This software is licensed to bgold@natera.com by Sentieon Inc.
cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1522.268 CPU sec, 57.341 real sec
[M::process] read 675562 sequences (100000219 bp) in 1.258 CPU sec, 1.262 real sec...
[M::process] read 672786 sequences (100000154 bp) in 1.370 CPU sec, 1.369 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281049, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 376, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (386.66, 172.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673396 sequences (100000060 bp) in 1.339 CPU sec, 1.347 real sec...
[M::process] read 673752 sequences (100000099 bp) in 1.270 CPU sec, 1.261 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280193, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (280, 405, 531)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (411.01, 174.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675562 reads in 152.585 CPU sec, 4.801 real sec
[M::mem_process_seqs] Processed 672786 reads in 96.033 CPU sec, 3.052 real sec
sorting block 0, #reads = 1064315 mem = 536877814 peak = 583770112
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280171, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 401, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (406.92, 174.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674834 sequences (100000284 bp) in 1.743 CPU sec, 2.015 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280163, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 398, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (404.73, 174.96)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674830 sequences (100000064 bp) in 2.000 CPU sec, 1.999 real sec...
[M::mem_process_seqs] Processed 673396 reads in 147.253 CPU sec, 4.690 real sec
[M::mem_process_seqs] Processed 673752 reads in 98.544 CPU sec, 3.169 real sec
sorting block 1, #reads = 1063436 mem = 536880609 peak = 713961472
[M::process] read 675254 sequences (100000202 bp) in 1.781 CPU sec, 1.879 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281383, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 386, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (394.82, 174.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675138 sequences (100000132 bp) in 1.620 CPU sec, 1.622 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281274, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 387, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (395.19, 174.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1294)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280613, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (390.55, 173.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674834 reads in 203.977 CPU sec, 6.538 real sec
[M::mem_process_seqs] Processed 674830 reads in 150.656 CPU sec, 4.864 real sec
sorting block 2, #reads = 1063933 mem = 536878573 peak = 860954624
[M::mem_process_seqs] Processed 675254 reads in 163.896 CPU sec, 5.300 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280409, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
sorting block 3, #reads = 1064275 mem = 536880804 peak = 860954624
[M::mem_pestat] (25, 50, 75) percentile: (257, 384, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (393.14, 174.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672560 sequences (100000185 bp) in 2.579 CPU sec, 2.749 real sec...
[M::mem_process_seqs] Processed 675138 reads in 79.721 CPU sec, 2.616 real sec
sorting block 4, #reads = 1065657 mem = 536878876 peak = 860954624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279811, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (284, 412, 540)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (417.30, 176.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (322, 408, 408)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (150, 580)
[M::mem_pestat] mean and std.dev: (349.38, 70.01)
[M::mem_pestat] low and high boundaries for proper pairs: (64, 666)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 672560 reads in 81.171 CPU sec, 2.634 real sec
[M::process] read 673746 sequences (100000018 bp) in 2.528 CPU sec, 2.789 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 280379, 0, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (221, 221, 387)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719)
[M::mem_pestat] mean and std.dev: (311.60, 142.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 885)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 399, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (405.79, 175.87)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 675304 sequences (100000301 bp) in 2.447 CPU sec, 2.612 real sec...
[M::mem_process_seqs] Processed 673746 reads in 84.327 CPU sec, 2.714 real sec
sorting block 5, #reads = 1063545 mem = 536882338 peak = 860954624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280163, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 384, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (392.97, 175.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675304 reads in 88.114 CPU sec, 2.831 real sec
[M::process] read 674652 sequences (100000139 bp) in 2.770 CPU sec, 2.978 real sec...
sorting block 6, #reads = 1065172 mem = 536879431 peak = 860954624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279733, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 389, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (397.65, 175.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (333, 333, 350)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (299, 384)
[M::mem_pestat] mean and std.dev: (341.50, 8.50)
[M::mem_pestat] low and high boundaries for proper pairs: (282, 401)
[M::mem_pestat] skip orientation RR
[M::process] read 674384 sequences (100000183 bp) in 2.622 CPU sec, 2.730 real sec...
[M::mem_process_seqs] Processed 674652 reads in 87.867 CPU sec, 2.829 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280514, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 388, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (396.74, 174.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674382 sequences (100000093 bp) in 2.682 CPU sec, 2.826 real sec...
[M::mem_process_seqs] Processed 674384 reads in 90.864 CPU sec, 2.921 real sec
sorting block 7, #reads = 1064280 mem = 536879507 peak = 860954624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280470, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 387, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (396.27, 174.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674624 sequences (100000138 bp) in 2.675 CPU sec, 2.858 real sec...
[M::mem_process_seqs] Processed 674382 reads in 93.126 CPU sec, 2.988 real sec
[M::process] read 673066 sequences (100000129 bp) in 2.451 CPU sec, 2.608 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280008, 4, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (392.69, 172.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673522 sequences (100000282 bp) in 1.743 CPU sec, 1.756 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279553, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 399, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (405.87, 174.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1296)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674624 reads in 155.794 CPU sec, 4.920 real sec
sorting block 8, #reads = 1064347 mem = 536878655 peak = 860954624
[M::mem_process_seqs] Processed 673066 reads in 98.340 CPU sec, 3.088 real sec
[M::process] read 674712 sequences (100000182 bp) in 2.396 CPU sec, 2.558 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279457, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 392, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (399.73, 173.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 9, #reads = 1064186 mem = 536878804 peak = 860954624
[M::process] read 673846 sequences (100000230 bp) in 2.606 CPU sec, 2.751 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280953, 1, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 379, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (388.43, 171.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673522 reads in 155.466 CPU sec, 4.951 real sec
[M::mem_process_seqs] Processed 674712 reads in 97.725 CPU sec, 3.078 real sec
[M::process] read 673642 sequences (100000275 bp) in 2.572 CPU sec, 2.724 real sec...
sorting block 10, #reads = 1064018 mem = 536881089 peak = 860954624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279513, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 390, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (398.86, 173.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674334 sequences (100000048 bp) in 2.498 CPU sec, 2.771 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279706, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 390, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (398.39, 171.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673846 reads in 160.035 CPU sec, 5.084 real sec
[M::mem_process_seqs] Processed 673642 reads in 101.169 CPU sec, 3.188 real sec
[M::process] read 674334 sequences (100000206 bp) in 2.215 CPU sec, 2.327 real sec...
sorting block 11, #reads = 1064297 mem = 536880725 peak = 860954624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279722, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 383, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (392.41, 172.17)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674112 sequences (100000112 bp) in 2.325 CPU sec, 2.491 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279809, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 378, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (388.57, 169.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674334 reads in 155.969 CPU sec, 4.968 real sec
[M::mem_process_seqs] Processed 674334 reads in 96.519 CPU sec, 3.039 real sec
[M::process] read 672944 sequences (100000180 bp) in 1.933 CPU sec, 1.993 real sec...
sorting block 12, #reads = 1064419 mem = 536880016 peak = 860954624
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 279797, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 384, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (393.77, 172.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673788 sequences (100000116 bp) in 2.473 CPU sec, 2.634 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 279320, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 394, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (402.52, 172.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674112 reads in 163.864 CPU sec, 5.225 real sec
sorting block 13, #reads = 1064680 mem = 536882650 peak = 860954624
[M::mem_process_seqs] Processed 672944 reads in 100.828 CPU sec, 3.180 real sec
[M::process] read 674590 sequences (100000168 bp) in 2.256 CPU sec, 2.307 real sec...
sorting block 14, #reads = 1064678 mem = 536882572 peak = 881750016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279337, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 386, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (394.82, 171.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674546 sequences (100000109 bp) in 2.622 CPU sec, 2.756 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279917, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 378, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (389.24, 171.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673788 reads in 159.934 CPU sec, 5.106 real sec
[M::mem_process_seqs] Processed 674590 reads in 100.410 CPU sec, 3.175 real sec
[M::process] read 673714 sequences (100000233 bp) in 2.250 CPU sec, 2.317 real sec...
sorting block 15, #reads = 1063819 mem = 536878836 peak = 881750016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280139, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (388.04, 171.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674290 sequences (100000099 bp) in 2.814 CPU sec, 2.967 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279614, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 388, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (397.66, 172.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1262)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674546 reads in 152.146 CPU sec, 4.841 real sec
[M::mem_process_seqs] Processed 673714 reads in 101.527 CPU sec, 3.195 real sec
sorting block 16, #reads = 1064873 mem = 536879881 peak = 881750016
[M::process] read 674388 sequences (100000022 bp) in 2.949 CPU sec, 3.143 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280316, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 384, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (393.78, 173.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1275)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673902 sequences (100000135 bp) in 2.955 CPU sec, 3.161 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 279992, 1, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (253, 446, 559)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171)
[M::mem_pestat] mean and std.dev: (384.17, 166.63)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1477)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 387, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (396.64, 175.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674290 reads in 163.710 CPU sec, 5.227 real sec
[M::mem_process_seqs] Processed 674388 reads in 105.964 CPU sec, 3.354 real sec
sorting block 17, #reads = 1065222 mem = 536882277 peak = 881750016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280291, 4, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 390, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (399.98, 176.28)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673404 sequences (100000139 bp) in 3.251 CPU sec, 3.529 real sec...
