This software is licensed to bgold@natera.com by Sentieon Inc. cmdline: /opt/conda/share/sentieon-202308.03-3/libexec/util sort -r Homo_sapiens_assembly38.fasta -t 31 -o Sig_18_tissue.bam --sam2bam - This software is licensed to bgold@natera.com by Sentieon Inc. version: sentieon-genomics-202308.03 [M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::main_mem] bwa index loaded in 2323.466 CPU sec, 87.346 real sec [M::process] read 124902 sequences (17673402 bp) in 0.169 CPU sec, 0.170 real sec... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 46778, 1, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (154, 224, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (245.77, 118.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 124902 reads in 25.270 CPU sec, 0.830 real sec os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 algo: bwa-mem license: sentieon:klib=31 reads: 124902 overall: 68139626496 mem 1969.408 user 386.724 sys 95.577 real [main] Version: 0.7.17-r1188 [main] CMD: /opt/conda/share/sentieon-202308.03-3/libexec/bwa mem -K 100000000 -Y -R @RG\tID:REGRESSION.Sig_18_tissue.L004\tPU:L004\tSM:Sig_18_tissue\tLB:Sig_18_tissue\tDS:s3://natera-platform-sandbox/pipeline-resources/ngi-igenomes/igenomes/Homo_sapiens/GATK/GRCh38/Sequence/WholeGenomeFasta/Homo_sapiens_assembly38.fasta\tPL:ILLUMINA -t 31 ./BWAIndex/Homo_sapiens_assembly38.fasta.64 Sig_18_tissue_R1.fastq.gz Sig_18_tissue_R2.fastq.gz [main] Real time: 95.607 sec; CPU: 2356.135 sec sorting block 0, #reads = 125142 mem = 59607517 peak = 160505856 uniq header count 1 merging 1 blocks sw version: sentieon-genomics-202308.03 os version: Linux 6.12.88 cpu vendor: GenuineIntel signature: 000a06d1 features: bfebfbff 7ffefbff extended: f3bfb7ef fb417ffe amd bits: 2c100800 00000121 brand: Intel(R) Xeon(R) 6975P-C threads: 31 max 384 algo: util-sort license: sentieon:util=1 output file size: 7084194 output reads: 125142 bam_mem_sort: 1 calls 0.027 user 0.000 sys 0.031 real bam_write: 1 calls 0.114 user 0.002 sys 0.113 real execute: 1 calls 0.047 user 0.157 sys 96.039 real merge_files: 1 calls 0.032 user 0.120 sys 0.273 real parse_chunk: 2 calls 0.062 user 0.031 sys 0.102 real read_chunk: 8 calls 0.036 user 0.037 sys 7.347 real sort_block: 1 calls 0.171 user 0.053 sys 7.402 real write_chunk: 4 calls 0.012 user 0.014 sys 0.045 real overall: 466104320 mem 1.301 user 0.313 sys 96.058 real ls: cannot access '*.cram': No such file or directory