cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 659_d0M-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 2090.018 CPU sec, 74.950 real sec
[M::process] read 678518 sequences (100000289 bp) in 0.997 CPU sec, 1.001 real sec...
[M::process] read 677746 sequences (100000012 bp) in 1.605 CPU sec, 1.618 real sec...
[M::process] read 677370 sequences (100000040 bp) in 1.575 CPU sec, 1.609 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285161, 2, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (239, 358, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833)
[M::mem_pestat] mean and std.dev: (316.62, 100.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1031)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 307, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (328.91, 151.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1068)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 280, 377)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847)
[M::mem_pestat] mean and std.dev: (259.11, 108.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1082)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 676886 sequences (100000033 bp) in 1.517 CPU sec, 1.525 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285182, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 315, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (335.18, 153.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284547, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 317, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (338.14, 155.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1101)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678518 reads in 271.604 CPU sec, 8.755 real sec
[M::mem_process_seqs] Processed 677746 reads in 183.109 CPU sec, 5.926 real sec
[M::mem_process_seqs] Processed 677370 reads in 200.195 CPU sec, 6.502 real sec
sorting block 0, #reads = 1082264 mem = 536880652 peak = 576647168
[M::process] read 676762 sequences (100000036 bp) in 1.967 CPU sec, 2.228 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284680, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 321, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 888)
[M::mem_pestat] mean and std.dev: (341.19, 156.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676642 sequences (100000129 bp) in 1.723 CPU sec, 1.787 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285222, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 324, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (343.99, 157.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1124)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676538 sequences (100000243 bp) in 1.439 CPU sec, 1.440 real sec...
[M::mem_process_seqs] Processed 676886 reads in 171.252 CPU sec, 5.464 real sec
sorting block 1, #reads = 1081291 mem = 536879360 peak = 846950400
[M::mem_process_seqs] Processed 676762 reads in 109.768 CPU sec, 3.473 real sec
[M::process] read 676190 sequences (100000134 bp) in 1.907 CPU sec, 2.069 real sec...
sorting block 2, #reads = 1082067 mem = 536880831 peak = 946724864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285040, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 325, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (345.67, 158.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676036 sequences (100000098 bp) in 1.743 CPU sec, 1.808 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284379, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (347.71, 159.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676642 reads in 171.506 CPU sec, 5.723 real sec
[M::mem_process_seqs] Processed 676538 reads in 109.621 CPU sec, 3.697 real sec
sorting block 3, #reads = 1082563 mem = 536877981 peak = 946724864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284480, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (349.09, 159.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675708 sequences (100000021 bp) in 2.281 CPU sec, 2.434 real sec...
[M::process] read 675876 sequences (100000107 bp) in 1.669 CPU sec, 1.678 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284719, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 332, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (350.49, 159.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676190 reads in 174.735 CPU sec, 5.794 real sec
[M::mem_process_seqs] Processed 676036 reads in 110.010 CPU sec, 3.648 real sec
sorting block 4, #reads = 1081766 mem = 536879526 peak = 946724864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284760, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (352.25, 160.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675600 sequences (100000157 bp) in 2.004 CPU sec, 2.228 real sec...
[M::process] read 675552 sequences (100000172 bp) in 1.660 CPU sec, 1.682 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284459, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (781, 781, 781)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (781, 781)
[M::mem_pestat] mean and std.dev: (781.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (781, 781)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (351.95, 160.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675708 reads in 175.008 CPU sec, 5.771 real sec
[M::mem_process_seqs] Processed 675876 reads in 112.880 CPU sec, 3.722 real sec
sorting block 5, #reads = 1081645 mem = 536879485 peak = 946724864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284626, 4, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (174, 400, 400)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (326.80, 112.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1078)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (351.83, 160.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675770 sequences (100000202 bp) in 2.332 CPU sec, 2.523 real sec...
[M::process] read 676084 sequences (100000133 bp) in 1.691 CPU sec, 1.705 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284313, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (352.30, 161.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675600 reads in 176.817 CPU sec, 5.894 real sec
sorting block 6, #reads = 1081912 mem = 536880943 peak = 946724864
[M::mem_process_seqs] Processed 675552 reads in 114.060 CPU sec, 3.814 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284331, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (349.82, 160.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 295, 898)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2184)
[M::mem_pestat] mean and std.dev: (457.21, 292.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2827)
[M::mem_pestat] skip orientation RR
sorting block 7, #reads = 1081685 mem = 536876457 peak = 946724864
[M::process] read 676042 sequences (100000043 bp) in 2.114 CPU sec, 2.337 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284455, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (348.27, 159.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676056 sequences (100000117 bp) in 1.815 CPU sec, 1.825 real sec...
[M::mem_process_seqs] Processed 675770 reads in 178.494 CPU sec, 6.057 real sec
[M::mem_process_seqs] Processed 676084 reads in 114.866 CPU sec, 3.834 real sec
sorting block 8, #reads = 1080751 mem = 536880797 peak = 946724864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284690, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 325, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (346.17, 158.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676062 sequences (100000252 bp) in 2.390 CPU sec, 2.655 real sec...
[M::process] read 676036 sequences (100000058 bp) in 1.858 CPU sec, 1.914 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284443, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 325, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (345.74, 157.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676042 reads in 179.784 CPU sec, 6.161 real sec
sorting block 9, #reads = 1081129 mem = 536878688 peak = 946724864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284127, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 327, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (347.88, 159.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675900 sequences (100000099 bp) in 1.821 CPU sec, 1.829 real sec...
[M::mem_process_seqs] Processed 676062 reads in 99.601 CPU sec, 3.389 real sec
[M::mem_process_seqs] Processed 676056 reads in 256.366 CPU sec, 8.728 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283984, 4, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (348.25, 158.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 457, 656)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1576)
[M::mem_pestat] mean and std.dev: (427.70, 219.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2036)
[M::mem_pestat] skip orientation RR
sorting block 10, #reads = 1081462 mem = 536884669 peak = 946724864
[M::process] read 675730 sequences (100000144 bp) in 2.465 CPU sec, 2.740 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284383, 9, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (348.56, 158.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676036 reads in 198.850 CPU sec, 6.772 real sec
[M::process] read 675654 sequences (100000129 bp) in 1.724 CPU sec, 1.763 real sec...
[M::mem_process_seqs] Processed 675900 reads in 117.547 CPU sec, 3.943 real sec
sorting block 11, #reads = 1081171 mem = 536877973 peak = 946724864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283878, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 327, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906)
[M::mem_pestat] mean and std.dev: (348.37, 158.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1133)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674812 sequences (100000142 bp) in 2.368 CPU sec, 2.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284108, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (349.68, 158.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675306 sequences (100000210 bp) in 1.696 CPU sec, 1.725 real sec...
[M::mem_process_seqs] Processed 675730 reads in 180.809 CPU sec, 6.147 real sec
sorting block 12, #reads = 1081113 mem = 536878432 peak = 946724864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283938, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (354.11, 158.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675094 sequences (100000243 bp) in 1.967 CPU sec, 1.979 real sec...
[M::mem_process_seqs] Processed 674812 reads in 103.620 CPU sec, 3.749 real sec
[M::mem_process_seqs] Processed 675654 reads in 258.238 CPU sec, 8.977 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283874, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 329, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (348.77, 157.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 13, #reads = 1081309 mem = 536882168 peak = 946724864
[M::process] read 675372 sequences (100000007 bp) in 2.362 CPU sec, 2.637 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284408, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 329, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899)
[M::mem_pestat] mean and std.dev: (349.03, 156.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1123)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675306 reads in 194.721 CPU sec, 6.516 real sec
[M::mem_process_seqs] Processed 675094 reads in 113.267 CPU sec, 3.766 real sec
[M::process] read 675238 sequences (100000002 bp) in 2.171 CPU sec, 2.273 real sec...
sorting block 14, #reads = 1082114 mem = 536880088 peak = 946724864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284272, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 327, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 884)
[M::mem_pestat] mean and std.dev: (346.40, 154.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1103)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675078 sequences (100000090 bp) in 2.062 CPU sec, 2.354 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284577, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 331, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897)
[M::mem_pestat] mean and std.dev: (350.03, 157.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1120)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (1143, 1143, 8284)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22566)
[M::mem_pestat] mean and std.dev: (3709.77, 3630.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 29707)
[M::mem_pestat] skip orientation RR
[M::process] read 674600 sequences (100000129 bp) in 1.807 CPU sec, 1.817 real sec...
[M::mem_process_seqs] Processed 675372 reads in 170.874 CPU sec, 5.582 real sec
[M::mem_process_seqs] Processed 675238 reads in 109.413 CPU sec, 3.536 real sec
sorting block 15, #reads = 1082199 mem = 536880925 peak = 946724864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284580, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 333, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (351.83, 158.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1129)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674616 sequences (100000041 bp) in 2.598 CPU sec, 2.905 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283934, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 336, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (355.01, 159.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675078 reads in 168.702 CPU sec, 5.552 real sec
[M::process] read 674912 sequences (100000131 bp) in 1.782 CPU sec, 1.787 real sec...
sorting block 16, #reads = 1081687 mem = 536879367 peak = 946724864
[M::mem_process_seqs] Processed 674600 reads in 108.417 CPU sec, 3.509 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284459, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 339, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (357.90, 161.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 17, #reads = 1081382 mem = 536877306 peak = 983814144
[M::process] read 677964 sequences (100000147 bp) in 2.333 CPU sec, 2.685 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284793, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (352.44, 158.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1130)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (451, 451, 576)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (201, 826)
[M::mem_pestat] mean and std.dev: (457.50, 80.66)
[M::mem_pestat] low and high boundaries for proper pairs: (76, 951)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674616 reads in 172.031 CPU sec, 5.609 real sec
[M::process] read 677482 sequences (100000032 bp) in 1.815 CPU sec, 1.837 real sec...