[M::mem_process_seqs] Processed 673902 reads in 89.067 CPU sec, 2.931 real sec
sorting block 18, #reads = 1065409 mem = 536881529 peak = 881750016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279923, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 396, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (406.13, 178.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673404 reads in 89.704 CPU sec, 2.943 real sec
[M::process] read 674084 sequences (100000174 bp) in 3.400 CPU sec, 3.678 real sec...
sorting block 19, #reads = 1064647 mem = 536878834 peak = 881750016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280890, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 391, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (400.58, 175.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674084 reads in 88.568 CPU sec, 2.892 real sec
[M::process] read 674524 sequences (100000247 bp) in 3.218 CPU sec, 3.460 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280416, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 384, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (394.07, 174.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674524 reads in 85.003 CPU sec, 2.735 real sec
[M::process] read 674352 sequences (100000248 bp) in 2.633 CPU sec, 2.781 real sec...
sorting block 20, #reads = 1063489 mem = 536878999 peak = 881750016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280690, 3, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 388, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (397.51, 174.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (126, 231, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206)
[M::mem_pestat] mean and std.dev: (261.23, 155.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1566)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674352 reads in 84.411 CPU sec, 2.721 real sec
[M::process] read 673690 sequences (100000047 bp) in 2.981 CPU sec, 3.218 real sec...
sorting block 21, #reads = 1066242 mem = 536882358 peak = 881750016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280064, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 398, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (405.51, 175.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674302 sequences (100000109 bp) in 2.557 CPU sec, 2.692 real sec...
[M::mem_process_seqs] Processed 673690 reads in 86.523 CPU sec, 2.803 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280467, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 390, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (398.24, 175.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674302 reads in 86.745 CPU sec, 2.801 real sec
[M::process] read 674132 sequences (100000116 bp) in 3.002 CPU sec, 3.240 real sec...
sorting block 22, #reads = 1065628 mem = 536881054 peak = 881750016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279792, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 393, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (401.69, 176.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1290)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674132 reads in 91.209 CPU sec, 2.936 real sec
[M::process] read 673942 sequences (100000068 bp) in 2.868 CPU sec, 3.019 real sec...
sorting block 23, #reads = 1066369 mem = 536875872 peak = 881750016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280550, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (268, 396, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (403.63, 176.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (221, 225, 302)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (59, 464)
[M::mem_pestat] mean and std.dev: (240.09, 68.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 545)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 673942 reads in 87.549 CPU sec, 2.834 real sec
[M::process] read 674334 sequences (100000114 bp) in 3.233 CPU sec, 3.495 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280224, 5, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 387, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (396.28, 174.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674556 sequences (100000221 bp) in 2.554 CPU sec, 2.652 real sec...
[M::mem_process_seqs] Processed 674334 reads in 85.884 CPU sec, 2.772 real sec
sorting block 24, #reads = 1065733 mem = 536876355 peak = 881750016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280664, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 389, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (397.47, 175.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674556 reads in 87.308 CPU sec, 2.832 real sec
[M::process] read 673310 sequences (100000124 bp) in 3.361 CPU sec, 3.651 real sec...
sorting block 25, #reads = 1066721 mem = 536882497 peak = 881750016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280082, 6, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 400, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (407.42, 175.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673310 reads in 86.877 CPU sec, 2.808 real sec
[M::process] read 674156 sequences (100000136 bp) in 2.699 CPU sec, 2.849 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280265, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 392, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (400.83, 175.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674156 reads in 86.414 CPU sec, 2.802 real sec
sorting block 26, #reads = 1066321 mem = 536879363 peak = 881750016
[M::process] read 674524 sequences (100000119 bp) in 3.829 CPU sec, 4.217 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280285, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 388, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (396.99, 175.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (173, 290, 338)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 668)
[M::mem_pestat] mean and std.dev: (236.50, 75.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 833)
[M::mem_pestat] skip orientation RR
[M::process] read 674172 sequences (100000160 bp) in 2.300 CPU sec, 2.380 real sec...
[M::mem_process_seqs] Processed 674524 reads in 86.905 CPU sec, 2.781 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280906, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 389, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (398.37, 175.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674172 reads in 86.111 CPU sec, 2.765 real sec
sorting block 27, #reads = 1066363 mem = 536879329 peak = 881750016
[M::process] read 674300 sequences (100000162 bp) in 3.152 CPU sec, 3.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280517, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 388, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (397.13, 174.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674300 reads in 85.937 CPU sec, 2.771 real sec
[M::process] read 674324 sequences (100000194 bp) in 3.040 CPU sec, 3.288 real sec...
sorting block 28, #reads = 1066442 mem = 536883127 peak = 881750016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280968, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 390, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (398.95, 175.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674324 reads in 86.147 CPU sec, 2.783 real sec
[M::process] read 674116 sequences (100000212 bp) in 3.322 CPU sec, 3.561 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281217, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 389, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (398.32, 174.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672726 sequences (100000272 bp) in 2.270 CPU sec, 2.358 real sec...
[M::mem_process_seqs] Processed 674116 reads in 85.408 CPU sec, 2.734 real sec
sorting block 29, #reads = 1065788 mem = 536877479 peak = 881750016
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 279926, 0, 3)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (189, 326, 1409)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3849)
[M::mem_pestat] mean and std.dev: (558.58, 498.04)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 5069)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 402, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (409.60, 175.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 673034 sequences (100000123 bp) in 2.782 CPU sec, 2.930 real sec...
[M::mem_process_seqs] Processed 672726 reads in 85.532 CPU sec, 2.750 real sec
sorting block 30, #reads = 1066045 mem = 536881952 peak = 903122944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280093, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 399, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (406.39, 175.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1285)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674462 sequences (100000188 bp) in 2.471 CPU sec, 2.602 real sec...
[M::mem_process_seqs] Processed 673034 reads in 84.528 CPU sec, 2.713 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280834, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 383, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (394.22, 175.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (494, 543, 722)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (38, 1178)
[M::mem_pestat] mean and std.dev: (517.00, 220.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1406)
[M::mem_pestat] skip orientation RR
[M::process] read 674124 sequences (100000180 bp) in 2.484 CPU sec, 2.593 real sec...
[M::mem_process_seqs] Processed 674462 reads in 86.832 CPU sec, 2.780 real sec
sorting block 31, #reads = 1064601 mem = 536879845 peak = 903122944
[M::process] read 674150 sequences (100000227 bp) in 2.476 CPU sec, 2.595 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280891, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 386, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (396.26, 175.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674308 sequences (100000073 bp) in 1.670 CPU sec, 1.670 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280262, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 390, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (400.13, 178.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674124 reads in 153.525 CPU sec, 4.825 real sec
[M::mem_process_seqs] Processed 674150 reads in 102.174 CPU sec, 3.205 real sec
[M::process] read 674402 sequences (100000087 bp) in 2.134 CPU sec, 2.206 real sec...
sorting block 32, #reads = 1066766 mem = 536879418 peak = 903122944
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280261, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 388, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (398.34, 177.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1300)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674294 sequences (100000276 bp) in 2.816 CPU sec, 2.978 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280437, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 383, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (394.65, 175.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674308 reads in 159.277 CPU sec, 5.065 real sec
[M::mem_process_seqs] Processed 674402 reads in 106.799 CPU sec, 3.370 real sec
sorting block 33, #reads = 1064867 mem = 536882360 peak = 903122944
[M::process] read 672998 sequences (100000213 bp) in 2.683 CPU sec, 2.783 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280476, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (393.38, 173.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674224 sequences (100000032 bp) in 2.823 CPU sec, 2.956 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280311, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (274, 398, 526)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (406.64, 175.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674294 reads in 162.053 CPU sec, 5.154 real sec
sorting block 34, #reads = 1066204 mem = 536882034 peak = 920297472
[M::mem_process_seqs] Processed 672998 reads in 105.546 CPU sec, 3.329 real sec
[M::process] read 674454 sequences (100000123 bp) in 2.729 CPU sec, 2.833 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280732, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 384, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (394.91, 174.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 35, #reads = 1065142 mem = 536879276 peak = 920297472
[M::process] read 674420 sequences (100000014 bp) in 2.697 CPU sec, 2.799 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281168, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (392.76, 175.34)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674224 reads in 157.867 CPU sec, 5.030 real sec
[M::mem_process_seqs] Processed 674454 reads in 101.934 CPU sec, 3.212 real sec
sorting block 36, #reads = 1066341 mem = 536878666 peak = 920297472
[M::process] read 674446 sequences (100000081 bp) in 2.655 CPU sec, 2.741 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280950, 6, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (393.82, 175.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (310, 397, 731)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1573)
[M::mem_pestat] mean and std.dev: (446.33, 218.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1994)
[M::mem_pestat] skip orientation RR
[M::process] read 674348 sequences (100000153 bp) in 2.587 CPU sec, 2.655 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280855, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (393.14, 175.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674420 reads in 153.000 CPU sec, 4.858 real sec
[M::mem_process_seqs] Processed 674446 reads in 101.970 CPU sec, 3.210 real sec
sorting block 37, #reads = 1065088 mem = 536883823 peak = 920297472
[M::process] read 674574 sequences (100000022 bp) in 2.666 CPU sec, 2.777 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 280425, 1, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (1020, 1020, 1548)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2604)
[M::mem_pestat] mean and std.dev: (1028.50, 452.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3132)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 384, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (394.90, 175.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674550 sequences (100000075 bp) in 2.697 CPU sec, 2.770 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281261, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 387, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (397.47, 178.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674348 reads in 153.425 CPU sec, 4.870 real sec
[M::mem_process_seqs] Processed 674574 reads in 101.012 CPU sec, 3.176 real sec
sorting block 38, #reads = 1065349 mem = 536878736 peak = 920297472
[M::process] read 674276 sequences (100000091 bp) in 2.864 CPU sec, 2.964 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281114, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 386, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (397.44, 178.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 39, #reads = 1065845 mem = 536879533 peak = 920297472
[M::process] read 675824 sequences (100000058 bp) in 2.532 CPU sec, 2.571 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280990, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 389, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (400.17, 179.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1309)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674550 reads in 156.466 CPU sec, 4.961 real sec
[M::mem_process_seqs] Processed 674276 reads in 104.481 CPU sec, 3.287 real sec
[M::process] read 675280 sequences (100000203 bp) in 2.885 CPU sec, 3.005 real sec...
sorting block 40, #reads = 1064436 mem = 536879993 peak = 920297472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280885, 18, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 373, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (381.67, 167.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] analyzing insert size distribution for orientation RF...
