.command.log - Workdir - DAQ:SENTIEON_BWAMEM (1047_JG-T1-TDNA-1_A23MHWWLT4_1;L004;0002)

File Info

Filename: .command.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/8a/0f5d3238e8336e45bde2abfa3adca9/.command.log
Size: 131.2 KB
Task: DAQ:SENTIEON_BWAMEM (1047_JG-T1-TDNA-1_A23MHWWLT4_1;L004;0002)
Attempt
Content

  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/8a/0f5d3238e8336e45bde2abfa3adca9/.command.sh
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai
  Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/BWAIndex
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b9/e5d44c768f62c7836dec274a81691e/1047_JG-T1-TDNA-1_A23MHWWLT4_1/0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/b9/e5d44c768f62c7836dec274a81691e/1047_JG-T1-TDNA-1_A23MHWWLT4_1/0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/8a/0f5d3238e8336e45bde2abfa3adca9/.command.run
==> STAGING COMPLETE (7 inputs)

cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o 1047_JG-T1-TDNA-1_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1584.201 CPU sec, 57.009 real sec
[M::process] read 702664 sequences (100000063 bp) in 0.980 CPU sec, 0.985 real sec...
[M::process] read 702206 sequences (100000228 bp) in 1.204 CPU sec, 1.225 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292136, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 170, 700)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1844)
[M::mem_pestat] mean and std.dev: (349.40, 331.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2416)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (240.05, 114.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 789)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 701578 sequences (100000198 bp) in 1.234 CPU sec, 1.269 real sec...
[M::process] read 700576 sequences (100000300 bp) in 1.230 CPU sec, 1.267 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292369, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636)
[M::mem_pestat] mean and std.dev: (241.35, 115.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 797)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702664 reads in 137.521 CPU sec, 4.401 real sec
[M::process] read 700026 sequences (100000281 bp) in 1.300 CPU sec, 1.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291276, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 637)
[M::mem_pestat] mean and std.dev: (242.70, 116.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701578 reads in 71.305 CPU sec, 2.274 real sec
sorting block 0, #reads = 1095155 mem = 536884796 peak = 610643968
[M::mem_process_seqs] Processed 702206 reads in 169.604 CPU sec, 5.455 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 292066, 9, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 204, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522)
[M::mem_pestat] mean and std.dev: (212.90, 107.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 641)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (245.29, 117.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 811)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (136, 188, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1258)
[M::mem_pestat] mean and std.dev: (328.55, 318.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1632)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::process] read 699298 sequences (100000065 bp) in 1.628 CPU sec, 1.712 real sec...
[M::process] read 698894 sequences (100000246 bp) in 1.241 CPU sec, 1.249 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291406, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (248.10, 119.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700576 reads in 143.248 CPU sec, 4.580 real sec
sorting block 1, #reads = 1095113 mem = 536879286 peak = 911282176
[M::mem_process_seqs] Processed 700026 reads in 82.391 CPU sec, 2.639 real sec
sorting block 2, #reads = 1095020 mem = 536876527 peak = 922763264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291627, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::process] read 698510 sequences (100000171 bp) in 1.714 CPU sec, 1.818 real sec...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661)
[M::mem_pestat] mean and std.dev: (249.38, 120.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 829)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698332 sequences (100000209 bp) in 1.311 CPU sec, 1.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290763, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.36, 121.18)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699298 reads in 135.019 CPU sec, 4.317 real sec
[M::mem_process_seqs] Processed 698894 reads in 84.410 CPU sec, 2.709 real sec
sorting block 3, #reads = 1094106 mem = 536877647 peak = 922763264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291137, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.63, 120.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698638 sequences (100000215 bp) in 1.668 CPU sec, 1.809 real sec...
[M::process] read 698666 sequences (100000069 bp) in 1.284 CPU sec, 1.288 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290746, 5, 10)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 278, 1244)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3426)
[M::mem_pestat] mean and std.dev: (455.44, 479.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4517)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.00, 119.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (170, 270, 539)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1277)
[M::mem_pestat] mean and std.dev: (330.70, 188.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1646)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698510 reads in 134.611 CPU sec, 4.306 real sec
[M::mem_process_seqs] Processed 698332 reads in 83.977 CPU sec, 2.677 real sec
sorting block 4, #reads = 1093767 mem = 536880222 peak = 922763264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290926, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (249.60, 119.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698236 sequences (100000253 bp) in 1.854 CPU sec, 2.017 real sec...
[M::process] read 697842 sequences (100000186 bp) in 1.316 CPU sec, 1.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 291151, 4, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (151, 266, 1612)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4534)
[M::mem_pestat] mean and std.dev: (541.18, 561.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5995)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.45, 118.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698638 reads in 136.005 CPU sec, 4.351 real sec
sorting block 5, #reads = 1093319 mem = 536878259 peak = 922763264
[M::mem_process_seqs] Processed 698666 reads in 87.810 CPU sec, 2.816 real sec
sorting block 6, #reads = 1093742 mem = 536878668 peak = 922763264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290645, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.66, 119.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697314 sequences (100000114 bp) in 1.931 CPU sec, 2.072 real sec...
[M::process] read 696554 sequences (100000172 bp) in 1.647 CPU sec, 1.649 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290503, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.10, 120.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (385, 385, 543)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (69, 859)
[M::mem_pestat] mean and std.dev: (375.75, 111.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1017)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698236 reads in 153.673 CPU sec, 4.947 real sec
[M::mem_process_seqs] Processed 697842 reads in 97.522 CPU sec, 3.136 real sec
sorting block 7, #reads = 1092937 mem = 536881132 peak = 922763264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290490, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (252.47, 120.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 695798 sequences (100000245 bp) in 1.763 CPU sec, 1.876 real sec...
[M::process] read 696142 sequences (100000256 bp) in 1.370 CPU sec, 1.370 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290575, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 230, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (253.47, 120.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697314 reads in 143.772 CPU sec, 4.634 real sec
[M::mem_process_seqs] Processed 696554 reads in 87.933 CPU sec, 2.811 real sec
sorting block 8, #reads = 1093255 mem = 536878093 peak = 922763264
[M::process] read 695726 sequences (100000228 bp) in 1.770 CPU sec, 1.885 real sec...