[M::mem_process_seqs] Processed 674912 reads in 114.038 CPU sec, 3.674 real sec
sorting block 18, #reads = 1081791 mem = 536877027 peak = 983814144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284971, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 313, 436)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (335.33, 155.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677164 sequences (100000253 bp) in 2.588 CPU sec, 3.013 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284638, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 318, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (340.01, 157.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677964 reads in 176.144 CPU sec, 5.766 real sec
[M::mem_process_seqs] Processed 677482 reads in 114.155 CPU sec, 3.702 real sec
[M::process] read 676800 sequences (100000149 bp) in 1.901 CPU sec, 1.954 real sec...
sorting block 19, #reads = 1080806 mem = 536877811 peak = 983814144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284724, 4, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 280, 1492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4104)
[M::mem_pestat] mean and std.dev: (664.40, 590.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5410)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 322, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (343.09, 158.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1127)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676664 sequences (100000202 bp) in 2.218 CPU sec, 2.631 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284970, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 325, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (345.92, 159.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677164 reads in 171.957 CPU sec, 5.606 real sec
[M::process] read 676534 sequences (100000067 bp) in 2.039 CPU sec, 2.082 real sec...
[M::mem_process_seqs] Processed 676800 reads in 111.360 CPU sec, 3.574 real sec
sorting block 20, #reads = 1083010 mem = 536885414 peak = 983814144
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284771, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (346.55, 160.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676190 sequences (100000005 bp) in 2.295 CPU sec, 2.717 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284560, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 326, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (347.24, 159.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676664 reads in 173.634 CPU sec, 5.630 real sec
sorting block 21, #reads = 1083319 mem = 536880441 peak = 983814144
[M::mem_process_seqs] Processed 676534 reads in 112.884 CPU sec, 3.608 real sec
[M::process] read 675872 sequences (100000068 bp) in 2.717 CPU sec, 2.911 real sec...
sorting block 22, #reads = 1082727 mem = 536878781 peak = 1000525824
[M::process] read 675864 sequences (100000238 bp) in 2.112 CPU sec, 2.759 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284657, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (351.98, 161.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1161)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675872 reads in 98.543 CPU sec, 3.316 real sec
[M::process] read 675982 sequences (100000082 bp) in 2.521 CPU sec, 2.815 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284555, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (351.00, 161.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284670, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (352.15, 160.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676190 reads in 267.141 CPU sec, 8.885 real sec
[M::mem_process_seqs] Processed 675864 reads in 128.581 CPU sec, 4.348 real sec
sorting block 23, #reads = 1082387 mem = 536882695 peak = 1000525824
[M::process] read 675648 sequences (100000291 bp) in 2.073 CPU sec, 2.579 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284631, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (352.99, 162.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 24, #reads = 1082947 mem = 536880155 peak = 1000525824
[M::process] read 675498 sequences (100000280 bp) in 2.896 CPU sec, 3.188 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283929, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (354.77, 162.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675982 reads in 176.087 CPU sec, 5.832 real sec
[M::mem_process_seqs] Processed 675648 reads in 113.775 CPU sec, 3.747 real sec
[M::process] read 675822 sequences (100000026 bp) in 1.874 CPU sec, 2.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284176, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (356.24, 163.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 25, #reads = 1082389 mem = 536877960 peak = 1000525824
[M::process] read 675736 sequences (100000172 bp) in 2.423 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284428, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 334, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (354.66, 163.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675498 reads in 178.878 CPU sec, 5.961 real sec
[M::mem_process_seqs] Processed 675822 reads in 115.739 CPU sec, 3.837 real sec
[M::process] read 675976 sequences (100000179 bp) in 2.189 CPU sec, 2.411 real sec...
sorting block 26, #reads = 1081418 mem = 536880850 peak = 1000525824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284275, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (351.48, 161.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675986 sequences (100000267 bp) in 2.375 CPU sec, 2.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284595, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 327, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (349.04, 160.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675736 reads in 177.293 CPU sec, 5.885 real sec
[M::process] read 676108 sequences (100000191 bp) in 2.078 CPU sec, 2.506 real sec...
sorting block 27, #reads = 1081741 mem = 536878935 peak = 1000525824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284787, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (350.69, 162.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675986 reads in 96.591 CPU sec, 3.154 real sec
[M::process] read 675948 sequences (100000109 bp) in 2.379 CPU sec, 2.649 real sec...
[M::mem_process_seqs] Processed 675976 reads in 273.472 CPU sec, 9.021 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284614, 1, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (478, 493, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (384, 619)
[M::mem_pestat] mean and std.dev: (501.00, 19.41)
[M::mem_pestat] low and high boundaries for proper pairs: (337, 666)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (349.20, 161.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 28, #reads = 1082676 mem = 536877698 peak = 1000525824
[M::process] read 675960 sequences (100000057 bp) in 2.558 CPU sec, 2.905 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284764, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (348.96, 160.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676108 reads in 195.687 CPU sec, 6.403 real sec
[M::process] read 675984 sequences (100000282 bp) in 2.318 CPU sec, 2.598 real sec...
sorting block 29, #reads = 1081479 mem = 536877131 peak = 1000525824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284747, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (349.44, 160.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675960 reads in 99.621 CPU sec, 3.233 real sec
[M::process] read 675818 sequences (100000046 bp) in 2.564 CPU sec, 2.751 real sec...
[M::mem_process_seqs] Processed 675948 reads in 285.225 CPU sec, 9.339 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284716, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (349.41, 160.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 30, #reads = 1081660 mem = 536879913 peak = 1000525824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284648, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (349.78, 160.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675850 sequences (100000292 bp) in 2.542 CPU sec, 2.920 real sec...
[M::mem_process_seqs] Processed 675984 reads in 192.965 CPU sec, 6.387 real sec
[M::mem_process_seqs] Processed 675818 reads in 115.939 CPU sec, 3.894 real sec
[M::process] read 675554 sequences (100000080 bp) in 2.258 CPU sec, 2.803 real sec...
sorting block 31, #reads = 1082408 mem = 536882416 peak = 1000525824
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 284181, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (348.28, 159.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284107, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (352.05, 161.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675786 sequences (100000224 bp) in 2.567 CPU sec, 3.078 real sec...
[M::mem_process_seqs] Processed 675850 reads in 178.668 CPU sec, 5.923 real sec
[M::mem_process_seqs] Processed 675554 reads in 116.219 CPU sec, 3.829 real sec
sorting block 32, #reads = 1081872 mem = 536878990 peak = 1000525824
[M::process] read 675502 sequences (100000265 bp) in 2.416 CPU sec, 2.863 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285085, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918)
[M::mem_pestat] mean and std.dev: (351.51, 160.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675428 sequences (100000045 bp) in 2.300 CPU sec, 2.561 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284451, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (352.30, 161.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (114, 127, 243)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501)
[M::mem_pestat] mean and std.dev: (138.00, 42.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 630)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675502 reads in 99.361 CPU sec, 3.450 real sec
sorting block 33, #reads = 1080687 mem = 536879519 peak = 1000525824
[M::process] read 675254 sequences (100000025 bp) in 1.988 CPU sec, 2.189 real sec...
[M::mem_process_seqs] Processed 675786 reads in 269.988 CPU sec, 9.365 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284846, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (351.76, 160.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 34, #reads = 1081960 mem = 536880015 peak = 1002225664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284561, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (351.51, 160.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675242 sequences (100000142 bp) in 2.669 CPU sec, 2.931 real sec...
[M::mem_process_seqs] Processed 675428 reads in 178.950 CPU sec, 5.947 real sec
[M::mem_process_seqs] Processed 675254 reads in 115.793 CPU sec, 3.802 real sec
[M::process] read 675000 sequences (100000045 bp) in 2.296 CPU sec, 2.503 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284828, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 333, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (353.19, 160.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 35, #reads = 1081866 mem = 536877517 peak = 1002225664
[M::process] read 674870 sequences (100000276 bp) in 2.510 CPU sec, 2.826 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284331, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (354.78, 161.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1149)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675242 reads in 177.411 CPU sec, 5.994 real sec
[M::mem_process_seqs] Processed 675000 reads in 111.487 CPU sec, 3.723 real sec
[M::process] read 675676 sequences (100000181 bp) in 2.170 CPU sec, 2.405 real sec...
sorting block 36, #reads = 1081517 mem = 536881873 peak = 1002225664
[M::process] read 677836 sequences (100000032 bp) in 2.438 CPU sec, 2.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284564, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (353.39, 162.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 272, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (333.90, 114.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675676 reads in 91.235 CPU sec, 3.060 real sec
sorting block 37, #reads = 1080990 mem = 536877983 peak = 1002225664
[M::process] read 677626 sequences (100000045 bp) in 2.511 CPU sec, 2.801 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 285706, 6, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (201, 210, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963)
[M::mem_pestat] mean and std.dev: (304.00, 123.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1217)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 313, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877)
[M::mem_pestat] mean and std.dev: (334.44, 154.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284694, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 338, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (358.63, 163.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677836 reads in 94.273 CPU sec, 3.311 real sec
[M::mem_process_seqs] Processed 674870 reads in 274.949 CPU sec, 9.424 real sec
[M::process] read 677144 sequences (100000220 bp) in 2.238 CPU sec, 2.454 real sec...
