cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_tumor.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2133.791 CPU sec, 78.060 real sec [M::process] read 124478 sequences (17924843 bp) in 0.168 CPU sec, 0.174 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 43537, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 274, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (298.63, 150.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124478 reads in 25.368 CPU sec, 0.838 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124478 overall: 68331569152 mem 1840.259 user 325.168 sys 85.167 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_tumor.L001\tPU:L001\tSM:HCC1395_tumor\tLB:HCC1395_tumor\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_tumor_R1.fastq.gz HCC1395_tumor_R2.fastq.gz [main] Real time: 85.237 sec; CPU: 2165.429 sec sorting block 0, #reads = 124658 mem = 59579630 peak = 163651584 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7258879 output reads: 124658 bam_mem_sort: 1 calls 0.025 user 0.000 sys 0.026 real bam_write: 1 calls 0.082 user 0.000 sys 0.082 real execute: 1 calls 0.057 user 0.128 sys 85.595 real merge_files: 1 calls 0.038 user 0.100 sys 0.259 real parse_chunk: 2 calls 0.049 user 0.019 sys 0.082 real read_chunk: 7 calls 0.032 user 0.045 sys 6.210 real sort_block: 1 calls 0.154 user 0.045 sys 6.232 real write_chunk: 3 calls 0.009 user 0.016 sys 0.029 real overall: 463278080 mem 1.242 user 0.297 sys 85.642 real ls: cannot access '*.cram': No such file or directory