[M::mem_pestat] (25, 50, 75) percentile: (704, 2451, 2451)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5945)
[M::mem_pestat] mean and std.dev: (1865.67, 844.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 7692)
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation RF
[M::process] read 675382 sequences (100000188 bp) in 2.445 CPU sec, 2.550 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280991, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 375, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978)
[M::mem_pestat] mean and std.dev: (382.91, 166.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675824 reads in 166.321 CPU sec, 5.272 real sec
[M::mem_process_seqs] Processed 675280 reads in 109.564 CPU sec, 3.445 real sec
sorting block 41, #reads = 1063368 mem = 536878221 peak = 920297472
[M::process] read 674890 sequences (100000026 bp) in 2.241 CPU sec, 2.339 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281300, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 376, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (383.91, 167.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674496 sequences (100000237 bp) in 2.323 CPU sec, 2.387 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281331, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 380, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (387.25, 167.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1231)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674278 sequences (100000225 bp) in 1.760 CPU sec, 1.759 real sec...
[M::mem_process_seqs] Processed 675382 reads in 160.895 CPU sec, 5.081 real sec
[M::mem_process_seqs] Processed 674890 reads in 105.751 CPU sec, 3.331 real sec
sorting block 42, #reads = 1063620 mem = 536877928 peak = 920297472
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280551, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 381, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (388.09, 167.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1228)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672852 sequences (100000197 bp) in 2.808 CPU sec, 2.907 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280885, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 389, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (395.92, 171.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674496 reads in 156.290 CPU sec, 4.937 real sec
[M::process] read 673350 sequences (100000155 bp) in 2.087 CPU sec, 2.102 real sec...
sorting block 43, #reads = 1064777 mem = 536881159 peak = 922718208
[M::mem_process_seqs] Processed 674278 reads in 104.313 CPU sec, 3.276 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279467, 3, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 407, 530)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026)
[M::mem_pestat] mean and std.dev: (411.15, 172.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 554, 554)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1174)
[M::mem_pestat] mean and std.dev: (460.62, 180.51)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1484)
[M::mem_pestat] skip orientation RR
sorting block 44, #reads = 1064404 mem = 536879909 peak = 1021558784
[M::process] read 674920 sequences (100000118 bp) in 2.958 CPU sec, 3.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280335, 2, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 397, 520)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (402.56, 171.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (183, 285, 285)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489)
[M::mem_pestat] mean and std.dev: (213.50, 58.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 591)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 672852 reads in 165.549 CPU sec, 5.292 real sec
[M::mem_process_seqs] Processed 673350 reads in 111.421 CPU sec, 3.536 real sec
[M::process] read 674852 sequences (100000286 bp) in 2.630 CPU sec, 2.682 real sec...
sorting block 45, #reads = 1063394 mem = 536879639 peak = 1021558784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280368, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 379, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (387.03, 168.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674718 sequences (100000293 bp) in 2.667 CPU sec, 2.930 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280439, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 385, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012)
[M::mem_pestat] mean and std.dev: (392.11, 171.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674920 reads in 169.267 CPU sec, 5.422 real sec
[M::mem_process_seqs] Processed 674852 reads in 113.147 CPU sec, 3.592 real sec
[M::process] read 674666 sequences (100000273 bp) in 2.588 CPU sec, 2.651 real sec...
sorting block 46, #reads = 1064690 mem = 536879426 peak = 1021558784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280355, 1, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 385, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1010)
[M::mem_pestat] mean and std.dev: (392.48, 171.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (602, 602, 602)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (602, 602)
[M::mem_pestat] mean and std.dev: (602.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (602, 602)
[M::mem_pestat] skip orientation RR
[M::process] read 674666 sequences (100000072 bp) in 2.922 CPU sec, 3.158 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280183, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 384, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (390.95, 169.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674718 reads in 171.886 CPU sec, 5.511 real sec
[M::mem_process_seqs] Processed 674666 reads in 115.308 CPU sec, 3.653 real sec
sorting block 47, #reads = 1065216 mem = 536877799 peak = 1021558784
[M::process] read 674484 sequences (100000159 bp) in 2.672 CPU sec, 2.759 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280456, 3, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 383, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (391.18, 170.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674274 sequences (100000192 bp) in 2.871 CPU sec, 3.097 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280678, 0, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 385, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (393.49, 171.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674666 reads in 175.387 CPU sec, 5.652 real sec
sorting block 48, #reads = 1064370 mem = 536882390 peak = 1021558784
[M::mem_process_seqs] Processed 674484 reads in 115.327 CPU sec, 3.668 real sec
[M::process] read 673462 sequences (100000230 bp) in 2.504 CPU sec, 2.619 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279792, 5, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 388, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (396.39, 173.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1282)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 49, #reads = 1065311 mem = 536879019 peak = 1021558784
[M::process] read 672870 sequences (100000110 bp) in 2.647 CPU sec, 2.889 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 279967, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 393, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (399.78, 171.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674274 reads in 174.962 CPU sec, 5.614 real sec
[M::mem_process_seqs] Processed 673462 reads in 114.841 CPU sec, 3.640 real sec
[M::process] read 674002 sequences (100000054 bp) in 2.412 CPU sec, 2.507 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280302, 7, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (276, 399, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (404.58, 170.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 50, #reads = 1064653 mem = 536877258 peak = 1021558784
[M::process] read 674456 sequences (100000152 bp) in 3.015 CPU sec, 3.212 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280296, 1, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 387, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (395.07, 171.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1263)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (151, 327, 472)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114)
[M::mem_pestat] mean and std.dev: (330.55, 161.33)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1435)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 672870 reads in 170.666 CPU sec, 5.469 real sec
[M::mem_process_seqs] Processed 674002 reads in 114.607 CPU sec, 3.632 real sec
[M::process] read 674226 sequences (100000059 bp) in 2.502 CPU sec, 2.660 real sec...
sorting block 51, #reads = 1063921 mem = 536880530 peak = 1021558784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280437, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 384, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (392.86, 172.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674258 sequences (100000272 bp) in 2.813 CPU sec, 2.940 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280268, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 386, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (394.56, 172.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (315, 315, 420)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (105, 630)
[M::mem_pestat] mean and std.dev: (308.50, 54.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 735)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674456 reads in 174.136 CPU sec, 5.595 real sec
[M::mem_process_seqs] Processed 674226 reads in 114.296 CPU sec, 3.619 real sec
sorting block 52, #reads = 1064044 mem = 536877272 peak = 1021558784
[M::process] read 674286 sequences (100000096 bp) in 2.745 CPU sec, 2.872 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279933, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 386, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (394.99, 173.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 53, #reads = 1065388 mem = 536880693 peak = 1021558784
[M::process] read 674388 sequences (100000030 bp) in 2.708 CPU sec, 2.841 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 279675, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 385, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024)
[M::mem_pestat] mean and std.dev: (394.14, 173.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674258 reads in 180.429 CPU sec, 5.775 real sec
[M::mem_process_seqs] Processed 674286 reads in 119.533 CPU sec, 3.786 real sec
[M::process] read 674576 sequences (100000283 bp) in 2.488 CPU sec, 2.550 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279553, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (390.60, 172.22)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 54, #reads = 1064005 mem = 536879906 peak = 1021558784
[M::process] read 674438 sequences (100000072 bp) in 3.071 CPU sec, 3.191 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280324, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 378, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (388.66, 171.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674388 reads in 176.164 CPU sec, 5.628 real sec
[M::mem_process_seqs] Processed 674576 reads in 114.167 CPU sec, 3.604 real sec
[M::process] read 674722 sequences (100000250 bp) in 2.535 CPU sec, 2.625 real sec...
sorting block 55, #reads = 1064849 mem = 536878913 peak = 1021558784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 280125, 0, 4)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (246, 738, 817)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1959)
[M::mem_pestat] mean and std.dev: (565.18, 302.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2530)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (391.78, 173.72)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674460 sequences (100000044 bp) in 2.741 CPU sec, 2.872 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280201, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 379, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (390.00, 173.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674438 reads in 166.959 CPU sec, 5.298 real sec
[M::mem_process_seqs] Processed 674722 reads in 108.688 CPU sec, 3.412 real sec
[M::process] read 674552 sequences (100000299 bp) in 2.150 CPU sec, 2.181 real sec...
sorting block 56, #reads = 1065538 mem = 536883946 peak = 1021558784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280569, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 381, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (392.38, 174.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674430 sequences (100000231 bp) in 2.504 CPU sec, 2.597 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280003, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (392.56, 174.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674460 reads in 161.345 CPU sec, 5.120 real sec
sorting block 57, #reads = 1066054 mem = 536880662 peak = 1021558784
[M::mem_process_seqs] Processed 674552 reads in 107.291 CPU sec, 3.368 real sec
[M::process] read 674546 sequences (100000152 bp) in 2.500 CPU sec, 2.549 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280321, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 383, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (394.94, 175.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1287)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 58, #reads = 1065540 mem = 536878352 peak = 1021558784
[M::process] read 674310 sequences (100000103 bp) in 2.874 CPU sec, 2.985 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281024, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 383, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (394.63, 176.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674430 reads in 160.601 CPU sec, 5.117 real sec
[M::mem_process_seqs] Processed 674546 reads in 106.466 CPU sec, 3.365 real sec
[M::process] read 674550 sequences (100000244 bp) in 2.606 CPU sec, 2.659 real sec...
sorting block 59, #reads = 1065319 mem = 536878628 peak = 1021558784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280764, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031)
[M::mem_pestat] mean and std.dev: (396.94, 177.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674866 sequences (100000053 bp) in 2.878 CPU sec, 2.974 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281270, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 384, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (397.26, 179.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674310 reads in 162.343 CPU sec, 5.162 real sec
[M::mem_process_seqs] Processed 674550 reads in 108.779 CPU sec, 3.438 real sec
[M::process] read 673842 sequences (100000135 bp) in 2.579 CPU sec, 2.649 real sec...
