cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
No more license available for sentieon:klib
version: sentieon-genomics-202308.03
No more license available for sentieon:klib
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
No more license available for sentieon:klib
[M::main_mem] bwa index loaded in 1708.843 CPU sec, 659.226 real sec
[M::process] read 702102 sequences (100000222 bp) in 0.951 CPU sec, 0.957 real sec...
[M::process] read 701864 sequences (100000170 bp) in 1.229 CPU sec, 1.253 real sec...
[M::process] read 701236 sequences (100000094 bp) in 1.244 CPU sec, 1.262 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292033, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624)
[M::mem_pestat] mean and std.dev: (239.17, 113.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 781)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701390 sequences (100000059 bp) in 1.193 CPU sec, 1.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 292142, 7, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 329, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133)
[M::mem_pestat] mean and std.dev: (374.56, 261.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1442)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627)
[M::mem_pestat] mean and std.dev: (240.19, 113.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 785)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 291442, 5, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 457, 769)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1857)
[M::mem_pestat] mean and std.dev: (385.88, 223.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2401)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (241.62, 114.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 702102 reads in 215.990 CPU sec, 6.912 real sec
[M::mem_process_seqs] Processed 701864 reads in 139.255 CPU sec, 4.489 real sec
[M::mem_process_seqs] Processed 701236 reads in 150.706 CPU sec, 4.877 real sec
sorting block 0, #reads = 1096325 mem = 536877418 peak = 589606912
[M::process] read 701624 sequences (100000103 bp) in 1.855 CPU sec, 2.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292168, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.72, 115.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699498 sequences (100000259 bp) in 1.288 CPU sec, 1.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292256, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (240.95, 114.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 793)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701390 reads in 130.355 CPU sec, 4.146 real sec
sorting block 1, #reads = 1095084 mem = 536886824 peak = 893321216
[M::mem_process_seqs] Processed 701624 reads in 82.316 CPU sec, 2.592 real sec
[M::process] read 698866 sequences (100000211 bp) in 1.307 CPU sec, 1.342 real sec...
sorting block 2, #reads = 1095673 mem = 536880446 peak = 900374528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291394, 5, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (235, 239, 474)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (326.50, 167.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.09, 119.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698334 sequences (100000255 bp) in 1.765 CPU sec, 1.847 real sec...
[M::process] read 697980 sequences (100000290 bp) in 1.210 CPU sec, 1.215 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291048, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.96, 121.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699498 reads in 129.399 CPU sec, 4.130 real sec
[M::mem_process_seqs] Processed 698866 reads in 81.312 CPU sec, 2.592 real sec
sorting block 3, #reads = 1093790 mem = 536878607 peak = 900374528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291040, 9, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (154, 234, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421)
[M::mem_pestat] mean and std.dev: (208.89, 56.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 510)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.48, 121.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698034 sequences (100000259 bp) in 1.864 CPU sec, 1.971 real sec...
[M::process] read 696086 sequences (100000232 bp) in 1.230 CPU sec, 1.222 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291234, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.84, 121.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698334 reads in 129.837 CPU sec, 4.138 real sec
[M::mem_process_seqs] Processed 697980 reads in 81.394 CPU sec, 2.589 real sec
sorting block 4, #reads = 1094474 mem = 536877667 peak = 900374528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290836, 14, 15)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 663, 1270)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3386)
[M::mem_pestat] mean and std.dev: (815.10, 735.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4444)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.30, 118.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 901, 1909)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5467)
[M::mem_pestat] mean and std.dev: (854.57, 804.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7246)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 235, 388)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762)
[M::mem_pestat] mean and std.dev: (264.60, 110.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 949)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 696256 sequences (100000170 bp) in 1.834 CPU sec, 1.936 real sec...
[M::process] read 695454 sequences (100000108 bp) in 1.270 CPU sec, 1.266 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290307, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.44, 121.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698034 reads in 130.902 CPU sec, 4.172 real sec
sorting block 5, #reads = 1093503 mem = 536878061 peak = 900374528
[M::mem_process_seqs] Processed 696086 reads in 81.858 CPU sec, 2.612 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290338, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (255.33, 121.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 6, #reads = 1091764 mem = 536878482 peak = 900374528
[M::process] read 696062 sequences (100000072 bp) in 2.085 CPU sec, 2.244 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290079, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 234, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (256.70, 121.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695956 sequences (100000041 bp) in 1.310 CPU sec, 1.307 real sec...
[M::mem_process_seqs] Processed 696256 reads in 131.241 CPU sec, 4.181 real sec
[M::mem_process_seqs] Processed 695454 reads in 82.425 CPU sec, 2.617 real sec
sorting block 7, #reads = 1093315 mem = 536877782 peak = 900374528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290447, 8, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (152, 222, 697)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1787)
[M::mem_pestat] mean and std.dev: (441.17, 437.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2332)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (254.06, 120.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 695734 sequences (100000110 bp) in 1.993 CPU sec, 2.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 289831, 6, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (136, 151, 227)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409)
[M::mem_pestat] mean and std.dev: (155.00, 42.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 500)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (253.95, 119.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 241, 394)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (241.91, 109.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 695180 sequences (100000232 bp) in 1.320 CPU sec, 1.326 real sec...
[M::mem_process_seqs] Processed 696062 reads in 132.250 CPU sec, 4.218 real sec
[M::mem_process_seqs] Processed 695956 reads in 82.982 CPU sec, 2.634 real sec
sorting block 8, #reads = 1092233 mem = 536883981 peak = 900374528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 289855, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 220, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751)
[M::mem_pestat] mean and std.dev: (234.92, 125.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 958)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (254.60, 120.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 695030 sequences (100000005 bp) in 2.213 CPU sec, 2.376 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289824, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 235, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (256.76, 120.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 182, 230)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404)
[M::mem_pestat] mean and std.dev: (185.27, 62.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 491)
[M::mem_pestat] skip orientation RR
[M::process] read 701486 sequences (100000076 bp) in 1.360 CPU sec, 1.360 real sec...