sorting block 38, #reads = 1081668 mem = 536876467 peak = 1002225664
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284780, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 315, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879)
[M::mem_pestat] mean and std.dev: (336.13, 153.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676818 sequences (100000195 bp) in 2.401 CPU sec, 2.647 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284544, 10, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 318, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (337.74, 153.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1098)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (301, 2324, 2324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6370)
[M::mem_pestat] mean and std.dev: (1483.10, 1033.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 8393)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 677626 reads in 169.547 CPU sec, 5.583 real sec
[M::mem_process_seqs] Processed 677144 reads in 111.022 CPU sec, 3.714 real sec
[M::process] read 676740 sequences (100000001 bp) in 1.866 CPU sec, 2.083 real sec...
sorting block 39, #reads = 1081227 mem = 536881276 peak = 1017954304
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285147, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 322, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (341.48, 155.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676528 sequences (100000147 bp) in 2.725 CPU sec, 3.142 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284595, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 325, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 896)
[M::mem_pestat] mean and std.dev: (343.67, 156.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676818 reads in 170.126 CPU sec, 5.602 real sec
[M::mem_process_seqs] Processed 676740 reads in 112.059 CPU sec, 3.704 real sec
sorting block 40, #reads = 1082148 mem = 536877858 peak = 1017954304
[M::process] read 676188 sequences (100000269 bp) in 1.915 CPU sec, 2.107 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284958, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 325, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 894)
[M::mem_pestat] mean and std.dev: (343.60, 155.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1118)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 41, #reads = 1082041 mem = 536879434 peak = 1017954304
[M::process] read 676126 sequences (100000206 bp) in 2.507 CPU sec, 3.036 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284875, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 327, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890)
[M::mem_pestat] mean and std.dev: (344.80, 154.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676528 reads in 166.582 CPU sec, 5.456 real sec
[M::mem_process_seqs] Processed 676188 reads in 109.251 CPU sec, 3.557 real sec
[M::process] read 675792 sequences (100000060 bp) in 2.621 CPU sec, 2.837 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284896, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 327, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (345.40, 155.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 42, #reads = 1082206 mem = 536878589 peak = 1017954304
[M::process] read 675758 sequences (100000141 bp) in 2.585 CPU sec, 2.922 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285025, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (349.51, 157.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676126 reads in 168.437 CPU sec, 5.539 real sec
[M::mem_process_seqs] Processed 675792 reads in 109.999 CPU sec, 3.595 real sec
[M::process] read 675496 sequences (100000253 bp) in 2.240 CPU sec, 2.448 real sec...
sorting block 43, #reads = 1081339 mem = 536878157 peak = 1038643200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284596, 8, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (350.58, 158.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675614 sequences (100000252 bp) in 2.441 CPU sec, 2.823 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284124, 9, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (352.77, 159.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675758 reads in 173.046 CPU sec, 5.657 real sec
[M::mem_process_seqs] Processed 675496 reads in 111.783 CPU sec, 3.628 real sec
[M::process] read 675446 sequences (100000213 bp) in 2.341 CPU sec, 2.475 real sec...
sorting block 44, #reads = 1081020 mem = 536879574 peak = 1038643200
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284431, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (353.06, 159.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675596 sequences (100000286 bp) in 2.402 CPU sec, 2.790 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284472, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (352.83, 158.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675614 reads in 172.227 CPU sec, 5.589 real sec
sorting block 45, #reads = 1081353 mem = 536878763 peak = 1041686528
[M::process] read 675646 sequences (100000016 bp) in 2.253 CPU sec, 2.516 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283921, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (352.98, 160.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675596 reads in 97.100 CPU sec, 3.243 real sec
[M::process] read 675648 sequences (100000089 bp) in 2.368 CPU sec, 2.638 real sec...
sorting block 46, #reads = 1081104 mem = 536877828 peak = 1041686528
[M::mem_process_seqs] Processed 675446 reads in 277.634 CPU sec, 9.104 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284240, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (351.29, 159.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1146)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283933, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (348.82, 158.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675712 sequences (100000271 bp) in 2.565 CPU sec, 2.914 real sec...
[M::mem_process_seqs] Processed 675646 reads in 188.325 CPU sec, 6.225 real sec
[M::mem_process_seqs] Processed 675648 reads in 112.111 CPU sec, 3.689 real sec
sorting block 47, #reads = 1081689 mem = 536879261 peak = 1041686528
[M::process] read 675720 sequences (100000099 bp) in 2.578 CPU sec, 2.712 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 284657, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (101, 273, 307)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (171.12, 97.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 925)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (349.16, 159.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675712 reads in 96.616 CPU sec, 3.354 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284408, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (348.85, 159.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 48, #reads = 1081121 mem = 536879625 peak = 1041686528
[M::process] read 675750 sequences (100000179 bp) in 2.859 CPU sec, 3.141 real sec...
[M::mem_process_seqs] Processed 675720 reads in 90.865 CPU sec, 3.036 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283767, 6, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (348.46, 158.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675708 sequences (100000093 bp) in 2.881 CPU sec, 3.084 real sec...
[M::mem_process_seqs] Processed 675750 reads in 92.263 CPU sec, 3.037 real sec
sorting block 49, #reads = 1081279 mem = 536878934 peak = 1041686528
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283884, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 327, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905)
[M::mem_pestat] mean and std.dev: (347.95, 158.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675564 sequences (100000056 bp) in 2.850 CPU sec, 3.061 real sec...
[M::process] read 675824 sequences (100000253 bp) in 2.215 CPU sec, 2.506 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284058, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (350.20, 159.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1137)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675564 reads in 96.726 CPU sec, 3.145 real sec
sorting block 50, #reads = 1081388 mem = 536878786 peak = 1041686528
[M::process] read 675380 sequences (100000217 bp) in 2.186 CPU sec, 2.362 real sec...
[M::mem_process_seqs] Processed 675708 reads in 250.471 CPU sec, 8.165 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284461, 1, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 329, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (350.25, 160.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283827, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (350.43, 159.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675486 sequences (100000102 bp) in 2.917 CPU sec, 3.255 real sec...
sorting block 51, #reads = 1081130 mem = 536880365 peak = 1058328576
[M::mem_process_seqs] Processed 675824 reads in 190.020 CPU sec, 6.166 real sec
[M::mem_process_seqs] Processed 675380 reads in 111.107 CPU sec, 3.595 real sec
[M::process] read 675462 sequences (100000176 bp) in 2.282 CPU sec, 2.695 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284230, 6, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (259, 259, 762)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1768)
[M::mem_pestat] mean and std.dev: (455.83, 310.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2271)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (350.74, 160.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 675486 reads in 94.454 CPU sec, 3.327 real sec
sorting block 52, #reads = 1080707 mem = 536877310 peak = 1058328576
[M::process] read 675304 sequences (100000105 bp) in 2.739 CPU sec, 3.006 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283985, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 331, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (351.57, 160.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1150)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675086 sequences (100000149 bp) in 2.368 CPU sec, 2.704 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284927, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (354.37, 161.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1156)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1081124 mem = 536879198 peak = 1058328576
[M::mem_process_seqs] Processed 675462 reads in 171.398 CPU sec, 5.664 real sec
[M::process] read 674990 sequences (100000021 bp) in 2.575 CPU sec, 2.775 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284688, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 334, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (354.40, 161.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675086 reads in 95.876 CPU sec, 3.192 real sec
sorting block 54, #reads = 1081085 mem = 536880562 peak = 1058328576
[M::process] read 674640 sequences (100000072 bp) in 2.136 CPU sec, 2.319 real sec...
[M::mem_process_seqs] Processed 675304 reads in 267.714 CPU sec, 8.953 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284206, 11, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 338, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929)
[M::mem_pestat] mean and std.dev: (357.22, 162.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (1134, 1596, 5466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14130)
[M::mem_pestat] mean and std.dev: (2431.18, 1985.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 18462)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284064, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 337, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (356.23, 161.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674648 sequences (100000267 bp) in 2.731 CPU sec, 2.903 real sec...
[M::mem_process_seqs] Processed 674990 reads in 177.850 CPU sec, 5.734 real sec
[M::mem_process_seqs] Processed 674640 reads in 105.468 CPU sec, 3.348 real sec
sorting block 55, #reads = 1081856 mem = 536881094 peak = 1058328576
[M::process] read 676642 sequences (100000220 bp) in 2.694 CPU sec, 2.880 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284261, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (358.17, 162.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 56, #reads = 1081786 mem = 536881017 peak = 1058328576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284618, 11, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 323, 447)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901)
[M::mem_pestat] mean and std.dev: (344.54, 159.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2270, 2270, 2402)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2006, 2666)
[M::mem_pestat] mean and std.dev: (2319.50, 63.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1874, 2798)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 674648 reads in 159.482 CPU sec, 5.125 real sec
[M::process] read 677928 sequences (100000121 bp) in 2.818 CPU sec, 3.002 real sec...
[M::mem_process_seqs] Processed 676642 reads in 106.884 CPU sec, 3.412 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284935, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 316, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (338.96, 158.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1126)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677602 sequences (100000271 bp) in 2.898 CPU sec, 3.086 real sec...
