FusionInspector.log - Workdir - NFCORE_RNAFUSION:RNAFUSION:FUSIONINSPECTOR_WORKFLOW:FUSIONINSPECTOR (rna_s1200_S21)

File Info

Filename: FusionInspector.log
Full Path: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/e1/8e6409e6192c8d22899f081f5d8c0a/FusionInspector.log
Size: 39.4 KB
Task: NFCORE_RNAFUSION:RNAFUSION:FUSIONINSPECTOR_WORKFLOW:FUSIONINSPECTOR (rna_s1200_S21)
Attempt
Content

2026-05-28 17:34:00,673 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_pair_to_mini_genome_join.pl --fusions rna_s1200_S21.fusionreport.tsv --gtf /tmp/nxf.I5kILhyNmf/ctat_genome_lib_build_dir/ref_annot.gtf --genome_fa /tmp/nxf.I5kILhyNmf/ctat_genome_lib_build_dir/ref_genome.fa --out_prefix /tmp/nxf.I5kILhyNmf/rna_s1200_S21 --shrink_introns --max_intron_length 1000 

2026-05-28 17:34:07,448 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.11 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_pair_to_mini_genome_join.pl --fusions rna_s1200_S21.fusionreport.tsv --gtf /tmp/nxf.I5kILhyNmf/ctat_genome_lib_build_dir/ref_annot.gtf --genome_fa /tmp/nxf.I5kILhyNmf/ctat_genome_lib_build_dir/ref_genome.fa --out_prefix /tmp/nxf.I5kILhyNmf/rna_s1200_S21 --shrink_introns --max_intron_length 1000 

2026-05-28 17:34:07,448 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/fusion_contigs.ok

2026-05-28 17:34:07,450 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: cp /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fa /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fa

2026-05-28 17:34:07,452 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fa /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fa

2026-05-28 17:34:07,452 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/cp_contigs_file_workdir

2026-05-28 17:34:07,453 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: cp /tmp/nxf.I5kILhyNmf/rna_s1200_S21.gtf /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.gtf

2026-05-28 17:34:07,455 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.I5kILhyNmf/rna_s1200_S21.gtf /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.gtf

2026-05-28 17:34:07,455 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/cp_gtf_file_workdir.ok

2026-05-28 17:34:07,457 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fasta_and_gtf_to_cytoband.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fa /tmp/nxf.I5kILhyNmf/rna_s1200_S21.gtf > /tmp/nxf.I5kILhyNmf/cytoBand.txt

2026-05-28 17:34:07,485 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fasta_and_gtf_to_cytoband.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fa /tmp/nxf.I5kILhyNmf/rna_s1200_S21.gtf > /tmp/nxf.I5kILhyNmf/cytoBand.txt

2026-05-28 17:34:07,485 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/cytoband.ok

2026-05-28 17:34:07,487 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/gtf_gene_to_bed.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21.gtf > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.bed

2026-05-28 17:34:07,569 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/gtf_gene_to_bed.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21.gtf > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.bed

2026-05-28 17:34:07,569 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/merged_contig_gtf_to_bed.ok

2026-05-28 17:34:07,571 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: sort -k1,1 -k2,2n /tmp/nxf.I5kILhyNmf/rna_s1200_S21.bed > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.bed.sorted.bed

2026-05-28 17:34:07,573 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: sort -k1,1 -k2,2n /tmp/nxf.I5kILhyNmf/rna_s1200_S21.bed > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.bed.sorted.bed

2026-05-28 17:34:07,573 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/rna_s1200_S21.bed.bedsort.ok

2026-05-28 17:34:07,574 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: bgzip -f /tmp/nxf.I5kILhyNmf/rna_s1200_S21.bed.sorted.bed

2026-05-28 17:34:07,581 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: bgzip -f /tmp/nxf.I5kILhyNmf/rna_s1200_S21.bed.sorted.bed

2026-05-28 17:34:07,581 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/rna_s1200_S21.bed.bgzip.ok

2026-05-28 17:34:07,583 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: tabix -p bed /tmp/nxf.I5kILhyNmf/rna_s1200_S21.bed.sorted.bed.gz

2026-05-28 17:34:07,588 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: tabix -p bed /tmp/nxf.I5kILhyNmf/rna_s1200_S21.bed.sorted.bed.gz

2026-05-28 17:34:07,589 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/rna_s1200_S21.bed.tabix.ok

2026-05-28 17:34:07,591 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: samtools faidx /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fa

