, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 264, 375)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (286.53, 137.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688748 sequences (100000244 bp) in 1.989 CPU sec, 1.999 real sec...
[M::mem_process_seqs] Processed 689348 reads in 178.441 CPU sec, 5.659 real sec
sorting block 41, #reads = 1084433 mem = 536877635 peak = 874872832
[M::mem_process_seqs] Processed 689264 reads in 113.820 CPU sec, 3.601 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286960, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 264, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768)
[M::mem_pestat] mean and std.dev: (287.49, 137.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 42, #reads = 1089206 mem = 536883643 peak = 874872832
[M::process] read 688562 sequences (100000187 bp) in 2.903 CPU sec, 3.031 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287145, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 265, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (288.16, 137.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688260 sequences (100000133 bp) in 1.990 CPU sec, 1.990 real sec...
[M::mem_process_seqs] Processed 688928 reads in 177.754 CPU sec, 5.638 real sec
[M::mem_process_seqs] Processed 688748 reads in 112.478 CPU sec, 3.552 real sec
sorting block 43, #reads = 1089433 mem = 536878983 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287357, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (289.46, 138.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687950 sequences (100000151 bp) in 2.809 CPU sec, 2.934 real sec...
[M::process] read 687654 sequences (100000037 bp) in 1.671 CPU sec, 1.678 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286959, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 268, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (291.59, 140.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688562 reads in 174.462 CPU sec, 5.548 real sec
[M::mem_process_seqs] Processed 688260 reads in 110.351 CPU sec, 3.501 real sec
sorting block 44, #reads = 1089954 mem = 536877769 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286872, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 269, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (292.34, 140.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686698 sequences (100000019 bp) in 2.979 CPU sec, 3.090 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286838, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 272, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (295.14, 141.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687270 sequences (100000166 bp) in 2.059 CPU sec, 2.058 real sec...
[M::mem_process_seqs] Processed 687950 reads in 179.183 CPU sec, 5.687 real sec
[M::mem_process_seqs] Processed 687654 reads in 113.571 CPU sec, 3.585 real sec
sorting block 45, #reads = 1088700 mem = 536878645 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286207, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 275, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795)
[M::mem_pestat] mean and std.dev: (296.84, 141.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686088 sequences (100000158 bp) in 3.016 CPU sec, 3.134 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287146, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (295.18, 141.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686374 sequences (100000011 bp) in 2.004 CPU sec, 2.007 real sec...
[M::mem_process_seqs] Processed 686698 reads in 176.554 CPU sec, 5.594 real sec
sorting block 46, #reads = 1088795 mem = 536882550 peak = 874872832
[M::mem_process_seqs] Processed 687270 reads in 112.183 CPU sec, 3.543 real sec
sorting block 47, #reads = 1088394 mem = 536877162 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286689, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 278, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (300.11, 142.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685792 sequences (100000171 bp) in 2.856 CPU sec, 2.959 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286744, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 276, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (298.88, 142.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686088 reads in 172.964 CPU sec, 5.487 real sec
[M::process] read 685850 sequences (100000249 bp) in 1.984 CPU sec, 1.990 real sec...
[M::mem_process_seqs] Processed 686374 reads in 110.434 CPU sec, 3.484 real sec
sorting block 48, #reads = 1088405 mem = 536878536 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286263, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 281, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (302.19, 143.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685842 sequences (100000276 bp) in 2.901 CPU sec, 3.003 real sec...
[M::process] read 685822 sequences (100000290 bp) in 1.490 CPU sec, 1.492 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286473, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 280, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (302.74, 145.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685792 reads in 173.898 CPU sec, 5.530 real sec
[M::mem_process_seqs] Processed 685850 reads in 111.008 CPU sec, 3.525 real sec
sorting block 49, #reads = 1088401 mem = 536877056 peak = 874872832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286025, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (302.33, 145.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685578 sequences (100000087 bp) in 3.134 CPU sec, 3.251 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285920, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 280, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (302.62, 144.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685202 sequences (100000148 bp) in 1.990 CPU sec, 1.998 real sec...
[M::mem_process_seqs] Processed 685842 reads in 175.313 CPU sec, 5.558 real sec
sorting block 50, #reads = 1087945 mem = 536877879 peak = 874872832
[M::mem_process_seqs] Processed 685822 reads in 109.607 CPU sec, 3.466 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286076, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 281, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813)
[M::mem_pestat] mean and std.dev: (302.74, 143.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1021)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685998 sequences (100000102 bp) in 2.916 CPU sec, 3.022 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285964, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 282, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (303.21, 143.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685954 sequences (100000202 bp) in 1.896 CPU sec, 1.903 real sec...
[M::mem_process_seqs] Processed 685578 reads in 176.329 CPU sec, 5.592 real sec
sorting block 51, #reads = 1087951 mem = 536887515 peak = 874872832
[M::mem_process_seqs] Processed 685202 reads in 112.289 CPU sec, 3.549 real sec
sorting block 52, #reads = 1086566 mem = 536879063 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286332, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 273, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (294.33, 138.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685994 sequences (100000180 bp) in 2.689 CPU sec, 2.803 real sec...
[M::process] read 685190 sequences (100000028 bp) in 1.700 CPU sec, 1.696 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286359, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 274, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (296.28, 139.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685998 reads in 177.588 CPU sec, 5.649 real sec
[M::mem_process_seqs] Processed 685954 reads in 112.074 CPU sec, 3.553 real sec
sorting block 53, #reads = 1086936 mem = 536877391 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286489, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 274, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (295.98, 139.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684420 sequences (100000222 bp) in 3.064 CPU sec, 3.175 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286197, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (302.40, 143.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684414 sequences (100000238 bp) in 1.715 CPU sec, 1.720 real sec...
[M::mem_process_seqs] Processed 685994 reads in 176.506 CPU sec, 5.603 real sec
[M::mem_process_seqs] Processed 685190 reads in 113.133 CPU sec, 3.581 real sec
sorting block 54, #reads = 1086044 mem = 536878489 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285151, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (304.30, 142.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684602 sequences (100000143 bp) in 2.748 CPU sec, 2.865 real sec...
[M::process] read 684486 sequences (100000163 bp) in 1.540 CPU sec, 1.532 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285549, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 282, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (302.85, 141.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1000)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684420 reads in 175.593 CPU sec, 5.587 real sec
sorting block 55, #reads = 1086992 mem = 536881591 peak = 908648448
[M::mem_process_seqs] Processed 684414 reads in 110.808 CPU sec, 3.524 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286129, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 283, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (303.59, 143.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1084824 mem = 536881254 peak = 908648448
[M::process] read 684746 sequences (100000226 bp) in 2.900 CPU sec, 2.981 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286175, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 283, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (303.94, 143.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684678 sequences (100000188 bp) in 1.850 CPU sec, 1.854 real sec...
