This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_BL.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2446.531 CPU sec, 92.163 real sec [M::process] read 124828 sequences (18022863 bp) in 0.173 CPU sec, 0.175 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44466, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 282, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (306.65, 153.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124828 reads in 48.758 CPU sec, 1.628 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124828 overall: 68186861568 mem 1939.694 user 566.439 sys 104.646 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_BL.L006\tPU:L006\tSM:HCC1395_BL\tLB:HCC1395_BL\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_BL_R1.fastq.gz HCC1395_BL_R2.fastq.gz [main] Real time: 104.676 sec; CPU: 2506.136 sec sorting block 0, #reads = 124937 mem = 59360983 peak = 163577856 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7327533 output reads: 124937 bam_mem_sort: 1 calls 0.022 user 0.000 sys 0.027 real bam_write: 1 calls 0.114 user 0.001 sys 0.113 real execute: 1 calls 0.047 user 0.152 sys 105.108 real merge_files: 1 calls 0.031 user 0.110 sys 0.258 real parse_chunk: 2 calls 0.055 user 0.013 sys 0.081 real read_chunk: 7 calls 0.029 user 0.040 sys 10.796 real sort_block: 1 calls 0.174 user 0.052 sys 10.856 real write_chunk: 3 calls 0.002 user 0.016 sys 0.039 real overall: 455352320 mem 1.158 user 0.421 sys 105.127 real ls: cannot access '*.cram': No such file or directory