2026-05-28 17:34:07,597 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: samtools faidx /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fa

2026-05-28 17:34:07,597 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/merged_contig_fai.ok

2026-05-28 17:34:07,598 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/run_FI_STAR.pl --genome /tmp/nxf.I5kILhyNmf/ctat_genome_lib_build_dir/ref_genome.fa --patch /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fa --max_mate_dist 100000 -G /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.gtf --CPU 8 --out_prefix rna_s1200_S21.star --out_dir /tmp/nxf.I5kILhyNmf/fi_workdir --reads "/tmp/nxf.I5kILhyNmf/rna_s1200_S21_1.fastp.fastq.gz /tmp/nxf.I5kILhyNmf/rna_s1200_S21_2.fastp.fastq.gz"

2026-05-28 17:42:26,113 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 8.31 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/run_FI_STAR.pl --genome /tmp/nxf.I5kILhyNmf/ctat_genome_lib_build_dir/ref_genome.fa --patch /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fa --max_mate_dist 100000 -G /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.gtf --CPU 8 --out_prefix rna_s1200_S21.star --out_dir /tmp/nxf.I5kILhyNmf/fi_workdir --reads "/tmp/nxf.I5kILhyNmf/rna_s1200_S21_1.fastp.fastq.gz /tmp/nxf.I5kILhyNmf/rna_s1200_S21_2.fastp.fastq.gz"

2026-05-28 17:42:26,113 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/run_STAR.ok

2026-05-28 17:42:26,115 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/bam_mark_duplicates.py  -i /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.sortedByCoord.out.bam   -o /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam   --remove_dups 

2026-05-28 17:42:26,483 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.01 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/bam_mark_duplicates.py  -i /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.sortedByCoord.out.bam   -o /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam   --remove_dups 

2026-05-28 17:42:26,483 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/mark_dup_reads.ok

2026-05-28 17:42:26,485 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: samtools index /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam

2026-05-28 17:42:26,525 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: samtools index /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam

2026-05-28 17:42:26,526 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/mark_dups_reads.index.ok

2026-05-28 17:42:26,527 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_JUNCTION_reads_from_fusion_contig_bam.pl --gtf_file /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam

2026-05-28 17:42:27,982 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.02 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_JUNCTION_reads_from_fusion_contig_bam.pl --gtf_file /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam

2026-05-28 17:42:27,982 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok

2026-05-28 17:42:27,984 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_SPANNING_reads_from_bam.from_chim_summary.pl --gtf_file /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam --junction_info /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam.fusion_junction_info --genome_lib_dir ctat_genome_lib_build_dir   > /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam.fusion_span_reads.sam

2026-05-28 17:42:32,901 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.08 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_SPANNING_reads_from_bam.from_chim_summary.pl --gtf_file /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam --junction_info /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam.fusion_junction_info --genome_lib_dir ctat_genome_lib_build_dir   > /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam.fusion_span_reads.sam

2026-05-28 17:42:32,902 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok

2026-05-28 17:42:32,904 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/coalesce_junction_and_spanning_info.pl /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam.fusion_junction_info /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam.fusion_spanning_info 1  > /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary

2026-05-28 17:42:32,919 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/coalesce_junction_and_spanning_info.pl /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam.fusion_junction_info /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam.fusion_spanning_info 1  > /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary

2026-05-28 17:42:32,920 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/coalesce_junc_n_span.ok

2026-05-28 17:42:32,921 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary > /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.EMadj

2026-05-28 17:42:32,935 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary > /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.EMadj

2026-05-28 17:42:32,936 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/init_EM_adj_counts.ok

2026-05-28 17:42:32,937 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/filter_fusions_by_frag_thresholds.pl --min_junction_reads 0 --min_sum_frags 1 --min_novel_junction_support 3 --min_spanning_frags_only 5 --fusion_preds /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.EMadj --require_LDAS 1 > /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.EMadj.min_frag_thresh

2026-05-28 17:42:32,955 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/filter_fusions_by_frag_thresholds.pl --min_junction_reads 0 --min_sum_frags 1 --min_novel_junction_support 3 --min_spanning_frags_only 5 --fusion_preds /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.EMadj --require_LDAS 1 > /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.EMadj.min_frag_thresh

2026-05-28 17:42:32,955 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/filter_by_frag_threshs.ok

2026-05-28 17:42:32,957 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary LeftGene,RightGene,JunctionReads  > /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.fusion_junction_read_accs

