Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/6f/56d11a69081e3d9778a45a2a8a3aa2/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ed/d7528d85624af8ded4c5b71932a777/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/ed/d7528d85624af8ded4c5b71932a777/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/c1/4c2e7e617359a2c6f2b5010c9c6a5c/rna_s1200_S21.Aligned.sortedByCoord.out.bam
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/47/3533ec2869c235ff8bfe2f26d1c54e/rna_s1200_S21.Aligned.sortedByCoord.out.bam.bai
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: rna_s1200_S21.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (586985) splicing reads. 95578 splicing junctions. 90874 known splicing junctions. 4704 novel splicing junctions.
sampling 10% (1173971) splicing reads. 114366 splicing junctions. 105982 known splicing junctions. 8384 novel splicing junctions.
sampling 15% (1760957) splicing reads. 125215 splicing junctions. 113561 known splicing junctions. 11654 novel splicing junctions.
sampling 20% (2347942) splicing reads. 133206 splicing junctions. 118626 known splicing junctions. 14580 novel splicing junctions.
sampling 25% (2934928) splicing reads. 139530 splicing junctions. 122316 known splicing junctions. 17214 novel splicing junctions.
sampling 30% (3521914) splicing reads. 144813 splicing junctions. 125151 known splicing junctions. 19662 novel splicing junctions.
sampling 35% (4108899) splicing reads. 149316 splicing junctions. 127485 known splicing junctions. 21831 novel splicing junctions.
sampling 40% (4695885) splicing reads. 153361 splicing junctions. 129405 known splicing junctions. 23956 novel splicing junctions.
sampling 45% (5282871) splicing reads. 156966 splicing junctions. 131086 known splicing junctions. 25880 novel splicing junctions.
sampling 50% (5869857) splicing reads. 160116 splicing junctions. 132503 known splicing junctions. 27613 novel splicing junctions.
sampling 55% (6456842) splicing reads. 163032 splicing junctions. 133755 known splicing junctions. 29277 novel splicing junctions.
sampling 60% (7043828) splicing reads. 165612 splicing junctions. 134871 known splicing junctions. 30741 novel splicing junctions.
sampling 65% (7630814) splicing reads. 167922 splicing junctions. 135836 known splicing junctions. 32086 novel splicing junctions.
sampling 70% (8217799) splicing reads. 170140 splicing junctions. 136750 known splicing junctions. 33390 novel splicing junctions.
sampling 75% (8804785) splicing reads. 172160 splicing junctions. 137575 known splicing junctions. 34585 novel splicing junctions.
sampling 80% (9391771) splicing reads. 173924 splicing junctions. 138297 known splicing junctions. 35627 novel splicing junctions.
sampling 85% (9978756) splicing reads. 175556 splicing junctions. 138966 known splicing junctions. 36590 novel splicing junctions.
sampling 90% (10565742) splicing reads. 177034 splicing junctions. 139542 known splicing junctions. 37492 novel splicing junctions.
sampling 95% (11152728) splicing reads. 178450 splicing junctions. 140105 known splicing junctions. 38345 novel splicing junctions.
sampling 100% (11739714) splicing reads. 179686 splicing junctions. 140590 known splicing junctions. 39096 novel splicing junctions.
null device 
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