[M::process] read 703876 sequences (100000118 bp) in 1.350 CPU sec, 1.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290861, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (239, 390, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (328.78, 142.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (161, 232, 334)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680)
[M::mem_pestat] mean and std.dev: (256.09, 122.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 853)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 696142 reads in 73.556 CPU sec, 2.332 real sec
sorting block 9, #reads = 1092784 mem = 536877627 peak = 922763264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 289986, 2, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (120, 166, 374)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882)
[M::mem_pestat] mean and std.dev: (213.90, 110.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1136)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 232, 333)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675)
[M::mem_pestat] mean and std.dev: (255.56, 121.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 846)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 289868, 5, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (244, 317, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (76, 496)
[M::mem_pestat] mean and std.dev: (287.30, 72.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 580)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (162, 233, 335)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681)
[M::mem_pestat] mean and std.dev: (257.37, 123.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 854)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 222, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518)
[M::mem_pestat] mean and std.dev: (238.60, 96.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 629)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 695798 reads in 204.675 CPU sec, 6.541 real sec
[M::process] read 702146 sequences (100000279 bp) in 1.576 CPU sec, 1.581 real sec...
[M::mem_process_seqs] Processed 695726 reads in 93.491 CPU sec, 2.994 real sec
sorting block 10, #reads = 1091872 mem = 536879019 peak = 922763264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292627, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 215, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (239.77, 115.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (134, 170, 372)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 848)
[M::mem_pestat] mean and std.dev: (197.91, 95.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1086)
[M::mem_pestat] skip orientation RR
[M::process] read 702086 sequences (100000013 bp) in 1.981 CPU sec, 2.145 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 292570, 11, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (243.46, 117.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (123, 126, 644)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1686)
[M::mem_pestat] mean and std.dev: (250.80, 343.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2207)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 703876 reads in 127.614 CPU sec, 4.071 real sec
[M::mem_process_seqs] Processed 702146 reads in 80.561 CPU sec, 2.547 real sec
[M::process] read 701404 sequences (100000184 bp) in 1.751 CPU sec, 1.793 real sec...
sorting block 11, #reads = 1093581 mem = 536877942 peak = 922763264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292125, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (244.24, 118.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700760 sequences (100000041 bp) in 1.906 CPU sec, 2.071 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291944, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 218, 1086)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2998)
[M::mem_pestat] mean and std.dev: (630.60, 781.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3954)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.31, 118.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 702086 reads in 128.041 CPU sec, 4.088 real sec
[M::process] read 699920 sequences (100000183 bp) in 1.425 CPU sec, 1.428 real sec...
sorting block 12, #reads = 1098086 mem = 536882302 peak = 922763264
[M::mem_process_seqs] Processed 701404 reads in 81.095 CPU sec, 2.567 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291956, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 222, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (247.78, 120.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 13, #reads = 1096624 mem = 536882364 peak = 922763264
[M::process] read 699374 sequences (100000057 bp) in 1.917 CPU sec, 2.053 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291193, 6, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (184, 273, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487)
[M::mem_pestat] mean and std.dev: (246.22, 55.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 588)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.61, 121.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700760 reads in 127.673 CPU sec, 4.082 real sec
[M::process] read 699270 sequences (100000031 bp) in 1.435 CPU sec, 1.441 real sec...
[M::mem_process_seqs] Processed 699920 reads in 81.357 CPU sec, 2.581 real sec
sorting block 14, #reads = 1096309 mem = 536878948 peak = 922763264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290967, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (251.18, 122.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 841)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698850 sequences (100000082 bp) in 2.220 CPU sec, 2.365 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291163, 18, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.67, 122.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (27, 42, 170)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456)
[M::mem_pestat] mean and std.dev: (82.18, 87.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 599)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 699374 reads in 129.198 CPU sec, 4.111 real sec
[M::process] read 698636 sequences (100000041 bp) in 1.420 CPU sec, 1.421 real sec...
[M::mem_process_seqs] Processed 699270 reads in 81.929 CPU sec, 2.592 real sec
sorting block 15, #reads = 1095537 mem = 536881898 peak = 922763264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291084, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.28, 121.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698822 sequences (100000011 bp) in 2.020 CPU sec, 2.170 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291251, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.37, 119.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (165, 234, 789)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2037)
[M::mem_pestat] mean and std.dev: (406.20, 342.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2661)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 698850 reads in 130.549 CPU sec, 4.167 real sec
[M::process] read 698908 sequences (100000070 bp) in 1.440 CPU sec, 1.438 real sec...
[M::mem_process_seqs] Processed 698636 reads in 82.273 CPU sec, 2.601 real sec
sorting block 16, #reads = 1094976 mem = 536878273 peak = 922763264
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291424, 10, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (247.79, 118.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 258, 258)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (90, 370)
[M::mem_pestat] mean and std.dev: (232.12, 48.78)
[M::mem_pestat] low and high boundaries for proper pairs: (34, 427)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 697848 sequences (100000114 bp) in 2.407 CPU sec, 2.578 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291130, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (247.90, 118.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698822 reads in 131.267 CPU sec, 4.177 real sec
sorting block 17, #reads = 1094723 mem = 536879472 peak = 922763264
[M::mem_process_seqs] Processed 698908 reads in 83.370 CPU sec, 2.636 real sec
[M::process] read 697764 sequences (100000129 bp) in 1.642 CPU sec, 1.675 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290685, 8, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (148, 257, 463)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1093)
[M::mem_pestat] mean and std.dev: (248.36, 124.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1408)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.64, 118.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 18, #reads = 1093644 mem = 536881844 peak = 998948864
[M::process] read 697548 sequences (100000058 bp) in 2.151 CPU sec, 2.301 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290446, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.39, 119.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697848 reads in 132.201 CPU sec, 4.226 real sec
[M::mem_process_seqs] Processed 697764 reads in 83.593 CPU sec, 2.643 real sec
[M::process] read 696824 sequences (100000025 bp) in 1.621 CPU sec, 1.663 real sec...
sorting block 19, #reads = 1093198 mem = 536880121 peak = 998948864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290924, 5, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 328)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (252.24, 121.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 835)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697042 sequences (100000299 bp) in 2.264 CPU sec, 2.408 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290355, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (253.19, 120.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697548 reads in 132.474 CPU sec, 4.227 real sec
[M::process] read 698842 sequences (100000080 bp) in 1.578 CPU sec, 1.613 real sec...
[M::mem_process_seqs] Processed 696824 reads in 84.286 CPU sec, 2.666 real sec
sorting block 20, #reads = 1093449 mem = 536876865 peak = 998948864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290231, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (160, 229, 330)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670)
[M::mem_pestat] mean and std.dev: (253.47, 121.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 840)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704310 sequences (100000143 bp) in 1.941 CPU sec, 2.063 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291255, 5, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.62, 121.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703862 sequences (100000188 bp) in 1.410 CPU sec, 1.407 real sec...
