cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_BL.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2485.804 CPU sec, 96.460 real sec [M::process] read 124700 sequences (17992902 bp) in 0.172 CPU sec, 0.175 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 44507, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 282, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (307.31, 153.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1070) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124700 reads in 40.678 CPU sec, 1.337 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124700 overall: 68353540096 mem 2031.273 user 504.781 sys 107.362 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_BL.L002\tPU:L002\tSM:HCC1395_BL\tLB:HCC1395_BL\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_BL_R1.fastq.gz HCC1395_BL_R2.fastq.gz [main] Real time: 107.418 sec; CPU: 2536.057 sec sorting block 0, #reads = 124816 mem = 59282090 peak = 163676160 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7313733 output reads: 124816 bam_mem_sort: 1 calls 0.027 user 0.000 sys 0.032 real bam_write: 1 calls 0.090 user 0.000 sys 0.089 real execute: 1 calls 0.049 user 0.138 sys 107.781 real merge_files: 1 calls 0.038 user 0.107 sys 0.261 real parse_chunk: 2 calls 0.038 user 0.035 sys 0.077 real read_chunk: 7 calls 0.040 user 0.044 sys 9.503 real sort_block: 1 calls 0.153 user 0.061 sys 9.542 real write_chunk: 3 calls 0.000 user 0.024 sys 0.035 real overall: 457719808 mem 1.259 user 0.347 sys 107.839 real ls: cannot access '*.cram': No such file or directory