sorting block 60, #reads = 1065580 mem = 536879524 peak = 1021558784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280000, 9, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 381, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (389.91, 171.03)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673616 sequences (100000024 bp) in 2.994 CPU sec, 3.112 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279825, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 385, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981)
[M::mem_pestat] mean and std.dev: (392.00, 166.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1219)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674866 reads in 171.110 CPU sec, 5.451 real sec
[M::mem_process_seqs] Processed 673842 reads in 110.165 CPU sec, 3.470 real sec
sorting block 61, #reads = 1063700 mem = 536879198 peak = 1021558784
[M::process] read 673508 sequences (100000129 bp) in 2.578 CPU sec, 2.663 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 279363, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 391, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (397.69, 169.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673868 sequences (100000208 bp) in 2.912 CPU sec, 3.028 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279615, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 390, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (396.45, 168.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673616 reads in 168.496 CPU sec, 5.360 real sec
sorting block 62, #reads = 1064205 mem = 536879584 peak = 1021558784
[M::mem_process_seqs] Processed 673508 reads in 111.385 CPU sec, 3.509 real sec
[M::process] read 674104 sequences (100000158 bp) in 2.654 CPU sec, 2.729 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 279273, 1, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (204, 290, 813)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2031)
[M::mem_pestat] mean and std.dev: (426.08, 286.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2640)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (265, 385, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (393.45, 168.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 63, #reads = 1064692 mem = 536879680 peak = 1021558784
[M::process] read 674504 sequences (100000214 bp) in 2.872 CPU sec, 2.969 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279330, 7, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 382, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (390.29, 167.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673868 reads in 173.317 CPU sec, 5.520 real sec
[M::mem_process_seqs] Processed 674104 reads in 113.292 CPU sec, 3.571 real sec
[M::process] read 672570 sequences (100000171 bp) in 2.506 CPU sec, 2.574 real sec...
sorting block 64, #reads = 1065134 mem = 536878335 peak = 1021558784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280243, 0, 19)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 383, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (390.25, 169.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1244)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 394, 899)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2215)
[M::mem_pestat] mean and std.dev: (500.47, 302.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2873)
[M::mem_pestat] skip orientation RR
[M::process] read 672520 sequences (100000121 bp) in 2.796 CPU sec, 2.968 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 278860, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (281, 404, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (410.06, 172.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1269)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674504 reads in 166.920 CPU sec, 5.319 real sec
[M::mem_process_seqs] Processed 672570 reads in 110.736 CPU sec, 3.501 real sec
[M::process] read 674746 sequences (100000251 bp) in 2.641 CPU sec, 2.732 real sec...
sorting block 65, #reads = 1065310 mem = 536881084 peak = 1021558784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 278841, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (282, 404, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (410.04, 171.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674430 sequences (100000116 bp) in 2.846 CPU sec, 2.969 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279350, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 384, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (391.74, 171.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 672520 reads in 172.738 CPU sec, 5.511 real sec
[M::mem_process_seqs] Processed 674746 reads in 115.215 CPU sec, 3.641 real sec
sorting block 66, #reads = 1064120 mem = 536880212 peak = 1021558784
[M::process] read 675092 sequences (100000185 bp) in 2.259 CPU sec, 2.326 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279541, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 388, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (395.05, 171.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 67, #reads = 1064615 mem = 536877190 peak = 1021558784
[M::process] read 674968 sequences (100000156 bp) in 3.075 CPU sec, 3.182 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280347, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 380, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (387.61, 169.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674430 reads in 176.221 CPU sec, 5.619 real sec
[M::mem_process_seqs] Processed 675092 reads in 116.429 CPU sec, 3.675 real sec
[M::process] read 674926 sequences (100000232 bp) in 2.660 CPU sec, 2.724 real sec...
sorting block 68, #reads = 1064905 mem = 536880186 peak = 1021558784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280211, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 379, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (387.47, 169.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1252)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675112 sequences (100000012 bp) in 2.771 CPU sec, 2.862 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280302, 4, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 380, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (388.17, 169.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674968 reads in 173.084 CPU sec, 5.500 real sec
[M::mem_process_seqs] Processed 674926 reads in 113.253 CPU sec, 3.568 real sec
[M::process] read 674702 sequences (100000238 bp) in 2.425 CPU sec, 2.450 real sec...
sorting block 69, #reads = 1066195 mem = 536879615 peak = 1021558784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280127, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 380, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (388.35, 170.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674286 sequences (100000053 bp) in 2.461 CPU sec, 2.564 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279772, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 382, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (390.51, 170.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 672936 sequences (100000284 bp) in 2.039 CPU sec, 2.038 real sec...
[M::mem_process_seqs] Processed 675112 reads in 172.828 CPU sec, 5.479 real sec
[M::mem_process_seqs] Processed 674702 reads in 112.453 CPU sec, 3.559 real sec
sorting block 70, #reads = 1064537 mem = 536880328 peak = 1021558784
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279581, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 387, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (394.34, 171.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674080 sequences (100000129 bp) in 2.454 CPU sec, 2.544 real sec...
[M::process] read 674480 sequences (100000067 bp) in 1.820 CPU sec, 1.820 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 279158, 8, 7)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (102, 287, 870)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2406)
[M::mem_pestat] mean and std.dev: (456.21, 372.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3174)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (275, 398, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (404.39, 171.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 674286 reads in 174.729 CPU sec, 5.543 real sec
sorting block 71, #reads = 1064821 mem = 536879898 peak = 1021558784
[M::mem_process_seqs] Processed 672936 reads in 114.237 CPU sec, 3.621 real sec
sorting block 72, #reads = 1064259 mem = 536878116 peak = 1024069632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 279739, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 388, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (395.40, 171.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1264)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674622 sequences (100000287 bp) in 2.778 CPU sec, 2.895 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 279660, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 383, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (391.07, 170.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673594 sequences (100000256 bp) in 2.027 CPU sec, 2.032 real sec...
[M::mem_process_seqs] Processed 674080 reads in 176.790 CPU sec, 5.598 real sec
[M::mem_process_seqs] Processed 674480 reads in 115.030 CPU sec, 3.642 real sec
sorting block 73, #reads = 1064438 mem = 536880306 peak = 1024069632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280100, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 385, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (393.16, 172.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674152 sequences (100000208 bp) in 2.915 CPU sec, 3.017 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279312, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (269, 395, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (401.69, 172.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674480 sequences (100000199 bp) in 1.830 CPU sec, 1.839 real sec...
[M::mem_process_seqs] Processed 674622 reads in 176.356 CPU sec, 5.588 real sec
[M::mem_process_seqs] Processed 673594 reads in 113.798 CPU sec, 3.603 real sec
sorting block 74, #reads = 1064903 mem = 536878821 peak = 1024069632
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 279233, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 389, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (395.75, 171.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674470 sequences (100000035 bp) in 2.877 CPU sec, 2.979 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 279673, 3, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 383, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (391.66, 171.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674868 sequences (100000173 bp) in 1.840 CPU sec, 1.846 real sec...
[M::mem_process_seqs] Processed 674152 reads in 175.032 CPU sec, 5.543 real sec
sorting block 75, #reads = 1063934 mem = 536878466 peak = 1024069632
[M::mem_process_seqs] Processed 674480 reads in 112.831 CPU sec, 3.568 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 279865, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 382, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (391.00, 170.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674900 sequences (100000092 bp) in 2.732 CPU sec, 2.822 real sec...
[M::process] read 674770 sequences (100000080 bp) in 1.770 CPU sec, 1.773 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280158, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 376, 504)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (385.53, 169.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1257)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674470 reads in 171.665 CPU sec, 5.437 real sec
sorting block 76, #reads = 1064134 mem = 536879570 peak = 1024069632
[M::mem_process_seqs] Processed 674868 reads in 111.097 CPU sec, 3.515 real sec
sorting block 77, #reads = 1064737 mem = 536882704 peak = 1061380096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 280332, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 378, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1007)
[M::mem_pestat] mean and std.dev: (387.23, 170.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674850 sequences (100000048 bp) in 2.997 CPU sec, 3.058 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280060, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005)
[M::mem_pestat] mean and std.dev: (387.13, 169.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1255)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674900 reads in 170.570 CPU sec, 5.382 real sec
[M::process] read 674562 sequences (100000293 bp) in 2.199 CPU sec, 2.204 real sec...
[M::mem_process_seqs] Processed 674770 reads in 111.624 CPU sec, 3.516 real sec
sorting block 78, #reads = 1064223 mem = 536878331 peak = 1061380096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 280462, 1, 0)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (163, 239, 569)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1381)
[M::mem_pestat] mean and std.dev: (394.55, 348.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1788)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 377, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (387.68, 170.88)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1259)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674398 sequences (100000253 bp) in 2.874 CPU sec, 2.948 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280459, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 382, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (392.17, 173.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1281)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674850 reads in 161.939 CPU sec, 5.112 real sec
[M::process] read 674540 sequences (100000082 bp) in 2.243 CPU sec, 2.260 real sec...
[M::mem_process_seqs] Processed 674562 reads in 104.428 CPU sec, 3.277 real sec
sorting block 79, #reads = 1063729 mem = 536880311 peak = 1061380096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280480, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 382, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (392.15, 173.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675050 sequences (100000156 bp) in 3.239 CPU sec, 3.317 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280692, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (392.12, 173.68)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674398 reads in 156.508 CPU sec, 4.963 real sec
[M::mem_process_seqs] Processed 674540 reads in 102.540 CPU sec, 3.226 real sec
sorting block 80, #reads = 1063629 mem = 536879752 peak = 1061380096
[M::process] read 677332 sequences (100000203 bp) in 2.326 CPU sec, 2.361 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281261, 0, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (124, 1025, 1025)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2827)
[M::mem_pestat] mean and std.dev: (577.70, 447.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3728)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 378, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (390.36, 176.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1288)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
sorting block 81, #reads = 1064245 mem = 536878851 peak = 1061380096
[M::process] read 674796 sequences (100000015 bp) in 2.429 CPU sec, 2.496 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282432, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 368, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050)
[M::mem_pestat] mean and std.dev: (381.64, 178.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1321)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674766 sequences (100000089 bp) in 1.700 CPU sec, 1.705 real sec...