2026-05-28 17:42:32,970 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary LeftGene,RightGene,JunctionReads  > /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.fusion_junction_read_accs

2026-05-28 17:42:32,970 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/prep_igv_extract_junc_reads.ok

2026-05-28 17:42:32,972 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: bash -c "set -eof pipefail; /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_junction_reads_by_accession.pl /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.fusion_junction_read_accs /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam | samtools view -@ 8 -bT /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fa -  | samtools sort -@ 8 - -o /tmp/nxf.I5kILhyNmf/rna_s1200_S21.junction_reads.bam"

2026-05-28 17:42:33,012 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: bash -c "set -eof pipefail; /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_junction_reads_by_accession.pl /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.fusion_junction_read_accs /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam | samtools view -@ 8 -bT /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fa -  | samtools sort -@ 8 - -o /tmp/nxf.I5kILhyNmf/rna_s1200_S21.junction_reads.bam"

2026-05-28 17:42:33,013 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/prep_igv_junc_reads_bam.ok

2026-05-28 17:42:33,014 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: samtools index /tmp/nxf.I5kILhyNmf/rna_s1200_S21.junction_reads.bam

2026-05-28 17:42:33,027 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: samtools index /tmp/nxf.I5kILhyNmf/rna_s1200_S21.junction_reads.bam

2026-05-28 17:42:33,027 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/samtools_idx_junc_reads_bam.ok

2026-05-28 17:42:33,029 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21.junction_reads.bam > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.junction_reads.bam.bed

2026-05-28 17:42:33,049 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21.junction_reads.bam > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.junction_reads.bam.bed

2026-05-28 17:42:33,049 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/rna_s1200_S21.junction_reads.bam.bam_to_bed.ok

2026-05-28 17:42:33,051 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: sort -k1,1 -k2,2n /tmp/nxf.I5kILhyNmf/rna_s1200_S21.junction_reads.bam.bed > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.junction_reads.bam.bed.sorted.bed

2026-05-28 17:42:33,053 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: sort -k1,1 -k2,2n /tmp/nxf.I5kILhyNmf/rna_s1200_S21.junction_reads.bam.bed > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.junction_reads.bam.bed.sorted.bed

2026-05-28 17:42:33,053 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/rna_s1200_S21.junction_reads.bam.bedsort.ok

2026-05-28 17:42:33,054 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: bgzip -f /tmp/nxf.I5kILhyNmf/rna_s1200_S21.junction_reads.bam.bed.sorted.bed

2026-05-28 17:42:33,060 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: bgzip -f /tmp/nxf.I5kILhyNmf/rna_s1200_S21.junction_reads.bam.bed.sorted.bed

2026-05-28 17:42:33,060 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/rna_s1200_S21.junction_reads.bam.bgzip.ok

2026-05-28 17:42:33,062 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: tabix -p bed /tmp/nxf.I5kILhyNmf/rna_s1200_S21.junction_reads.bam.bed.sorted.bed.gz

2026-05-28 17:42:33,075 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: tabix -p bed /tmp/nxf.I5kILhyNmf/rna_s1200_S21.junction_reads.bam.bed.sorted.bed.gz

2026-05-28 17:42:33,075 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/rna_s1200_S21.junction_reads.bam.tabix.ok

2026-05-28 17:42:33,077 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary LeftGene,RightGene,SpanningFrags  > /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.fusion_spanning_read_accs

2026-05-28 17:42:33,090 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary LeftGene,RightGene,SpanningFrags  > /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.fusion_spanning_read_accs

2026-05-28 17:42:33,090 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/span_reads_acc.ok

2026-05-28 17:42:33,092 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: samtools view -H /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam -b -o /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam

2026-05-28 17:42:33,097 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: samtools view -H /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam -b -o /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam

2026-05-28 17:42:33,097 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/init_spanning_reads_bam.ok

2026-05-28 17:42:33,099 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_spanning_reads_by_accession.pl /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.fusion_spanning_read_accs /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam.fusion_span_reads.sam | samtools view -@ 8 -bT /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fa -  | samtools sort -@ 8 - -o /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam || : 

2026-05-28 17:42:33,135 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_spanning_reads_by_accession.pl /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.fusion_spanning_read_accs /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam.fusion_span_reads.sam | samtools view -@ 8 -bT /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fa -  | samtools sort -@ 8 - -o /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam || : 

