Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/99/3a770d629a01ae2859a33b55ee2b45/rna_s1221_S42.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/86/299f4c23a9be8234a4ad64f1fff0e1/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2c/44fa176c41b6a521dce62c76986c44/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/2c/44fa176c41b6a521dce62c76986c44/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/76/2bd2c793c5591a9c9f87bd4f0439c4/rna_s1221_S42.Aligned.sortedByCoord.out.bam
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: rna_s1221_S42.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (560870) splicing reads. 93984 splicing junctions. 89604 known splicing junctions. 4380 novel splicing junctions.
sampling 10% (1121740) splicing reads. 112701 splicing junctions. 104873 known splicing junctions. 7828 novel splicing junctions.
sampling 15% (1682611) splicing reads. 123418 splicing junctions. 112531 known splicing junctions. 10887 novel splicing junctions.
sampling 20% (2243481) splicing reads. 131132 splicing junctions. 117404 known splicing junctions. 13728 novel splicing junctions.
sampling 25% (2804352) splicing reads. 137291 splicing junctions. 120976 known splicing junctions. 16315 novel splicing junctions.
sampling 30% (3365222) splicing reads. 142415 splicing junctions. 123764 known splicing junctions. 18651 novel splicing junctions.
sampling 35% (3926092) splicing reads. 146956 splicing junctions. 126154 known splicing junctions. 20802 novel splicing junctions.
sampling 40% (4486963) splicing reads. 150760 splicing junctions. 128066 known splicing junctions. 22694 novel splicing junctions.
sampling 45% (5047833) splicing reads. 154190 splicing junctions. 129781 known splicing junctions. 24409 novel splicing junctions.
sampling 50% (5608704) splicing reads. 157316 splicing junctions. 131292 known splicing junctions. 26024 novel splicing junctions.
sampling 55% (6169574) splicing reads. 160035 splicing junctions. 132513 known splicing junctions. 27522 novel splicing junctions.
sampling 60% (6730444) splicing reads. 162638 splicing junctions. 133676 known splicing junctions. 28962 novel splicing junctions.
sampling 65% (7291315) splicing reads. 164781 splicing junctions. 134568 known splicing junctions. 30213 novel splicing junctions.
sampling 70% (7852185) splicing reads. 166891 splicing junctions. 135465 known splicing junctions. 31426 novel splicing junctions.
sampling 75% (8413056) splicing reads. 168770 splicing junctions. 136193 known splicing junctions. 32577 novel splicing junctions.
sampling 80% (8973926) splicing reads. 170451 splicing junctions. 136878 known splicing junctions. 33573 novel splicing junctions.
sampling 85% (9534796) splicing reads. 171949 splicing junctions. 137466 known splicing junctions. 34483 novel splicing junctions.
sampling 90% (10095667) splicing reads. 173395 splicing junctions. 138049 known splicing junctions. 35346 novel splicing junctions.
sampling 95% (10656537) splicing reads. 174655 splicing junctions. 138553 known splicing junctions. 36102 novel splicing junctions.
sampling 100% (11217408) splicing reads. 175815 splicing junctions. 139001 known splicing junctions. 36814 novel splicing junctions.
null device 
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