This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_Blood.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1884.133 CPU sec, 69.702 real sec [M::process] read 125394 sequences (18178356 bp) in 0.179 CPU sec, 0.182 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 45026, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 291, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (315.20, 156.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125394 reads in 27.791 CPU sec, 0.914 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125394 overall: 68347289600 mem 1648.308 user 270.441 sys 77.442 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_Blood.L001\tPU:L001\tSM:Sig_18_Blood\tLB:Sig_18_Blood\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_Blood_R1.fastq.gz Sig_18_Blood_R2.fastq.gz [main] Real time: 77.471 sec; CPU: 1918.752 sec sorting block 0, #reads = 125537 mem = 60403849 peak = 160505856 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7527494 output reads: 125537 bam_mem_sort: 1 calls 0.024 user 0.000 sys 0.029 real bam_write: 1 calls 0.065 user 0.000 sys 0.061 real execute: 1 calls 0.062 user 0.132 sys 77.851 real merge_files: 1 calls 0.040 user 0.112 sys 0.275 real parse_chunk: 2 calls 0.070 user 0.017 sys 0.094 real read_chunk: 8 calls 0.052 user 0.047 sys 6.763 real sort_block: 1 calls 0.128 user 0.043 sys 6.764 real write_chunk: 4 calls 0.011 user 0.020 sys 0.036 real overall: 466677760 mem 1.375 user 0.290 sys 77.870 real ls: cannot access '*.cram': No such file or directory