Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/75/87c3fcf27ba11c4d2eda1391b071eb/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/af/58dfd778e724eef805a7f0a8ff9f5f/positive_somatic_control_2.snv_indel.merged.vcf.gz Downloading: s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta.fai Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/34/981bbb6fecd40b817ddc7930c036d0/Sig_18_tissue.recalibrated.bam Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/af/58dfd778e724eef805a7f0a8ff9f5f/positive_somatic_control_2.snv_indel.merged.vcf.gz.tbi Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/34/981bbb6fecd40b817ddc7930c036d0/Sig_18_tissue.recalibrated.bam.bai Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/75/87c3fcf27ba11c4d2eda1391b071eb/.command.run ==> STAGING COMPLETE (8 inputs) This is WhatsHap 2.8 running under Python 3.9.23 [W::hts_idx_load3] The index file is older than the data file: Sig_18_tissue.recalibrated.bam.bai [W::hts_idx_load3] The index file is older than the data file: Sig_18_tissue.recalibrated.bam.bai [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' [E::idx_find_and_load] Could not retrieve index file for 'input_filtered.vcf.gz' Working on 2 samples from 2 families # Working on contig chr5 in individual Sig_18_tissue Found 3 usable heterozygous variants (0 skipped due to missing genotypes) [W::hts_idx_load3] The index file is older than the data file: Sig_18_tissue.recalibrated.bam.bai Number of supplementary alignments: 0 Number of non-singleton groups: 0 Skipped 0 groups Found 0 reads covering 0 variants Kept 0 reads that cover at least two variants each Selected 0 most phase-informative reads covering 0 variants Best-case phasing would result in 0 non-singleton phased blocks (0 singletons). Phasing 1 sample by solving the MEC problem ... No. of phased blocks: 0 # Working on contig chr5 in individual reference-NA12878 Found 0 usable heterozygous variants (0 skipped due to missing genotypes) WARNING: Sample 'reference-NA12878' not found in any BAM/CRAM file. Found 0 reads covering 0 variants Kept 0 reads that cover at least two variants each Selected 0 most phase-informative reads covering 0 variants Best-case phasing would result in 0 non-singleton phased blocks (0 singletons). Phasing 1 sample by solving the MEC problem ... No. of phased blocks: 0 # Resource usage Maximum memory usage: 0.423 GB Time spent reading BAM/CRAM: 1.2 s Time spent parsing VCF: 0.0 s Time spent selecting reads: 0.0 s Time spent phasing: 0.0 s Time spent writing VCF: 0.0 s Time spent finding components: 0.0 s Time spent on rest: 0.0 s Total elapsed time: 1.2 s