This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_Blood.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1589.616 CPU sec, 65.650 real sec [M::process] read 125346 sequences (18175258 bp) in 0.209 CPU sec, 0.217 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 45082, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (192, 292, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (316.67, 156.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1100) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125346 reads in 20.928 CPU sec, 0.691 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125346 overall: 68484149248 mem 1411.677 user 206.161 sys 73.643 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_Blood.L004\tPU:L004\tSM:Sig_18_Blood\tLB:Sig_18_Blood\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_Blood_R1.fastq.gz Sig_18_Blood_R2.fastq.gz [main] Real time: 73.676 sec; CPU: 1617.840 sec sorting block 0, #reads = 125458 mem = 60226619 peak = 165277696 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 7445623 output reads: 125458 bam_mem_sort: 1 calls 0.029 user 0.000 sys 0.031 real bam_write: 1 calls 0.026 user 0.000 sys 0.024 real execute: 1 calls 0.050 user 0.160 sys 74.032 real merge_files: 1 calls 0.017 user 0.145 sys 0.301 real parse_chunk: 2 calls 0.067 user 0.019 sys 0.091 real read_chunk: 8 calls 0.014 user 0.056 sys 7.221 real sort_block: 1 calls 0.110 user 0.048 sys 7.181 real write_chunk: 4 calls 0.000 user 0.026 sys 0.032 real overall: 478883840 mem 1.496 user 0.362 sys 74.078 real ls: cannot access '*.cram': No such file or directory