[M::mem_process_seqs] Processed 675050 reads in 161.412 CPU sec, 5.110 real sec
[M::mem_process_seqs] Processed 677332 reads in 109.561 CPU sec, 3.458 real sec
sorting block 82, #reads = 1063492 mem = 536878543 peak = 1061380096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281664, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (260, 399, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085)
[M::mem_pestat] mean and std.dev: (405.33, 181.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1360)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676188 sequences (100000096 bp) in 2.535 CPU sec, 2.601 real sec...
[M::process] read 676214 sequences (100000065 bp) in 1.620 CPU sec, 1.627 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 280520, 1, 13)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (261, 397, 532)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074)
[M::mem_pestat] mean and std.dev: (403.36, 179.16)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1345)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (273, 273, 273)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (273, 273)
[M::mem_pestat] mean and std.dev: (273.00, 0.00)
[M::mem_pestat] low and high boundaries for proper pairs: (273, 273)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674796 reads in 158.145 CPU sec, 5.005 real sec
[M::mem_process_seqs] Processed 674766 reads in 102.414 CPU sec, 3.246 real sec
sorting block 83, #reads = 1059865 mem = 536877955 peak = 1061380096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281812, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 379, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (388.13, 175.60)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676534 sequences (100000153 bp) in 2.865 CPU sec, 2.930 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281567, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 379, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (388.23, 176.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1311)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676188 reads in 158.017 CPU sec, 4.986 real sec
[M::process] read 676380 sequences (100000257 bp) in 1.947 CPU sec, 1.950 real sec...
[M::mem_process_seqs] Processed 676214 reads in 103.960 CPU sec, 3.263 real sec
sorting block 84, #reads = 1060730 mem = 536878572 peak = 1061380096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281943, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 377, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (387.09, 176.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673738 sequences (100000165 bp) in 2.457 CPU sec, 2.528 real sec...
[M::process] read 675540 sequences (100000270 bp) in 1.680 CPU sec, 1.677 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282320, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 373, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (384.44, 174.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1304)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676534 reads in 160.682 CPU sec, 5.090 real sec
sorting block 85, #reads = 1061853 mem = 536879196 peak = 1061380096
[M::mem_process_seqs] Processed 676380 reads in 105.309 CPU sec, 3.331 real sec
sorting block 86, #reads = 1063452 mem = 536879021 peak = 1061380096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281000, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 406, 536)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066)
[M::mem_pestat] mean and std.dev: (410.06, 177.67)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1331)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676594 sequences (100000040 bp) in 2.494 CPU sec, 2.576 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281990, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 384, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036)
[M::mem_pestat] mean and std.dev: (390.50, 173.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676798 sequences (100000049 bp) in 1.790 CPU sec, 1.792 real sec...
[M::mem_process_seqs] Processed 673738 reads in 157.460 CPU sec, 4.981 real sec
[M::mem_process_seqs] Processed 675540 reads in 102.285 CPU sec, 3.228 real sec
sorting block 87, #reads = 1060685 mem = 536878889 peak = 1061380096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281722, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 373, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (383.04, 173.94)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676074 sequences (100000090 bp) in 2.552 CPU sec, 2.594 real sec...
[M::process] read 675594 sequences (100000183 bp) in 1.690 CPU sec, 1.699 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282035, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 371, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (379.98, 171.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1283)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676594 reads in 159.493 CPU sec, 5.045 real sec
[M::mem_process_seqs] Processed 676798 reads in 103.597 CPU sec, 3.272 real sec
sorting block 88, #reads = 1061857 mem = 536876843 peak = 1061380096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281228, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 380, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (387.93, 173.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673934 sequences (100000114 bp) in 2.838 CPU sec, 2.885 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281130, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 380, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1034)
[M::mem_pestat] mean and std.dev: (388.50, 173.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676074 reads in 163.853 CPU sec, 5.186 real sec
[M::process] read 674570 sequences (100000160 bp) in 2.296 CPU sec, 2.309 real sec...
[M::mem_process_seqs] Processed 675594 reads in 107.838 CPU sec, 3.373 real sec
sorting block 89, #reads = 1061108 mem = 536883956 peak = 1061380096
sorting block 90, #reads = 1061325 mem = 536881053 peak = 1061380096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280481, 0, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (267, 399, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053)
[M::mem_pestat] mean and std.dev: (404.10, 175.54)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1315)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 889, 889)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2177)
[M::mem_pestat] mean and std.dev: (704.67, 435.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2821)
[M::mem_pestat] skip orientation RR
[M::process] read 675392 sequences (100000252 bp) in 2.515 CPU sec, 2.568 real sec...
[M::process] read 674302 sequences (100000125 bp) in 1.640 CPU sec, 1.645 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281116, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 392, 524)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048)
[M::mem_pestat] mean and std.dev: (399.34, 175.07)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673934 reads in 160.324 CPU sec, 5.072 real sec
[M::mem_process_seqs] Processed 674570 reads in 105.567 CPU sec, 3.337 real sec
sorting block 91, #reads = 1059822 mem = 536878676 peak = 1061380096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280890, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 381, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (389.10, 172.80)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1299)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674802 sequences (100000171 bp) in 2.737 CPU sec, 2.785 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280996, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 393, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (399.46, 174.11)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (519, 756, 999)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1959)
[M::mem_pestat] mean and std.dev: (761.18, 444.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2539)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 675392 reads in 160.320 CPU sec, 5.056 real sec
[M::process] read 674528 sequences (100000266 bp) in 2.277 CPU sec, 2.276 real sec...
[M::mem_process_seqs] Processed 674302 reads in 104.143 CPU sec, 3.269 real sec
sorting block 92, #reads = 1061111 mem = 536877730 peak = 1061380096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280877, 4, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (388.41, 169.10)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1271)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674734 sequences (100000095 bp) in 2.806 CPU sec, 2.852 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 280755, 1, 12)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 383, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (390.10, 169.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1266)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (227, 434, 615)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1391)
[M::mem_pestat] mean and std.dev: (472.42, 279.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1779)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674802 reads in 161.314 CPU sec, 5.084 real sec
[M::process] read 673470 sequences (100000082 bp) in 2.267 CPU sec, 2.277 real sec...
[M::mem_process_seqs] Processed 674528 reads in 105.989 CPU sec, 3.327 real sec
sorting block 93, #reads = 1060788 mem = 536879116 peak = 1061380096
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280340, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (389.67, 171.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674138 sequences (100000239 bp) in 2.655 CPU sec, 2.718 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 280777, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 401, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047)
[M::mem_pestat] mean and std.dev: (405.60, 173.82)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674734 reads in 159.969 CPU sec, 5.051 real sec
[M::process] read 675226 sequences (100000176 bp) in 2.258 CPU sec, 2.262 real sec...
sorting block 94, #reads = 1060695 mem = 536879799 peak = 1061380096
[M::mem_process_seqs] Processed 673470 reads in 104.267 CPU sec, 3.279 real sec
sorting block 95, #reads = 1059691 mem = 536877818 peak = 1067896832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280578, 4, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (262, 391, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039)
[M::mem_pestat] mean and std.dev: (397.51, 172.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674816 sequences (100000203 bp) in 2.671 CPU sec, 2.719 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281023, 2, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 378, 508)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (386.75, 170.23)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (159, 241, 963)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2571)
[M::mem_pestat] mean and std.dev: (458.45, 383.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3375)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674138 reads in 163.626 CPU sec, 5.154 real sec
[M::process] read 673712 sequences (100000292 bp) in 2.210 CPU sec, 2.206 real sec...
[M::mem_process_seqs] Processed 675226 reads in 106.853 CPU sec, 3.364 real sec
sorting block 96, #reads = 1060762 mem = 536880796 peak = 1067896832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 280618, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 380, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022)
[M::mem_pestat] mean and std.dev: (388.93, 171.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1278)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674666 sequences (100000172 bp) in 2.609 CPU sec, 2.655 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280916, 2, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 396, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (402.08, 174.69)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674892 sequences (100000104 bp) in 1.720 CPU sec, 1.721 real sec...
[M::mem_process_seqs] Processed 674816 reads in 156.087 CPU sec, 4.933 real sec
[M::mem_process_seqs] Processed 673712 reads in 100.778 CPU sec, 3.179 real sec
sorting block 97, #reads = 1059954 mem = 536880692 peak = 1067896832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281023, 2, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 383, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (392.54, 173.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1292)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674630 sequences (100000276 bp) in 2.842 CPU sec, 2.892 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281724, 2, 14)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 386, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (394.83, 175.57)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (200, 431, 465)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (389.08, 244.70)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1368)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 674666 reads in 153.662 CPU sec, 4.846 real sec
[M::mem_process_seqs] Processed 674892 reads in 101.090 CPU sec, 3.162 real sec
[M::process] read 674902 sequences (100000188 bp) in 2.335 CPU sec, 2.341 real sec...
sorting block 98, #reads = 1060304 mem = 536877168 peak = 1067896832
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281179, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 389, 521)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045)
[M::mem_pestat] mean and std.dev: (397.39, 176.32)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 679018 sequences (100000221 bp) in 2.832 CPU sec, 2.876 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281501, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 389, 522)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052)
[M::mem_pestat] mean and std.dev: (397.21, 177.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1317)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674630 reads in 160.161 CPU sec, 5.049 real sec
sorting block 99, #reads = 1062241 mem = 536878665 peak = 1067896832
[M::process] read 677734 sequences (100000152 bp) in 2.425 CPU sec, 2.424 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283641, 1, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (229, 351, 482)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988)
[M::mem_pestat] mean and std.dev: (362.96, 166.20)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1241)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677530 sequences (100000190 bp) in 2.406 CPU sec, 2.415 real sec...
