This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_tissue.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2079.817 CPU sec, 79.756 real sec [M::process] read 125792 sequences (17808231 bp) in 0.167 CPU sec, 0.173 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 47271, 0, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 224, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (246.05, 119.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125792 reads in 11.755 CPU sec, 0.390 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125792 overall: 68183666688 mem 1822.776 user 276.291 sys 87.651 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_tissue.L003\tPU:L003\tSM:Sig_18_tissue\tLB:Sig_18_tissue\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_tissue_R1.fastq.gz Sig_18_tissue_R2.fastq.gz [main] Real time: 87.682 sec; CPU: 2099.070 sec sorting block 0, #reads = 126017 mem = 59991631 peak = 163577856 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7116343 output reads: 126017 bam_mem_sort: 1 calls 0.028 user 0.000 sys 0.032 real bam_write: 1 calls 0.042 user 0.002 sys 0.040 real execute: 1 calls 0.058 user 0.136 sys 88.041 real merge_files: 1 calls 0.047 user 0.104 sys 0.265 real parse_chunk: 2 calls 0.052 user 0.015 sys 0.077 real read_chunk: 8 calls 0.048 user 0.030 sys 7.446 real sort_block: 1 calls 0.106 user 0.054 sys 7.434 real write_chunk: 4 calls 0.010 user 0.018 sys 0.035 real overall: 471371776 mem 1.257 user 0.306 sys 88.059 real ls: cannot access '*.cram': No such file or directory