[M::mem_process_seqs] Processed 684602 reads in 172.037 CPU sec, 5.455 real sec
[M::mem_process_seqs] Processed 684486 reads in 109.846 CPU sec, 3.469 real sec
sorting block 57, #reads = 1086140 mem = 536877641 peak = 908648448
[M::process] read 684730 sequences (100000202 bp) in 3.046 CPU sec, 3.114 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285505, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 281, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (301.99, 141.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685130 sequences (100000227 bp) in 2.050 CPU sec, 2.049 real sec...
[M::mem_process_seqs] Processed 684678 reads in 101.878 CPU sec, 3.215 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285791, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (302.73, 142.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285985, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (302.28, 141.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684282 sequences (100000006 bp) in 2.018 CPU sec, 2.022 real sec...
[M::mem_process_seqs] Processed 684746 reads in 276.563 CPU sec, 8.758 real sec
sorting block 58, #reads = 1085736 mem = 536879605 peak = 908648448
[M::mem_process_seqs] Processed 684730 reads in 129.276 CPU sec, 4.112 real sec
sorting block 59, #reads = 1085112 mem = 536878473 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285624, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (299.70, 141.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683944 sequences (100000212 bp) in 3.067 CPU sec, 3.156 real sec...
[M::process] read 688142 sequences (100000153 bp) in 1.500 CPU sec, 1.501 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285529, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 285, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819)
[M::mem_pestat] mean and std.dev: (306.36, 144.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685130 reads in 177.408 CPU sec, 5.638 real sec
[M::mem_process_seqs] Processed 684282 reads in 110.007 CPU sec, 3.485 real sec
sorting block 60, #reads = 1083920 mem = 536879273 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285154, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 287, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823)
[M::mem_pestat] mean and std.dev: (307.94, 145.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689170 sequences (100000160 bp) in 2.678 CPU sec, 2.836 real sec...
[M::process] read 688866 sequences (100000072 bp) in 1.520 CPU sec, 1.520 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287018, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 268, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (291.55, 139.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 249, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945)
[M::mem_pestat] mean and std.dev: (334.08, 154.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1192)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 683944 reads in 171.912 CPU sec, 5.463 real sec
[M::mem_process_seqs] Processed 688142 reads in 107.481 CPU sec, 3.409 real sec
sorting block 61, #reads = 1083491 mem = 536880256 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286913, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 264, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (287.30, 138.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688352 sequences (100000027 bp) in 2.882 CPU sec, 2.993 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287091, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 265, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (288.16, 138.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689170 reads in 162.518 CPU sec, 5.154 real sec
[M::process] read 688216 sequences (100000294 bp) in 2.031 CPU sec, 2.049 real sec...
[M::mem_process_seqs] Processed 688866 reads in 101.430 CPU sec, 3.186 real sec
sorting block 62, #reads = 1088675 mem = 536877913 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286926, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 266, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769)
[M::mem_pestat] mean and std.dev: (288.59, 137.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 965)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 63, #reads = 1090137 mem = 536877667 peak = 908648448
[M::process] read 688100 sequences (100000020 bp) in 2.329 CPU sec, 2.442 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286859, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (289.85, 138.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688252 sequences (100000159 bp) in 1.950 CPU sec, 1.951 real sec...
[M::mem_process_seqs] Processed 688352 reads in 158.669 CPU sec, 5.033 real sec
[M::mem_process_seqs] Processed 688216 reads in 98.855 CPU sec, 3.116 real sec
sorting block 64, #reads = 1090281 mem = 536876740 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286695, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 268, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (290.67, 138.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 978)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687826 sequences (100000037 bp) in 2.319 CPU sec, 2.430 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286918, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 266, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (290.08, 139.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687684 sequences (100000268 bp) in 1.930 CPU sec, 1.932 real sec...
[M::mem_process_seqs] Processed 688100 reads in 159.554 CPU sec, 5.068 real sec
[M::mem_process_seqs] Processed 688252 reads in 98.862 CPU sec, 3.117 real sec
sorting block 65, #reads = 1088952 mem = 536882411 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287014, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 270, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (292.47, 140.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687288 sequences (100000150 bp) in 3.010 CPU sec, 3.131 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286533, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789)
[M::mem_pestat] mean and std.dev: (293.77, 140.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687826 reads in 162.110 CPU sec, 5.139 real sec
[M::process] read 687322 sequences (100000285 bp) in 1.890 CPU sec, 1.919 real sec...
[M::mem_process_seqs] Processed 687684 reads in 102.213 CPU sec, 3.216 real sec
sorting block 66, #reads = 1089354 mem = 536880756 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286384, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 272, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 792)
[M::mem_pestat] mean and std.dev: (294.43, 141.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 995)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687016 sequences (100000149 bp) in 2.620 CPU sec, 2.732 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286455, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 271, 385)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (293.86, 140.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687288 reads in 163.016 CPU sec, 5.182 real sec
[M::mem_process_seqs] Processed 687322 reads in 103.687 CPU sec, 3.262 real sec
[M::process] read 686710 sequences (100000138 bp) in 2.110 CPU sec, 2.127 real sec...
sorting block 67, #reads = 1088762 mem = 536880256 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286482, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (297.06, 142.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 68, #reads = 1089128 mem = 536878700 peak = 908648448
[M::process] read 686206 sequences (100000155 bp) in 2.251 CPU sec, 2.458 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286241, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (298.35, 143.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686362 sequences (100000176 bp) in 1.820 CPU sec, 1.826 real sec...
[M::mem_process_seqs] Processed 687016 reads in 164.562 CPU sec, 5.236 real sec
[M::mem_process_seqs] Processed 686710 reads in 102.657 CPU sec, 3.240 real sec
sorting block 69, #reads = 1089647 mem = 536879579 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286049, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (299.90, 143.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686154 sequences (100000119 bp) in 2.833 CPU sec, 2.961 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286363, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 279, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (301.71, 145.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686206 reads in 167.202 CPU sec, 5.298 real sec
[M::process] read 685972 sequences (100000238 bp) in 1.989 CPU sec, 1.987 real sec...
[M::mem_process_seqs] Processed 686362 reads in 105.724 CPU sec, 3.332 real sec
sorting block 70, #reads = 1087237 mem = 536879870 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285680, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 278, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (301.46, 144.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686024 sequences (100000299 bp) in 2.797 CPU sec, 2.907 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286392, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (302.29, 144.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686154 reads in 165.357 CPU sec, 5.235 real sec
[M::process] read 685202 sequences (100000045 bp) in 1.970 CPU sec, 1.968 real sec...
[M::mem_process_seqs] Processed 685972 reads in 102.629 CPU sec, 3.237 real sec
sorting block 71, #reads = 1088238 mem = 536876864 peak = 908648448
[M::process] read 685336 sequences (100000223 bp) in 2.787 CPU sec, 2.908 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286156, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 283, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814)
[M::mem_pestat] mean and std.dev: (304.24, 144.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685244 sequences (100000029 bp) in 1.920 CPU sec, 1.923 real sec...
[M::mem_process_seqs] Processed 685202 reads in 96.550 CPU sec, 3.039 real sec
sorting block 72, #reads = 1088269 mem = 536877736 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286367, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 280, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818)
[M::mem_pestat] mean and std.dev: (302.90, 145.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1028)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286445, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (302.83, 143.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686024 reads in 257.136 CPU sec, 8.155 real sec
[M::process] read 684744 sequences (100000044 bp) in 2.051 CPU sec, 2.068 real sec...