2026-05-28 17:42:33,135 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/prep_spanning_reads.ok

2026-05-28 17:42:33,137 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: samtools index /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam

2026-05-28 17:42:33,145 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: samtools index /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam

2026-05-28 17:42:33,145 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/samtools_index_span_reads_bam.ok

2026-05-28 17:42:33,147 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam.bed

2026-05-28 17:42:33,163 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam.bed

2026-05-28 17:42:33,163 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/rna_s1200_S21.spanning_reads.bam.bam_to_bed.ok

2026-05-28 17:42:33,165 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: sort -k1,1 -k2,2n /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam.bed > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam.bed.sorted.bed

2026-05-28 17:42:33,166 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: sort -k1,1 -k2,2n /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam.bed > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam.bed.sorted.bed

2026-05-28 17:42:33,166 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/rna_s1200_S21.spanning_reads.bam.bedsort.ok

2026-05-28 17:42:33,167 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: bgzip -f /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam.bed.sorted.bed

2026-05-28 17:42:33,176 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: bgzip -f /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam.bed.sorted.bed

2026-05-28 17:42:33,176 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/rna_s1200_S21.spanning_reads.bam.bgzip.ok

2026-05-28 17:42:33,178 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: tabix -p bed /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam.bed.sorted.bed.gz

2026-05-28 17:42:33,185 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: tabix -p bed /tmp/nxf.I5kILhyNmf/rna_s1200_S21.spanning_reads.bam.bed.sorted.bed.gz

2026-05-28 17:42:33,185 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/rna_s1200_S21.spanning_reads.bam.tabix.ok

2026-05-28 17:42:33,187 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: cp /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam /tmp/nxf.I5kILhyNmf/rna_s1200_S21.consolidated.bam

2026-05-28 17:42:33,190 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.star.cSorted.dupsMarked.bam /tmp/nxf.I5kILhyNmf/rna_s1200_S21.consolidated.bam

2026-05-28 17:42:33,190 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/cp_consol_bam.ok

2026-05-28 17:42:33,191 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: samtools index /tmp/nxf.I5kILhyNmf/rna_s1200_S21.consolidated.bam

2026-05-28 17:42:33,229 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: samtools index /tmp/nxf.I5kILhyNmf/rna_s1200_S21.consolidated.bam

2026-05-28 17:42:33,229 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/index_consol_bam.ok

2026-05-28 17:42:33,231 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_to_frag_coords.pl --sam /tmp/nxf.I5kILhyNmf/rna_s1200_S21.consolidated.bam --max_insert_size 10000000 

2026-05-28 17:42:33,959 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.01 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_to_frag_coords.pl --sam /tmp/nxf.I5kILhyNmf/rna_s1200_S21.consolidated.bam --max_insert_size 10000000 

2026-05-28 17:42:33,959 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/prep_igv_sam_frag_coordss.ok

2026-05-28 17:42:33,961 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_summary_to_igv_JuncSpan_fmt.pl /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary /tmp/nxf.I5kILhyNmf/rna_s1200_S21.consolidated.bam.frag_coords > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.igv.FusionJuncSpan

2026-05-28 17:42:33,985 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_summary_to_igv_JuncSpan_fmt.pl /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary /tmp/nxf.I5kILhyNmf/rna_s1200_S21.consolidated.bam.frag_coords > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.igv.FusionJuncSpan

2026-05-28 17:42:33,985 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/prep_igv_juncspan_fmt.ok

2026-05-28 17:42:33,987 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_pfam_domain_info.pl --finspector_gtf /tmp/nxf.I5kILhyNmf/rna_s1200_S21.gtf  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.igv.Pfam.gff3 

2026-05-28 17:42:34,050 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_pfam_domain_info.pl --finspector_gtf /tmp/nxf.I5kILhyNmf/rna_s1200_S21.gtf  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.igv.Pfam.gff3 

2026-05-28 17:42:34,050 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/prep_igv_pfam_gff3.ok

2026-05-28 17:42:34,052 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21.igv.Pfam.gff3 > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.igv.Pfam.bed

2026-05-28 17:42:34,087 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21.igv.Pfam.gff3 > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.igv.Pfam.bed

2026-05-28 17:42:34,088 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/prep_igv_pfam_bed.ok

2026-05-28 17:42:34,089 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_seq_similar_region_FI_coordinates.pl --finspector_gtf /tmp/nxf.I5kILhyNmf/rna_s1200_S21.gtf  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.igv.seqsimilar.gff3 