[M::mem_process_seqs] Processed 697042 reads in 133.558 CPU sec, 4.260 real sec
sorting block 21, #reads = 1091986 mem = 536878022 peak = 998948864
[M::mem_process_seqs] Processed 698842 reads in 84.471 CPU sec, 2.678 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 293026, 6, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633)
[M::mem_pestat] mean and std.dev: (238.87, 115.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 794)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 22, #reads = 1092012 mem = 536876546 peak = 998948864
[M::process] read 702590 sequences (100000138 bp) in 2.180 CPU sec, 2.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292795, 3, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634)
[M::mem_pestat] mean and std.dev: (239.93, 116.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 795)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 406, 1282)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3376)
[M::mem_pestat] mean and std.dev: (510.20, 424.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4423)
[M::mem_pestat] skip orientation RR
[M::process] read 701388 sequences (100000168 bp) in 1.460 CPU sec, 1.463 real sec...
[M::mem_process_seqs] Processed 704310 reads in 134.197 CPU sec, 4.272 real sec
[M::mem_process_seqs] Processed 703862 reads in 84.891 CPU sec, 2.691 real sec
sorting block 23, #reads = 1097778 mem = 536880160 peak = 998948864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292112, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (242.95, 117.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700578 sequences (100000024 bp) in 2.138 CPU sec, 2.262 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292049, 3, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 218, 238)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 314)
[M::mem_pestat] mean and std.dev: (226.11, 34.20)
[M::mem_pestat] low and high boundaries for proper pairs: (86, 363)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (245.47, 118.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 702590 reads in 136.062 CPU sec, 4.346 real sec
[M::process] read 700126 sequences (100000225 bp) in 1.447 CPU sec, 1.449 real sec...
[M::mem_process_seqs] Processed 701388 reads in 85.516 CPU sec, 2.710 real sec
sorting block 24, #reads = 1096372 mem = 536877680 peak = 998948864
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291686, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (247.86, 119.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 824)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699346 sequences (100000146 bp) in 2.491 CPU sec, 2.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 292014, 2, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (237, 329, 373)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (268.75, 83.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 781)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (249.24, 120.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 832)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 700578 reads in 126.487 CPU sec, 4.031 real sec
[M::mem_process_seqs] Processed 700126 reads in 80.640 CPU sec, 2.545 real sec
sorting block 25, #reads = 1096276 mem = 536876391 peak = 998948864
[M::process] read 699038 sequences (100000193 bp) in 1.705 CPU sec, 1.758 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291572, 12, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664)
[M::mem_pestat] mean and std.dev: (250.34, 120.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (478, 958, 1304)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2956)
[M::mem_pestat] mean and std.dev: (795.64, 535.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3782)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 698762 sequences (100000062 bp) in 2.025 CPU sec, 2.218 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291174, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665)
[M::mem_pestat] mean and std.dev: (250.90, 120.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 834)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699346 reads in 127.147 CPU sec, 4.061 real sec
sorting block 26, #reads = 1095326 mem = 536878629 peak = 998948864
[M::mem_process_seqs] Processed 699038 reads in 81.770 CPU sec, 2.587 real sec
[M::process] read 698712 sequences (100000249 bp) in 1.753 CPU sec, 1.848 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291351, 3, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (135, 151, 199)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 327)
[M::mem_pestat] mean and std.dev: (137.22, 44.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 391)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.40, 120.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 27, #reads = 1094606 mem = 536879246 peak = 1016508416
[M::process] read 698410 sequences (100000203 bp) in 1.988 CPU sec, 2.130 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 290913, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (153, 230, 843)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2223)
[M::mem_pestat] mean and std.dev: (375.45, 296.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2913)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (248.34, 118.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 821)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 698762 reads in 129.660 CPU sec, 4.137 real sec
[M::mem_process_seqs] Processed 698712 reads in 83.154 CPU sec, 2.624 real sec
[M::process] read 698200 sequences (100000006 bp) in 1.641 CPU sec, 1.694 real sec...
sorting block 28, #reads = 1094397 mem = 536879382 peak = 1016508416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291124, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.32, 118.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697784 sequences (100000168 bp) in 1.862 CPU sec, 1.973 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290534, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.70, 119.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698410 reads in 129.650 CPU sec, 4.139 real sec
[M::process] read 697688 sequences (100000134 bp) in 1.460 CPU sec, 1.483 real sec...
[M::mem_process_seqs] Processed 698200 reads in 83.515 CPU sec, 2.635 real sec
sorting block 29, #reads = 1093851 mem = 536884987 peak = 1016508416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290527, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (216, 532, 1278)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3402)
[M::mem_pestat] mean and std.dev: (583.00, 562.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4464)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.07, 118.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698040 sequences (100000228 bp) in 2.215 CPU sec, 2.321 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290297, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.29, 118.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697784 reads in 129.193 CPU sec, 4.105 real sec
[M::process] read 697480 sequences (100000143 bp) in 1.400 CPU sec, 1.403 real sec...
sorting block 30, #reads = 1092602 mem = 536878667 peak = 1016508416
[M::mem_process_seqs] Processed 697688 reads in 82.202 CPU sec, 2.600 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 290813, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (185, 304, 770)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1940)
[M::mem_pestat] mean and std.dev: (432.75, 385.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2525)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662)
[M::mem_pestat] mean and std.dev: (250.72, 120.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 830)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 31, #reads = 1091600 mem = 536878403 peak = 1016508416
[M::process] read 697618 sequences (100000185 bp) in 2.099 CPU sec, 2.276 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290599, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.26, 120.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698040 reads in 130.291 CPU sec, 4.165 real sec
[M::mem_process_seqs] Processed 697480 reads in 83.255 CPU sec, 2.628 real sec
[M::process] read 701388 sequences (100000020 bp) in 1.684 CPU sec, 1.752 real sec...
sorting block 32, #reads = 1091825 mem = 536883770 peak = 1016508416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290953, 4, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (109, 147, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1387)
[M::mem_pestat] mean and std.dev: (232.89, 181.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1813)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.48, 120.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702692 sequences (100000180 bp) in 2.069 CPU sec, 2.208 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291546, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (243.99, 117.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 244, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (151, 366)
[M::mem_pestat] mean and std.dev: (235.67, 42.99)
[M::mem_pestat] low and high boundaries for proper pairs: (64, 409)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697618 reads in 131.239 CPU sec, 4.180 real sec
[M::process] read 702332 sequences (100000016 bp) in 1.535 CPU sec, 1.554 real sec...