[M::mem_process_seqs] Processed 685336 reads in 119.651 CPU sec, 3.794 real sec
sorting block 73, #reads = 1087606 mem = 536878572 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286508, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 281, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (302.39, 142.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684556 sequences (100000227 bp) in 3.147 CPU sec, 3.357 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285870, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 284, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (303.72, 142.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685244 reads in 169.871 CPU sec, 5.389 real sec
sorting block 74, #reads = 1086566 mem = 536878510 peak = 908648448
[M::process] read 684608 sequences (100000122 bp) in 2.123 CPU sec, 2.173 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285930, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 285, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (305.49, 143.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 684358 sequences (100000086 bp) in 2.214 CPU sec, 2.219 real sec...
[M::mem_process_seqs] Processed 684556 reads in 97.187 CPU sec, 3.071 real sec
sorting block 75, #reads = 1087293 mem = 536877215 peak = 908648448
[M::mem_process_seqs] Processed 684744 reads in 228.731 CPU sec, 7.226 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285961, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (304.37, 142.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 647, 1121)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2927)
[M::mem_pestat] mean and std.dev: (722.31, 404.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3830)
[M::mem_pestat] skip orientation RR
[M::process] read 684184 sequences (100000106 bp) in 2.693 CPU sec, 2.859 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285676, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 286, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (305.17, 142.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684608 reads in 185.315 CPU sec, 5.871 real sec
[M::process] read 684018 sequences (100000281 bp) in 1.848 CPU sec, 1.876 real sec...
[M::mem_process_seqs] Processed 684358 reads in 106.099 CPU sec, 3.345 real sec
sorting block 76, #reads = 1085777 mem = 536879003 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285911, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (304.37, 141.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683976 sequences (100000267 bp) in 2.846 CPU sec, 2.987 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286050, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 286, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (305.83, 142.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683960 sequences (100000160 bp) in 1.620 CPU sec, 1.612 real sec...
[M::mem_process_seqs] Processed 684184 reads in 164.927 CPU sec, 5.231 real sec
sorting block 77, #reads = 1086838 mem = 536885973 peak = 908648448
[M::mem_process_seqs] Processed 684018 reads in 103.791 CPU sec, 3.272 real sec
sorting block 78, #reads = 1086455 mem = 536881398 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285238, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 286, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (305.54, 142.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683978 sequences (100000018 bp) in 2.790 CPU sec, 2.925 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285830, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 285, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (304.20, 141.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683976 reads in 162.338 CPU sec, 5.145 real sec
[M::process] read 684242 sequences (100000220 bp) in 2.027 CPU sec, 2.040 real sec...
[M::mem_process_seqs] Processed 683960 reads in 102.227 CPU sec, 3.214 real sec
sorting block 79, #reads = 1085325 mem = 536876654 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285732, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 283, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (302.39, 140.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 683842 sequences (100000099 bp) in 2.850 CPU sec, 2.977 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285458, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (193, 284, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (302.68, 140.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683978 reads in 161.997 CPU sec, 5.136 real sec
[M::mem_process_seqs] Processed 684242 reads in 102.585 CPU sec, 3.221 real sec
[M::process] read 684020 sequences (100000023 bp) in 2.139 CPU sec, 2.160 real sec...
sorting block 80, #reads = 1084965 mem = 536877653 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285362, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 285, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (303.98, 140.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691784 sequences (100000009 bp) in 2.776 CPU sec, 2.910 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285145, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (194, 287, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (306.11, 142.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1018)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 683842 reads in 166.717 CPU sec, 5.278 real sec
[M::process] read 692282 sequences (100000163 bp) in 2.036 CPU sec, 2.042 real sec...
sorting block 81, #reads = 1083536 mem = 536878348 peak = 908648448
[M::mem_process_seqs] Processed 684020 reads in 103.485 CPU sec, 3.257 real sec
sorting block 82, #reads = 1082859 mem = 536882103 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288230, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 249, 357)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727)
[M::mem_pestat] mean and std.dev: (273.78, 132.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 912)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691740 sequences (100000284 bp) in 2.477 CPU sec, 2.639 real sec...
[M::process] read 691308 sequences (100000010 bp) in 1.490 CPU sec, 1.493 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288920, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 249, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744)
[M::mem_pestat] mean and std.dev: (276.48, 135.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 935)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 691784 reads in 172.039 CPU sec, 5.472 real sec
[M::mem_process_seqs] Processed 692282 reads in 102.675 CPU sec, 3.262 real sec
sorting block 83, #reads = 1084924 mem = 536882493 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288410, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 251, 364)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748)
[M::mem_pestat] mean and std.dev: (278.55, 137.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 940)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690812 sequences (100000127 bp) in 2.995 CPU sec, 3.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288214, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 252, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 749)
[M::mem_pestat] mean and std.dev: (278.81, 136.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 941)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689068 sequences (100000298 bp) in 1.960 CPU sec, 1.962 real sec...
[M::mem_process_seqs] Processed 691740 reads in 171.265 CPU sec, 5.414 real sec
[M::mem_process_seqs] Processed 691308 reads in 107.514 CPU sec, 3.388 real sec
sorting block 84, #reads = 1088120 mem = 536877779 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288213, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 256, 371)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763)
[M::mem_pestat] mean and std.dev: (282.82, 138.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 959)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689052 sequences (100000028 bp) in 2.887 CPU sec, 3.040 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287588, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 262, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 779)
[M::mem_pestat] mean and std.dev: (288.45, 140.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688340 sequences (100000130 bp) in 1.820 CPU sec, 1.822 real sec...
[M::mem_process_seqs] Processed 690812 reads in 170.441 CPU sec, 5.398 real sec
[M::mem_process_seqs] Processed 689068 reads in 103.450 CPU sec, 3.271 real sec
sorting block 85, #reads = 1087554 mem = 536876947 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287336, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 264, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (290.27, 141.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 991)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688058 sequences (100000215 bp) in 2.745 CPU sec, 2.871 real sec...
[M::process] read 687658 sequences (100000198 bp) in 1.520 CPU sec, 1.519 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287318, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796)
[M::mem_pestat] mean and std.dev: (292.83, 142.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1001)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689052 reads in 167.594 CPU sec, 5.325 real sec
sorting block 86, #reads = 1087679 mem = 536877879 peak = 908648448
[M::mem_process_seqs] Processed 688340 reads in 104.141 CPU sec, 3.301 real sec
sorting block 87, #reads = 1086491 mem = 536880352 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287300, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 269, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (294.87, 143.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1010)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687356 sequences (100000236 bp) in 2.781 CPU sec, 2.915 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286788, 13, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (296.48, 144.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (662, 702, 1810)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4106)
[M::mem_pestat] mean and std.dev: (1168.54, 1083.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5502)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 687312 sequences (100000234 bp) in 2.020 CPU sec, 2.020 real sec...