2026-05-28 17:42:34,115 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_seq_similar_region_FI_coordinates.pl --finspector_gtf /tmp/nxf.I5kILhyNmf/rna_s1200_S21.gtf  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.igv.seqsimilar.gff3 

2026-05-28 17:42:34,116 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/prep_igv_seqsim_gff3.ok

2026-05-28 17:42:34,117 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21.igv.seqsimilar.gff3 > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.igv.seqsimilar.bed

2026-05-28 17:42:34,138 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21.igv.seqsimilar.gff3 > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.igv.seqsimilar.bed

2026-05-28 17:42:34,138 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/prep_igv_seqsim_bed.ok

2026-05-28 17:42:34,140 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/append_breakpoint_junction_info_via_FI_contigs.pl /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.EMadj.min_frag_thresh /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fa > /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo

2026-05-28 17:42:34,164 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/append_breakpoint_junction_info_via_FI_contigs.pl /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.EMadj.min_frag_thresh /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fa > /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo

2026-05-28 17:42:34,164 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/add_splice_info.ok

2026-05-28 17:42:34,166 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/blast_and_promiscuity_filter.pl  --fusion_preds /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --out_prefix /tmp/nxf.I5kILhyNmf/rna_s1200_S21 --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50

2026-05-28 17:42:34,330 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/blast_and_promiscuity_filter.pl  --fusion_preds /tmp/nxf.I5kILhyNmf/fi_workdir/rna_s1200_S21.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --out_prefix /tmp/nxf.I5kILhyNmf/rna_s1200_S21 --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50

2026-05-28 17:42:34,331 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/blast_filter.ok

2026-05-28 17:42:34,332 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/util/filter_by_annotation_rules.pl  --fusions /tmp/nxf.I5kILhyNmf/rna_s1200_S21.post_blast_and_promiscuity_filter --genome_lib_dir /tmp/nxf.I5kILhyNmf/ctat_genome_lib_build_dir

2026-05-28 17:42:34,351 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/util/filter_by_annotation_rules.pl  --fusions /tmp/nxf.I5kILhyNmf/rna_s1200_S21.post_blast_and_promiscuity_filter --genome_lib_dir /tmp/nxf.I5kILhyNmf/ctat_genome_lib_build_dir

2026-05-28 17:42:34,351 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/annot_filter.ok

2026-05-28 17:42:34,353 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21.post_blast_and_promiscuity_filter.annot_filter.pass > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj

2026-05-28 17:42:34,367 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21.post_blast_and_promiscuity_filter.annot_filter.pass > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj

2026-05-28 17:42:34,367 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/EM_adj_counts.ok

2026-05-28 17:42:34,368 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/incorporate_FFPM_into_final_report.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21_1.fastp.fastq.gz /tmp/nxf.I5kILhyNmf/rna_s1200_S21.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM

2026-05-28 17:42:47,390 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.22 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/incorporate_FFPM_into_final_report.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21_1.fastp.fastq.gz /tmp/nxf.I5kILhyNmf/rna_s1200_S21.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM

2026-05-28 17:42:47,390 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/add_FFPM.ok

2026-05-28 17:42:47,392 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/find_microhomologies_by_kmer_matches.pl --fasta /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fa --gtf /tmp/nxf.I5kILhyNmf/rna_s1200_S21.gtf  > /tmp/nxf.I5kILhyNmf/fi_workdir/microH.dat

2026-05-28 17:42:47,453 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/find_microhomologies_by_kmer_matches.pl --fasta /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fa --gtf /tmp/nxf.I5kILhyNmf/rna_s1200_S21.gtf  > /tmp/nxf.I5kILhyNmf/fi_workdir/microH.dat

2026-05-28 17:42:47,453 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/microH.dat.ok

2026-05-28 17:42:47,455 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/append_microH_distance.py /tmp/nxf.I5kILhyNmf/fi_workdir/microH.dat /tmp/nxf.I5kILhyNmf/rna_s1200_S21.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM  >  /tmp/nxf.I5kILhyNmf/rna_s1200_S21.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH

2026-05-28 17:42:47,477 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/append_microH_distance.py /tmp/nxf.I5kILhyNmf/fi_workdir/microH.dat /tmp/nxf.I5kILhyNmf/rna_s1200_S21.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM  >  /tmp/nxf.I5kILhyNmf/rna_s1200_S21.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH

2026-05-28 17:42:47,477 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/append_microH_info.ok

