Detecting adapter sequence for read1...
>Illumina TruSeq Adapter Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA

Detecting adapter sequence for read2...
>Illumina TruSeq Adapter Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT

Read1 before filtering:
total reads: 62043
total bases: 9306450
Q20 bases: 9147412(98.2911%)
Q30 bases: 8878086(95.3971%)
Q40 bases: 8878086(95.3971%)

Read2 before filtering:
total reads: 62043
total bases: 9306450
Q20 bases: 9091938(97.695%)
Q30 bases: 8808243(94.6466%)
Q40 bases: 8808243(94.6466%)

Read1 after filtering:
total reads: 62042
total bases: 8778838
Q20 bases: 8677991(98.8512%)
Q30 bases: 8447491(96.2256%)
Q40 bases: 8447491(96.2256%)

Read2 after filtering:
total reads: 62042
total bases: 8779560
Q20 bases: 8620355(98.1866%)
Q30 bases: 8388858(95.5499%)
Q40 bases: 8388858(95.5499%)

Filtering result:
reads passed filter: 124084
reads failed due to low quality: 2
reads failed due to too many N: 0
reads failed due to too short: 0
reads with adapter trimmed: 29144
bases trimmed due to adapters: 1054236

Duplication rate: 0.752704%

Insert size peak (evaluated by paired-end reads): 179

JSON report: Sig_18_tissue.fastp.json
HTML report: Sig_18_tissue.fastp.html

fastp --in1 Sig_18_tissue_S3_L008_R1_001.fastq.gz --in2 Sig_18_tissue_S3_L008_R2_001.fastq.gz --out1 Sig_18_tissue/Sig_18_tissue_R1.fastq.gz --out2 Sig_18_tissue/Sig_18_tissue_R2.fastq.gz --detect_adapter_for_pe --length_required 15 --json Sig_18_tissue.fastp.json --html Sig_18_tissue.fastp.html --thread 4 
fastp v1.0.1, time used: 1 seconds