[M::mem_process_seqs] Processed 677928 reads in 86.226 CPU sec, 2.856 real sec
sorting block 57, #reads = 1081164 mem = 536880856 peak = 1058328576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284479, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 318, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (340.93, 159.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1132)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677218 sequences (100000117 bp) in 2.726 CPU sec, 2.926 real sec...
[M::mem_process_seqs] Processed 677602 reads in 90.288 CPU sec, 2.968 real sec
sorting block 58, #reads = 1083299 mem = 536880648 peak = 1058328576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284544, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 322, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (343.67, 159.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677062 sequences (100000099 bp) in 2.671 CPU sec, 2.830 real sec...
[M::mem_process_seqs] Processed 677218 reads in 92.598 CPU sec, 3.038 real sec
sorting block 59, #reads = 1081990 mem = 536878627 peak = 1058328576
[M::process] read 676818 sequences (100000027 bp) in 2.301 CPU sec, 2.446 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284752, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 324, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (345.00, 160.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1143)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676914 sequences (100000223 bp) in 1.834 CPU sec, 1.862 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284723, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 325, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (346.47, 160.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677062 reads in 161.955 CPU sec, 5.121 real sec
[M::process] read 676690 sequences (100000020 bp) in 1.755 CPU sec, 1.775 real sec...
[M::mem_process_seqs] Processed 676818 reads in 103.852 CPU sec, 3.269 real sec
sorting block 60, #reads = 1083087 mem = 536879782 peak = 1058328576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284778, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 326, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (347.90, 161.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1153)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676362 sequences (100000078 bp) in 2.852 CPU sec, 3.068 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284412, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921)
[M::mem_pestat] mean and std.dev: (348.60, 161.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676914 reads in 161.723 CPU sec, 5.156 real sec
[M::mem_process_seqs] Processed 676690 reads in 107.619 CPU sec, 3.402 real sec
[M::process] read 676192 sequences (100000028 bp) in 1.932 CPU sec, 1.978 real sec...
sorting block 61, #reads = 1082406 mem = 536881043 peak = 1058328576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284243, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (350.15, 160.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1159)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676592 sequences (100000268 bp) in 2.592 CPU sec, 2.735 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284208, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (350.00, 160.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675894 sequences (100000263 bp) in 1.694 CPU sec, 1.696 real sec...
[M::mem_process_seqs] Processed 676362 reads in 162.948 CPU sec, 5.184 real sec
sorting block 62, #reads = 1082555 mem = 536877189 peak = 1058328576
[M::mem_process_seqs] Processed 676192 reads in 106.143 CPU sec, 3.362 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284684, 4, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 323, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913)
[M::mem_pestat] mean and std.dev: (345.93, 160.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 63, #reads = 1082203 mem = 536879027 peak = 1058328576
[M::process] read 675812 sequences (100000070 bp) in 2.662 CPU sec, 2.818 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 283911, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (352.43, 161.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675722 sequences (100000135 bp) in 1.750 CPU sec, 1.758 real sec...
[M::mem_process_seqs] Processed 676592 reads in 161.191 CPU sec, 5.118 real sec
[M::mem_process_seqs] Processed 675894 reads in 105.035 CPU sec, 3.324 real sec
sorting block 64, #reads = 1082386 mem = 536879597 peak = 1058328576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 283808, 3, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (269, 352, 352)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (103, 518)
[M::mem_pestat] mean and std.dev: (299.88, 45.68)
[M::mem_pestat] low and high boundaries for proper pairs: (20, 601)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (352.37, 160.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675702 sequences (100000141 bp) in 2.570 CPU sec, 2.692 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284599, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 332, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925)
[M::mem_pestat] mean and std.dev: (352.12, 160.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1158)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675784 sequences (100000193 bp) in 1.750 CPU sec, 1.753 real sec...
[M::mem_process_seqs] Processed 675812 reads in 161.328 CPU sec, 5.124 real sec
[M::mem_process_seqs] Processed 675722 reads in 102.471 CPU sec, 3.239 real sec
sorting block 65, #reads = 1082101 mem = 536881838 peak = 1058328576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284109, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (352.16, 160.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1154)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675754 sequences (100000029 bp) in 2.688 CPU sec, 2.821 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 283706, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 330, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (349.55, 158.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675680 sequences (100000251 bp) in 1.760 CPU sec, 1.765 real sec...
[M::mem_process_seqs] Processed 675702 reads in 164.458 CPU sec, 5.229 real sec
[M::mem_process_seqs] Processed 675784 reads in 105.961 CPU sec, 3.346 real sec
sorting block 66, #reads = 1081931 mem = 536878628 peak = 1058328576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283936, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (348.66, 158.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675834 sequences (100000010 bp) in 2.678 CPU sec, 2.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283603, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (349.82, 159.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675898 sequences (100000110 bp) in 1.809 CPU sec, 1.815 real sec...
[M::mem_process_seqs] Processed 675754 reads in 164.031 CPU sec, 5.208 real sec
sorting block 67, #reads = 1080640 mem = 536877256 peak = 1058328576
[M::mem_process_seqs] Processed 675680 reads in 105.035 CPU sec, 3.316 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284294, 8, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (350.10, 159.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 68, #reads = 1080981 mem = 536880106 peak = 1072791552
[M::process] read 675884 sequences (100000169 bp) in 2.737 CPU sec, 2.880 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 284197, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (133, 446, 777)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2065)
[M::mem_pestat] mean and std.dev: (460.67, 350.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2709)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 919)
[M::mem_pestat] mean and std.dev: (349.18, 159.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675712 sequences (100000276 bp) in 1.800 CPU sec, 1.801 real sec...
[M::mem_process_seqs] Processed 675834 reads in 164.696 CPU sec, 5.225 real sec
[M::process] read 675680 sequences (100000095 bp) in 1.797 CPU sec, 1.804 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284284, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (348.07, 158.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675884 reads in 94.654 CPU sec, 3.211 real sec
sorting block 69, #reads = 1082231 mem = 536878987 peak = 1072791552
[M::mem_process_seqs] Processed 675898 reads in 230.155 CPU sec, 7.513 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284564, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (348.60, 158.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1140)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675348 sequences (100000231 bp) in 2.715 CPU sec, 2.888 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284227, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 328, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (347.55, 156.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675712 reads in 175.547 CPU sec, 5.584 real sec
sorting block 70, #reads = 1081594 mem = 536877442 peak = 1072791552
[M::mem_process_seqs] Processed 675680 reads in 104.275 CPU sec, 3.293 real sec
[M::process] read 675294 sequences (100000255 bp) in 2.237 CPU sec, 2.348 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284465, 11, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 331, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911)
[M::mem_pestat] mean and std.dev: (350.41, 157.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1139)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 423, 1909)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5485)
[M::mem_pestat] mean and std.dev: (879.91, 836.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7273)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 71, #reads = 1081971 mem = 536878822 peak = 1072791552
[M::process] read 675404 sequences (100000027 bp) in 2.232 CPU sec, 2.363 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283973, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 330, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (350.13, 157.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675348 reads in 161.708 CPU sec, 5.134 real sec
[M::process] read 675500 sequences (100000209 bp) in 1.825 CPU sec, 1.839 real sec...
[M::mem_process_seqs] Processed 675294 reads in 103.064 CPU sec, 3.248 real sec
sorting block 72, #reads = 1081268 mem = 536879535 peak = 1072791552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 283611, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 327, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892)
[M::mem_pestat] mean and std.dev: (346.74, 155.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675118 sequences (100000012 bp) in 2.697 CPU sec, 2.831 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284146, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 328, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 898)
[M::mem_pestat] mean and std.dev: (347.84, 156.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675404 reads in 159.519 CPU sec, 5.068 real sec
[M::process] read 674988 sequences (100000286 bp) in 1.933 CPU sec, 1.945 real sec...
[M::mem_process_seqs] Processed 675500 reads in 104.459 CPU sec, 3.291 real sec
sorting block 73, #reads = 1080433 mem = 536880196 peak = 1072791552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284698, 2, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 333, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (351.96, 158.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 256, 344)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708)
[M::mem_pestat] mean and std.dev: (227.71, 78.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 890)
[M::mem_pestat] skip orientation RR
[M::process] read 674872 sequences (100000141 bp) in 2.279 CPU sec, 2.392 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284703, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 335, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (353.70, 159.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1145)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675118 reads in 159.691 CPU sec, 5.082 real sec
[M::process] read 674788 sequences (100000133 bp) in 1.915 CPU sec, 1.919 real sec...
[M::mem_process_seqs] Processed 674988 reads in 104.915 CPU sec, 3.301 real sec
sorting block 74, #reads = 1081190 mem = 536880258 peak = 1072791552
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284904, 6, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (212, 366, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923)
[M::mem_pestat] mean and std.dev: (321.17, 116.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 338, 464)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (357.47, 161.56)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 680930 sequences (100000032 bp) in 2.770 CPU sec, 2.882 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 284014, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (231, 340, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (359.45, 163.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674872 reads in 159.712 CPU sec, 5.071 real sec
sorting block 75, #reads = 1082233 mem = 536879528 peak = 1072791552
[M::process] read 679628 sequences (100000130 bp) in 2.010 CPU sec, 2.039 real sec...