[M::mem_process_seqs] Processed 695734 reads in 131.841 CPU sec, 4.199 real sec
[M::mem_process_seqs] Processed 695180 reads in 83.006 CPU sec, 2.633 real sec
sorting block 9, #reads = 1091942 mem = 536877685 peak = 900374528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290008, 6, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (138, 200, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543)
[M::mem_pestat] mean and std.dev: (181.22, 65.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 678)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (163, 235, 336)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682)
[M::mem_pestat] mean and std.dev: (258.04, 121.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 855)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (328, 1584, 1959)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5221)
[M::mem_pestat] mean and std.dev: (1441.60, 995.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6852)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 701758 sequences (100000228 bp) in 2.108 CPU sec, 2.248 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291574, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.85, 115.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702110 sequences (100000017 bp) in 1.360 CPU sec, 1.365 real sec...
[M::mem_process_seqs] Processed 695030 reads in 133.910 CPU sec, 4.269 real sec
sorting block 10, #reads = 1091244 mem = 536877242 peak = 900374528
[M::mem_process_seqs] Processed 701486 reads in 84.492 CPU sec, 2.681 real sec
sorting block 11, #reads = 1092454 mem = 536879249 peak = 912773120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291656, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (241.87, 115.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 246, 885)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2249)
[M::mem_pestat] mean and std.dev: (389.92, 303.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2931)
[M::mem_pestat] skip orientation RR
[M::process] read 701174 sequences (100000047 bp) in 2.248 CPU sec, 2.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291836, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.26, 116.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700734 sequences (100000179 bp) in 1.400 CPU sec, 1.402 real sec...
[M::mem_process_seqs] Processed 701758 reads in 132.257 CPU sec, 4.211 real sec
[M::mem_process_seqs] Processed 702110 reads in 83.219 CPU sec, 2.642 real sec
sorting block 12, #reads = 1095706 mem = 536878600 peak = 912773120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292099, 10, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.28, 117.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (13, 78, 78)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 208)
[M::mem_pestat] mean and std.dev: (42.56, 32.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 273)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 401, 1199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3119)
[M::mem_pestat] mean and std.dev: (677.18, 625.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4079)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 700312 sequences (100000051 bp) in 2.285 CPU sec, 2.436 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291789, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (245.81, 118.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 200, 1064)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2810)
[M::mem_pestat] mean and std.dev: (468.90, 443.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3683)
[M::mem_pestat] skip orientation RR
[M::process] read 699056 sequences (100000050 bp) in 1.360 CPU sec, 1.356 real sec...
[M::mem_process_seqs] Processed 701174 reads in 131.951 CPU sec, 4.201 real sec
[M::mem_process_seqs] Processed 700734 reads in 82.930 CPU sec, 2.632 real sec
sorting block 13, #reads = 1097412 mem = 536877242 peak = 912773120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291518, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (248.44, 121.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698208 sequences (100000092 bp) in 2.364 CPU sec, 2.534 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291047, 13, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (251.74, 122.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 845)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (77, 178, 1097)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3137)
[M::mem_pestat] mean and std.dev: (447.92, 528.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4157)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 700312 reads in 132.090 CPU sec, 4.208 real sec
[M::process] read 698078 sequences (100000146 bp) in 1.452 CPU sec, 1.463 real sec...
[M::mem_process_seqs] Processed 699056 reads in 83.297 CPU sec, 2.635 real sec
sorting block 14, #reads = 1096671 mem = 536881782 peak = 912773120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290899, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 228, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677)
[M::mem_pestat] mean and std.dev: (253.73, 123.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 850)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 15, #reads = 1095225 mem = 536879269 peak = 912773120
[M::process] read 697460 sequences (100000157 bp) in 2.100 CPU sec, 2.294 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291003, 10, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 229, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678)
[M::mem_pestat] mean and std.dev: (254.29, 122.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 851)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 653, 1471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4165)
[M::mem_pestat] mean and std.dev: (866.40, 866.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5512)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 698208 reads in 132.388 CPU sec, 4.229 real sec
[M::process] read 696070 sequences (100000226 bp) in 1.462 CPU sec, 1.466 real sec...
[M::mem_process_seqs] Processed 698078 reads in 83.687 CPU sec, 2.650 real sec
sorting block 16, #reads = 1094764 mem = 536877752 peak = 912773120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291130, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (254.64, 122.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696264 sequences (100000179 bp) in 2.279 CPU sec, 2.393 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290359, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (256.83, 122.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695742 sequences (100000285 bp) in 1.365 CPU sec, 1.375 real sec...
[M::mem_process_seqs] Processed 697460 reads in 131.549 CPU sec, 4.192 real sec
[M::mem_process_seqs] Processed 696070 reads in 82.732 CPU sec, 2.622 real sec
sorting block 17, #reads = 1094430 mem = 536879375 peak = 912773120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290220, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (256.89, 122.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695602 sequences (100000017 bp) in 2.165 CPU sec, 2.307 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 289895, 7, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (190, 379, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1099)
[M::mem_pestat] mean and std.dev: (321.08, 159.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1402)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 234, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (257.14, 122.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 695886 sequences (100000057 bp) in 1.350 CPU sec, 1.352 real sec...
[M::mem_process_seqs] Processed 696264 reads in 133.413 CPU sec, 4.257 real sec
[M::mem_process_seqs] Processed 695742 reads in 84.259 CPU sec, 2.670 real sec
sorting block 18, #reads = 1092753 mem = 536881604 peak = 912773120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 289969, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.40, 120.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695870 sequences (100000138 bp) in 2.137 CPU sec, 2.284 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 290200, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (166, 230, 1180)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3208)
[M::mem_pestat] mean and std.dev: (458.53, 460.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4222)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (254.86, 120.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 695564 sequences (100000177 bp) in 1.370 CPU sec, 1.369 real sec...
[M::mem_process_seqs] Processed 695602 reads in 133.899 CPU sec, 4.274 real sec
sorting block 19, #reads = 1091194 mem = 536882064 peak = 912773120
[M::mem_process_seqs] Processed 695886 reads in 84.323 CPU sec, 2.675 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290147, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (254.15, 119.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 20, #reads = 1091801 mem = 536879172 peak = 996388864
[M::process] read 695712 sequences (100000265 bp) in 2.139 CPU sec, 2.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 289934, 4, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (191, 612, 612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1454)
[M::mem_pestat] mean and std.dev: (491.17, 322.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1875)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (255.16, 120.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (638, 965, 965)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1619)
[M::mem_pestat] mean and std.dev: (773.82, 223.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1946)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 700790 sequences (100000179 bp) in 1.390 CPU sec, 1.387 real sec...