[M::mem_process_seqs] Processed 701388 reads in 84.367 CPU sec, 2.661 real sec
sorting block 33, #reads = 1092423 mem = 536882557 peak = 1016508416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292368, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 217, 314)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (241.57, 116.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 800)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701712 sequences (100000214 bp) in 2.024 CPU sec, 2.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292390, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 218, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.81, 116.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702692 reads in 127.821 CPU sec, 4.078 real sec
[M::mem_process_seqs] Processed 702332 reads in 81.821 CPU sec, 2.582 real sec
[M::process] read 701024 sequences (100000220 bp) in 1.536 CPU sec, 1.568 real sec...
sorting block 34, #reads = 1094579 mem = 536878194 peak = 1016508416
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292123, 6, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (244.52, 117.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700550 sequences (100000049 bp) in 2.331 CPU sec, 2.511 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 292177, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (246.84, 119.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 701712 reads in 126.590 CPU sec, 4.030 real sec
sorting block 35, #reads = 1097615 mem = 536877994 peak = 1016508416
[M::mem_process_seqs] Processed 701024 reads in 80.793 CPU sec, 2.552 real sec
[M::process] read 700008 sequences (100000130 bp) in 1.711 CPU sec, 1.761 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291620, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.69, 120.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (214, 254, 811)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2005)
[M::mem_pestat] mean and std.dev: (467.20, 403.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2602)
[M::mem_pestat] skip orientation RR
sorting block 36, #reads = 1097050 mem = 536877341 peak = 1058684928
[M::process] read 699480 sequences (100000122 bp) in 1.887 CPU sec, 1.996 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291497, 3, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 225, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666)
[M::mem_pestat] mean and std.dev: (249.75, 120.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 836)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 245, 2001)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5609)
[M::mem_pestat] mean and std.dev: (1058.70, 1365.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7413)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 700550 reads in 127.657 CPU sec, 4.083 real sec
[M::mem_process_seqs] Processed 700008 reads in 82.253 CPU sec, 2.593 real sec
[M::process] read 699184 sequences (100000051 bp) in 1.677 CPU sec, 1.748 real sec...
sorting block 37, #reads = 1094902 mem = 536878127 peak = 1058684928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 291173, 4, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (257, 953, 958)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2360)
[M::mem_pestat] mean and std.dev: (564.89, 379.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3061)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 226, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.43, 120.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 700586 sequences (100000045 bp) in 2.089 CPU sec, 2.181 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291168, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 225, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667)
[M::mem_pestat] mean and std.dev: (250.72, 121.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 837)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699480 reads in 128.792 CPU sec, 4.103 real sec
[M::process] read 699340 sequences (100000254 bp) in 1.493 CPU sec, 1.511 real sec...
[M::mem_process_seqs] Processed 699184 reads in 82.439 CPU sec, 2.608 real sec
sorting block 38, #reads = 1094423 mem = 536879264 peak = 1058684928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291661, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 220, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (243.99, 116.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 799)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698842 sequences (100000052 bp) in 1.951 CPU sec, 2.067 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291302, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (246.23, 117.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700586 reads in 130.022 CPU sec, 4.157 real sec
[M::mem_process_seqs] Processed 699340 reads in 82.748 CPU sec, 2.609 real sec
[M::process] read 698072 sequences (100000207 bp) in 1.524 CPU sec, 1.557 real sec...
sorting block 39, #reads = 1093459 mem = 536882625 peak = 1058684928
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 290514, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (247.11, 117.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 809)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 40, #reads = 1094267 mem = 536882313 peak = 1075064832
[M::process] read 697946 sequences (100000274 bp) in 2.085 CPU sec, 2.254 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290592, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (247.62, 116.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698842 reads in 129.639 CPU sec, 4.132 real sec
[M::process] read 697582 sequences (100000047 bp) in 1.528 CPU sec, 1.552 real sec...
[M::mem_process_seqs] Processed 698072 reads in 82.718 CPU sec, 2.608 real sec
sorting block 41, #reads = 1091401 mem = 536877926 peak = 1075064832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290662, 4, 19)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647)
[M::mem_pestat] mean and std.dev: (248.03, 116.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (203, 240, 603)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1403)
[M::mem_pestat] mean and std.dev: (327.44, 191.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1803)
[M::mem_pestat] skip orientation RR
[M::process] read 697396 sequences (100000168 bp) in 1.934 CPU sec, 2.051 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 290615, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (248.20, 116.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (357, 357, 1810)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4716)
[M::mem_pestat] mean and std.dev: (1005.73, 1078.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6169)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 697946 reads in 129.172 CPU sec, 4.113 real sec
[M::process] read 697510 sequences (100000218 bp) in 1.380 CPU sec, 1.382 real sec...
[M::mem_process_seqs] Processed 697582 reads in 82.206 CPU sec, 2.598 real sec
sorting block 42, #reads = 1093408 mem = 536879254 peak = 1075064832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 290720, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.58, 118.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 697368 sequences (100000024 bp) in 2.254 CPU sec, 2.356 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290915, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 326)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (251.23, 119.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 827)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697396 reads in 129.498 CPU sec, 4.110 real sec
[M::process] read 708660 sequences (100000151 bp) in 1.497 CPU sec, 1.502 real sec...
[M::mem_process_seqs] Processed 697510 reads in 82.392 CPU sec, 2.601 real sec
sorting block 43, #reads = 1091721 mem = 536878348 peak = 1075064832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290410, 8, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 228, 329)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669)
[M::mem_pestat] mean and std.dev: (252.91, 121.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 839)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 706560 sequences (100000131 bp) in 1.987 CPU sec, 2.111 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 294502, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (145, 201, 286)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568)
[M::mem_pestat] mean and std.dev: (222.94, 104.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 709)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704098 sequences (100000245 bp) in 1.390 CPU sec, 1.386 real sec...