[M::mem_process_seqs] Processed 679018 reads in 99.035 CPU sec, 3.126 real sec
sorting block 100, #reads = 1058864 mem = 536878582 peak = 1095467008
[M::mem_process_seqs] Processed 674902 reads in 230.970 CPU sec, 7.280 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282971, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 364, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (371.72, 166.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676918 sequences (100000132 bp) in 2.998 CPU sec, 3.089 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 283023, 1, 15)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 362, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (370.95, 166.47)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (195, 276, 276)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (33, 438)
[M::mem_pestat] mean and std.dev: (245.33, 54.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 519)
[M::mem_pestat] skip orientation RR
[M::mem_process_seqs] Processed 677734 reads in 192.025 CPU sec, 6.072 real sec
sorting block 101, #reads = 1058799 mem = 536877501 peak = 1095467008
[M::mem_process_seqs] Processed 677530 reads in 112.951 CPU sec, 3.549 real sec
[M::process] read 677142 sequences (100000013 bp) in 2.603 CPU sec, 2.693 real sec...
sorting block 102, #reads = 1055796 mem = 536878898 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 282524, 0, 8)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (276, 536, 1063)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2637)
[M::mem_pestat] mean and std.dev: (617.75, 380.27)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3424)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 367, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 998)
[M::mem_pestat] mean and std.dev: (375.95, 168.15)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1250)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 677084 sequences (100000050 bp) in 2.797 CPU sec, 2.845 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 282315, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 367, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (375.35, 167.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1246)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676918 reads in 165.521 CPU sec, 5.239 real sec
[M::mem_process_seqs] Processed 677142 reads in 107.763 CPU sec, 3.379 real sec
[M::process] read 675216 sequences (100000000 bp) in 2.333 CPU sec, 2.349 real sec...
sorting block 103, #reads = 1058063 mem = 536879636 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283166, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (240, 365, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996)
[M::mem_pestat] mean and std.dev: (374.16, 168.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676852 sequences (100000113 bp) in 2.817 CPU sec, 2.885 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282084, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 383, 510)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1018)
[M::mem_pestat] mean and std.dev: (390.45, 171.93)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677084 reads in 155.606 CPU sec, 4.924 real sec
[M::mem_process_seqs] Processed 675216 reads in 101.022 CPU sec, 3.172 real sec
[M::process] read 676874 sequences (100000110 bp) in 2.464 CPU sec, 2.474 real sec...
sorting block 104, #reads = 1059175 mem = 536882389 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 282759, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 367, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (376.24, 169.13)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677020 sequences (100000183 bp) in 2.745 CPU sec, 2.797 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282901, 3, 9)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 366, 494)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000)
[M::mem_pestat] mean and std.dev: (375.42, 168.99)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1253)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676852 reads in 156.822 CPU sec, 4.958 real sec
[M::mem_process_seqs] Processed 676874 reads in 103.233 CPU sec, 3.236 real sec
sorting block 105, #reads = 1059051 mem = 536878928 peak = 1095467008
[M::process] read 676438 sequences (100000081 bp) in 2.416 CPU sec, 2.425 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282725, 5, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 368, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (376.89, 169.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 106, #reads = 1061498 mem = 536877698 peak = 1095467008
[M::process] read 675324 sequences (100000065 bp) in 3.092 CPU sec, 3.177 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283049, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (245, 372, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016)
[M::mem_pestat] mean and std.dev: (382.13, 172.65)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677020 reads in 158.656 CPU sec, 5.019 real sec
[M::mem_process_seqs] Processed 676438 reads in 104.449 CPU sec, 3.282 real sec
[M::process] read 673668 sequences (100000154 bp) in 3.118 CPU sec, 3.293 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281558, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 382, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (390.57, 172.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 107, #reads = 1060963 mem = 536879109 peak = 1095467008
[M::process] read 675564 sequences (100000187 bp) in 2.732 CPU sec, 2.880 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281214, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (270, 402, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (406.16, 175.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1302)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675324 reads in 156.114 CPU sec, 4.971 real sec
[M::mem_process_seqs] Processed 673668 reads in 103.465 CPU sec, 3.262 real sec
sorting block 108, #reads = 1059164 mem = 536877009 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281875, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 382, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (390.02, 174.24)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675334 sequences (100000121 bp) in 3.608 CPU sec, 3.830 real sec...
[M::mem_process_seqs] Processed 675564 reads in 86.854 CPU sec, 2.850 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281947, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 384, 515)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1035)
[M::mem_pestat] mean and std.dev: (392.90, 175.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 109, #reads = 1059416 mem = 536881286 peak = 1095467008
[M::mem_process_seqs] Processed 675334 reads in 85.323 CPU sec, 2.788 real sec
[M::process] read 674910 sequences (100000245 bp) in 3.547 CPU sec, 3.806 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281964, 2, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 387, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040)
[M::mem_pestat] mean and std.dev: (395.77, 176.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1301)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674816 sequences (100000223 bp) in 2.563 CPU sec, 2.625 real sec...
[M::mem_process_seqs] Processed 674910 reads in 87.777 CPU sec, 2.804 real sec
sorting block 110, #reads = 1061174 mem = 536878422 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281610, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (257, 386, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037)
[M::mem_pestat] mean and std.dev: (394.17, 174.39)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1297)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674664 sequences (100000168 bp) in 2.727 CPU sec, 2.915 real sec...
[M::mem_process_seqs] Processed 674816 reads in 86.947 CPU sec, 2.789 real sec
sorting block 111, #reads = 1061072 mem = 536877933 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281799, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 387, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1038)
[M::mem_pestat] mean and std.dev: (395.36, 175.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1298)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 673862 sequences (100000128 bp) in 2.587 CPU sec, 2.760 real sec...
[M::mem_process_seqs] Processed 674664 reads in 85.645 CPU sec, 2.750 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281290, 1, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (266, 398, 529)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055)
[M::mem_pestat] mean and std.dev: (404.96, 177.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1318)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 673862 reads in 84.627 CPU sec, 2.717 real sec
[M::process] read 673462 sequences (100000114 bp) in 2.663 CPU sec, 2.855 real sec...
sorting block 112, #reads = 1061941 mem = 536877501 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 280937, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (271, 404, 535)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1063)
[M::mem_pestat] mean and std.dev: (410.15, 178.46)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1327)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674930 sequences (100000118 bp) in 2.456 CPU sec, 2.627 real sec...
[M::mem_process_seqs] Processed 673462 reads in 86.147 CPU sec, 2.770 real sec
sorting block 113, #reads = 1060348 mem = 536879294 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281668, 0, 6)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (322, 415, 1051)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2509)
[M::mem_pestat] mean and std.dev: (568.90, 320.89)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 3238)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044)
[M::mem_pestat] mean and std.dev: (394.52, 176.55)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 674880 sequences (100000093 bp) in 2.538 CPU sec, 2.711 real sec...
[M::mem_process_seqs] Processed 674930 reads in 80.729 CPU sec, 2.596 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281290, 3, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (255, 385, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041)
[M::mem_pestat] mean and std.dev: (394.24, 176.35)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1303)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674674 sequences (100000299 bp) in 2.439 CPU sec, 2.616 real sec...
[M::mem_process_seqs] Processed 674880 reads in 82.227 CPU sec, 2.644 real sec
sorting block 114, #reads = 1061688 mem = 536878078 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 280807, 3, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 390, 525)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059)
[M::mem_pestat] mean and std.dev: (400.16, 179.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1326)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674598 sequences (100000223 bp) in 2.368 CPU sec, 2.520 real sec...
[M::mem_process_seqs] Processed 674674 reads in 81.790 CPU sec, 2.624 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281107, 5, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (258, 392, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1068)
[M::mem_pestat] mean and std.dev: (401.88, 181.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1338)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674806 sequences (100000113 bp) in 2.400 CPU sec, 2.571 real sec...
[M::mem_process_seqs] Processed 674598 reads in 81.609 CPU sec, 2.617 real sec
sorting block 115, #reads = 1060712 mem = 536879079 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281433, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 389, 527)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069)
[M::mem_pestat] mean and std.dev: (400.45, 182.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1340)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 674938 sequences (100000258 bp) in 2.282 CPU sec, 2.425 real sec...
[M::mem_process_seqs] Processed 674806 reads in 81.612 CPU sec, 2.609 real sec
sorting block 116, #reads = 1060148 mem = 536884014 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281577, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 389, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072)
[M::mem_pestat] mean and std.dev: (400.93, 182.75)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1344)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676132 sequences (100000230 bp) in 2.399 CPU sec, 2.568 real sec...
[M::mem_process_seqs] Processed 674938 reads in 82.827 CPU sec, 2.659 real sec
[M::process] read 676884 sequences (100000186 bp) in 2.344 CPU sec, 2.483 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281976, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 377, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (388.27, 177.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1307)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676152 sequences (100000123 bp) in 1.340 CPU sec, 1.344 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282389, 1, 10)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 368, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001)
[M::mem_pestat] mean and std.dev: (376.57, 168.59)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (209, 315, 315)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527)
[M::mem_pestat] mean and std.dev: (273.10, 56.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 633)
[M::mem_pestat] skip orientation RR
[M::process] read 675952 sequences (100000060 bp) in 1.270 CPU sec, 1.262 real sec...
[M::mem_process_seqs] Processed 676132 reads in 158.324 CPU sec, 4.981 real sec
sorting block 117, #reads = 1058419 mem = 536878892 peak = 1095467008
[M::process] read 675488 sequences (100000125 bp) in 1.670 CPU sec, 1.680 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281631, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019)
[M::mem_pestat] mean and std.dev: (384.52, 171.42)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676152 reads in 83.291 CPU sec, 2.813 real sec
sorting block 118, #reads = 1055294 mem = 536884251 peak = 1095467008
[M::mem_process_seqs] Processed 676884 reads in 194.663 CPU sec, 6.344 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 280786, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 376, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (384.27, 171.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675440 sequences (100000164 bp) in 2.445 CPU sec, 2.617 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 281568, 3, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 380, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (386.57, 170.58)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1272)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675952 reads in 157.977 CPU sec, 5.000 real sec
[M::process] read 674642 sequences (100000271 bp) in 1.649 CPU sec, 1.679 real sec...
sorting block 119, #reads = 1062182 mem = 536885861 peak = 1095467008
[M::mem_process_seqs] Processed 675488 reads in 92.493 CPU sec, 2.910 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281017, 0, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 383, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028)
[M::mem_pestat] mean and std.dev: (390.36, 172.71)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 120, #reads = 1062874 mem = 536878617 peak = 1095467008
[M::process] read 673400 sequences (100000193 bp) in 2.523 CPU sec, 2.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280864, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 391, 517)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (396.36, 172.00)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1279)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676386 sequences (100000299 bp) in 1.509 CPU sec, 1.515 real sec...