[M::mem_process_seqs] Processed 674788 reads in 104.098 CPU sec, 3.274 real sec
sorting block 76, #reads = 1080725 mem = 536878267 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286381, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 293, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (323.52, 157.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1097)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679116 sequences (100000022 bp) in 2.450 CPU sec, 2.604 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286261, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 302, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (330.89, 159.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1122)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678700 sequences (100000150 bp) in 1.870 CPU sec, 1.869 real sec...
[M::mem_process_seqs] Processed 680930 reads in 169.633 CPU sec, 5.394 real sec
[M::mem_process_seqs] Processed 679628 reads in 106.975 CPU sec, 3.375 real sec
sorting block 77, #reads = 1080915 mem = 536878779 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285906, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 309, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 908)
[M::mem_pestat] mean and std.dev: (335.80, 160.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678352 sequences (100000140 bp) in 2.725 CPU sec, 2.843 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286111, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 312, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904)
[M::mem_pestat] mean and std.dev: (336.63, 159.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679116 reads in 164.144 CPU sec, 5.205 real sec
[M::process] read 677890 sequences (100000114 bp) in 1.890 CPU sec, 1.890 real sec...
[M::mem_process_seqs] Processed 678700 reads in 104.759 CPU sec, 3.308 real sec
sorting block 78, #reads = 1082860 mem = 536879248 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285484, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 315, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (339.23, 160.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677488 sequences (100000277 bp) in 2.371 CPU sec, 2.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286225, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 318, 453)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (343.00, 162.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1170)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678352 reads in 164.593 CPU sec, 5.235 real sec
[M::process] read 677474 sequences (100000249 bp) in 1.930 CPU sec, 1.923 real sec...
sorting block 79, #reads = 1081568 mem = 536878027 peak = 1137594368
[M::mem_process_seqs] Processed 677890 reads in 106.957 CPU sec, 3.374 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285436, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 323, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (345.88, 163.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 80, #reads = 1081100 mem = 536880457 peak = 1137594368
[M::process] read 676978 sequences (100000078 bp) in 2.849 CPU sec, 3.021 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285172, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 325, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (348.16, 163.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (140, 176, 244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452)
[M::mem_pestat] mean and std.dev: (187.50, 57.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 556)
[M::mem_pestat] skip orientation RR
[M::process] read 676974 sequences (100000169 bp) in 1.890 CPU sec, 1.893 real sec...
[M::mem_process_seqs] Processed 677488 reads in 173.375 CPU sec, 5.498 real sec
[M::mem_process_seqs] Processed 677474 reads in 108.446 CPU sec, 3.429 real sec
sorting block 81, #reads = 1079928 mem = 536877062 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284813, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 329, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (350.17, 163.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676696 sequences (100000255 bp) in 2.473 CPU sec, 2.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285582, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 329, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (351.17, 163.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (369, 457, 1501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3765)
[M::mem_pestat] mean and std.dev: (888.46, 571.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4897)
[M::mem_pestat] skip orientation RR
[M::process] read 676528 sequences (100000205 bp) in 1.904 CPU sec, 1.910 real sec...
[M::mem_process_seqs] Processed 676978 reads in 166.228 CPU sec, 5.278 real sec
[M::mem_process_seqs] Processed 676974 reads in 107.415 CPU sec, 3.396 real sec
sorting block 82, #reads = 1081259 mem = 536884853 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285093, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 329, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (350.72, 162.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676026 sequences (100000089 bp) in 3.186 CPU sec, 3.347 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284952, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (354.75, 164.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676696 reads in 170.478 CPU sec, 5.417 real sec
[M::mem_process_seqs] Processed 676528 reads in 111.196 CPU sec, 3.501 real sec
[M::process] read 676290 sequences (100000178 bp) in 2.094 CPU sec, 2.128 real sec...
sorting block 83, #reads = 1080037 mem = 536879700 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284998, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (493, 616, 1112)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2350)
[M::mem_pestat] mean and std.dev: (566.92, 295.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2969)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (354.37, 163.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676400 sequences (100000073 bp) in 2.422 CPU sec, 2.546 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285456, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (353.36, 163.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676026 reads in 167.944 CPU sec, 5.348 real sec
[M::process] read 676218 sequences (100000012 bp) in 1.945 CPU sec, 1.956 real sec...
sorting block 84, #reads = 1079934 mem = 536880121 peak = 1137594368
[M::mem_process_seqs] Processed 676290 reads in 107.334 CPU sec, 3.387 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284957, 4, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (351.52, 162.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 546, 747)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1847)
[M::mem_pestat] mean and std.dev: (506.30, 377.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2397)
[M::mem_pestat] skip orientation RR
sorting block 85, #reads = 1078857 mem = 536882049 peak = 1137594368
[M::process] read 676180 sequences (100000147 bp) in 2.878 CPU sec, 3.049 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284466, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (351.63, 162.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676284 sequences (100000211 bp) in 1.462 CPU sec, 1.468 real sec...
[M::mem_process_seqs] Processed 676400 reads in 170.407 CPU sec, 5.422 real sec
[M::mem_process_seqs] Processed 676218 reads in 111.093 CPU sec, 3.525 real sec
sorting block 86, #reads = 1078969 mem = 536878766 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285087, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (351.78, 162.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676342 sequences (100000142 bp) in 2.491 CPU sec, 2.620 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285264, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (351.17, 161.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676186 sequences (100000188 bp) in 1.725 CPU sec, 1.734 real sec...
[M::mem_process_seqs] Processed 676180 reads in 166.100 CPU sec, 5.283 real sec
[M::mem_process_seqs] Processed 676284 reads in 107.288 CPU sec, 3.388 real sec
sorting block 87, #reads = 1079669 mem = 536879863 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284914, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (350.26, 161.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675818 sequences (100000224 bp) in 2.695 CPU sec, 2.802 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284565, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (349.63, 160.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676184 sequences (100000094 bp) in 1.900 CPU sec, 1.894 real sec...
[M::mem_process_seqs] Processed 676342 reads in 171.927 CPU sec, 5.460 real sec
[M::mem_process_seqs] Processed 676186 reads in 110.492 CPU sec, 3.493 real sec
sorting block 88, #reads = 1079943 mem = 536881038 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284584, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 334, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (353.15, 160.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676044 sequences (100000178 bp) in 2.567 CPU sec, 2.731 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284503, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (348.60, 159.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675662 sequences (100000109 bp) in 1.830 CPU sec, 1.834 real sec...
[M::mem_process_seqs] Processed 675818 reads in 169.787 CPU sec, 5.392 real sec
sorting block 89, #reads = 1078735 mem = 536877465 peak = 1137594368
[M::mem_process_seqs] Processed 676184 reads in 109.295 CPU sec, 3.456 real sec
sorting block 90, #reads = 1078354 mem = 536880595 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284572, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (348.25, 158.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676074 sequences (100000274 bp) in 2.765 CPU sec, 2.858 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284620, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 327, 450)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902)
[M::mem_pestat] mean and std.dev: (347.00, 156.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1128)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675994 sequences (100000194 bp) in 1.873 CPU sec, 1.877 real sec...
[M::mem_process_seqs] Processed 676044 reads in 169.411 CPU sec, 5.379 real sec
[M::mem_process_seqs] Processed 675662 reads in 108.150 CPU sec, 3.423 real sec
sorting block 91, #reads = 1077815 mem = 536881416 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285025, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 326, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (346.94, 157.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1135)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675782 sequences (100000056 bp) in 2.592 CPU sec, 2.744 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284526, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 328, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (348.61, 159.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1147)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676074 reads in 165.778 CPU sec, 5.276 real sec
[M::process] read 675934 sequences (100000180 bp) in 1.903 CPU sec, 1.908 real sec...
[M::mem_process_seqs] Processed 675994 reads in 108.067 CPU sec, 3.406 real sec
sorting block 92, #reads = 1079506 mem = 536878672 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285018, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 329, 456)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920)
[M::mem_pestat] mean and std.dev: (350.49, 160.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1152)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678458 sequences (100000133 bp) in 2.738 CPU sec, 2.837 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284714, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 330, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (352.74, 163.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1168)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681266 sequences (100000177 bp) in 1.897 CPU sec, 1.900 real sec...
[M::mem_process_seqs] Processed 675782 reads in 167.695 CPU sec, 5.313 real sec
[M::mem_process_seqs] Processed 675934 reads in 108.850 CPU sec, 3.439 real sec
sorting block 93, #reads = 1078104 mem = 536879363 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286229, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 314, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914)
[M::mem_pestat] mean and std.dev: (339.76, 162.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680088 sequences (100000234 bp) in 2.573 CPU sec, 2.701 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286828, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 291, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854)
[M::mem_pestat] mean and std.dev: (318.74, 153.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1072)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679314 sequences (100000233 bp) in 1.874 CPU sec, 1.880 real sec...
[M::mem_process_seqs] Processed 678458 reads in 175.210 CPU sec, 5.570 real sec
sorting block 94, #reads = 1077904 mem = 536885940 peak = 1137594368
[M::mem_process_seqs] Processed 681266 reads in 113.877 CPU sec, 3.604 real sec
sorting block 95, #reads = 1078175 mem = 536880116 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 287082, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (170, 1194, 1757)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4931)
[M::mem_pestat] mean and std.dev: (795.33, 627.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6518)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 300, 427)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873)
[M::mem_pestat] mean and std.dev: (325.27, 153.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1096)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 679092 sequences (100000216 bp) in 2.759 CPU sec, 2.863 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286021, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 304, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876)
[M::mem_pestat] mean and std.dev: (328.45, 154.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1099)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679318 sequences (100000246 bp) in 1.893 CPU sec, 1.896 real sec...