[M::mem_process_seqs] Processed 697368 reads in 134.361 CPU sec, 4.279 real sec
sorting block 44, #reads = 1092188 mem = 536879358 peak = 1075064832
[M::mem_process_seqs] Processed 708660 reads in 87.798 CPU sec, 2.773 real sec
sorting block 45, #reads = 1093356 mem = 536883095 peak = 1093767168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293678, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 206, 298)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598)
[M::mem_pestat] mean and std.dev: (230.86, 110.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 748)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 562, 982)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2474)
[M::mem_pestat] mean and std.dev: (642.25, 621.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3220)
[M::mem_pestat] skip orientation RR
[M::process] read 702946 sequences (100000293 bp) in 2.272 CPU sec, 2.374 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 293089, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (236.34, 114.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (191, 252, 466)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (271.56, 134.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RR
[M::process] read 702226 sequences (100000138 bp) in 1.428 CPU sec, 1.436 real sec...
[M::mem_process_seqs] Processed 706560 reads in 132.678 CPU sec, 4.211 real sec
[M::mem_process_seqs] Processed 704098 reads in 82.864 CPU sec, 2.622 real sec
sorting block 46, #reads = 1096631 mem = 536878350 peak = 1093767168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 292914, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 312)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632)
[M::mem_pestat] mean and std.dev: (240.25, 116.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 792)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701370 sequences (100000123 bp) in 1.973 CPU sec, 2.078 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292384, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 216, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642)
[M::mem_pestat] mean and std.dev: (242.92, 118.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702946 reads in 130.588 CPU sec, 4.160 real sec
[M::process] read 700910 sequences (100000220 bp) in 1.428 CPU sec, 1.428 real sec...
[M::mem_process_seqs] Processed 702226 reads in 83.383 CPU sec, 2.633 real sec
sorting block 47, #reads = 1096270 mem = 536879073 peak = 1093767168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 291950, 6, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 218, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646)
[M::mem_pestat] mean and std.dev: (244.46, 118.84)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 810)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 700088 sequences (100000093 bp) in 2.301 CPU sec, 2.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 291629, 6, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (234, 261, 822)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1998)
[M::mem_pestat] mean and std.dev: (346.08, 277.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2586)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 220, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655)
[M::mem_pestat] mean and std.dev: (246.23, 120.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 701370 reads in 131.143 CPU sec, 4.164 real sec
[M::process] read 699658 sequences (100000218 bp) in 1.440 CPU sec, 1.436 real sec...
sorting block 48, #reads = 1094813 mem = 536877991 peak = 1093767168
[M::mem_process_seqs] Processed 700910 reads in 83.762 CPU sec, 2.645 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291327, 6, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.45, 120.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (109, 182, 231)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475)
[M::mem_pestat] mean and std.dev: (150.78, 77.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 597)
[M::mem_pestat] skip orientation RR
sorting block 49, #reads = 1094054 mem = 536881980 peak = 1093767168
[M::process] read 699084 sequences (100000089 bp) in 2.184 CPU sec, 2.366 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291243, 4, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660)
[M::mem_pestat] mean and std.dev: (248.90, 120.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 828)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700088 reads in 134.784 CPU sec, 4.305 real sec
[M::mem_process_seqs] Processed 699658 reads in 86.232 CPU sec, 2.725 real sec
[M::process] read 699356 sequences (100000164 bp) in 1.676 CPU sec, 1.736 real sec...
sorting block 50, #reads = 1092174 mem = 536878175 peak = 1093767168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291299, 5, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (247.99, 119.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (197, 302, 442)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932)
[M::mem_pestat] mean and std.dev: (294.33, 105.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1177)
[M::mem_pestat] skip orientation RR
[M::process] read 698466 sequences (100000032 bp) in 2.187 CPU sec, 2.318 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 291119, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.41, 119.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699084 reads in 134.709 CPU sec, 4.292 real sec
[M::mem_process_seqs] Processed 699356 reads in 86.579 CPU sec, 2.729 real sec
[M::process] read 698524 sequences (100000028 bp) in 1.606 CPU sec, 1.627 real sec...
sorting block 51, #reads = 1092271 mem = 536878994 peak = 1093767168
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291449, 2, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.60, 119.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (558, 1193, 2005)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4899)
[M::mem_pestat] mean and std.dev: (1146.55, 747.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6346)
[M::mem_pestat] skip orientation RR
[M::process] read 698270 sequences (100000117 bp) in 1.994 CPU sec, 2.116 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 290672, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 224, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (248.28, 118.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698254 sequences (100000171 bp) in 1.380 CPU sec, 1.381 real sec...
[M::mem_process_seqs] Processed 698466 reads in 135.020 CPU sec, 4.300 real sec
sorting block 52, #reads = 1091780 mem = 536878508 peak = 1093767168
[M::mem_process_seqs] Processed 698524 reads in 85.642 CPU sec, 2.710 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290514, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.42, 119.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698158 sequences (100000177 bp) in 2.333 CPU sec, 2.476 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 290881, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.64, 119.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 698454 sequences (100000166 bp) in 1.430 CPU sec, 1.432 real sec...
[M::mem_process_seqs] Processed 698270 reads in 135.201 CPU sec, 4.290 real sec
sorting block 53, #reads = 1091469 mem = 536884121 peak = 1093767168
[M::mem_process_seqs] Processed 698254 reads in 85.412 CPU sec, 2.705 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 291052, 3, 14)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (202, 220, 1892)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5272)
[M::mem_pestat] mean and std.dev: (885.45, 921.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6962)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.34, 120.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 208, 249)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495)
[M::mem_pestat] mean and std.dev: (159.09, 54.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 618)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 54, #reads = 1091540 mem = 536882765 peak = 1139589120
[M::process] read 710820 sequences (100000088 bp) in 1.983 CPU sec, 2.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291307, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 323)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653)
[M::mem_pestat] mean and std.dev: (248.99, 119.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 818)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698158 reads in 136.080 CPU sec, 4.339 real sec
[M::process] read 709416 sequences (100000078 bp) in 1.503 CPU sec, 1.510 real sec...
[M::mem_process_seqs] Processed 698454 reads in 87.315 CPU sec, 2.756 real sec
sorting block 55, #reads = 1090966 mem = 536878728 peak = 1139589120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 295027, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (143, 197, 280)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554)
[M::mem_pestat] mean and std.dev: (218.53, 101.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 691)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 707276 sequences (100000149 bp) in 2.327 CPU sec, 2.448 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 295191, 3, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 200, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567)
[M::mem_pestat] mean and std.dev: (222.31, 104.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 708)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705732 sequences (100000091 bp) in 1.450 CPU sec, 1.455 real sec...