[M::mem_process_seqs] Processed 688058 reads in 167.170 CPU sec, 5.294 real sec
[M::mem_process_seqs] Processed 687658 reads in 102.988 CPU sec, 3.248 real sec
sorting block 88, #reads = 1087143 mem = 536881005 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287309, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 274, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (298.07, 144.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686796 sequences (100000193 bp) in 2.936 CPU sec, 3.047 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286691, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 272, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (296.33, 143.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687356 reads in 160.733 CPU sec, 5.092 real sec
[M::process] read 686424 sequences (100000266 bp) in 2.059 CPU sec, 2.075 real sec...
[M::mem_process_seqs] Processed 687312 reads in 101.083 CPU sec, 3.178 real sec
sorting block 89, #reads = 1086988 mem = 536879748 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287325, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 276, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (300.86, 146.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1033)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687094 sequences (100000141 bp) in 2.975 CPU sec, 3.086 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286618, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822)
[M::mem_pestat] mean and std.dev: (301.77, 146.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1034)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686796 reads in 153.856 CPU sec, 4.887 real sec
[M::mem_process_seqs] Processed 686424 reads in 97.835 CPU sec, 3.076 real sec
sorting block 90, #reads = 1084835 mem = 536883848 peak = 908648448
[M::process] read 686436 sequences (100000297 bp) in 2.243 CPU sec, 2.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286828, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 275, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826)
[M::mem_pestat] mean and std.dev: (301.40, 147.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1040)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685886 sequences (100000200 bp) in 2.512 CPU sec, 2.664 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286591, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 276, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 816)
[M::mem_pestat] mean and std.dev: (300.56, 145.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1026)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687094 reads in 158.006 CPU sec, 5.016 real sec
sorting block 91, #reads = 1084255 mem = 536876803 peak = 908648448
[M::mem_process_seqs] Processed 686436 reads in 102.270 CPU sec, 3.218 real sec
[M::process] read 685910 sequences (100000195 bp) in 2.052 CPU sec, 2.083 real sec...
sorting block 92, #reads = 1085050 mem = 536879400 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286554, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 276, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (299.48, 142.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685660 sequences (100000180 bp) in 2.698 CPU sec, 2.837 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286494, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 277, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (301.09, 144.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685886 reads in 159.030 CPU sec, 5.054 real sec
[M::process] read 685188 sequences (100000252 bp) in 1.590 CPU sec, 1.605 real sec...
[M::mem_process_seqs] Processed 685910 reads in 99.925 CPU sec, 3.156 real sec
sorting block 93, #reads = 1083370 mem = 536880873 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286489, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 278, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (301.30, 144.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685348 sequences (100000128 bp) in 2.976 CPU sec, 3.106 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285659, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810)
[M::mem_pestat] mean and std.dev: (301.71, 143.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685660 reads in 155.917 CPU sec, 4.941 real sec
[M::mem_process_seqs] Processed 685188 reads in 99.812 CPU sec, 3.135 real sec
[M::process] read 685464 sequences (100000184 bp) in 2.305 CPU sec, 2.364 real sec...
sorting block 94, #reads = 1084522 mem = 536878675 peak = 908648448
[M::process] read 685540 sequences (100000128 bp) in 2.372 CPU sec, 2.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286019, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (300.54, 143.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1012)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685464 reads in 77.156 CPU sec, 2.430 real sec
sorting block 95, #reads = 1084289 mem = 536878690 peak = 908648448
[M::process] read 685718 sequences (100000283 bp) in 1.764 CPU sec, 1.793 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286932, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 277, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (301.62, 144.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285891, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (299.11, 142.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685348 reads in 218.457 CPU sec, 6.943 real sec
[M::mem_process_seqs] Processed 685540 reads in 102.780 CPU sec, 3.255 real sec
[M::process] read 686112 sequences (100000041 bp) in 1.811 CPU sec, 1.907 real sec...
sorting block 96, #reads = 1084269 mem = 536878029 peak = 908648448
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286003, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (298.80, 142.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685728 sequences (100000193 bp) in 2.620 CPU sec, 2.728 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286392, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 274, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (298.10, 142.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685718 reads in 143.968 CPU sec, 4.586 real sec
[M::mem_process_seqs] Processed 686112 reads in 92.418 CPU sec, 2.911 real sec
sorting block 97, #reads = 1083236 mem = 536879285 peak = 908648448
[M::process] read 685504 sequences (100000301 bp) in 2.068 CPU sec, 2.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286137, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 391)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (298.88, 142.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685352 sequences (100000267 bp) in 2.195 CPU sec, 2.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286201, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803)
[M::mem_pestat] mean and std.dev: (299.92, 142.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685728 reads in 141.562 CPU sec, 4.497 real sec
sorting block 98, #reads = 1083925 mem = 536877189 peak = 908648448
[M::process] read 685372 sequences (100000068 bp) in 1.444 CPU sec, 1.445 real sec...
[M::mem_process_seqs] Processed 685504 reads in 87.027 CPU sec, 2.746 real sec
sorting block 99, #reads = 1083045 mem = 536880277 peak = 916574208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286177, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (299.68, 142.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685222 sequences (100000069 bp) in 2.383 CPU sec, 2.529 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286009, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 277, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (300.23, 142.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687514 sequences (100000139 bp) in 1.480 CPU sec, 1.486 real sec...
[M::mem_process_seqs] Processed 685352 reads in 137.643 CPU sec, 4.365 real sec
[M::mem_process_seqs] Processed 685372 reads in 87.560 CPU sec, 2.764 real sec
sorting block 100, #reads = 1083403 mem = 536879529 peak = 916574208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286054, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804)
[M::mem_pestat] mean and std.dev: (300.85, 142.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1009)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 694524 sequences (100000257 bp) in 2.145 CPU sec, 2.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286800, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (293.06, 141.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 693840 sequences (100000002 bp) in 1.550 CPU sec, 1.546 real sec...
[M::mem_process_seqs] Processed 685222 reads in 147.903 CPU sec, 4.699 real sec
[M::mem_process_seqs] Processed 687514 reads in 97.540 CPU sec, 3.084 real sec
sorting block 101, #reads = 1082577 mem = 536878379 peak = 916574208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 290147, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 241, 346)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704)
[M::mem_pestat] mean and std.dev: (265.81, 127.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 883)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 693176 sequences (100000233 bp) in 2.613 CPU sec, 2.763 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 289205, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 243, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714)
[M::mem_pestat] mean and std.dev: (268.58, 129.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 896)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 694524 reads in 158.731 CPU sec, 5.019 real sec
[M::process] read 693108 sequences (100000055 bp) in 2.040 CPU sec, 2.035 real sec...
sorting block 102, #reads = 1080533 mem = 536880619 peak = 916574208
[M::mem_process_seqs] Processed 693840 reads in 99.912 CPU sec, 3.149 real sec
sorting block 103, #reads = 1084371 mem = 536878465 peak = 916574208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289224, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 247, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (272.69, 132.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691738 sequences (100000182 bp) in 2.662 CPU sec, 2.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 289431, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 246, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (271.09, 130.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 909)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 693176 reads in 159.583 CPU sec, 5.052 real sec
[M::process] read 691118 sequences (100000029 bp) in 2.106 CPU sec, 2.132 real sec...
