Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/62/4d6a7d5d1df89ad7c4a653cda87a2e/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/3a/d80c8d42fa53dbaf2fc88c128fb166/output/Sig_18_tissue_S3_L002_R1_001.fastq.gz
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/62/4d6a7d5d1df89ad7c4a653cda87a2e/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/3a/d80c8d42fa53dbaf2fc88c128fb166/output/Sig_18_tissue_S3_L002_R2_001.fastq.gz
==> STAGING COMPLETE (4 inputs)

Detecting adapter sequence for read1...
>Illumina TruSeq Adapter Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA

Detecting adapter sequence for read2...
>Illumina TruSeq Adapter Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT

Read1 before filtering:
total reads: 62626
total bases: 9393900
Q20 bases: 9233414(98.2916%)
Q30 bases: 8958586(95.366%)
Q40 bases: 8958586(95.366%)

Read2 before filtering:
total reads: 62626
total bases: 9393900
Q20 bases: 9176455(97.6853%)
Q30 bases: 8890809(94.6445%)
Q40 bases: 8890809(94.6445%)

Read1 after filtering:
total reads: 62622
total bases: 8871022
Q20 bases: 8768191(98.8408%)
Q30 bases: 8532404(96.1829%)
Q40 bases: 8532404(96.1829%)

Read2 after filtering:
total reads: 62622
total bases: 8871329
Q20 bases: 8710536(98.1875%)
Q30 bases: 8477302(95.5584%)
Q40 bases: 8477302(95.5584%)

Filtering result:
reads passed filter: 125244
reads failed due to low quality: 8
reads failed due to too many N: 0
reads failed due to too short: 0
reads with adapter trimmed: 28826
bases trimmed due to adapters: 1044546

Duplication rate: 0.718551%

Insert size peak (evaluated by paired-end reads): 171

JSON report: Sig_18_tissue.fastp.json
HTML report: Sig_18_tissue.fastp.html

fastp --in1 Sig_18_tissue_S3_L002_R1_001.fastq.gz --in2 Sig_18_tissue_S3_L002_R2_001.fastq.gz --out1 Sig_18_tissue/Sig_18_tissue_R1.fastq.gz --out2 Sig_18_tissue/Sig_18_tissue_R2.fastq.gz --detect_adapter_for_pe --length_required 15 --json Sig_18_tissue.fastp.json --html Sig_18_tissue.fastp.html --thread 4 
fastp v1.0.1, time used: 2 seconds