[M::mem_process_seqs] Processed 680088 reads in 170.753 CPU sec, 5.420 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 286473, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 308, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893)
[M::mem_pestat] mean and std.dev: (332.60, 156.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1121)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 679092 reads in 93.051 CPU sec, 2.948 real sec
[M::process] read 679114 sequences (100000264 bp) in 2.285 CPU sec, 2.298 real sec...
sorting block 96, #reads = 1079275 mem = 536881590 peak = 1137594368
[M::mem_process_seqs] Processed 679314 reads in 232.956 CPU sec, 7.388 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286950, 7, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (208, 307, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889)
[M::mem_pestat] mean and std.dev: (331.18, 155.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1116)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 97, #reads = 1078916 mem = 536884982 peak = 1137594368
[M::process] read 678970 sequences (100000025 bp) in 2.524 CPU sec, 2.630 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286406, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 308, 435)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (331.19, 154.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1113)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678056 sequences (100000161 bp) in 1.470 CPU sec, 1.472 real sec...
[M::mem_process_seqs] Processed 679318 reads in 193.553 CPU sec, 6.140 real sec
[M::mem_process_seqs] Processed 679114 reads in 115.683 CPU sec, 3.662 real sec
sorting block 98, #reads = 1078507 mem = 536884059 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286659, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 311, 441)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 903)
[M::mem_pestat] mean and std.dev: (334.97, 157.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1134)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677836 sequences (100000249 bp) in 2.768 CPU sec, 2.928 real sec...
[M::process] read 677650 sequences (100000294 bp) in 1.438 CPU sec, 1.439 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286358, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 317, 445)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907)
[M::mem_pestat] mean and std.dev: (339.03, 157.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1138)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678970 reads in 172.304 CPU sec, 5.479 real sec
[M::mem_process_seqs] Processed 678056 reads in 109.988 CPU sec, 3.494 real sec
sorting block 99, #reads = 1077474 mem = 536889104 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 286289, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (215, 320, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917)
[M::mem_pestat] mean and std.dev: (341.44, 159.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677496 sequences (100000179 bp) in 2.766 CPU sec, 2.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285486, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 320, 449)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915)
[M::mem_pestat] mean and std.dev: (342.29, 158.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1148)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676974 sequences (100000150 bp) in 1.903 CPU sec, 1.910 real sec...
[M::mem_process_seqs] Processed 677836 reads in 174.091 CPU sec, 5.524 real sec
[M::mem_process_seqs] Processed 677650 reads in 112.358 CPU sec, 3.553 real sec
sorting block 100, #reads = 1079523 mem = 536882055 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285681, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 324, 455)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (345.43, 160.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676764 sequences (100000120 bp) in 2.754 CPU sec, 2.883 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285902, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 328, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (349.26, 162.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676586 sequences (100000292 bp) in 1.822 CPU sec, 1.829 real sec...
[M::mem_process_seqs] Processed 677496 reads in 169.466 CPU sec, 5.384 real sec
[M::mem_process_seqs] Processed 676974 reads in 109.743 CPU sec, 3.467 real sec
sorting block 101, #reads = 1078856 mem = 536878289 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285475, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 331, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (351.20, 162.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 102, #reads = 1078819 mem = 536878981 peak = 1137594368
[M::process] read 676336 sequences (100000146 bp) in 2.599 CPU sec, 2.704 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285119, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 330, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944)
[M::mem_pestat] mean and std.dev: (351.52, 163.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1185)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676514 sequences (100000291 bp) in 1.897 CPU sec, 1.904 real sec...
[M::mem_process_seqs] Processed 676764 reads in 174.956 CPU sec, 5.563 real sec
[M::mem_process_seqs] Processed 676586 reads in 112.408 CPU sec, 3.550 real sec
sorting block 103, #reads = 1077442 mem = 536879453 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285201, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938)
[M::mem_pestat] mean and std.dev: (351.19, 163.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676382 sequences (100000186 bp) in 2.906 CPU sec, 3.027 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 285467, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (601, 1707, 2746)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7036)
[M::mem_pestat] mean and std.dev: (1417.18, 1064.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 9181)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 329, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (350.70, 163.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676128 sequences (100000105 bp) in 1.840 CPU sec, 1.839 real sec...
[M::mem_process_seqs] Processed 676336 reads in 170.747 CPU sec, 5.420 real sec
[M::mem_process_seqs] Processed 676514 reads in 106.745 CPU sec, 3.373 real sec
sorting block 104, #reads = 1078079 mem = 536879592 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285163, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (351.51, 163.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676214 sequences (100000078 bp) in 2.686 CPU sec, 2.840 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285000, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 330, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939)
[M::mem_pestat] mean and std.dev: (352.15, 163.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1178)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676382 reads in 168.633 CPU sec, 5.355 real sec
[M::process] read 676216 sequences (100000160 bp) in 1.915 CPU sec, 1.921 real sec...
[M::mem_process_seqs] Processed 676128 reads in 109.065 CPU sec, 3.440 real sec
sorting block 105, #reads = 1079670 mem = 536877780 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 285298, 3, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (259, 816, 2146)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5920)
[M::mem_pestat] mean and std.dev: (1002.75, 1408.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7807)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (351.37, 162.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 676174 sequences (100000292 bp) in 2.895 CPU sec, 3.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285536, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 333, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (354.68, 164.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676214 reads in 168.679 CPU sec, 5.352 real sec
[M::process] read 676130 sequences (100000175 bp) in 1.870 CPU sec, 1.866 real sec...
sorting block 106, #reads = 1079028 mem = 536881746 peak = 1137594368
[M::mem_process_seqs] Processed 676216 reads in 110.288 CPU sec, 3.481 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284914, 11, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 331, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (354.67, 166.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1191)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (121, 121, 194)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340)
[M::mem_pestat] mean and std.dev: (137.22, 30.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 413)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
sorting block 107, #reads = 1079352 mem = 536882104 peak = 1137594368
[M::process] read 675752 sequences (100000207 bp) in 2.777 CPU sec, 2.878 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285390, 5, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952)
[M::mem_pestat] mean and std.dev: (355.07, 166.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1195)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675754 sequences (100000286 bp) in 1.890 CPU sec, 1.893 real sec...
[M::mem_process_seqs] Processed 676174 reads in 165.207 CPU sec, 5.240 real sec
[M::mem_process_seqs] Processed 676130 reads in 107.562 CPU sec, 3.397 real sec
sorting block 108, #reads = 1079304 mem = 536879620 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284953, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 337, 471)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961)
[M::mem_pestat] mean and std.dev: (359.57, 168.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1206)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676054 sequences (100000258 bp) in 2.897 CPU sec, 3.056 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285561, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 338, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976)
[M::mem_pestat] mean and std.dev: (361.73, 170.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675752 reads in 161.572 CPU sec, 5.129 real sec
[M::process] read 676008 sequences (100000146 bp) in 1.982 CPU sec, 2.001 real sec...
[M::mem_process_seqs] Processed 675754 reads in 106.900 CPU sec, 3.374 real sec
sorting block 109, #reads = 1079797 mem = 536879478 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284867, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 336, 475)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975)
[M::mem_pestat] mean and std.dev: (360.84, 171.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679570 sequences (100000070 bp) in 2.725 CPU sec, 2.842 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285330, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 334, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (360.88, 172.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679682 sequences (100000172 bp) in 1.580 CPU sec, 1.578 real sec...
[M::mem_process_seqs] Processed 676054 reads in 172.373 CPU sec, 5.487 real sec
sorting block 110, #reads = 1078729 mem = 536880023 peak = 1137594368
[M::mem_process_seqs] Processed 676008 reads in 114.720 CPU sec, 3.638 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286068, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 306, 438)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900)
[M::mem_pestat] mean and std.dev: (333.25, 160.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1131)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678756 sequences (100000295 bp) in 2.877 CPU sec, 3.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286029, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 304, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (329.88, 157.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1114)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678314 sequences (100000008 bp) in 1.800 CPU sec, 1.798 real sec...
[M::mem_process_seqs] Processed 679570 reads in 177.894 CPU sec, 5.647 real sec
sorting block 111, #reads = 1073981 mem = 536878164 peak = 1137594368
[M::mem_process_seqs] Processed 679682 reads in 107.576 CPU sec, 3.409 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285889, 2, 18)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 308, 434)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (331.98, 155.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1106)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (189, 376, 577)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1353)
[M::mem_pestat] mean and std.dev: (496.71, 371.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1982)
[M::mem_pestat] skip orientation RR
sorting block 112, #reads = 1077289 mem = 536879459 peak = 1137594368
[M::process] read 678266 sequences (100000105 bp) in 2.876 CPU sec, 3.002 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285825, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (334.77, 155.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678756 reads in 164.859 CPU sec, 5.233 real sec
[M::process] read 678004 sequences (100000254 bp) in 1.855 CPU sec, 1.862 real sec...
[M::mem_process_seqs] Processed 678314 reads in 105.355 CPU sec, 3.324 real sec
sorting block 113, #reads = 1082288 mem = 536878452 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 286216, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (212, 313, 437)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887)
[M::mem_pestat] mean and std.dev: (334.83, 155.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1112)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677942 sequences (100000292 bp) in 2.550 CPU sec, 2.674 real sec...