[M::mem_process_seqs] Processed 695870 reads in 133.209 CPU sec, 4.250 real sec
[M::mem_process_seqs] Processed 695564 reads in 83.837 CPU sec, 2.660 real sec
sorting block 21, #reads = 1091870 mem = 536878852 peak = 996388864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290099, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (255.35, 121.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702278 sequences (100000227 bp) in 2.368 CPU sec, 2.521 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291717, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (244.63, 117.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 695712 reads in 134.167 CPU sec, 4.271 real sec
[M::process] read 701298 sequences (100000145 bp) in 1.370 CPU sec, 1.371 real sec...
[M::mem_process_seqs] Processed 700790 reads in 84.928 CPU sec, 2.690 real sec
sorting block 22, #reads = 1091579 mem = 536878312 peak = 996388864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292284, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.34, 114.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701238 sequences (100000109 bp) in 2.186 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291426, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (242.85, 115.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 802)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700478 sequences (100000274 bp) in 1.370 CPU sec, 1.365 real sec...
[M::mem_process_seqs] Processed 702278 reads in 134.001 CPU sec, 4.267 real sec
sorting block 23, #reads = 1094743 mem = 536878548 peak = 996388864
[M::mem_process_seqs] Processed 701298 reads in 84.784 CPU sec, 2.688 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291954, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (169, 259, 358)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736)
[M::mem_pestat] mean and std.dev: (252.54, 87.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.80, 116.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 24, #reads = 1094615 mem = 536879938 peak = 996388864
[M::process] read 699770 sequences (100000094 bp) in 2.192 CPU sec, 2.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291991, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.64, 118.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701238 reads in 134.444 CPU sec, 4.289 real sec
[M::process] read 698992 sequences (100000124 bp) in 1.490 CPU sec, 1.506 real sec...
[M::mem_process_seqs] Processed 700478 reads in 84.761 CPU sec, 2.677 real sec
sorting block 25, #reads = 1095126 mem = 536879142 peak = 996388864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291562, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (266, 371, 2747)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7709)
[M::mem_pestat] mean and std.dev: (1450.20, 2117.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 10190)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.46, 119.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698596 sequences (100000294 bp) in 2.139 CPU sec, 2.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291352, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.37, 122.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698222 sequences (100000052 bp) in 1.350 CPU sec, 1.351 real sec...
[M::mem_process_seqs] Processed 699770 reads in 132.782 CPU sec, 4.229 real sec
[M::mem_process_seqs] Processed 698992 reads in 83.878 CPU sec, 2.654 real sec
sorting block 26, #reads = 1094591 mem = 536881924 peak = 996388864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291398, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (252.50, 122.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697890 sequences (100000042 bp) in 2.211 CPU sec, 2.320 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291134, 9, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.32, 122.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (239, 622, 1846)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5060)
[M::mem_pestat] mean and std.dev: (967.58, 957.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6667)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698596 reads in 129.922 CPU sec, 4.137 real sec
[M::process] read 697766 sequences (100000173 bp) in 1.419 CPU sec, 1.432 real sec...
[M::mem_process_seqs] Processed 698222 reads in 82.626 CPU sec, 2.612 real sec
sorting block 27, #reads = 1094465 mem = 536879295 peak = 996388864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290746, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672)
[M::mem_pestat] mean and std.dev: (253.04, 121.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 843)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 242, 592)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1430)
[M::mem_pestat] mean and std.dev: (259.50, 136.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1849)
[M::mem_pestat] skip orientation RR
[M::process] read 697544 sequences (100000240 bp) in 2.386 CPU sec, 2.551 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290958, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.41, 121.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 335, 3219)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9339)
[M::mem_pestat] mean and std.dev: (2043.92, 3129.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 14563)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697890 reads in 129.306 CPU sec, 4.116 real sec
sorting block 28, #reads = 1094246 mem = 536880976 peak = 996388864
[M::process] read 697252 sequences (100000184 bp) in 1.491 CPU sec, 1.509 real sec...
[M::mem_process_seqs] Processed 697766 reads in 82.706 CPU sec, 2.612 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290561, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.53, 121.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 29, #reads = 1093991 mem = 536890040 peak = 996388864
[M::process] read 697266 sequences (100000088 bp) in 2.109 CPU sec, 2.246 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290492, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.07, 119.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697544 reads in 129.679 CPU sec, 4.130 real sec
[M::process] read 696854 sequences (100000182 bp) in 1.449 CPU sec, 1.453 real sec...
[M::mem_process_seqs] Processed 697252 reads in 82.663 CPU sec, 2.610 real sec
sorting block 30, #reads = 1092699 mem = 536879938 peak = 996388864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290240, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.25, 119.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 696450 sequences (100000222 bp) in 2.156 CPU sec, 2.277 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290140, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.45, 119.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 195, 1111)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3015)
[M::mem_pestat] mean and std.dev: (472.73, 515.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3967)
[M::mem_pestat] skip orientation RR
[M::process] read 695752 sequences (100000139 bp) in 1.350 CPU sec, 1.344 real sec...
[M::mem_process_seqs] Processed 697266 reads in 130.555 CPU sec, 4.150 real sec
[M::mem_process_seqs] Processed 696854 reads in 82.941 CPU sec, 2.621 real sec
sorting block 31, #reads = 1092469 mem = 536878945 peak = 996388864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290679, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 232, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (224.08, 82.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 231, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.89, 120.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 313, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1202)
[M::mem_pestat] mean and std.dev: (332.60, 164.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1541)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 695572 sequences (100000092 bp) in 2.050 CPU sec, 2.189 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290106, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (242, 323, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (80, 485)
[M::mem_pestat] mean and std.dev: (273.80, 72.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 566)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (255.05, 120.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (283, 444, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (339.44, 131.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 696450 reads in 128.448 CPU sec, 4.098 real sec
[M::mem_process_seqs] Processed 695752 reads in 81.705 CPU sec, 2.578 real sec
[M::process] read 700530 sequences (100000179 bp) in 1.518 CPU sec, 1.539 real sec...
sorting block 32, #reads = 1092507 mem = 536876091 peak = 996388864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 289304, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (254.91, 120.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 33, #reads = 1091994 mem = 536876374 peak = 996388864
[M::process] read 701858 sequences (100000081 bp) in 2.062 CPU sec, 2.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291265, 11, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (244.60, 116.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (539, 1703, 4171)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11435)
[M::mem_pestat] mean and std.dev: (2503.36, 2648.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 15067)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 695572 reads in 131.649 CPU sec, 4.195 real sec
[M::process] read 701244 sequences (100000022 bp) in 1.472 CPU sec, 1.493 real sec...
