Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/0c/6b226d3a0c42ac8db27ee127eeef0c/rna_s1221_S42.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/09/b12062e5546550aded03288703fff3/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a4/929b22a9678e3de1d10e6342dd8d6e/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a4/929b22a9678e3de1d10e6342dd8d6e/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/bb/8c51e677048cb72c10a987d74941dc/rna_s1221_S42.Aligned.sortedByCoord.out.bam
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: rna_s1221_S42.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (560870) splicing reads. 94095 splicing junctions. 89669 known splicing junctions. 4426 novel splicing junctions.
sampling 10% (1121740) splicing reads. 113137 splicing junctions. 105076 known splicing junctions. 8061 novel splicing junctions.
sampling 15% (1682611) splicing reads. 124032 splicing junctions. 112857 known splicing junctions. 11175 novel splicing junctions.
sampling 20% (2243481) splicing reads. 131638 splicing junctions. 117735 known splicing junctions. 13903 novel splicing junctions.
sampling 25% (2804352) splicing reads. 137688 splicing junctions. 121318 known splicing junctions. 16370 novel splicing junctions.
sampling 30% (3365222) splicing reads. 142727 splicing junctions. 124077 known splicing junctions. 18650 novel splicing junctions.
sampling 35% (3926092) splicing reads. 147042 splicing junctions. 126276 known splicing junctions. 20766 novel splicing junctions.
sampling 40% (4486963) splicing reads. 150867 splicing junctions. 128189 known splicing junctions. 22678 novel splicing junctions.
sampling 45% (5047833) splicing reads. 154295 splicing junctions. 129732 known splicing junctions. 24563 novel splicing junctions.
sampling 50% (5608704) splicing reads. 157386 splicing junctions. 131177 known splicing junctions. 26209 novel splicing junctions.
sampling 55% (6169574) splicing reads. 160186 splicing junctions. 132448 known splicing junctions. 27738 novel splicing junctions.
sampling 60% (6730444) splicing reads. 162758 splicing junctions. 133584 known splicing junctions. 29174 novel splicing junctions.
sampling 65% (7291315) splicing reads. 165032 splicing junctions. 134575 known splicing junctions. 30457 novel splicing junctions.
sampling 70% (7852185) splicing reads. 167060 splicing junctions. 135413 known splicing junctions. 31647 novel splicing junctions.
sampling 75% (8413056) splicing reads. 168868 splicing junctions. 136210 known splicing junctions. 32658 novel splicing junctions.
sampling 80% (8973926) splicing reads. 170518 splicing junctions. 136888 known splicing junctions. 33630 novel splicing junctions.
sampling 85% (9534796) splicing reads. 172067 splicing junctions. 137535 known splicing junctions. 34532 novel splicing junctions.
sampling 90% (10095667) splicing reads. 173494 splicing junctions. 138067 known splicing junctions. 35427 novel splicing junctions.
sampling 95% (10656537) splicing reads. 174681 splicing junctions. 138562 known splicing junctions. 36119 novel splicing junctions.
sampling 100% (11217408) splicing reads. 175815 splicing junctions. 139001 known splicing junctions. 36814 novel splicing junctions.
null device 
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