This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_Blood.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2702.461 CPU sec, 107.669 real sec [M::process] read 124874 sequences (18107705 bp) in 0.173 CPU sec, 0.181 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44963, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (191, 290, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (315.00, 156.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1083) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124874 reads in 14.265 CPU sec, 0.473 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124874 overall: 67932012544 mem 2357.952 user 368.716 sys 118.103 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_Blood.L005\tPU:L005\tSM:Sig_18_Blood\tLB:Sig_18_Blood\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_Blood_R1.fastq.gz Sig_18_Blood_R2.fastq.gz [main] Real time: 118.133 sec; CPU: 2726.671 sec sorting block 0, #reads = 125029 mem = 60136694 peak = 163700736 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: 1f8bfbff fffab20b extended: f1bf27eb 1b407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7482925 output reads: 125029 bam_mem_sort: 1 calls 0.024 user 0.000 sys 0.025 real bam_write: 1 calls 0.081 user 0.000 sys 0.083 real execute: 1 calls 0.042 user 0.156 sys 118.527 real merge_files: 1 calls 0.030 user 0.122 sys 0.269 real parse_chunk: 2 calls 0.063 user 0.000 sys 0.073 real read_chunk: 8 calls 0.057 user 0.052 sys 9.898 real sort_block: 1 calls 0.152 user 0.043 sys 9.916 real write_chunk: 4 calls 0.000 user 0.026 sys 0.037 real overall: 468119552 mem 1.252 user 0.359 sys 118.546 real ls: cannot access '*.cram': No such file or directory