[M::mem_process_seqs] Processed 675440 reads in 139.885 CPU sec, 4.420 real sec
[M::mem_process_seqs] Processed 674642 reads in 89.810 CPU sec, 2.829 real sec
sorting block 121, #reads = 1062069 mem = 536877588 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281241, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (272, 400, 523)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025)
[M::mem_pestat] mean and std.dev: (403.34, 170.86)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1276)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676618 sequences (100000234 bp) in 2.475 CPU sec, 2.601 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281809, 0, 5)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (573, 573, 1161)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2337)
[M::mem_pestat] mean and std.dev: (677.50, 347.92)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 2925)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 374, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014)
[M::mem_pestat] mean and std.dev: (381.32, 169.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1270)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 673400 reads in 136.733 CPU sec, 4.333 real sec
[M::mem_process_seqs] Processed 676386 reads in 89.288 CPU sec, 2.804 real sec
[M::process] read 676816 sequences (100000187 bp) in 2.015 CPU sec, 2.070 real sec...
sorting block 122, #reads = 1061188 mem = 536878165 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 282137, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 367, 491)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 985)
[M::mem_pestat] mean and std.dev: (375.50, 166.61)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1232)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676314 sequences (100000163 bp) in 2.021 CPU sec, 2.305 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282384, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 364, 488)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (372.32, 164.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676618 reads in 135.770 CPU sec, 4.314 real sec
[M::mem_process_seqs] Processed 676816 reads in 88.676 CPU sec, 2.789 real sec
[M::process] read 676078 sequences (100000171 bp) in 1.968 CPU sec, 2.009 real sec...
sorting block 123, #reads = 1063286 mem = 536879339 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281501, 1, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (244, 366, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (375.17, 166.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1240)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675978 sequences (100000115 bp) in 2.279 CPU sec, 2.396 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281559, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 372, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997)
[M::mem_pestat] mean and std.dev: (380.16, 168.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1247)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676314 reads in 140.144 CPU sec, 4.468 real sec
sorting block 124, #reads = 1062339 mem = 536877999 peak = 1095467008
[M::mem_process_seqs] Processed 676078 reads in 89.997 CPU sec, 2.837 real sec
[M::process] read 675864 sequences (100000023 bp) in 2.325 CPU sec, 2.401 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281335, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 373, 500)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004)
[M::mem_pestat] mean and std.dev: (381.68, 169.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1256)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 125, #reads = 1061992 mem = 536879885 peak = 1095467008
[M::process] read 674328 sequences (100000245 bp) in 2.465 CPU sec, 2.553 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281650, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 377, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1017)
[M::mem_pestat] mean and std.dev: (384.77, 170.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1273)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675978 reads in 142.104 CPU sec, 4.508 real sec
[M::mem_process_seqs] Processed 675864 reads in 91.633 CPU sec, 2.884 real sec
[M::process] read 674910 sequences (100000022 bp) in 2.229 CPU sec, 2.347 real sec...
sorting block 126, #reads = 1063899 mem = 536883552 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281063, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (264, 393, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (397.75, 171.30)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1284)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675826 sequences (100000039 bp) in 2.536 CPU sec, 2.719 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 282046, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (259, 386, 511)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (391.79, 170.21)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1267)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 674328 reads in 138.888 CPU sec, 4.421 real sec
[M::mem_process_seqs] Processed 674910 reads in 89.425 CPU sec, 2.820 real sec
sorting block 127, #reads = 1061851 mem = 536878294 peak = 1095467008
[M::process] read 675914 sequences (100000237 bp) in 2.420 CPU sec, 2.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281485, 6, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 372, 499)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 999)
[M::mem_pestat] mean and std.dev: (380.92, 168.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1249)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675610 sequences (100000223 bp) in 2.304 CPU sec, 2.454 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281886, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 374, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1008)
[M::mem_pestat] mean and std.dev: (382.15, 168.81)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1261)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675826 reads in 140.010 CPU sec, 4.469 real sec
[M::mem_process_seqs] Processed 675914 reads in 90.281 CPU sec, 2.848 real sec
sorting block 128, #reads = 1062636 mem = 536880139 peak = 1095467008
[M::process] read 675306 sequences (100000123 bp) in 2.303 CPU sec, 2.435 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 281141, 6, 16)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (155, 459, 459)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067)
[M::mem_pestat] mean and std.dev: (416.79, 321.53)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1703)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 375, 502)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006)
[M::mem_pestat] mean and std.dev: (383.14, 168.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1258)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 367, 537)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127)
[M::mem_pestat] mean and std.dev: (355.53, 140.01)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1422)
[M::mem_pestat] skip orientation FF
[M::mem_pestat] skip orientation RR
sorting block 129, #reads = 1062946 mem = 536876384 peak = 1095467008
[M::process] read 675358 sequences (100000089 bp) in 2.061 CPU sec, 2.135 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 281925, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 381, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021)
[M::mem_pestat] mean and std.dev: (387.81, 170.52)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675610 reads in 142.337 CPU sec, 4.530 real sec
[M::mem_process_seqs] Processed 675306 reads in 91.962 CPU sec, 2.900 real sec
[M::process] read 675352 sequences (100000297 bp) in 2.301 CPU sec, 2.428 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 280550, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 383, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033)
[M::mem_pestat] mean and std.dev: (390.03, 172.45)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1293)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 130, #reads = 1062093 mem = 536883831 peak = 1095467008
[M::process] read 675340 sequences (100000242 bp) in 2.329 CPU sec, 2.452 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281538, 2, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (252, 380, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023)
[M::mem_pestat] mean and std.dev: (387.39, 170.85)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1280)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675358 reads in 143.759 CPU sec, 4.577 real sec
[M::mem_process_seqs] Processed 675352 reads in 93.244 CPU sec, 2.938 real sec
[M::process] read 675596 sequences (100000274 bp) in 2.280 CPU sec, 2.407 real sec...
sorting block 131, #reads = 1061169 mem = 536880328 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281958, 1, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 382, 516)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (390.83, 173.95)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1305)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675228 sequences (100000213 bp) in 2.129 CPU sec, 2.211 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281480, 3, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 383, 514)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1042)
[M::mem_pestat] mean and std.dev: (390.50, 175.02)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1306)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675340 reads in 143.651 CPU sec, 4.565 real sec
[M::mem_process_seqs] Processed 675596 reads in 93.939 CPU sec, 2.959 real sec
[M::process] read 675534 sequences (100000136 bp) in 1.814 CPU sec, 1.865 real sec...
sorting block 132, #reads = 1060611 mem = 536880077 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281228, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (254, 385, 518)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1046)
[M::mem_pestat] mean and std.dev: (392.90, 174.62)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1310)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675854 sequences (100000128 bp) in 2.516 CPU sec, 2.698 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281641, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 384, 519)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051)
[M::mem_pestat] mean and std.dev: (392.76, 176.14)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1317)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675228 reads in 145.609 CPU sec, 4.636 real sec
sorting block 133, #reads = 1060552 mem = 536879329 peak = 1095467008
[M::mem_process_seqs] Processed 675534 reads in 93.861 CPU sec, 2.961 real sec
[M::process] read 676136 sequences (100000276 bp) in 1.905 CPU sec, 1.951 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281510, 2, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 377, 513)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1043)
[M::mem_pestat] mean and std.dev: (388.67, 176.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1308)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 134, #reads = 1060530 mem = 536877473 peak = 1095467008
[M::process] read 677258 sequences (100000021 bp) in 2.405 CPU sec, 2.545 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281332, 0, 8)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 369, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032)
[M::mem_pestat] mean and std.dev: (383.52, 176.48)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1295)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675854 reads in 148.733 CPU sec, 4.722 real sec
[M::process] read 678860 sequences (100000273 bp) in 1.724 CPU sec, 1.737 real sec...
[M::mem_process_seqs] Processed 676136 reads in 98.747 CPU sec, 3.111 real sec
sorting block 135, #reads = 1059408 mem = 536880104 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281939, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 364, 497)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1015)
[M::mem_pestat] mean and std.dev: (376.07, 172.79)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1274)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678024 sequences (100000249 bp) in 2.369 CPU sec, 2.461 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283442, 9, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (233, 360, 483)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (364.87, 161.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678616 sequences (100000182 bp) in 1.700 CPU sec, 1.697 real sec...
[M::mem_process_seqs] Processed 677258 reads in 159.209 CPU sec, 5.058 real sec
[M::mem_process_seqs] Processed 678860 reads in 103.033 CPU sec, 3.265 real sec
sorting block 136, #reads = 1053503 mem = 536879410 peak = 1095467008
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 283237, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (237, 364, 486)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984)
[M::mem_pestat] mean and std.dev: (367.65, 161.19)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1233)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678368 sequences (100000040 bp) in 2.624 CPU sec, 2.750 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 283143, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 354, 477)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971)
[M::mem_pestat] mean and std.dev: (360.08, 160.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1218)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675544 sequences (100000261 bp) in 1.680 CPU sec, 1.682 real sec...