[M::mem_process_seqs] Processed 700530 reads in 84.616 CPU sec, 2.672 real sec
sorting block 34, #reads = 1090884 mem = 536880774 peak = 996388864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291805, 5, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.96, 116.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 434, 1274)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3328)
[M::mem_pestat] mean and std.dev: (658.54, 479.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4355)
[M::mem_pestat] skip orientation RR
[M::process] read 700818 sequences (100000036 bp) in 2.340 CPU sec, 2.449 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292087, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.23, 117.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701858 reads in 128.819 CPU sec, 4.099 real sec
[M::process] read 699882 sequences (100000231 bp) in 1.476 CPU sec, 1.507 real sec...
[M::mem_process_seqs] Processed 701244 reads in 81.928 CPU sec, 2.581 real sec
sorting block 35, #reads = 1096080 mem = 536877714 peak = 996388864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291952, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.34, 117.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699256 sequences (100000189 bp) in 2.082 CPU sec, 2.195 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291311, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.51, 119.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700818 reads in 128.594 CPU sec, 4.098 real sec
[M::process] read 698866 sequences (100000130 bp) in 1.454 CPU sec, 1.464 real sec...
[M::mem_process_seqs] Processed 699882 reads in 82.369 CPU sec, 2.597 real sec
sorting block 36, #reads = 1096366 mem = 536884979 peak = 996388864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 291293, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (249.91, 120.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 408, 1152)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3140)
[M::mem_pestat] mean and std.dev: (576.15, 436.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4134)
[M::mem_pestat] skip orientation RR
[M::process] read 698838 sequences (100000016 bp) in 2.150 CPU sec, 2.280 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290657, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.24, 121.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699256 reads in 128.339 CPU sec, 4.092 real sec
[M::mem_process_seqs] Processed 698866 reads in 82.037 CPU sec, 2.588 real sec
sorting block 37, #reads = 1095181 mem = 536878796 peak = 996388864
[M::process] read 698432 sequences (100000269 bp) in 1.554 CPU sec, 1.593 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291104, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.10, 121.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 38, #reads = 1094267 mem = 536882204 peak = 1008328704
[M::process] read 698116 sequences (100000001 bp) in 2.047 CPU sec, 2.157 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290461, 5, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.92, 122.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (123, 191, 1342)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3780)
[M::mem_pestat] mean and std.dev: (433.00, 557.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4999)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698838 reads in 129.003 CPU sec, 4.112 real sec
[M::mem_process_seqs] Processed 698432 reads in 82.470 CPU sec, 2.601 real sec
[M::process] read 698170 sequences (100000183 bp) in 1.564 CPU sec, 1.590 real sec...
sorting block 39, #reads = 1094093 mem = 536878014 peak = 1008328704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290797, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (251.86, 121.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 838)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698614 sequences (100000278 bp) in 2.110 CPU sec, 2.282 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291065, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.97, 120.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698116 reads in 129.543 CPU sec, 4.128 real sec
[M::process] read 698720 sequences (100000287 bp) in 1.495 CPU sec, 1.522 real sec...
[M::mem_process_seqs] Processed 698170 reads in 82.978 CPU sec, 2.618 real sec
sorting block 40, #reads = 1094202 mem = 536878712 peak = 1008328704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291122, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.59, 119.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 277, 383)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (241.55, 99.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1022)
[M::mem_pestat] skip orientation RR
[M::process] read 698196 sequences (100000135 bp) in 2.087 CPU sec, 2.241 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290676, 7, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.09, 118.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698614 reads in 129.218 CPU sec, 4.120 real sec
[M::process] read 697742 sequences (100000129 bp) in 1.480 CPU sec, 1.493 real sec...
[M::mem_process_seqs] Processed 698720 reads in 82.451 CPU sec, 2.605 real sec
sorting block 41, #reads = 1093677 mem = 536878321 peak = 1008328704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291008, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (248.06, 118.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 42, #reads = 1093861 mem = 536879581 peak = 1008328704
[M::process] read 697058 sequences (100000058 bp) in 2.140 CPU sec, 2.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290276, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (249.76, 118.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 1491, 1842)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5032)
[M::mem_pestat] mean and std.dev: (1016.80, 764.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6627)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698196 reads in 130.566 CPU sec, 4.157 real sec
[M::process] read 696982 sequences (100000106 bp) in 1.497 CPU sec, 1.507 real sec...
[M::mem_process_seqs] Processed 697742 reads in 83.048 CPU sec, 2.620 real sec
sorting block 43, #reads = 1093460 mem = 536878705 peak = 1008328704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290753, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 228, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (251.35, 119.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (496, 496, 719)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (50, 1165)
[M::mem_pestat] mean and std.dev: (487.00, 278.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1602)
[M::mem_pestat] skip orientation RR
[M::process] read 696524 sequences (100000169 bp) in 2.065 CPU sec, 2.175 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290418, 3, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (290, 297, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (402.18, 205.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.18, 120.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 697058 reads in 129.256 CPU sec, 4.113 real sec
[M::process] read 700820 sequences (100000117 bp) in 1.452 CPU sec, 1.465 real sec...
[M::mem_process_seqs] Processed 696982 reads in 82.373 CPU sec, 2.600 real sec
sorting block 44, #reads = 1091699 mem = 536879726 peak = 1008328704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290244, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (252.89, 120.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 706632 sequences (100000158 bp) in 2.361 CPU sec, 2.510 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291937, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (242.06, 115.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696524 reads in 133.205 CPU sec, 4.231 real sec
[M::process] read 705238 sequences (100000169 bp) in 1.407 CPU sec, 1.406 real sec...