[M::mem_process_seqs] Processed 710820 reads in 144.675 CPU sec, 4.600 real sec
[M::mem_process_seqs] Processed 709416 reads in 92.777 CPU sec, 2.936 real sec
sorting block 56, #reads = 1092654 mem = 536880786 peak = 1139589120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293949, 2, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (149, 608, 1174)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3224)
[M::mem_pestat] mean and std.dev: (870.80, 923.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4563)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 204, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588)
[M::mem_pestat] mean and std.dev: (227.95, 108.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 705256 sequences (100000029 bp) in 2.237 CPU sec, 2.420 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293730, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 300)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604)
[M::mem_pestat] mean and std.dev: (232.25, 111.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 756)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 707276 reads in 141.869 CPU sec, 4.521 real sec
[M::process] read 704554 sequences (100000140 bp) in 1.499 CPU sec, 1.511 real sec...
sorting block 57, #reads = 1093933 mem = 536879709 peak = 1139589120
[M::mem_process_seqs] Processed 705732 reads in 90.543 CPU sec, 2.860 real sec
sorting block 58, #reads = 1093456 mem = 536882207 peak = 1139589120
[M::process] read 703566 sequences (100000282 bp) in 2.490 CPU sec, 2.662 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 293263, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 210, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (236.22, 114.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704554 reads in 76.565 CPU sec, 2.413 real sec
[M::process] read 702654 sequences (100000114 bp) in 1.588 CPU sec, 1.608 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 293165, 6, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (150, 1043, 1173)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3219)
[M::mem_pestat] mean and std.dev: (680.83, 551.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 4242)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 209, 303)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611)
[M::mem_pestat] mean and std.dev: (234.27, 113.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 765)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293251, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (239.50, 115.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 705256 reads in 213.465 CPU sec, 6.788 real sec
[M::mem_process_seqs] Processed 703566 reads in 101.020 CPU sec, 3.210 real sec
[M::process] read 701260 sequences (100000131 bp) in 1.629 CPU sec, 1.666 real sec...
sorting block 59, #reads = 1094326 mem = 536879186 peak = 1139589120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292300, 9, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 217, 316)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (242.75, 117.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 60, #reads = 1093793 mem = 536879825 peak = 1139589120
[M::process] read 700036 sequences (100000155 bp) in 2.032 CPU sec, 2.169 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 292117, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (245.36, 119.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 814)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702654 reads in 138.003 CPU sec, 4.398 real sec
[M::process] read 699516 sequences (100000143 bp) in 1.390 CPU sec, 1.398 real sec...
[M::mem_process_seqs] Processed 701260 reads in 88.158 CPU sec, 2.785 real sec
sorting block 61, #reads = 1091401 mem = 536878031 peak = 1139589120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 291472, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (155, 221, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650)
[M::mem_pestat] mean and std.dev: (246.22, 118.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699116 sequences (100000023 bp) in 2.325 CPU sec, 2.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291221, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (246.75, 118.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699136 sequences (100000161 bp) in 1.420 CPU sec, 1.421 real sec...
[M::mem_process_seqs] Processed 700036 reads in 136.654 CPU sec, 4.341 real sec
[M::mem_process_seqs] Processed 699516 reads in 86.988 CPU sec, 2.753 real sec
sorting block 62, #reads = 1092195 mem = 536877110 peak = 1139589120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291365, 2, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652)
[M::mem_pestat] mean and std.dev: (247.92, 119.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 817)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (184, 343, 967)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2533)
[M::mem_pestat] mean and std.dev: (655.08, 644.29)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3316)
[M::mem_pestat] skip orientation RR
[M::process] read 698420 sequences (100000203 bp) in 2.149 CPU sec, 2.275 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291158, 8, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.70, 119.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699116 reads in 135.497 CPU sec, 4.314 real sec
[M::process] read 698066 sequences (100000263 bp) in 1.659 CPU sec, 1.677 real sec...
[M::mem_process_seqs] Processed 699136 reads in 86.897 CPU sec, 2.737 real sec
sorting block 63, #reads = 1091668 mem = 536878879 peak = 1139589120
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 291343, 5, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (140, 347, 380)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860)
[M::mem_pestat] mean and std.dev: (246.91, 107.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1100)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.46, 119.78)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 698574 sequences (100000284 bp) in 2.491 CPU sec, 2.676 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290700, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659)
[M::mem_pestat] mean and std.dev: (250.07, 120.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 826)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 698420 reads in 132.434 CPU sec, 4.223 real sec
sorting block 64, #reads = 1091338 mem = 536879722 peak = 1139589120
[M::mem_process_seqs] Processed 698066 reads in 84.907 CPU sec, 2.678 real sec
[M::process] read 707424 sequences (100000008 bp) in 1.736 CPU sec, 1.798 real sec...
sorting block 65, #reads = 1091495 mem = 536882493 peak = 1166798848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 291009, 8, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.79, 119.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 707418 sequences (100000060 bp) in 1.849 CPU sec, 1.959 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293763, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 204, 295)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591)
[M::mem_pestat] mean and std.dev: (228.35, 108.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 739)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 706110 sequences (100000292 bp) in 1.400 CPU sec, 1.398 real sec...
[M::mem_process_seqs] Processed 698574 reads in 141.502 CPU sec, 4.507 real sec
[M::mem_process_seqs] Processed 707424 reads in 91.277 CPU sec, 2.890 real sec
sorting block 66, #reads = 1088803 mem = 536879038 peak = 1166798848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 293875, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (147, 204, 296)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594)
[M::mem_pestat] mean and std.dev: (228.94, 109.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 743)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 704368 sequences (100000109 bp) in 2.288 CPU sec, 2.418 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 293179, 7, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (128, 262, 561)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1427)
[M::mem_pestat] mean and std.dev: (329.00, 223.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1860)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (232.47, 111.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 760)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 703668 sequences (100000014 bp) in 1.410 CPU sec, 1.414 real sec...
[M::mem_process_seqs] Processed 707418 reads in 131.873 CPU sec, 4.185 real sec
sorting block 67, #reads = 1097123 mem = 536880743 peak = 1166798848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 293202, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (208, 282, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (20, 490)
[M::mem_pestat] mean and std.dev: (232.33, 67.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 584)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 211, 306)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618)
[M::mem_pestat] mean and std.dev: (235.90, 113.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 774)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 702910 sequences (100000162 bp) in 1.516 CPU sec, 1.519 real sec...