[M::process] read 677424 sequences (100000275 bp) in 1.438 CPU sec, 1.447 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285756, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 314, 439)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891)
[M::mem_pestat] mean and std.dev: (335.88, 155.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1117)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 678266 reads in 167.133 CPU sec, 5.328 real sec
[M::mem_process_seqs] Processed 678004 reads in 105.830 CPU sec, 3.362 real sec
sorting block 114, #reads = 1079751 mem = 536878751 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285380, 3, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 317, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (339.63, 157.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677612 sequences (100000025 bp) in 3.100 CPU sec, 3.274 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285434, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 320, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912)
[M::mem_pestat] mean and std.dev: (342.23, 158.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1144)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677942 reads in 165.127 CPU sec, 5.247 real sec
[M::process] read 677258 sequences (100000263 bp) in 1.945 CPU sec, 1.982 real sec...
[M::mem_process_seqs] Processed 677424 reads in 104.918 CPU sec, 3.308 real sec
sorting block 115, #reads = 1080450 mem = 536881382 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285631, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (216, 322, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924)
[M::mem_pestat] mean and std.dev: (344.11, 160.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1160)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677364 sequences (100000181 bp) in 2.708 CPU sec, 2.834 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285619, 2, 18)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 325, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (345.62, 160.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (408, 619, 619)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (534.50, 131.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RR
[M::process] read 677144 sequences (100000158 bp) in 1.800 CPU sec, 1.802 real sec...
[M::mem_process_seqs] Processed 677612 reads in 169.531 CPU sec, 5.389 real sec
sorting block 116, #reads = 1080697 mem = 536878978 peak = 1137594368
[M::mem_process_seqs] Processed 677258 reads in 112.688 CPU sec, 3.561 real sec
sorting block 117, #reads = 1079390 mem = 536878789 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285279, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 322, 452)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 922)
[M::mem_pestat] mean and std.dev: (343.61, 158.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1157)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677060 sequences (100000069 bp) in 2.934 CPU sec, 3.104 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285582, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 325, 454)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926)
[M::mem_pestat] mean and std.dev: (345.32, 159.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1162)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676772 sequences (100000003 bp) in 1.830 CPU sec, 1.833 real sec...
[M::mem_process_seqs] Processed 677364 reads in 175.215 CPU sec, 5.562 real sec
[M::mem_process_seqs] Processed 677144 reads in 110.659 CPU sec, 3.506 real sec
sorting block 118, #reads = 1078896 mem = 536877747 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285099, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 327, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (347.67, 160.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676290 sequences (100000016 bp) in 2.309 CPU sec, 2.478 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285449, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 675978 sequences (100000122 bp) in 1.860 CPU sec, 1.858 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (221, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (349.42, 161.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677060 reads in 169.496 CPU sec, 5.390 real sec
[M::mem_process_seqs] Processed 676772 reads in 108.903 CPU sec, 3.450 real sec
sorting block 119, #reads = 1079566 mem = 536880324 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 284744, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 330, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (349.93, 160.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676236 sequences (100000162 bp) in 2.750 CPU sec, 2.847 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 284538, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 333, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (353.14, 161.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675996 sequences (100000178 bp) in 1.900 CPU sec, 1.896 real sec...
[M::mem_process_seqs] Processed 676290 reads in 175.423 CPU sec, 5.574 real sec
sorting block 120, #reads = 1079128 mem = 536877462 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285078, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 332, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (352.00, 161.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1183)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676146 sequences (100000181 bp) in 2.129 CPU sec, 2.130 real sec...
[M::mem_process_seqs] Processed 676236 reads in 101.590 CPU sec, 3.419 real sec
[M::mem_process_seqs] Processed 675978 reads in 243.038 CPU sec, 7.904 real sec
sorting block 121, #reads = 1079060 mem = 536880725 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284610, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (224, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (352.16, 161.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676080 sequences (100000142 bp) in 2.648 CPU sec, 2.792 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 285124, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (350.93, 161.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675996 reads in 193.090 CPU sec, 6.135 real sec
[M::process] read 676304 sequences (100000273 bp) in 1.958 CPU sec, 1.983 real sec...
[M::mem_process_seqs] Processed 676146 reads in 115.717 CPU sec, 3.657 real sec
sorting block 122, #reads = 1077852 mem = 536877819 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284602, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (350.54, 161.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676324 sequences (100000051 bp) in 2.744 CPU sec, 2.873 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285606, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (351.08, 162.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676112 sequences (100000122 bp) in 1.860 CPU sec, 1.861 real sec...
[M::mem_process_seqs] Processed 676080 reads in 173.399 CPU sec, 5.502 real sec
sorting block 123, #reads = 1078757 mem = 536878624 peak = 1137594368
[M::mem_process_seqs] Processed 676304 reads in 110.410 CPU sec, 3.497 real sec
sorting block 124, #reads = 1078275 mem = 536879140 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 284645, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 327, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930)
[M::mem_pestat] mean and std.dev: (349.24, 161.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1166)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675976 sequences (100000025 bp) in 2.601 CPU sec, 2.722 real sec...
[M::process] read 675942 sequences (100000092 bp) in 1.420 CPU sec, 1.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 285345, 6, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (351.82, 162.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676324 reads in 179.855 CPU sec, 5.731 real sec
[M::mem_process_seqs] Processed 676112 reads in 114.159 CPU sec, 3.641 real sec
sorting block 125, #reads = 1075728 mem = 536880882 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284920, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 460)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (351.01, 161.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (198, 1225, 1225)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3279)
[M::mem_pestat] mean and std.dev: (706.20, 520.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4306)
[M::mem_pestat] skip orientation RR
[M::process] read 676160 sequences (100000284 bp) in 2.365 CPU sec, 2.486 real sec...
[M::process] read 676246 sequences (100000242 bp) in 1.496 CPU sec, 1.504 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 285140, 4, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (351.68, 162.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675976 reads in 175.834 CPU sec, 5.603 real sec
[M::mem_process_seqs] Processed 675942 reads in 113.527 CPU sec, 3.622 real sec
sorting block 126, #reads = 1078176 mem = 536876603 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285285, 8, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 940)
[M::mem_pestat] mean and std.dev: (352.02, 163.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1179)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676814 sequences (100000022 bp) in 2.801 CPU sec, 2.934 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285272, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 331, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (353.92, 165.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1194)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 681918 sequences (100000021 bp) in 1.920 CPU sec, 1.916 real sec...
[M::mem_process_seqs] Processed 676160 reads in 179.289 CPU sec, 5.688 real sec
[M::mem_process_seqs] Processed 676246 reads in 116.612 CPU sec, 3.691 real sec
sorting block 127, #reads = 1076479 mem = 536878284 peak = 1137594368
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284697, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 327, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946)
[M::mem_pestat] mean and std.dev: (351.06, 165.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680728 sequences (100000087 bp) in 2.781 CPU sec, 2.883 real sec...
[M::process] read 680214 sequences (100000254 bp) in 1.930 CPU sec, 1.938 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 287368, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 285, 411)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 839)
[M::mem_pestat] mean and std.dev: (313.67, 150.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1053)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676814 reads in 197.904 CPU sec, 6.289 real sec
sorting block 128, #reads = 1075174 mem = 536877259 peak = 1137594368
[M::mem_process_seqs] Processed 681918 reads in 123.425 CPU sec, 3.915 real sec
sorting block 129, #reads = 1069180 mem = 536880955 peak = 1143681024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 286915, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (201, 292, 418)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852)
[M::mem_pestat] mean and std.dev: (318.73, 150.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1069)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679886 sequences (100000219 bp) in 2.941 CPU sec, 3.082 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286968, 0, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 298, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 867)
[M::mem_pestat] mean and std.dev: (323.21, 151.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1088)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (656, 671, 794)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (380, 1070)
[M::mem_pestat] mean and std.dev: (734.36, 89.18)
[M::mem_pestat] low and high boundaries for proper pairs: (242, 1208)
[M::mem_pestat] skip orientation RR
[M::process] read 679994 sequences (100000176 bp) in 1.880 CPU sec, 1.887 real sec...
[M::mem_process_seqs] Processed 680728 reads in 179.079 CPU sec, 5.679 real sec
[M::mem_process_seqs] Processed 680214 reads in 113.795 CPU sec, 3.601 real sec
sorting block 130, #reads = 1078304 mem = 536881832 peak = 1143681024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 286266, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (206, 302, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 878)
[M::mem_pestat] mean and std.dev: (326.76, 153.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1102)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 680724 sequences (100000233 bp) in 2.799 CPU sec, 2.881 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 286613, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (204, 299, 426)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870)
[M::mem_pestat] mean and std.dev: (324.35, 152.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1092)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679458 sequences (100000164 bp) in 1.930 CPU sec, 1.939 real sec...
[M::mem_process_seqs] Processed 679886 reads in 174.356 CPU sec, 5.525 real sec
[M::mem_process_seqs] Processed 679994 reads in 113.095 CPU sec, 3.581 real sec
sorting block 131, #reads = 1078383 mem = 536882273 peak = 1143681024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 286815, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (202, 295, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856)
[M::mem_pestat] mean and std.dev: (320.06, 149.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1074)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679718 sequences (100000019 bp) in 2.828 CPU sec, 2.949 real sec...