[M::mem_process_seqs] Processed 693108 reads in 102.219 CPU sec, 3.216 real sec
sorting block 104, #reads = 1083459 mem = 536880063 peak = 916574208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288455, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 251, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (275.38, 132.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 924)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690838 sequences (100000254 bp) in 2.901 CPU sec, 3.020 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288504, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 255, 366)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 750)
[M::mem_pestat] mean and std.dev: (279.05, 133.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 942)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 691738 reads in 159.586 CPU sec, 5.059 real sec
[M::mem_process_seqs] Processed 691118 reads in 102.718 CPU sec, 3.227 real sec
[M::process] read 690384 sequences (100000110 bp) in 2.174 CPU sec, 2.199 real sec...
sorting block 105, #reads = 1081582 mem = 536879123 peak = 916574208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 288212, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 255, 369)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757)
[M::mem_pestat] mean and std.dev: (280.71, 135.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 951)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689410 sequences (100000265 bp) in 2.300 CPU sec, 2.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287983, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 257, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766)
[M::mem_pestat] mean and std.dev: (283.03, 137.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 963)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688416 sequences (100000290 bp) in 1.520 CPU sec, 1.518 real sec...
[M::mem_process_seqs] Processed 690838 reads in 156.512 CPU sec, 4.980 real sec
sorting block 106, #reads = 1081771 mem = 536881335 peak = 916574208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287736, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 261, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (286.14, 137.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688344 sequences (100000008 bp) in 2.187 CPU sec, 2.190 real sec...
[M::mem_process_seqs] Processed 689410 reads in 86.456 CPU sec, 2.920 real sec
[M::mem_process_seqs] Processed 690384 reads in 208.074 CPU sec, 6.785 real sec
sorting block 107, #reads = 1083177 mem = 536879787 peak = 916574208
[M::process] read 688148 sequences (100000134 bp) in 2.656 CPU sec, 2.804 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287203, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 266, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784)
[M::mem_pestat] mean and std.dev: (290.41, 139.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 985)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688344 reads in 80.342 CPU sec, 2.533 real sec
sorting block 108, #reads = 1082791 mem = 536877978 peak = 916574208
[M::process] read 687942 sequences (100000027 bp) in 2.712 CPU sec, 2.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287194, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 265, 379)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777)
[M::mem_pestat] mean and std.dev: (288.33, 137.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 976)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286670, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 266, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (290.24, 139.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688416 reads in 242.711 CPU sec, 7.681 real sec
[M::mem_process_seqs] Processed 688148 reads in 108.182 CPU sec, 3.418 real sec
[M::process] read 688142 sequences (100000014 bp) in 2.256 CPU sec, 2.401 real sec...
sorting block 109, #reads = 1082908 mem = 536878890 peak = 916574208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287779, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 269, 386)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794)
[M::mem_pestat] mean and std.dev: (293.11, 141.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687422 sequences (100000011 bp) in 2.253 CPU sec, 2.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287543, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 266, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790)
[M::mem_pestat] mean and std.dev: (291.85, 141.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 993)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687942 reads in 143.603 CPU sec, 4.567 real sec
[M::mem_process_seqs] Processed 688142 reads in 91.121 CPU sec, 2.873 real sec
[M::process] read 687706 sequences (100000258 bp) in 2.132 CPU sec, 2.267 real sec...
sorting block 110, #reads = 1084481 mem = 536883354 peak = 916574208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286949, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 271, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (295.92, 143.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686388 sequences (100000283 bp) in 2.179 CPU sec, 2.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287323, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 269, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (292.57, 140.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687422 reads in 148.807 CPU sec, 4.743 real sec
[M::mem_process_seqs] Processed 687706 reads in 94.940 CPU sec, 2.989 real sec
[M::process] read 686400 sequences (100000060 bp) in 1.679 CPU sec, 1.734 real sec...
sorting block 111, #reads = 1084379 mem = 536878069 peak = 916574208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286308, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (297.34, 141.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 112, #reads = 1084438 mem = 536878698 peak = 916574208
[M::process] read 685860 sequences (100000156 bp) in 3.125 CPU sec, 3.310 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 287087, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (298.10, 143.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686388 reads in 145.256 CPU sec, 4.628 real sec
[M::mem_process_seqs] Processed 686400 reads in 90.619 CPU sec, 2.860 real sec
[M::process] read 685856 sequences (100000183 bp) in 2.103 CPU sec, 2.245 real sec...
sorting block 113, #reads = 1083860 mem = 536878622 peak = 916574208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286477, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 277, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (300.38, 144.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685480 sequences (100000216 bp) in 1.834 CPU sec, 1.937 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 286347, 3, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (307, 307, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (45, 700)
[M::mem_pestat] mean and std.dev: (339.11, 75.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (299.25, 143.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1007)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 685860 reads in 137.869 CPU sec, 4.384 real sec
[M::process] read 685760 sequences (100000277 bp) in 1.471 CPU sec, 1.484 real sec...
[M::mem_process_seqs] Processed 685856 reads in 87.435 CPU sec, 2.754 real sec
sorting block 114, #reads = 1083009 mem = 536879775 peak = 916574208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285929, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (301.56, 144.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1016)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685842 sequences (100000228 bp) in 2.258 CPU sec, 2.453 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286494, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (300.06, 144.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685480 reads in 135.321 CPU sec, 4.308 real sec
[M::mem_process_seqs] Processed 685760 reads in 84.684 CPU sec, 2.669 real sec
[M::process] read 685970 sequences (100000007 bp) in 1.595 CPU sec, 1.622 real sec...
sorting block 115, #reads = 1082650 mem = 536879507 peak = 916574208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286338, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (300.62, 145.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685560 sequences (100000113 bp) in 1.911 CPU sec, 2.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286056, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 275, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (300.92, 145.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686274 sequences (100000260 bp) in 1.400 CPU sec, 1.407 real sec...
[M::mem_process_seqs] Processed 685842 reads in 135.754 CPU sec, 4.323 real sec
sorting block 116, #reads = 1084702 mem = 536884466 peak = 916574208
[M::mem_process_seqs] Processed 685970 reads in 85.341 CPU sec, 2.708 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286158, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 276, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817)
[M::mem_pestat] mean and std.dev: (301.97, 145.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1027)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 117, #reads = 1083744 mem = 536877581 peak = 916574208
[M::process] read 685656 sequences (100000095 bp) in 2.435 CPU sec, 2.688 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286331, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (299.21, 145.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685560 reads in 140.374 CPU sec, 4.461 real sec
[M::process] read 685528 sequences (100000111 bp) in 1.609 CPU sec, 1.631 real sec...