[M::mem_process_seqs] Processed 704368 reads in 76.658 CPU sec, 2.667 real sec
[M::mem_process_seqs] Processed 706110 reads in 180.720 CPU sec, 5.971 real sec
sorting block 68, #reads = 1097071 mem = 536876886 peak = 1166798848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 293044, 4, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 214, 311)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631)
[M::mem_pestat] mean and std.dev: (238.97, 114.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 791)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 703232 sequences (100000117 bp) in 2.132 CPU sec, 2.243 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292863, 4, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.64, 116.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703668 reads in 146.534 CPU sec, 4.653 real sec
[M::mem_process_seqs] Processed 702910 reads in 88.461 CPU sec, 2.789 real sec
[M::process] read 702422 sequences (100000120 bp) in 1.599 CPU sec, 1.637 real sec...
sorting block 69, #reads = 1097886 mem = 536880635 peak = 1166798848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292726, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 215, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.44, 116.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 701514 sequences (100000026 bp) in 1.929 CPU sec, 2.065 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 292432, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.37, 116.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 703232 reads in 131.573 CPU sec, 4.188 real sec
[M::process] read 701956 sequences (100000004 bp) in 1.506 CPU sec, 1.508 real sec...
sorting block 70, #reads = 1095708 mem = 536884517 peak = 1166798848
[M::mem_process_seqs] Processed 702422 reads in 83.751 CPU sec, 2.645 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 292262, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (154, 219, 317)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (243.62, 117.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 71, #reads = 1095470 mem = 536883520 peak = 1166798848
[M::process] read 700054 sequences (100000147 bp) in 2.300 CPU sec, 2.409 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292249, 10, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (153, 217, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (242.00, 116.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (130, 622, 1703)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4849)
[M::mem_pestat] mean and std.dev: (763.50, 665.76)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 6422)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_process_seqs] Processed 701514 reads in 132.023 CPU sec, 4.191 real sec
[M::process] read 700276 sequences (100000281 bp) in 1.637 CPU sec, 1.647 real sec...
[M::mem_process_seqs] Processed 701956 reads in 84.673 CPU sec, 2.668 real sec
sorting block 72, #reads = 1094987 mem = 536877641 peak = 1166798848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 291780, 3, 16)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 322)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654)
[M::mem_pestat] mean and std.dev: (247.58, 119.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 820)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (196, 388, 575)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1333)
[M::mem_pestat] mean and std.dev: (415.06, 245.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1712)
[M::mem_pestat] skip orientation RR
[M::process] read 699722 sequences (100000172 bp) in 2.294 CPU sec, 2.488 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292031, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.20, 119.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 700054 reads in 134.649 CPU sec, 4.288 real sec
[M::mem_process_seqs] Processed 700276 reads in 86.934 CPU sec, 2.740 real sec
[M::process] read 699226 sequences (100000140 bp) in 1.860 CPU sec, 1.924 real sec...
sorting block 73, #reads = 1093270 mem = 536878291 peak = 1166798848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290986, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (245.82, 117.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699268 sequences (100000089 bp) in 2.352 CPU sec, 2.480 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 291576, 5, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (111, 149, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639)
[M::mem_pestat] mean and std.dev: (166.27, 71.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 815)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648)
[M::mem_pestat] mean and std.dev: (246.85, 118.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 812)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 699722 reads in 138.102 CPU sec, 4.393 real sec
[M::mem_process_seqs] Processed 699226 reads in 87.609 CPU sec, 2.761 real sec
[M::process] read 698832 sequences (100000231 bp) in 1.841 CPU sec, 1.884 real sec...
sorting block 74, #reads = 1092036 mem = 536879163 peak = 1166798848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290922, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651)
[M::mem_pestat] mean and std.dev: (247.41, 119.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 816)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 699118 sequences (100000046 bp) in 2.224 CPU sec, 2.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 290612, 7, 11)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (229, 281, 643)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1471)
[M::mem_pestat] mean and std.dev: (349.60, 224.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1885)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.62, 118.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (137, 227, 242)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452)
[M::mem_pestat] mean and std.dev: (190.10, 59.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 557)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699268 reads in 139.017 CPU sec, 4.434 real sec
sorting block 75, #reads = 1091442 mem = 536877789 peak = 1166798848
[M::mem_process_seqs] Processed 698832 reads in 90.113 CPU sec, 2.845 real sec
[M::process] read 711610 sequences (100000050 bp) in 1.941 CPU sec, 2.010 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 290665, 6, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (199, 224, 234)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (129, 304)
[M::mem_pestat] mean and std.dev: (226.22, 32.01)
[M::mem_pestat] low and high boundaries for proper pairs: (94, 354)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 76, #reads = 1088825 mem = 536880903 peak = 1166798848
[M::mem_pestat] (25, 50, 75) percentile: (157, 223, 321)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649)
[M::mem_pestat] mean and std.dev: (247.36, 118.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 813)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 709864 sequences (100000032 bp) in 2.418 CPU sec, 2.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 295121, 5, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (142, 196, 279)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553)
[M::mem_pestat] mean and std.dev: (218.09, 101.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 690)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 699118 reads in 150.606 CPU sec, 4.784 real sec
[M::process] read 708002 sequences (100000234 bp) in 1.530 CPU sec, 1.527 real sec...
[M::mem_process_seqs] Processed 711610 reads in 95.573 CPU sec, 3.017 real sec
sorting block 77, #reads = 1084859 mem = 536880183 peak = 1166798848
[M::process] read 706386 sequences (100000235 bp) in 2.191 CPU sec, 2.346 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 294214, 7, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (146, 203, 294)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590)
[M::mem_pestat] mean and std.dev: (227.78, 108.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 738)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 705164 sequences (100000096 bp) in 1.560 CPU sec, 1.551 real sec...
[M::mem_process_seqs] Processed 708002 reads in 82.504 CPU sec, 2.620 real sec
sorting block 78, #reads = 1094491 mem = 536886906 peak = 1166798848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 295104, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (144, 200, 287)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573)
[M::mem_pestat] mean and std.dev: (223.46, 105.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 716)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293416, 8, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (148, 207, 301)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607)
[M::mem_pestat] mean and std.dev: (232.63, 111.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 760)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 709864 reads in 220.507 CPU sec, 7.022 real sec
[M::process] read 703992 sequences (100000084 bp) in 1.785 CPU sec, 1.787 real sec...
