This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_tumor.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1530.363 CPU sec, 59.193 real sec [M::process] read 125052 sequences (17995734 bp) in 0.204 CPU sec, 0.214 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 43482, 0, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (180, 272, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (297.47, 149.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1032) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125052 reads in 20.876 CPU sec, 0.689 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125052 overall: 68477882368 mem 1382.922 user 174.085 sys 65.678 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_tumor.L006\tPU:L006\tSM:HCC1395_tumor\tLB:HCC1395_tumor\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_tumor_R1.fastq.gz HCC1395_tumor_R2.fastq.gz [main] Real time: 65.706 sec; CPU: 1557.010 sec sorting block 0, #reads = 125216 mem = 59825649 peak = 168398848 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 7277935 output reads: 125216 bam_mem_sort: 1 calls 0.028 user 0.000 sys 0.028 real bam_write: 1 calls 0.020 user 0.000 sys 0.021 real execute: 1 calls 0.090 user 0.121 sys 66.062 real merge_files: 1 calls 0.066 user 0.098 sys 0.295 real parse_chunk: 2 calls 0.049 user 0.037 sys 0.089 real read_chunk: 8 calls 0.015 user 0.051 sys 5.716 real sort_block: 1 calls 0.089 user 0.051 sys 5.662 real write_chunk: 4 calls 0.017 user 0.015 sys 0.037 real overall: 481910784 mem 1.429 user 0.354 sys 66.086 real ls: cannot access '*.cram': No such file or directory