cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o HCC1395_BL.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 1715.924 CPU sec, 59.910 real sec [M::process] read 125182 sequences (18067457 bp) in 0.211 CPU sec, 0.213 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 44339, 0, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (186, 284, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (307.42, 153.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 125182 reads in 17.555 CPU sec, 0.582 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 125182 overall: 68518690816 mem 1636.122 user 103.407 sys 66.547 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.HCC1395_BL.L004\tPU:L004\tSM:HCC1395_BL\tLB:HCC1395_BL\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 HCC1395_BL_R1.fastq.gz HCC1395_BL_R2.fastq.gz [main] Real time: 66.587 sec; CPU: 1739.533 sec sorting block 0, #reads = 125334 mem = 59594110 peak = 168452096 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000c06f2 features: 1f8bfbff fffab20b extended: f1bf07ab 1a407f7e amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) Platinum 8559C threads: 31 max 192 algo: util-sort license: sentieon:util=1 output file size: 7367948 output reads: 125334 bam_mem_sort: 1 calls 0.053 user 0.000 sys 0.053 real bam_write: 1 calls 0.050 user 0.000 sys 0.050 real execute: 1 calls 0.095 user 0.153 sys 67.093 real merge_files: 1 calls 0.069 user 0.127 sys 0.350 real parse_chunk: 2 calls 0.094 user 0.043 sys 0.153 real read_chunk: 7 calls 0.035 user 0.055 sys 6.014 real sort_block: 1 calls 0.156 user 0.074 sys 6.019 real write_chunk: 3 calls 0.017 user 0.025 sys 0.047 real overall: 465162240 mem 1.967 user 0.398 sys 67.115 real ls: cannot access '*.cram': No such file or directory