[M::mem_process_seqs] Processed 686274 reads in 90.299 CPU sec, 2.847 real sec
sorting block 118, #reads = 1083229 mem = 536878281 peak = 916574208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286115, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 276, 398)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820)
[M::mem_pestat] mean and std.dev: (302.46, 146.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688850 sequences (100000080 bp) in 2.024 CPU sec, 2.127 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286051, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 277, 399)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (303.53, 147.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1032)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 691680 sequences (100000053 bp) in 1.459 CPU sec, 1.457 real sec...
[M::mem_process_seqs] Processed 685656 reads in 139.302 CPU sec, 4.429 real sec
[M::mem_process_seqs] Processed 685528 reads in 89.462 CPU sec, 2.830 real sec
sorting block 119, #reads = 1082808 mem = 536876893 peak = 916574208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286894, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 263, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (288.78, 140.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 692242 sequences (100000226 bp) in 2.755 CPU sec, 2.946 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287916, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 252, 361)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737)
[M::mem_pestat] mean and std.dev: (276.55, 134.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688850 reads in 153.267 CPU sec, 4.860 real sec
[M::process] read 691722 sequences (100000298 bp) in 1.689 CPU sec, 1.685 real sec...
[M::mem_process_seqs] Processed 691680 reads in 90.434 CPU sec, 2.856 real sec
sorting block 120, #reads = 1077872 mem = 536878232 peak = 916574208
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288330, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (171, 249, 359)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (275.41, 134.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 923)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690708 sequences (100000186 bp) in 2.077 CPU sec, 2.208 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288200, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (172, 251, 362)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742)
[M::mem_pestat] mean and std.dev: (277.25, 135.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 932)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690214 sequences (100000276 bp) in 1.480 CPU sec, 1.481 real sec...
[M::mem_process_seqs] Processed 692242 reads in 137.776 CPU sec, 4.378 real sec
sorting block 121, #reads = 1082259 mem = 536880134 peak = 916574208
[M::mem_process_seqs] Processed 691722 reads in 85.570 CPU sec, 2.706 real sec
sorting block 122, #reads = 1087213 mem = 536878926 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288192, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (175, 255, 368)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754)
[M::mem_pestat] mean and std.dev: (280.85, 136.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 947)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689880 sequences (100000071 bp) in 2.344 CPU sec, 2.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287627, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 258, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764)
[M::mem_pestat] mean and std.dev: (283.75, 138.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 960)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690708 reads in 134.600 CPU sec, 4.269 real sec
[M::process] read 689266 sequences (100000200 bp) in 1.480 CPU sec, 1.474 real sec...
[M::mem_process_seqs] Processed 690214 reads in 83.375 CPU sec, 2.638 real sec
sorting block 123, #reads = 1087145 mem = 536878683 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287634, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 259, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 767)
[M::mem_pestat] mean and std.dev: (284.94, 139.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 964)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688310 sequences (100000103 bp) in 2.056 CPU sec, 2.196 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287480, 2, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 263, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (287.88, 139.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 689880 reads in 130.607 CPU sec, 4.154 real sec
[M::process] read 688662 sequences (100000288 bp) in 1.501 CPU sec, 1.502 real sec...
[M::mem_process_seqs] Processed 689266 reads in 81.456 CPU sec, 2.571 real sec
sorting block 124, #reads = 1087974 mem = 536880405 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286841, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778)
[M::mem_pestat] mean and std.dev: (290.18, 139.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 977)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687810 sequences (100000090 bp) in 2.549 CPU sec, 2.745 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 287754, 1, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (587, 2239, 2239)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5543)
[M::mem_pestat] mean and std.dev: (1394.30, 873.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7195)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 264, 378)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774)
[M::mem_pestat] mean and std.dev: (288.66, 139.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 972)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 688310 reads in 128.772 CPU sec, 4.101 real sec
[M::mem_process_seqs] Processed 688662 reads in 80.928 CPU sec, 2.557 real sec
sorting block 125, #reads = 1087799 mem = 536886713 peak = 945311744
[M::process] read 688098 sequences (100000298 bp) in 1.775 CPU sec, 1.863 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287108, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 268, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (291.97, 140.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 986)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 126, #reads = 1087750 mem = 536881831 peak = 945311744
[M::process] read 687684 sequences (100000104 bp) in 1.959 CPU sec, 2.108 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 287503, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 781)
[M::mem_pestat] mean and std.dev: (290.85, 139.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 981)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687810 reads in 129.404 CPU sec, 4.123 real sec
[M::mem_process_seqs] Processed 688098 reads in 81.560 CPU sec, 2.572 real sec
[M::process] read 687720 sequences (100000055 bp) in 1.731 CPU sec, 1.792 real sec...
sorting block 127, #reads = 1087840 mem = 536883705 peak = 945311744
[M::process] read 687138 sequences (100000024 bp) in 2.028 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286759, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 268, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (290.69, 138.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687720 reads in 72.866 CPU sec, 2.298 real sec
[M::process] read 687142 sequences (100000121 bp) in 1.589 CPU sec, 1.639 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286921, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 269, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (292.12, 139.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287028, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (293.05, 139.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687684 reads in 202.458 CPU sec, 6.440 real sec
sorting block 128, #reads = 1087267 mem = 536884215 peak = 945311744
[M::mem_process_seqs] Processed 687138 reads in 93.597 CPU sec, 2.967 real sec
[M::process] read 687656 sequences (100000156 bp) in 1.859 CPU sec, 1.928 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286984, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 270, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783)
[M::mem_pestat] mean and std.dev: (293.09, 139.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 983)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 129, #reads = 1085992 mem = 536880099 peak = 945311744
[M::process] read 688136 sequences (100000129 bp) in 2.038 CPU sec, 2.189 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287280, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (181, 267, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787)
[M::mem_pestat] mean and std.dev: (292.09, 140.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 989)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 687142 reads in 133.212 CPU sec, 4.244 real sec
[M::mem_process_seqs] Processed 687656 reads in 85.720 CPU sec, 2.704 real sec
[M::process] read 688468 sequences (100000147 bp) in 1.611 CPU sec, 1.644 real sec...
sorting block 130, #reads = 1086071 mem = 536878432 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 287499, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 267, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782)
[M::mem_pestat] mean and std.dev: (291.43, 140.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 982)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688374 sequences (100000262 bp) in 2.366 CPU sec, 2.541 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286980, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 262, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759)
[M::mem_pestat] mean and std.dev: (285.85, 136.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688136 reads in 134.869 CPU sec, 4.285 real sec
[M::process] read 688404 sequences (100000080 bp) in 1.503 CPU sec, 1.514 real sec...
[M::mem_process_seqs] Processed 688468 reads in 84.991 CPU sec, 2.684 real sec
sorting block 131, #reads = 1085527 mem = 536878605 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287114, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 264, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771)
[M::mem_pestat] mean and std.dev: (288.10, 138.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 968)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687820 sequences (100000281 bp) in 1.991 CPU sec, 2.114 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287184, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 263, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 770)
[M::mem_pestat] mean and std.dev: (287.89, 139.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 967)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688374 reads in 135.469 CPU sec, 4.316 real sec
[M::process] read 687200 sequences (100000074 bp) in 1.450 CPU sec, 1.441 real sec...
sorting block 132, #reads = 1086709 mem = 536883504 peak = 945311744
[M::mem_process_seqs] Processed 688404 reads in 84.817 CPU sec, 2.683 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 287106, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 267, 384)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788)
[M::mem_pestat] mean and std.dev: (292.89, 142.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 990)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 133, #reads = 1085792 mem = 536884222 peak = 945311744
[M::process] read 686844 sequences (100000112 bp) in 2.313 CPU sec, 2.433 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287247, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 271, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (296.76, 144.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686490 sequences (100000005 bp) in 1.460 CPU sec, 1.464 real sec...
