cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_BL.bam --sam2bam -
This software is licensed to bgold@natera.com by Sentieon Inc.
This software is licensed to bgold@natera.com by Sentieon Inc.
version: sentieon-genomics-202308.03
[M::bwa_idx_load_from_disk] read 3171 ALT contigs
[M::main_mem] bwa index loaded in 1323.785 CPU sec, 46.697 real sec
[M::process] read 124548 sequences (17983515 bp) in 0.177 CPU sec, 0.180 real sec...
[M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 44272, 0, 1)
[M::mem_pestat] skip orientation FF as there are not enough pairs
[M::mem_pestat] analyzing insert size distribution for orientation FR...
[M::mem_pestat] (25, 50, 75) percentile: (185, 282, 404)
[M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842)
[M::mem_pestat] mean and std.dev: (306.53, 153.44)
[M::mem_pestat] low and high boundaries for proper pairs: (1, 1061)
[M::mem_pestat] skip orientation RF as there are not enough pairs
[M::mem_pestat] skip orientation RR as there are not enough pairs
[M::mem_process_seqs] Processed 124548 reads in 9.276 CPU sec, 0.323 real sec
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31
algo: bwa-mem
license: sentieon:klib=31
reads: 124548
overall: 68521943040 mem 1242.502 user 95.737 sys 52.211 real
[main] Version: 0.7.17-r1188
[main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_BL.L003\tPU:L003\tSM:HCC1395_BL\tLB:HCC1395_BL\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_BL_R1.fastq.gz HCC1395_BL_R2.fastq.gz
[main] Real time: 52.251 sec; CPU: 1338.242 sec
sorting block 0, #reads = 124708 mem = 59256603 peak = 166866944
uniq header count 1
merging 1 blocks
sw version: sentieon-genomics-202308.03
os version: Linux 6.12.88
cpu vendor: GenuineIntel
 signature: 000c06f2
  features: bfebfbff 7ffefbff
  extended: f3bfb7ef fb417ffe
  amd bits: 2c100800 00000121
     brand: INTEL(R) XEON(R) PLATINUM 8559C
threads: 31 max 192
algo: util-sort
license: sentieon:util=1
output file size: 7324881
output reads: 124708
bam_mem_sort: 1 calls 0.021 user 0.000 sys 0.027 real
bam_write: 1 calls 0.109 user 0.006 sys 0.112 real
execute: 1 calls 0.078 user 0.112 sys 52.680 real
merge_files: 1 calls 0.056 user 0.092 sys 0.273 real
parse_chunk: 2 calls 0.038 user 0.026 sys 0.077 real
read_chunk: 7 calls 0.042 user 0.041 sys 5.139 real
sort_block: 1 calls 0.152 user 0.065 sys 5.184 real
write_chunk: 3 calls 0.013 user 0.015 sys 0.049 real
overall: 479641600 mem 1.240 user 0.375 sys 52.701 real
ls: cannot access '*.cram': No such file or directory