[M::mem_process_seqs] Processed 700820 reads in 86.976 CPU sec, 2.749 real sec
sorting block 45, #reads = 1092843 mem = 536878160 peak = 1008328704
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293776, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (228.44, 108.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704090 sequences (100000137 bp) in 2.317 CPU sec, 2.412 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 293469, 10, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (207, 247, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600)
[M::mem_pestat] mean and std.dev: (256.67, 87.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 731)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599)
[M::mem_pestat] mean and std.dev: (231.98, 111.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 749)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (76, 573, 794)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2230)
[M::mem_pestat] mean and std.dev: (469.67, 509.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2948)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 1153, 1153)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3147)
[M::mem_pestat] mean and std.dev: (864.00, 654.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4144)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 704716 sequences (100000065 bp) in 1.370 CPU sec, 1.367 real sec...
[M::mem_process_seqs] Processed 706632 reads in 135.808 CPU sec, 4.314 real sec
sorting block 46, #reads = 1092531 mem = 536878068 peak = 1008328704
[M::mem_process_seqs] Processed 705238 reads in 85.031 CPU sec, 2.695 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292812, 8, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612)
[M::mem_pestat] mean and std.dev: (235.35, 113.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 766)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 47, #reads = 1096692 mem = 536877173 peak = 1077006336
[M::process] read 703394 sequences (100000180 bp) in 2.107 CPU sec, 2.247 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292890, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 208, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602)
[M::mem_pestat] mean and std.dev: (232.42, 111.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 753)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702726 sequences (100000091 bp) in 1.400 CPU sec, 1.399 real sec...
[M::mem_process_seqs] Processed 704090 reads in 134.301 CPU sec, 4.270 real sec
[M::mem_process_seqs] Processed 704716 reads in 84.517 CPU sec, 2.672 real sec
sorting block 48, #reads = 1095917 mem = 536877461 peak = 1077006336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292763, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622)
[M::mem_pestat] mean and std.dev: (237.98, 115.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 779)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700880 sequences (100000227 bp) in 2.100 CPU sec, 2.224 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292366, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (289, 652, 912)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2158)
[M::mem_pestat] mean and std.dev: (585.46, 314.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2781)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (240.15, 116.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702240 sequences (100000156 bp) in 1.380 CPU sec, 1.375 real sec...
[M::mem_process_seqs] Processed 703394 reads in 132.863 CPU sec, 4.226 real sec
[M::mem_process_seqs] Processed 702726 reads in 83.958 CPU sec, 2.654 real sec
sorting block 49, #reads = 1096799 mem = 536880732 peak = 1077006336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291747, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 217, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (244.29, 119.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701064 sequences (100000172 bp) in 2.121 CPU sec, 2.226 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292555, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629)
[M::mem_pestat] mean and std.dev: (240.31, 116.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 788)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700880 reads in 134.848 CPU sec, 4.292 real sec
[M::process] read 701162 sequences (100000189 bp) in 1.380 CPU sec, 1.383 real sec...
[M::mem_process_seqs] Processed 702240 reads in 84.746 CPU sec, 2.683 real sec
sorting block 50, #reads = 1094518 mem = 536883248 peak = 1077006336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291892, 6, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (141, 420, 992)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2694)
[M::mem_pestat] mean and std.dev: (633.10, 669.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3545)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (242.68, 117.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 51, #reads = 1095093 mem = 536880387 peak = 1077006336
[M::process] read 699732 sequences (100000093 bp) in 2.143 CPU sec, 2.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292208, 9, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.76, 119.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701064 reads in 135.597 CPU sec, 4.316 real sec
[M::process] read 698008 sequences (100000028 bp) in 1.439 CPU sec, 1.440 real sec...
[M::mem_process_seqs] Processed 701162 reads in 85.767 CPU sec, 2.709 real sec
sorting block 52, #reads = 1093478 mem = 536881644 peak = 1077006336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291551, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (249.07, 121.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 304, 4683)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13577)
[M::mem_pestat] mean and std.dev: (1578.64, 1928.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18024)
[M::mem_pestat] skip orientation RR
[M::process] read 697048 sequences (100000001 bp) in 2.188 CPU sec, 2.330 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290771, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 227, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674)
[M::mem_pestat] mean and std.dev: (253.01, 122.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699732 reads in 136.518 CPU sec, 4.343 real sec
[M::process] read 696668 sequences (100000055 bp) in 1.420 CPU sec, 1.426 real sec...
[M::mem_process_seqs] Processed 698008 reads in 86.707 CPU sec, 2.743 real sec
sorting block 53, #reads = 1092389 mem = 536880397 peak = 1077006336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290955, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 211, 339)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (239.00, 107.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.24, 122.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 696648 sequences (100000173 bp) in 2.139 CPU sec, 2.263 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290464, 11, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (210, 437, 1062)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2766)
[M::mem_pestat] mean and std.dev: (562.45, 430.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3618)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (255.05, 122.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (182, 555, 756)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1904)
[M::mem_pestat] mean and std.dev: (500.90, 472.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2478)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RF
[M::process] read 696738 sequences (100000141 bp) in 1.370 CPU sec, 1.377 real sec...
[M::mem_process_seqs] Processed 697048 reads in 137.317 CPU sec, 4.368 real sec
sorting block 54, #reads = 1090400 mem = 536879260 peak = 1077006336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290502, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673)
[M::mem_pestat] mean and std.dev: (254.55, 122.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 844)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 708298 sequences (100000201 bp) in 1.736 CPU sec, 1.744 real sec...
[M::mem_process_seqs] Processed 696648 reads in 80.427 CPU sec, 2.778 real sec
[M::mem_process_seqs] Processed 696668 reads in 191.385 CPU sec, 6.297 real sec
sorting block 55, #reads = 1090441 mem = 536878255 peak = 1077006336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290525, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 230, 332)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676)
[M::mem_pestat] mean and std.dev: (255.36, 123.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 848)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 708122 sequences (100000174 bp) in 2.139 CPU sec, 2.238 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 294215, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 203, 289)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575)
[M::mem_pestat] mean and std.dev: (224.84, 105.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 718)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 696738 reads in 155.601 CPU sec, 4.939 real sec
[M::process] read 705956 sequences (100000231 bp) in 1.482 CPU sec, 1.490 real sec...