[M::mem_process_seqs] Processed 687820 reads in 135.239 CPU sec, 4.288 real sec
[M::mem_process_seqs] Processed 687200 reads in 84.560 CPU sec, 2.670 real sec
sorting block 134, #reads = 1085219 mem = 536877607 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287216, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 272, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (297.22, 144.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1008)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686328 sequences (100000145 bp) in 2.166 CPU sec, 2.364 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286926, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 392)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (298.49, 144.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1013)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686844 reads in 135.473 CPU sec, 4.318 real sec
[M::mem_process_seqs] Processed 686490 reads in 85.896 CPU sec, 2.706 real sec
[M::process] read 686168 sequences (100000070 bp) in 1.605 CPU sec, 1.637 real sec...
sorting block 135, #reads = 1084183 mem = 536878517 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286972, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 275, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (299.79, 144.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 685982 sequences (100000182 bp) in 2.222 CPU sec, 2.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286726, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (186, 275, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807)
[M::mem_pestat] mean and std.dev: (299.83, 144.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1014)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686328 reads in 135.140 CPU sec, 4.290 real sec
[M::process] read 687088 sequences (100000129 bp) in 1.490 CPU sec, 1.484 real sec...
[M::mem_process_seqs] Processed 686168 reads in 85.535 CPU sec, 2.703 real sec
sorting block 136, #reads = 1084599 mem = 536877307 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286219, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 276, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808)
[M::mem_pestat] mean and std.dev: (300.52, 145.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1015)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (135, 237, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (241.80, 79.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 859)
[M::mem_pestat] skip orientation RR
[M::process] read 686438 sequences (100000285 bp) in 2.248 CPU sec, 2.403 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286758, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 267, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780)
[M::mem_pestat] mean and std.dev: (292.67, 141.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 979)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685982 reads in 139.786 CPU sec, 4.438 real sec
[M::process] read 687376 sequences (100000214 bp) in 1.520 CPU sec, 1.518 real sec...
sorting block 137, #reads = 1084703 mem = 536882192 peak = 945311744
[M::mem_process_seqs] Processed 687088 reads in 88.649 CPU sec, 2.797 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286485, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 272, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (297.58, 144.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 138, #reads = 1082241 mem = 536878420 peak = 945311744
[M::process] read 694838 sequences (100000245 bp) in 2.128 CPU sec, 2.259 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286780, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 268, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798)
[M::mem_pestat] mean and std.dev: (295.13, 144.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1003)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 694310 sequences (100000186 bp) in 1.490 CPU sec, 1.490 real sec...
[M::mem_process_seqs] Processed 686438 reads in 150.667 CPU sec, 4.806 real sec
[M::mem_process_seqs] Processed 687376 reads in 99.836 CPU sec, 3.157 real sec
sorting block 139, #reads = 1080396 mem = 536879341 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289677, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (166, 241, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703)
[M::mem_pestat] mean and std.dev: (265.65, 128.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 882)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 694308 sequences (100000237 bp) in 2.064 CPU sec, 2.169 real sec...
[M::process] read 694652 sequences (100000115 bp) in 1.530 CPU sec, 1.522 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 289164, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 242, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711)
[M::mem_pestat] mean and std.dev: (267.66, 129.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 892)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 694838 reads in 150.936 CPU sec, 4.804 real sec
[M::mem_process_seqs] Processed 694310 reads in 93.257 CPU sec, 2.951 real sec
sorting block 140, #reads = 1077775 mem = 536877479 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289700, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 242, 349)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713)
[M::mem_pestat] mean and std.dev: (267.56, 129.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 895)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 693816 sequences (100000102 bp) in 2.125 CPU sec, 2.254 real sec...
[M::process] read 693474 sequences (100000245 bp) in 1.500 CPU sec, 1.500 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 289465, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (167, 241, 347)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707)
[M::mem_pestat] mean and std.dev: (266.08, 127.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 887)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 694308 reads in 150.893 CPU sec, 4.798 real sec
sorting block 141, #reads = 1085410 mem = 536880003 peak = 945311744
[M::mem_process_seqs] Processed 694652 reads in 96.003 CPU sec, 3.040 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 289692, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (168, 244, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 717)
[M::mem_pestat] mean and std.dev: (269.23, 129.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 900)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 142, #reads = 1082238 mem = 536878385 peak = 945311744
[M::process] read 693036 sequences (100000293 bp) in 2.172 CPU sec, 2.298 real sec...
[M::process] read 691038 sequences (100000018 bp) in 1.500 CPU sec, 1.490 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 288874, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 245, 354)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724)
[M::mem_pestat] mean and std.dev: (270.81, 131.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 909)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 693816 reads in 148.797 CPU sec, 4.729 real sec
[M::mem_process_seqs] Processed 693474 reads in 94.280 CPU sec, 2.983 real sec
sorting block 143, #reads = 1084355 mem = 536877524 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288922, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 247, 356)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728)
[M::mem_pestat] mean and std.dev: (271.82, 130.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 914)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 690204 sequences (100000058 bp) in 2.150 CPU sec, 2.271 real sec...
[M::process] read 690042 sequences (100000196 bp) in 1.460 CPU sec, 1.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 288594, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (174, 255, 365)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747)
[M::mem_pestat] mean and std.dev: (278.66, 133.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 938)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 693036 reads in 148.508 CPU sec, 4.722 real sec
[M::mem_process_seqs] Processed 691038 reads in 90.950 CPU sec, 2.882 real sec
sorting block 144, #reads = 1080941 mem = 536878482 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 288118, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 258, 370)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758)
[M::mem_pestat] mean and std.dev: (282.18, 135.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 952)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 689592 sequences (100000289 bp) in 2.296 CPU sec, 2.481 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 288253, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (177, 259, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765)
[M::mem_pestat] mean and std.dev: (284.51, 137.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 961)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 690204 reads in 140.942 CPU sec, 4.479 real sec
[M::process] read 689396 sequences (100000243 bp) in 1.537 CPU sec, 1.543 real sec...
[M::mem_process_seqs] Processed 690042 reads in 88.291 CPU sec, 2.784 real sec
sorting block 145, #reads = 1084327 mem = 536884484 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287266, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 261, 376)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772)
[M::mem_pestat] mean and std.dev: (286.01, 137.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 970)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688994 sequences (100000297 bp) in 2.199 CPU sec, 2.331 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287386, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (178, 262, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775)
[M::mem_pestat] mean and std.dev: (287.32, 139.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 974)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 688756 sequences (100000052 bp) in 1.450 CPU sec, 1.448 real sec...