2026-05-28 17:42:47,478 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: cp /tmp/nxf.I5kILhyNmf/rna_s1200_S21.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH /tmp/nxf.I5kILhyNmf/rna_s1200_S21.FusionInspector.fusions.tsv

2026-05-28 17:42:47,480 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.I5kILhyNmf/rna_s1200_S21.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH /tmp/nxf.I5kILhyNmf/rna_s1200_S21.FusionInspector.fusions.tsv

2026-05-28 17:42:47,480 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/cp_final.ok

2026-05-28 17:42:47,482 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/column_exclusions.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21.FusionInspector.fusions.tsv JunctionReads,SpanningFrags,CounterFusionLeftReads,CounterFusionRightReads  > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.FusionInspector.fusions.abridged.tsv

2026-05-28 17:42:47,495 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/column_exclusions.pl /tmp/nxf.I5kILhyNmf/rna_s1200_S21.FusionInspector.fusions.tsv JunctionReads,SpanningFrags,CounterFusionLeftReads,CounterFusionRightReads  > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.FusionInspector.fusions.abridged.tsv

2026-05-28 17:42:47,495 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/final.abridged.ok

2026-05-28 17:42:47,497 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/util/create_fusion_inspector_igvjs.py --fusion_inspector_directory /tmp/nxf.I5kILhyNmf --json_outfile /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fusion_inspector_web.json --file_prefix rna_s1200_S21

2026-05-28 17:42:47,517 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/create_fusion_inspector_igvjs.py --fusion_inspector_directory /tmp/nxf.I5kILhyNmf --json_outfile /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fusion_inspector_web.json --file_prefix rna_s1200_S21

2026-05-28 17:42:47,517 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/create_fi_igvjs.ok

2026-05-28 17:42:47,519 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/fusion-reports/create_fusion_report.py --html_template /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_report_html_template/igvjs_fusion.html --fusions_json /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fusion_inspector_web.json --input_file_prefix rna_s1200_S21 --html_output /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fusion_inspector_web.html

2026-05-28 17:42:47,823 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.01 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/fusion-reports/create_fusion_report.py --html_template /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_report_html_template/igvjs_fusion.html --fusions_json /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fusion_inspector_web.json --input_file_prefix rna_s1200_S21 --html_output /tmp/nxf.I5kILhyNmf/rna_s1200_S21.fusion_inspector_web.html

2026-05-28 17:42:47,824 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/fusion_reports_html.ok

2026-05-28 17:42:47,826 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/FusionAnnotator --annotate /tmp/nxf.I5kILhyNmf/rna_s1200_S21.FusionInspector.fusions.abridged.tsv  --genome_lib_dir /tmp/nxf.I5kILhyNmf/ctat_genome_lib_build_dir > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.FusionInspector.fusions.abridged.tsv.annotated 

2026-05-28 17:42:47,848 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/FusionAnnotator --annotate /tmp/nxf.I5kILhyNmf/rna_s1200_S21.FusionInspector.fusions.abridged.tsv  --genome_lib_dir /tmp/nxf.I5kILhyNmf/ctat_genome_lib_build_dir > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.FusionInspector.fusions.abridged.tsv.annotated 

2026-05-28 17:42:47,848 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/fusion_annotator.ok

2026-05-28 17:42:47,850 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/util/fusion_to_coding_region_effect.pl --fusions /tmp/nxf.I5kILhyNmf/rna_s1200_S21.FusionInspector.fusions.abridged.tsv.annotated  --genome_lib_dir /tmp/nxf.I5kILhyNmf/ctat_genome_lib_build_dir > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.FusionInspector.fusions.abridged.tsv.annotated.coding_effect 

2026-05-28 17:42:47,928 INFO Pipeliner.Pipeliner.run at 136 :
	Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/util/fusion_to_coding_region_effect.pl --fusions /tmp/nxf.I5kILhyNmf/rna_s1200_S21.FusionInspector.fusions.abridged.tsv.annotated  --genome_lib_dir /tmp/nxf.I5kILhyNmf/ctat_genome_lib_build_dir > /tmp/nxf.I5kILhyNmf/rna_s1200_S21.FusionInspector.fusions.abridged.tsv.annotated.coding_effect 

2026-05-28 17:42:47,928 INFO Pipeliner.Pipeliner.run_cmd at 21 :
	Running: touch /tmp/nxf.I5kILhyNmf/chckpts_dir/fusion_coding_region_effect.ok