[M::mem_process_seqs] Processed 706386 reads in 104.767 CPU sec, 3.327 real sec
sorting block 79, #reads = 1092871 mem = 536877803 peak = 1166798848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 293333, 3, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (187, 232, 255)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (51, 391)
[M::mem_pestat] mean and std.dev: (202.22, 44.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 459)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (149, 210, 305)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617)
[M::mem_pestat] mean and std.dev: (235.48, 113.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 773)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 704116 sequences (100000093 bp) in 2.789 CPU sec, 2.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 293001, 6, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 213, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628)
[M::mem_pestat] mean and std.dev: (238.88, 115.41)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 787)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 705164 reads in 139.413 CPU sec, 4.430 real sec
[M::mem_process_seqs] Processed 703992 reads in 88.727 CPU sec, 2.797 real sec
[M::process] read 703240 sequences (100000043 bp) in 1.808 CPU sec, 1.867 real sec...
sorting block 80, #reads = 1092140 mem = 536880783 peak = 1166798848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 293030, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (150, 213, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630)
[M::mem_pestat] mean and std.dev: (239.00, 116.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 790)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 702790 sequences (100000203 bp) in 2.083 CPU sec, 2.231 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 292554, 5, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 313)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (240.69, 116.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 796)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 704116 reads in 141.163 CPU sec, 4.493 real sec
[M::process] read 700058 sequences (100000203 bp) in 1.649 CPU sec, 1.666 real sec...
[M::mem_process_seqs] Processed 703240 reads in 88.418 CPU sec, 2.784 real sec
sorting block 81, #reads = 1092712 mem = 536881826 peak = 1166798848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 292527, 6, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (152, 214, 308)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620)
[M::mem_pestat] mean and std.dev: (238.41, 113.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 776)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 82, #reads = 1092156 mem = 536880523 peak = 1166798848
[M::process] read 699340 sequences (100000258 bp) in 2.361 CPU sec, 2.524 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 291016, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (156, 221, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645)
[M::mem_pestat] mean and std.dev: (245.34, 117.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 808)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 702790 reads in 138.390 CPU sec, 4.419 real sec
[M::mem_process_seqs] Processed 700058 reads in 88.359 CPU sec, 2.785 real sec
[M::process] read 699084 sequences (100000077 bp) in 2.320 CPU sec, 2.463 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 290849, 7, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 319)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643)
[M::mem_pestat] mean and std.dev: (246.37, 116.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 805)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (218, 332, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (276.11, 121.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1330)
[M::mem_pestat] skip orientation RR
sorting block 83, #reads = 1091608 mem = 536878952 peak = 1166798848
[M::process] read 698288 sequences (100000199 bp) in 2.431 CPU sec, 2.615 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 291278, 6, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (157, 222, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640)
[M::mem_pestat] mean and std.dev: (245.47, 115.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 801)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 374, 582)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290)
[M::mem_pestat] mean and std.dev: (319.80, 164.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1644)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 699340 reads in 135.648 CPU sec, 4.342 real sec
[M::mem_process_seqs] Processed 699084 reads in 86.638 CPU sec, 2.738 real sec
sorting block 84, #reads = 1090025 mem = 536876773 peak = 1166798848
[M::process] read 697940 sequences (100000046 bp) in 2.646 CPU sec, 2.851 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 290638, 10, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 224, 318)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638)
[M::mem_pestat] mean and std.dev: (246.19, 115.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 798)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (281, 1604, 1665)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4433)
[M::mem_pestat] mean and std.dev: (1224.00, 866.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5817)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 289725, 5, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (374, 741, 1153)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2711)
[M::mem_pestat] mean and std.dev: (816.20, 662.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3490)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 320)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644)
[M::mem_pestat] mean and std.dev: (247.63, 116.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 806)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697750 sequences (100000007 bp) in 2.139 CPU sec, 2.242 real sec...
[M::mem_process_seqs] Processed 698288 reads in 134.960 CPU sec, 4.347 real sec
[M::mem_process_seqs] Processed 697940 reads in 84.868 CPU sec, 2.703 real sec
sorting block 85, #reads = 1090946 mem = 536879039 peak = 1166798848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 290683, 3, 9)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (206, 219, 310)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518)
[M::mem_pestat] mean and std.dev: (210.64, 64.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 622)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 226, 325)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657)
[M::mem_pestat] mean and std.dev: (250.37, 119.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 823)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 697422 sequences (100000141 bp) in 2.312 CPU sec, 2.473 real sec...
[M::mem_process_seqs] Processed 697750 reads in 72.699 CPU sec, 2.400 real sec
[M::process] read 276304 sequences (39586514 bp) in 1.176 CPU sec, 1.247 real sec...
sorting block 86, #reads = 1090876 mem = 536878635 peak = 1166798848
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 290515, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 227, 327)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663)
[M::mem_pestat] mean and std.dev: (251.65, 120.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 831)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 115438, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (158, 225, 324)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656)
[M::mem_pestat] mean and std.dev: (249.96, 119.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 822)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 697422 reads in 95.893 CPU sec, 3.115 real sec
[M::mem_process_seqs] Processed 276304 reads in 41.140 CPU sec, 1.337 real sec
sorting block 87, #reads = 1091885 mem = 536881922 peak = 1166798848
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 96271212
overall: 69831491584 mem 11290.050 user 368.752 sys 387.767 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.1047_JG-T1-TDNA-1_A23MHWWLT4_1.L004\tPU:L004\tSM:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tLB:1047_JG-T1-TDNA-1_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R1.fastq.gz 0002.1047_JG-T1-TDNA-1_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 387.808 sec; CPU: 11658.804 sec
sorting block 88, #reads = 538197 mem = 265233981 peak = 1166798848
uniq header count 1
merging 89 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000a06d1
  features: 1f8bfbff fffab20b
  extended: f1bf27eb 1b407f7e
  amd bits: 2c100800 00000121
     brand: Intel(R) Xeon(R) 6975P-C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 5227734434
output reads: 96764234
bam_mem_sort: 89 calls 32.938 user 0.439 sys 33.612 real
bam_write: 89 calls 57.969 user 1.503 sys 59.884 real
execute: 1 calls 17.846 user 1.104 sys 415.187 real
merge_files: 1 calls 17.828 user 1.075 sys 26.713 real
parse_chunk: 1416 calls 45.080 user 12.886 sys 58.905 real
read_chunk: 3825 calls 37.197 user 35.210 sys 338.028 real
sort_block: 1 calls 29.138 user 34.523 sys 326.055 real
write_chunk: 2407 calls 17.524 user 0.792 sys 25.899 real
overall: 3083505664 mem 1043.193 user 59.924 sys 415.215 real
ls: cannot access '*.cram': No such file or directory