[M::mem_process_seqs] Processed 689592 reads in 139.468 CPU sec, 4.434 real sec
sorting block 146, #reads = 1084669 mem = 536878173 peak = 945311744
[M::mem_process_seqs] Processed 689396 reads in 87.787 CPU sec, 2.775 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 287568, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 264, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773)
[M::mem_pestat] mean and std.dev: (287.93, 138.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 971)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 147, #reads = 1084171 mem = 536880962 peak = 945311744
[M::process] read 688582 sequences (100000042 bp) in 2.161 CPU sec, 2.272 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287591, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 264, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (289.78, 140.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688994 reads in 137.345 CPU sec, 4.369 real sec
[M::process] read 688228 sequences (100000046 bp) in 1.494 CPU sec, 1.505 real sec...
[M::mem_process_seqs] Processed 688756 reads in 85.978 CPU sec, 2.710 real sec
sorting block 148, #reads = 1085304 mem = 536880394 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287207, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (179, 264, 381)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785)
[M::mem_pestat] mean and std.dev: (290.10, 141.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 987)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687710 sequences (100000249 bp) in 2.470 CPU sec, 2.580 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 287082, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (180, 266, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786)
[M::mem_pestat] mean and std.dev: (291.48, 141.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 988)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 688582 reads in 138.579 CPU sec, 4.403 real sec
[M::process] read 687810 sequences (100000273 bp) in 1.497 CPU sec, 1.501 real sec...
[M::mem_process_seqs] Processed 688228 reads in 86.834 CPU sec, 2.736 real sec
sorting block 149, #reads = 1084518 mem = 536888267 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 287049, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 268, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797)
[M::mem_pestat] mean and std.dev: (294.37, 143.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1002)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687210 sequences (100000195 bp) in 2.114 CPU sec, 2.237 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287288, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (182, 270, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 800)
[M::mem_pestat] mean and std.dev: (295.03, 143.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1006)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 687048 sequences (100000029 bp) in 1.440 CPU sec, 1.439 real sec...
[M::mem_process_seqs] Processed 687710 reads in 135.906 CPU sec, 4.320 real sec
[M::mem_process_seqs] Processed 687810 reads in 84.809 CPU sec, 2.677 real sec
sorting block 150, #reads = 1084351 mem = 536880548 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 286960, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 271, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793)
[M::mem_pestat] mean and std.dev: (294.99, 141.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 996)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686820 sequences (100000135 bp) in 2.435 CPU sec, 2.557 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286499, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 273, 389)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799)
[M::mem_pestat] mean and std.dev: (296.22, 142.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1004)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 686200 sequences (100000018 bp) in 1.420 CPU sec, 1.421 real sec...
[M::mem_process_seqs] Processed 687210 reads in 135.526 CPU sec, 4.297 real sec
sorting block 151, #reads = 1085404 mem = 536879771 peak = 945311744
[M::mem_process_seqs] Processed 687048 reads in 84.506 CPU sec, 2.669 real sec
sorting block 152, #reads = 1083645 mem = 536879516 peak = 945311744
[M::process] read 685842 sequences (100000179 bp) in 2.140 CPU sec, 2.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286502, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 277, 395)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 811)
[M::mem_pestat] mean and std.dev: (300.66, 144.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1019)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686200 reads in 72.983 CPU sec, 2.296 real sec
[M::process] read 686044 sequences (100000140 bp) in 1.445 CPU sec, 1.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286742, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 274, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (298.73, 144.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286453, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 280, 397)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (302.63, 145.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1024)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686820 reads in 205.038 CPU sec, 6.511 real sec
[M::process] read 685744 sequences (100000193 bp) in 1.804 CPU sec, 1.820 real sec...
sorting block 153, #reads = 1084954 mem = 536879596 peak = 945311744
[M::mem_process_seqs] Processed 685842 reads in 95.876 CPU sec, 3.037 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286466, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (188, 279, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812)
[M::mem_pestat] mean and std.dev: (301.77, 144.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1020)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 154, #reads = 1085232 mem = 536879341 peak = 945311744
[M::process] read 685916 sequences (100000123 bp) in 2.523 CPU sec, 2.687 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286321, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 277, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (299.83, 142.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 686044 reads in 132.280 CPU sec, 4.206 real sec
[M::mem_process_seqs] Processed 685744 reads in 84.052 CPU sec, 2.651 real sec
[M::process] read 684838 sequences (100000170 bp) in 1.635 CPU sec, 1.697 real sec...
sorting block 155, #reads = 1084938 mem = 536881011 peak = 945311744
[M::process] read 685120 sequences (100000085 bp) in 2.256 CPU sec, 2.487 real sec...
[M::process] read 174374 sequences (25373526 bp) in 0.580 CPU sec, 0.583 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 285921, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 282, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 806)
[M::mem_pestat] mean and std.dev: (302.65, 142.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 684838 reads in 71.782 CPU sec, 2.294 real sec
sorting block 156, #reads = 1085145 mem = 536882259 peak = 945311744
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286278, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (187, 277, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805)
[M::mem_pestat] mean and std.dev: (299.69, 142.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1011)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285603, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 278, 393)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801)
[M::mem_pestat] mean and std.dev: (300.44, 142.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1005)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 685916 reads in 203.782 CPU sec, 6.551 real sec
[M::mem_process_seqs] Processed 685120 reads in 95.213 CPU sec, 3.092 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 72768, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 270, 382)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 776)
[M::mem_pestat] mean and std.dev: (292.74, 138.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 973)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 174374 reads in 22.002 CPU sec, 0.745 real sec
sorting block 157, #reads = 1083391 mem = 536879504 peak = 945311744
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 171235314
overall: 69646692352 mem 23740.497 user 964.286 sys 821.109 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_cfy-T1-TDNA-1_A23MHWWLT4_1.L006\tPU:L006\tSM:659_cfy-T1-TDNA-1_A23MHWWLT4_1\tLB:659_cfy-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_cfy-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_cfy-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 821.140 sec; CPU: 24704.787 sec
sorting block 158, #reads = 536346 mem = 266731953 peak = 945311744
uniq header count 1
merging 159 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 384
algo: util-sort
license: sentieon:util=1
output file size: 9353647085
output reads: 172087199
bam_mem_sort: 159 calls 91.080 user 1.103 sys 92.470 real
bam_write: 159 calls 48.886 user 3.347 sys 55.313 real
execute: 1 calls 33.276 user 2.566 sys 874.315 real
merge_files: 1 calls 33.273 user 2.499 sys 52.472 real
parse_chunk: 2536 calls 112.653 user 28.529 sys 144.032 real
read_chunk: 6839 calls 64.247 user 53.723 sys 716.375 real
sort_block: 1 calls 49.610 user 52.922 sys 694.041 real
write_chunk: 4301 calls 32.911 user 2.115 sys 51.466 real
overall: 3057242112 mem 2149.622 user 107.742 sys 874.335 real
ls: cannot access '*.cram': No such file or directory