[M::mem_process_seqs] Processed 708298 reads in 93.373 CPU sec, 2.946 real sec
sorting block 56, #reads = 1089752 mem = 536876258 peak = 1077006336
[M::process] read 705016 sequences (100000143 bp) in 2.360 CPU sec, 2.565 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293910, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 207, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599)
[M::mem_pestat] mean and std.dev: (231.51, 110.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 749)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703156 sequences (100000129 bp) in 1.400 CPU sec, 1.400 real sec...
[M::mem_process_seqs] Processed 705956 reads in 80.059 CPU sec, 2.521 real sec
sorting block 57, #reads = 1092143 mem = 536879293 peak = 1077006336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 294144, 7, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 203, 290)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578)
[M::mem_pestat] mean and std.dev: (225.63, 106.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 722)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293102, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608)
[M::mem_pestat] mean and std.dev: (233.63, 111.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 761)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702792 sequences (100000016 bp) in 1.742 CPU sec, 1.748 real sec...
[M::mem_process_seqs] Processed 708122 reads in 229.183 CPU sec, 7.275 real sec
sorting block 58, #reads = 1092416 mem = 536877167 peak = 1077006336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292706, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623)
[M::mem_pestat] mean and std.dev: (238.82, 115.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 780)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702226 sequences (100000273 bp) in 1.911 CPU sec, 1.914 real sec...
[M::mem_process_seqs] Processed 703156 reads in 78.017 CPU sec, 2.463 real sec
[M::mem_process_seqs] Processed 705016 reads in 218.885 CPU sec, 6.968 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292816, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (239.29, 115.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 59, #reads = 1092827 mem = 536880635 peak = 1077006336
[M::process] read 701718 sequences (100000056 bp) in 2.182 CPU sec, 2.336 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292551, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.44, 117.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702792 reads in 156.689 CPU sec, 4.982 real sec
[M::mem_process_seqs] Processed 702226 reads in 95.866 CPU sec, 3.026 real sec
[M::process] read 700554 sequences (100000156 bp) in 1.671 CPU sec, 1.716 real sec...
sorting block 60, #reads = 1091629 mem = 536886955 peak = 1077006336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 292529, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (171, 265, 390)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 828)
[M::mem_pestat] mean and std.dev: (243.91, 108.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1047)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.17, 117.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700644 sequences (100000084 bp) in 2.257 CPU sec, 2.394 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291738, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (244.77, 118.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700082 sequences (100000196 bp) in 1.390 CPU sec, 1.386 real sec...
[M::mem_process_seqs] Processed 701718 reads in 139.969 CPU sec, 4.447 real sec
[M::mem_process_seqs] Processed 700554 reads in 87.746 CPU sec, 2.774 real sec
sorting block 61, #reads = 1090537 mem = 536878264 peak = 1077006336
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291759, 8, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.70, 119.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (169, 197, 1942)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5488)
[M::mem_pestat] mean and std.dev: (627.69, 731.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7261)
[M::mem_pestat] skip orientation RR
[M::process] read 698700 sequences (100000007 bp) in 2.221 CPU sec, 2.328 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291580, 8, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (192, 3904, 4623)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13485)
[M::mem_pestat] mean and std.dev: (2440.60, 2070.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 17916)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (247.48, 120.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697672 sequences (100000089 bp) in 1.380 CPU sec, 1.382 real sec...
[M::mem_process_seqs] Processed 700644 reads in 139.377 CPU sec, 4.434 real sec
sorting block 62, #reads = 1092562 mem = 536881858 peak = 1088712704
[M::mem_process_seqs] Processed 700082 reads in 87.170 CPU sec, 2.758 real sec
sorting block 63, #reads = 1091829 mem = 536879582 peak = 1133420544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290860, 9, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671)
[M::mem_pestat] mean and std.dev: (252.41, 123.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 842)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 225, 277)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (46, 431)
[M::mem_pestat] mean and std.dev: (205.10, 48.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 508)
[M::mem_pestat] skip orientation RR
[M::process] read 697738 sequences (100000034 bp) in 2.185 CPU sec, 2.304 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290929, 11, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 331)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (254.01, 123.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 847)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (160, 787, 1387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3841)
[M::mem_pestat] mean and std.dev: (846.73, 721.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5068)
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (190, 271, 663)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1609)
[M::mem_pestat] mean and std.dev: (527.42, 516.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2594)
[M::mem_pestat] skip orientation RF
[M::mem_pestat] skip orientation RR
[M::process] read 698282 sequences (100000224 bp) in 1.410 CPU sec, 1.406 real sec...
[M::mem_process_seqs] Processed 698700 reads in 136.081 CPU sec, 4.328 real sec
[M::mem_process_seqs] Processed 697672 reads in 86.573 CPU sec, 2.739 real sec
sorting block 64, #reads = 1092009 mem = 536879065 peak = 1133420544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 290571, 9, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 211, 846)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2212)
[M::mem_pestat] mean and std.dev: (304.44, 251.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2895)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (251.79, 122.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698882 sequences (100000010 bp) in 2.387 CPU sec, 2.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290940, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (249.41, 120.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 707004 sequences (100000207 bp) in 1.420 CPU sec, 1.414 real sec...
[M::mem_process_seqs] Processed 697738 reads in 141.048 CPU sec, 4.477 real sec
[M::mem_process_seqs] Processed 698282 reads in 90.690 CPU sec, 2.869 real sec
sorting block 65, #reads = 1090966 mem = 536882330 peak = 1133420544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290593, 4, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.64, 120.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 292, 673)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1581)
[M::mem_pestat] mean and std.dev: (363.50, 188.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2035)
[M::mem_pestat] skip orientation RR
[M::process] read 705476 sequences (100000103 bp) in 2.272 CPU sec, 2.388 real sec...