[M::mem_process_seqs] Processed 678024 reads in 155.587 CPU sec, 4.930 real sec
sorting block 137, #reads = 1055387 mem = 536879101 peak = 1095467008
[M::mem_process_seqs] Processed 678616 reads in 100.091 CPU sec, 3.162 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 283605, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (232, 353, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964)
[M::mem_pestat] mean and std.dev: (360.54, 160.25)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1208)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 138, #reads = 1055593 mem = 536880014 peak = 1095467008
[M::process] read 677672 sequences (100000146 bp) in 2.630 CPU sec, 2.763 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 282034, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (200, 345, 345)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635)
[M::mem_pestat] mean and std.dev: (289.04, 129.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 807)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (256, 386, 507)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009)
[M::mem_pestat] mean and std.dev: (387.03, 165.36)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1260)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 678368 reads in 148.318 CPU sec, 4.692 real sec
[M::process] read 677836 sequences (100000095 bp) in 1.750 CPU sec, 1.748 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282415, 2, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 365, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (370.26, 163.90)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678576 sequences (100000143 bp) in 2.077 CPU sec, 2.086 real sec...
[M::mem_process_seqs] Processed 677672 reads in 81.612 CPU sec, 2.577 real sec
sorting block 139, #reads = 1057370 mem = 536879748 peak = 1095467008
[M::mem_process_seqs] Processed 675544 reads in 199.934 CPU sec, 6.325 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 282412, 1, 6)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (236, 363, 489)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 995)
[M::mem_pestat] mean and std.dev: (369.93, 165.43)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1248)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 678908 sequences (100000168 bp) in 2.514 CPU sec, 2.596 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282666, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (230, 353, 479)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 977)
[M::mem_pestat] mean and std.dev: (362.12, 162.64)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1226)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677836 reads in 163.188 CPU sec, 5.171 real sec
sorting block 140, #reads = 1056452 mem = 536878805 peak = 1095467008
[M::mem_process_seqs] Processed 678576 reads in 96.658 CPU sec, 3.045 real sec
[M::process] read 677852 sequences (100000023 bp) in 2.124 CPU sec, 2.204 real sec...
sorting block 141, #reads = 1060287 mem = 536880011 peak = 1146904576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 283305, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (228, 346, 468)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 948)
[M::mem_pestat] mean and std.dev: (354.95, 158.08)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1188)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677466 sequences (100000074 bp) in 2.117 CPU sec, 2.213 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282805, 1, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (235, 354, 476)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958)
[M::mem_pestat] mean and std.dev: (362.01, 159.66)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1199)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675760 sequences (100000118 bp) in 1.680 CPU sec, 1.675 real sec...
[M::mem_process_seqs] Processed 678908 reads in 149.800 CPU sec, 4.757 real sec
[M::mem_process_seqs] Processed 677852 reads in 95.162 CPU sec, 3.005 real sec
sorting block 142, #reads = 1058934 mem = 536878635 peak = 1146904576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282671, 0, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (238, 359, 481)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967)
[M::mem_pestat] mean and std.dev: (366.00, 160.97)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1210)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 676126 sequences (100000058 bp) in 2.323 CPU sec, 2.397 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 282247, 2, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (251, 375, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983)
[M::mem_pestat] mean and std.dev: (379.34, 163.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1227)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 677024 sequences (100000109 bp) in 1.780 CPU sec, 1.780 real sec...
[M::mem_process_seqs] Processed 677466 reads in 145.124 CPU sec, 4.592 real sec
[M::mem_process_seqs] Processed 675760 reads in 92.704 CPU sec, 2.930 real sec
sorting block 143, #reads = 1060657 mem = 536875496 peak = 1146904576
[M::process] read 676954 sequences (100000150 bp) in 2.637 CPU sec, 2.757 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 282200, 3, 3)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (242, 365, 490)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986)
[M::mem_pestat] mean and std.dev: (371.93, 163.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 677024 reads in 80.437 CPU sec, 2.520 real sec
[M::process] read 675958 sequences (100000005 bp) in 1.782 CPU sec, 1.792 real sec...
sorting block 144, #reads = 1060324 mem = 536878706 peak = 1146904576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 281698, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 371, 492)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980)
[M::mem_pestat] mean and std.dev: (376.22, 162.40)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1224)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 281775, 0, 0)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (241, 362, 487)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979)
[M::mem_pestat] mean and std.dev: (370.29, 162.91)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1225)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676126 reads in 225.422 CPU sec, 7.135 real sec
[M::process] read 676366 sequences (100000269 bp) in 1.849 CPU sec, 1.867 real sec...
[M::mem_process_seqs] Processed 676954 reads in 108.958 CPU sec, 3.461 real sec
sorting block 145, #reads = 1058939 mem = 536880103 peak = 1146904576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281627, 0, 11)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 373, 496)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990)
[M::mem_pestat] mean and std.dev: (378.83, 164.26)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1237)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation RR...
[M::mem_pestat] (25, 50, 75) percentile: (263, 263, 528)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058)
[M::mem_pestat] mean and std.dev: (388.91, 205.50)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1323)
[M::mem_pestat] skip orientation RR
[M::process] read 676184 sequences (100000155 bp) in 2.519 CPU sec, 2.628 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 281639, 0, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 370, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993)
[M::mem_pestat] mean and std.dev: (376.65, 165.37)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1242)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675958 reads in 147.070 CPU sec, 4.658 real sec
[M::process] read 675870 sequences (100000047 bp) in 1.710 CPU sec, 1.707 real sec...
sorting block 146, #reads = 1059843 mem = 536880462 peak = 1146904576
[M::mem_process_seqs] Processed 676366 reads in 93.406 CPU sec, 2.949 real sec
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281616, 1, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (246, 369, 493)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987)
[M::mem_pestat] mean and std.dev: (376.10, 165.12)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1234)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
sorting block 147, #reads = 1060525 mem = 536877804 peak = 1146904576
[M::process] read 676018 sequences (100000245 bp) in 2.484 CPU sec, 2.589 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 281777, 2, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 374, 501)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003)
[M::mem_pestat] mean and std.dev: (381.76, 167.77)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1254)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 676184 reads in 144.507 CPU sec, 4.577 real sec
[M::process] read 675684 sequences (100000026 bp) in 1.739 CPU sec, 1.743 real sec...
[M::mem_process_seqs] Processed 675870 reads in 92.021 CPU sec, 2.905 real sec
sorting block 148, #reads = 1061397 mem = 536881004 peak = 1146904576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 281592, 0, 7)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (247, 370, 495)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 991)
[M::mem_pestat] mean and std.dev: (377.85, 165.73)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1239)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675796 sequences (100000155 bp) in 2.615 CPU sec, 2.755 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 280981, 2, 2)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (175, 246, 297)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541)
[M::mem_pestat] mean and std.dev: (252.82, 90.74)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 663)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 374, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011)
[M::mem_pestat] mean and std.dev: (382.52, 168.83)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1265)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::mem_process_seqs] Processed 676018 reads in 146.147 CPU sec, 4.628 real sec
[M::process] read 675728 sequences (100000235 bp) in 1.856 CPU sec, 1.862 real sec...
[M::mem_process_seqs] Processed 675684 reads in 94.816 CPU sec, 2.986 real sec
sorting block 149, #reads = 1061260 mem = 536880355 peak = 1146904576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 281638, 0, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (248, 374, 503)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1013)
[M::mem_pestat] mean and std.dev: (382.59, 169.06)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1268)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::process] read 675532 sequences (100000212 bp) in 2.663 CPU sec, 2.783 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 281204, 2, 5)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (249, 376, 506)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020)
[M::mem_pestat] mean and std.dev: (384.67, 170.98)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1277)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675796 reads in 145.361 CPU sec, 4.607 real sec
[M::process] read 675174 sequences (100000281 bp) in 1.843 CPU sec, 1.861 real sec...
[M::mem_process_seqs] Processed 675728 reads in 93.036 CPU sec, 2.926 real sec
sorting block 150, #reads = 1062288 mem = 536879255 peak = 1146904576
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 281458, 0, 1)
[M::mem_pestat] analyzing insert size distribution for orientation FF...
[M::mem_pestat] (25, 50, 75) percentile: (230, 422, 425)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815)
[M::mem_pestat] mean and std.dev: (401.80, 164.49)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1060)
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (250, 378, 509)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027)
[M::mem_pestat] mean and std.dev: (386.73, 172.09)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1286)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_pestat] skip orientation FF
[M::process] read 520640 sequences (76965751 bp) in 2.043 CPU sec, 2.119 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 280941, 1, 4)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (253, 381, 512)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1030)
[M::mem_pestat] mean and std.dev: (389.92, 173.38)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1289)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 675532 reads in 142.593 CPU sec, 4.580 real sec
sorting block 151, #reads = 1061246 mem = 536878203 peak = 1146904576
[M::mem_process_seqs] Processed 675174 reads in 90.375 CPU sec, 2.917 real sec
sorting block 152, #reads = 1061686 mem = 536877713 peak = 1149923328
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 216808, 0, 2)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (243, 371, 505)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029)
[M::mem_pestat] mean and std.dev: (382.35, 174.31)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1291)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 520640 reads in 62.067 CPU sec, 2.060 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 162514570
overall: 69745115136 mem 22010.985 user 491.985 sys 740.683 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:A23MHWWLT4.FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1.L008\tPU:L008\tSM:FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1\tLB:FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R1.fastq.gz FFPE_HD789_01_p2_gDNA_01_A23MHWWLT4_1_R2.fastq.gz
[main] Real time: 740.726 sec; CPU: 22502.980 sec
sorting block 153, #reads = 646433 mem = 327272022 peak = 1149923328
uniq header count 1
merging 154 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 9127097972
output reads: 163252563
bam_mem_sort: 154 calls 86.768 user 3.118 sys 90.401 real
bam_write: 154 calls 61.028 user 3.144 sys 69.865 real
execute: 1 calls 41.057 user 1.942 sys 807.711 real
merge_files: 1 calls 41.032 user 1.905 sys 66.048 real
parse_chunk: 2458 calls 126.315 user 31.197 sys 161.461 real
read_chunk: 6631 calls 65.987 user 50.190 sys 703.675 real
sort_block: 1 calls 51.380 user 49.711 sys 678.221 real
write_chunk: 4171 calls 40.674 user 1.538 sys 64.997 real
overall: 3153465344 mem 2591.581 user 107.217 sys 807.732 real
ls: cannot access '*.cram': No such file or directory