[M::process] read 678710 sequences (100000144 bp) in 1.910 CPU sec, 1.902 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286134, 3, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (209, 986, 986)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2540)
[M::mem_pestat] mean and std.dev: (696.40, 401.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3317)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (207, 304, 433)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885)
[M::mem_pestat] mean and std.dev: (328.40, 154.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1111)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 680724 reads in 182.301 CPU sec, 5.786 real sec
sorting block 132, #reads = 1078034 mem = 536883781 peak = 1143681024
[M::mem_process_seqs] Processed 679458 reads in 113.297 CPU sec, 3.587 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 286525, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (205, 301, 430)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880)
[M::mem_pestat] mean and std.dev: (327.33, 155.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1105)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 133, #reads = 1075949 mem = 536878884 peak = 1143681024
[M::process] read 678454 sequences (100000225 bp) in 2.924 CPU sec, 3.035 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 286415, 0, 12)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (314, 338, 396)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (150, 560)
[M::mem_pestat] mean and std.dev: (357.00, 33.22)
[M::mem_pestat] low and high boundaries for proper pairs: (68, 642)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (210, 311, 443)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 909)
[M::mem_pestat] mean and std.dev: (335.95, 157.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1142)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (199, 364, 1599)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4399)
[M::mem_pestat] mean and std.dev: (706.92, 840.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5799)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 678056 sequences (100000211 bp) in 1.916 CPU sec, 1.921 real sec...
[M::mem_process_seqs] Processed 679718 reads in 178.261 CPU sec, 5.649 real sec
[M::mem_process_seqs] Processed 678710 reads in 112.055 CPU sec, 3.553 real sec
sorting block 134, #reads = 1078656 mem = 536879792 peak = 1143681024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 286656, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (211, 314, 446)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 916)
[M::mem_pestat] mean and std.dev: (337.75, 157.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1151)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677264 sequences (100000199 bp) in 3.050 CPU sec, 3.206 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 285575, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (213, 318, 451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927)
[M::mem_pestat] mean and std.dev: (340.64, 159.05)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1165)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676890 sequences (100000211 bp) in 1.849 CPU sec, 1.850 real sec...
[M::mem_process_seqs] Processed 678454 reads in 180.252 CPU sec, 5.715 real sec
[M::mem_process_seqs] Processed 678056 reads in 118.441 CPU sec, 3.748 real sec
sorting block 135, #reads = 1077533 mem = 536877349 peak = 1143681024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 285783, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 327, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947)
[M::mem_pestat] mean and std.dev: (348.37, 161.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676918 sequences (100000161 bp) in 2.779 CPU sec, 2.875 real sec...
[M::process] read 676474 sequences (100000238 bp) in 1.450 CPU sec, 1.457 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 285046, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 326, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936)
[M::mem_pestat] mean and std.dev: (347.79, 160.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1175)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677264 reads in 183.970 CPU sec, 5.861 real sec
[M::mem_process_seqs] Processed 676890 reads in 118.823 CPU sec, 3.781 real sec
sorting block 136, #reads = 1076145 mem = 536882014 peak = 1143681024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 285436, 11, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (219, 326, 457)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (346.58, 159.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1171)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (2406, 2406, 2533)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (2152, 2787)
[M::mem_pestat] mean and std.dev: (2452.18, 61.09)
[M::mem_pestat] low and high boundaries for proper pairs: (2025, 2914)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 676342 sequences (100000087 bp) in 2.895 CPU sec, 3.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285077, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 328, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935)
[M::mem_pestat] mean and std.dev: (348.98, 159.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1173)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676310 sequences (100000294 bp) in 1.839 CPU sec, 1.841 real sec...
[M::mem_process_seqs] Processed 676918 reads in 183.540 CPU sec, 5.836 real sec
sorting block 137, #reads = 1076250 mem = 536882240 peak = 1143681024
[M::mem_process_seqs] Processed 676474 reads in 117.952 CPU sec, 3.736 real sec
sorting block 138, #reads = 1075861 mem = 536877835 peak = 1143681024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 285369, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 326, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934)
[M::mem_pestat] mean and std.dev: (348.39, 160.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1172)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676346 sequences (100000149 bp) in 2.879 CPU sec, 2.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 284980, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 329, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941)
[M::mem_pestat] mean and std.dev: (351.01, 162.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1181)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676304 sequences (100000081 bp) in 1.870 CPU sec, 1.871 real sec...
[M::mem_process_seqs] Processed 676342 reads in 173.841 CPU sec, 5.514 real sec
[M::mem_process_seqs] Processed 676310 reads in 109.756 CPU sec, 3.479 real sec
sorting block 139, #reads = 1077269 mem = 536883229 peak = 1143681024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 284427, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (220, 327, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (350.00, 162.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1184)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675976 sequences (100000133 bp) in 2.932 CPU sec, 3.047 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284671, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (222, 329, 462)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942)
[M::mem_pestat] mean and std.dev: (350.93, 161.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676048 sequences (100000107 bp) in 1.807 CPU sec, 1.810 real sec...
[M::mem_process_seqs] Processed 676346 reads in 176.933 CPU sec, 5.619 real sec
[M::mem_process_seqs] Processed 676304 reads in 111.825 CPU sec, 3.542 real sec
sorting block 140, #reads = 1078324 mem = 536879636 peak = 1143681024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 284616, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 329, 458)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928)
[M::mem_pestat] mean and std.dev: (350.09, 160.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1163)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675700 sequences (100000216 bp) in 2.986 CPU sec, 3.132 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 284584, 7, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (271, 362, 818)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1912)
[M::mem_pestat] mean and std.dev: (528.57, 426.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2459)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (223, 330, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 931)
[M::mem_pestat] mean and std.dev: (350.98, 161.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1167)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675420 sequences (100000273 bp) in 1.802 CPU sec, 1.806 real sec...
[M::mem_process_seqs] Processed 675976 reads in 175.435 CPU sec, 5.572 real sec
[M::mem_process_seqs] Processed 676048 reads in 110.960 CPU sec, 3.511 real sec
sorting block 141, #reads = 1078311 mem = 536878064 peak = 1143681024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 284809, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 331, 461)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933)
[M::mem_pestat] mean and std.dev: (352.44, 161.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1169)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675564 sequences (100000197 bp) in 2.821 CPU sec, 2.939 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 284685, 6, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 333, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937)
[M::mem_pestat] mean and std.dev: (354.32, 162.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1174)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675644 sequences (100000203 bp) in 1.630 CPU sec, 1.627 real sec...
[M::mem_process_seqs] Processed 675700 reads in 168.447 CPU sec, 5.358 real sec
sorting block 142, #reads = 1078911 mem = 536877615 peak = 1143681024
[M::mem_process_seqs] Processed 675420 reads in 108.363 CPU sec, 3.442 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 284675, 5, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (186, 186, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1209)
[M::mem_pestat] mean and std.dev: (301.31, 160.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1550)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943)
[M::mem_pestat] mean and std.dev: (355.70, 163.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1182)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 143, #reads = 1078695 mem = 536876903 peak = 1143681024
[M::process] read 675822 sequences (100000058 bp) in 3.157 CPU sec, 3.279 real sec...
[M::process] read 142206 sequences (20998752 bp) in 0.679 CPU sec, 0.683 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 285148, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (226, 335, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 949)
[M::mem_pestat] mean and std.dev: (357.16, 165.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1190)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675564 reads in 167.480 CPU sec, 5.345 real sec
[M::mem_process_seqs] Processed 675644 reads in 108.215 CPU sec, 3.457 real sec
sorting block 144, #reads = 1078913 mem = 536878096 peak = 1143681024
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 284721, 6, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (286, 286, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (286, 286)
[M::mem_pestat] mean and std.dev: (286.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (286, 286)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (225, 334, 467)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951)
[M::mem_pestat] mean and std.dev: (356.43, 165.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1193)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 59951, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (217, 320, 448)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910)
[M::mem_pestat] mean and std.dev: (341.99, 156.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1141)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675822 reads in 107.985 CPU sec, 3.511 real sec
[M::mem_process_seqs] Processed 142206 reads in 37.771 CPU sec, 1.222 real sec
sorting block 145, #reads = 1078121 mem = 536882678 peak = 1143681024
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 157106588
overall: 69886685184 mem 23402.645 user 565.352 sys 792.787 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.659_d0M-T1-TDNA-1_A23MHWWLT4_1.L002\tPU:L002\tSM:659_d0M-T1-TDNA-1_A23MHWWLT4_1\tLB:659_d0M-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 659_d0M-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 659_d0M-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 792.821 sec; CPU: 23968.000 sec
sorting block 146, #reads = 162280 mem = 81287983 peak = 1143681024
uniq header count 1
merging 147 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 8282684839
output reads: 157875278
bam_mem_sort: 147 calls 96.413 user 2.154 sys 100.499 real
bam_write: 147 calls 60.743 user 4.225 sys 104.202 real
execute: 1 calls 47.592 user 1.446 sys 847.610 real
merge_files: 1 calls 47.571 user 1.417 sys 54.669 real
parse_chunk: 2339 calls 106.100 user 26.500 sys 152.482 real
read_chunk: 6300 calls 53.603 user 40.836 sys 734.627 real
sort_block: 1 calls 34.479 user 40.236 sys 708.187 real
write_chunk: 3959 calls 47.198 user 1.086 sys 53.653 real
overall: 3189805056 mem 2183.502 user 93.074 sys 847.633 real
ls: cannot access '*.cram': No such file or directory