[M::process] read 703830 sequences (100000202 bp) in 1.410 CPU sec, 1.411 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 293749, 4, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (224, 248, 1482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3998)
[M::mem_pestat] mean and std.dev: (461.50, 513.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5256)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 205, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (228.24, 108.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698882 reads in 155.945 CPU sec, 4.963 real sec
sorting block 66, #reads = 1090059 mem = 536879469 peak = 1133420544
[M::mem_process_seqs] Processed 707004 reads in 92.815 CPU sec, 2.941 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293316, 7, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 208, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601)
[M::mem_pestat] mean and std.dev: (231.74, 110.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 752)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 67, #reads = 1088803 mem = 536880957 peak = 1133420544
[M::process] read 703190 sequences (100000019 bp) in 2.311 CPU sec, 2.395 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292740, 3, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 212, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616)
[M::mem_pestat] mean and std.dev: (236.56, 113.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 771)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 834, 2949)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8435)
[M::mem_pestat] mean and std.dev: (1155.42, 1234.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 11178)
[M::mem_pestat] skip orientation RR
[M::process] read 702388 sequences (100000028 bp) in 1.400 CPU sec, 1.406 real sec...
[M::mem_process_seqs] Processed 705476 reads in 143.718 CPU sec, 4.572 real sec
[M::mem_process_seqs] Processed 703830 reads in 88.786 CPU sec, 2.815 real sec
sorting block 68, #reads = 1096833 mem = 536879494 peak = 1133420544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292200, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 309)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625)
[M::mem_pestat] mean and std.dev: (237.99, 114.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 783)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701770 sequences (100000079 bp) in 2.450 CPU sec, 2.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292429, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.58, 116.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701344 sequences (100000142 bp) in 1.400 CPU sec, 1.405 real sec...
[M::mem_process_seqs] Processed 703190 reads in 141.409 CPU sec, 4.493 real sec
[M::mem_process_seqs] Processed 702388 reads in 88.142 CPU sec, 2.790 real sec
sorting block 69, #reads = 1095771 mem = 536879466 peak = 1133420544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292285, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (243.01, 117.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 459, 1434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3912)
[M::mem_pestat] mean and std.dev: (850.00, 850.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5151)
[M::mem_pestat] skip orientation RR
[M::process] read 701080 sequences (100000062 bp) in 2.136 CPU sec, 2.234 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291730, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.55, 118.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700454 sequences (100000002 bp) in 1.400 CPU sec, 1.400 real sec...
[M::mem_process_seqs] Processed 701770 reads in 142.664 CPU sec, 4.543 real sec
sorting block 70, #reads = 1095965 mem = 536880941 peak = 1133420544
[M::mem_process_seqs] Processed 701344 reads in 90.281 CPU sec, 2.860 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291973, 7, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.83, 118.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 409, 2111)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5951)
[M::mem_pestat] mean and std.dev: (698.67, 771.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7871)
[M::mem_pestat] skip orientation RR
sorting block 71, #reads = 1093838 mem = 536879300 peak = 1133420544
[M::process] read 699640 sequences (100000072 bp) in 2.478 CPU sec, 2.593 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291748, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (246.98, 120.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 819)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699298 sequences (100000254 bp) in 1.420 CPU sec, 1.425 real sec...
[M::mem_process_seqs] Processed 701080 reads in 143.422 CPU sec, 4.557 real sec
[M::mem_process_seqs] Processed 700454 reads in 90.020 CPU sec, 2.852 real sec
sorting block 72, #reads = 1093765 mem = 536879287 peak = 1133420544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291470, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.71, 121.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698390 sequences (100000033 bp) in 2.390 CPU sec, 2.505 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291214, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.67, 121.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698530 sequences (100000221 bp) in 1.410 CPU sec, 1.406 real sec...
[M::mem_process_seqs] Processed 699640 reads in 143.833 CPU sec, 4.571 real sec
[M::mem_process_seqs] Processed 699298 reads in 90.252 CPU sec, 2.862 real sec
sorting block 73, #reads = 1092338 mem = 536877451 peak = 1133420544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290585, 7, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.26, 120.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 240, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (41, 476)
[M::mem_pestat] mean and std.dev: (222.00, 69.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 563)
[M::mem_pestat] skip orientation RR
[M::process] read 698618 sequences (100000162 bp) in 2.429 CPU sec, 2.512 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291102, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658)
[M::mem_pestat] mean and std.dev: (249.34, 119.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 825)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698580 sequences (100000102 bp) in 1.410 CPU sec, 1.413 real sec...
[M::mem_process_seqs] Processed 698390 reads in 146.199 CPU sec, 4.647 real sec
[M::mem_process_seqs] Processed 698530 reads in 91.109 CPU sec, 2.890 real sec
sorting block 74, #reads = 1091824 mem = 536879713 peak = 1133420544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291199, 2, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (257, 405, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809)
[M::mem_pestat] mean and std.dev: (335.20, 171.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.62, 119.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698298 sequences (100000223 bp) in 2.477 CPU sec, 2.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290407, 4, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.05, 119.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (192, 444, 764)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1908)
[M::mem_pestat] mean and std.dev: (403.92, 248.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2480)
[M::mem_pestat] skip orientation RR
[M::process] read 700996 sequences (100000022 bp) in 1.460 CPU sec, 1.462 real sec...
[M::mem_process_seqs] Processed 698618 reads in 147.968 CPU sec, 4.701 real sec
sorting block 75, #reads = 1090933 mem = 536877037 peak = 1133420544
[M::mem_process_seqs] Processed 698580 reads in 92.761 CPU sec, 2.942 real sec
sorting block 76, #reads = 1089844 mem = 536879237 peak = 1133420544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290987, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.98, 119.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (119, 222, 353)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821)
[M::mem_pestat] mean and std.dev: (264.20, 149.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1055)
[M::mem_pestat] skip orientation RR
[M::process] read 709952 sequences (100000231 bp) in 2.202 CPU sec, 2.262 real sec...
[M::process] read 709018 sequences (100000109 bp) in 1.480 CPU sec, 1.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291100, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (126, 195, 401)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (218.00, 114.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (242.22, 116.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698298 reads in 158.303 CPU sec, 5.042 real sec
[M::mem_process_seqs] Processed 700996 reads in 104.765 CPU sec, 3.327 real sec
sorting block 77, #reads = 1087919 mem = 536882407 peak = 1133420544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 294619, 2, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (183, 202, 623)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1503)
[M::mem_pestat] mean and std.dev: (358.40, 324.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1943)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 199, 284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564)
[M::mem_pestat] mean and std.dev: (221.24, 103.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 704)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 706214 sequences (100000110 bp) in 2.451 CPU sec, 2.532 real sec...
[M::process] read 704826 sequences (100000088 bp) in 1.450 CPU sec, 1.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 294336, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 288)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574)
[M::mem_pestat] mean and std.dev: (224.08, 105.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 717)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (139, 262, 360)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802)
[M::mem_pestat] mean and std.dev: (209.00, 81.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1023)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 709952 reads in 159.354 CPU sec, 5.066 real sec
[M::mem_process_seqs] Processed 709018 reads in 100.854 CPU sec, 3.208 real sec
sorting block 78, #reads = 1086925 mem = 536878173 peak = 1133420544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292981, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 299)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601)
[M::mem_pestat] mean and std.dev: (231.24, 110.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 752)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703090 sequences (100000023 bp) in 2.431 CPU sec, 2.520 real sec...
[M::process] read 702444 sequences (100000222 bp) in 1.420 CPU sec, 1.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293676, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609)
[M::mem_pestat] mean and std.dev: (234.51, 112.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 762)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 706214 reads in 149.554 CPU sec, 4.752 real sec
sorting block 79, #reads = 1089994 mem = 536881883 peak = 1133420544
[M::mem_process_seqs] Processed 704826 reads in 93.226 CPU sec, 2.959 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292394, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 213, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626)
[M::mem_pestat] mean and std.dev: (238.84, 114.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 784)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 80, #reads = 1092016 mem = 536877649 peak = 1133420544
[M::process] read 701120 sequences (100000069 bp) in 2.235 CPU sec, 2.326 real sec...
[M::process] read 700858 sequences (100000006 bp) in 1.450 CPU sec, 1.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292492, 3, 17)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 216, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (241.57, 116.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (176, 176, 403)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857)
[M::mem_pestat] mean and std.dev: (234.67, 92.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1084)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 703090 reads in 148.029 CPU sec, 4.710 real sec
[M::mem_process_seqs] Processed 702444 reads in 93.692 CPU sec, 2.975 real sec
sorting block 81, #reads = 1091564 mem = 536878701 peak = 1133420544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292106, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.27, 119.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700324 sequences (100000232 bp) in 2.248 CPU sec, 2.332 real sec...
[M::process] read 700198 sequences (100000287 bp) in 1.430 CPU sec, 1.422 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291916, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (245.40, 119.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701120 reads in 147.493 CPU sec, 4.691 real sec
[M::mem_process_seqs] Processed 700858 reads in 92.665 CPU sec, 2.945 real sec
sorting block 82, #reads = 1091465 mem = 536890665 peak = 1133420544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291463, 6, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (245.37, 118.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 247, 1834)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5062)
[M::mem_pestat] mean and std.dev: (869.00, 1095.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6676)
[M::mem_pestat] skip orientation RR
[M::process] read 700178 sequences (100000241 bp) in 2.466 CPU sec, 2.548 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291645, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (245.47, 118.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699276 sequences (100000133 bp) in 1.410 CPU sec, 1.412 real sec...
[M::mem_process_seqs] Processed 700324 reads in 146.329 CPU sec, 4.651 real sec
[M::mem_process_seqs] Processed 700198 reads in 91.131 CPU sec, 2.887 real sec
sorting block 83, #reads = 1090697 mem = 536878929 peak = 1133420544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291667, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641)
[M::mem_pestat] mean and std.dev: (244.64, 118.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 803)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698888 sequences (100000055 bp) in 2.477 CPU sec, 2.566 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 290938, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (225, 320, 337)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561)
[M::mem_pestat] mean and std.dev: (302.71, 113.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 756)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.85, 119.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698692 sequences (100000110 bp) in 1.420 CPU sec, 1.415 real sec...
[M::mem_process_seqs] Processed 700178 reads in 144.015 CPU sec, 4.577 real sec
sorting block 84, #reads = 1091159 mem = 536884176 peak = 1133420544
[M::mem_process_seqs] Processed 699276 reads in 89.343 CPU sec, 2.833 real sec
sorting block 85, #reads = 1091765 mem = 536877044 peak = 1133420544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290981, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (246.73, 117.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698064 sequences (100000223 bp) in 2.475 CPU sec, 2.534 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291063, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.16, 119.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 655632 sequences (93881834 bp) in 1.330 CPU sec, 1.330 real sec...
[M::mem_process_seqs] Processed 698888 reads in 141.640 CPU sec, 4.505 real sec
[M::mem_process_seqs] Processed 698692 reads in 88.316 CPU sec, 2.799 real sec
sorting block 86, #reads = 1091374 mem = 536881502 peak = 1133420544
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290660, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (249.42, 119.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 272963, 5, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (159, 313, 351)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735)
[M::mem_pestat] mean and std.dev: (274.44, 180.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 998)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.78, 120.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698064 reads in 134.357 CPU sec, 4.358 real sec
[M::mem_process_seqs] Processed 655632 reads in 83.238 CPU sec, 2.694 real sec
sorting block 87, #reads = 1091926 mem = 536879606 peak = 1133420544
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 96542928
overall: 70014353408 mem 11616.450 user 408.822 sys 997.672 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L003\tPU:L003\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 997.710 sec; CPU: 12025.275 sec
sorting block 88, #reads = 851610 mem = 419979525 peak = 1189056512
uniq header count 1
merging 89 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5183468897
output reads: 97036580
bam_mem_sort: 89 calls 31.384 user 0.219 sys 31.828 real
bam_write: 89 calls 47.016 user 1.557 sys 48.981 real
execute: 1 calls 18.914 user 1.167 sys 1025.032 real
merge_files: 1 calls 18.897 user 1.135 sys 26.471 real
parse_chunk: 1421 calls 43.843 user 12.831 sys 58.141 real
read_chunk: 3838 calls 35.730 user 34.221 sys 343.111 real
sort_block: 1 calls 28.161 user 33.620 sys 331.450 real
write_chunk: 2415 calls 18.489 user 0.853 sys 25.569 real
overall: 3024379904 mem 1031.048 user 57.894 sys 1025.063 real
ls: cannot access '*.cram': No such file or directory